Microbial Biotechnology最新文献

{"title":"Harnessing the Ecological and Genomic Adaptability of the Bacterial Genus Massilia for Environmental and Industrial Applications","authors":"Kamyar Amirhosseini,&nbsp;Mehrdad Alizadeh,&nbsp;Hamed Azarbad","doi":"10.1111/1751-7915.70156","DOIUrl":"https://doi.org/10.1111/1751-7915.70156","url":null,"abstract":"<p>The bacterial genus <i>Massilia</i> was first described in 1998, and since then has attracted growing interest due to its ecological plasticity and biotechnological promise. Certain species of the genus <i>Massilia</i> inhabit a variety of ecosystems, from arid deserts to polar glaciers, and exhibit unique adaptations such as resistance to cold and heat. In contaminated environments, some members of <i>Massilia</i> contribute significantly to the detoxification of heavy metals and the degradation of organic pollutants, presenting them as promising agents for bioremediation. In addition, <i>Massilia</i> species improve plant resistance and facilitate pollutant absorption in phytoremediation strategies. New research also highlights their potential as bioindicators of environmental health, given their abundance in anthropogenically influenced ecosystems and airborne microbial communities. In addition to their ecological roles, some <i>Massilia</i> species have potential in biotechnological applications by producing biopolymers and secondary metabolites. Here, we integrate findings across various habitats to present a comprehensive overview of the ecological and biotechnological importance of the genus <i>Massilia</i>. We highlight critical knowledge gaps and propose future research directions to fully harness the potential of this not fully explored bacterial genus to address environmental challenges, including contamination.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70156","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143909022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fimsbactin Siderophores From a South African Marine Sponge Symbiont, Marinomonas sp. PE14-40","authors":"Nompumelelo Philile Praiseworth Ikegwuoha,&nbsp;Thea Hanekom,&nbsp;Elzaan Booysen,&nbsp;Corbyn Jason,&nbsp;Shirley Parker-Nance,&nbsp;Michael T. Davies-Coleman,&nbsp;Leonardo Joaquim van Zyl,&nbsp;Marla Trindade","doi":"10.1111/1751-7915.70155","DOIUrl":"https://doi.org/10.1111/1751-7915.70155","url":null,"abstract":"<p>Low iron levels in marine habitats necessitate the production of structurally diverse siderophores by many marine bacterial species for iron acquisition. Siderophores exhibit bioactivities ranging from chelation for iron reduction in hemochromatosis sufferers to antimicrobial activity either in their own right or when coupled to known antibiotics for targeted delivery or for molecular imaging. Thus, marine environments are a sought-after resource for novel siderophores that could have pharmaceutical or industrial application. The fimsbactins A-F (<b>1–6</b>) are mixed catechol-hydroxamate siderophores that have only been reported to be produced by <i>Acinetobacter</i> species with the fimsbactin biosynthetic gene clusters (BGCs) widespread among species within this genus. Here, we identified a putative fimsbactin BGC from an uncharacterized marine isolate, <i>Marinomonas</i> sp. PE14-40. Not only was the gene synteny not conserved when comparing the pathway from <i>Marinomonas</i> sp. PE14-40 to the fimsbactin BGC from <i>Acinetobacter</i> sp., but five of the core biosynthetic genes found in the canonical fimsbactin BGC are located elsewhere on the genome and do not form part of the core cluster in <i>Marinomonas</i> sp. PE14-40, with four of these, <i>fbsBCDL,</i> colocalized. Through ESI-MS/MS analysis of extracts from <i>Marinomonas</i> sp. PE14-40, the known fimsbactin analogues <b>1</b> and <b>6</b> were identified, as well as two new fimsbactin analogues, <b>7</b> and <b>8</b>, containing a previously unreported L-lysine-derived hydroxamate moiety, <i>N</i>_<i>1</i>-acetyl-<i>N</i>_<i>1</i>-hydroxycadaverine. Feeding experiments using stable isotope-label L-lysine provided further evidence of the <i>N</i>_<i>1</i>-acetyl-<i>N</i><i>₁</i>-hydroxycadaverine moiety in <b>7</b> and <b>8</b>. The study demonstrates functional conservation in seemingly disparate biosynthetic pathways and enzyme promiscuity's role in producing structurally diverse compounds.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review","authors":"Yasaman Ahmadi,&nbsp;Yejiong Yu,&nbsp;Zhanfeng Cui,&nbsp;Wei E. Huang,&nbsp;Monique I. Andersson","doi":"10.1111/1751-7915.70153","DOIUrl":"https://doi.org/10.1111/1751-7915.70153","url":null,"abstract":"<p>Sexually transmitted infections (STIs) remain a significant public health concern. Given the asymptomatic nature of many STIs, diagnostic testing is critical for determining the appropriate treatment, enabling effective tracing and reducing the risk of further transmission. Nucleic acid amplification tests (NAATs) are the most sensitive and the most widely used in well-resourced settings. The majority of available NAATs are based on polymerase chain reaction (PCR), which requires highly trained personnel and costly equipment, making it impractical for resource-limited settings. Loop-mediated isothermal amplification (LAMP) has emerged as a simple, rapid, sensitive and low-cost alternative for pathogen detection, particularly well-suited for point-of-care tests (POCT). In this review, we evaluate LAMP assays reported in the literature for the detection of pathogens linked to the high incidence STIs prioritised by the World Health Organization (WHO) for POCT in 2023. These include <i>Neisseria gonorrhoeae</i>, <i>Chlamydia trachomatis</i>, <i>Trichomonas vaginalis</i>, <i>T. pallidum</i> subspecies <i>pallidum</i>, as well as other common STIs such as herpes simplex virus, hepatitis B virus and human immunodeficiency virus (HIV). For each LAMP assay, we identified and summarised the key elements such as the type and number of tested clinical specimens, chosen target gene, detection system, reference test and clinical outcomes. We highlight the advantages and limitations of these assays and discuss the gaps that should be addressed to improve their applicability for POCT.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Respiratory Tract Microbiome and Human Health","authors":"Patricia Fernández de Córdoba-Ansón,&nbsp;Iván Linares-Ambohades,&nbsp;Fernando Baquero,&nbsp;Teresa M. Coque,&nbsp;Ana Elena Pérez-Cobas","doi":"10.1111/1751-7915.70147","DOIUrl":"https://doi.org/10.1111/1751-7915.70147","url":null,"abstract":"<p>The respiratory tract microbiome (RTM) is a multi-kingdom microbial ecosystem that inhabits various niches of the respiratory system. While previously overlooked, there is now sufficient evidence that the RTM plays a crucial role in human health related to immune system training and protection against pathogens. Accordingly, dysbiosis or disequilibrium of the RTM has been linked to several communicable and non-communicable respiratory diseases, highlighting the need to unveil its role in health and disease. Here, we define the RTM and its place in microbiome medicine. Moreover, we outline the challenges of RTM research, emphasising the need for combining methodologies, including multi-omics and computational tools. We also discuss the RTM's potential for diagnosing, preventing and treating respiratory diseases and developing novel microbiome-based therapies to improve pulmonary health.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143880148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Alginate Lyase Engineering for Efficient Conversion of Alginate to Value-Added Products","authors":"Hyo Jeong Shin,&nbsp;Jo Hyun Moon,&nbsp;Sunghwa Woo,&nbsp;Chung Won Lee,&nbsp;Gyoo Yeol Jung,&nbsp;Hyun Gyu Lim","doi":"10.1111/1751-7915.70150","DOIUrl":"https://doi.org/10.1111/1751-7915.70150","url":null,"abstract":"<p>Alginate lyases depolymerize alginate and generate alginate oligosaccharides (AOS) and eventually 4-deoxy-L-erythro-5-hexoseulose uronate (DEH), a monosaccharide. Recently, alginate lyases have garnered significant attention due to the increasing demand for AOS, which exhibit bioactivities beneficial to human health, livestock productivity, and agricultural efficiency. Additionally, these enzymes play a crucial role in producing DEH, essential in alginate catabolism in bacteria. This review explains the industrial value of AOS and DEH, which contribute broadly to industries ranging from the food industry to biorefinery processes. This review also highlights recent advances in alginate lyase applications and engineering, including domain truncation, chimeric enzyme design, rational mutagenesis, and directed evolution. These approaches have enhanced enzyme performance for efficient AOS and DEH production. We also discuss current challenges and future directions toward industrial-scale bioconversion of alginate-rich biomass.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143880107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Greening the Production of Indigo Blue Exploiting Light and a Recombinant Synechocystis sp. PCC6803 Strain Expressing the Enzyme mFMO","authors":"Giovanni Loprete,&nbsp;David Rubert,&nbsp;Francesco Bellusci,&nbsp;Nikola Lončar,&nbsp;Marco W. Fraaije,&nbsp;Elisabetta Bergantino","doi":"10.1111/1751-7915.70146","DOIUrl":"https://doi.org/10.1111/1751-7915.70146","url":null,"abstract":"<p>Cyanobacteria are emerging as interesting cell factories, offering the significant advantage of their in-built photosynthetic machinery, which generates NADPH to support redox biocatalysis. In this study, we assessed the potential of the cyanobacterium <i>Synechocystis</i> sp. PCC6803 in producing the dye indigo by light-driven whole-cell biotransformation using indole as a starting compound. A stable transgenic strain expressing a flavin-containing monooxygenase from <i>Methylophaga aminisulfidivorans</i> (mFMO) was engineered, enabling light-dependent indigo production. Upon optimising conditions, effective biotransformations could be performed, resulting in 112 mg/L indigo (86% conversion of the furnished indole). Additionally, we present a method for the recovery of the secreted dye directly from the growth medium through solid-phase absorption on polyamide nets. Overall, the effectiveness and sustainability of the biotransformation in <i>Synechocystis</i> sp. PCC6803 performed at the laboratory scale provide a strong basis for further exploring the applicability of the process.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143880109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of CRISPR-STAR System to Realise Simultaneous Transcriptional Activation and Repression in Yarrowia lipolytica","authors":"Yaru Chen,&nbsp;Mengxu Li,&nbsp;Xuanwei Liu,&nbsp;Qiyang Duan,&nbsp;Lin Xiao,&nbsp;Luxin Wang,&nbsp;Congcong Huang,&nbsp;Hao Song,&nbsp;Yingxiu Cao","doi":"10.1111/1751-7915.70151","DOIUrl":"https://doi.org/10.1111/1751-7915.70151","url":null,"abstract":"<p>The ability to regulate gene expression in multiple directions is crucial to maximise the production of microbial cell factories. However, the lack of a regulatory tool that can simultaneously activate and repress multiple genes restricts the manipulation diversity of <i>Yarrowia lipolytica</i>, which is an industrial workhorse for bioproduction. To address this issue, we developed a CRISPR <span>s</span>caffold RNAs (scRNAs)-mediated <span>t</span>ranscriptional <span>a</span>ctivation and <span>r</span>epression (CRISPR-STAR) platform. Firstly, we evaluated different methods for bidirectional regulation using CRISPR on both endogenous and synthetic promoters in <i>Y. lipolytica</i>, and chose the utilisation of orthogonal scRNAs to recruit activation and inhibition domains. Secondly, CRISPR-STAR was optimised by the introduction of alternative dCas proteins, scRNA structures and activators. 2.6-fold and 54.9-fold activation were achieved for synthetic and endogenous promoters, respectively, when the VPR transcriptional activator was recruited via MS2 hairpin. The repression of several genes was successfully achieved, with repression levels ranging from 3% to 32%, when the MXI1 transcriptional repressor was recruited via PP7 hairpin. Finally, CRISPR-STAR was applied to enhance fatty alcohol production by activating the <i>FAR</i> gene (encodes fatty acyl-CoA reductase) and repression of the <i>PEX10</i> gene (encodes an integral membrane protein required for peroxisome biogenesis and matrix protein import). Compared to the non-targeting control, the bidirectionally regulated strain showed a 55.7% increase in yield to 778.8 mg/L. Our findings demonstrate that the CRISPR-STAR platform enables multi-mode regulation of genes, offering engineering opportunities to improve the productive performance of <i>Y</i>. <i>lipolytica</i>.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Glucose to Green Chemistry: Breakthrough in Microbial Production of Tartaric Semialdehyde","authors":"Shuangxi Li,&nbsp;Lingcheng Li,&nbsp;Qiwu Jiang,&nbsp;Jianfeng Wang,&nbsp;Xiaoming Sun,&nbsp;Liangliang Zhang,&nbsp;Jianfeng Yuan","doi":"10.1111/1751-7915.70149","DOIUrl":"https://doi.org/10.1111/1751-7915.70149","url":null,"abstract":"<p>L-(+)-tartaric acid (L-TA) is a crucial hydroxy carboxylic chelator with extensive applications in the food and pharmaceutical industries. The synthesis of L-TA from renewable biomass presents a promising approach to mitigating environmental impact and advancing green energy initiatives. Previous studies revealed that a mutant transketolase (TKTA_M) could catalyse the production of tartaric semialdehyde, a precursor to L-TA. This study focuses on the development of a <i>Gluconobacter oxydans</i> cell factory for tartaric semialdehyde production, employing a combination of metabolic engineering and a modular strategy. The genetically modified <i>G. oxydans</i> T strain exhibited robust expression of the <i>tkt</i>A_M gene. The optimal pH and temperature for this strain were determined to be 6.0°C and 30°C, respectively. Under these conditions, the strain produced 32.21 ± 0.74 g/L of tartaric semialdehyde from glucose. Implementation of a “Push-Pull” strategy enhanced tartaric semialdehyde production, resulting in a 23.85% increase in the <i>G. oxydans</i> T02 cell growth. In CSLP medium with 100 g/L glucose, the fermentation process yielded 48.88 ± 2.16 g/L of tartaric semialdehyde and 7.72 ± 1.56 g/L of residual 5-KGA after 48 h. This resulted in a tartaric semialdehyde productivity rate of 1.018 g/L·h, representing an 87.82% improvement over flask fermentation. This study demonstrates a straightforward and efficient microbial process for the oxidation of glucose to tartaric semialdehyde, indicating its potential for industrial-scale production and facilitating the synthesis of L-TA from renewable resources.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Sugar Metabolism During Fermentation of Brewers' Spent Grain by Leuconostoc pseudomesenteroides DSM20193","authors":"Koirala Prabin,&nbsp;Maina Ndegwa,&nbsp;Mojzita Dominik,&nbsp;Coda Rossana","doi":"10.1111/1751-7915.70116","DOIUrl":"https://doi.org/10.1111/1751-7915.70116","url":null,"abstract":"<p>Re-utilising brewers' spent grain (BSG) through LAB fermentation can enable its broad use in the food industry, enhancing its nutritional and functional properties and offering a clear example of a sustainable approach in the valorisation of food side streams. Despite extensive research on LAB fermentation, the regulation of metabolism during the growth in complex food-industry-relevant environments remains unclear. This study investigates the metabolic processes in <i>Leuconostoc pseudomesenteroides</i> DSM20193 during 24 h fermentation of BSG with and without 4% sucrose (w/w) supplementation, allowing in situ dextran synthesis. Besides dextran synthesis, the presence of sucrose led to faster acidification, especially due to the increased formation of acetic acid. Furthermore, differences in the utilisation of sucrose, fructose, glucose, and maltose and the formation of diverse oligosaccharides were observed. Transcriptome analysis comparing expression profiles during 0 h and 16 h growth in BSG with sucrose revealed differences in the expression of genes involved in carbohydrate utilisation pathways, including higher activity of sucrose and maltose metabolism and lower activity of metabolism related to alternative carbon sources. Transcription analysis of selected relevant genes in a time-course comparison between BSG with and without sucrose provided more detailed indications of responses of the metabolic network in this complex environment. This analysis provided a deeper understanding of the dynamic regulatory mechanism that drives sugar metabolism and dextran synthesis and how the presence of sucrose can alter the metabolic flux towards different fermentation products.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Isotopic Tracer and Modelling Approach Reveals Differences in Nitrogen Metabolism in S. cerevisiae, S. uvarum and S. kudriavzevii Species","authors":"Romain Minebois,&nbsp;David Henriques,&nbsp;Eva Balsa-Canto,&nbsp;Amparo Querol,&nbsp;Carole Camarasa","doi":"10.1111/1751-7915.70087","DOIUrl":"https://doi.org/10.1111/1751-7915.70087","url":null,"abstract":"<p>The species <i>Saccharomyces uvarum</i> and <i>Saccharomyces kudriavzevii</i> have gained popularity in recent decades due to their interesting oenological properties. However, although it plays a crucial role in yeast fermentation performance and compound synthesis, our understanding of nitrogen metabolism in these species remains limited. Therefore, we compared how three strains of <i>Saccharomyces cerevisiae</i>, <i>Saccharomyces uvarum</i> and <i>Saccharomyces kudriavzevii</i> use relevant nitrogen sources by combining quantitative analysis approaches based on isotopic tracing and modelling. The model we have developed aims to facilitate the calculation and interpretation of stable isotope data for other experiments, by providing easy visualisation of the results and predicting the kinetics of isotope incorporation beyond the sampling points. The three species exhibit significant variations in their nitrogen assimilation profile. They differ in the timing of uptake of ammonium, arginine and glutamine: <i>Saccharomyces cerevisiae</i> prefers glutamine, <i>Saccharomyces kudriavzevii</i> ammonium and <i>Saccharomyces uvarum</i> arginine. This contributes to a different pattern of nitrogen redistribution towards proteinogenic amino acids between strains at the start of the exponential phase, which fades on entering the stationary phase. Additionally, we found that the contribution of leucine and valine to isoamyl alcohol production varies between species; also, <i>Saccharomyces kudriavzevii</i> activates the synthesis of volatile compounds earlier.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}