Microbial Biotechnology最新文献

Characterization of the Orphan Cytochrome P450 CYP107J1 From Bacillus subtilis Through Peroxygenase Activity Engineering. 枯草芽孢杆菌孤儿细胞色素P450 CYP107J1的过加氧酶活性工程研究

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-05-01 DOI: 10.1111/1751-7915.70369

Hideki Kato, Takafumi Hashimoto, Tuhin Das, Daniel Z Doherty, Stephen G Bell, Toshiki Furuya

{"title":"Characterization of the Orphan Cytochrome P450 CYP107J1 From Bacillus subtilis Through Peroxygenase Activity Engineering.","authors":"Hideki Kato, Takafumi Hashimoto, Tuhin Das, Daniel Z Doherty, Stephen G Bell, Toshiki Furuya","doi":"10.1111/1751-7915.70369","DOIUrl":"https://doi.org/10.1111/1751-7915.70369","url":null,"abstract":"Cytochrome P450 monooxygenases (P450s) not only play many physiological roles in oxidative metabolism but are also promising biocatalysts for the synthesis of organic molecules. Bacillus subtilis strain 168, which possesses eight P450 genes, has been extensively studied as a model bacterium, but the catalytic function of CYP107J1 remains to be fully elucidated. Here, we investigated the catalytic function of CYP107J1. Because the genes encoding the reductase components of CYP107J1 could not be identified from the genome sequence, putidaredoxin (Pdx) and its reductase (PdR) were first used to reconstitute the enzymatic activity of CYP107J1. The enzyme showed oxidation activity toward 4-alkylbenzoic acids with a carbon chain length of 3-8, although the activity was low. To enhance the activity of CYP107J1, over that observed when using heterologous redox partners, we next engineered CYP107J1 into a hydrogen peroxide-driven form (i.e., peroxygenase). Only two amino acid substitutions in the active site of CYP107J1 were required for a peroxygenase variant exhibiting 28 times higher catalytic activity toward 4-hexylbenzoic acid than the wild-type enzyme supported by Pdx and PdR. This highly active enzyme enabled detailed characterization of CYP107J1. Interestingly, the engineered enzyme not only oxidized 4-alkylbenzoic acids but also efficiently produced the valuable dye indigo by simply mixing the enzyme with indole and hydrogen peroxide. Enzymes of the CYP107J subfamily are widely distributed among bacteria of genus Bacillus. The findings from this study will facilitate further exploitation of the catalytic potential of CYP107J subfamily enzymes.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":"e70369"},"PeriodicalIF":5.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arsenal of Aromatic Degrading Bacteria: How They Sense, Chase, Adapt and Destroy Environmental Pollutants. 芳香降解细菌库：它们如何感知、追逐、适应和破坏环境污染物。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-05-01 DOI: 10.1111/1751-7915.70372

Prashant S Phale, Tushar Dhamale, Satyajit Subhash

{"title":"The Arsenal of Aromatic Degrading Bacteria: How They Sense, Chase, Adapt and Destroy Environmental Pollutants.","authors":"Prashant S Phale, Tushar Dhamale, Satyajit Subhash","doi":"10.1111/1751-7915.70372","DOIUrl":"10.1111/1751-7915.70372","url":null,"abstract":"Aromatic compounds are ubiquitous arising from natural sources as well as anthropogenic activities posing significant ecological and health risks due to their persistence and toxicity in nature. While bacterial biodegradation of these compounds offers a sustainable strategy, its success usually hinges on integrated phenotypes that are beyond mere catabolic pathways. Phenotype involves multiple processes like sensing pollutants, chemotaxis, transport, membrane adaptation, stress tolerance, regulation at molecular level, and community co-operation. Bacteria sense aromatics via specialized chemoreceptors, triggering metabolism-dependent or independent chemotaxis. Partitioning of hydrophobic pollutants into membranes is countered by membrane modifications and efflux pumps. While facilitated uptake occurs using biosurfactants and specific transporters. Some bacteria exhibit unique carbon-source utilization hierarchies that prioritize aromatics over other carbon sources or co-metabolize, subverting canonical catabolite repression leading to niche dominance. Biofilm formation, cross-feeding and division of labor enhance resilience in bacterial communities. Understanding and integrating these sensing, chemotactic, adaptive and metabolic capabilities are crucial for the rational engineering of bacteria for effective remediation of contaminated sites.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":"e70372"},"PeriodicalIF":5.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Followers' Choice: The Trends Transforming Precision Medicine, Synthetic Biology, and Sustainable Microbiology. 追随者的选择：改变精准医学、合成生物学和可持续微生物学的趋势。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-05-01 DOI: 10.1111/1751-7915.70370

Patricia Bernal, Rocío Palacios-Ferrer, Juan L Ramos

引用次数: 0

Genome-Scale Engineering Importing Property of Escherichia coli for Improving Production of 3-Hydroxypropionic Acid. 大肠杆菌基因工程导入特性提高3-羟基丙酸产量。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-05-01 DOI: 10.1111/1751-7915.70362

Seungjin Kim, Junyeong Sung, Ga Yeon Lim, Gyoo Yeol Jung, Hyun Gyu Lim

{"title":"Genome-Scale Engineering Importing Property of Escherichia coli for Improving Production of 3-Hydroxypropionic Acid.","authors":"Seungjin Kim, Junyeong Sung, Ga Yeon Lim, Gyoo Yeol Jung, Hyun Gyu Lim","doi":"10.1111/1751-7915.70362","DOIUrl":"10.1111/1751-7915.70362","url":null,"abstract":"Microbial biochemical production can suffer reduced growth and productivity from intracellular product accumulation, which can be mitigated by minimizing product import. Limited understanding of import-related genes, especially for non-native products, has hindered this approach in strain development. We developed a workflow to identify genes involved in 3-hydroxypropionic acid (3-HP) import. We constructed a genome-wide overexpression library coupled with a 3-HP-responsive fluorescent biosensor and used flow cytometry to isolate narQ-overexpressing strains with a 3.0-fold higher fluorescence signal. Transcriptome profiling under NarQ overexpression revealed a distinct set of membrane-associated genes (acrD, mliC, and pgaABCD) that were transcriptionally upregulated, and functional tests confirmed that their overexpression enhanced 3-HP import while their deletion in producing strains increased 3-HP titers by up to 21% compared with the control strain. This study provides a systematic workflow for identifying import-related genes directly from genomic DNA, advancing the development of more efficient microbial production platforms.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":"e70362"},"PeriodicalIF":5.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13124679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Small Molecule Accelerates Early Persister Regrowth and Potentiates Antibiotic Killing via MdtL-DcrB. 一种新的小分子通过MdtL-DcrB加速早期持久性再生并增强抗生素杀伤。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-05-01 DOI: 10.1111/1751-7915.70368

Garin Park, Hyein Kim, Sooyeon Song

{"title":"A Novel Small Molecule Accelerates Early Persister Regrowth and Potentiates Antibiotic Killing via MdtL-DcrB.","authors":"Garin Park, Hyein Kim, Sooyeon Song","doi":"10.1111/1751-7915.70368","DOIUrl":"10.1111/1751-7915.70368","url":null,"abstract":"Persister cells survive antibiotic exposure through transient tolerance, often leading to infection relapse. Because antibiotic susceptibility is restored when persisters resume growth, we sought to identify a chemical modulator that advances early regrowth and to define the pathway underlying its activity. A screen of 7040 compounds led to the discovery of bymBDZ, which shortens the lag phase and promotes early regrowth in persister-derived Escherichia coli. bymBDZ significantly enhanced antibiotic killing during early treatment windows when survivors are typically retained, and this activity extended to enterohemorrhagic E. coli O157:H7 and multiple antibiotic classes. Genetic and functional analyses showed that bymBDZ activity required the membrane transporter MdtL and the envelope factor DcrB. bymBDZ induced dcrB expression and remodelled envelope-associated transport, resulting in increased intracellular exposure to small molecules during early regrowth, as indicated by elevated dye accumulation. Consistent with this remodelling, bymBDZ promoted faster growth resumption and reinforced antibiotic killing during early regrowth. Collectively, these findings identify bymBDZ as a chemical probe that modulates persister regrowth through MdtL-DcrB-dependent envelope transport remodelling and suggest a strategy to sensitize tolerant bacteria to antibiotics.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":"e70368"},"PeriodicalIF":5.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147808965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine Learning Reveals Quantitative Amino Acid Preferences in Bifidobacterium longum Growth. 机器学习揭示长双歧杆菌生长中的定量氨基酸偏好。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-05-01 DOI: 10.1111/1751-7915.70367

Hiroki Kaneko, Kana Kadowaki, Shin Yoshimoto, Toshitaka Odamaki, Bei-Wen Ying

{"title":"Machine Learning Reveals Quantitative Amino Acid Preferences in Bifidobacterium longum Growth.","authors":"Hiroki Kaneko, Kana Kadowaki, Shin Yoshimoto, Toshitaka Odamaki, Bei-Wen Ying","doi":"10.1111/1751-7915.70367","DOIUrl":"10.1111/1751-7915.70367","url":null,"abstract":"Bifidobacterium longum is a prevalent human gut symbiont whose carbohydrate metabolism is well characterized, whereas the quantitative contribution of amino acids to growth remains unclear. Here, we combined genome-based pathway analysis, growth phenotyping in chemically defined media, and iterative machine-learning-guided medium design to quantify amino acid preferences in B. longum subsp. longum JCM 1217T. Genome analysis predicted cysteine as the sole auxotrophy, and experiments confirmed that cysteine alone supported growth but did not restore the high maximum cell density and short lag time achieved with a complete amino acid mixture. Regression models and genetic algorithms identified amino acid formulations in the selected optimized compositions that reduced total amino acid input by 66.5% under glucose and 77.2% under lactose while maintaining growth comparable to complete medium. SHAP analysis highlighted tyrosine as the main determinant of maximum cell density, whereas glutamate, leucine, and valine consistently shortened lag time. These results show that amino acid requirements in B. longum extend beyond binary auxotrophy and provide a machine-learning framework for designing streamlined defined media.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":"e70367"},"PeriodicalIF":5.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives for Advancing Biotechnological Succinic Acid Production. 推进生物技术琥珀酸生产的展望。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-05-01 DOI: 10.1111/1751-7915.70363

Christoph Gunkel, Bastian Blombach

{"title":"Perspectives for Advancing Biotechnological Succinic Acid Production.","authors":"Christoph Gunkel, Bastian Blombach","doi":"10.1111/1751-7915.70363","DOIUrl":"10.1111/1751-7915.70363","url":null,"abstract":"Succinic acid has been considered an important molecule in the transition of chemical manufacturing from fossil-based to sustainable and future-proof processes. While there has been extensive research on biotechnological succinic acid production from biomass, attempts to roll out bio-succinic acid are impeded by its high price and remaining sustainability issues. Both drawbacks are interconnected and can be traced back to the used feedstocks and a wasteful expenditure of acid and base, among others. In this opinion, we discuss biochemical principles and metabolic pathways of next-generation carbon assimilation and low-pH fermentations to address these drawbacks. For this reason, we chart the potential for producing succinic acid from sustainable next-generation feedstocks based on electron, carbon and ATP balances as well as relevant thermodynamic considerations. Furthermore, we summarize key advances in low-pH succinic acid synthesis using acid-tolerant yeasts and assess the suitability of selected acid tolerance mechanisms for industrial application. Eventually, we aim to inspire researchers to synthesize innovative approaches to realize competitive and sustainable biotechnological succinic acid production.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":"e70363"},"PeriodicalIF":5.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13124660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates. tenbrio molitor衍生的酶系统使细胞内聚羟基烷酸酯的溶剂还原回收成为可能。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-05-01 DOI: 10.1111/1751-7915.70360

Sanja Skaro Bogojevic, Brana Pantelic, Marijana Ponjavic, Tatjana Ilic-Tomic, Dusan Milivojevic, Maciej Guzik, Marcin Rychwalski, Romanos Siaperas, Evangelos Topakas, Mónica Carvalheira, Filomena Freitas, Patrícia Concórdio-Reis, Jasmina Nikodinovic-Runic

{"title":"Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates.","authors":"Sanja Skaro Bogojevic, Brana Pantelic, Marijana Ponjavic, Tatjana Ilic-Tomic, Dusan Milivojevic, Maciej Guzik, Marcin Rychwalski, Romanos Siaperas, Evangelos Topakas, Mónica Carvalheira, Filomena Freitas, Patrícia Concórdio-Reis, Jasmina Nikodinovic-Runic","doi":"10.1111/1751-7915.70360","DOIUrl":"10.1111/1751-7915.70360","url":null,"abstract":"Polyhydroxyalkanoates (PHAs) are intracellular microbial polyesters whose commercial deployment is strongly influenced by downstream processing costs and environmental burden. Here, we demonstrate a nearly solvent-free, mild aqueous strategy for the recovery of PHAs directly from wet bacterial biomass using crude enzyme systems derived from Tenebrio molitor. Under optimized conditions (0.6 wt% crude protein, pH 7.6, 40°C), near-quantitative (≥ 95%) recovery of PHB and PHB/HV and up to 60% recovery of mcl-PHA were achieved without prior biomass drying. Proteomic analysis identified abundant digestive hydrolases, including α-amylase and cathepsin and recombinant validation confirmed their contribution to polymer release, demonstrating that targeted enzyme combinations can substantially enhance mcl-PHA recovery (up to 94%-98%). Gel permeation chromatography, NMR and thermal analysis demonstrated preservation of polymer molecular integrity and crystallinity comparable to chloroform extraction. Life cycle assessment revealed a three- to seven-fold reduction in carbon footprint relative to conventional solvent and classical enzymatic methods, primarily due to elimination of drying and solvent use. These findings establish biologically driven biomass hydrolysis as a scalable downstream strategy and highlight insect-derived enzyme systems as promising tools for integrated microbial biopolymer processing.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":"e70360"},"PeriodicalIF":5.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13130152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled Low-Oxygen Supply Enables Magnetosome Size Tuning by Uncoupling Magnetite Nucleation and Crystal Growth in Magnetospirillum gryphiswaldense 受控低氧供应通过解偶联磁铁矿成核和晶体生长实现磁小体尺寸调节。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-04-14 DOI: 10.1111/1751-7915.70349

Sophia Tessaro, Markus Schüritz, Valérie Jérôme, Ruth Freitag, René Uebe

{"title":"Controlled Low-Oxygen Supply Enables Magnetosome Size Tuning by Uncoupling Magnetite Nucleation and Crystal Growth in Magnetospirillum gryphiswaldense","authors":"Sophia Tessaro, Markus Schüritz, Valérie Jérôme, Ruth Freitag, René Uebe","doi":"10.1111/1751-7915.70349","DOIUrl":"10.1111/1751-7915.70349","url":null,"abstract":"The magnetotactic bacterium Magnetospirillum gryphiswaldense MSR-1 synthesizes membrane-enclosed magnetite (Fe3O4) nanocrystals, known as magnetosomes. Owing to their uniform size, purity and superior magnetic properties, magnetosomes represent highly attractive nanomaterials for biotechnological and biomedical applications. However, their bioproduction is limited by demanding cultivation requirements, largely because magnetite biomineralization is highly sensitive to environmental parameters, particularly oxygen. While elevated oxygen concentrations are known to inhibit magnetosome formation, quantitative analyses under defined low-oxygen conditions are scarce. Here, we cultivated MSR-1 in bioreactors under precisely controlled dissolved oxygen (DO) levels and quantified growth behaviour, substrate uptake and magnetosome characteristics. Cells harvested during late exponential growth revealed that magnetite crystal numbers per cell were similar across a wide DO range (0%–5%), whereas crystal sizes decreased with increasing oxygen levels. The data further indicate that oxygen inhibits biomineralization primarily through direct oxidative interference rather than indirect metabolic effects. These findings provide a mechanistic basis for optimizing oxygen control strategies in MTB cultivation and demonstrate that fine-tuning DO levels enables targeted modulation of magnetosome size and properties. This advances both the bioprocess development of high-yield magnetosome production and the application of tailored magnetic nanoparticles in biotechnology and medicine.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 4","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70349","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overriding Bioprocess Perturbations With a Cell–Machine Interface for Reliable Microbial Stress-Response Control 用一个可靠的微生物应激反应控制的细胞-机器接口压倒生物过程扰动。

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2026-04-13 DOI: 10.1111/1751-7915.70329

Mathéo Delvenne, Juan Andres Martinez, Cees Haringa, Henk Noorman, Steven Minden, Ralf Takors, Frank Delvigne

{"title":"Overriding Bioprocess Perturbations With a Cell–Machine Interface for Reliable Microbial Stress-Response Control","authors":"Mathéo Delvenne, Juan Andres Martinez, Cees Haringa, Henk Noorman, Steven Minden, Ralf Takors, Frank Delvigne","doi":"10.1111/1751-7915.70329","DOIUrl":"10.1111/1751-7915.70329","url":null,"abstract":"Controlling cell population dynamics and phenotypic diversification is a key objective in systems and synthetic biology, particularly for ensuring uniform responses from engineered gene circuits. While cell–machine interfaces have been employed to modulate host–gene circuit interactions, environmental perturbations typical of industrial bioreactor conditions remain underexplored. In this study, we investigate the impact of such perturbations on the general stress response in Escherichia coli and Saccharomyces cerevisiae. Using scale-down bioreactor experiments, we evaluate the performance of the Segregostat, a real-time control system that leverages automated flow cytometry to induce dynamic nutrient shifts. The Segregostat achieves robust stress response control, even under severe perturbations such as extended residence times in a two-compartment reactor. We hypothesise that this robustness arises from the system's ability to amplify host-compatible fluctuations beyond bioreactor-induced perturbations. Our findings highlight the importance of integrating environmental factors into control strategies for reliable gene circuit behaviour in industrial bioprocessing environments.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 4","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70329","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0