Plant Biotechnology Journal最新文献_第5页

High‐Efficiency and Eco‐Friendly Management of Multiple Fungal Diseases by a Novel Carbon Dots sCDP Delivered RNA Nano‐Pesticides 新型碳点sCDP递送RNA纳米农药对多种真菌病害的高效环保治理

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-23 DOI: 10.1111/pbi.70378

Chunhao Jiang, Jijie Cui, Yumeng Wang, Ziyi Zhang, Yi Yang, Tao Tang, Junjie Wang, Daijing Li, Zihan Zhao, Kuaibing Wang, Dongdong Niu

{"title":"High‐Efficiency and Eco‐Friendly Management of Multiple Fungal Diseases by a Novel Carbon Dots sCDP Delivered RNA Nano‐Pesticides","authors":"Chunhao Jiang, Jijie Cui, Yumeng Wang, Ziyi Zhang, Yi Yang, Tao Tang, Junjie Wang, Daijing Li, Zihan Zhao, Kuaibing Wang, Dongdong Niu","doi":"10.1111/pbi.70378","DOIUrl":"https://doi.org/10.1111/pbi.70378","url":null,"abstract":"RNA‐based pesticides represent a transformative approach in agricultural disease management, offering an environmentally sustainable alternative to conventional chemical fungicides. However, key challenges such as rapid RNA degradation in the environment and inefficient uptake hinder widespread adoption. Nanocarriers have the potential to significantly enhance RNA delivery efficiency and stability, which holds great promise for developing eco‐friendly strategies in crop protection. Here, we developed a novel multicomponent nano‐biopesticide platform utilising novel polyethyleneimine‐modified carbon dots (sCDP) for dsRNA delivery. It was demonstrated that sCDP forms stable electrostatic complexes with dsRNA (sCDP‐dsRNA), conferring dual protective advantages and enhanced RNA stability with extended foliar persistence exceeding 14 days. Moreover, the sCDP can significantly reduce the contact angle of dsRNA on the leaf surface, and the complexation of sCDP with dsRNA remarkably enhances uptake efficiency into plant leaves and pathogens' mycelium, thereby enhancing plant protection of RNA pesticides. Additionally, sCDP exhibits good biocompatibility and low toxicity against pathogens and plants. Finally, it was demonstrated that sCDP‐dsRNA complexes showed significant efficacy in preventing and treating rice sheath blight, both in laboratory and field conditions. Moreover, the platform demonstrated broad‐spectrum antifungal activity against four major pathogens including Aspergillus niger, Botrytis cinerea, Magnaporthe oryzae, and Phakopsora pachyr under different conditions. Thus, this nanoplatform establishes an efficient and environmentally sustainable crop protection paradigm by synergizing the precision of RNA interference with enhanced delivery capabilities.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"8 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice MGD1‐Mediated Improvement of Photosynthesis and Crop Yield by Flexible Adaptation to Different Light Conditions 水稻MGD1介导的对不同光照条件的灵活适应对光合作用和作物产量的改善

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-23 DOI: 10.1111/pbi.70380

Shasha Li, Patrick J. Horn, Meijuan Zhang, Guanqiang Zuo, Yi Wang, Xiping Deng, Lina Yin, Shiwen Wang

{"title":"Rice MGD1‐Mediated Improvement of Photosynthesis and Crop Yield by Flexible Adaptation to Different Light Conditions","authors":"Shasha Li, Patrick J. Horn, Meijuan Zhang, Guanqiang Zuo, Yi Wang, Xiping Deng, Lina Yin, Shiwen Wang","doi":"10.1111/pbi.70380","DOIUrl":"https://doi.org/10.1111/pbi.70380","url":null,"abstract":"Flexible adaptation to different light intensities found in natural environments is crucial for efficient photosynthesis and yield production in crops. The ability to cope with suboptimal light conditions effectively and efficiently is clearly advantageous. In this study, increased photosynthetic ability, biomass accumulation, grain yield and high membrane lipid contents were observed in OsMGD1‐overexpression plants of rice and tobacco under both low and high light conditions. Further exploration of the photosynthetic performance and xanthophyll cycle‐dependent photoprotection in these transgenic plants revealed that under low light conditions, the overexpression lines maintained high levels of chlorophyll content and light harvesting capability, leading to a high photosynthetic quantum yield. While under high light conditions, the de‐epoxidation status of xanthophyll cycle pigments was higher in the overexpression plants, leading to sufficient photoprotection and reduced photo‐oxidative damage, resulting in an increased electron transport rate. These results indicate that OsMGD1 is involved in regulating photosynthetic processes when plants are exposed to different light intensities, providing an effective strategy for achieving improved photosynthesis and crop production under variable light conditions in nature.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"79 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosmarinic Acid Synthase 1 Phosphorylation by SmMAPK3 Is Required for Salicylic Acid‐Induced Salvianolic Acid Accumulation in Salvia miltiorrhiza Hairy Roots SmMAPK3磷酸化迷迭香酸合成酶1是水杨酸诱导丹参毛状根中丹酚酸积累的必要条件

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-23 DOI: 10.1111/pbi.70370

Xuecui Yin, Shuang Liu, Yuhang Zhang, Wenjing Yang, Junwen Bu, Bin Zhang, Juane Dong

{"title":"Rosmarinic Acid Synthase 1 Phosphorylation by SmMAPK3 Is Required for Salicylic Acid‐Induced Salvianolic Acid Accumulation in Salvia miltiorrhiza Hairy Roots","authors":"Xuecui Yin, Shuang Liu, Yuhang Zhang, Wenjing Yang, Junwen Bu, Bin Zhang, Juane Dong","doi":"10.1111/pbi.70370","DOIUrl":"https://doi.org/10.1111/pbi.70370","url":null,"abstract":"Salvianolic acid is the main active component of Salvia miltiorrhiza and holds significant value in the clinical treatment of myocardial ischemia and hypoxia. Previous studies have shown that salicylic acid (SA) can significantly promote the accumulation of salvianolic acid, but its molecular mechanism remains incompletely understood. To reveal the molecular mechanism by which SA regulates salvianolic acid, this study treated transgenic hairy roots overexpressing mitogen‐activated protein kinase 3 (SmMAPK3) with SA and demonstrated that SmMAPK3 is essential for SA‐induced salvianolic acid biosynthesis, with this function relying on the kinase activity of SmMAPK3. Through yeast two‐hybrid screening, Rosmarinic Acid Synthase 1 (SmRAS1) was identified as a direct interacting protein of SmMAPK3. In vitro kinase assays showed that SmMAPK3 can catalyse phosphorylation of SmRAS1 at the Ser178 site, a process critical for salvianolic acid biosynthesis. Further studies confirmed that SA promotes the interaction between SmMAPK3 and SmRAS1, activates the kinase activity of SmMAPK3, and enhances the phosphorylation level and protein stability of SmRAS1. Additionally, SA treatment significantly promoted salvianolic acid accumulation in transgenic hairy roots coexpressing SmRAS1 and SmMAPK3. This study elucidates the mechanism by which the SA‐SmMAPK3‐SmRAS1 signalling module regulates salvianolic acid biosynthesis through post‐translational modification, providing new theoretical targets for metabolic engineering and yield improvement of S. miltiorrhiza.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"41 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EBSn, a Robust Synthetic Reporter for Monitoring Ethylene Responses in Plants. 监测植物乙烯反应的合成报告基因EBSn。

IF 10.5 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-21 DOI: 10.1111/pbi.70302

Josefina-Patricia Fernandez-Moreno, Mario Fenech, Anna E Yaschenko, Chengsong Zhao, Matthew Neubauer, Hannah N Davis, Alex J Marchi, Raine Concannon, Alexandra Keren-Keiserman, Moshe Reuveni, Victor Levitsky, Dmitry Oshchepkov, Vladislav Dolgikh, Alexander Goldshmidt, José T Ascencio-Ibáñez, Elena Zemlyanskaya, Jose M Alonso, Anna N Stepanova

{"title":"EBSn, a Robust Synthetic Reporter for Monitoring Ethylene Responses in Plants.","authors":"Josefina-Patricia Fernandez-Moreno, Mario Fenech, Anna E Yaschenko, Chengsong Zhao, Matthew Neubauer, Hannah N Davis, Alex J Marchi, Raine Concannon, Alexandra Keren-Keiserman, Moshe Reuveni, Victor Levitsky, Dmitry Oshchepkov, Vladislav Dolgikh, Alexander Goldshmidt, José T Ascencio-Ibáñez, Elena Zemlyanskaya, Jose M Alonso, Anna N Stepanova","doi":"10.1111/pbi.70302","DOIUrl":"10.1111/pbi.70302","url":null,"abstract":"Ethylene is a gaseous plant hormone that controls a wide array of physiologically relevant processes, including plant responses to biotic and abiotic stress, and induces ripening in climacteric fruits. To monitor ethylene in plants, analytical methods, phenotypic assays, gene expression analysis and transcriptional or translational reporters are typically employed. In the model plant Arabidopsis, two ethylene-sensitive synthetic transcriptional reporters have been described, 5xEBS:GUS and 10x2EBS-S10:GUS. These reporters harbour a different type, arrangement and number of homotypic cis-elements in their promoters and thus may recruit the ethylene master regulator EIN3 in the context of alternative transcriptional complexes. Accordingly, the patterns of GUS activity in these transgenic lines differ and neither of them encompasses all plant tissues even in the presence of saturating levels of exogenous ethylene. Herein, we set out to develop and test a more sensitive version of the ethylene-inducible promoter that we refer to as EBSnew (abbreviated as EBSn). EBSn leverages a tandem of 10 non-identical, natural copies of a novel, dual, everted, 11 bp-long EIN3-binding site, 2EBS(-1). We show that in Arabidopsis, EBSn outperforms its predecessors in terms of its ethylene sensitivity, having the capacity to monitor endogenous levels of ethylene and displaying more ubiquitous expression in response to the exogenous hormone. We demonstrate that the EBSn promoter is also functional in tomato, opening new avenues to manipulating ethylene-regulated processes, such as ripening and senescence, in crops.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants 基于双病毒的模块化植物高货量表达和基因靶向载体的开发

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-21 DOI: 10.1111/pbi.70320

Matthew Neubauer, Katie Vollen, Chengsong Zhao, José T. Ascencio-Ibáñez, Linda Hanley-Bowdoin, Anna N. Stepanova, Jose M. Alonso

{"title":"Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants","authors":"Matthew Neubauer, Katie Vollen, Chengsong Zhao, José T. Ascencio-Ibáñez, Linda Hanley-Bowdoin, Anna N. Stepanova, Jose M. Alonso","doi":"10.1111/pbi.70320","DOIUrl":"https://doi.org/10.1111/pbi.70320","url":null,"abstract":"Viral vectors can be useful tools for expressing recombinant proteins as well as delivering gene-editing machinery. Despite their utility, the development and subsequent optimisation of these tools is often a difficult and tedious process. Thus, although considerable work has been done to create useful viral vectors for gene editing and protein expression, there is a lack of understanding of how best to design these vectors for specific applications. For instance, it is often unclear whether the inclusion of heterologous promoter sequences or different viral components will improve cargo expression or replicon accumulation. To address some of these hurdles, we designed a GoldenBraid (GB)-compatible viral vector system based on the geminivirus—beet curly top virus (BCTV). This system allows for simple, modular cloning of a variety of reporter constructs. Making use of this modular cloning strategy, we compared a variety of alternative viral vector architectures. Interestingly, native BCTV promoters outperformed the constitutive 35S promoter, while the removal of the BCTV virion-sense genes promoted reporter expression. Intriguingly, these modifications had no effect on total replicon accumulation. These results show the utility of the new modular BCTV-based vectors for protein expression and gene targeting applications, as well as uncover design principles that may inform future geminivirus-based viral vector architectures. We anticipate that the availability of this new modular system will spark the broad adoption of replicon-based strategies in protein expression and gene editing experiments in plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"39 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strigolactones Regulate Sugar Allocation to Control Rice Tillering and Root Development via the OsSPL14-OsSHR1-OsSWEET16 Pathway. 寡糖内酯通过OsSPL14-OsSHR1-OsSWEET16通路调控水稻分蘖和根系发育

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-19 DOI: 10.1111/pbi.70374

Miao Feng,Wenfan Luo,Sheng Luo,Rong Miao,Manpo Gu,Shuai Li,Xinxin Xing,Jinhui Zhang,Jinsheng Qian,Xin Liu,Chunlei Zhou,Qi Sun,Tong Luo,Nuo Chen,Yulong Ren,Zhijun Cheng,Cailin Lei,Zhichao Zhao,Shanshan Zhu,Xin Wang,Xiuping Guo,Qibing Lin,Jianmin Wan

{"title":"Strigolactones Regulate Sugar Allocation to Control Rice Tillering and Root Development via the OsSPL14-OsSHR1-OsSWEET16 Pathway.","authors":"Miao Feng,Wenfan Luo,Sheng Luo,Rong Miao,Manpo Gu,Shuai Li,Xinxin Xing,Jinhui Zhang,Jinsheng Qian,Xin Liu,Chunlei Zhou,Qi Sun,Tong Luo,Nuo Chen,Yulong Ren,Zhijun Cheng,Cailin Lei,Zhichao Zhao,Shanshan Zhu,Xin Wang,Xiuping Guo,Qibing Lin,Jianmin Wan","doi":"10.1111/pbi.70374","DOIUrl":"https://doi.org/10.1111/pbi.70374","url":null,"abstract":"Strigolactones (SLs) are root-to-shoot phytohormones that regulate tillering (branching) and root development. Sugar, as an essential energy substance and signalling molecule, plays a fundamental role in the growth and development of plants. However, the molecular mechanisms by which SL directly regulates sugar allocation to control tillering and root development are still not fully understood. Here, we found that OsSHR1 operates directly downstream of OsSPL14 within the SL signalling pathway, facilitating root elongation while inhibiting tillering and crown root development. The expression of OsSHR1 is stimulated by OsSPL3/12/14 in vivo, and D53 can interact with these SPL proteins to suppress their transcriptional activities. Interestingly, we further demonstrate that OsSHR1 can directly bind to the promoters of OsSWEET2a/4/16, which encode sugar transporters that can control the allocation of sugar in plant growth and development. This binding facilitates the expression of sugar transporters, which in turn regulate sugar allocation and enable the plant's response to SLs. The results indicate that via the OsSPL14-OsSHR1-OsSWEET16 pathway, SLs orchestrate the distribution of sugars to ensure their effectiveness in stimulating root elongation while simultaneously suppressing tillering and crown root formation in rice.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"54 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145089758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heritable, Tissue Culture-Independent and Transgene-Free Genome Editing in Plants via Viral Delivery of CRISPR/AsCas12f. 通过病毒传递CRISPR/AsCas12f在植物中进行可遗传、不依赖组织培养和无转基因的基因组编辑

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-19 DOI: 10.1111/pbi.70315

Manman Hu,Lingran Zhang,Luis Herrera-Estrella,Degao Liu

引用次数: 0

Domestication-Selected Promoter Insertion in WRKY17 Increases Cadmium Sensitivity in Apple. WRKY17启动子插入增加苹果镉敏感性

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-19 DOI: 10.1111/pbi.70376

Chanyu Wang,Tianchao Wang,Tong Zhang,Handong Song,Dongqian Shan,Yixue Bai,Zehui Hu,Jianyu Li,Jie Man,Peiyun Xiao,Changjian Dai,Xin Liu,Fei Shen,Jin Kong,Jianfang Hu,Xiaodong Zheng

{"title":"Domestication-Selected Promoter Insertion in WRKY17 Increases Cadmium Sensitivity in Apple.","authors":"Chanyu Wang,Tianchao Wang,Tong Zhang,Handong Song,Dongqian Shan,Yixue Bai,Zehui Hu,Jianyu Li,Jie Man,Peiyun Xiao,Changjian Dai,Xin Liu,Fei Shen,Jin Kong,Jianfang Hu,Xiaodong Zheng","doi":"10.1111/pbi.70376","DOIUrl":"https://doi.org/10.1111/pbi.70376","url":null,"abstract":"With increasing industrialisation and human activities, heavy metal pollution has become a serious environmental concern, particularly cadmium (Cd) contamination. This study reveals significant differences in Cd tolerance between wild apple (Malus spp.) and cultivated apple (Malus domestica). Through pan-genome analysis, we identified the transcription factor WRKY17 as a key regulator of Cd stress response, with a 3355-bp insertion (P-INS) in its promoter region being the primary genetic basis for this differential tolerance. In cultivated apples, P-INS suppresses WRKY17 expression, leading to reduced Cd tolerance. In contrast, wild apples lacking P-INS exhibit activated WRKY17 expression. Further investigation demonstrated that WRKY17 enhances Cd tolerance by inducing the expression of long non-coding RNA lncRNA400. Mechanistically, lncRNA400 forms an R-loop structure that recruits the histone demethylase JMJD5 to remove H3K27me3 marks from the promoter of the Plant Cadmium Resistance gene PCR2, thereby activating PCR2 expression. Notably, WRKY17 activation also accelerates leaf senescence, explaining why P-INS was retained during apple domestication-its suppression of WRKY17 maintains better agronomic traits despite reduced Cd tolerance. In apple cultivation, grafting wild apple rootstocks with cultivated scions effectively combines the Cd-tolerant traits of wild varieties with the delayed leaf senescence characteristics of cultivated cultivars, providing a practical solution for the apple industry to address Cd contamination.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"78 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145083317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Diversity of the DNA-Binding Landscape in the DREB/ERF Family: Focusing on Reproductive Processes in Fruit Trees With Highly Heterozygous Genome. DREB/ERF家族dna结合景观的多样性——以基因组高度杂合果树的生殖过程为中心

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-18 DOI: 10.1111/pbi.70375

Fengqi Wu,Jiakun Zheng,Huimin Hu,Hongsen Liu,Yaxuan Xiao,Junting Feng,Yanwei Hao,Chengjie Chen,Rui Xia,Zaohai Zeng

{"title":"The Diversity of the DNA-Binding Landscape in the DREB/ERF Family: Focusing on Reproductive Processes in Fruit Trees With Highly Heterozygous Genome.","authors":"Fengqi Wu,Jiakun Zheng,Huimin Hu,Hongsen Liu,Yaxuan Xiao,Junting Feng,Yanwei Hao,Chengjie Chen,Rui Xia,Zaohai Zeng","doi":"10.1111/pbi.70375","DOIUrl":"https://doi.org/10.1111/pbi.70375","url":null,"abstract":"DREB/ERF transcription factors play pivotal roles in plant development; however, their structural characteristics, DNA-binding preferences, and functional roles in highly heterozygous woody plants remain insufficiently understood. Using lychee (Litchi chinensis) as a model, we identified 95 DREB/ERF genes subdivided into ten phylogenetic groups. DNA affinity purification sequencing (DAP-seq) of 45 representative members uncovered 65 194 binding sites with subfamily-specific motifs: C(G/A)CCG(A/C)C for DREB and CGCCG(C/T)C for ERF subfamilies. Each group exhibited unique binding motif preferences, aligning with their protein structures and essential peptide positions. Notably, LITCHI017494 directly regulated terpenoid biosynthesis and aroma formation by activating tandemly repeated LcTPS genes. Furthermore, single nucleotide polymorphisms (SNPs) in LITCHI017494's binding sites altered the binding efficiency of two flowering-related genes (LcSVP and LcVOZ) in early- and late-maturing haplotypes, revealing a mechanism underlying flowering and fruit maturation period. Overall, with experimental evidence, this study provides a comprehensive binding profile of the DREB/ERF family in lychee, revealing intricate transcriptional regulatory networks and serving as a crucial resource for transcription factor research within complex genomic contexts, especially in the DREB/ERF gene family.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"29 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145078229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucuronoyl Esterase of Pathogenic Phanerochaete carnosa Induces Immune Responses in Aspen Independently of Its Enzymatic Activity. 致病性肉芽平毛菌葡萄糖醛酸酯酶诱导杨树免疫应答的独立酶活性。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-09-17 DOI: 10.1111/pbi.70357

Evgeniy N Donev,Marta Derba-Maceluch,Xiao-Kun Liu,Henri Colyn Bwanika,Izabela Dobrowolska,Mohit Thapa,Joanna Leśniewska,Jan Šimura,Alex Yi-Lin Tsai,Konrad S Krajewski,Dan Boström,Leszek A Kleczkowski,Maria E Eriksson,Karin Ljung,Emma R Master,Ewa J Mellerowicz

{"title":"Glucuronoyl Esterase of Pathogenic Phanerochaete carnosa Induces Immune Responses in Aspen Independently of Its Enzymatic Activity.","authors":"Evgeniy N Donev,Marta Derba-Maceluch,Xiao-Kun Liu,Henri Colyn Bwanika,Izabela Dobrowolska,Mohit Thapa,Joanna Leśniewska,Jan Šimura,Alex Yi-Lin Tsai,Konrad S Krajewski,Dan Boström,Leszek A Kleczkowski,Maria E Eriksson,Karin Ljung,Emma R Master,Ewa J Mellerowicz","doi":"10.1111/pbi.70357","DOIUrl":"https://doi.org/10.1111/pbi.70357","url":null,"abstract":"Microbial enzymes expressed in plants add new functionalities but occasionally trigger undesirable immune responses. Phanerochaete carnosa glucuronoyl esterase (PcGCE) hydrolyses the bond between lignin and 4-O-methyl-α-D-glucuronic acid substituent of glucuronoxylan. PcGCE constitutively expressed in Arabidopsis or hybrid aspen (Populus tremula × tremuloides) improved saccharification but also induced premature leaf senescence. To understand what triggered this senescence, we characterised PcGCE-expressing hybrid aspen by microscopy and omics approaches, supplemented by grafting and recombinant protein application experiments. PcGCE induced massive immune responses followed by senescence in the leaves. Expressing an inactive (PcGCES217A) enzyme has led to similar phenotypes, excluding a possibility that damage-associated molecular patterns (DAMPs) released by glucuronoyl esterase triggered immune responses. Grafting experiments showed that PcGCE transcripts are not mobile but they induce systemic responses. Recombinant PcGCE protein applied to leaves did not induce such responses; thus, PcGCE is probably not perceived as a pathogen-associated molecular pattern (PAMP). We suggest that the observed high expression of PcGCE from the 35S promoter triggers the unfolded protein response. Indeed, restricting PcGCE expression to short-lived xylem cells by using the wood-specific promoter avoided all detrimental effects. Thus, wood-specific expression is a viable strategy for PcGCE deployment in planta, which might be applicable for other stress-inducing proteins.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"35 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145071776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0