Microbial Biotechnology最新文献

{"title":"Opinion on Biofilms as Production Systems","authors":"Carmen Mandel,&nbsp;Miriam Edel,&nbsp;Johannes Gescher","doi":"10.1111/1751-7915.70182","DOIUrl":"10.1111/1751-7915.70182","url":null,"abstract":"<p>Biofilm-based production systems offer enhanced robustness, higher biomass densities and improved genetic stability compared to traditional stirred tank reactors, presenting promising alternatives for sustainable, efficient biotechnological manufacturing despite challenges in reactor design and process control.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced Genetic Heterogeneity for Stable Bioproduction by Harnessing the Bias and Mechanism of Mutation","authors":"Yanting Cao,&nbsp;Yaokang Wu,&nbsp;Xueqin Lv,&nbsp;Jianghua Li,&nbsp;Long Liu,&nbsp;Guocheng Du,&nbsp;Jian Chen,&nbsp;Yanfeng Liu","doi":"10.1111/1751-7915.70162","DOIUrl":"10.1111/1751-7915.70162","url":null,"abstract":"<p>Microbial bioproduction is an important approach to realising green biomanufacturing. However, poor bioproduction stability caused by genetic heterogeneity is one of the important factors limiting its industrial-scale applications. Here, two methods have been developed to reduce genetic heterogeneity in <i>Bacillus subtilis</i>. SiteMuB (the site-dependent mutation bias) was proposed to enable stable genome integration expression by analysing the spontaneous mutation rate of the same DNA sequences integrated at different genome sites. Additionally, robustly growing chassis with low mutation rates (ChassisLMR) were developed by deleting unstable elements and enhancing DNA repair. These methods were then employed to improve the production stability of small molecule metabolites and proteins. In <i>N</i>-acetylneuraminic acid production, after 76 generations of cell division, corresponding to the number of cell generations required for &gt; 200-m³ industrial-scale production, strains with SiteMuB and ChassisLMR achieved 15.9-fold and 11.1-fold higher titres than that of the starting strain, respectively. Moreover, by improving the genetic stability of burdensome T7RNAP, combining SiteMuB with ChassisLMR stably maintained the T7 expression system for up to 74 generations, representing a 2.1-fold improvement. Furthermore, ChassisLMR improved the production stability of GFP on the plasmids by 1.38-fold. Overall, SiteMuB and ChassisLMR provide broadly applicable and highly efficient ways to achieve stable bioproduction by reducing genetic heterogeneity.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the Mechanism of Temporal Adaptation to Hydrogen Peroxide-Induced Oxidative Stress in Corynebacterium glutamicum","authors":"Chang Yu,&nbsp;Wenjing Hu,&nbsp;Xiaoyu Li,&nbsp;Yu Lei,&nbsp;Dandan Gao,&nbsp;Meng Wang,&nbsp;Ping Zheng,&nbsp;Yan Zhu,&nbsp;Jibin Sun","doi":"10.1111/1751-7915.70170","DOIUrl":"10.1111/1751-7915.70170","url":null,"abstract":"<p><i>Corynebacterium glutamicum</i> serves as a pivotal industrial chassis for biomanufacturing and an ideal model for studying the phylogenetically related pathogen <i>Mycobacterium tuberculosis</i>. Oxidative stress poses a critical challenge to microorganisms during aerobic industrial processes and immune cell-mediated antibacterial killing by perturbing cellular redox homeostasis, affecting central metabolism, and damaging the integrity of biomacromolecules. However, the intricate mechanisms underlying the dynamic defence of <i>C. glutamicum</i>, despite previous transcriptomic studies on acute and adaptive responses to oxidative stresses, remain largely unclear, hindering strain engineering for industrial applications and the development of effective antimicrobial treatments. In this study, the susceptibility of <i>C. glutamicum</i> to hydrogen peroxide (H₂O₂) was evaluated, and the inhibitory dynamics of H₂O₂ were characterised through viable cell counting. RNA sequencing (RNA-seq) was employed to analyse gene expression changes after exposure to 720 mM H₂O₂. The treatment induced differential expression of 966 and 787 genes at 2 and 6 h, respectively, reflecting perturbations across a broad array of pathways, including (i) enhanced H₂O₂ and peroxide scavenging, mycothiol biosynthesis, and iron chelation; (ii) repressed central metabolism and enhanced anaplerosis; (iii) elevated sulphur assimilation; (iv) altered amino acid biosynthesis; and (v) altered transcriptional regulation in response to oxidative stress. Further validation by overexpression of <i>ahpD</i>, <i>cysN</i>, and exogenous supplementation with <span>l</span>-methionine and <span>l</span>-cysteine significantly enhanced bacterial tolerance to H₂O₂. Overall, this study provides the most comprehensive analysis to date of temporal cellular adaptation to H₂O₂ stress in <i>C. glutamicum</i>, establishing a foundation for future applications in both biomanufacturing and antimicrobial research.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Journals Operating Predatory Practices Are Systematically Eroding the Science Ethos: A Gate and Code Strategy to Minimise Their Operating Space and Restore Research Best Practice","authors":"Kenneth Timmis,&nbsp;Paul Williams,&nbsp;Zeynep Ceren Karahan,&nbsp;Purificación López-García,&nbsp;Paul Rainey,&nbsp;Max Chavarria,&nbsp;Chris Greening,&nbsp;Karen Steward,&nbsp;John E. Hallsworth,&nbsp;Cristina Silva Pereira,&nbsp;Rafael Giraldo,&nbsp;Willy Verstraete,&nbsp;Stipan Jonjić,&nbsp;Juan Luis Ramos,&nbsp;Olga Nunes,&nbsp;Antonio Ventosa,&nbsp;Rachel Armstrong,&nbsp;Angela Sessitsch,&nbsp;Eliora Ron,&nbsp;Hui Wang","doi":"10.1111/1751-7915.70180","DOIUrl":"10.1111/1751-7915.70180","url":null,"abstract":"&lt;p&gt;Scientific research seeks to extend knowledge and understanding, an activity that perhaps more than any other advances society and humanity. In essence, it is the search for truth. But, because it seeks new knowledge, there is little or no benchmark for appraisal of the plausibility or validity of the immediate conclusions drawn from new information gained, no instant confirmation. For this and other reasons, the science ethos requires the highest level of &lt;i&gt;rigour&lt;/i&gt; to ensure the highest level of probability that new findings are true, or at least the most plausible under the prevailing circumstances and state of knowledge. Research is only as good as its degree of &lt;i&gt;rigour&lt;/i&gt;. Rigour comes through intensive and comprehensive scientific training and mentoring that teaches critical and agnostic evaluation of new results, self-scrutiny and self-criticism. Additional rigour comes via independent scrutiny and validation: peer review of results and interpretations submitted as publications, and peer repetition of key experiments. However, the current proliferation of publication vehicles whose business model is based on maximisation of papers published, and the revenue stream of article processing charges (APCs) they generate, is promoting an insidious degradation of rigour and quality standards of reviewing–editing practices. Such &lt;i&gt;predatory practices&lt;/i&gt; result in the systematic degradation of research quality and its “truthfulness”. Moreover, they undermine the science ethos and threaten to create a new generation of scientists that lack this ethos. These trends will inevitably progressively erode public trust in scientists and the research ecosystem. This Editorial is a call for action to all actors, in particular leaders, in scientific research to oppose predatory practices in science dissemination—to restrict the operational space of those responsible for such practices—in order to restore and maintain research rigour and the science ethos and to prevent a downward spiral of research quality. It proposes two linked actionable solutions to the problem, one for the “pull” element of predatory practices and one for the “push” element of research ecosystem management practices, especially those promoting the &lt;i&gt;publish or perish&lt;/i&gt; mentality, that drive authors to publish in journals with predatory practices. To counter the “pull”, we propose a solution based on the principle of &lt;i&gt;prevention, rather than cure,&lt;/i&gt; and list a number of essential policy decisions and actions that should be taken at all levels of the science chain/cloud to achieve this. A central plank of the concept is &lt;i&gt;journal accreditation&lt;/i&gt;, without which a journal would be ineligible for payment of APCs from public funds. For accreditation, a journal would need to convincingly demonstrate adoption of a prescribed &lt;i&gt;journal code of conduct&lt;/i&gt;. Ideally, accreditation would also be required for inclusion in journal indexing and ranking services and bibliographic da","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Identification and Typing of Carbapenem-Resistant Klebsiella pneumoniae Using MALDI-TOF MS and Machine Learning","authors":"Zhiyi Ye,&nbsp;Jin Zhu,&nbsp;Yang Liu,&nbsp;Jun Lu","doi":"10.1111/1751-7915.70184","DOIUrl":"10.1111/1751-7915.70184","url":null,"abstract":"<p>Use matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) to screen the specific mass peaks of carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP), compare the differences in spectrum peaks between intestinal and bloodstream screening of CRKP, and assess the utility of MALDI-TOF MS in quickly identifying various CRKP sources. From 2014 to 2023, a total of 267 <i>Klebsiella pneumoniae</i> strains were collected at Quzhou People's Hospital, including 60 intestinal screening isolates from ICU patients and 207 bloodstream infection isolates. MALDI-TOF MS was used to profile peptides in CRKP and carbapenem-sensitive <i>Klebsiella pneumoniae</i> (CSKP), followed by analysis with flexAnalysis and ClinProTools 3.0. Statistically significant protein peaks were selected to build classification models, which were verified using non-duplicate strains. MALDI-TOF MS achieved &gt; 99.9% accuracy in identifying <i>Klebsiella pneumoniae</i>. Characteristic peaks (2523.43, 3041.62, 4520.11, 10,079.18 Da) were used to develop resistance analysis models, with the optimal model (SNN) showing 90.08% sensitivity, 95.80% specificity and identification accuracies of 90% for CSKP and 89.66% for CRKP. Another model using peaks (8876, 8993, 9139 Da) differentiated CRKP origins, with the ideal model (QC) achieving 86.85% sensitivity, 88.46% specificity, and accuracies of 81.82% for bloodstream and 95.00% for intestinal CRKP.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PFAS Biodegradation and the Constraints of Thermodynamics","authors":"Lawrence P. Wackett","doi":"10.1111/1751-7915.70181","DOIUrl":"10.1111/1751-7915.70181","url":null,"abstract":"<p>Microbial metabolism of PFAS such as perfluorooctanoic acid (PFOA) or perfluorooctanesulfonic acid (PFOS) would yield 15 toxic fluoride anions and metabolites with relatively low growth yield coefficients.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70181","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indole Derivatives Biosynthesis in Bifidobacterium longum subsp. infantis and the Tryptophan Substrate Availability","authors":"Shi-Min Zhang,&nbsp;Hui-Chu Wu,&nbsp;Jia-He Hung,&nbsp;Shir-Ly Huang","doi":"10.1111/1751-7915.70167","DOIUrl":"10.1111/1751-7915.70167","url":null,"abstract":"<p>The metabolic processes of <i>Bifidobacterium longum</i> subsp. <i>infantis</i>, an early coloniser of the human gut, are essential for gut health, mainly due to the production of indole derivatives from tryptophan. This study investigates the capacity of <i>B. infantis</i> ATCC 15697 to biosynthesise indole-3-lactate (ILA), indole-3-acetate (IAA), and indole-3-carboxaldehyde (I3CA) and the regulatory effects of substrate availability on these pathways. The tryptophan catabolic profile of <i>B. infantis</i> ATCC 15697 under a non-growing but metabolically active state was investigated. Through HPLC-PDA and LC–MS analyses, we confirmed for the first time the production of IAA and I3CA by <i>B. infantis</i> ATCC 15697. The results revealed a dose-dependent relationship between tryptophan availability and the production of indole derivatives, highlighting the nutrient-driven effect of these metabolic pathways. By integrating genomic analysis with metabolic profiles, we proposed potential pathways underlying the biosynthesis of IAA and I3CA from tryptophan. These findings enhance our understanding of the role of <i>B. infantis</i> ATCC 15697 in human health, with ILA, IAA, and I3CA contributing to immune modulation and gut health. We also provide a platform for using <i>B. infantis</i> ATCC 15697 as a biocatalyst for the biosynthesis of beneficial indole derivatives through whole-cell bioconversion, which was further demonstrated in <i>B. infantis</i> ATCC 25962 and ATCC 15702. Future in vivo studies will help clarify the impact of these metabolites on the gut environment and inform dietary and probiotic strategies for enhancing indole derivatives production.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Public Health Microbiome Curriculum: Looking Below the Tip of the Iceberg for Approaches to Population Health","authors":"Melissa K. Melby,&nbsp;Swapna Mylabathula,&nbsp;Meghan B. Azad,&nbsp;Sarah Turner,&nbsp;Naama Geva-Zatorsky,&nbsp;Carolina Tropini,&nbsp;Melissa B. Manus,&nbsp;Martin Blaser,&nbsp;Mark Nichter","doi":"10.1111/1751-7915.70160","DOIUrl":"10.1111/1751-7915.70160","url":null,"abstract":"<p>We discuss the opportunity for public health microbiome curricula to bridge the gaps in knowledge that exist between microbiome researchers and the lay public. We propose equipping public health professionals, important facilitators of public outreach and behaviour change, with three public health curriculum modules focused on breastfeeding, antibiotics and diet. These modules shift the focus from microbes as pathogens to potential partners in promoting health across the life course. Current public health messages cover only the ‘tip of the iceberg’ in exploring mechanisms, and this microbiome curriculum dives below the surface to provide fresh perspectives. These microbiome insights allow us to move beyond a focus on microbes as pathogens to understand the numerous collaborative roles played by the microbiome in producing health, and the upstream factors influencing the microbiome, thereby offering mechanistic insights that can be harnessed for public health education.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic-Based Approaches for Sustainable Control of Infectious Risk in Mass Transport: Current Data and Future Perspectives","authors":"Irene Soffritti,&nbsp;Maria D'Accolti,&nbsp;Francesca Bini,&nbsp;Eleonora Mazziga,&nbsp;Antonella Volta,&nbsp;Matteo Bisi,&nbsp;Sante Mazzacane,&nbsp;Elisabetta Caselli","doi":"10.1111/1751-7915.70177","DOIUrl":"10.1111/1751-7915.70177","url":null,"abstract":"<p>The built environments of high-traffic areas can play a significant role in the transmission of microorganisms and associated infections, sometimes favouring the selection of multidrug-resistant (MDR) organisms due to the excessive use of conventional disinfectants. Probiotic-based sanitation (PBS) was suggested as a novel alternative approach to control the infectious risk in crowded community environments due to its effectiveness in reducing fungal, bacterial, and viral pathogens in sanitary settings. PBS may thus trigger a paradigm shift from chemical to biological strategies in cleaning environments with high human occupancy, offering an ecological and economically sustainable alternative to conventional chemical disinfection. Providing robust data supporting the results reported so far, it has the potential to optimise bioburden control and infection prevention in mass transportation spaces. This review brings together existing research on PBS in mass transportation areas, pinpoints areas of lack of information, and explores its potential future uses, including the creation of probiotic-based materials for sustainable biocontrol in high-traffic areas.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70177","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methanol-Free Protein Expression in Komagataella phaffii With Magnetic or Non-Magnetic Heating","authors":"Ibrahim Dagci,&nbsp;Seyda Yildiz Arslan,&nbsp;Kubra Solak,&nbsp;Melek Acar,&nbsp;Yagmur Unver,&nbsp;Ahmet Mavi","doi":"10.1111/1751-7915.70183","DOIUrl":"10.1111/1751-7915.70183","url":null,"abstract":"<p><i>Komagataella phaffii</i> is among the most widely used expression systems, with methanol-inducible promoters being preferred for protein expression due to their stringent regulation and exceptional strength. However, the applicability of this system, particularly in food and pharmaceutical products, is limited by methanol's toxic and pro-inflammatory properties. Therefore, obtaining a novel methanol-free expression system is necessary. In this study, we obtained a novel expression plasmid, pHSPαA, carrying the <i>HSP70</i> promoter (<i>P</i>_<i>HSP70</i>) to regulate heterologous expression through heat induction. The extracellular expression of azurin was achieved using this methanol-free system under the control of <i>P</i>_<i>HSP70</i>, induced by either magnetic or non-magnetic heating. To enhance heat-induced expression, recombinant cells were immobilised with Fe₃O₄@PEI_{25 kDa} nanoparticles, which facilitated heat release under an AC magnetic field, thereby increasing cell permeability and protein secretion. A time-dependent increase in protein expression was observed in non-magnetic heating but not under magnetic heating. However, immobilised cells exhibited a higher protein secretion capacity compared to non-immobilised cells. These findings suggest that the novel methanol-free expression system represents a promising alternative for heterologous gene expression, particularly for the production of therapeutically relevant and food-grade recombinant proteins.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}