Plant Biotechnology Journal最新文献

{"title":"LRM3 positively regulates stem lodging resistance by degradating MYB6 transcriptional repressor in soybean","authors":"Yongheng Ye, Zhiyuan Cheng, Xinjing Yang, Suxin Yang, Kuanqiang Tang, Hui Yu, Jinshan Gao, Yaohua Zhang, Jiantian Leng, Wei Zhang, Ye Zhang, Moran Bu, Zhengwei Liang, Zhicheng Dong, Zhonghui Zhang, Xianzhong Feng","doi":"10.1111/pbi.70124","DOIUrl":"https://doi.org/10.1111/pbi.70124","url":null,"abstract":"SummaryStem lodging resistance plays a critical role in maintaining soybean yield stability, yet the molecular mechanisms governing stem development and lodging tolerance remain poorly understood. Here, we report the characterization of <jats:italic>lodging‐related mutant 3</jats:italic> (<jats:italic>lrm3</jats:italic>), a weak‐stemmed soybean line exhibiting increased lodging susceptibility. Molecular cloning revealed that <jats:italic>LRM3</jats:italic> encodes a U‐box E3 ubiquitin ligase that physically interacts with the transcription factor MYB6, targeting it for 26S proteasome‐mediated degradation. Transcriptomic and chromatin immunoprecipitation analyses demonstrated that MYB6 binds directly to the promoter regions of <jats:italic>PHENYLALANINE AMMONIA‐LYASE</jats:italic> (<jats:italic>PAL</jats:italic>) genes, repressing their transcriptional activity and consequently reducing lignin biosynthesis and secondary cell wall deposition in stems. Population genetic analysis identified three major <jats:italic>LRM3</jats:italic> haplotypes, with Haplotype 1 preferentially retained in landraces and modern cultivars, suggesting artificial selection during domestication. Collectively, our findings elucidate a previously uncharacterized regulatory mechanism integrating ubiquitin‐mediated proteolysis and phenylpropanoid metabolism to enhance stem mechanical strength. This study provides novel genetic insights and molecular tools for improving lodging resistance in soybean breeding programs.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"35 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PGCP: A comprehensive database of plant genomes for comparative phylogenomics.","authors":"Xinkai Zhou,Hai-Yun Fan,Xing-Yu Feng,Zhonghao Ruan,Jinkai Yuan,Quan Han,Zhaohui He,Yuxin You,Haoyu Chao,Ming Chen,Zhu-Qing Shao,Jia-Yu Xue,Dijun Chen","doi":"10.1111/pbi.70110","DOIUrl":"https://doi.org/10.1111/pbi.70110","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"30 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome assembly and population genomic analysis reveal the genetic basis of popcorn evolution","authors":"Xiaojian Fang, Hangqin Liu, Jiacheng Liu, Yang Song, Min Xu, Xing Jian, Li Dong, Qianwen Zhang, Le Xu, Guorui Fan, Zhaoying Wang, Yiwen You, Tianyu Feng, Wenyu Li, Yuling Li, Rentao Song, Zhongwei Lin","doi":"10.1111/pbi.70125","DOIUrl":"https://doi.org/10.1111/pbi.70125","url":null,"abstract":"SummaryPopcorn, one of the world's most popular snack foods, represents the most ancient type of maize domesticated by humans. However, the genetic basis underlying popcorn evolution and kernel‐popping traits remains largely unknown. In this study, we assembled a high‐quality genome sequence of the popcorn landrace <jats:italic>Strawberry Popcorn</jats:italic> (SP) and conducted extensive population genomic analyses. The SP genome spans 2.3 Gb and harbours a large inversion on chromosome 8, along with millions of genetic variants that enable the discovery of beneficial alleles. Translocations and substantial duplications of the <jats:italic>Ga1</jats:italic> gene occurred in the locus associated with unilateral cross‐incompatibility on chromosome 4. Tandemly duplicated <jats:italic>Ga1</jats:italic> genes underwent pseudogenisation and truncation with complete loss of gene function. The <jats:italic>P1</jats:italic> gene experienced gene expansion and regulatory modifications, leading to downregulation of transcription and subsequent loss of pericarp colour during maize domestication and improvement. Population genomic analysis further identified a subset of 12 marker genes from over 2494 genes under human selection, which were reshaped to enhance kernel‐popping traits during domestication. These marker genes include <jats:italic>Pl1</jats:italic> and <jats:italic>Dek1</jats:italic> for pericarp and aleurone layer thickness; <jats:italic>THP9</jats:italic>, <jats:italic>Sh2</jats:italic>, <jats:italic>SUS1</jats:italic>, <jats:italic>Smk10</jats:italic>, <jats:italic>KW1</jats:italic>, <jats:italic>O7</jats:italic>, and <jats:italic>NKD1</jats:italic> for protein and starch biosynthesis; and <jats:italic>VP5</jats:italic>, <jats:italic>CCD7</jats:italic>, and <jats:italic>Crti3</jats:italic> for carotene biosynthesis, which all influence endosperm vitreousness, a key factor determining kernel hardness for popping. Among these genes, <jats:italic>KW1</jats:italic> and <jats:italic>O7</jats:italic> stand out as pivotal genes with a significant impact on kernel‐popping performance. These results provide a wealth of gene targets to greatly accelerate the molecular breeding of improved popcorn varieties.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"46 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fijivirus capsid protein hijacks autophagy degrading an ω‐3 fatty acid desaturase to suppress jasmonate‐mediated antiviral defence","authors":"Jianjian Liu, Xinxin Jing, Pengyue Wang, Gaohua Wang, Meirong Xiang, Pengbai Li, Hongfeng Zou, Honglian Li, Zujian Wu, Chaonan Wang, Songbai Zhang, Chao Zhang","doi":"10.1111/pbi.70119","DOIUrl":"https://doi.org/10.1111/pbi.70119","url":null,"abstract":"SummaryPlant viruses often suppress jasmonic acid (JA)‐mediated defences through disturbing JA signalling or biosynthesis pathways to benefit their own infection. Few studies have examined how the precursors of JA biosynthesis are regulated by viral infection. In this study, we demonstrate that rice black‐streaked dwarf virus (RBSDV) infection inhibits the production of α‐linolenic acid (C18:3), a key JA biosynthesis precursor that is catalysed by a set of fatty acid desaturases (FADs). The viral capsid protein P10 directly interacts with OsFAD7, an ω‐3 fatty acid desaturase, and promotes its autophagic degradation through an ATG8‐interaction motif (AIM). This disrupts JA production and weakens antiviral defence against RBSDV infection. Genetic analysis reveals that overexpression of <jats:italic>OsFAD7</jats:italic> enhances JA levels and resistance to virus. But OsFAD7‐mediated antiviral resistance is attenuated if <jats:italic>OsCOI1a</jats:italic>, a JA receptor, is silenced, indicating that the enhancement of resistance to RBSDV infection conferred by OsFAD7 depends on the JA pathway. Our findings reveal a novel viral strategy that suppresses JA biosynthesis at its metabolic source, providing insights for developing viral protection strategies and virus‐resistant crops.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoter replacement by genome editing creates gain‐of‐function traits in Arabidopsis","authors":"Takashi Nobusawa, Michiharu Nakano, Yumi Nagashima, Makoto Kusaba","doi":"10.1111/pbi.70123","DOIUrl":"https://doi.org/10.1111/pbi.70123","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"56 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsFON879, an orphan gene, regulates floral organ homeostasis in rice","authors":"Dong‐Hui Wang, Zhi‐Hong Xu, Shu‐Nong Bai","doi":"10.1111/pbi.70121","DOIUrl":"https://doi.org/10.1111/pbi.70121","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"139 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A telomere‐to‐telomere gap‐free assembly integrating multi‐omics uncovers the genetic mechanism of fruit quality and important agronomic trait associations in pomegranate","authors":"Lina Chen, Hao Wang, Tingtao Xu, Ruitao Liu, Juanli Zhu, Haoxian Li, Huawei Zhang, Liying Tang, Dan Jing, Xuanwen Yang, Qigao Guo, Peng Wang, Luwei Wang, Junhao Liu, Shuyun Duan, Zhaoning Liu, Mengchi Huang, Xiaolong Li, Zhenhua Lu","doi":"10.1111/pbi.70107","DOIUrl":"https://doi.org/10.1111/pbi.70107","url":null,"abstract":"SummaryPomegranate is an important perennial fruit tree distributed worldwide. Reference genomes with gaps and limit gene identification controlling important agronomic traits hinder its functional genomics and genetic improvements. Here, we reported a telomere‐to‐telomere (T2T) gap‐free genome assembly of the distinctive cultivar ‘Moshiliu’. The Moshiliu reference genome was assembled into eight chromosomes without gaps, totalling ~366.71 Mb, with 32 158 predicted protein‐coding genes. All 16 telomeres and eight centromeres were characterized; combined with FISH analysis, we revealed the atypical telomere units in pomegranate as TTTTAGGG. Furthermore, a total of 16 loci associated with 15 important agronomic traits were identified based on GWAS of 146 accessions. Gene editing and biochemical experiments demonstrated that a 37.2‐Kb unique chromosome translocation disrupting the coding domain sequence of <jats:italic>PgANS</jats:italic> was responsible for anthocyanin‐less, knockout of <jats:italic>PgANS</jats:italic> in pomegranate exhibited a defect in anthocyanin production; a unique repeat expansion in the promoter of <jats:italic>PgANR</jats:italic> may affected its expression, resulting in black peel; notably, the G → A transversion located at the 166‐bp coding domain of <jats:italic>PgNST3</jats:italic>, which caused a E56K mutation in the PgNST3 protein, closely linked with soft‐seed trait. Overexpression of <jats:italic>PgNST3</jats:italic><jats:sup><jats:italic>A</jats:italic></jats:sup> in tomato presented smaller and softer seed coats. The E56K mutation in PgNST3 protein, eliminated the binding ability of PgNST3 to the <jats:italic>PgMYB46</jats:italic> promoter, which subsequently affected the thickness of the inner seed coat of soft‐seeded pomegranates. Collectively, the validated gap‐free genome, the identified genes controlling important traits and the CRISPR‐Cas9‐mediated gene knockout system all provided invaluable resources for pomegranate precise breeding.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BrCNGC12 and BrCNGC16 mediate Ca2+ absorption and transport to enhance resistance to tipburn in Chinese cabbage","authors":"Jingping Yuan, Changwei Shen, Ruixiang Chen, Yunduan Qin, Shuai Li, Bo Sun, Chunyang Feng, Xinlei Guo","doi":"10.1111/pbi.70113","DOIUrl":"https://doi.org/10.1111/pbi.70113","url":null,"abstract":"SummaryTipburn is a common physiological disorder in leafy vegetables, significantly impairing crop growth and commercial value. It is widely recognized that Ca<jats:sup>2+</jats:sup> deficiency is a key factor triggering tipburn; however, the functions and regulatory mechanisms of genes conferring resistance remain largely unexplored. Through transcriptomic analysis of Chinese cabbage under normal (medium calcium, MCa) and Ca<jats:sup>2+</jats:sup>‐deficient (low calcium, LCa) conditions, we observed that genes in the hormone and calcium signalling pathways exhibited significant responses to LCa stress. Among these, the cyclic nucleotide‐gated ion channel (<jats:italic>CNGC</jats:italic>) genes <jats:italic>BrCNGC12</jats:italic> and <jats:italic>BrCNGC16</jats:italic>, part of the calcium signalling pathway, were notably up‐regulated and down‐regulated, respectively, under LCa stress. Silencing BrCNGC12 in Chinese cabbage improves Ca<jats:sup>2+</jats:sup> absorption and distribution, which strengthens tipburn resistance. Conversely, under LCa stress, heterologous expression of <jats:italic>BrCNGC16</jats:italic> in <jats:italic>Arabidopsis thaliana</jats:italic> increases resistance to tipburn, whereas partial silencing of <jats:italic>BrCNGC16</jats:italic> in Chinese cabbage diminishes resistance, with both outcomes linked to altered Ca<jats:sup>2+</jats:sup> uptake and translocation. Additionally, overexpression of <jats:italic>BrCNGC16</jats:italic> in Chinese cabbage promotes Ca<jats:sup>2+</jats:sup> uptake and translocation, thereby enhancing resistance to tipburn and mitigating oxidative damage induced by Ca<jats:sup>2+</jats:sup> deficiency. In conclusion, <jats:italic>BrCNGC12</jats:italic> and <jats:italic>BrCNGC16</jats:italic> play pivotal roles in tipburn resistance in Chinese cabbage, offering novel insights into the interplay between the calcium signalling pathway and tipburn resistance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"25 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High yield production of the antifungal proteins PeAfpA and PdAfpB by vacuole targeting in a TMV‐based expression vector","authors":"Adrià Bugeda, Xiaoqing Shi, Laia Castillo, Jose F. Marcos, Paloma Manzanares, Juan José López‐Moya, María Coca","doi":"10.1111/pbi.70093","DOIUrl":"https://doi.org/10.1111/pbi.70093","url":null,"abstract":"SummaryAntifungal proteins (AFPs) derived from filamentous fungi show great potential against economically significant fungi that cause plant diseases and consequently threat food safety and security. This study focuses on the <jats:italic>Penicillium expansum</jats:italic> PeAfpA and <jats:italic>Penicillium digitatum</jats:italic> PdAfpB proteins and their activity against several phytopathogens. The AFPs were synthesized through a highly productive tobacco mosaic virus‐based expression vector in the fast‐growing model plant <jats:italic>Nicotiana benthamiana</jats:italic>, combining signalling sequences for apoplastic and vacuolar compartmentalization to increase yields. Adding a vacuolar signalling peptide from a <jats:italic>Nicotiana sylvestris</jats:italic> chitinase at the C‐termini of the AFPs in combination with an apoplastic N‐terminal signalling peptide from <jats:italic>N. benthamiana</jats:italic> osmotin significantly enhanced AFP yields without altering functionality. Results showed an improvement of ninefold for PeAfpA and 3,5‐fold for PdAfpB compared to constructs with only the apoplastic N‐terminal signalling. Transmission electron microscopy and immunogold labelling confirmed the localization of AFPs in both the apoplast and the vacuole, highlighting its compatibility with vacuolar environments. <jats:italic>In vitro</jats:italic> and <jats:italic>in vivo</jats:italic> assessments against key pathogenic fungi, including <jats:italic>Magnaporthe oryzae</jats:italic>, <jats:italic>Botrytis cinerea</jats:italic> and <jats:italic>Fusarium proliferatum</jats:italic>, revealed that the activities of easily purified PeAfpA‐ and PdAfpB‐enriched plant extracts closely mirrored those of their purified fungal counterparts. This innovative approach represents a notable advance towards the application of AFPs as effective, safe and environmentally friendly ‘green biofungicides’ for safeguarding crop and postharvest produce and could also be applied to control other pathogenic fungi that threat human health.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"72 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A MYB61-SWB9-KOs module regulates grain chalkiness via gibberellin biosynthesis in rice endosperm","authors":"Yujuan Chen, Suozhen Hui, Huijuan Li, Guiai Jiao, Ruijie Cao, Liang Zhou, Jingxin Wang, Amos Musyoki Mawia, Lingwei Yang, Yu Wu, Yuanyaun Zhang, Zhonghua Sheng, Gaoneng Shao, Fengli Zhao, Ling Wang, Yusong Lyu, Shaoqing Tang, Shikai Hu, Peisong Hu","doi":"10.1111/pbi.70103","DOIUrl":"https://doi.org/10.1111/pbi.70103","url":null,"abstract":"Grain chalkiness leads to the deterioration of grain appearance quality, which affects grain processing quality and the market value of rice. Gibberellin plays a crucial role in seed germination and plant growth, but its mechanism on endosperm starch synthesis and rice grain chalkiness formation remains largely elusive. Here, we identified a grain white belly (chalkiness in the belly area of grain) gene, <i>SWB9</i>, which encodes a kinesin-4 protein with a conserved ATPase domain and a coiled-coil domain. The mutation of <i>SWB9</i> affects the starch structure, resulting in a grain white belly. <i>SWB9</i> regulates endogenous gibberellin synthesis and accumulation in endosperm by directly binding to the promoter of ent-kaurene oxidase genes (<i>KO1, KO2 and KOL5</i>) encoding gibberellin-biosynthetic enzymes, and negatively regulates their expression. The loss of <i>SWB9</i> function resulted in higher gibberellin content in the endosperm of <i>swb9</i> than that of the wild type. Besides, a MYB transcription factor, MYB61 binds to the promoter of <i>SWB9</i> and activates its expression. The grain of <i>myb61</i> showed the same white belly phenotype as <i>swb9,</i> while overexpression of <i>SWB9</i> in <i>myb61</i> inhibited the grain white belly phenotype. Furthermore, the exogenous GA₃ treatment showed increased grain chalkiness, and high gibberellin treatment can induce the reduced expression of <i>MYB61</i>, and then weaken the inhibitory effect of SWB9 on the expression of <i>KO1, KO2 and KOL5</i>, so as to break the homeostasis of endogenous gibberellin in the endosperm. Meanwhile, MYB61 directly binds to the promoter of amylopectin synthesis-related genes, <i>SSIIa</i>, <i>BEIIb</i>, <i>ISA1</i> and <i>PUL,</i> at the GAMYB element and activates their expression, further affecting the distribution of amylopectin chain length. Our findings uncover a new insight into the gibberellin dose-dependent feedback regulation loop in rice endosperm that determines grain chalkiness formation.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143885267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}