{"title":"水稻DOF转录因子GLW9/OsDOF25调控水稻籽粒形状和分蘖角度","authors":"Huan Shi, Pingbo Li, Peng Yun, Yun Zhu, Hao Zhou, Lu Wang, Bian Wu, Yipei Wang, Guangming Lou, Qin Huang, Guanjun Gao, Qinglu Zhang, Junxiao Chen, Jinbo Li, Jinghua Xiao, Aiqing You, Yuqing He","doi":"10.1111/pbi.70064","DOIUrl":null,"url":null,"abstract":"<p>Grain shape and tiller angle are two important agronomic traits influencing grain yield and quality in rice. Herein, we map-based cloned a grain shape gene <i>GLW9</i> (<i>Grain Length and Width on chromosome 9</i>), which encodes a DNA binding with one finger (DOF) family transcription factor OsDOF25. <i>GLW9</i> positively regulates grain length and negatively regulates grain width, consequently improving grain length-to-width ratio and appearance quality. GLW9 binds to the <i>EXPA6</i> promotor to upregulate its expression, thereby positively regulating cell expansion and grain shape. On the other hand, GLW9 directly upregulates the expression of <i>OsPIN1b</i> to reduce tiller angle. This study elucidates the mechanism by which <i>GLW9</i> coordinately regulates grain shape and tiller angle, providing theoretical reference and gene resources for the improvement of grain shape and tiller angle in rice.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"23 6","pages":"2367-2382"},"PeriodicalIF":10.1000,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70064","citationCount":"0","resultStr":"{\"title\":\"A DOF transcription factor GLW9/OsDOF25 regulates grain shape and tiller angle in rice\",\"authors\":\"Huan Shi, Pingbo Li, Peng Yun, Yun Zhu, Hao Zhou, Lu Wang, Bian Wu, Yipei Wang, Guangming Lou, Qin Huang, Guanjun Gao, Qinglu Zhang, Junxiao Chen, Jinbo Li, Jinghua Xiao, Aiqing You, Yuqing He\",\"doi\":\"10.1111/pbi.70064\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Grain shape and tiller angle are two important agronomic traits influencing grain yield and quality in rice. Herein, we map-based cloned a grain shape gene <i>GLW9</i> (<i>Grain Length and Width on chromosome 9</i>), which encodes a DNA binding with one finger (DOF) family transcription factor OsDOF25. <i>GLW9</i> positively regulates grain length and negatively regulates grain width, consequently improving grain length-to-width ratio and appearance quality. GLW9 binds to the <i>EXPA6</i> promotor to upregulate its expression, thereby positively regulating cell expansion and grain shape. On the other hand, GLW9 directly upregulates the expression of <i>OsPIN1b</i> to reduce tiller angle. This study elucidates the mechanism by which <i>GLW9</i> coordinately regulates grain shape and tiller angle, providing theoretical reference and gene resources for the improvement of grain shape and tiller angle in rice.</p>\",\"PeriodicalId\":221,\"journal\":{\"name\":\"Plant Biotechnology Journal\",\"volume\":\"23 6\",\"pages\":\"2367-2382\"},\"PeriodicalIF\":10.1000,\"publicationDate\":\"2025-03-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70064\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Biotechnology Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/pbi.70064\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology Journal","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/pbi.70064","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
粒形和分蘖角是影响水稻产量和品质的两个重要农艺性状。在此，我们基于图谱克隆了一个谷物形状基因GLW9 (grain Length and Width on chromosome 9)，该基因编码与一指（DOF）家族转录因子OsDOF25结合的DNA。GLW9正调控粒长，负调控粒宽，从而改善籽粒长宽比和外观品质。GLW9结合EXPA6启动子上调其表达，从而正向调节细胞扩增和颗粒形状。另一方面，GLW9直接上调OsPIN1b的表达，使分蘖角减小。本研究阐明了GLW9协调调控粒形和分蘖角的机制，为水稻粒形和分蘖角的改良提供理论参考和基因资源。
A DOF transcription factor GLW9/OsDOF25 regulates grain shape and tiller angle in rice
Grain shape and tiller angle are two important agronomic traits influencing grain yield and quality in rice. Herein, we map-based cloned a grain shape gene GLW9 (Grain Length and Width on chromosome 9), which encodes a DNA binding with one finger (DOF) family transcription factor OsDOF25. GLW9 positively regulates grain length and negatively regulates grain width, consequently improving grain length-to-width ratio and appearance quality. GLW9 binds to the EXPA6 promotor to upregulate its expression, thereby positively regulating cell expansion and grain shape. On the other hand, GLW9 directly upregulates the expression of OsPIN1b to reduce tiller angle. This study elucidates the mechanism by which GLW9 coordinately regulates grain shape and tiller angle, providing theoretical reference and gene resources for the improvement of grain shape and tiller angle in rice.
期刊介绍：
Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.
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