Plant Biotechnology Journal最新文献

{"title":"Correction to ‘The genome of Cymbidium sinense revealed the evolution of orchid traits’","authors":"","doi":"10.1111/pbi.70727","DOIUrl":"10.1111/pbi.70727","url":null,"abstract":"<p>\u0000 <span>Yang, F.-X.</span>, <span>J. Gao</span>, <span>Y.-L. Wei</span>, et al. <span>2021</span>. “ <span>The Genome of <i>Cymbidium sinense</i> Revealed the Evolution of Orchid Traits</span>.” <i>Plant Biotechnology Journal</i> <span>19</span>: <span>2501</span>–<span>2516</span>. https://doi.org/10.1111/pbi.13676.\u0000 </p><p>In the above article, the authors would like to update Figure 4b. The ultrastructure images of flower bud developmental stage 0 and stage 1 in the second row, which corresponded to plant morphology of stage 0 and stage 1 in the first row, were mistakenly displayed. The correct figure is shown below.</p><p>We apologize for these errors.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5239-5240"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70727","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148451770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Satellite DNA Editing Enables Multiplexed Chromosome Restructuring in Populus.","authors":"Ran Zhou, Margot S S Chen, MaKenzie R Drowns, Kathrine Mailloux, Kangquan Yin, Brieanne Vaillancourt, C Robin Buell, Chung-Jui Tsai","doi":"10.1111/pbi.70741","DOIUrl":"10.1111/pbi.70741","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148807840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplexed Engineering of Disease Resistance and Lodging Tolerance in Wheat Via a Single Xylan Modifying Glycosyltransferase.","authors":"Shufang Sun, Chunhui Li, Zehua Wang, Chunyang Ma, Xinhui Niu, Qunqing Wang, Qian Xu","doi":"10.1111/pbi.70747","DOIUrl":"10.1111/pbi.70747","url":null,"abstract":"<p><p>Breeding crops with robust disease resistance often compromises yield, a persistent challenge in agricultural improvement. We report that modifying cell wall architecture through a xylan-specific glycosyltransferase successfully breaks this trade-off in wheat. TaXAX1, a pathogen-induced member of the glycosyltransferase (GT)61 family, catalyses arabinose modification of xylan and is predominantly localized to the Golgi apparatus. CRISPR/Cas9 knockout mutants of Taxax1 exhibited enhanced susceptibility to three major fungal diseases: Fusarium head blight (FHB), Fusarium crown rot (FCR) and stripe rust. Conversely, overexpression of TaXAX1 significantly enhanced resistance to all three pathogens concurrently. Remarkably, this multi-disease resistance was achieved without any reduction in grain yield. Mechanistically, TaXAX1 mediates cell wall fortification by increasing xylan arabinose modification arabinosylation, upregulating the phenylpropanoid pathway and enhancing the deposition of lignin, cellulose and phenolic cross-linkers. This resulted in a thicker and more reinforced secondary cell wall, which also translated into superior stem strength. Our study identifies TaXAX1 as a master regulator of cell wall remodelling and provides a compelling strategy for designing wheat varieties with integrated resilience against multiple diseases and environmental stresses, while safeguarding yield potential.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148807903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Cell Transcriptomics Reveals the FA9-VAP Module Regulating Fatty Acid Accumulation in Soybean Seeds.","authors":"Hui Li, Tianshu Li, Xiaorui Xu, Jinhang Cui, Xin Chen, Jingyi Huang, Jia Song, Cui Mu, Xinyu Hong, Chunyan Liu, Ning Wang, Xue Han, Sui Wang, Qingshan Chen, Zhaoming Qi","doi":"10.1111/pbi.70750","DOIUrl":"10.1111/pbi.70750","url":null,"abstract":"<p><p>As a major commercial legume crop, soybean ranks among the world's most significant sources of edible oil and plant protein. We previously identified a SEIPIN homologue (FA9) at the fatty acid 9 locus that promotes fatty acid accumulation in soybean. To examine the detailed molecular mechanisms by which FA9 regulates lipid metabolism, we performed single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (stRNA-seq) of wild-type and FA9-knockout soybean seeds at the late maturity stage. scRNA-seq analysis identified 26 transcriptional clusters and revealed the spatial distribution of FA9 in seeds, in which the deletion of FA9 altered lipid and storage-related transcriptional programmes. On the basis of single-cell sequencing and immunoprecipitation-mass spectrometry (IP-MS), the vesicle-associated membrane protein (VAMP)-associated protein (VAP) was identified, and subsequent experiments demonstrated that FA9 interacts specifically with VAP via its N-terminal FFAT motif at the endoplasmic reticulum. Seeds of vap knockout (vap-KO1 and vap-KO2) and fa9 vap double knockout (fa9 vap-KO) lines, created by CRISPR-Cas9 gene editing, had higher protein contents and lower total fatty acid contents than wild-type soybean, whereas overexpression of FA9 and VAP enhanced lipid droplet formation in Nicotiana benthamiana. These findings reveal that FA9 interacts with VAP to promote lipid droplet biogenesis and lipid transport, thereby driving fatty acid accumulation in soybean seeds. This research provides new insight into the molecular mechanisms that regulate seed oil synthesis and identifies potential target genes for improvement of soybean oil quality through molecular breeding.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148807897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A MYB ‐Related Transcriptional Module Cooperatively Represses Xylem Development in Poplar","authors":"Gaofeng Fan, Jiahui Jiang, Zihan Cheng, Feixiang Ma, Zhanling Sun, Zelin Li, Yu Qi, Guanzheng Qu, Tingbo Jiang","doi":"10.1111/pbi.70745","DOIUrl":"https://doi.org/10.1111/pbi.70745","url":null,"abstract":"MYB transcription factors play crucial roles in regulating plant growth and development, but the functions of the MYB‐related subfamily in woody plants remain poorly understood. In this study, we characterized <jats:italic>PagMYBR028</jats:italic> , an MYB‐related transcription factor from poplar ‘84 K’ ( <jats:styled-content style=\"fixed-case\"> <jats:italic>Populus alba</jats:italic> </jats:styled-content> × <jats:italic>Populus glandulosa</jats:italic> ), which was predominantly expressed in leaves and xylem. Compared with WT plants, <jats:italic>PagMYBR028</jats:italic> ‐overexpressing transgenic poplar exhibited reduced leaf area and retarded secondary xylem development, whereas <jats:italic>PagMYBR028</jats:italic> ‐RNAi lines displayed the opposite phenotypes. Further analysis revealed that PagMYBR028 interacts with PagMYBR005, a related regulator with overlapping functions in poplar development. Both proteins directly repress the expression of <jats:italic>PagGRF12b</jats:italic> , and their interaction enhances this transcriptional repression. In addition, overexpression of <jats:italic>PagGRF12b</jats:italic> promoted secondary cell wall deposition. Taken together, our results delineate a PagMYBR028‐PagMYBR005‐PagGRF12b module that coordinately regulates poplar growth and secondary wall development, providing new insights into the transcriptional network underlying wood formation and a theoretical basis for molecular breeding in trees.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"22 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148768156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Overexpression of FERULOYL‐CoA 6′‐HYDROXYLASE 1 and COUMARIN SYNTHASE Leads to Coumarin‐Enriched Lignin and Improved Saccharification in Greenhouse‐ and Field‐Grown Poplar","authors":"Nette De Ridder, Lennart Hoengenaert, Jan Van Doorsselaere, Wim De Clercq, Geert Goeminne, Sarah Liu, Fachuang Lu, John Ralph, Ruben Vanholme, Wout Boerjan","doi":"10.1111/pbi.70739","DOIUrl":"https://doi.org/10.1111/pbi.70739","url":null,"abstract":"The urgent need for renewable resources has increased the interest in woody biomass to manufacture bio‐based products. However, lignin recalcitrance limits the enzymatic conversion of wood into fermentable sugars, posing a major challenge for biomass deconstruction. To address this problem, we aimed at incorporating the coumarin scopoletin into the lignin polymer of poplar ( <jats:italic> <jats:styled-content style=\"fixed-case\">Populus tremula</jats:styled-content> × <jats:styled-content style=\"fixed-case\">P</jats:styled-content> </jats:italic> <jats:styled-content style=\"fixed-case\"> <jats:italic>. alba</jats:italic> </jats:styled-content> ) by expressing <jats:italic>FERULOYL‐CoA 6′‐HYDROXYLASE 1</jats:italic> ( <jats:italic>F6′H1</jats:italic> ) and <jats:italic>COUMARIN SYNTHASE</jats:italic> ( <jats:italic>COSY</jats:italic> ) in lignifying cells. Three constructs were evaluated: two bicistronic constructs, SCOP1 ( <jats:italic>COSY</jats:italic> followed by <jats:italic>F6′H1</jats:italic> ) and SCOP2 ( <jats:italic>F6′H1</jats:italic> followed by <jats:italic>COSY</jats:italic> ), and one monocistronic, SCOP3 (only <jats:italic>F6′H1</jats:italic> ). SCOP1 poplars produced most free scopoletin without altering overall lignin, cellulose or hemicellulose content. SCOP2 poplars were overall less efficient in scopoletin production and most of these lines showed a severe biomass yield penalty, whereas SCOP3 caused plant lethality. NMR and metabolic analyses confirmed that scopoletin cross‐coupled with G and S monomers during lignification in SCOP1 lines. In addition to scopoletin, the detection of benzodioxane structures revealed the incorporation of dihydroxycoumarins. Overall coumarin incorporation in lignin amounted up to 2.3%. After alkaline pretreatment, wood from greenhouse‐grown SCOP1 poplars released up to 29% more glucose compared to the wild type upon limited saccharification. Field‐testing of three SCOP1 lines showed a 6 to 11% increase in saccharification efficiency, with the line containing the lowest scopoletin levels maintaining normal growth. These results demonstrate that engineering lignin composition in poplar can improve saccharification, and emphasize the importance of construct design, translational research and field validation.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"35 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148754467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unexpected Suppression of Tomato Fruit Cuticle Formation by Overexpression of a Key Cutin Biosynthetic Enzyme","authors":"Gulab Chand Arya, Ekram Wassel, Vibha Mishra, Ekaterina Manasherova, Ruth E. Stark, Hagai Cohen","doi":"10.1111/pbi.70742","DOIUrl":"https://doi.org/10.1111/pbi.70742","url":null,"abstract":"Fruit cuticles regulate the diffusion of water, gases and solutes, functioning as essential protective interfaces against environmental and biotic stresses. Engineering cuticle structure and mechanics therefore represents a promising strategy for improving fruit quality and resilience. To enhance biosynthesis of cutin, the major structural component of the cuticle, we generated transgenic tomato lines expressing the <jats:styled-content style=\"fixed-case\"> <jats:italic>Arabidopsis thaliana</jats:italic> </jats:styled-content> <jats:italic>GLYCEROL‐3‐PHOSPHATE sn‐2‐ACYLTRANSFERASE 4</jats:italic> ( <jats:italic>AtGPAT4</jats:italic> ) gene specifically in exocarp tissues. AtGPAT4 is a key enzyme providing acylglycerol intermediates for cutin polymer assembly. We performed comprehensive phenotypic, microscopic, spectroscopic, transcriptional and surface nanomechanical analyses to monitor the structural consequences of <jats:italic>AtGPAT4</jats:italic> overexpression and evaluated functional traits including water loss and susceptibility to the <jats:italic>Botrytis cinerea</jats:italic> fungus. Contrary to expectations, exocarp‐targeted expression of <jats:italic>AtGPAT4</jats:italic> generated fruits with cuticles that had diminished levels of cutin and epicuticular waxes, together with decreased cuticular stiffness and resistance to deformation, and whole fruit firmness. Evidently, these structural and chemical alterations did not affect thermal stability or water retention, but they increased susceptibility to fungal infection by <jats:styled-content style=\"fixed-case\"> <jats:italic>B. cinerea</jats:italic> </jats:styled-content> . Although the engineering outcomes were unintended, they offered mechanistic insights into how manipulation of cutin biosynthesis pathways reshapes cuticle architecture, polymer composition and nanomechanical performance. This work underscores the complexity of engineering fruit surface traits and offers new perspectives for future biotechnological strategies aimed at improving cuticle robustness and pathogen resistance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"56 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148754468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unexpected Suppression of Tomato Fruit Cuticle Formation by Overexpression of a Key Cutin Biosynthetic Enzyme.","authors":"Gulab Chand Arya, Ekram Wassel, Vibha Mishra, Ekaterina Manasherova, Ruth E Stark, Hagai Cohen","doi":"10.1111/pbi.70742","DOIUrl":"https://doi.org/10.1111/pbi.70742","url":null,"abstract":"<p><p>Fruit cuticles regulate the diffusion of water, gases and solutes, functioning as essential protective interfaces against environmental and biotic stresses. Engineering cuticle structure and mechanics therefore represents a promising strategy for improving fruit quality and resilience. To enhance biosynthesis of cutin, the major structural component of the cuticle, we generated transgenic tomato lines expressing the Arabidopsis thaliana GLYCEROL-3-PHOSPHATE sn-2-ACYLTRANSFERASE 4 (AtGPAT4) gene specifically in exocarp tissues. AtGPAT4 is a key enzyme providing acylglycerol intermediates for cutin polymer assembly. We performed comprehensive phenotypic, microscopic, spectroscopic, transcriptional and surface nanomechanical analyses to monitor the structural consequences of AtGPAT4 overexpression and evaluated functional traits including water loss and susceptibility to the Botrytis cinerea fungus. Contrary to expectations, exocarp-targeted expression of AtGPAT4 generated fruits with cuticles that had diminished levels of cutin and epicuticular waxes, together with decreased cuticular stiffness and resistance to deformation, and whole fruit firmness. Evidently, these structural and chemical alterations did not affect thermal stability or water retention, but they increased susceptibility to fungal infection by B. cinerea. Although the engineering outcomes were unintended, they offered mechanistic insights into how manipulation of cutin biosynthesis pathways reshapes cuticle architecture, polymer composition and nanomechanical performance. This work underscores the complexity of engineering fruit surface traits and offers new perspectives for future biotechnological strategies aimed at improving cuticle robustness and pathogen resistance.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Rewiring of the Shikimate Pathway Enables Rose‐Scented Rice","authors":"Xiaoqing Cui, Wenhao Yao, Honglin Yang, Yangning Ou, Keyi Chen, Yifei Qin, Chengxuan Li, Boxi Zhu, Wenyi Wang, Jiayang Li, Mengyun Xu, Jian Wu","doi":"10.1111/pbi.70743","DOIUrl":"https://doi.org/10.1111/pbi.70743","url":null,"abstract":"Rice fragrance is a key determinant of grain quality, yet the aroma profile of cultivated rice is highly uniform and largely dominated by the popcorn‐like compound 2‐acetyl‐1‐pyrroline. Here we expanded the aromatic repertoire of rice by engineering a rose‐like fragrance through reconstruction of the phenylalanine‐derived 2‐phenylethanol (2‐PE) pathway in the endosperm. To increase phenylalanine precursor supply, we co‐expressed an engineered G211R/G212S variant of the rice 3‐deoxy‐D‐arabino‐heptulosonate 7‐phosphate synthase isoform OsDHS2 ( <jats:italic>LOC_Os08g37790</jats:italic> ; mOsDHS2) with heterologous phenylacetaldehyde synthase (PAAS) and phenylacetaldehyde reductase (PAR) in the endosperm. As a result, the engineered grains accumulated up to 2014 μg/kg 2‐PE, conferring a distinct rose‐like aroma. Unexpectedly, metabolic rewiring also reshaped grain composition, resulting in nearly doubled soluble protein content and increased levels of several vitamin B6‐related metabolites, including the active coenzyme forms pyridoxal 5′‐phosphate and pyridoxamine 5′‐phosphate. These findings reveal extensive associated metabolic coordination between aromatic amino acid pathways and central seed metabolism. Our work establishes a strategy for creating designer fragrances in staple crops while simultaneously improving nutritional traits, providing a versatile platform for producing high‐value metabolites in cereal grains.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"56 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148726421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Planta Expression of Thermophilic GtGBE Mimics BEIIb and Modifies Rice Starch Structure Without Yield Penalties","authors":"Feifei Xu, Jianming Pan, Meng Li, Xinyu Li, Jiayuan Chang, Wenjia Fu, Kaiwen Gu, Jinsong Bao","doi":"10.1111/pbi.70744","DOIUrl":"https://doi.org/10.1111/pbi.70744","url":null,"abstract":"Enzymatic modification using glycosyltransferases offers a green route to produce highly branched starch to enhance starch functionality in the food industry, yet this approach faces challenges including the high cost of enzyme production and inefficiency. Here, we developed a viable <jats:italic>in planta</jats:italic> strategy by heterologously expressing an α‐glucan branching enzyme (GBE) from <jats:italic>Geobacillus thermoglucosidans</jats:italic> STB02 (GtGBE) in rice, aiming to tailor starch branching structures directly in a living plant system. Several independent transgenic lines were generated, expressing the <jats:italic>GtGBE</jats:italic> gene under the control of either the endosperm‐specific rice <jats:italic>starch branching enzyme IIb</jats:italic> ( <jats:italic>BEIIb</jats:italic> ) promoter (pBEIIb) or the constitutive maize <jats:italic>Ubiquitin</jats:italic> promoter (pUbi). GtGBE generated distinct promoter‐dependent branching patterns. The pBEIIb‐driven construct partially recapitulated BEI‐deficient‐like profiles through the combined effect of downregulated endogenous <jats:italic>BEI</jats:italic> and relatively low GtGBE expression levels. In contrast, the pUbi‐driven construct enhanced BEIIb‐like activity through sustained high GtGBE accumulation and upregulation of endogenous <jats:italic>BEIIb</jats:italic> . Both strategies increased short amylopectin chains at the expense of intermediate/long chains and amylose, resulting in decreased relative crystallinity, apparent amylose content, gelatinization temperatures, and peak viscosity, alongside improved retrogradation resistance and rheological properties. Notably, GtGBE expression driven by the <jats:italic>BEIIb</jats:italic> promoter showed greater stability under heat stress. Moreover, pUbi‐GtGBE transgenic lines exhibited a significant increase in 1000‐grain weight and filled grain number per panicle without compromising other agronomic traits, highlighting the dual potential of this strategy for agricultural and industrial applications. This work establishes GtGBE as an effective molecular tool for the direct and tailored bioengineering of starch in a living plant system.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"137 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148715873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}