Plant Biotechnology Journal最新文献

{"title":"Metabolomic Analysis Reveals Structural Diversity of Pectic Polysaccharides From Prunus persica (L.) Batsch and Their Relationship With Purees Characteristics.","authors":"Meng Liu,Xuan Liu,Shaojie Fu,Qianhui Yang,Christophe Blecker,Jinfeng Bi","doi":"10.1111/pbi.70709","DOIUrl":"https://doi.org/10.1111/pbi.70709","url":null,"abstract":"To clarify how pectin heterogeneity relates to puree quality, this study investigated associations among pectin fractions, metabolite profiles, and physicochemical properties of peach purees from 20 cultivars with distinct viscosity, turbidity, and colour. Metabolomic analysis showed that sugars, organic acids, and phenolic compounds were closely associated with the yield of chelator-soluble pectin (CSP), indicating coordinated variation between metabolite profiles and structurally integrated pectin fractions across cultivars. CSP yield was further related to resistance to deformation, as reflected by yield stress. In contrast, rheological behaviour after yielding was more strongly associated with water-soluble pectin (WSP), whose yield and structural features were linked to viscosity, viscoelastic balance, and flow behaviour. Phenolic compounds such as taxifolin and phlorizin chalcone were associated with puree colour. Collectively, these results establish an applied evaluation framework linking metabolite variation, pectin fraction distribution, and cultivar-dependent quality traits. By connecting micro-biochemical compositions (metabolite profiles and pectin fractions) with macroscopic processing-related properties, this framework provides insights into cell wall-related characteristics relevant to peach puree quality and processing performance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"79 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148400715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Modulation of Eukaryotic Release Factor 1 (OseRF1) by an RNA Aptamer Enhances Drought Adaptation in Rice.","authors":"Shuangfeng Dai,Mingming Chen,Haomin Chen,Yuantao Ye,Hong Huang,Renrong Zhang,Zhenyu Guo,Yongxiang Huang","doi":"10.1111/pbi.70720","DOIUrl":"https://doi.org/10.1111/pbi.70720","url":null,"abstract":"Drought stress severely restricts rice productivity, necessitating strategies to enhance stress resilience. Here, we identify translation termination as a tunable regulatory node for stress adaptation. Using 25 rounds of SELEX, we isolated RTAR, an RNA aptamer that binds the rice eukaryotic release factor 1 (OseRF1) with nanomolar affinity. Structural and functional analyses revealed that RTAR targets the OseRF1 N-domain through a conserved pocket centered on Arg46, inhibiting stop-codon recognition and promoting translational readthrough in rice cell extracts. Under drought stress, RTAR co-localized with OseRF1 in rice protoplasts and accelerated its depletion, thereby reprogramming termination fidelity. Ribosome profiling (Ribo-seq) identified candidate genes, including OsDR8, with increased ribosome occupancy downstream of annotated stop codons, indicating a transcriptome-wide effect on translation termination. RTAR expression significantly enhanced drought tolerance in rice seedlings, as evidenced by increased survival, chlorophyll retention and reduced membrane injury. These findings were further validated in stable transgenic lines subjected to soil-based drought stress, where RTAR-expressing plants exhibited enhanced whole-plant drought resilience. This phenotype was accompanied by upregulation of drought-responsive genes and was abolished in OseRF1-R46A mutants, demonstrating the functional importance of the Arg46-centered binding pocket. Furthermore, GFP-tagging assays revealed higher-molecular-weight OsDR8 isoforms consistent with C-terminal extension, providing direct evidence of translational readthrough in vivo. Together, these results establish RTAR as a precise and reversible modulator of translation termination and identify OseRF1 as a promising target for engineering drought adaptation in crops.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"63 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148400713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Functional Trastuzumab in Phaeodactylum tricornutum: Advancing Microalgae as Next-Generation Sustainable Antibody Biofactories.","authors":"Charlotte Toustou,Manon Salvetti,Magalie Bénard,Christelle Fécourt,Sébastien Calbo,Kylian Jouie,France Blanchard,Camille N'Zué,Chloé Placines,Thomas Badou,Nicolas Piton,Patrice Lerouge,Ludovic Galas,Muriel Bardor","doi":"10.1111/pbi.70710","DOIUrl":"https://doi.org/10.1111/pbi.70710","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"34 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148388536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Fusion Between Common Bean Phaseolin and the N-Terminal Domain of Maize 16 kDa Gamma Zein: Clues for Designing Nutritionally Improved Seed Storage Proteins.","authors":"Emma Costa,Lucrezia Luciani,Mahsa Taghipour Rahimi,Alessandro Vitale,Emanuela Pedrazzini","doi":"10.1111/pbi.70719","DOIUrl":"https://doi.org/10.1111/pbi.70719","url":null,"abstract":"Increasing the nutritional value of seed storage proteins (SSPs) has been largely hindered by limited knowledge of how the features of the various SSP domains determine high accumulation in protein storage vacuoles (PSV) or as endoplasmic reticulum (ER)-located insoluble protein bodies (PB). We had previously designed zeolin, a fusion between phaseolin (PHSL, the main bean SSP, a soluble trimeric protein located in PSV) and the N-terminal domain of 27 kDa γ-zein (27γz, a major maize PB prolamin). Zeolin has a more balanced essential amino acid composition than each parental sequence and forms insoluble homomeric PB very similar to natural maize heteropolymeric PB, indicating that the zein domain has a dominant effect over PHSL. We had also determined that the paralog 16 kDa γ-zein (16γz) remains partially soluble and forms unusual threads in the ER when ectopically expressed. We now produced zeolin2 (zeo2), by fusing PHSL to the N-terminal domain of 16γz. We show that only a minor proportion of zeo2 remains in the ER, without forming PBs or threads. Zeo2 mainly forms soluble trimers with biochemical characteristics very similar to PHSL trimers, and traffics to the vacuole via the Golgi complex. This indicates that the 16γz domain fails to significantly dominate over PHSL. In transgenic arabidopsis, zeo2 accumulates to markedly higher levels than phaseolin and only slightly lower levels than zeolin. Zeo2 is also more accessible than zeolin to proteases during the early stages of seed germination. These findings provide new information relevant for the design of modified, nutritionally enhanced storage proteins.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"144 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148388575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights Into the Origin and Local Adaptation Evolution of the Cultivated Sesame With Telomere‐to‐Telomere High‐Quality Genome","authors":"Weifei Yang, Hengchun Cao, Hui Guo, Hongmei Miao, Guiting Li, Yinghui Duan, Ming Ju, Qiuzhen Tian, Qin Ma, Pengjie Chang, Zhanyou Zhang, Cong Mu, Xiaoxu Feng, Libin Wei, Weixiu Hou, Andrew H. Paterson, Haiyang Zhang","doi":"10.1111/pbi.70714","DOIUrl":"https://doi.org/10.1111/pbi.70714","url":null,"abstract":"Sesame ( <jats:styled-content style=\"fixed-case\"> <jats:italic>Sesamum indicum</jats:italic> </jats:styled-content> L., 2 <jats:italic>n</jats:italic> = 26) is one of the oldest oilseed crops and is often called the ‘queen of oilseeds’ due to its high content of unsaturated fatty acids and natural antioxidants. Despite its long history, the origin and global spread of cultivated sesame remain unresolved. We assembled a telomere‐to‐telomere (T2T), high‐quality reference genome of sesame (cv. Yuzhi11) to investigate sequence differences between genomes and its origin and the local adaptation evolution of flowering time (DF). We generated a 305 Mb T2T sesame reference genome (cv. Yuzhi11) with &gt; 99.99% base‐level accuracy, identifying 31 063 protein‐coding genes. Repetitive elements accounted for 52.03% of the genome. Population genomic analysis of 927 accessions from 14 regions identified four major groups. Integrative analyses of linkage disequilibrium decay (LD), nucleotide diversity (π), and fixation index ( <jats:italic>F</jats:italic> <jats:sub>ST</jats:sub> ) support East Africa as the center of origin, with subsequent migration through the Middle East, to South Asia, South‐East Asia, East Asia and ultimately to other parts of the world. Genome‐wide association studies (GWAS) and selection scans identified 30 genes associated with flowering time. <jats:italic>SiUBP16</jats:italic> is a candidate associated with 7.6% of DF variation. Early‐flowering accessions carried up to 225 favourable alleles. A flowering time prediction model for high‐latitude regions achieved 96% accuracy. We present a high‐quality T2T reference genome for cultivated sesame, shedding light on its origin, evolutionary history, and regional flowering time adaptation. This genome insights valuable tools for breeding programs aimed at improving yield and environmental adaptation in sesame and related crops.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"33 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148379893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Robust Framework for Maize Elite Line Genome Editing Through Enhanced HI ‐Edit via LbCas12a Activity Optimization","authors":"Dawei Liang, Huanhuan Guo, Juan Wei, Fugui Zhu, Yuguo Zhang, Julie Green, Yun Ji, Huaibing Jin, Xiujuan Zhang, Huaping Gui, Hongmei Dan, Yubo Liu, Yu Zhang, Han Wang, Yutong Jiang, Lizhao Geng, Jian Lv, Wen Cai, Weibin Song, Timothy Kelliher, Xi Chen, Rachel Egger","doi":"10.1111/pbi.70715","DOIUrl":"https://doi.org/10.1111/pbi.70715","url":null,"abstract":"Haploid induction coupled with genome editing (HI‐Edit) enables direct modification of commercial crop varieties, bypassing the need for trait introgression or direct transformation of elite lines with CRISPR machinery. However, its widespread application has been constrained by low haploid editing rates (HER), the proportion of haploids carrying edits within the short window between double fertilization and uniparental chromosome elimination. Here, we report substantial improvements in maize HI‐Edit efficiency through three complementary strategies: (1) driving an optimized LbCas12a variant (LbCas12aV) using promoters that are highly active in sperm cells and early zygotes; (2) applying a post‐pollination heat treatment; and (3) fusing LbCas12aV with the UBA2 domain (ubiquitin‐associated domain‐2 of <jats:styled-content style=\"fixed-case\"> <jats:italic>Arabidopsis thaliana</jats:italic> </jats:styled-content> RAD23) to enhance protein stability during haploid induction. Post‐pollination heat treatment alone increased HER to 19.1% (up to 12‐fold improvement depending on the target site), providing a simple and effective method to boost the yield of edited doubled haploid (DH) plants. UBA2 fusion improved HER by 6‐fold at the <jats:italic>Waxy1</jats:italic> ( <jats:italic>Wx1</jats:italic> ) locus and 4.5‐fold at the <jats:italic>Glossy2</jats:italic> ( <jats:italic>Gl2</jats:italic> ) locus under normal conditions. Strikingly, combining UBA2 fusion with heat treatment raised the average HER to 25% across multiple events targeting <jats:italic>Wx1</jats:italic> , with the highest HER reaching 33%. Collectively, these findings demonstrate that increasing CRISPR‐Cas protein abundance and modulating environmental conditions can overcome key bottlenecks in HI‐Edit. We establish a robust, scalable framework that is readily transferable to other crops for elite‐line genome editing.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"78 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148379936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR /Cas9‐Mediated Editing of Four Ca 2+ ‐Binding Receptor‐Like Cytoplasmic Kinases Improves Soybean Seed Size and Yield at Dense Planting","authors":"Wanhong Li, Jun Jin, Tianyue Gu, Xiaoxi Cai, Bowei Jia, Yan Wang, Dezhi Han, Xiaofei Yan, Xiping Hu, Xiaoli Sun, Sijia Lu, Mingzhe Sun","doi":"10.1111/pbi.70713","DOIUrl":"https://doi.org/10.1111/pbi.70713","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"78 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148379737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of In Vitro and In Planta Strategies for Efficient Production of High‐Value Steviol Glycosides","authors":"Sing Hui Leong, Benny Jian Rong Sng, Ian Kin Yuen Choi, In‐Cheol Jang","doi":"10.1111/pbi.70718","DOIUrl":"https://doi.org/10.1111/pbi.70718","url":null,"abstract":"Steviol glycosides (SGs) are a group of sweet‐tasting diterpene glycosides found in <jats:styled-content style=\"fixed-case\"> <jats:italic>Stevia rebaudiana</jats:italic> </jats:styled-content> . Production of high‐value SGs, Rebaudioside D (Reb D) and Reb M, is limited by inefficient conversion of Reb A to Reb D and stevioside to Reb E. To address this, we investigated the combined use of stevia UDP‐glycosyltransferase SrUGT76G1 and a UDP‐glycosyltransferase from <jats:styled-content style=\"fixed-case\"> <jats:italic>Setaria italica</jats:italic> </jats:styled-content> (SiUGT) to enhance SG biosynthesis. SiUGT was previously shown to efficiently convert Reb A and stevioside to Reb D and Reb E, respectively, in yeast. Using an in vitro enzymatic assay, we demonstrated that combining SrUGT76G1 and SiUGT significantly improved conversion efficiency, with stevioside identified as a more favourable starting substrate due to reduced formation of unwanted byproducts. Subsequently, SrUGT76G1 and SiUGT were expressed individually and as a fusion protein in the heterologous host <jats:italic>Nicotiana benthamiana</jats:italic> via transient and stable transformation. While transient expression of either SrUGT76G1 or SiUGT in <jats:italic>N. benthamiana</jats:italic> resulted primarily in intermediate SGs being produced, transient expression of the SrUGT76G1‐SiUGT fusion protein enabled production of both Reb D and Reb M upon substrate feeding. Likewise, stable transgenic <jats:italic>N. benthamiana</jats:italic> lines expressing the fusion construct <jats:italic>SrUGT76G1‐SiUGT</jats:italic> accumulated Reb D and Reb M, with production levels correlating positively with the transgene expression. Overall, this study established an optimised in vitro enzymatic system for efficient Reb M production, as well as demonstrated the feasibility of <jats:italic>N. benthamiana</jats:italic> as an in planta platform for heterologous SGs biosynthesis, providing novel strategies with potential applicability for industrial production of high‐value SGs.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"32 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant‐Based Production of Human Major Histocompatibility Complex Class II Molecules","authors":"Abdelaziz Ramadan, Hiroko Miyadera, Kaho Oka, Kohei Ishikawa, Toshiya Kobayashi, Misaki Kobayashi, Mitsunori Shiroishi, Emiko Noguchi, Kenji Miura","doi":"10.1111/pbi.70705","DOIUrl":"https://doi.org/10.1111/pbi.70705","url":null,"abstract":"Major histocompatibility complex class II (MHC II) protein triggers adaptive immune responses by presenting antigens on antigen‐presenting cells (APCs) to be recognised by CD4+ T cells. Recombinant MHC II proteins are effective for the analysis of peptide‐binding interaction, detection of antigen‐specific T‐cells and clinical testing for donor‐specific anti‐human leukocyte antigen (HLA) antibody for organ transplantation. However, <jats:styled-content style=\"fixed-case\"> <jats:italic>Escherichia coli</jats:italic> </jats:styled-content> ‐based production is limited due to difficulty in obtaining properly folded protein complexes. In this study, we utilised the expression machinery of <jats:italic>Nicotiana benthamiana</jats:italic> via the Tsukuba System to produce the soluble extracellular domains of MHC II proteins. Thirteen different MHC II proteins of the three isotypes (HLA‐DR, DQ and DP) conjugated with peptides (pMHC II) were successfully expressed. The HLA‐DRA*01:01/DRB1*01:01 covalently linked to a class II‐associated invariant chain peptide (CLIP) or influenza hemagglutinin (HA, 306–318) was further analysed as a model. The resultant proteins were detectable in soluble fractions and purified using metal affinity purification and ion exchange chromatography, yielding 4.37 and 2.81 μg/gFW, respectively. pMHC II were also biotinylated in vitro using commercial BirA, enabling future construction of tetramers or multimers. The purified proteins were confirmed through enzyme‐linked immunosorbent assay (ELISA) using anti‐HLA antibodies. Furthermore, Surface plasmon resonance biosensor (Biacore) verified glycosylation‐dependent recognition of MHC II‐HA complex by cognate T‐cell receptor (HA1.7 TCR), resulting in an equilibrium dissociation constant of K <jats:sub>D</jats:sub> = 3.62 ± 1.59 μM. Overall, this plant‐based system offers a promising scalable and economical alternative for large‐scale production of functional MHC II proteins for basic and clinical applications.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"35 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of an Agrobacterium-mediated CRISPR/Cas9 Genome Editing System for Kenaf (Hibiscus cannabinus).","authors":"Xueqing Pan, Lingling Zhuang, Siyan Wu, Chuanyu Wang, Qin Li, Jianmin Qi, Pingping Fang, Jiantang Xu, Aifen Tao, Shuangxia Jin, Liwu Zhang","doi":"10.1111/pbi.70657","DOIUrl":"10.1111/pbi.70657","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"4719-4721"},"PeriodicalIF":12.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13278532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}