Microbial Biotechnology最新文献_第10页

Assessing the transcriptional landscape of Pseudomonas phage 201ϕ2-1: Uncovering the small regulatory details of a giant phage 评估假单胞菌噬菌体 201j2-1的转录景观：揭示巨型噬菌体的微小调控细节。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-26 DOI: 10.1111/1751-7915.70037

Jorien Poppeliers, Mathijs Focquet, Maarten Boon, Marjan De Mey, Julie Thomas, Rob Lavigne

{"title":"Assessing the transcriptional landscape of Pseudomonas phage 201ϕ2-1: Uncovering the small regulatory details of a giant phage","authors":"Jorien Poppeliers, Mathijs Focquet, Maarten Boon, Marjan De Mey, Julie Thomas, Rob Lavigne","doi":"10.1111/1751-7915.70037","DOIUrl":"10.1111/1751-7915.70037","url":null,"abstract":"The transcriptional architecture of phages can deepen our understanding of the phage-host infection process and can be of key importance for phage engineering and biotechnological applications. Here, we applied ONT-cappable-sequencing, a long-read RNA-sequencing technique, to study the regulatory mechanisms of Pseudomonas infecting giant phage 201ϕ2-1. We identified 67 promoters and 132 terminators that together represent 92 transcriptional units. A full comparison of these data to the transcriptome of model Pseudomonas phage ϕKZ confirmed that the transcriptional programs of these prototypes of the Serwervirus and Phikzvirus genera are largely conserved, despite some subtle regulatory differences. Evidence supporting these shared mechanisms include the identification of highly similar sequence motifs for regulatory elements in both phages and the conservation of regulatory elements loci relative to homologous genes in each phage. Moreover, we discovered a sRNA in 201ϕ2-1 that is highly conserved among prototype members of different giant phage genera. Sequencing of the 201ϕ2-1 host genome resulted in its reclassification as Pseudomonas atacamensis, a close relative of the important agricultural biocontrol agent Pseudomonas chlororaphis. Finally, we conducted in vivo assays of eight 201ϕ2-1 terminators and found them to strongly terminate transcription in P. chlororaphis. Control elements from phage transcriptional programs have a rich history for applications in biotechnology. In these studies, we demonstrate new insight into the transcriptional program of 201ϕ2-1 and demonstrate the potential of its regulatory elements for novel and useful tools for synthetic biology circuitry.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 10","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum antibodies against mimotopes of Merkel cell polyomavirus oncoproteins detected by a novel immunoassay in healthy individuals and Merkel cell carcinoma patients 用新型免疫测定法检测健康人和梅克尔细胞癌患者血清中针对梅克尔细胞多瘤病毒肿瘤蛋白拟态的抗体。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-25 DOI: 10.1111/1751-7915.14536

Chiara Mazziotta, Giada Badiale, Christian Felice Cervellera, Giulia Tonnini, Milena Oimo, Antoine Touzé, Françoise Arnold, Stefania Zanussi, Ornella Schioppa, Giuseppe Fanetti, Mauro Tognon, Fernanda Martini, John Charles Rotondo

{"title":"Serum antibodies against mimotopes of Merkel cell polyomavirus oncoproteins detected by a novel immunoassay in healthy individuals and Merkel cell carcinoma patients","authors":"Chiara Mazziotta, Giada Badiale, Christian Felice Cervellera, Giulia Tonnini, Milena Oimo, Antoine Touzé, Françoise Arnold, Stefania Zanussi, Ornella Schioppa, Giuseppe Fanetti, Mauro Tognon, Fernanda Martini, John Charles Rotondo","doi":"10.1111/1751-7915.14536","DOIUrl":"10.1111/1751-7915.14536","url":null,"abstract":"Merkel cell polyomavirus (MCPyV) is the foremost causative factor of Merkel cell carcinoma (MCC), a rare yet highly aggressive skin cancer. Although the evaluation of circulating IgG antibodies against Merkel cell polyomavirus (MCPyV) LT/sT oncoproteins is clinically useful for MCC diagnosis/prognosis, a limited number of assays for identifying such antibodies have been developed. Herein, a novel indirect immunoassay with synthetic epitopes/mimotopes of MCPyV oncoproteins was computationally designed and experimentally validated on control sera and sera from healthy individuals and MCC patients. Upon computational design of five synthetic peptides, the performance of the immunoassay in detecting anti-oncoprotein IgGs in MCPyV-positive and -negative control sera was evaluated. The immunoassay was afterwards extended on sera from healthy individuals, and, for longitudinal analysis, MCC patients. Performance properties such as sensitivity and specificity and positive/negative predictive values were adequate. Receiver-operating characteristic (ROC) curves indicated that the areas under the curves (AUCs) were within the low/moderately accurate ranges. Immunoassay was repeatable, reproducible and accurate. As expected, the serum anti-oncoprotein IgG prevalence in healthy individuals was low (2%–5%). Anti-oncoprotein IgGs slightly increased when MCC patients experienced partial tumour remission and/or stable disease, compared to baseline. Our data indicate that the newly developed immunoassay is reliable for detecting circulating anti-oncoprotein IgGs both in healthy individuals and MCC patients.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 10","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivating complexity: Advancements in establishing in vitro models for the mucus-adhering gut microbiota 培养复杂性：建立粘液粘附肠道微生物群体外模型的进展。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-22 DOI: 10.1111/1751-7915.70036

Marco Calvigioni, Diletta Mazzantini, Francesco Celandroni, Giovanni Vozzi, Emilia Ghelardi

引用次数: 0

Emerging strategies for treating medical device and wound-associated biofilm infections 治疗医疗器械和伤口相关生物膜感染的新策略。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-21 DOI: 10.1111/1751-7915.70035

Chenlong Wang, Yajuan Su, S. M. Shatil Shahriar, Yu Li, Jingwei Xie

{"title":"Emerging strategies for treating medical device and wound-associated biofilm infections","authors":"Chenlong Wang, Yajuan Su, S. M. Shatil Shahriar, Yu Li, Jingwei Xie","doi":"10.1111/1751-7915.70035","DOIUrl":"10.1111/1751-7915.70035","url":null,"abstract":"Bacterial infections represent a significant global threat to human health, leading to considerable economic losses through increased healthcare costs and reduced productivity. One major challenge in treating these infections is the presence of biofilms - structured bacterial communities that form protective barriers, making traditional treatments less effective. Additionally, the rise of antibiotic-resistant bacteria has exacerbated treatment difficulties. To address these challenges, researchers are developing and exploring innovative approaches to combat biofilm-related infections. This mini-review highlights recent advancements in the following key areas: surface anti-adhesion technologies, electricity, photo/acoustic-active materials, endogenous mimicking agents, and innovative drug delivery systems. These strategies aim to prevent biofilm formation, disrupt existing biofilms, and enhance the efficacy of antimicrobial treatments. Currently, these approaches show great potential for applications in medical fields such as medical device and wound – associated biofilm infections. By summarizing these developments, this mini-review provides a comprehensive resource for researchers seeking to advance the management and treatment of biofilm-associated infections.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 10","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillus helveticus attenuates alcoholic liver injury via regulation of gut microecology in mice 螺旋乳杆菌通过调节小鼠肠道微生态减轻酒精性肝损伤

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-21 DOI: 10.1111/1751-7915.70016

Jiawen Lv, Guanjing Lang, Qiangqiang Wang, Wenlong Zhao, Ding Shi, Ziyuan Zhou, Yangfan Shen, He Xia, Shengyi Han, Lanjuan Li

{"title":"Lactobacillus helveticus attenuates alcoholic liver injury via regulation of gut microecology in mice","authors":"Jiawen Lv, Guanjing Lang, Qiangqiang Wang, Wenlong Zhao, Ding Shi, Ziyuan Zhou, Yangfan Shen, He Xia, Shengyi Han, Lanjuan Li","doi":"10.1111/1751-7915.70016","DOIUrl":"10.1111/1751-7915.70016","url":null,"abstract":"Previous reports have demonstrated that alcohol consumption significantly reduces the abundance of Lactobacillus in the gut. In this study, we selected five species of the genus Lactobacillus, commonly found in fermented foods, and acknowledged them as safe, edible, and effective in preventing or treating certain diseases, to evaluate their effects on alcoholic liver disease (ALD). By comparing the liver damage indices in each group, we found that the type strain of Lactobacillus helveticus (LH, ATCC 15009) had the most marked alleviating effect on ALD-induced liver injury. Furthermore, experiments combining microbiomics and metabolomics were conducted to explore the mechanisms underlying the hepatoprotective effects of LH. Finally, we discovered that LH mitigated ethanol-induced liver steatosis and inflammation in ALD mice by altering the structure and function of the gut microbiome, increasing intestinal levels of short-chain fatty acids (SCFAs), and enhancing gut barrier integrity. These findings suggest a potential strategy for the clinical management of patients with ALD.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 10","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12 promote infected wound healing via regulation of the wound microenvironment 鼠李糖乳杆菌 GG 和动物双歧杆菌亚种 BB-12 通过调节伤口微环境促进感染伤口愈合

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-18 DOI: 10.1111/1751-7915.70031

Zhe Yin, Yilin Wang, Xiaojuan Feng, Changqing Liu, Xiaoyang Guan, Shuyan Liu, Zhanyi Long, Zhonghua Miao, Fang He, Ruyue Cheng, Yanting Han, Ka Li

{"title":"Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12 promote infected wound healing via regulation of the wound microenvironment","authors":"Zhe Yin, Yilin Wang, Xiaojuan Feng, Changqing Liu, Xiaoyang Guan, Shuyan Liu, Zhanyi Long, Zhonghua Miao, Fang He, Ruyue Cheng, Yanting Han, Ka Li","doi":"10.1111/1751-7915.70031","DOIUrl":"10.1111/1751-7915.70031","url":null,"abstract":"Infected wounds can result in complex clinical complications and delayed healing, presenting a significant global public health challenge. This study explored the effects of topical application of two probiotics, Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subsp. lactis BB-12, on the microenvironment of infected wounds and their impact on wound healing. LGG and BB-12 were applied separately and topically on the Staphylococcus aureus (S. aureus)-infected skin wounds of the rat model on a daily basis. Both probiotics significantly accelerated wound healing, demonstrated by enhanced granulation tissue formation and increased collagen deposition, with BB-12 showing superior efficacy. LGG and BB-12 both effectively inhibited neutrophil infiltration and decreased the expression of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Notably, BB-12 markedly reduced IL-6 levels, while LGG significantly lowered TNF-α, transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF). Additionally, both probiotics promoted macrophage polarization towards the anti-inflammatory M2 phenotype. Microbiota analysis revealed that LGG and BB-12 significantly decreased the abundance of pathogenic bacteria (e.g. Staphylococcus and Proteus) and increased the proportion of beneficial bacteria (e.g. Corynebacterium). Particularly, BB-12 was more effective in reducing Staphylococcus abundance, whereas LGG excelled in promoting Corynebacterium growth. These findings suggest the ability of LGG and BB-12 to modulate the wound microenvironment, enhance wound healing and provide valuable insights for the management of infected wounds.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 10","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flagellate bacteria-mediated tumour antigen delivery: A novel approach to enhance dendritic cell activation for in situ cancer vaccination 鞭毛细菌介导的肿瘤抗原递送：增强树突状细胞活化以进行原位癌症疫苗接种的新方法

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-18 DOI: 10.1111/1751-7915.70028

Wen Xia, Jinhui Wu

{"title":"Flagellate bacteria-mediated tumour antigen delivery: A novel approach to enhance dendritic cell activation for in situ cancer vaccination","authors":"Wen Xia, Jinhui Wu","doi":"10.1111/1751-7915.70028","DOIUrl":"https://doi.org/10.1111/1751-7915.70028","url":null,"abstract":"In situ vaccination is a therapeutic approach aimed at exploiting tumour antigens available at a tumour site to induce tumour-specific adaptive immune responses. Antigens released from dying tumour cells are assumed to be taken up by activated dendritic cells and presented to T cells that seek out and destroy tumour cells. This process is significantly impeded in the immunosuppressive microenvironment of tumours. There is a growing trend in in situ vaccine strategies that utilize bacteria as natural adjuvants or as factories for cytokines, aiming to enhance the presentation of in situ antigens by antigen-presenting cells. Recently, a novel approach using flagellate bacteria-mediated antigen delivery to activate dendritic cells has been proposed. This method actively facilitates the delivery of intratumoral antigens, improving their presentation for in situ cancer vaccination. Here, we highlight how flagellate bacteria-mediated antigen delivery enhances the immune activation capabilities of in situ vaccines. Meanwhile, we provide perspectives and outlooks on these promising antigen delivery technologies.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 10","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production-optimized fermentation of antifungal compounds by bacillus velezensis LZN01 and transcriptome analysis velezensis LZN01 杆菌抗真菌化合物的生产优化发酵及转录组分析

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-17 DOI: 10.1111/1751-7915.70026

Jiale Hu, Zhigang Wang, Weihui Xu

{"title":"Production-optimized fermentation of antifungal compounds by bacillus velezensis LZN01 and transcriptome analysis","authors":"Jiale Hu, Zhigang Wang, Weihui Xu","doi":"10.1111/1751-7915.70026","DOIUrl":"https://doi.org/10.1111/1751-7915.70026","url":null,"abstract":"Fusarium wilt is one of the major constraints on global watermelon production, and Fusarium oxysporum f. sp. niveum (Fon) is the causative agent of Fusarium wilt in watermelon and results in severe yield and quality losses worldwide. The enhancement of antifungal activity from antagonistic bacteria against Fon is highly practical for managing Fusarium wilt in watermelon. The aim of this study was to maximize the antifungal activity of Bacillus velezensis LZN01 by optimizing fermentation conditions and analysing its regulatory mechanism via transcriptome sequencing. The culture and fermentation conditions for strain LZN01 were optimized by single-factor and response surface experiments. The optimum culture conditions for this strain were as follows: the addition of D-fructose at 35 g/L and NH4Cl at 5 g/L in LB medium, pH 7, and incubation at 30°C for 72 h. The fungal inhibition rate for strain LZN01 reached 71.1%. The improvement of inhibition rate for strain LZN01 in optimization fermentation was supported by transcriptomic analysis; a total of 491 genes were upregulated, while 736 genes were downregulated. Transcriptome analysis revealed that some differentially expressed genes involved in carbon and nitrogen metabolism, oxidation–reduction, fatty acid and secondary metabolism; This optimization process could potentially lead to significant alterations in the production levels and types of antimicrobial compounds by the strain. Metabolomics and UPLC/Q-Exactive Orbitrap MS analysis revealed that the production yields of antimicrobial compounds, such as surfactin, fengycin, shikimic acid, and myriocin, increased or were detected in the cell-free supernatant (CFS) after the fermentation optimization process. Our results indicate that fermentation optimization enhances the antifungal activity of the LZN01 strain by influencing the expression of genes responsible for the synthesis of antimicrobial compounds.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 10","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142447720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-engineered bacteria in drug development 药物开发中的表面工程细菌

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-15 DOI: 10.1111/1751-7915.70033

Charles Dahlsson Leitao, Stefan Ståhl, John Löfblom

引用次数: 0

Escherichia coli on colorectal cancer: A two-edged sword 大肠杆菌对大肠癌的影响：一把双刃剑

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-10-14 DOI: 10.1111/1751-7915.70029

Chu Jian, Wu Yinhang, Zhuang Jing, Qu Zhanbo, Wang Zefeng, Han Shuwen

{"title":"Escherichia coli on colorectal cancer: A two-edged sword","authors":"Chu Jian, Wu Yinhang, Zhuang Jing, Qu Zhanbo, Wang Zefeng, Han Shuwen","doi":"10.1111/1751-7915.70029","DOIUrl":"https://doi.org/10.1111/1751-7915.70029","url":null,"abstract":"Escherichia coli (E. coli) is a ubiquitous symbiotic bacterium in the gut, and the diversity of E. coli genes determines the diversity of its functions. In this review, the two-edged sword theory was innovatively proposed. For the question ‘how can we harness the ambivalent nature of E. coli to screen and treat CRC?’, in terms of CRC screening, the variations in the abundance and subtypes of E. coli across different populations present an opportunity to utilise it as a biomarker, while in terms of CRC treatment, the natural beneficial effect of E. coli on CRC may be limited, and engineered E. coli, particularly certain subtypes with probiotic potential, can indeed play a significant role in CRC treatment. It seems that the favourable role of E. coli as a genetic tool lies not in its direct impact on CRC but its potential as a research platform that can be integrated with various technologies such as nanoparticles, imaging methods, and synthetic biology modification. The relationship between gut microflora and CRC remains unclear due to the complex diversity and interaction of gut microflora. Therefore, the application of E. coli should be based on the ‘One Health’ view and take the interactions between E. coli and other microorganisms, host, and environmental factors, as well as its own changes into account. In this paper, the two-edged sword role of E. coli in CRC is emphasised to realise the great potential of E. coli in CRC screening and treatment.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 10","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0