Plant Biotechnology Journal最新文献

{"title":"Analysis of BpbHLH Gene Family Responsive to MeJA Signalling in Betula platyphylla Suk. and Functional Mechanisms of BpbHLH42/44 in Genetic Improvement and Triterpenoid Biosynthesis","authors":"Ying Li, Linlin Xu, Jiale Cui, Siyao Wang, Yige Yuan, Changyixin Xiao, Xiaozhou Luo, Dmitry Baleev, Yaguang Zhan, Jing Yin","doi":"10.1111/pbi.70626","DOIUrl":"https://doi.org/10.1111/pbi.70626","url":null,"abstract":"The basic helix–loop–helix (bHLH) transcription factor family regulates plant secondary metabolism, development and stress responses. Although triterpenoids such as betulinic acid (BA), betulin (BT) and oleanolic acid (OA) from <jats:styled-content style=\"fixed-case\"> <jats:italic>Betula platyphylla</jats:italic> </jats:styled-content> Suk. are of pharmacological importance, the methyl jasmonate (MeJA)‐induced regulation of IVa bHLHs remains unclear. A total of 131 <jats:italic>BpbHLH</jats:italic> genes were identified, and functional characterisation was performed for two IVa members, <jats:italic>BpbHLH42</jats:italic> and <jats:italic>BpbHLH44</jats:italic> , in yeast, tobacco, birch cells and transgenic plants. Both enhanced triterpenoid biosynthesis but exhibited distinct specificities: <jats:italic>BpbHLH42</jats:italic> primarily promoted OA accumulation by activating <jats:italic>BpHMGR</jats:italic> , <jats:italic>BpSE1/3</jats:italic> , <jats:italic>BpW</jats:italic> and <jats:italic>BpY6</jats:italic> , while <jats:italic>BpbHLH44</jats:italic> favoured BT production through <jats:italic>BpW</jats:italic> , <jats:italic>BpY6</jats:italic> and <jats:italic>BpY11</jats:italic> . Both indirectly regulated <jats:italic>BpY9</jats:italic> via <jats:italic>BpMYB21</jats:italic> , forming a bHLH–MYB complex, and the MeJA repressor BpJAZ2 disrupted the BpbHLH42–MYB21 interaction. Transgenic birch lines further revealed divergent phenotypes: <jats:italic>BpbHLH42</jats:italic> overexpression enlarged and smoothed leaves and increased OA levels, whereas <jats:italic>BpbHLH44</jats:italic> enhanced alkali stress tolerance by elevating secondary metabolite accumulation. These results uncover the differential regulatory roles of <jats:italic>BpbHLH42</jats:italic> and <jats:italic>BpbHLH44</jats:italic> in triterpenoid biosynthesis and demonstrate their potential for metabolic engineering and genetic improvement in woody plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"11 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Transgene‐Free Multiplexed Germline Editing via Viral Delivery of an Engineered TnpB","authors":"Trevor Weiss, Maris Kamalu, Honglue Shi, Gabriel Wirnowski, Alice Ingelsson, Jasmine Amerasekera, Kamakshi Vohra, Marena I. Trinidad, Zheng Li, Emily Freitas, Noah Steinmetz, Charlie Ambrose, Kerry Chen, Jennifer A. Doudna, Steven E. Jacobsen","doi":"10.1111/pbi.70644","DOIUrl":"https://doi.org/10.1111/pbi.70644","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"27 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Conserved Magnaporthe oryzae Effector Counteracts the Rice Ubiquitin‐Proteasome System by Disrupting the E2 Function to Suppress Immunity","authors":"Min Wang, Ruyi Wang, Yehui Xiong, Xiaoman You, Fan Zhang, Xuzhao Mao, Feng He, Hui Tao, Su Jiang, Liang Fang, Xiao Xu, Jisong Wang, Zeyun Hao, Yanyan Hou, Hui Zhang, Jiyang Wang, Wenhui Zheng, Wenxian Sun, Guo‐Liang Wang, Yuese Ning","doi":"10.1111/pbi.70624","DOIUrl":"https://doi.org/10.1111/pbi.70624","url":null,"abstract":"Pathogens commonly secrete effectors into host cells to facilitate invasion. In the host ubiquitin‐proteasome system (UPS), E3 ubiquitin ligases often target pathogen effectors for degradation, thereby enhancing immune responses. In turn, pathogen effectors frequently disrupt E3 ligase function to promote virulence. However, it remains largely unclear whether pathogen effectors also interfere with other enzymes of the UPS, such as E2 ubiquitin‐conjugating enzymes. In this study, we identified a conserved effector, MoCE1, that is essential for the pathogenicity of <jats:italic>Magnaporthe oryzae</jats:italic> . MoCE1 is secreted into rice cells, where it interacts with the rice E3 ligase OsRING10 and the E2 enzyme OsUBC11. Upon <jats:styled-content style=\"fixed-case\"> <jats:italic>M. oryzae</jats:italic> </jats:styled-content> infection, OsRING10 and OsUBC11 act synergistically to degrade MoCE1 through K48‐linked polyubiquitination. Overexpression of either <jats:italic>OsRING10</jats:italic> or <jats:italic>OsUBC11</jats:italic> enhances resistance to <jats:styled-content style=\"fixed-case\"> <jats:italic>M. oryzae</jats:italic> </jats:styled-content> . To counteract this defence, MoCE1 inhibits the enzymatic activity of OsUBC11. Collectively, these findings reveal a nuanced mechanism in which a pathogen effector, regulated by a host E2–E3 pair, disrupts E2 function to escape UPS‐mediated immunity in plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"407 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Hierarchical VvbHLH30-VvERF70-VvACS2 Module Orchestrates Ethylene Biosynthesis and Cold Adaptation in Grapevine.","authors":"Yujun Hou,Darren C J Wong,Lina Wang,Yuanxi Kang,Huimin Zhou,Subash Kafle,Yalong Liu,Meilong Xu,Lin Meng,Zhenchang Liang,Guanghui Yu,Qingfeng Wang,Haiping Xin","doi":"10.1111/pbi.70640","DOIUrl":"https://doi.org/10.1111/pbi.70640","url":null,"abstract":"Ethylene is a key gaseous phytohormone that plays crucial roles in regulating plant growth, development and stress responses. However, ethylene-associated biosynthetic and transcriptional regulatory mechanisms governing cold-adaptation responses in plants remain poorly understood. In this work, genome-wide analysis from grapevines (Vitis vinifera) identified nine ACS family members, of which VvACS2, VvACS4 and VvACS6 exhibited the most dynamic transcriptional responses to cold stress and were chosen for functional validation. CRISPR-Cas9-mediated knockout and overexpression experiments revealed that VvACS2 is the major contributor to ethylene biosynthesis during cold stress in grapevine roots. Screening time-course cold treatment data from Vitis vinifera and Vitis amurensis roots identified VvERF70 and VvbHLH30 as the only two TFs, among six candidates, that directly regulate VvACS2 expression. Overexpression and CRISPR-Cas9-mediated knockout of VvERF70 or VvbHLH30 in roots further confirmed their contribution to enhanced ethylene production and cold tolerance under low-temperature treatment. Furthermore, the induction of VvACS2 was greatly enhanced when VvERF70 dimerized with VvbHLH30. Notably, VvbHLH30 further positively regulates ethylene biosynthesis under cold stress by interacting with VvERF70 and binding to its promoter. Taken together, we define a hierarchical transcriptional regulatory network where the VvbHLH30-VvERF70-VvACS2 module is pivotal for ethylene biosynthesis and underpins grapevine cold tolerance. This work provides new mechanistic insights into cold adaptation mechanisms and offers novel strategies to mitigate frost damage in agricultural crops.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"10 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147461646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Near Telomere-to-Telomere Genome of Belamcanda chinensis Provides Insights Into Genome Evolution and the Biosynthesis of Characteristic Isoflavones.","authors":"Yuan-Yuan Wang,Bi-Huan Chen,Gui-Sheng Xiang,Yi-Na Wang,Run Yang,Xiao-Bo Li,Shi-Yan Yuan,Yu-Cheng Zhao,Guang-Hui Zhang,Min-Jian Qin,Sheng-Chao Yang","doi":"10.1111/pbi.70612","DOIUrl":"https://doi.org/10.1111/pbi.70612","url":null,"abstract":"Belamcanda chinensis is a non-leguminous medicinal plant rich in bioactive isoflavones; however, the lack of a high-quality reference genome has limited elucidation of its isoflavone biosynthetic and modification network. Here, we present the first near telomere-to-telomere genome assembly of B. chinensis (4.18 Gb), generated using Illumina survey reads, PacBio HiFi and Oxford Nanopore long reads, and Hi-C scaffolding, achieving high completeness and accuracy (assembly BUSCO: 98.70%; LAI: 17.2). Ks/synteny-depth analyses and fossil-calibrated dating, with calibration at four fossil nodes, indicate two lineage-specific WGD events (~54.6 and ~27.3 MYA). These events drove significant expansions of key gene families involved in stress response and secondary metabolism. Leveraging this genome, we identified two key O-methyltransferases (BcOMT03 and BcOMT33), which are responsible for catalysing the biosynthesis of quality-marker compound irisflorentin. Meanwhile, BcUGT009, BcUGT119, BcUGT124, and BcUGT032 were characterised as glycosyltransferases with 7-O catalytic activity. Structural modelling and site-directed mutagenesis further elucidated the catalytic mechanism of BcUGT009, and its K404A mutant exhibited a significant increase in relative activity. Cross-species comparative analyses further revealed that convergent expansion of these key enzyme families underlies isoflavone biosynthetic capacity in both leguminous and non-leguminous plants. This study not only reveals the ancient polyploidization events of B. chinensis, the amplification of lineage-specific gene families and the biosynthetic pathway of characteristic isoflavones, but also provides a reference genome and functionally validated tailoring enzymes that will facilitate future heterologous pathway reconstruction and metabolic engineering of these isoflavones.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"61 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147447033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telomere to Telomere Genome Assembly and Efficient Transformation and Genome Editing in Populus euphratica.","authors":"Yi An,Rui Yang,Song Yang,Xiaohui Gou,Yuhui Li,Yan Dong,Yangyan Zhou,Xiao Han","doi":"10.1111/pbi.70609","DOIUrl":"https://doi.org/10.1111/pbi.70609","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"16 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147447034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gallic Acid-Responsive microRNAs Reprogram Lignification During Drought Acclimation Process in Spearmint.","authors":"Alessia D'Agostino,Gabriele Di Marco,Gerardo Pepe,Adelaide Teofani,Chiara Pontecorvi,Manuela Helmer-Citterich,Antonella Canini,Angelo Gismondi","doi":"10.1111/pbi.70599","DOIUrl":"https://doi.org/10.1111/pbi.70599","url":null,"abstract":"Mentha spicata L. (spearmint) is a high-value aromatic and medicinal species, whose productivity is strongly affected by water deficit. Nevertheless, the molecular mechanisms underlying drought acclimation in this mint remain largely unexplored. Thus, here, we investigated the microRNA-mediated regulatory processes triggered in M. spicata under drought stress (DS) and following treatment with gallic acid (GA), a natural phenolic compound that our research group has already documented to be a potential biostimulant for spearmint. A small-RNA sequencing approach revealed that both DS and GA induced substantial changes of the expressed miRNome, modulating 35 microRNAs (e.g., miR397a, miR159a, miR172b) whose predicted targets (e.g., Laccase-2, MYB transcription factors) are known to be involved also in lignin production. In detail, DS induced upregulation of lignin biosynthetic genes, enhancement of Laccase activity, and shifting in lignin monomer composition, promoting the putative reinforcement of the cell wall as expected during water deficiency. Conversely, GA treatment attenuated DS-induced stress, regulating microRNA-mRNA modules which balanced phytochemical and hormonal response while maintaining controlled lignification and optimising xylem function. These results highlight the pivotal role of microRNAs in orchestrating drought acclimation in M. spicata and identify GA as a compensatory agent under water-limiting conditions, capable of fine-tuning growth, cell wall remodelling, and redox homeostasis. Collectively, our findings provide molecular insights into biostimulant-mediated stress resilience and identify GA treatment as a promising biotechnological strategy to improve drought tolerance in Lamiaceae crops.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"19 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147447031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Natural LTR Retrotransposon Insertion in the Promoter of GhNAC140-Dt Boosts Cotton Lint Yield.","authors":"Yujia Yu,Xiaoguang Shang,Haitang Wang,Lijie Zhu,Xu Han,Qingfei He,Weixi Li,Yonglin Tan,Guozhong Zhu,Wangzhen Guo","doi":"10.1111/pbi.70639","DOIUrl":"https://doi.org/10.1111/pbi.70639","url":null,"abstract":"Transposable elements (TEs) are fundamental drivers of crop evolution and domestication. Whereas the underlying mechanisms of TE-mediated gene activation remain poorly understood. Lint percentage is an important yield component in cotton. Here, we report a retrotransposon insertion in the promoter of GhNAC140-Dt, a secondary wall NAC encoding gene, to promote the lint production by elevating its expression. We confirm that a 60 bp core cis-regulatory module within the TE's LTR (long terminal repeat) specifically recruits the transcription factor GhMYB46 and increases downstream genes' expression. GhNAC140-Dt overexpression activates the expressions of secondary cell wall development related genes, including GhCESA4-Dt, GhCESA4-At, and GhCOBL9-At, promotes cellulose deposition, and enhances lint percentage. This retrotransposon insertion massively emerges on the domestication transition from Gossypium hirsutum races to cultivated cotton accessions, with > 80% fixation in modern cultivars. This work deepens our understanding of TE-mediated gene activation; it also provides direct molecular evidence for \"transposon-driven yield evolution\" in crop domestication.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"32 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147439559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FvMAPK6-Mediated FvMYB44s/FvSWEET1 Dual-Layer Regulation Modulates Sugar Accumulation in Strawberry Fruit, With FvSPS3 Enabling Quality-Yield Balance.","authors":"Qianqian Feng,Lingzhi Wei,Ting Liu,Kexin Wang,Xiaojing Li,Chuang Liu,Ronghui Sun,Xia Li,Zhaonan Yin,Yanrong Wei,Huazhao Yuan,Qian Li,Bingbing Li","doi":"10.1111/pbi.70623","DOIUrl":"https://doi.org/10.1111/pbi.70623","url":null,"abstract":"Sugar content is a key determinant of fruit quality, and sugars also act as signalling molecules that regulate ripening processes, including anthocyanin accumulation. However, the molecular mechanisms underlying sugar accumulation and sugar signal-mediated ripening remain incompletely understood. In this study, we identify FvMAPK6 as an important phosphorylation hub that coordinates both sugar and anthocyanin accumulation in strawberry fruit. FvMAPK6 forms a phosphorylation cascade with FvMAPKK4, which directly phosphorylates the transcription factors FvMYB44.1 and FvMYB44.2. This phosphorylation reduces the stability and transcriptional activity of these proteins, attenuates their repression of downstream target genes such as FvCHI, FvSPS3 and FvSWEET1, thereby coordinating anthocyanin and sugar accumulation. Furthermore, FvMAPK6 increases the protein abundance of the hexose transporter FvSWEET1 in strawberry fruits and alters its transport activity through phosphorylation. We demonstrate that sucrose treatment activates FvMAPK6, reinforcing its regulation of FvMYB44s and FvSWEET1 and thus amplifying sugar and anthocyanin accumulation. These findings establish FvMAPK6 as a key regulator that integrates both sugar accumulation and signalling at both transcriptional and post-transcriptional levels. Although FvMAPK6 promotes sugar accumulation, it significantly reduces fruit yield and vegetative growth. To overcome this limitation, we screen for downstream targets of FvMAPK6 and identify FvSPS3 as a promising breeding target: modulating FvSPS3 improves fruit quality without compromising vegetative growth or yield. Collectively, our findings reveal novel regulatory pathways modulating sugar accumulation and signalling in strawberry while providing a valuable molecular target for the simultaneous improvement of fruit quality and agricultural productivity.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"26 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147439560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome-Level Genome Assembly of the Allotetraploid Gynostemma pentaphyllum Provides Novel Insights Into the Biosynthesis of Ginsenoside and Gypenoside LVI.","authors":"Peina Zhou,Si-Jie Liu,Lijin Huang,Yingping Wang,Xinyu Jiang,Wang Dong,Jianfeng Gong,Long Wang,Yuyin Zhao,Huiying Wang,Ping Li,Jia-Yu Xue,Xu Lu","doi":"10.1111/pbi.70598","DOIUrl":"https://doi.org/10.1111/pbi.70598","url":null,"abstract":"Gynostemma pentaphyllum, a herb used in tea and traditional Chinese medicine, shows geographic variation in its production of valuable dammarane-type ginsenosides and gypenoside LVI between populations from Suining (SN) and Nanning (NN). To elucidate the mechanisms underlying this differential metabolite accumulation, a chromosome-level genome for G. pentaphyllum (SN population) was assembled. The analysis revealed that SN is a tetraploid (~1.2 Gb), resulting from a recent whole-genome duplication event in a diploid ancestor. Phylogenetic analysis indicates SN and diploid NN share a recent common ancestor, diverging approximately 4.95 million years ago. Chromosome evolution analysis confirmed SN is an allotetraploid with clear subgenomic differentiation. This genome, combined with multi-omics data, enabled the screening of candidate P450 genes involved in ginsenoside/gypenoside LVI biosynthesis. In vivo and in vitro experiments confirmed that GpCYP88AB3 functions as a bifunctional enzyme by first hydroxylating dammarenediol-II at C-12 to yield protopanaxadiol (PPD), and then hydroxylating PPD at C-2 to form 2α-OH-PPD. Phylogenetically, GpCYP88AB3 and similar enzymes from Araliaceae belong to distinct CYP subfamilies, demonstrating convergent evolution of this function between the two plant families and highlighting the functional plasticity of P450s. Evolutionary analysis suggests that GpCYP88AB3 emerged from a CYP88 gene family expansion in the tetraploid G. pentaphyllum. This expansion occurred after, but was not directly caused by, the whole-genome duplication event. This study elucidates the biosynthetic pathway for the key metabolites in G. pentaphyllum, providing a foundation for future metabolic engineering and synthetic biology applications.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"8 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147439561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}