Plant Biotechnology Journal最新文献_第3页

A DOF transcription factor GLW9/OsDOF25 regulates grain shape and tiller angle in rice

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-22 DOI: 10.1111/pbi.70064

Huan Shi, Pingbo Li, Peng Yun, Yun Zhu, Hao Zhou, Lu Wang, Bian Wu, Yipei Wang, Guangming Lou, Qin Huang, Guanjun Gao, Qinglu Zhang, Junxiao Chen, Jinbo Li, Jinghua Xiao, Aiqing You, Yuqing He

引用次数: 0

Development of “Tea Rice” by engineering catechin biosynthesis in rice endosperm 通过在水稻胚乳中进行儿茶素生物合成工程培育 "茶稻

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-22 DOI: 10.1111/pbi.70060

Jinjin Zhu, Shen Zhou, Wenzhao Wang, Yuanyuan Lv, Chenkun Yang, Qiming Wang, Ran Zhang, Shuangqian Shen, Jie Luo

引用次数: 0

Teach plants to fish based on CRISPR‐Cas system self‐evolution 基于 CRISPR-Cas 系统的自我进化，让植物学会捕鱼

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-22 DOI: 10.1111/pbi.70066

Xuhui Ma, Liqing Miao, Xiaoqing Liu

引用次数: 0

Mitochondrial gene editing and allotopic expression unveil the role of orf125 in the induction of male fertility in some Solanum spp. hybrids and in the evolution of the common potato

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-22 DOI: 10.1111/pbi.70012

Rachele Tamburino, Nunzio D'Agostino, Gaetano Aufiero, Alessandro Nicolia, Angelo Facchiano, Deborah Giordano, Lorenza Sannino, Rosa Paparo, Shin-Ichi Arimura, Nunzia Scotti, Teodoro Cardi

{"title":"Mitochondrial gene editing and allotopic expression unveil the role of orf125 in the induction of male fertility in some Solanum spp. hybrids and in the evolution of the common potato","authors":"Rachele Tamburino, Nunzio D'Agostino, Gaetano Aufiero, Alessandro Nicolia, Angelo Facchiano, Deborah Giordano, Lorenza Sannino, Rosa Paparo, Shin-Ichi Arimura, Nunzia Scotti, Teodoro Cardi","doi":"10.1111/pbi.70012","DOIUrl":"https://doi.org/10.1111/pbi.70012","url":null,"abstract":"Genic-cytoplasmic male sterility (CMS) due to interactions between nuclear and cytoplasmic genomes is a well-known phenomenon in some Solanum spp. hybrids, but genes involved are not known. In this study, the chondriomes of two isonuclear male-fertile and sterile somatic hybrids (SH9A and SH9B, respectively) between the common potato (S. tuberosum Group Tuberosum, tbr) and the wild species S. commersonii were sequenced and compared to those of parental species to identify mitochondrial genes involved in the expression of male sterility. A putative novel gene (orf125) was found only in tbr and in male-sterile hybrids. Physical or functional deletion of orf125 by mtDNA editing in SH9B and its allotopic expression in SH9A clearly demonstrated that orf125 affects male fertility. Besides knockout mutants induced by mitoTALEN and DddA-derived cytosine base editing, specific orf125 missense mutations generated by the latter approach also induced reversion to male fertility in edited SH9B plants, prompting further studies on ORF125 structure–function relationship. The organization of the mitochondrial genome region implicated in CMS was found to be conserved across all common potato accessions, while an identical copy of tbr orf125 was detected in accessions belonging to the S. berthaultii species complex (ber). Such findings corroborate the hypothesis that ber accessions with T/β cytoplasm outcrossed as female with Andean potato, giving rise to the differentiation of the Chilean potato, and highlight the origin of mitochondrial factors contributing to genic-cytoplasmic male sterility in some tuber-bearing Solanum hybrids. Our results contribute to the development of innovative breeding approaches in potato.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"214 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic manipulation of a COBRA gene, PtrCOB11, substantially alters wood properties in poplar 对 COBRA 基因 PtrCOB11 的遗传操作可大幅改变杨树的木材特性

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-22 DOI: 10.1111/pbi.70068

Wenjing Xu, Hao Cheng, Siran Zhu, Chong Wang, Jiyao Cheng, Mengjie Guo, Nabil Ibrahim Elsheery, Xingguo Lan, Yuxiang Cheng

引用次数: 0

Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-20 DOI: 10.1111/pbi.70057

Sunok Moon, Yang-Seok Lee, Jose Gutierrez-Marcos, Ki-Hong Jung

{"title":"Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture","authors":"Sunok Moon, Yang-Seok Lee, Jose Gutierrez-Marcos, Ki-Hong Jung","doi":"10.1111/pbi.70057","DOIUrl":"https://doi.org/10.1111/pbi.70057","url":null,"abstract":"Rice serves as a staple food for approximately half of the world's population, and enhanced yields from hybrid rice play a crucial role in ensuring food security and augmenting incomes. However, the annual purchase and high cost of hybrid seeds hinder widespread hybrid rice adoption. In this review, we discuss hybrid seed production strategies based on molecular mechanisms along with biotechnological techniques employed for production and future prospects. Male-sterile lines are pivotal in hybrid seed production, with ongoing developments markedly advancing this process. Initially, cytoplasmic male-sterile lines facilitated three-line hybrid seed production. Subsequent innovations, including environmentally responsive gene-based and biotechnology-driven male-sterile lines, enabled two-line hybrid rice production. Ongoing research is focusing on implementing a one-line hybrid seed production method using apomixis, driving innovation in hybrid seed production. Overall, advancements in male-sterile lines and synthetic apomixis present promising avenues for improving the efficiency and sustainability of hybrid rice production. These developments highlight the critical need for continued research and concerted efforts to address global food security challenges.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"26 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rubber biosynthesis drives the biogenesis and development of rubber particles, the rubber-producing organelles

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-20 DOI: 10.1111/pbi.70052

Tiancheng Xu, Yongmei Li, Xing Liu, Xue Yang, Zhejun Huang, Jianfeng Xing, Cuili Liang, Junyi Li, Yingchao Tan, Shengmin Zhang, Jiyan Qi, De Ye, Zhonghua Li, Jie Cao, Chaorong Tang, Kaiye Liu

{"title":"Rubber biosynthesis drives the biogenesis and development of rubber particles, the rubber-producing organelles","authors":"Tiancheng Xu, Yongmei Li, Xing Liu, Xue Yang, Zhejun Huang, Jianfeng Xing, Cuili Liang, Junyi Li, Yingchao Tan, Shengmin Zhang, Jiyan Qi, De Ye, Zhonghua Li, Jie Cao, Chaorong Tang, Kaiye Liu","doi":"10.1111/pbi.70052","DOIUrl":"https://doi.org/10.1111/pbi.70052","url":null,"abstract":"Rubber particles (RPs) are specialized organelles for the biosynthesis and storage of natural rubber in rubber-producing plants. However, the mechanisms underlying the biogenesis and development of RPs remain unclear. In this study, two latex-specific cis-prenyltransferases (CPTs), TkCPT1 and TkCPT2, were identified in Taraxacum kok-saghyz, with almost identical orthologues retained across other Taraxacum species. For the first time, Tkcpt1 single and Tkcpt1/2 double mutants were successfully generated using the CRISPR/Cas9 system. Rubber biosynthesis was significantly depressed in Tkcpt1 mutants and completely blocked in Tkcpt1/2 mutants. The absence of RPs in the Tkcpt1/2 was confirmed using oil red O and Nile red staining, high-speed centrifugal stratification, cryo-SEM and TEM on fresh latex or laticifer cells. Transcriptomic and proteomic analyses revealed that, in the latex of Tkcpt1/2, rubber biosynthesis was blocked at the protein level, while metabolomic profiling indicated an enrichment of lipids and terpenoids. Furthermore, knockout of TkCPTL1, a latex-specific CPT-like gene that encodes a rubber transferase activator, resulted in outright disruption of rubber biosynthesis and RP ontogeny, a phenotype similar to that of Tkcpt1/2 mutants. These findings indicate that rubber biosynthesis is a driving force for the biogenesis and development of RPs, providing new insights into rubber production mechanisms.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"16 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Telomere-to-telomere genome assembly reveals insights into the adaptive evolution of herbivore-defense mediated by volatile terpenoids in Oenanthe javanica

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-20 DOI: 10.1111/pbi.70062

Kai Feng, Jia-Lu Liu, Nan Sun, Zi-Qi Zhou, Zhi-Yuan Yang, Hui Lv, Cheng Yao, Jin-Ping Zou, Shu-Ping Zhao, Peng Wu, Liang-Jun Li

{"title":"Telomere-to-telomere genome assembly reveals insights into the adaptive evolution of herbivore-defense mediated by volatile terpenoids in Oenanthe javanica","authors":"Kai Feng, Jia-Lu Liu, Nan Sun, Zi-Qi Zhou, Zhi-Yuan Yang, Hui Lv, Cheng Yao, Jin-Ping Zou, Shu-Ping Zhao, Peng Wu, Liang-Jun Li","doi":"10.1111/pbi.70062","DOIUrl":"https://doi.org/10.1111/pbi.70062","url":null,"abstract":"Releasing large quantities of volatiles is a defense strategy used by plants to resist herbivore attack. Oenanthe javanica, a perennial herb of the Apiaceae family, has a distinctive aroma due to volatile terpenoid accumulation. At present, the complete genome and genetic characteristics of volatile terpenoids in O. javanica remain largely unclear. Here, the telomere-to-telomere genome of O. javanica, with a size of 1012.13 Mb and a contig N50 of 49.55 Mb, was established by combining multiple sequencing technologies. Comparative genome analysis revealed that O. javanica experienced a recent species-specific whole-genome duplication event during the evolutionary process. Numerous gene family expansions were significantly enriched in the terpenoid biosynthesis process, monoterpenoid, and diterpenoid biosynthesis pathways, which resulted in abundant volatile substance accumulation in O. javanica. The volatile terpenoids of O. javanica showed repellent effects on herbivores. Terpenoid biosynthesis was activated by wounding signals under exogenous stimuli. The TPS gene family was significantly expanded in O. javanica compared to those in other species, and the members (OjTPS1, OjTPS3, OjTPS4, OjTPS5, OjTPS7, OjTPS16, OjTPS18, OjTPS30 and OjTPS58) responsible for different terpenoid biosynthesis were functionally characterized. These results reveal the genome evolution and molecular characteristics of volatile terpenoids in the process of plant–herbivore interactions. This study also provides genomic resources for genetic and molecular biology research on O. javanica and other plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"3 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing glycosylase‐based T‐to‐G and C‐to‐K base editors in rice

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-20 DOI: 10.1111/pbi.70063

Yongjie Kuang, Xuemei Wu, Meijie Liu, Fang Yan, Dongfang Ma, Xueping Zhou, Huanbin Zhou, Bin Ren

引用次数: 0

GmAKT1-mediated K+ absorption positively modulates soybean salt tolerance by GmCBL9-GmCIPK6 complex

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-03-20 DOI: 10.1111/pbi.70042

Chen Feng, Muhammad Azhar Hussain, Yan Zhao, Yuning Wang, Yuyan Song, Yaxin Li, Hongtao Gao, Yan Jing, Keheng Xu, Wenping Zhang, Yonggang Zhou, Haiyan Li

{"title":"GmAKT1-mediated K+ absorption positively modulates soybean salt tolerance by GmCBL9-GmCIPK6 complex","authors":"Chen Feng, Muhammad Azhar Hussain, Yan Zhao, Yuning Wang, Yuyan Song, Yaxin Li, Hongtao Gao, Yan Jing, Keheng Xu, Wenping Zhang, Yonggang Zhou, Haiyan Li","doi":"10.1111/pbi.70042","DOIUrl":"https://doi.org/10.1111/pbi.70042","url":null,"abstract":"Soybean is one of the most important crops in the world. However, salt stress poses a major challenge to soybean growth and productivity. Therefore, unravelling the complex mechanisms governing salt tolerance in soybean is imperative for molecular breeding of salt-tolerant varieties to improve yield. Maintaining intracellular Na+/K+ homeostasis is one of the key factors for plant salt tolerance. Although some salt tolerance mechanisms involving Na+ exclusion have been well identified in plants, few studies have been conducted on how K+ influx controls soybean salt tolerance. Here, we characterized the function of soybean K+ channel gene GmAKT1 and identified GmCBL9-GmCIPK6 complex, which modulated GmAKT1-mediated K+ uptake under salt stress. Functional studies found that soybean lines GmAKT1 overexpressing increased K+ content and promoted salt tolerance, while CRISPR/Cas9-mediated disruption of GmAKT1 soybean lines decreased the K+ content and showed salt sensitivity. Furthermore, we identified that GmCIPK6 interacted with GmAKT1 and GmCBL9 interacted with GmCIPK6. In addition, Mn2+-Phos-tag assays proved that GmCIPK6 could phosphorylate GmAKT1. This collaborative activation of the GmCBL9-GmCIPK6-GmAKT1 module promoted K+ influx and enhanced soybean salt tolerance. Our findings reveal a new molecular mechanism in soybeans under salt stress and provide insights for cultivating new salt-tolerant soybean varieties by molecular breeding.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"15 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0