Plant Biotechnology Journal最新文献_第9页

Cross-Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana-Derived WUS2 Developmental Regulator. 香蕉衍生的WUS2发育调控因子对发育可塑性和代谢重编程的影响。

IF 10.5 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-12 DOI: 10.1111/pbi.70625

Roni Chaudhary, Surender Singh, Usman Ali, Siddharth Tiwari

{"title":"Cross-Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana-Derived WUS2 Developmental Regulator.","authors":"Roni Chaudhary, Surender Singh, Usman Ali, Siddharth Tiwari","doi":"10.1111/pbi.70625","DOIUrl":"https://doi.org/10.1111/pbi.70625","url":null,"abstract":"Plant regeneration is governed by intrinsic gene regulation and phytohormonal cues. WUSCHEL (WUS) gene promotes regeneration, but its broader functional role remains unexplored. Here, we demonstrate that the constitutive and inducible expression of banana-derived WUS2 (GN-WUS2) enhances regeneration in Nicotiana tabacum (tobacco) and Musa acuminata (banana) cv. Grand Naine, even in hormone-free MS medium. Constitutive (CaMV35S::GN-WUS2) expression promoted shoot formation and modulated hormonal and morphogenic gene expression, as evidenced by molecular, biochemical and histological analyses. However, it caused some pleiotropic effects. To overcome this, glucocorticoid receptor-based inducible GN-WUS2 expression enabled healthy shoot development. The upregulated expression of HMGR1, IPPI2 and SMT1-2 in transgenic tobacco lines boosted isoprenoid and phytosterol biosynthesis and correlated with increased cell division, biomass, pod size and seed yield. Proteomic analysis of seeds from transgenic tobacco lines revealed an enrichment of lipid-associated proteins and the accumulation of the novel lipid adipic acid, supported by expression profiling of NtKA and NtSA genes. Collectively, these results establish GN-WUS2 as a master regulator that integrates developmental reprogramming with novel phytosterol biosynthesis and yield enhancement, presenting its versatile role in next-generation regeneration and crop improvement.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The BAHD Acyltransferase Gene Family: Evolutionary Dynamics, Biochemical Mechanisms, and Roles in Plant Stress Adaptation. BAHD酰基转移酶基因家族：进化动力学、生化机制及其在植物逆境适应中的作用

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-11 DOI: 10.1111/pbi.70597

Muhammad Mubashar Zafar,Qiao Fei,Abdul Razzaq,Ayesha Siddiqua,Ayesha Naveed,M Nasir Khan,Huma Saleem,Xuefei Jiang

{"title":"The BAHD Acyltransferase Gene Family: Evolutionary Dynamics, Biochemical Mechanisms, and Roles in Plant Stress Adaptation.","authors":"Muhammad Mubashar Zafar,Qiao Fei,Abdul Razzaq,Ayesha Siddiqua,Ayesha Naveed,M Nasir Khan,Huma Saleem,Xuefei Jiang","doi":"10.1111/pbi.70597","DOIUrl":"https://doi.org/10.1111/pbi.70597","url":null,"abstract":"BAHD acyltransferases constitute one of the most versatile enzyme superfamilies in plants, catalysing the acylation of alcohols, amines, polyamines, and phenolic compounds to generate an extraordinary diversity of specialised metabolites. Initially identified through a limited number of anthocyanin- and alkaloid-modifying enzymes, BAHDs are now recognised as key regulators of phenylpropanoid flux, cutin and suberin polymerisation, volatile ester biosynthesis, and the stabilisation of acylated flavonoids. Comparative genomic analyses classify BAHD proteins into eight clades that share conserved catalytic motifs yet display pronounced functional divergence, reflecting a balance between deep evolutionary conservation and lineage-specific innovation. Recent structural and biochemical studies demonstrate how subtle active-site modifications govern substrate promiscuity and specialisation, enabling rapid metabolic reprogramming during environmental stress. Omics-based investigations further reveal widespread induction of BAHD genes under drought, salinity, heat stress, pathogen attack, and herbivory, linking BAHD activity to cell wall reinforcement, phenolamide biosynthesis, anthocyanin acylation, and ecological signalling. Beyond their physiological roles, BAHD acyltransferases have emerged as attractive targets for metabolic engineering, synthetic biology, and crop improvement, where manipulation of specific family members enhances stress tolerance, biomass quality, and nutritional or industrial value. Here, we integrate evolutionary, structural, and regulatory insights into BAHD function, highlight emerging translational opportunities, and discuss challenges associated with functional redundancy, substrate promiscuity, and biosafety considerations. Collectively, this synthesis positions BAHD acyltransferases as central mediators of plant adaptation and as promising tools for sustainable agriculture and biotechnological innovation.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"54 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147383502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical Strength: An Unrecognised Target in the Genetic Improvement of Crops. 机械强度：作物遗传改良中一个未被认识的目标。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-11 DOI: 10.1111/pbi.70627

Qingbiao Shi,Qibin Wang,Guodong Wang,Yiduo An,Hengjia Yang,Qing Tao,Ying Xia,Zihao Jiao,Naiqian Li,Ran Gao,Junfen Li,Fanying Kong,Haisen Zhang,Pinghua Li,Mingyue Gou,Haiyang Wang,Bosheng Li,Gang Li

{"title":"Mechanical Strength: An Unrecognised Target in the Genetic Improvement of Crops.","authors":"Qingbiao Shi,Qibin Wang,Guodong Wang,Yiduo An,Hengjia Yang,Qing Tao,Ying Xia,Zihao Jiao,Naiqian Li,Ran Gao,Junfen Li,Fanying Kong,Haisen Zhang,Pinghua Li,Mingyue Gou,Haiyang Wang,Bosheng Li,Gang Li","doi":"10.1111/pbi.70627","DOIUrl":"https://doi.org/10.1111/pbi.70627","url":null,"abstract":"Leaf angle (LA) is a crucial agronomic trait influencing planting density and crop yield. Previous research highlighted the importance of cellular variations in the ligular region for determining LA, but the underlying regulatory mechanisms remain unclear. Here, we demonstrate LA is not a static trait, but rather represents a dynamic equilibrium between mechanical forces maintaining leaf erectness and those promoting blade drooping. To quantify the drooping tendency, we introduce gravitational moments, which show positive correlations with LA, blade length (BL) and blade weight (BWt). Notably, the mechanical forces are tightly regulated by the sheath layers surrounding the stalk and by the thickness and lignin deposition on the ligular region of sclerenchyma (SC) cells. Furthermore, we applied single-nucleus transcriptome analyses (snRNA-seq) to construct a comprehensive transcriptional atlas spanning the ligular regions of compact-type (Z58), intermediate-type (B73) and expanded-type (W22) inbred lines. Through the comparative analysis of snRNA-seq and RNA-seq of three inbred lines, we identified the adaxial hypodermis (HP) cells as pivotal sites where lignin biogenesis and metabolism genes were specifically expressed in compact-type Z58, consistent with the lignin deposition pattern. Notably, we discovered that the NAM, ATAF and CUC (NAC) transcription factor-encoding genes NAC secondary wall thickening promoting factor 2 (NST2) and NST3, which mediate lignin biogenesis in the ligular region, especially on the adaxial side, play key roles in reinforcing mechanical support and reducing LA. Collectively, this study advances our understanding of ligular development and LA regulatory mechanisms and provides strategic insights for breeding crops with improved agricultural productivity.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"54 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147393976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsRALF26 Serves as an Endogenous Signal Recognised by XA21 to Promote Robust and Distal Resistance in Rice. OsRALF26作为XA21识别的内源信号促进水稻的强抗性和远端抗性

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-11 DOI: 10.1111/pbi.70622

Oh-Kyu Kwon,A-Ram Jeong,Chang-Jin Park

{"title":"OsRALF26 Serves as an Endogenous Signal Recognised by XA21 to Promote Robust and Distal Resistance in Rice.","authors":"Oh-Kyu Kwon,A-Ram Jeong,Chang-Jin Park","doi":"10.1111/pbi.70622","DOIUrl":"https://doi.org/10.1111/pbi.70622","url":null,"abstract":"Plant immune receptors detect both microbe-derived and endogenous signals to activate defences. XA21, a rice immune receptor, confers strong race-specific resistance to a subset of Xanthomonas oryzae pv. oryzae (Xoo) strains by recognising the microbial sulphated peptide RaxX. However, the molecular basis for the notably robust XA21-mediated immune response has remained unclear. Here, we report that the small secreted peptide OsRALF26, previously identified as an Oryza-specific ligand for FERONIA-like receptor 1 (OsFLR1), is also directly perceived by XA21. Recognition of OsRALF26 by XA21 triggers a pronounced reactive oxygen species (ROS) burst, pathogenesis-related (PR) gene induction, and enhanced resistance to Xoo. Notably, silencing OsRALF26 leads to a spatially biased reduction in XA21-mediated resistance, particularly in distal tissues. These findings identify OsRALF26 as a host-derived ligand of XA21 that is required for full activation of XA21-mediated immunity in distal tissues, consistent with a role for OsRALF26 in spatial propagation of XA21-dependent defence. By integrating microbe-derived and endogenous signals, XA21 exemplifies a versatile immune strategy in rice. This dual recognition may have arisen through the introgression of XA21, which unintentionally conferred OsRALF26 responsiveness-thereby reinforcing immune robustness in rice varieties.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"45 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147383501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Enzyme MeHNL11 Regulates MeCAS1b Transcription for Cyanide Reutilization in Response to Nitrate Deficiency in Cassava. 代谢酶MeHNL11调控木薯氰化物再利用的MeCAS1b转录

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-10 DOI: 10.1111/pbi.70633

Weitao Mai,Ruxue Bao,Xiaocheng Liu,Mengtao Li,Jinling Zhao,Huaifang Zhang,Yuan Yao,Haiyan Wang,Wenquan Wang,Changying Zeng,Xin Chen

{"title":"Metabolic Enzyme MeHNL11 Regulates MeCAS1b Transcription for Cyanide Reutilization in Response to Nitrate Deficiency in Cassava.","authors":"Weitao Mai,Ruxue Bao,Xiaocheng Liu,Mengtao Li,Jinling Zhao,Huaifang Zhang,Yuan Yao,Haiyan Wang,Wenquan Wang,Changying Zeng,Xin Chen","doi":"10.1111/pbi.70633","DOIUrl":"https://doi.org/10.1111/pbi.70633","url":null,"abstract":"Cassava (Manihot esculenta Crantz) exhibits exceptional tolerance to infertile soils and contains abundant cyanogenic glucosides (CGs). Previous research has indicated that CGs can serve as a significant reservoir of organic nitrogen in plants. However, the extent to which its high-CG content contributes to efficient nitrogen utilisation and adaptation to low nitrogen (N) in cassava remains to be further elucidated. This study represents the first identification of MeHNL11 as a bifunctional protein. In response to N deficiency, the hydroxynitrile lyase activity of MeHNL11 promotes the generation and accumulation of cyanide and the Cys245 residue of MeHNL11 is critical for its nuclear oligomerization, in which the protein functions as a transcription factor. Following the cyanide transmission into the nucleus, the oligomeric form of MeHNL11 dissociates into monomers, leading to a dramatic upregulation of MeCAS1b transcription. This regulatory mechanism helps sustain intracellular cyanide homeostasis within cassava and facilitates the synthesis of primary N metabolites, thereby alleviating N deficiency. The exogenous application of the cyanide antidote hydroxocobalamin (COB) inhibited cyanide assimilation by MeCAS1b, leading to exacerbated N deficiency symptoms, such as leaf yellowing and a significant reduction in the contents of NH4 + and free amino acids (AA) in cassava seedlings under low-N conditions (LN). Our research demonstrates that the MeHNL11-MeCAS1b module plays a pivotal role in CG recycling, offering new insights into the underlying mechanisms governing cassava's exceptional tolerance to low N stress.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"104 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147383504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenic Lepidopteran-Pests-Resistant and Herbicide-Tolerant Cotton Through Transfer of Cry1Ab-vip3Aa and Cp4-epsps+bar Genes. 转Cry1Ab-vip3Aa和Cp4-epsps+bar基因转鳞翅目抗虫耐除草剂棉花

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-10 DOI: 10.1111/pbi.70617

Qi Mou,Jun Zhang,Zhanfeng Si,Shangkun Jin,Wanying Zhang,Tianzhen Zhang

引用次数: 0

The Advantaged Salt Inducible Suaeda salsa SsNRT2.5 and Its Promoter Significantly Enhance Nitrate Transport Efficiency and Salt Tolerance in Transgenic Arabidopsis and Rice. 盐诱变拟南芥SsNRT2.5及其启动子显著提高转基因拟南芥和水稻的硝酸盐转运效率和耐盐性。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-10 DOI: 10.1111/pbi.70600

Ranran Liu,Chenyang Li,Runtai Zhao,Congcong Song,Yan Zhou,Ruxin Zhao,Na Sui,Lei Wang,Jie Song

{"title":"The Advantaged Salt Inducible Suaeda salsa SsNRT2.5 and Its Promoter Significantly Enhance Nitrate Transport Efficiency and Salt Tolerance in Transgenic Arabidopsis and Rice.","authors":"Ranran Liu,Chenyang Li,Runtai Zhao,Congcong Song,Yan Zhou,Ruxin Zhao,Na Sui,Lei Wang,Jie Song","doi":"10.1111/pbi.70600","DOIUrl":"https://doi.org/10.1111/pbi.70600","url":null,"abstract":"Efficient nitrogen (N) uptake is critical for crop yield, but soil salinization inhibits plant nitrogen acquisition. In this study, the nitrate (NO3 -) transporter gene SsNRT2.5 and its promoter from the halophyte Suaeda salsa was investigated to elucidate the functional role in NO3 - transport under salinity and low NO3 --N conditions. SsNRT2.5 and its promoter were cloned and transformed into Arabidopsis thaliana and rice (Oryza sativa L.) for functional identification, which included analyses of expression patterns, promoter cis-element characterisation and phenotypic assessments under salt and low NO3 --N conditions. SsNRT2.5 expression was significantly upregulated under salt stress and low NO3 --N conditions in S. salsa, which improved NO3 - transport in trangenic Arabidopsis thaliana and rice. Its promoter contained salt-responsive (e.g., GT-1, DRE) and N-related (e.g., GATABOX) elements, which drove stronger salt-induced NRT2.5 expression than AtNRT2.5 promoter. Transgenic Arabidopsis and rice with SsNRT2.5 and its promoter showed enhanced NO3 - accumulation, reduced Na+ toxicity, and higher salt tolerance, as well as improved seed NO3 - storage and viability compared to WT. SsNRT2.5 plays a key role in the adaptation of S. salsa to high saline and nitrogen-limited environments, offering valuable genetic resources and theoretical insights for breeding salt-tolerant crops and developing sustainable saline agriculture.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"6 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147383505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MdRLKT1-MdRAX2-MdMKS1 Module Positively Regulating Resistance to Cytospora mali in Apple. MdRLKT1-MdRAX2-MdMKS1模块正调控苹果对马利细胞孢子虫的抗性。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-10 DOI: 10.1111/pbi.70621

Yanan Tang,Guangyao Li,Yang Li,Yuzhu Wang,Hao Feng,Lili Huang

{"title":"MdRLKT1-MdRAX2-MdMKS1 Module Positively Regulating Resistance to Cytospora mali in Apple.","authors":"Yanan Tang,Guangyao Li,Yang Li,Yuzhu Wang,Hao Feng,Lili Huang","doi":"10.1111/pbi.70621","DOIUrl":"https://doi.org/10.1111/pbi.70621","url":null,"abstract":"Valsa canker (caused by Cytospora mali = Valsa mali. C. mali) is one of the most destructive diseases affecting apple cultivation. The scarcity of natural germplasm resources with high resistance and immunity underscores the importance of exploring plant immune regulation factors of disease-resistant breeding. Protein post-translational modifications, particularly phosphorylation, are critical regulatory mechanisms in plant immunity. This study investigates how the apple receptor-like kinase MdRLKT1 modulates resistance to Valsa canker through the phosphorylation of the transcription factor MdRAX2. We found that MdRLKT1-interference (RNAi) transgenic lines exhibit increased susceptibility to C. mali infection compared to wild-type controls, indicating that MdRLKT1 positively regulates apple immune responses. Notably, MdRLKT1 interacts with the MYB transcription factor MdRAX2, facilitating its translocation into the nucleus. In vitro phosphorylation assays identified serine 147 (Ser147) as the phosphorylation site of MdRAX2 by MdRLKT1. Mutant MdRAX2S147A, with this phosphorylation site inactivated, demonstrated reduced resistance to C. mali. Further analysis revealed that MdRAX2 binds to the promoter region of MdMKS1, transcriptionally repressingits expression, whereas MdRAX2S147A failed to regulate MdMKS1 transcriptionally. Overexpression of MdMKS1 in apple resulted in reduced resistance to C. mali, suggesting that MdMKS1 negatively regulates apple immunity. These findings establish that the MdRLKT1-MdRAX2-MdMKS1 module plays a positive regulatory role in enhancing apple resistance to C. mali. In conclusion, MdRLKT1 activates the transcriptional repressor function of MdRAX2 through phosphorylation, thereby alleviating the negative regulatory effect of MdMKS1 on disease resistance and ultimately boosting the defensive capabilities of apple against pathogens.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"127 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147383506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Near Gap-Free Haplotype-Resolved Genome Assembly of Zoysia japonica Uncovers Intra-Subgenomic Gene Expression and Regulatory Variation. 结缕草近无间隙单倍型解决基因组组装揭示亚基因组内基因表达和调控变异。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-09 DOI: 10.1111/pbi.70634

Sae Hyun Lee,Preethi Purushotham,Ambika Chandra,Murukarthick Jayakodi

引用次数: 0

LsMAPK6 Phosphorylates the LsCO Protein to Enhance Its Stability and Transcriptional Activity, Promoting Floral Transition Upon High Temperatures in Lettuce LsMAPK6磷酸化LsCO蛋白，增强其稳定性和转录活性，促进生菜在高温下的成花转变

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2026-03-07 DOI: 10.1111/pbi.70630

Tingzhen Wang, Mingjia Liu, Yufeng Tian, Qingqing Tan, Jiaxuan Wang, Huifang Mu, Chaojie Liu, Huiyu Wang, Ning Liu, Yingyan Han, Jinghong Hao

{"title":"LsMAPK6 Phosphorylates the LsCO Protein to Enhance Its Stability and Transcriptional Activity, Promoting Floral Transition Upon High Temperatures in Lettuce","authors":"Tingzhen Wang, Mingjia Liu, Yufeng Tian, Qingqing Tan, Jiaxuan Wang, Huifang Mu, Chaojie Liu, Huiyu Wang, Ning Liu, Yingyan Han, Jinghong Hao","doi":"10.1111/pbi.70630","DOIUrl":"https://doi.org/10.1111/pbi.70630","url":null,"abstract":"High temperatures significantly accelerate the timing of floral transition, namely, bolting and flowering, in lettuce, which results in severe loss of marketable yield. Thus, understanding the genetic regulation of floral transition is of great interest to plant biologists and lettuce breeders. Here, we show that mitogen-activated protein kinase (LsMAPK6), whose expression and phosphorylation are stimulated by elevated temperatures, plays a positive role in the floral transition. The lsmapk6 mutants exhibit delayed bolting and flowering, whereas LsMAPK6 overexpression accelerates lettuce bolting. LsMAPK6 physically interacts with LsCO. Knockout of LsCO, to a large extent, phenocopies lsmapk6 mutants, strongly indicating that they function in the same genetic pathway. Mechanistically, LsMAPK6 phosphorylates LsCO at residue serine-258, resulting in the enhanced transcriptional activity and protein stability of LsCO and consequently the activation of LsFT-mediated flowering signalling pathways, which was confirmed by phospho-inactive and mimic analyses. Collectively, these findings reveal that the LsMAPK6-LsCO signalling module fine-tunes the timing of floral transition upon high temperatures, demonstrating that LsMAPK6 is a potential target for breeding lettuce cultivars adapted to global warming.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"127 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147368536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0