Acta Biomaterialia最新文献

{"title":"Corrigendum to “Photoacoustic processing of decellularized extracellular matrix for biofabricating living constructs” [Acta Biomaterialia 183, 2024, 74-88]","authors":"Luis P. Ferreira, Carole Jorge, Matilde R. Lagarto, Maria V. Monteiro, Iola F. Duarte, Vitor M. Gaspar, João F. Mano","doi":"10.1016/j.actbio.2025.06.052","DOIUrl":"10.1016/j.actbio.2025.06.052","url":null,"abstract":"","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 739-740"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An endoscopy deliverable hydrogel dry powder for sealing and repairing gastric perforation","authors":"Panxianzhi Ni ,&nbsp;Dengbinpei Duan ,&nbsp;Dan Luo ,&nbsp;Mengyi Deng ,&nbsp;Meng Zhong ,&nbsp;Can Huang ,&nbsp;Hongwei He ,&nbsp;Jing Shan ,&nbsp;Tun Yuan ,&nbsp;Jie Liang ,&nbsp;Yujiang Fan ,&nbsp;Xingdong Zhang","doi":"10.1016/j.actbio.2025.08.056","DOIUrl":"10.1016/j.actbio.2025.08.056","url":null,"abstract":"<div><div>Gastric perforation (GP) is characterized by full-thickness injury of the stomach wall, a severe and potentially life-threatening gastrointestinal disease. However, current treatment, including surgical sutures and endoscopic closure, faces limitations, achieving complete sealing of the perforation and favorable healing remains a great challenge and an acute clinical demand. Here, we report a hydrogel dry powder (PPCL@Mg) for the minimally invasive treatment of GP, which can be delivered to target perforation wounds by spraying via an endoscope, and rapidly absorbing interfacial water and spontaneously forming a hydrogel. This hydrogel was designed with strong adhesion, gastric fluid resistance, good anti-swelling behavior, biocompatibility, anti-oxidation, anti-inflammatory and hemostatic properties, which forms instant, robust and sutureless sealing of GP. In vivo rat GP model results indicated that PPCL@Mg hydrogel effectively sealed the perforation and accelerated healing by promoting cell proliferation and angiogenesis, and regulating inflammation and oxidative stress, and the therapeutic effect surpassed that of surgical suture. Furthermore, the large animal pig GP model further validated the repair efficacy of the PPCL@Mg powder and revealed its superior sealing and repair efficacy compared to titanium clips. Transcriptome sequencing and proteomics analysis further verified the significant therapeutic outcomes and elucidated its mechanism in promoting perforation healing. The proposed PPCL@Mg hydrogel dry powder provides a promising therapeutic approach for GP repair and demonstrates significant clinical translational potential.</div></div><div><h3>Statement of Significance</h3><div>As one of the most severe gastrointestinal emergencies, gastric perforation (GP) poses a significant clinical challenge, where reliable perforation sealing and healing are urgently needed. In this work, we introduce PPCL@Mg, a hydrogel dry powder for minimally invasive GP treatment. Delivered endoscopically via spraying, it rapidly absorbs water and spontaneously forms an adhesive hydrogel. Engineered with strong adhesion, gastric-fluid resistance, anti-swelling, biocompatibility, anti-oxidation, anti-inflammatory and hemostatic properties, this hydrogel enables instant, robust, sutureless GP sealing. In vivo rodent and porcine models demonstrated PPCL@Mg hydrogel's efficacy in sealing gastric perforations and accelerating healing. This dry powder system presents a promising strategy for GP repair with significant translational potential.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 270-285"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144982029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The porcine abattoir blood model — Bridging the gap between human and porcine blood for in-vitro testing","authors":"Maike Schönborn,&nbsp;Ilona Mager,&nbsp;Ulrich Steinseifer,&nbsp;Michael Neidlin,&nbsp;Johanna C. Clauser","doi":"10.1016/j.actbio.2025.08.037","DOIUrl":"10.1016/j.actbio.2025.08.037","url":null,"abstract":"<div><h3>Background:</h3><div>Reliable in-vitro thrombogenicity testing of medical devices requires large blood volumes, which cannot be obtained from humans. Slaughterhouse blood is an ethically acceptable, cost-effective alternative. While porcine blood is already used in hemolysis testing, its use for thrombogenicity testing remains limited regarding its comparability to human blood.</div></div><div><h3>Objectives:</h3><div>This study systematically characterizes porcine slaughterhouse in comparison to human blood regarding key parameters relevant for thrombogenicity testing. The goal is to evaluate its suitability for standardized in-vitro tests.</div></div><div><h3>Methods:</h3><div>Human donor and porcine slaughterhouse blood were analyzed for key thrombogenicity parameters. These included coagulation markers (ROTEM parameters, TAT, fibrinogen), platelet and complement activation (PLAs, P-selectin, C3a, SC5b9), and stress-related hormones (adrenaline, cortisol). The influence of two different anticoagulants (enoxaparin and citrate) was also assessed.</div></div><div><h3>Results:</h3><div>Despite moderate pre-activation in pig blood – especially for platelets, complement and stress markers – intact reaction patterns were observed. Exemplarily, ROTEM analysis revealed species-specific characteristics such as reduced clotting time (CT) and increased clot strength (A30) in pigs, while overall reactivity was preserved. In addition, porcine platelets maintained their responsiveness to agonists, suggesting that further activation is possible despite the increased baseline. Importantly, the effect of anticoagulants (citrate vs. enoxaparin) was the same in both species, suggesting that the methodological scope used in in-vitro assays is transferable.</div></div><div><h3>Conclusions:</h3><div>These results emphasize that, when properly collected and handled, slaughterhouse porcine blood can be a viable and reliable alternative to human blood for thrombogenicity testing, although certain baseline differences must be taken into account.</div><div><em>Statement of significance</em>:</div><div>Reliable in-vitro testing of the thrombogenicity of medical devices is crucial for their safety, but requires large volumes of blood that often cannot be covered by conventional human blood donations. This study provides for the first time a comprehensive characterization of pig slaughterhouse blood compared to human blood with regard to hematological, inflammatory and coagulation-related parameters. The results show which properties are comparable and which differences need to be taken into account. The work thus lays the foundation for the establishment of an ethically justifiable and practical thrombogenicity model — an important step towards animal-free thrombogenicity tests in the development of blood-contacting medical products.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 334-345"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144982054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in antisenescence biomaterials for improved osteoarthritis therapy","authors":"Yang-Shuo Ge ,&nbsp;Jia-Ying Ding ,&nbsp;Jun Shen ,&nbsp;Ting-Ting Meng ,&nbsp;Chun-Meng Huang ,&nbsp;Wen-Yao Li ,&nbsp;Min-Jun Zhao ,&nbsp;Jian-li Yin ,&nbsp;Yu-Qing Zhai ,&nbsp;Xue-Zong Wang ,&nbsp;Jian-Guang Xu ,&nbsp;Wenguo Cui ,&nbsp;Dao-Fang Ding","doi":"10.1016/j.actbio.2025.08.044","DOIUrl":"10.1016/j.actbio.2025.08.044","url":null,"abstract":"<div><div>Osteoarthritis (OA) is a degenerative joint disease closely associated with aging for which current treatments are limited primarily to symptomatic relief and fail to reverse pathological progression. A growing body of evidence indicates that the accumulation of senescent cells is a central driver of OA pathogenesis. This review systematically summarizes the latest advancements in antisenescence biomaterials for OA therapy, emphasizing their potential to overcome the limitations of conventional approaches by improving drug targeting, prolonging drug release kinetics, and increasing bioavailability. We categorize these biomaterials on the basis of their antisenescence mechanism and delivery platform, including polymeric nanoparticles (NPs), nanozymes, lipid nanoparticles (LNPs), extracellular vesicles (EVs), hydrogels, and composite hydrogels. These systems have demonstrated efficacy in selectively eliminating senescent cells through various molecular mechanisms such as modulating mitochondrial dysfunction, inflammatory signaling, DNA damage, mechanical overload and autophagy. Furthermore, this review proposes innovative strategies that integrate cellular reprogramming, organelle-targeted therapy, the artificial intelligence (AI)-guided design of antisenescence materials, and stem cell/organoid technologies to address challenges such as barriers to drug delivery and the heterogeneity of the aging microenvironment. Although these biomaterials still require further validation regarding safety, scalability, and regulatory approval for their clinical translation, antisenescence biomaterials represent multidimensional and sustainable therapeutic options for OA and hold promise for reshaping the therapeutic landscape of degenerative joint diseases by targeting the root causes of tissue degeneration.</div></div><div><h3>Statement of significance</h3><div>Osteoarthritis (OA) is an age-related joint disease for which current therapies provide only symptom relief without halting progression. Increasing evidence shows that senescent cells drive OA development. This review summarizes recent advances in antisenescence biomaterials, including nanoparticles, nanozymes, lipid carriers, extracellular vesicles, and hydrogels. These systems improve drug targeting, prolong release, and enhance bioavailability while selectively eliminating senescent cells through mechanisms such as regulating mitochondrial dysfunction, inflammation, and DNA damage. We also highlight innovative approaches integrating cellular reprogramming, organelle-targeted therapy, AI-guided design, and stem cell technologies. Although further validation is required, antisenescence biomaterials offer sustainable strategies that target OA at its root, reshaping future treatment of degenerative joint diseases.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 81-104"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144982080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tunable photoinitiated hydrogel microspheres for quantifying cell-generated forces in complex three-dimensional environments","authors":"Antoni Garcia-Herreros ,&nbsp;Yi-Ting Yeh ,&nbsp;Yunpeng Tu ,&nbsp;Adithan Kandasamy ,&nbsp;Juan C. del Alamo ,&nbsp;Ernesto Criado-Hidalgo","doi":"10.1016/j.actbio.2025.08.041","DOIUrl":"10.1016/j.actbio.2025.08.041","url":null,"abstract":"<div><div>We present a high-throughput method using standard laboratory equipment and microfluidics to produce cellular force microscopy probes with controlled size and elastic modulus. Mechanical forces play crucial roles in cell biology but quantifying these forces in physiologically relevant systems remains challenging due to the complexity of the native cell environment. Polymerized hydrogel microspheres offer great promise for interrogating the mechanics of processes inaccessible to classic force microscopy methods. However, despite significant recent advances, their small size and large surface-to-volume ratio impede the high-yield production of probes with tunable, monodisperse distributions of size and mechanical properties.</div><div>To overcome these limitations, we use a flow-focusing microfluidic device to generate large quantities of droplets with highly reproducible, adjustable radii. These droplets contain acrylamide gel precursor and the photoinitiator Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as a source of free radicals. LAP provides fine control over microsphere polymerization due to its high molar absorptivity at UV wavelengths and moderate water solubility. The polymerized microspheres can be functionalized with different conjugated extracellular matrix proteins and embedded with fluorescent nanobeads to promote cell attachment and track microsphere deformation.</div><div>As proof of concept, we measure the mechanical forces generated by a monolayer of vascular endothelial cells engulfing functionalized microspheres. Individual nanobead motions are tracked and analyzed to determine 3D traction forces via direct computation of stress from measured strain. These results reveal that the cell monolayer collectively exerts strong radial compression on the encapsulated probe, suggesting new biomechanical functions of endothelial cells that could modulate diapedesis or pathogen internalization.</div></div><div><h3>Statement of Significance</h3><div>Mechanical forces are crucial to many cell biology processes but quantifying them in complex native environments remains challenging. We address this by introducing linearly elastic probes with known mechanical properties, whose deformations can be accurately measured to infer local stresses. Specifically, we present a high-throughput method for producing polyacrylamide (PAAm) hydrogel microspheres embedded with fluorescent nanoparticles. To measure cell-generated forces in physiologically relevant systems, the probes are tracked using a 3D coherent point drift algorithm, yielding high-resolution deformation data with minimal computational cost. This method overcomes key barriers in PAAm microsphere fabrication by ensuring monodisperse size, tunable stiffness, and simple, reproducible processes suitable for most cell biology labs—making it a powerful tool for studying cellular mechanobiology.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 521-536"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144982148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aligned hydrogels with continuous gradients for the design of complex bioactive niches","authors":"Huaxiang Yang ,&nbsp;Qiyuan Song ,&nbsp;Yuesheng Huang ,&nbsp;Liying Xiao ,&nbsp;Gongwen Yang ,&nbsp;Qiang Lu","doi":"10.1016/j.actbio.2025.09.008","DOIUrl":"10.1016/j.actbio.2025.09.008","url":null,"abstract":"<div><div>Continuous gradient signals play a vital role in maintaining tissue homeostasis and repairing damaged tissues. A challenge remains for biomaterials to design complex continuous gradients similar to the niches <em>in vivo</em>. Here, a simple but effective strategy is developed to introduce continuous gradient cues to aligned hydrogels by regulating the slow diffusion of nanosized aggregates. Beta-sheet enriched silk nanofibers were tuned to shorter nanoaggregates with ultrasonic treatment to change its diffusion activity. The nanoaggregates were arranged into the pre-designed discontinuous gradient patterns and incubated for several days to convert to continuous gradients through slow diffusion. The low-voltage electrical field was used to stabilize the gradients, following aligned structure formation. The resulting continuous gradients exhibited flexibility, high controllability, and versatility, enabling the formation of multiple complex gradients. <em>S</em>ignificantly better bioactivity was achieved for the hydrogels with continuous gradients, superior to that with discontinuous gradients. The rat full-thickness wound model indicated that the hydrogels with continuous SDF-1<em>α</em> gradients accelerated scarless wound healing and functional recovery, confirming the critical roles of the gradients in tissue regeneration. Our present study provides a universal platform to design complex niches with multiple continuous gradients, opening a new path for regenerative medicine and bionic organoids.</div></div><div><h3>Statement of Significance</h3><div>Both continuous gradient cues and alignment structures play crucial roles in tissue regeneration. Controlled diffusion behaviors were introduced to silk nanofiber systems with pre-designed discontinuous gradients to construct continuous gradients in the aligned hydrogels after electrical field treatment. The biomimetic hydrogels with alignment structure and flexible continuous gradients achieved improved simulation of complex microenvironment in vitro, which effectively regulated cell behaviors and accelerated tissue regeneration. The present work provides a platform to design bioactive materials and study cell-microenvironment interaction.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 319-333"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145056594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in engineering functional cardiac tissues from iPSC-derived cardiomyocytes: advances in construction and applications","authors":"Meiyi Huang ,&nbsp;Sitian Liu ,&nbsp;Xiong Zhou ,&nbsp;Ling Wang ,&nbsp;Yaobin Wu","doi":"10.1016/j.actbio.2025.09.011","DOIUrl":"10.1016/j.actbio.2025.09.011","url":null,"abstract":"<div><div>Engineered cardiac tissue (ECT) has emerged as a transformative platform for modelling cardiac diseases, drug screening, and regenerative therapies. Among the various strategies for ECT construction, cardiomyocytes derived from human induced pluripotent stem cells (iPSC-CMs) have gained prominence due to their capacity to overcome critical limitations of primary cardiomyocyte sources, such as species-specific differences, limited tissue availability, and ethical concerns. In this review, we present a comprehensive overview of recent advancements in the use of iPSC-CMs for ECT development. We begin by outlining current methodologies for differentiating iPSC into cardiomyocytes, followed by an evaluation of key tissue engineering approaches, including scaffold-based, scaffold-free, and biofabrication techniques, that are used to assemble functional cardiac constructs <em>in vitro</em>. Special attention is given to the comparative advantages and challenges of these platforms. We highlight emerging applications of iPSC-CM-based ECTs, focusing on heart-on-a-chip systems for disease modelling and high-throughput drug testing, as well as cardiac patches for myocardial repair. Finally, we highlight major challenges, such as iPSC-CM immaturity, poor vascularization, and limited electromechanical integration, and discuss emerging bioengineering strategies to overcome these barriers and advance the clinical translation of engineered cardiac tissues.</div></div><div><h3>Statement of significance</h3><div>ECT is an increasingly sophisticated platform with significant potential for cardiac disease modelling, drug screening, and regenerative therapy. This review provides a comprehensive analysis of the emerging role of human iPSC-CMs in ECT development, with emphasis on advanced differentiation protocols, biomaterial-guided tissue assembly, and cutting-edge biofabrication strategies. By critically evaluating scaffold-based, scaffold-free, and bioprinting approaches, we offer an integrated perspective on the fabrication of functional cardiac constructs. In addition, we discuss translational applications—including heart-on-a-chip systems and myocardial patches—and examine key challenges such as iPSC-CM immaturity, limited vascularization, and suboptimal electromechanical coupling. This review presents a timely synthesis at the intersection of stem cell biology, biomaterials science, and tissue engineering, intended to guide the design of next-generation therapeutic cardiac tissues.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 141-163"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145056619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles coated with immune cell hybrid membranes for targeted delivery of janus kinase inhibitors and synergistic treatment of autoimmune myocarditis","authors":"Zhenhao Zhang ,&nbsp;Yulong Xiong ,&nbsp;Shangyu Liu,&nbsp;Lishui Shen,&nbsp;Lihui Zheng,&nbsp;Ligang Ding,&nbsp;Lingmin Wu,&nbsp;Limin Liu,&nbsp;Minghao Zhao,&nbsp;Le Li,&nbsp;Zhuxin Zhang,&nbsp;Sheng Su,&nbsp;Xi Peng,&nbsp;Likun Zhou,&nbsp;Mengtong Xu,&nbsp;Yan Yao","doi":"10.1016/j.actbio.2025.09.013","DOIUrl":"10.1016/j.actbio.2025.09.013","url":null,"abstract":"<div><div>Autoimmune myocarditis is a complicated, inflammatory heart disease with high morbidity and mortality. Interferon (IFN)-γ-mediated classical activated macrophage (M1 macrophage) polarization and pyroptosis play a vital role in immune injury in myocarditis. Baricitinib, a selective Janus kinase (JAK) 1 and JAK2 inhibitor, has been used in the treatment of some systemic autoimmune diseases to effectively suppress pro-inflammatory macrophages by blocking the JAK2-signal transducer and activator of transcription 1 (STAT1) signaling pathway. Nevertheless, its application to autoimmune myocarditis was hindered due to the difficulty of delivering and accumulating the drug in heart tissue. To overcome these limitations, we synthesized a hybrid membrane containing CC motif chemokine receptor (CCR) 1 and CXC motif chemokine receptor (CXCR) 3 from activated RAW264.7 and EL4 cell lines to target inflammatory lesions. Furthermore, mesoporous polydopamine (MPDA) was employed due to its synergistic effects, including high drug loading efficiency, reactive oxygen species (ROS) adsorption, and dual responsiveness to glutathione (GSH) and pH, to fabricate RAW-EL4 hybrid membrane-coated Baricitinib-MPDA nanoparticles (BM@[RAW-EL4] NPs) for Baricitinib delivery. Subsequent in vitro and in vivo experiments verified that BM@[RAW-EL4] NPs significantly inhibited inflammatory infiltration and heart tissue injury by precisely suppressing macrophage polarization and pyroptosis. Biotoxicity and biosafety tests also revealed the biocompatibility of BM@[RAW-EL4] NPs, which provided the foundation for further clinical translation. Hence, the biomimetic BM@[RAW-EL4] NPs offer new heart-specific delivery opportunities, representing a versatile platform for targeted therapy in autoimmune myocarditis.</div></div><div><h3>Statement of significance</h3><div>Autoimmune myocarditis is defined as an intense immune injury in the heart tissue, with current treatment far from satisfactory. IFN-γ-mediated M1 macrophage polarization and pyroptosis are crucial to disease progression. In this study, we created RAW-EL4 hybrid membrane-coated Baricitinib-MPDA nanoparticles (BM@[RAW-EL4] NPs) to achieve targeted delivery of an IFN-γ inhibitor to the inflammatory site. RAW-EL4 hybrid membranes endowed the nanomedicine with chemotactic property under the mechanism of activated CCR1-CCL7/8 and CXCR3-CXCL9/10 axis. MPDA exhibited a high drug-loading efficiency of 49.0 % and dual responsiveness to GSH and pH. We also observed its ability to clear ROS in the study. These characteristics of MPDA promoted the release of Baricitinib and macrophage suppression. In vivo experiments revealed the therapeutic effect and biosafety of BM@[RAW-EL4] NPs for the potential application to autoimmune myocarditis.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 584-600"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulated cell death-targeted ocular nanomedicine","authors":"Sok I Ho ,&nbsp;Lin Li ,&nbsp;Sipeng Zuo ,&nbsp;Jieling Tang ,&nbsp;Fuxiang Ye ,&nbsp;Ping Gu ,&nbsp;Zhimin Tang ,&nbsp;Linna Lu","doi":"10.1016/j.actbio.2025.08.018","DOIUrl":"10.1016/j.actbio.2025.08.018","url":null,"abstract":"<div><div>The pathological mechanism of ocular disorders is closely related to dysregulated proliferation or death of ocular cells. Regulated cell death (RCD) is a form of cell death controlled by specific signaling pathways. Diverse types of RCD, such as apoptosis, ferroptosis, autophagy, pyroptosis, and necroptosis, are prevalent in many eye diseases, while the modulation of RCD can alter ocular cell fate and improve disease progression. The pharmacological inducers and inhibitors of RCD have been developed as an emerging approach for ocular therapy. However, the complex ocular anatomy and barriers hinder the efficient use of RCD modulating drugs. Nanomedicine has emerged as a versatile tool in ophthalmic applications for its advanced properties in penetrating ocular barriers, preventing burst effects and rapid inactivation, enabling targeted and controlled release, and facilitating co-delivery of therapeutic agents. Similarly, nanomedicine has been widely explored for its potential in modulating RCD to treat ocular disorders. This review provides a comprehensive overview of the mechanistic roles of five major forms of RCD in eye diseases, summarizes the application of ocular nanomedicine that targets the RCD pathways, and discusses the future prospects of RCD-targeted ocular nanomedicine. It is expected that the elaborately designed RCD-targeted nanomedicine for ocular therapy will play an indispensable role for the establishment of next-generation ocular theranostic nanoplatforms.</div></div><div><h3>Statement of significance</h3><div>Regulated cell death (RCD) significantly manipulates ocular cell fate and is a key driver in the progression of several vision-threatening diseases. However, the complex ocular structures limit the effective use of RCD-regulating therapies. Nanomedicine has emerged as a promising tool to overcome the limitations of the ocular anatomy and precisely modulate RCD, offering new avenues for therapeutic intervention in ocular diseases. In this review, we summarize the mechanistic role of RCD in the pathogenesis of ocular disorders, review current ocular nanomedicines that target the RCD pathway, and discuss the future prospects of RCD-targeted ocular nanomedicine. We aim to provide insights into the potential of targeted nanomedicine in advancing the therapeutics of ocular disorders.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 54-80"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144823387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo degradation behavior of an assembled magnesium alloy suture anchor for ligament-bone reconstruction","authors":"Delin Ma ,&nbsp;Zhaotong Sun ,&nbsp;Qichao Zhao ,&nbsp;Yuan Zhang ,&nbsp;Wancheng Li ,&nbsp;Jie Wang ,&nbsp;Yijing Chen ,&nbsp;Minghui Zhao ,&nbsp;Jun Wang ,&nbsp;Junfei Huang ,&nbsp;Wenxiang Li ,&nbsp;Shijie Zhu ,&nbsp;Liguo Wang ,&nbsp;Xiaochao Wu ,&nbsp;Shaokang Guan","doi":"10.1016/j.actbio.2025.08.019","DOIUrl":"10.1016/j.actbio.2025.08.019","url":null,"abstract":"<div><div>Biodegradable magnesium alloys suture anchors face rapid anchor eyelet degradation, compromising mechanical strength. In this study, an assembled-structure magnesium alloy suture anchor was proposed to mitigate the fast failure of anchor eyelet. <em>In vitro</em> and <em>in vivo</em> experiments were conducted to evaluate the degradation behavior and biomechanical performance of assembled ZE21C magnesium alloy suture anchors. <em>In vitro</em>, mechanical tests revealed stable fixation with a pull-out force of 123.1 ± 5.9 N and fracture strength of 213.3 ± 3.6 N, ensuring no risk of anchor breakage under physiological loads. Immersion in Hanks’ solution demonstrated the screw and tail regions degraded progressively over 14 days, while the anchor eyelet retained structural integrity. The <em>in vivo</em> degradation behavior mirrored <em>in vitro</em> findings and suture anchor maintained its mechanical integrity for 12 weeks post-surgery. Micro-CT and histological analyses confirmed successful functional recovery and fibrocartilage regeneration at the ligament-bone interface. Gas cavities observed post-implantation resolved by week 12 without anchor dislocation. The rapid degradation of threaded region released magnesium ions to facilitate osteogenesis, while the slower degradation of anchor eyelet maintained structural integrity for stable fixation. The gradual decline in fracture force of eyelet parts remained higher than the initial pull-out force within 12 weeks implantation. Furthermore, progressive integration occurred in the connection of assembled anchor further highlighted its reliable fixation performance. This study offers a framework for further design and research of biodegradable magnesium alloy suture anchors for clinical applications.</div></div><div><h3>Statement of significance</h3><div>Achieving clinical efficacy for biodegradable magnesium alloy anchors requires maintaining long-term mechanical stability post-surgery. Delaying degradation at suture-contact anchor eyelet can prolong service life. In this study, we designed an assembled anchor with external full threads to enhance fixation strength while preventing body fluid infiltration into internal anchor eyelet to retard its degradation. Multi-scale <em>in vitro/vivo</em> studies revealed that rapid degradation of external threads promoted bone-tissue integration, whereas slower-degrading anchor eyelet preserved structural stability. Notably, the fracture strength at 12 weeks post-implantation remained superior to the initial pull-out strength. These findings demonstrate the potential for broadening clinical applications of magnesium alloy anchors in future trials.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"205 ","pages":"Pages 723-736"},"PeriodicalIF":9.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144981962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}