Plant Biotechnology Journal最新文献

{"title":"Identification of Critical Amino Acid Residues Required for the Polar Localization of a Rice Manganese Transporter","authors":"Noriyuki Konishi,&nbsp;Jian Feng Ma","doi":"10.1111/pbi.70708","DOIUrl":"10.1111/pbi.70708","url":null,"abstract":"<p>Rice has developed an efficient system for manganese (Mn) uptake, mediated by two distinct transporters, OsNramp5 and OsMTP9. These transporters exhibit polar localization at the root exodermis and endodermis; however, the mechanisms underlying their polar localization and their role in Mn uptake remain unclear. Here, we identified key amino acid residues critical for the polar localization of OsNramp5 at the distal side. Through analysis of chimeric proteins between OsNramp5 and its non-polar homologues, we found that the C-terminal cytosolic region of OsNramp5 is essential for its polar localization. Site-directed mutagenesis further revealed that aspartate 500 and four valine residues at positions 494, 495, 498 and 506 are crucial for polarity. Substitution of these valine residues with isoleucine, leucine, phenylalanine, or threonine partially or fully maintained polar localization, whereas substitution with alanine, serine, or asparagine resulted in loss of polarity. These findings suggest that β-branching and high hydrophobicity of amino acid side chains are likely required for OsNramp5 polarity. Furthermore, we found that adaptor protein 2-dependent clathrin-mediated endocytosis is not involved in the polar localization of OsNramp5. Finally, we provided experimental evidence showing the significant role of OsNramp5 polarity in efficient Mn uptake in rice; plants expressing non-polarly localized OsNramp5 exhibited reduced Mn uptake compared to those with polarly localized OsNramp5. In addition, we found that cadmium accumulation in shoots could be reduced by manipulating OsNramp5 polarity in combination with its overexpression, without a growth penalty.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 10","pages":"5340-5351"},"PeriodicalIF":12.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148313883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Robust Framework for Maize Elite Line Genome Editing Through Enhanced HI-Edit via LbCas12a Activity Optimization","authors":"Dawei Liang,&nbsp;Huanhuan Guo,&nbsp;Juan Wei,&nbsp;Fugui Zhu,&nbsp;Yuguo Zhang,&nbsp;Julie Green,&nbsp;Yun Ji,&nbsp;Huaibing Jin,&nbsp;Xiujuan Zhang,&nbsp;Huaping Gui,&nbsp;Hongmei Dan,&nbsp;Yubo Liu,&nbsp;Yu Zhang,&nbsp;Han Wang,&nbsp;Yutong Jiang,&nbsp;Lizhao Geng,&nbsp;Jian Lv,&nbsp;Wen Cai,&nbsp;Weibin Song,&nbsp;Timothy Kelliher,&nbsp;Xi Chen,&nbsp;Rachel Egger","doi":"10.1111/pbi.70715","DOIUrl":"10.1111/pbi.70715","url":null,"abstract":"<p>Haploid induction coupled with genome editing (HI-Edit) enables direct modification of commercial crop varieties, bypassing the need for trait introgression or direct transformation of elite lines with CRISPR machinery. However, its widespread application has been constrained by low haploid editing rates (HER), the proportion of haploids carrying edits within the short window between double fertilization and uniparental chromosome elimination. Here, we report substantial improvements in maize HI-Edit efficiency through three complementary strategies: (1) driving an optimized LbCas12a variant (LbCas12aV) using promoters that are highly active in sperm cells and early zygotes; (2) applying a post-pollination heat treatment; and (3) fusing LbCas12aV with the UBA2 domain (ubiquitin-associated domain-2 of <i>Arabidopsis thaliana</i> RAD23) to enhance protein stability during haploid induction. Post-pollination heat treatment alone increased HER to 19.1% (up to 12-fold improvement depending on the target site), providing a simple and effective method to boost the yield of edited doubled haploid (DH) plants. UBA2 fusion improved HER by 6-fold at the <i>Waxy1</i> (<i>Wx1</i>) locus and 4.5-fold at the <i>Glossy2</i> (<i>Gl2</i>) locus under normal conditions. Strikingly, combining UBA2 fusion with heat treatment raised the average HER to 25% across multiple events targeting <i>Wx1</i>, with the highest HER reaching 33%. Collectively, these findings demonstrate that increasing CRISPR-Cas protein abundance and modulating environmental conditions can overcome key bottlenecks in HI-Edit. We establish a robust, scalable framework that is readily transferable to other crops for elite-line genome editing.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 10","pages":"5392-5404"},"PeriodicalIF":12.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70715","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148379936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CaM1-CBP60b-MYB77 Transcriptional Cascade Regulates K+ Homeostasis and Salt Tolerance in Barley","authors":"Yunfeng Xu,&nbsp;Haoran Sun,&nbsp;Lijun Liu,&nbsp;Qiyu Liang,&nbsp;Ling Shen,&nbsp;Ailing Han,&nbsp;Liangbo Fu,&nbsp;Fengyue Wang,&nbsp;Shanggeng Xie,&nbsp;Jiangyan Xiong,&nbsp;Yishan Tu,&nbsp;Zhengyuan Xu,&nbsp;Kangfeng Cai,&nbsp;Lingzhen Ye,&nbsp;Shengguan Cai,&nbsp;Zhong-Hua Chen,&nbsp;Dezhi Wu,&nbsp;Guoping Zhang,&nbsp;Qiufang Shen","doi":"10.1111/pbi.70693","DOIUrl":"10.1111/pbi.70693","url":null,"abstract":"<p>Soil salinity is a major abiotic stress that threatens global crop productivity. Calcium (Ca²⁺) is a pivotal second messenger in plant stress signalling, but how Ca²⁺ sensors such as calmodulin (CaM) transcriptionally control ion homeostasis and salt tolerance remains largely understood in crops. Here, we identified a novel calmodulin-binding protein HvCBP60b, which is a negative regulator of salt resilience in salt-tolerant barley. We showed that HvCBP60b interacts with Ca²⁺ sensor HvCaM1 via its C-terminal domain in a Ca²⁺-dependent manner. Loss of either <i>HvCBP60b</i> or <i>HvCaM1</i> promoted the uptake of K⁺, increased the K⁺/Na⁺ ratio, and markedly enhanced both growth performance and grain yield under salinity stress. Furthermore, HvCBP60b binds to the promoter of R2R3-type MYB transcription factor <i>HvMYB77</i> through its N-terminal domain. HvMYB77 then transcriptionally reduces the expression of <i>HvHAK5,</i> a high-affinity K⁺ transporter essential for maintaining K⁺ homeostasis under salinity. Mutations in <i>HvMYB77</i> or <i>HvHAK5</i> compromise salt tolerance, whereas the salt-sensitive phenotype of <i>hvhak5</i> is rescued in <i>hvcbp60b-hvhak5</i> double mutants. Therefore, we uncover a novel Ca²⁺-responsive regulatory module CaM1-CBP60b-MYB77-HAK5 essential for K⁺ homeostasis and salt tolerance in barley, highlighting new targets of Ca²⁺ signalling for improving salt resilience in cereal crops.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 10","pages":"5248-5265"},"PeriodicalIF":12.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148222364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics","authors":"Elisabetta Mazzucotelli,&nbsp;Cristian Forestan,&nbsp;Gina Zastrow-Hayes,&nbsp;David Swarbreck,&nbsp;Yael Lev-Mirom,&nbsp;Emile Cavalet-Giorsa,&nbsp;Matteo Bozzoli,&nbsp;Anna Maria Mastrangelo,&nbsp;Daniela Marone,&nbsp;Vincent Ranwez,&nbsp;Johanna Girodolle,&nbsp;Victor Llaca,&nbsp;Kevin Fengler,&nbsp;Charlotte Harris,&nbsp;Helena Toegelová,&nbsp;Pavla Navrátilová,&nbsp;Primetta Faccioli,&nbsp;Francesca Desiderio,&nbsp;Ana Paola Valladares,&nbsp;Gemy Kaithakottil,&nbsp;Rachel L. Rusholme-Pilcher,&nbsp;Manuel Spannagl,&nbsp;Heidrun Gundlach,&nbsp;Klaus F. X. Mayer,&nbsp;Victoria C. Blake,&nbsp;Justin D. Faris,&nbsp;Steven S. Xu,&nbsp;Taner Z. Sen,&nbsp;Eric Yao,&nbsp;Julio Isidro y Sánchez,&nbsp;Chunyi Liu,&nbsp;Muhammad A. Farooq,&nbsp;Sandra Stefanelli,&nbsp;Chiara Cappucci,&nbsp;Leah Oren,&nbsp;Tamar Eilam,&nbsp;Mario Giorgioni,&nbsp;Alessandra Stella,&nbsp;Barbara Lazzari,&nbsp;Massimiliano Lauria,&nbsp;Aldo Ceriotti,&nbsp;Raul Pirona,&nbsp;Elahe Tavakol,&nbsp;Valentyna Klymiuk,&nbsp;Jennifer Ens,&nbsp;Harmeet Singh Chawla,&nbsp;Sean Walkowiak,&nbsp;Nicola Pecchioni,&nbsp;Filippo M. Bassi,&nbsp;Miguel Sanchez-Garcia,&nbsp;Gabriella Sonnante,&nbsp;Pasquale L. Curci,&nbsp;Giovanni Giuliano,&nbsp;Giuseppe Aprea,&nbsp;Asher Pasha,&nbsp;Agata Gadaleta,&nbsp;Ilaria Marcotuli,&nbsp;Stefania Masci,&nbsp;Francesco Sestili,&nbsp;Samuela Palombieri,&nbsp;Davide Scaglione,&nbsp;Michele Morgante,&nbsp;Hana Šimková,&nbsp;Nicholas J. Provart,&nbsp;Simon G. Krattinger,&nbsp;Curtis J. Pozniak,&nbsp;Nathalie Chantret,&nbsp;Anthony Hall,&nbsp;Assaf Distelfeld,&nbsp;Roberto Tuberosa,&nbsp;Marco Maccaferri,&nbsp;Luigi Cattivelli","doi":"10.1111/pbi.70673","DOIUrl":"10.1111/pbi.70673","url":null,"abstract":"<p>Advancements in plant genome sequencing and assembly have enabled the production of increasingly accurate and contiguous genome sequences. Here, we present the chromosome-level assembly of the durum wheat (<i>Triticum turgidum</i> L. ssp. <i>durum</i>, cv. Svevo) reference genome produced using accurate long-reads, optical mapping and Hi-C. The new assembly (Svevo Rel.2.0) comprises 263 hybrid scaffolds with an N50 value of 112.3 Mb, arranged into 14 contiguous pseudomolecules spanning 10.4 Gb. The Svevo Rel.2.0 genome assembly was annotated using extensive short- and long-read RNA sequencing data obtained from 60 tissue/treatment combinations. The resulting annotation comprises 68 154 high-confidence protein-coding genes, which have been integrated into a comprehensive transcriptome atlas accessible through an eFP browser. Annotation was manually curated for storage protein gene families and for Leucine-Rich Repeat-Containing Receptor genes yielding 3763 LRR-CR loci. The genome assembly's accuracy and completeness were demonstrated by the correct reconstruction of the physical map of <i>Tg1-B</i> (<i>Tenacious glumes 1</i>), a locus controlling the free threshing trait located on chromosome 2B that was not assembled in the previous genome release (Svevo Rel.1.0). A wealth of 6621 QTLs/MTAs from the literature were mapped onto Svevo Rel.2.0 to identify QTL hotspots and trait-specific candidate genes. The ancestry of the durum genome to representative wild emmer populations from North-Eastern and Southern-Levant Fertile Crescent assessed by tracing haplotype transmission patterns revealed a clear mosaic pattern. This new durum reference genome, enhanced with advanced annotation and an expression atlas linked to QTLome data, is the most comprehensive tool available for durum wheat genomics.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 10","pages":"5460-5479"},"PeriodicalIF":12.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70673","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Modulation of Eukaryotic Release Factor 1 (OseRF1) by an RNA Aptamer Enhances Drought Adaptation in Rice","authors":"Shuangfeng Dai,&nbsp;Mingming Chen,&nbsp;Haomin Chen,&nbsp;Yuantao Ye,&nbsp;Hong Huang,&nbsp;Renrong Zhang,&nbsp;Zhenyu Guo,&nbsp;Yongxiang Huang","doi":"10.1111/pbi.70720","DOIUrl":"10.1111/pbi.70720","url":null,"abstract":"<p>Drought stress severely restricts rice productivity, necessitating strategies to enhance stress resilience. Here, we identify translation termination as a tunable regulatory node for stress adaptation. Using 25 rounds of SELEX, we isolated RTAR, an RNA aptamer that binds the rice eukaryotic release factor 1 (OseRF1) with nanomolar affinity. Structural and functional analyses revealed that RTAR targets the OseRF1 N-domain through a conserved pocket centered on Arg46, inhibiting stop-codon recognition and promoting translational readthrough in rice cell extracts. Under drought stress, RTAR co-localized with OseRF1 in rice protoplasts and accelerated its depletion, thereby reprogramming termination fidelity. Ribosome profiling (Ribo-seq) identified candidate genes, including OsDR8, with increased ribosome occupancy downstream of annotated stop codons, indicating a transcriptome-wide effect on translation termination. RTAR expression significantly enhanced drought tolerance in rice seedlings, as evidenced by increased survival, chlorophyll retention and reduced membrane injury. These findings were further validated in stable transgenic lines subjected to soil-based drought stress, where RTAR-expressing plants exhibited enhanced whole-plant drought resilience. This phenotype was accompanied by upregulation of drought-responsive genes and was abolished in OseRF1-R46A mutants, demonstrating the functional importance of the Arg46-centered binding pocket. Furthermore, GFP-tagging assays revealed higher-molecular-weight OsDR8 isoforms consistent with C-terminal extension, providing direct evidence of translational readthrough in vivo. Together, these results establish RTAR as a precise and reversible modulator of translation termination and identify OseRF1 as a promising target for engineering drought adaptation in crops.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 10","pages":"5440-5459"},"PeriodicalIF":12.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70720","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148400713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Variation in GmSL20 Improves Seed Size and Quality in Soybean","authors":"Yuechuan Luo,&nbsp;Zongbiao Duan,&nbsp;Juxu Li,&nbsp;Zhou Zhu,&nbsp;Tong Wu,&nbsp;Dan Li,&nbsp;Yangping Liu,&nbsp;Liangwei Xu,&nbsp;Hao Wen,&nbsp;Yanting Shen,&nbsp;Shulin Liu,&nbsp;Qingtian Li,&nbsp;Zhixi Tian","doi":"10.1111/pbi.70701","DOIUrl":"10.1111/pbi.70701","url":null,"abstract":"<p>Seed size plays an important role in determining crop yield, yet the genetic mechanisms underlying the regulation of this agronomic trait remain incompletely understood in soybean. Here, we perform a genome-wide association study mapping with seed length variations and identify a major quantitative trait locus (QTL) on chromosome 20, named <i>SL20</i> (<i>Seed Length 20</i>). <i>GmSL20</i> encodes a TGA-type bZIP transcription factor that controls soybean seed size and weight in natural populations. Knockout of <i>GmSL20</i> suppresses seed enlargement and lipid biosynthesis in soybean, whereas increased expression of <i>GmSL20</i> improves seed size and oil content. Further functional investigations reveal that GmSL20 directly activates <i>GmST05</i> and <i>GmZF351</i>, thus promoting carbon partitioning to seeds. A super allele that arose from domestication confers elevated capacity for DNA binding and transcriptional activation to GmSL20. It correlates with larger seed size in haplotype analysis and enhances seed size and weight in elite soybean variety; however, it has not been widely fixed and spread through modern breeding. The role of <i>GmSL20</i> to promote yield and quality underscores its potential as a valuable resource for soybean breeding programs seeking to improve both traits simultaneously.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 10","pages":"5300-5316"},"PeriodicalIF":12.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148269671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-Mediated Editing of Four Ca2+-Binding Receptor-Like Cytoplasmic Kinases Improves Soybean Seed Size and Yield at Dense Planting","authors":"Wanhong Li,&nbsp;Jun Jin,&nbsp;Tianyue Gu,&nbsp;Xiaoxi Cai,&nbsp;Bowei Jia,&nbsp;Yan Wang,&nbsp;Dezhi Han,&nbsp;Xiaofei Yan,&nbsp;Xiping Hu,&nbsp;Xiaoli Sun,&nbsp;Sijia Lu,&nbsp;Mingzhe Sun","doi":"10.1111/pbi.70713","DOIUrl":"10.1111/pbi.70713","url":null,"abstract":"&lt;p&gt;Soybean serves as a crucial provider of both edible oil and vegetable protein for humans. Nevertheless, the grain yield of soybean at the population level lags significantly behind that of other staple crops (Tian et al. &lt;span&gt;2025&lt;/span&gt;). High-density planting is critical for improving soybean yield (Li, Sun, et al. &lt;span&gt;2023&lt;/span&gt;), however, few genes have been functionally characterised to confer soybean yield under dense planting.&lt;/p&gt;&lt;p&gt;Receptor like protein kinase functions in controlling diverse biological processes in plants, including reproduction (Cui et al. &lt;span&gt;2022&lt;/span&gt;), stress response and immunity (Hailemariam et al. &lt;span&gt;2024&lt;/span&gt;). Previously, four Ca&lt;sup&gt;2+&lt;/sup&gt;-binding receptor like cytoplasmic kinases from soybean GmCRCK1a-1d were reported to be involved in salt-alkaline tolerance (Sun et al. &lt;span&gt;2021&lt;/span&gt;). Interestingly, they show much lower expression levels in developing seeds compared with other vegetative organs (Figure S1), indicating possible roles in seed development. The smaller seeds of &lt;i&gt;GmCRCK1d&lt;/i&gt; overexpression (GmCRCK1d-OX) &lt;i&gt;Arabidopsis&lt;/i&gt; lines (Figure S2) supported the repression of &lt;i&gt;GmCRCK1d&lt;/i&gt; on seed development. To confirm it, we generated GmCRCK1d-OX soybean lines in the Dongnong50 (DN50) background (Figure S3). GmCRCK1d-OX lines showed a significant decrease in 100-seed weight, seed length, width and thickness (Figure S4). Given the high sequence similarity in both CDS and amino acid sequence among &lt;i&gt;GmCRCK1a-1d&lt;/i&gt; (Figure S5), we designed four gRNAs to generate &lt;i&gt;GmCRCK1a-1d&lt;/i&gt; mutants simultaneously via CRISPR/Cas9-mediated editing (Figure 1a, Figure S6a,b). Finally, eight &lt;i&gt;Cas9&lt;/i&gt;-free homozygous lines, including four tetra mutants (represented as &lt;i&gt;abcd&lt;/i&gt;) and four triple mutants (&lt;i&gt;abd&lt;/i&gt; or &lt;i&gt;abc&lt;/i&gt;), were identified in T&lt;sub&gt;2&lt;/sub&gt; generation (Figure 1a, Figures S6c, S7, S8). Interestingly, independent lines have identical deletion sizes, possibly due to the high homology among &lt;i&gt;GmCRCK1a-1d&lt;/i&gt;. These mutant lines had larger seeds compared with wild-type DN50, as evidenced by a significant increase in seed length, width, thickness and 100-seed weight (Figure 1b).&lt;/p&gt;&lt;p&gt;To elucidate the regulation of &lt;i&gt;GmCRCK1a-1d&lt;/i&gt; on soybean seed development, we performed a time-course analysis. The results showed that seeds of &lt;i&gt;GmCRCK1a-1d&lt;/i&gt; mutant lines displayed slower development and smaller size at 25 DAF (days after flowering) and later (Figure S9). Then we performed phosphoproteomic analysis with the &lt;i&gt;abcd&lt;/i&gt; #4 mutant line, to elucidate the &lt;i&gt;GmCRCK1a-1d-&lt;/i&gt;mediated molecular mechanism in controlling seed development. We detected 7665 phosphopeptides corresponding to 12 453 phosphorylation sites on 4148 proteins, with 1163 differentially phosphorylated proteins that were significantly enriched in 17 KEGG pathways related to nitrogen, carbon and fatty acid metabolism (Figure S10). Notably, eight phosphoproteins are related to the ER-associated degradat","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 10","pages":"5369-5371"},"PeriodicalIF":12.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70713","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148379737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AhMADS6-Mediated Repression of AhHD-Zip Contributes to Growth Habit Regulation in Cultivated Peanut (Arachis hypogaea L.).","authors":"Yuanjin Fang, Lei Shi, Boyang Zhang, Han Liu, Xiaobo Wang, Pengyu Qu, Huanhuan Zhao, Lulu Xue, Xiaona Li, Feiyan Qi, Ziqi Sun, Zheng Zheng, Xiaodong Dai, Wenzhao Dong, Bingyan Huang, Xinyou Zhang","doi":"10.1111/pbi.70760","DOIUrl":"10.1111/pbi.70760","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13595818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Converging GWAS and Genome Editing Decrypts MsCBF8-MsGolS2 Regulatory Module for Drought Tolerance Without Yield Penalty in Medicago sativa.","authors":"Yangyang Zhang, Xueqian Jiang, Tian Zhang, Huajuan Leng, Jing Cui, Xu Jiang, Lili Zhang, Zhen Wang, Tiejun Zhang, Qingchuan Yang, Xue Wang, Junmei Kang","doi":"10.1111/pbi.70756","DOIUrl":"10.1111/pbi.70756","url":null,"abstract":"<p><p>Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome-wide association study (GWAS) was conducted in alfalfa, which identified a CBF/DREB1 family transcription factor designated MsCBF8. Utilizing an optimized CRISPR-Cas9 system, mutants were successfully generated in the complex autotetraploid background. Combined with overexpression and Arabidopsis complementation assays, this confirmed that MsCBF8 functions as a positive regulator of drought tolerance, with its overexpression enhancing drought tolerance without compromising plant growth. Physiological analyses revealed that MsCBF8 improves drought tolerance by coordinating antioxidant defence (elevated SOD and CAT activities, decreased H₂O₂ and MDA contents), dynamically regulating stomatal movement (increased water-use efficiency), and maintaining photosynthetic performance. At the molecular level, MsCBF8 directly and specifically binds to the GCCGAC cis-element in the MsGolS2 promoter and activates its expression. Silencing MsGolS2 via virus-induced gene silencing (VIGS) reduced drought tolerance and aggravated oxidative damage, providing the first functional evidence for the essential role of this gene in the drought response of alfalfa. This study systematically elucidates a novel MsCBF8-MsGolS2 regulatory module, offering crucial insights into the drought adaptation mechanisms in alfalfa and providing valuable genetic resources and breeding targets for developing high-yield, water-efficient and drought-tolerant alfalfa cultivars.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling Mechanisms of Acquired Drought Tolerance by Priming in Tomato Using Integrative Multi-Omics Phenotypic Analysis.","authors":"Rong Zhou, Yankai Li, Ge Wang, Hong Chen, Xiaqing Yu, Yinlei Wang, Xuedong Yang, Zhen Lyu, Trupti Joshi, Xiaoming Song, Carl-Otto Ottosen, Ian Charles Dodd, Ron Mittler, Fangling Jiang, Zhen Wu","doi":"10.1111/pbi.70757","DOIUrl":"10.1111/pbi.70757","url":null,"abstract":"<p><p>The escalating frequency and intensity of recent droughts threaten global crop production and food security. Although drought priming is crucial for plant drought resilience, the mechanisms imparting drought stress memory remain poorly understood, especially in crops such as tomato. This study elucidates the mechanisms of drought stress memory in tomato by integrating phenotypic screening with transcriptomic and proteomic analyses. Initial screening of 30 genotypes identified varieties with contrasting basal drought tolerance as indicated by gas exchange and drought damage index. Subsequent priming experiments revealed that acquired drought tolerance is independent of basal resistance, with both sensitive and tolerant genotypes exhibiting enhanced adaptation. Priming improved photosynthetic capacity and water use efficiency via optimised stomatal regulation. Moreover, elevated reactive oxygen species (ROS), particularly H₂O₂, was a key signalling molecule that initiated the drought memory establishment. This acquired drought tolerance was stress-specific, enhancing tolerance of tomato plants to subsequent drought but not heat stress. Integrative multi-omics analysis identified 518 memory-associated transcripts and pinpointed key candidate genes, including HSP90 (heat shock protein 90), WRKY26 and WRKY31 (WRKY transcription factor 26/31), which were linked to central pathways such as MAPK (mitogen-activated protein kinase) signalling and phenylpropanoid biosynthesis. The VIGS (Virus-Induced Gene Silencing) assay further demonstrated that WRKY26 functions as a negative regulator of acquired drought tolerance. Our work established a mechanistic framework for drought stress memory, highlighting ROS signalling and memory-specific gene networks. These insights, along with the generated datasets, provide valuable resources for the strategic breeding of climate-resilient tomato varieties.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}