Plant Biotechnology Journal最新文献

{"title":"Production of grains with low glutelin and high eating quality by using dominant allele Lgc-1 in three-line japonica hybrid rice","authors":"Shikai Hu, Lingwei Yang, Jinyang Cai, Guiai Jiao, Hailong Yang, Suozhen Hui, Liang Zhou, Ruijie Cao, Jingxin Wang, Yujuan Chen, Junchao Fang, Zhonghua Sheng, Shaoqing Tang, Peisong Hu","doi":"10.1111/pbi.14502","DOIUrl":"10.1111/pbi.14502","url":null,"abstract":"<p>Grain proteins constitute the second most storage substance in rice, of which glutelin accounts for 60%–80% of total protein and is easy to be absorbed by humans (Kumamaru <i>et al</i>., <span>1988</span>). However, for patients with kidney disease and diabetes, excessive glutelin intake is not conducive to recovery. The <i>lgc-1</i> mutant is the earliest discovered low-glutelin material, and <i>Lgc-1</i> regulates glutelin content in rice grains (Iida <i>et al</i>., <span>1993</span>; Kusaba <i>et al</i>., <span>2003</span>), which makes this allele have more extensive application prospects in the cultivation of low-glutelin varieties. Rice eating quality (REQ) is influenced by protein content and composition. Studies have shown that with the increase in protein content, the REQ decreases and the palatability becomes worse (Huang <i>et al</i>., <span>2020</span>). Exogenous glutelin and prolamin could affect REQ, whereas knockout of glutelin-related genes could significantly improve the hardness, appearance and REQ. Therefore, the effect of glutelin on REQ may be greater than prolamin and total protein (Furukawa <i>et al</i>., <span>2006</span>; Huang <i>et al</i>., <span>1998</span>; Yang <i>et al</i>., <span>2022</span>). Furthermore, it was found that the expression of genes related to glutelin synthesis had an important effect on protein content and REQ. As the expression of <i>Nhd1</i> increased, the expression of <i>GluA2</i> was inhibited, resulting in the decrease of glutelin content and protein content, thus improving REQ (Zhang <i>et al</i>., <span>2023</span>). These studies indicated that glutelin can significantly affect REQ, however, fewer studies have been done on japonica hybrid rice with high eating quality and low glutelin.</p><p>Combining with molecular marker and phenotypic screening, three low-glutelin restorer lines, HL8005, HL8023 and HL8027, were screened by crossing two varieties L9037 and R228 (Figure 1a). L9037 is a low-glutelin variety with the genotype <i>Lgc-1</i> (without restoration gene), and R228 is a wide compatibility restorer line (without genotype <i>Lgc-1</i>). The amplified bands of HL8005, HL8023 and HL8027 were consistent with L9037 by molecular markers (Figure 1c; Figure S1). Compared with L9037, the number of grains per panicle decreased by 4.0% for HL8005, increased by 11.8% and 32.2% for HL8023 and HL8027, respectively, and the 1000-grain weight increased by 4.1% and 9.2% for HL8005 and HL8023, respectively, while the 1000-grain weight of HL8027 decreased by 4.6%, and the seed setting rate were all above 75% (Figure 1b). The heading time of three restorer lines was significantly shorter than L9037, and the single plant yield and population yield of three restorer lines were significantly higher than L9037 (Figure S2). The glutelin content of HL8005, HL8023 and HL8027 were significantly lower than R228, but higher than L9037 (Figure 1d). SDS-PAGE of storage profiles showed that the protein composi","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"23 2","pages":"374-376"},"PeriodicalIF":10.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.14502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring intra- and intergenomic variation in haplotype-resolved pangenomes","authors":"Eef M. Jonkheer, Dick de Ridder, Theo A. J. van der Lee, Jorn R. de Haan, Lidija Berke, Sandra Smit","doi":"10.1111/pbi.14545","DOIUrl":"https://doi.org/10.1111/pbi.14545","url":null,"abstract":"With advances in long-read sequencing and assembly techniques, haplotype-resolved (phased) genome assemblies are becoming more common, also in the field of plant genomics. Computational tools to effectively explore these phased genomes, particularly for polyploid genomes, are currently limited. Here we describe a new strategy adopting a pangenome approach. To analyse both intra- and intergenomic variation in phased genome assemblies, we have made the software package PanTools ploidy-aware by updating the pangenome graph representation and adding several novel functionalities to assess synteny and gene retention, profile repeats and calculate synonymous and nonsynonymous mutation rates. Using PanTools, we constructed and analysed a pangenome comprising of one diploid and four tetraploid potato cultivars, and a pangenome of five diploid apple species. Both pangenomes show high intra- and intergenomic allelic diversity in terms of gene absence/presence, SNPs, indels and larger structural variants. Our findings show that the new functionalities and visualizations are useful to discover introgressions and detect likely misassemblies in phased genomes. PanTools is available at https://git.wur.nl/bioinformatics/pantools.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"3 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mirages in continuous directed enzyme evolution: a cautionary case study with plantized bacterial THI4 enzymes","authors":"Kristen Van Gelder, Anuran K. Gayen, Andrew D. Hanson","doi":"10.1111/pbi.14563","DOIUrl":"https://doi.org/10.1111/pbi.14563","url":null,"abstract":"<p>Continuous directed evolution (CDE) improves the characteristics of a target enzyme by hypermutating the enzyme gene <i>in vivo</i>, coupling enzyme activity to growth of a microbial platform, and selecting for growth rate (Molina <i>et al</i>., <span>2022</span>). Directed evolution can be interfaced with genome editing to expand the gene pool available for plant breeding; this powerful combination (DE–GE) has been neatly termed ‘a Green (r)Evolution’ (Gionfriddo <i>et al</i>., <span>2019</span>). THI4 enzymes, which make the thiazole moiety of thiamin, are good testbed targets for plant CDE technology. Plant THI4s are energy-inefficient suicide enzymes that could potentially be replaced by efficient, non-suicide bacterial THI4s to increase biomass yield by as much as 4% (Joshi <i>et al</i>., <span>2021</span>). However, bacterial THI4s are O<sub>2</sub>-sensitive and otherwise ill-adapted to plants (Joshi <i>et al</i>., <span>2021</span>). We therefore previously ran CDE campaigns in the yeast OrthoRep system to ‘plantize’ bacterial THI4s, that is, to improve function in an aerobic, plant-like milieu (Figure 1a) (García-García <i>et al</i>., <span>2022</span>). Two notably successful campaigns were for the THI4 from <i>Mucinivorans hirudinis</i> (MhTHI4); these campaigns culminated when populations acquired single V124A or Y122C mutations that improved growth to near the wild-type rate (Van Gelder <i>et al</i>., <span>2023</span>). Such culmination can be overcome by increasing the selection pressure (Molina <i>et al</i>., <span>2022</span>).</p>\u0000<figure><picture>\u0000<source media=\"(min-width: 1650px)\" srcset=\"/cms/asset/5d1a35f3-b3df-4cfa-bd4e-d6fe7c98b2d5/pbi14563-fig-0001-m.jpg\"/><img alt=\"Details are in the caption following the image\" data-lg-src=\"/cms/asset/5d1a35f3-b3df-4cfa-bd4e-d6fe7c98b2d5/pbi14563-fig-0001-m.jpg\" loading=\"lazy\" src=\"/cms/asset/bd12af5d-8049-40c0-b660-06ab303db600/pbi14563-fig-0001-m.png\" title=\"Details are in the caption following the image\"/></picture><figcaption>\u0000<div><strong>Figure 1<span style=\"font-weight:normal\"></span></strong><div>Open in figure viewer<i aria-hidden=\"true\"></i><span>PowerPoint</span></div>\u0000</div>\u0000<div>OrthoRep campaigns to plantize a bacterial THI4 and their outcomes. (a) The OrthoRep system. The target enzyme (MhTHI4 V124A), plus or minus a poly(A) tail, is encoded on the cytoplasmic p1 plasmid that also carries a LEU2 marker. p1 is hypermutated by a p1-specific, error-prone DNA polymerase (TP-DNAP1_611 or TP-DNAP1_633) encoded on a nuclear plasmid. The BY4741 platform strain carries a <i>thi4</i>Δ deletion to couple growth to the activity of the THI4 on p1. (b) The combinations of expression-reduction regimes with cold turkey (CT) or gradual (G) selection. (c) The non-synonymous (blue) and synonymous (green) mutations that had swept populations by the end of campaigns. When populations failed to grow early in campaigns, surviving populations were split into subpopulations (A, B, C) and prop","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"23 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142917510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of nucleus‐encoded trans‐acting factors allows orthogonal fine‐tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii","authors":"Pawel Mateusz Mordaka, Kitty Clouston, Aleix Gorchs‐Rovira, Catherine Sutherland, Daniel Qingyang Zhang, Katrin Geisler, Payam Mehrshahi, Alison Gail Smith","doi":"10.1111/pbi.14557","DOIUrl":"https://doi.org/10.1111/pbi.14557","url":null,"abstract":"SummaryThe green microalga <jats:italic>Chlamydomonas reinhardtii</jats:italic> is a promising host organism for the production of valuable compounds. Engineering the <jats:italic>Chlamydomonas</jats:italic> chloroplast genome offers several advantages over the nuclear genome, including targeted gene insertion, lack of silencing mechanisms, potentially higher protein production due to multiple genome copies and natural substrate abundance for metabolic engineering. Tuneable expression systems can be used to minimize competition between heterologous production and host cell viability. However, complex gene regulation and a lack of tight regulatory elements make this a challenge in the <jats:italic>Chlamydomonas</jats:italic> chloroplast. In this work, we develop two synthetic tuneable systems to control the expression of genes on the chloroplast genome, taking advantage of the properties of the vitamin B<jats:sub>12</jats:sub>‐responsive <jats:italic>METE</jats:italic> promoter and a modified thiamine (vitamin B<jats:sub>1</jats:sub>) riboswitch, along with nucleus‐encoded chloroplast‐targeted regulatory proteins NAC2 and MRL1. We demonstrate the capacity of these systems for robust, fine‐tuned control of several chloroplast transgenes, by addition of nanomolar levels of vitamins. The two systems have been combined in a single strain engineered to avoid effects on photosynthesis and are orthogonal to each other. They were then used to manipulate the production of an industrially relevant diterpenoid, casbene, by introducing and tuning expression of the coding sequence for casbene synthase, as well as regulating the metabolite flux towards casbene precursors, highlighting the utility of these systems for informing metabolic engineering approaches.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"90 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142888069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel transcription factor OsMYB73 affects grain size and chalkiness by regulating endosperm storage substances' accumulation‐mediated auxin biosynthesis signalling pathway in rice","authors":"Song Liu, Jiamin Wu, Amos Musyoki Mawia, Xiangjin Wei, Ruijie Cao, Guiai Jiao, Yawen Wu, Jian Zhang, Lihong Xie, Zhonghua Sheng, Shikai Hu, Sanfeng Li, Yusong Lv, Feifei Lu, Yujuan Chen, Sajid Fiaz, Javaria Tabassum, Zhimin Du, Fangyuan Gao, Guangjun Ren, Gaoneng Shao, Peisong Hu, Shaoqing Tang","doi":"10.1111/pbi.14558","DOIUrl":"https://doi.org/10.1111/pbi.14558","url":null,"abstract":"SummaryEnhanced grain yield and quality traits are everlasting breeding goals. It is therefore of great significance to uncover more genetic resources associated with these two important agronomic traits. Plant MYB family transcription factors play important regulatory roles in diverse biological processes. However, studies on genetic functions of MYB in rice yield and quality are rarely to be reported. Here, we investigated a nucleus‐localized transcription factor <jats:italic>OsMYB73</jats:italic> which is preferentially expressed in the early developing pericarp and endosperm. We generated targeted mutagenesis of <jats:italic>OsMYB73</jats:italic> in rice, and the mutants had longer grains with obvious white‐belly chalky endosperm appearance phenotype. The mutants displayed various changes in starch physicochemical characteristics and lipid components. Transcriptome sequencing analysis showed that <jats:italic>OsMYB73</jats:italic> was chiefly involved in cell wall development and starch metabolism. <jats:italic>OsMYB73</jats:italic> mutation affects the expression of genes related to grain size, starch and lipid biosynthesis and auxin biosynthesis. Moreover, inactivation of <jats:italic>OsMYB73</jats:italic> triggers broad changes in secondary metabolites. We speculate that rice <jats:italic>OsMYB73</jats:italic> and <jats:italic>OsNF‐YB1</jats:italic> play synergistic pivotal role in simultaneously as transcription activators to regulate grain filling and storage compounds accumulation to affect endosperm development and grain chalkiness through binding <jats:italic>OsISA2</jats:italic>, <jats:italic>OsLTPL36</jats:italic> and <jats:italic>OsYUC11</jats:italic>. The study provides important germplasm resources and theoretical basis for genetic improvement of rice yield and quality. In addition, we enriches the potential biological functions of rice MYB family transcription factors.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"62 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142888137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modification of Fc-fusion protein structures to enhance efficacy of cancer vaccine in plant expression system","authors":"Sohee Lim, Hyun Joo Chung, Yoo Jin Oh, Peter Hinterdorfer, Soon Chul Myung, Young-Jin Seo, Kisung Ko","doi":"10.1111/pbi.14552","DOIUrl":"https://doi.org/10.1111/pbi.14552","url":null,"abstract":"Epithelial cell adhesion molecule (EpCAM) fused to IgG, IgA and IgM Fc domains was expressed to create IgG, IgA and IgM-like structures as anti-cancer vaccines in <i>Nicotiana tabacum</i>. High-mannose glycan structures were generated by adding a C-terminal endoplasmic reticulum (ER) retention motif (KDEL) to the Fc domain (FcK) to produce EpCAM-Fc and EpCAM-FcK proteins in transgenic plants via <i>Agrobacterium</i>-mediated transformation. Cross-fertilization of EpCAM-Fc (FcK) transgenic plants with Joining chain (J-chain, J and JK) transgenic plants led to stable expression of large quaternary EpCAM-IgA Fc (EpCAM-A) and IgM-like (EpCAM-M) proteins. Immunoblotting, SDS–PAGE and ELISA analyses demonstrated that proteins with KDEL had higher expression levels and binding activity to anti-EpCAM IgGs. IgM showed the strongest binding among the fusion proteins, followed by IgA and IgG. Sera from BALB/c mice immunized with these vaccines produced anti-EpCAM IgGs. Flow cytometry indicated that the EpCAM-Fc fusion proteins significantly activated CD8⁺ cytotoxic T cells, CD4⁺ helper T cells and B cells, particularly with EpCAM-FcK^P and EpCAM-Fc^P (FcK^P) × J^P (JK^P). The induced anti-EpCAM IgGs captured human prostate cancer PC-3 and colorectal cancer SW620 cells. Sera from immunized mice inhibited cancer cell proliferation, migration and invasion; down-regulated proliferation markers (PCNA, Ki-67) and epithelial–mesenchymal transition markers (Vimentin); and up-regulated E-cadherin. These findings suggest that <i>N. tabacum</i> can produce effective vaccine candidates to induce anti-cancer immune responses.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"127 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142887239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the Editor-in-Chief","authors":"Johnathan Napier","doi":"10.1111/pbi.14560","DOIUrl":"10.1111/pbi.14560","url":null,"abstract":"<p>As 2024 draws to a close, it once again provides an opportunity to recognize and thank everyone who has contributed to the continued success of <i>Plant Biotechnology Journal</i>, a combined effort from the Editors, the reviewers and the Wiley team. I deeply appreciate the diligence and hard work everyone puts into helping authors elevate and improve their manuscripts, ultimately providing useful additions to the published literature.</p><p><i>Plant Biotechnology Journal</i> continues to be a top-ranked journal, maintaining its position in the top five titles in the Plant Sciences category. Our Editors have handled over 1300 submissions so far this year, and we are on track to publish ~250 papers in 2024. It is fair to say that the PBJ team and I are very proud of our metrics and rankings (Impact Factor 11.2 and CiteScore 20.5). Perhaps, a more impressive number is for article downloads, which for 2024 was ~2 million—to my mind, that really does demonstrate relevance and utility. In 2024, PBJ also provided sponsorship to a diverse range of scientific meetings, helping to build communities and forge new connections. A personal highlight for me was the recent meeting in Samson, Turkey entitled <i>Agricultural Biotechnology in the Era of Genome Editing</i> which was organized by AAB and supported by PBJ. I was incredibly impressed with the science presented at this congress as well as the enthusiasm of the participants—I felt lucky to be part of it.</p><p>Given the global challenges that we face, the importance of discovery and innovation is ever more apparent and necessary. Although the massive disruption of Covid-19 now seems like a distant memory, it is important to recall that it was scientific innovation (in the form of new vaccines) that stemmed the pandemic. Now we face probably even greater, more complex threats in the form of accelerating climate change and the associated disruption to environments and food production. I believe that plant biotechnology (and PBJ as part of that vibrant community) can play a key role in mitigating these perturbations, and I would encourage everyone to consider how their knowledge and expertise might contribute solutions to these global challenges. Plant biotechnology has delivered amazing disruptive agricultural innovations at a massive scale (such as herbicide-tolerant crops), and I believe that it can and will do so again in response to the climate crisis. In that respect, PBJ will continue to play a role, publishing the best and most useful advances in our field.</p><p>Best wishes for 2025,</p><p>Johnathan</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"23 1","pages":"3"},"PeriodicalIF":10.1,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.14560","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel geminivirus‐derived 3′ flanking sequence of terminator mediates the gene expression enhancement","authors":"Yi Zhang, Yibo Xian, Heng Yang, Xuangang Yang, Tianli Yu, Sai Liu, Minting Liang, Xianzhi Jiang, Shulin Deng","doi":"10.1111/pbi.14561","DOIUrl":"https://doi.org/10.1111/pbi.14561","url":null,"abstract":"SummaryExploring the new elements to re‐design the expression cassette is crucial in synthetic biology. Viruses are one of the most important sources for exploring gene expression elements. In this study, we found that the DNA sequence of the SBG51 deltasatellite from the <jats:italic>Sweet potato leaf curl virus</jats:italic> (SPLCV) greatly enhanced the gene expression when flanked downstream of the terminator. The SBG51 sequence increased transient <jats:italic>GFP</jats:italic> gene expression in <jats:italic>Nicotiana benthamiana</jats:italic> leaves by up to ~6 times and ~10 times compared to the gene expression controlled by the UBQ10 promoter and 35S promoter alone, respectively. The increased <jats:italic>GFP</jats:italic> gene expression level contributed to the continuous accumulation of GFP protein and GFP fluorescence until 8 days post‐inoculation (dpi). The SBG51 sequence also enhanced the gene expression in the transgenic Arabidopsis plants and maintained the spatio‐temporal pattern of the <jats:italic>FLOWERING LOCUS T</jats:italic> (<jats:italic>FT</jats:italic>) and <jats:italic>TOO MANY MOUTHS</jats:italic> (<jats:italic>TMM</jats:italic>) promoters. We identified a 123 bp of AT‐rich sequence containing seven “ATAAA” or “TTAAA” elements from the SBG51 DNA, which had the gene expression enhancement effect. Furthermore, the artificial synthetic sequences containing tandem repeated “ATAAA” or “TTAAA” elements were sufficient to increase the gene expression but did not alter the polyadenylation of mRNA, similar to the function of matrix attachment regions (MAR). Additionally, the compact artificial synthetic sequence also had an effect on yeast when the expression cassette was integrated into the genome. We conclude that the geminivirus deltasatellite‐derived sequence and the “ATAAA”/“TTAAA” elements are powerful tools for enhancing gene expression.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome of root celery and population genomic analysis reveal the complex breeding history of celery","authors":"Enhui Lai, Sumin Guo, Pan Wu, Minghao Qu, Xiaofen Yu, Chenlu Hao, Shan Li, Haixu Peng, Yating Yi, Miao Zhou, Guodong Fu, Xingnuo Li, Huan Liu, Yi Zheng, Xin Wang, Zhangjun Fei, Lei Gao","doi":"10.1111/pbi.14551","DOIUrl":"https://doi.org/10.1111/pbi.14551","url":null,"abstract":"SummaryCelery (<jats:italic>Apium graveolens</jats:italic> L.) is an important vegetable crop in the Apiaceae family. It comprises three botanical varieties: common celery with solid and succulent petioles, celeriac or root celery with enlarged and fleshy hypocotyls and smallage or leaf celery with slender, leafy and usually hollow petioles. Here we present a chromosome‐level genome assembly of a celeriac cultivar and a comprehensive genome variation map constructed through resequencing of 177 representative celery accessions. Phylogenetic analysis revealed that smallage from the Mediterranean region represented the most ancient type of cultivated celery. Following initial domestication in this region, artificial selection has primarily aimed at enlarging the hypocotyl, resulting in celeriac, and at solidifying the petiole, leading to common celery. Selective sweep analysis and genome‐wide association study identified several genes associated with hypocotyl expansion and revealed that the hollow/solid petiole trait directly correlated with the presence/absence of a NAC gene. Our study elucidates the complex breeding history of celery and provides valuable genomic resources and molecular insights for future celery improvement and conservation efforts.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"76 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial microorganisms: Regulating growth and defense for plant welfare","authors":"Yan Liu, Aiqin Shi, Yue Chen, Zhihui Xu, Yongxin Liu, Yanlai Yao, Yiming Wang, Baolei Jia","doi":"10.1111/pbi.14554","DOIUrl":"https://doi.org/10.1111/pbi.14554","url":null,"abstract":"Beneficial microorganisms (BMs) promote plant growth and enhance stress resistance. This review summarizes how BMs induce growth promotion by improving nutrient uptake, producing growth-promoting hormones and stimulating root development. How BMs enhance disease resistance and help protect plants from abiotic stresses has also been explored. Growth-defense trade-offs are known to affect the ability of plants to survive under unfavourable conditions. This review discusses studies demonstrating that BMs regulate growth-defense trade-offs through microbe-associated molecular patterns and multiple pathways, including the leucine-rich repeat receptor-like kinase pathway, abscisic acid signalling pathway and specific transcriptional factor regulation. This multifaceted relationship underscores the significance of BMs in sustainable agriculture. Finally, the need for integration of artificial intelligence to revolutionize biofertilizer research has been highlighted. This review also elucidates the cutting-edge advancements and potential of plant-microbe synergistic microbial agents.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}