Taiyu Chen, Marta Hojka, Philip Davey, Yaqi Sun, Fei Zhou, Tracy Lawson, Peter J. Nixon, Yongjun Lin, Lu‐Ning Liu
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引用次数: 0
Abstract
SummaryAlthough Rubisco is the most abundant enzyme globally, it is inefficient for carbon fixation because of its low turnover rate and limited ability to distinguish CO2 and O2, especially under high O2 conditions. To address these limitations, phytoplankton, including cyanobacteria and algae, have evolved CO2‐concentrating mechanisms (CCM) that involve compartmentalizing Rubisco within specific structures, such as carboxysomes in cyanobacteria or pyrenoids in algae. Engineering plant chloroplasts to establish similar structures for compartmentalizing Rubisco has attracted increasing interest for improving photosynthesis and carbon assimilation in crop plants. Here, we present a method to effectively induce the condensation of endogenous Rubisco within tobacco (Nicotiana tabacum) chloroplasts by genetically fusing superfolder green fluorescent protein (sfGFP) to the tobacco Rubisco large subunit (RbcL). By leveraging the intrinsic oligomerization feature of sfGFP, we successfully created pyrenoid‐like Rubisco condensates that display dynamic, liquid‐like properties within chloroplasts without affecting Rubisco assembly and catalytic function. The transgenic tobacco plants demonstrated comparable autotrophic growth rates and full life cycles in ambient air relative to the wild‐type plants. Our study offers a promising strategy for modulating endogenous Rubisco assembly and spatial organization in plant chloroplasts via phase separation, which provides the foundation for generating synthetic organelle‐like structures for carbon fixation, such as carboxysomes and pyrenoids, to optimize photosynthetic efficiency.
虽然Rubisco是全球最丰富的酶,但由于其周转率低,区分CO2和O2的能力有限,特别是在高氧条件下,其固定碳的效率较低。为了解决这些限制,浮游植物,包括蓝藻和藻类,已经进化出二氧化碳浓缩机制(CCM),包括在特定结构中分隔Rubisco,例如蓝藻中的羧基体或藻类中的类pyrenoids。工程植物叶绿体建立类似的结构来划分Rubisco已经引起了人们对改善作物光合作用和碳同化的兴趣。本研究提出了一种通过将超级文件夹绿色荧光蛋白(sfGFP)与烟草Rubisco大亚基(RbcL)基因融合,有效诱导内源性Rubisco在烟草叶绿体中缩合的方法。通过利用sfGFP固有的寡聚化特征,我们成功地创造了类pyrenoid - like Rubisco凝聚物,在叶绿体中显示出动态的,液体状的性质,而不影响Rubisco的组装和催化功能。与野生型烟草相比,转基因烟草在环境空气中表现出相当的自养生长速度和完整的生命周期。我们的研究为通过相分离调节植物叶绿体中内源性Rubisco组装和空间组织提供了一种有希望的策略,这为合成类似于碳固定的细胞器结构(如羧基体和类pyrenoids)提供了基础,以优化光合效率。
期刊介绍:
Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.