Plant Biotechnology Journal最新文献

{"title":"Chromosome‐scale haplotype‐resolved genome assembly of the autotetraploid alfalfa cultivar Bolivia","authors":"Hongkui Zhang, Lan Zhou, Hong Zhao, Jiayan Liang, Yongle Liu, Chen Wang, Sijie Sun, Lizhen Song, Yu'e Zhang, Youfa Cheng, Yongbiao Xue","doi":"10.1111/pbi.70259","DOIUrl":"https://doi.org/10.1111/pbi.70259","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"32 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"APPi: A Multiscale Qualitative–Quantitative Insecticide‐Likeness Evaluation Platform and Application","authors":"Jia‐lin Cui, Qi He, Bin‐yan Jin, Xin‐peng Sun, Hua Li, Yue Wei, Xiao‐ming Zhang, Li Zhang","doi":"10.1111/pbi.70271","DOIUrl":"https://doi.org/10.1111/pbi.70271","url":null,"abstract":"According to the Food and Agriculture Organization of the United Nations (FAO), pests reduce global crop production by 14% annually. The growing challenge of pest resistance, coupled with the relatively low success rates of pesticides, has prompted researchers to shift their attention towards the accurate evaluation of insecticide lead. In contrast to in vitro methods of structural similarity or target affinity, the ‘insecticide‐likeness’ approach emphasises the in vivo biological effects of compounds, thereby constructing precise and comprehensive evaluation rules. In the present study, a multi‐scale qualitative‐quantitative insecticide‐likeness evaluation platform, Agrochem Predictive Platform for Insecticide‐likeness (APPi), was developed. An APPi rule was proposed for qualitative evaluation (ClogP ≤ 7, ARB ≤ 18, HBA ≤ 7, HBD ≤ 2, PFI ≤ 8 and ROB ≤ 10). A quantitative insecticide‐likeness evaluation model, the APPi model, was developed based on a multi‐classifier integrated machine learning framework (PUMV). The APPi model demonstrated excellent performance on the train and external test sets. Crucially, on the independent external test set, it achieved an accuracy of 85%, which represents a significant improvement over existing models. Furthermore, we developed the FragScore Visualiser tool to identify critical insecticidal fragments of compounds. The APPi platform provides precise guidance for virtual screening and structure optimisation of lead compounds in the early stage of insecticides discovery. The platform is available free of charge at <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://pesticides.cau.edu.cn/APPi\">http://pesticides.cau.edu.cn/APPi</jats:ext-link>.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"6 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of an efficient Agrobacterium‐mediated transformation system for chilli pepper and its application in genome editing","authors":"Yaping Tang, Xinyan Shen, Xuan Deng, Yingda Song, Yuhong Zhou, Yongen Lu, Feng Li, Bo Ouyang","doi":"10.1111/pbi.70216","DOIUrl":"https://doi.org/10.1111/pbi.70216","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"52 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Design and Immunogenicity of a Novel Self‐Adjuvanting Mucosal Vaccine Candidate for SARS‐CoV‐2 Expressed in Plants","authors":"Mi‐Young Kim, Andy Cano Tran, Ju Kim, Humblenoble Stembridge Ayuk, Adam Sparrow, Lorenzo Bossi, Megan Brown, Emil Joseph Vergara, Kathrin Göritzer, Elisabetta Groppelli, Tae‐Ho Kwon, Julian K. C. Ma, Yong‐Suk Jang, Rajko Reljic","doi":"10.1111/pbi.70278","DOIUrl":"https://doi.org/10.1111/pbi.70278","url":null,"abstract":"Mucosal vaccination for COVID‐19 to boost preexisting though insufficient systemic and local/mucosal immunity remains an attractive prospect but there are currently no licensed mucosal vaccines against this infection. Here, using a plant expression system, we developed a novel mucosal vaccine platform for respiratory viruses and demonstrated its application in the context of SARS‐CoV‐2 infection. In addition to the antigen itself, the PCF (Platform CTB‐Fc) vaccine candidate incorporates two molecular adjuvants, the IgG‐Fc antibody fragment and the nontoxic cholera toxin B subunit (CTB), with the first targeting the vaccine to IgG receptors on antigen‐presenting cells, and the second providing local adjuvanticity by targeting cellular gangliosides in the mucosae. We demonstrated that this vaccine candidate is highly immunogenic in mice, inducing virus‐neutralising systemic and mucosal antibodies as well as tissue resident memory T cells in the lungs. We also demonstrated that SRBD‐PCF is recognised by immune cells from exposed or vaccinated individuals, and that circulating antibodies also bind to the antigen within the vaccine, forming immune complexes (IC). Finally, with a view of respiratory delivery, we demonstrated that the vaccine can be aerosolised without loss of material or biological activity, and that it is noncytotoxic and nonhaemolytic to human cells. Furthermore, we demonstrate that the plant expression system represents a suitable platform to produce these complex, multifunctional macromolecules capable of simultaneously binding to multiple targets. Our data strongly support the case for a safe, self‐adjuvanting mucosal COVID‐19 vaccine development, as means to boosting both systemic and mucosal immunity.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"52 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red Alga Porphyridium Supports High‐Yield Production of a Functional Chimeric Hepatitis B Surface Antigen With Strong Cellular and Humoral Immunogenicity","authors":"Ana‐Maria Pantazica, Alexander Hammel, Iuliana Caras, Irina Ionescu, Catalin Tucureanu, Adrian Onu, Maria Murace, Jihong Liu Clarke, Crina Stavaru, Norica Branza‐Nichita, Ralph Bock","doi":"10.1111/pbi.70270","DOIUrl":"https://doi.org/10.1111/pbi.70270","url":null,"abstract":"Microalgae represent promising production factories for the light‐driven, cost‐effective production of recombinant proteins. The red microalga <jats:styled-content style=\"fixed-case\"><jats:italic>Porphyridium purpureum</jats:italic></jats:styled-content> displays particularly favourable transgene expression properties due to the episomal maintenance of transformation vectors at high copy numbers in the nucleus. In this work, we explored the potential of <jats:styled-content style=\"fixed-case\"><jats:italic>Porphyridium purpureum</jats:italic></jats:styled-content> to synthesise a candidate vaccine against Hepatitis B virus (HBV). We show high‐yield expression of an HBV chimeric surface antigen and efficient assembly of virus‐like particles (VLPs) in algal cells. We established a purification protocol for the VLPs and conducted vaccination studies in experimental animals. The results demonstrate that the alga‐produced HBV antigen elicits superior humoral and cellular immune responses compared to a commercial HBV vaccine produced in yeast. The antigen triggers virus‐neutralising antibodies against different HBV variants, including vaccine‐escape mutations that evade the immune response to current vaccines in humans. Our work establishes <jats:italic>Porphyridium</jats:italic> as a highly promising production platform for vaccines and other proteinaceous biopharmaceuticals.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of DNA Methylation in Peanut Leaves and Roots: Uncovering the Molecular Mechanisms for Increased Yield After Single‐Seed Sowing","authors":"Shikai Fan, Jialei Zhang, Feng Guo, Fangji Xu, Zhaohui Tang, Rongchong Li, Bo Bai, Yiyang Liu, Guowei Li, Shubo Wan","doi":"10.1111/pbi.70264","DOIUrl":"https://doi.org/10.1111/pbi.70264","url":null,"abstract":"Cytosine methylation is a crucial epigenetic modification that responds to various environmental cues, yet the specific mechanisms influencing planting patterns remain incompletely understood. This study reveals significant growth differences between single‐seed (SS) precision sowing and double‐seed (DS) sowing observed 42 days after germination under controlled indoor conditions. These differences were eliminated by the application of the DNA methylation inhibitor 5‐azacytidine (5‐aza), highlighting the role of DNA methylation in these processes. To further investigate the role of DNA methylation in planting pattern, we generated DNA methylation profiles of peanut leaves and roots under both DS and SS planting patterns. The analysis revealed increased CHH methylation in both tissues, caused by the RNA‐directed DNA methylation (RdDM) pathway. Further analysis, including differential methylation, transposable element (TE) analysis and methylation‐related gene analysis, demonstrated tissue‐specific epigenetic responses to planting patterns. Integrating methylome and transcriptome data, we found that DS was associated with hyper‐CHH methylation in WRKY gene promoters in leaves, accelerating leaf senescence. Meanwhile, SS reduced CHH methylation in promoters in roots, upregulating genes involved in flavonoid biosynthesis. This upregulation enhanced root nodule formation and improved stress resistance, resulting in increased concentrations of nitrogen and phosphorus in the roots, as confirmed by metagenomic functional analysis. This research provides novel insights into the epigenetic regulation of plant growth and development.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"51 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hypersensitive response‐like lesion‐inducing protein modulates basal immunity against grey leaf spot in maize","authors":"Tao Zhong, Chenyu Guo, Suining Deng, Mang Zhu, QianQian Zhang, Mingliang Xu","doi":"10.1111/pbi.70239","DOIUrl":"https://doi.org/10.1111/pbi.70239","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"47 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon‐positive photorespiratory bypass via the tartronyl‐coenzyme A pathway enhances carbon fixation efficiency and yield in rice","authors":"Guoxin Chen, Kaining Jin, Jingke Wang, Yanni Li, Xinhua Tian, Liying Zhang, Suting Wu, Jinwen Yang, Xuean Cui, Jing Sun, Xuehui Sun, Tiegang Lu, Zhiguo Zhang","doi":"10.1111/pbi.70258","DOIUrl":"https://doi.org/10.1111/pbi.70258","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gigas‐Cell1 mediated in vivo haploid induction in Brassica napus: A step forward for hybrid development and crop improvement","authors":"Muhammad Zeeshan Mola Bakhsh, Mengyu Lei, Xiaoyu Zhang, Ahmad Ali, Bin Yi","doi":"10.1111/pbi.70215","DOIUrl":"https://doi.org/10.1111/pbi.70215","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"100 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural variation of the wheat root exudate metabolome and its influence on biological nitrification inhibition activity.","authors":"Arindam Ghatak,Alexandros E Kanellopoulos,Cristina López-Hidalgo,Andrea Malits,Yuhang Meng,Florian Schindler,Shuang Zhang,Jiahang Li,Steffen Waldherr,Hugo Ribeiro,Melina Kerou,Logan H Hodgskiss,Maximilian Dreer,Reyazul Rouf Mir,Sandeep Sharma,Gert Bachmann,Dimitrios G Karpouzas,Christa Schleper,Evangelia S Papadopoulou,Palak Chaturvedi,Wolfram Weckwerth","doi":"10.1111/pbi.70248","DOIUrl":"https://doi.org/10.1111/pbi.70248","url":null,"abstract":"Excessive nitrogen use and low nitrogen use efficiency (NUE) in current agroecosystems are disrupting the global nitrogen cycle. Chemical inhibitors offer only temporary relief, while plant-derived biological nitrification inhibitors (BNIs) remain safer but underexplored. Identifying biological nitrification inhibition (BNI) traits in nitrogen-demanding crops like wheat is key to improving sustainability. In this study, a combined GC- and LC-MS platform was used to determine the metabolome of the root exudates of 44 diverse wheat genotypes originating from India and Austria. With more than 6000 metabolic features, a pronounced genotype-specific variation, a clear geographic pattern and an unexpected complexity of the root exudate metabolome were observed. A novel high-throughput assay utilizing diverse ammonia-oxidizing bacteria (AOB) and archaea (AOA) was developed for rapid BNI testing, highlighting distinct inhibition and even growth stimulation capacities between genotypes. Network analysis and advanced machine and deep learning analysis identified combinations of 32 metabolites linked to high BNI activity, including phenylpropanoids sinapinic acid, syringic acid and others, as well as glycosylated flavones isoschaftoside and others. This indicates that the concurrent presence of specific metabolites, rather than a single compound, drives nitrification inhibition in the rhizosphere. Variation in BNI activity among wheat genotypes, classified as either spring or winter types, suggests that root architecture modulates the dynamics of root exudation and the potential for nitrification inhibition. The unique combination of high-throughput metabolomics analysis and the BNI fast-track assay allows for screening of large germplasm collections as an essential requirement to introduce BNI and related NUE traits into modern breeding programmes.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"10 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}