Microbial Biotechnology最新文献

{"title":"Comparative Genomic Assessment of the Cupriavidus necator Species for One-Carbon Based Biomanufacturing","authors":"Magnus G. Jespersen,&nbsp;Emil Funk Vangsgaard,&nbsp;Mariana Arango Saavedra,&nbsp;Stefano Donati,&nbsp;Lars K. Nielsen","doi":"10.1111/1751-7915.70201","DOIUrl":"https://doi.org/10.1111/1751-7915.70201","url":null,"abstract":"<p>The transition from a petroleum-based manufacturing to biomanufacturing is an important step towards a sustainable bio-economy. In particular, biotechnological processes which use one carbon (C1) compounds as feedstock represent an interesting avenue. Many bacterial species evolved naturally to thrive on such compounds, among them <i>Cupriavidus necator,</i> which has been studied in the past due to its range of metabolic capabilities in utilisation and production of compounds of interest. <i>Cupriavidus necator</i> strain H16 is the reference laboratory strain for this species and by far the most extensively studied. In contrast, research efforts and genomic characterisation of other strains within this species have been limited and sporadic. Therefore, the genomic diversity and full metabolic potential across the broader species remain poorly understood. In this work, we collected publicly available genomes along with newly sequenced ones. From a collection of 44 genomes, we curated a final collection of 22 genomes deemed to be <i>C. necator</i>. We examined hallmark metabolic functions, including carbon dioxide fixation, formate assimilation and hydrogen utilisation. We identified methylation motifs and restriction modification systems. Finally, strains ATCC 25207, TA06, and 1978 are proposed as candidate strains of interest based on their genomic make-up and observations from literature. This work provides a comprehensive genomic resource for the <i>C. necator</i> species, facilitating its development as a biomanufacturing platform and advancing our understanding of its metabolic diversity and potential applications.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lyophilised Reverse Transcriptase and Polymerase for Point-of-Care Diagnostics of SARS-CoV-2","authors":"Natalie Mutter,&nbsp;Barbara Posch,&nbsp;Yasmin Gillitschka,&nbsp;Nadezhda Kataeva,&nbsp;Birgit Michalek,&nbsp;Daniel Lager,&nbsp;Filip Staniszewski,&nbsp;Alexandra Schilder,&nbsp;Lidiya Osinkina,&nbsp;Maximilian Westenthanner,&nbsp;Joachim Stehr,&nbsp;Federico Buersgens,&nbsp;Johannes R. Peham","doi":"10.1111/1751-7915.70200","DOIUrl":"https://doi.org/10.1111/1751-7915.70200","url":null,"abstract":"<p>Early diagnosis of pathogens is key to reducing their spreading and to preventing severe health risks. The COVID-19 pandemic showed us the need for rapid point-of-care tests. Here, we describe the preparation of an amplification master mix for point-of-care diagnostics. Therefore, two off-patent amplification enzymes were designed, expressed and purified. The preparation of the key components enables independence from delivery issues, manufacturer portfolio and product information's. For long-term storage and cold-free transport, our fabricated amplification mix was lyophilised. Finally, we applied our lyophilised master mix on an integrated point-of-care diagnostic system and could detect 10 copies/μL COVID-19 RNA. The combination of stable, cold-free reagents with the mobile and low-cost device will allow molecular diagnostics of pathogens in a field or home setting.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70200","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergy of ATP and Meropenem in Stimulating the TCA Cycle to Enhance Killing of Carbapenem-Resistant Acinetobacter baumannii","authors":"Xia Li,&nbsp;Dingyun Feng,&nbsp;Jianxia Zhou,&nbsp;Wenbin Wu,&nbsp;Chunyan He,&nbsp;Wenlei Gan,&nbsp;Wenzheng Zheng,&nbsp;Bo Peng,&nbsp;Tiantuo Zhang","doi":"10.1111/1751-7915.70199","DOIUrl":"https://doi.org/10.1111/1751-7915.70199","url":null,"abstract":"<p>The global prevalence of carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) represents a substantial concern for public health. Exogenous adenosine triphosphate (ATP) affects the bactericidal efficacy of meropenem against CRAB; however, the precise mechanism remains elusive. Here, reprogramming metabolomics was employed to delve into the mechanism underlying ATP-potentiated meropenem lethality against CRAB. Our findings reveal that ATP reprogramming activates the tricarboxylic acid (TCA) cycle in <i>A. baumannii</i>. Notably, the TCA cycle inhibitor malonate antagonised the synergistic bactericidal effect of ATP and meropenem. Activation of the TCA cycle stimulated riboflavin metabolism pathway and the electron transport chain, leading to increased reactive oxygen species (ROS) production. Hydrogen peroxide (H₂O₂) enhanced meropenem-mediated killing of CRAB, while the ROS scavenger α-tocopherol diminished the ATP-potentiated bactericidal effect. Additionally, ATP upregulated the gene expression of outer membrane porins, including <i>omp33-36</i>, <i>oprD</i>, <i>ompW</i>, and <i>ompA</i>, thereby improving membrane permeability and elevating intracellular levels of meropenem. The therapeutic synergy of ATP with meropenem was validated in a mouse model of acute pneumonia. This study not only highlights the potential of ATP as a co-treatment with meropenem but also elucidates the mechanisms by which ATP reverses CRAB resistance, specifically through the promotion of ROS production and enhanced membrane permeability.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70199","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbes as Teachers: Rethinking Knowledge in the Anthropocene","authors":"Rachel Armstrong","doi":"10.1111/1751-7915.70195","DOIUrl":"https://doi.org/10.1111/1751-7915.70195","url":null,"abstract":"<p>This opinion piece proposes that the environmental crises of our time arise from a failure to recognise the vital role of microbes in sustaining life on Earth, where ecosystems have been shaped for billions of years by microbial processes, including oxygen production, nutrient cycling and climate regulation. Yet the idea that microbes can ‘teach’ us how to navigate complexity, adapt across scales, and sustain planetary systems is still marginalised in science, policy, and education. A paradigm shift is proposed: microbes must be reframed as active collaborators in solving global challenges. This perspective is grounded in microbial ecology, Indigenous knowledge, and ethical philosophy, advocating for ‘learning’ through and with microbial life. To institutionalise this transition, policy and educational reforms are urged, centring microbial literacy as a foundation for ecological understanding. By integrating microbial agency into human knowledge systems, societal actions could be realigned with the biochemical and evolutionary logics that have sustained life for millennia. Ultimately, a deeper engagement with microbial knowledge is called for—one that informs a more sustainable future.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Biotechnology as a Catalyst for a Better and More Sustainable World","authors":"Carmen Michán,&nbsp;Juan L. Ramos","doi":"10.1111/1751-7915.70196","DOIUrl":"https://doi.org/10.1111/1751-7915.70196","url":null,"abstract":"<p>Education is our most powerful tool for transforming society and improving human well-being. <i>Microbial Biotechnology</i> has previously addressed how we can contribute to enhance public understanding of microbiology in general, and microbial biotechnology in particular, along with their potential benefits. Here, we summarise several contributions in the Special issue ‘Societally-relevant microbiology and outreach’.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Endosymbiosis of Pedobacter sp. DDGJ Boosts the Growth Potential, Stress Resistance and Productivity of Morchella Mushrooms","authors":"Wenchang Zhang,&nbsp;Mantang Cao,&nbsp;Qi Yin,&nbsp;Jin Zhang,&nbsp;Chenhui Wang,&nbsp;Li Han,&nbsp;Yingli Cai,&nbsp;Xiaofei Shi,&nbsp;Linjing Ma,&nbsp;Yousif Abdelrahman Yousif Abdellah,&nbsp;Fuqiang Yu,&nbsp;Peixin He,&nbsp;Wei Liu","doi":"10.1111/1751-7915.70197","DOIUrl":"https://doi.org/10.1111/1751-7915.70197","url":null,"abstract":"<p>True morels are precious edible and medicinal mushrooms. The sustainable development of morel cultivation necessitates urgent breeding programmes to develop improved varieties or strains. Some soil bacteria, including <i>Pedobacter</i> spp., are recognised as morel's beneficial microbes. In this paper, the potential beneficial bacterium <i>Pedobacter</i> sp. DDGJ with minor chitinolytic activity was isolated and confirmed for improving morel's mycelial growth and identified using a combination of morphological characteristics, 16 S rRNA gene sequencing, and comparative genomics. Then, it was introduced into the hyphal cells of three cultivated <i>Morchella</i> strains (<i>M. sextelata</i> 13, <i>M. eximia</i> SM, and <i>M. importuna</i> Y2) through a confrontation culture method. The successful establishment of artificial endosymbiosis was confirmed by PCR assay, green fluorescent protein gene (<i>gfp</i>) localisation, and the cryo-preparation system conjunction with high-resolution field emission scanning electron microscopy. Laboratory assay indicated that artificial endosymbiosis of the bacterium significantly enhanced morel's growth potential, resistance to allelochemicals of 4-coumaric acid and antagonistic capability against the pathogenic fungus <i>Fusarium oxysporum</i> and soil-dominant fungus <i>Chaetomium globosum</i>. Moreover, the outdoor planting experiment showed significant increase in productivity among the endosymbiotic morel strains. The study provides an additional tool to improve <i>Morchella</i> strains with high yield and strong stress resistance.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phage-Antibiotic Combinations for Pseudomonas: Successes in the Clinic and In Vitro Tenuously Connected","authors":"Rabia Fatima,&nbsp;Alexander P. Hynes","doi":"10.1111/1751-7915.70193","DOIUrl":"https://doi.org/10.1111/1751-7915.70193","url":null,"abstract":"<p>Antimicrobial resistance challenges researchers to innovate strategies to enhance the effectiveness of our existing antibiotics. Bacteriophage (phage, bacterial virus)-antibiotic combinations present a promising synergistic approach, particularly for drug-resistant infections such as those caused by Pseudomonas aeruginosa. This approach offers many advantages: enhanced bacterial killing (both planktonic and biofilm), eliminating persister cells, re-sensitization to drugs, and inhibiting resistance spread by targeting plasmids encoding resistant genes. Interestingly, even phages traditionally excluded from therapy – those capable of entering dormancy in the bacterial host – exhibit unique, potent synergy with antibiotics. Despite these clear in vitro benefits and the comparatively strong performance of phage antibiotic combinations in the clinic, translating in vitro efficacy to patient outcomes remain challenging. The lack of standardized metrics for measuring phage-antibiotic interaction complicates cross-study comparisons. In many instances, it is also difficult to translate these in vitro findings to clinically relevant metrics – for example, increased progeny size in vitro is unlikely to contribute meaningfully to treatment success. Addressing these gaps will allow us to fully harness the potential of phage-antibiotic combinations and bridge the disconnect between in vitro results and clinical success.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Metallo-β-Lactamase AMM-1 From Alteromonas mangrovi Reveals a Cryptic Environmental Reservoir of Carbapenem Resistance","authors":"Xuan Wu,&nbsp;Xinjing Han,&nbsp;Lu Zhu,&nbsp;Ningning Pi,&nbsp;Yi Li,&nbsp;Rong Xiang","doi":"10.1111/1751-7915.70191","DOIUrl":"https://doi.org/10.1111/1751-7915.70191","url":null,"abstract":"<p>Carbapenem resistance driven by metallo-β-lactamases (MBLs) poses a formidable global challenge as these enzymes can degrade a wide range of β-lactam antibiotics, including last-line carbapenems. Despite extensive documentation of MBL-producing pathogens, their evolutionary origins and ecological reservoirs are still poorly understood. Here, we report the discovery and in-depth characterisation of AMM-1, a previously unrecognised B1.2 MBL identified within a metagenome-assembled genome of <i>Alteromonas mangrovi</i> obtained from the Yangtze River Estuary. Comparative sequence analyses and phylogenetics reveal that AMM-1 clusters closely with clinically significant MBLs, underscoring its potential impact to human health. Structural modelling confirms the presence of a conserved di-zinc binding site critical for β-lactam hydrolysis, while heterologous expression in <i>Escherichia coli</i> (<i>E. coli</i>) demonstrates a marked increase in resistance against multiple β-lactam classes, including carbapenems. Phylogenetic depth analysis and ancestral reconstruction delineate AMM-1's distinct evolutionary path, placing it deeper than IMP-1 and SPM-1 but shallower than NDM-1. Flexibility simulations reveal unique active-site loop dynamics (L3 and L10), with reduced mobility in key regions that shape substrate binding stability and spectrum. Notably, AMM-1 is stably located on the host chromosome without flanking mobile genetic elements, suggesting that it may have persisted as a vertically inherited trait rather than a recently acquired component of a mobile resistome. These findings highlight the capacity of environmental microbes to serve as long-standing, cryptic reservoirs of potent resistance determinants, emphasising the need for integrated environmental surveillance and preemptive stewardship strategies. By unveiling the molecular and functional properties of AMM-1, this work provides critical insights into how resistance elements can reside, evolve and potentially mobilise within natural habitats, ultimately informing efforts to predict and mitigate the future emergence of carbapenem-resistant bacterial pathogens.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70191","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How Can ‘Health in All Policies’ Help Maximise the Potential of Microbial Biotechnologies for Health, Equity and Sustainability?","authors":"Margaret J. Douglas,&nbsp;Liz Green,&nbsp;James Timmis,&nbsp;Timo Clemens,&nbsp;Kenneth Timmis","doi":"10.1111/1751-7915.70194","DOIUrl":"https://doi.org/10.1111/1751-7915.70194","url":null,"abstract":"<p>Microbial biotechnologies could affect health through multiple pathways, including impacts on food, nutrition, and the physical, economic, and social environment. Health in All Policies is an approach to ensure that plans and policies in all sectors maximise health gains and minimise any health risks. This approach often uses health impact assessment as a structured process to identify and assess positive and negative health impacts and make recommendations to improve these. There are very few examples where HIA has been applied to the implementation of microbial biotechnologies. As more biotechnologies are developed and implemented, more routine use of HIA could help to avoid adverse effects and realise their potential to improve health and reduce health inequalities. This will require greater awareness and understanding of the breadth of links to health, research to build the evidence base for these links, and governance mechanisms to oversee the development and implementation of microbial biotechnologies that prioritise health, equity and sustainability.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70194","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Bat to Worse: The Pivotal Role of Bats for Viral Zoonosis","authors":"Harald Brüssow","doi":"10.1111/1751-7915.70190","DOIUrl":"https://doi.org/10.1111/1751-7915.70190","url":null,"abstract":"<p>Zoonotic infections are increasingly observed and bats (Chiroptera) are playing a pivotal role here. The causal chain of events has been elucidated for Henipavirus (family: paramyxoviruses) infections. Deforestation combined with climate change has reduced the food sources of Pteropus fruit bats and attracted them to fruit trees planted around piggeries in Malaysia, transmitting Nipah virus to pigs as amplifying hosts and then to pig farmers and abattoir workers. Similar scenarios were seen in Australia where Pteropus bats transmitted Hendra virus to horses as intermediate hosts for human infections. Pteropus bats contaminated palm sap collected in Bangladesh with Nipah virus where fatal human-to-human transmissions occurred annually. Less direct evidence links coronaviruses carried by Rhinolophus bats with SARS and COVID-19 pandemics and a piglet epidemic in China. Rousettus bats living in caves transmitted the Marburg virus (family: filovirus) to miners in Africa. Most cases of human rabies in North America were caused by bat lyssaviruses (family: Rhabdoviruses). Bats are viral reservoir species for various virus families (reovirus, Hepacivirus of Flavivirus family, influenza A viruses). Bats are the only flying mammals which opened enormous evolutionary possibilities resulting in a worldwide radiation with 1400 species. Some bat species are represented by huge populations that come together in extremely crowded resting places that are conducive to viral transmission. Bats have evolved mechanisms that tolerate virus replication but suppress the associated pathology, making them healthy carriers for many viruses. It is speculated that with that strategy bats avoid an arms race with viruses for resistance and anti-resistance mechanisms. The excretion of viruses that are highly pathogenic for other mammalian orders could be used as biological weapons to defend their habitat against intrusion by mammalian competitors, including humans. This hypothesis might explain the increasing involvement of bat viruses in emerging infectious diseases observed in recent decades and expected in the future.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}