Microbial Biotechnology最新文献

{"title":"Akkermansia muciniphila Alleviates Olanzapine-Induced Hepatic Steatosis via the Gut Microbiota-IGFBP2/APOA1-Liver Axis","authors":"Jing Wang,&nbsp;Peiru Chen,&nbsp;Yahui Deng,&nbsp;Lixiu Yu,&nbsp;Chuyue Tu,&nbsp;Xiaojin Xu,&nbsp;Xiangming Fang,&nbsp;Weiyong Li","doi":"10.1111/1751-7915.70281","DOIUrl":"10.1111/1751-7915.70281","url":null,"abstract":"<p>Olanzapine is associated with a high risk of hepatic steatosis as a commonly used atypical antipsychotic. In this study, we observed differential susceptibility to olanzapine-induced fatty liver disease in both rats and patients. Notably, patients with olanzapine-induced liver damage exhibited an altered gut microbiota composition, with <i>Akkermansia muciniphila</i> showing the most pronounced alteration. To explore its therapeutic potential, we administered <i>A. muciniphila</i> to olanzapine-treated rats, which significantly reduced hepatic lipid accumulation and liver injury. Gut microbiome analysis revealed significant alterations in microbial diversity and composition following <i>A. muciniphila</i> treatment. Transcriptomic analysis further identified differentially expressed genes in the liver, highlighting the involvement of <i>IGFBP2</i> and <i>APOA1</i> in the protective effects of <i>A. muciniphila</i> . Functional validation demonstrated that overexpression of <i>IGFBP2</i> and <i>APOA1</i> alleviated olanzapine-induced hepatic steatosis in both cellular and animal models. These findings suggest that <i>A. muciniphila</i> exerts hepatoprotective effects via the gut microbiota-IGFBP2/APOA1-liver axis, offering a potential microbiota-targeted strategy to mitigate olanzapine-induced metabolic dysfunction.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 12","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145720228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Creative Futures in Education: Building ‘Imagination Infrastructures’ for Microbiology and Beyond","authors":"Jake M. Robinson,&nbsp;Martin F. Breed,&nbsp;Alexia Barrable,&nbsp;Ariane König,&nbsp;Robin Taylor,&nbsp;Kenneth Timmis","doi":"10.1111/1751-7915.70284","DOIUrl":"10.1111/1751-7915.70284","url":null,"abstract":"<p>Education is often reduced to the transmission of knowledge, yet in an era of climate disruption, biodiversity decline, and social injustice and unrest, learners require more than facts and skills. They must develop adaptive capacities that enable them to question, critically analyse, imagine, act, and empathise. One such fundamental capacity is <i>imagination</i>, which, despite its centrality to scientific discovery, is frequently undervalued in science education, particularly in fields considered ‘hard’ sciences. Microbiology offers a compelling context for better cultivating imagination because its study requires learners to visualise invisible worlds, connect them to ecological and human health, and explore how such knowledge might be applied to societal challenges. Here, we discuss the concept of <i>imagination infrastructures</i>—the environments, tools, practices, inner capacities, and symbolic resources that enable collective imagination—as a framework for better embedding imagination into microbiology education and beyond. We illustrate how imagination infrastructures can help democratise learning, expand worldviews, and promote a sense of responsibility, citizenship, and stewardship. Overcoming curricular, cultural, and resource barriers is required. By nurturing imagination as essential infrastructure, education can equip future microbiologists—and citizens more broadly—to navigate uncertainty and co-create regenerative futures.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 12","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70284","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145720201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Methanol Concentration on Recombinant Protein Glycosylation in Pichia pastoris SuperMan5","authors":"Andre Ohara,&nbsp;Zhang Pengyue,&nbsp;Xinqi Cao,&nbsp;Roberto Donini,&nbsp;Stuart M. Haslam,&nbsp;Karen M. Polizzi","doi":"10.1111/1751-7915.70272","DOIUrl":"10.1111/1751-7915.70272","url":null,"abstract":"<p>The methylotrophic yeast <i>Pichia pastoris</i> (also known as <i>Komagataella phaffii</i>) is a prominent platform for recombinant protein production, offering benefits such as thermo- and osmotolerance, high-density growth, and efficient protein secretion. Its ability to metabolise methanol, an increasingly available carbon source, enhances its cost-effectiveness and sustainability for industrial use. As a eukaryotic host, <i>P. pastoris</i> ensures proper protein folding and post-translational modifications (PTMs), including glycosylation, which is essential for correct folding and endoplasmic reticulum (ER) quality control. While ER-transferred glycans are critical for maturation, additional modification in the Golgi apparatus can yield larger glycans whose impact on stability, solubility, and bioactivity may be either beneficial or undesirable, depending on the application of the heterologous protein. The impact of induction conditions on glycosylation of proteins secreted by <i>P. pastoris</i> SuperMan5 was examined, using the DS-1 (G2P[4]) and WA (G1P[8]) VP8* rotavirus capsid proteins as a model. An ELISA-based screening system was employed for clone selection and media optimization, with results showing easy integration into automated workflows. Methanol concentration was found to impact both <i>N</i>- and <i>O-</i>linked glycosylation complexity, shaping the glycosylation profile of the target protein as well as the <i>P. pastoris</i> secretome. This study underscores the importance of optimising cultivation conditions to enhance protein yield, refine glycosylation, and minimise impurities, all of which are crucial for large-scale production and efficient downstream processing. It also suggests a method for easy modulation of glycosylation depending on the target application and the desired level of glycosylation.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 12","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70272","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Halogenated Indoles as Antimicrobial and Antivirulence Agents Against Drug-Resistant Staphylococcus aureus","authors":"MinHwi Sim,&nbsp;Bharath Reddy Boya,&nbsp;Yong-Guy Kim,&nbsp;Jin-Hyung Lee,&nbsp;Jintae Lee","doi":"10.1111/1751-7915.70280","DOIUrl":"10.1111/1751-7915.70280","url":null,"abstract":"<p>The emergence of multidrug-resistant <i>Staphylococcus aureus</i> requires the development of novel agents that can target both planktonic cells and persistent biofilms. In this study, a library of multi-halogenated indoles was evaluated for antibacterial, antibiofilm and antivirulence activities against <i>S. aureus</i> including methicillin-resistant strains. Two lead compounds, 6-bromo-4-iodoindole and 4-bromo-6-chloroindole, exhibited potent bactericidal activity (MIC = 20–30 μg/mL) comparable to the antibiotic gentamicin, effectively inhibited biofilm formation and persister formation and suppressed key virulence haemolysis. These effects were associated with intracellular ROS generation and transcriptional downregulation of quorum-sensing genes of <i>agrA</i> and <i>RNAIII</i> and virulence genes of <i>hla</i> and <i>nuc1</i>. Also, 6-bromo-4-iodoindole synergised with aminoglycoside tobramycin and gentamicin, significantly reducing their effective MICs. Notably, these two multi-halogenated indoles did not induce drug resistance for 20 days while gentamicin rapidly increased drug resistance. Cytotoxicity assays in HepG2 cells and phytotoxicity tests confirmed a favourable safety profile. Structure–activity relationship identified multi-halogenation at the C4, C5, C6 and C7 positions of indole as favourable for enhanced activities and also suggested that more halogens could improve the activities. This study highlights multi-halogenated indoles as promising multi-target antimicrobial agents with potential therapeutic and environmental applications against <i>S. aureus</i>, including drug-resistant and biofilm-forming strains.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 12","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12673945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145666410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Potential of Lentilactobacillus hilgardii in Malolactic Fermentation: Comparative Genomics and Fermentation Dynamics","authors":"Giacomo Mantegazza,&nbsp;Nicola Mangieri,&nbsp;Elnaz Vojoudi Yazdi,&nbsp;Pasquale Russo,&nbsp;Diego Mora,&nbsp;Giorgio Gargari","doi":"10.1111/1751-7915.70259","DOIUrl":"10.1111/1751-7915.70259","url":null,"abstract":"<p>This study aimed to assess the potential of <i>Lentilactobacillus hilgardii</i> as a novel candidate for malolactic fermentation (MLF) in winemaking, through comparative genomics and experimental validation, in direct comparison with <i>Oenococcus oeni</i>. We performed a pangenome analysis on 16 <i>L. hilgardii</i> and 7 <i>O. oeni</i> strains to explore their genetic diversity, focusing on wine-related traits. Functional predictions were generated using genome-scale metabolic models (ModelSEED/KBase), including in silico co-inoculation with <i>Saccharomyces cerevisiae</i> EC1118 and post-alcoholic fermentation simulations. The reference strains <i>L. hilgardii</i> DSM 20176 and <i>O. oeni</i> DSM 20252 were experimentally tested for MLF performance in a synthetic wine-like medium at 25°C and 10°C. Core-genome comparison revealed that 67.9% of the malolactic enzyme sequence is conserved between the two species, with comparable docking affinity to L-malic acid. <i>L. hilgardii</i> harboured unique enzymes with potential oenological interest (phenolic acid decarboxylase, mannitol dehydrogenase, glucosidase) and distinctive stress-related proteins (YaaA, HrcA, ASP23), suggesting improved tolerance to oxidative, temperature, and alkaline stresses. Notably, <i>L. hilgardii</i> showed genomic potential to degrade putrescine, arginine, and ornithine, precursors of ethyl carbamate. Experimentally, <i>L. hilgardii</i> reduced L-malic acid from 2.5 g/L to &lt; 0.1 g/L within 12 days at 10°C, while <i>O. oeni</i> showed no MLF activity at this temperature. At 25°C, both strains completed MLF within 6–7 days. <i>L. hilgardii</i> also consumed &gt; 80% of residual fructose at 10°C, whereas <i>O. oeni</i> showed minimal utilisation. Our results demonstrate that <i>L. hilgardii</i> combines a favourable genomic repertoire for wine adaptation with superior MLF performance at low temperature, suggesting its potential as an alternative to <i>O. oeni</i> in cool-climate winemaking. This work provides the first genome-scale comparative and functional evaluation of <i>L. hilgardii</i> in the winemaking context, highlighting its technological promise to improve fermentation reliability, reduce spoilage risk, and expand the biodiversity of malolactic starters.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 12","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145627420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic Insights Into the Ecology, Taxonomy and Metabolic Capabilities of ‘Candidatus Darwinibacteriales’ Ord. Nov. (Formerly MBA03), a Potential Key Player in Anaerobic Digestion","authors":"Roser Puchol-Royo,&nbsp;Javier Pascual,&nbsp;Asier Ortega-Legarreta,&nbsp;Pascal Otto,&nbsp;Jeroen Tideman,&nbsp;Sjoerd-Jan de Vries,&nbsp;Christian Abendroth,&nbsp;Kristie Tanner,&nbsp;Manuel Porcar,&nbsp;Adriel Latorre-Perez","doi":"10.1111/1751-7915.70258","DOIUrl":"10.1111/1751-7915.70258","url":null,"abstract":"<p>Biogas, a mix of CO₂, CH₄ and small proportions of other gases, is a biofuel obtained by anaerobic digestion (AD). Biogas production is often considered a black box process, as the role and dynamics of some of the microorganisms involved remain undisclosed. Previous metataxonomic studies in the frame of the MICRO4BIOGAS project (www.micro4biogas.eu) revealed that MBA03, an uncharacterised and uncultured bacterial taxon belonging to phylum <i>Bacillota</i>, was very prevalent and abundant in industrial full-scale AD plants. Despite the efforts, this taxon has not yet been cultivated, which makes the analysis of its taxonomy, ecology and metabolism even more challenging. In the present work, 30 samples derived from anaerobic digesters were sequenced, allowing the reconstruction of 108 metagenome-assembled genomes (MAGs) potentially belonging to MBA03. According to phylogenetic analyses and genomic similarity indices, MBA03 was classified as a new bacterial order, proposed as ‘<i>Candidatus</i> Darwinibacteriales’ ord. nov., which includes ‘<i>Candidatus</i> Darwinibacter acetoxidans’ gen. nov., sp. nov. of ‘<i>Candidatus</i> Darwinibacteriaceae’ fam. nov., along with ‘<i>Candidatus</i> Wallacebacter cryptica’ gen. nov., sp. nov. of the ‘<i>Candidatus</i> Wallacebacteriaceae’ fam. nov. Ecotaxonomic studies determined that AD processes are the main ecological niche of ‘<i>Candidatus</i> Darwinibacteriales’. Moreover, metabolic predictions identified <i>Darwinibacteraceae</i> members as putative syntrophic acetate-oxidising bacteria (SAOB), as they encode for the reversed Wood–Ljungdahl (W–L) pathway coupled to the glycine cleavage system. This suggests that <i>Darwinibacteraceae</i> members could work in collaboration with hydrogenotrophic methanogenic archaea to produce methane in industrial biogas plants. Overall, our findings present ‘<i>Candidatus</i> Darwinibacteriales’ as a potential key player in anaerobic digestion and pave the way towards the complete characterisation of this newly described bacterial taxon, which has not yet been cultured.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 12","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70258","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mining Actinomycetes' Metabolomes and Genomes for Anti-Phytophthora infestans Compounds","authors":"Ola Abdelrahman,&nbsp;Quinn Coxon,&nbsp;Eliane Abou-Mansour,&nbsp;Floriane L'Haridon,&nbsp;Laurent Falquet,&nbsp;Pierre-Marie Allard,&nbsp;Laure Weisskopf","doi":"10.1111/1751-7915.70269","DOIUrl":"10.1111/1751-7915.70269","url":null,"abstract":"<p>Actinomycetes are well-known for producing a diverse array of specialised metabolites with various bioactivities; yet, identifying metabolites with targeted activity against specific pathogens remains challenging. In this study, we employed a comparative metabolomic and genomic approach on 63 actinomycete strains differing in their ability to inhibit or alter the mycelial growth of <i>Phytophthora infestans</i>, the causal agent of potato late blight. This comparative approach efficiently pinpointed approximately 1000 mass spectrometry features linked to active extracts, out of 16,500 detected features. Our analysis putatively identified over 75 compounds with potential activity against <i>P. infestans</i>, including borrelidin, actinomycin D, antimycin A, macbecin I, myriocin and ikarugamycin. Our study shows that leveraging multi-omics analysis of phylogenetically related strains with differential activity is a promising strategy which, combined with a relatively high throughput metabolite extraction method, advanced mass spectrometry and cutting-edge tools for bacterial metabolite annotation and prediction, allowed a straightforward selection of interesting candidate compounds for the biological control of an important plant pathogen such as <i>P. infestans</i>. The methodology outlined here offers broader applicability for identifying bioactive compounds underlying any phenotype of interest, provided this phenotype varies in phylogenetically closely related strains.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 12","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70269","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol of Root-Knot Nematodes via siRNA-Loaded Extracellular Vesicles From a Nematophagous Fungus Arthrobotrys oligospora","authors":"Xinyi Huang,&nbsp;Wenliang Zhou,&nbsp;Xiaoying Liu,&nbsp;Ke-Qin Zhang,&nbsp;Juan Li","doi":"10.1111/1751-7915.70274","DOIUrl":"10.1111/1751-7915.70274","url":null,"abstract":"<p>Root-knot nematodes (<i>Meloidogyne</i> spp.) represent a major threat to global crop production, and current chemical nematicides pose serious environmental and health risks. RNA interference (RNAi) offers a promising gene-specific strategy for nematode control. However, the efficient and sustainable delivery of RNA molecules into nematodes remains a significant challenge. In this study, we developed an innovative RNA delivery platform using extracellular vesicles (EVs) derived from the nematode-trapping fungus <i>Arthrobotrys oligospora</i>. EVs were either exogenously loaded with synthetic siRNAs targeting the <i>Mi-flp-18</i> gene of <i>M. incognita</i> or harvested from engineered fungal strains expressing short hairpin RNAs (shRNAs) or double-stranded RNAs (dsRNAs) against multiple nematode neuropeptide genes (<i>flp</i> and <i>nlp</i> families). The engineered EVs efficiently delivered RNA cargos into nematodes, leading to significant downregulation of target gene expression. Functional assays and greenhouse experiments revealed the biocontrol potential of the engineered fungal strains, with reductions in nematode motility, root invasion and infectivity. This is the first demonstration in a nematophagous fungus that EVs can serve as effective RNA delivery vehicles for the control of root-knot nematodes. The use of engineered <i>A. oligospora</i> strains provides a scalable, eco-friendly alternative to synthetic delivery systems and transgenic crops. Our findings establish fungal EVs as a powerful tool in cross-kingdom RNAi applications and open new avenues for sustainable pest management in agriculture.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 11","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70274","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145595473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycosylated Foot-And-Mouth Disease Virus-Like Particles Produced in Pichia Pastoris Enhance Stability and Immunogenicity","authors":"Zhiyao Li,&nbsp;Hu Dong,&nbsp;Shuanghui Yin,&nbsp;Manyuan Bai,&nbsp;Zhidong Teng,&nbsp;Lingbo Chen,&nbsp;Suyu Mu,&nbsp;Yun Zhang,&nbsp;Yaozhong Ding,&nbsp;Shiqi Sun,&nbsp;Huichen Guo","doi":"10.1111/1751-7915.70271","DOIUrl":"10.1111/1751-7915.70271","url":null,"abstract":"<p>Despite the availability of vaccines, foot-and-mouth disease (FMD) remains a significant concern in many developing countries, causing severe economic losses and affecting local farming communities. Virus-like particle (VLP) vaccines are highly regarded for their safety and efficacy. N-glycosylation for stabilisation and recognition by antigen-presenting cells has been a widely adopted strategy, particularly in enveloped viruses. Here, FMD virus (FMDV) VLPs were employed as a model for artificial glycosylation. N-glycosylation was introduced by mutating the potential glycosylation site of VP1 and then N-glycosylated FMDV VLPs were successfully produced in <i>Pichia pastoris</i>. Glycan profiling revealed that the majority of associated glycans (72.93%) were of the high-mannose type, with additional hybrid type (4.16%) and complex type (22.92%) detected. Functional analyses demonstrated that glycosylation significantly enhanced the stability of VLPs and facilitated the uptake by antigen-presenting cells. Animal experiments further revealed that glycosylation could induce a higher cellular immune response compared to WT VLPs, offering a reference for the glycosylation design of VLP vaccines.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 11","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12642819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145585561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Origin, Role and Fate of the Denaturant Guanidine","authors":"Antoine Danchin,&nbsp;Victor de Lorenzo,&nbsp;Pablo Iván Nikel,&nbsp;Conghui You","doi":"10.1111/1751-7915.70266","DOIUrl":"10.1111/1751-7915.70266","url":null,"abstract":"<p>Despite its unusual structure and detrimental role as a chaotropic guanidinium ion, guanidine [HNC(NH₂)₂] exists as a genuine metabolite in many microbes, and its negative effects are mitigated by specific exporters. The metabolic origin of this molecule remains unknown, except in a few cases. We propose here that it results from the deep oxidation of guanine-containing nucleotides derived from 8-oxoguanine in the presence of molecular oxygen. Analysis of the co-evolutionary patterns of guanidine exporters in distant bacteria, together with the analysis of operons involved in purine catabolism, revealed that although purines are generally broken down to urea, guanidine can be produced instead in the presence of molecular oxygen. We investigated how this process could enable guanidine to play a distinct regulatory role in directing metabolism in the presence of molecular oxygen. We propose that it is used as a signal meant to control the generation of reactive oxygen species at an optimal level for the cell.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 11","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12623156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145538506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}