Microbial Biotechnology最新文献

{"title":"Lineage-Specific Adaptive Strategies Shape the Response of de Novo Lager Yeast Hybrids to High-Gravity Fermentation","authors":"Sonia Albillos-Arenal,&nbsp;Vasni Zavaleta,&nbsp;Fernanda Jeria,&nbsp;Darío González-Venegas,&nbsp;Tomás A. Peña,&nbsp;Pablo Villarreal,&nbsp;Francisco A. Cubillos","doi":"10.1111/1751-7915.70375","DOIUrl":"10.1111/1751-7915.70375","url":null,"abstract":"<p>Lager beer is the most widely consumed fermented beverage worldwide and is produced by the yeast <i>Saccharomyces pastorianus</i>, which originated from hybridization between <i>S. cerevisiae</i> (<i>Sc</i>) and <i>S. eubayanus (Se)</i>. Although the genetic factors underlying fermentation performance in <i>de novo</i> hybrids are well understood, how chimeric genomes evolve in relation to the parental background and stabilize under industrial stresses remains unclear. Here, we subjected representative novel hybrids derived from distinct parental lineages to long-term experimental evolution for 250 generations under conditions mimicking high-gravity brewing. We observed a clear pattern of the “rule of declining adaptability”, driven by diminishing-returns epistasis, whereby hybrids with lower initial fitness exhibited the greatest evolutionary gains. Overall, 38 of 69 evolved lines produced more CO₂ than their ancestral strains. Adaptive trajectories were strongly influenced by the <i>Sc</i> genetic background, while the <i>Se</i> contribution appeared comparatively limited, highlighting the dominant role of the <i>Sc</i> subgenome in determining brewing performance and evolvability. Genomic analysis of representative hybrids revealed different evolutionary strategies. The evolved HB3-I line (beer <i>Sc</i> parent) showed a large-scale chromosomal rearrangement with few coding-sequence changes, consistent with fine-tuning of an already adapted background. In contrast, HB44-II (bioethanol <i>Sc</i> parent) accumulated multiple gene-disruptive mutations affecting multiple pathways, indicating broader regulatory rewiring. These genomic changes were accompanied by transcriptional shifts in carbon metabolism, nutrient-responsive pathways and membrane-related functions. Together, our results provide a controlled framework for replaying yeast domestication, demonstrating that the adaptive potential of synthetic lager hybrids depends on their lineage, which is mainly shaped by the <i>S. cerevisiae</i> parental background.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13176630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147939392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148078293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148074463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutibacterium acnes–Derived Extracellular Vesicles Promote Epithelial Ovarian Cancer Progression by Activating the KEAP1–NRF2 Antioxidant Pathway to Suppress Ferroptosis","authors":"Qifa Huang,&nbsp;Qi Chen,&nbsp;Wenjie Xiong,&nbsp;Yuexi Sun,&nbsp;Yuxiong Huang,&nbsp;Ang Dai,&nbsp;Jianying Chen,&nbsp;Xue Wu,&nbsp;Ying Jiang,&nbsp;Fen Wei,&nbsp;Qi Chen,&nbsp;Tingtao Chen","doi":"10.1111/1751-7915.70373","DOIUrl":"10.1111/1751-7915.70373","url":null,"abstract":"<p>Bacterial extracellular vesicles are increasingly recognized as important mediators of microbe–host communication, yet their functional roles within tumour-associated microbiota remain poorly understood. Here, we investigated whether extracellular vesicles derived from <i>Cutibacterium acnes</i> (CEVs) regulate host redox metabolism and ferroptosis in epithelial ovarian cancer (EOC). Using integrated in vitro and in vivo models, we found that CEVs significantly promoted tumour growth and induced transcriptional reprogramming toward antioxidant defence and ferroptosis resistance. Mechanistically, CEVs activated the KEAP1–NRF2 signalling axis through coordinated downregulation of ACSL4 and KEAP1, leading to enhanced glutathione biosynthesis, increased GPX4 activity, reduced lipid peroxidation and decreased intracellular reactive oxygen species levels. These metabolic alterations suppressed ferroptosis and promoted tumour cell survival. Importantly, pharmacological induction of ferroptosis using RSL3 abolished the tumour-promoting effects of CEVs, demonstrating that ferroptosis suppression is essential for CEVs-mediated tumour progression. Collectively, our findings identify bacterial extracellular vesicles as functional modulators of host redox metabolism and ferroptosis, revealing a previously unrecognized mechanism by which tumour-associated microbiota influence cancer progression.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13160924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147872480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Arsenal of Aromatic Degrading Bacteria: How They Sense, Chase, Adapt and Destroy Environmental Pollutants","authors":"Prashant S. Phale,&nbsp;Tushar Dhamale,&nbsp;Satyajit Subhash","doi":"10.1111/1751-7915.70372","DOIUrl":"10.1111/1751-7915.70372","url":null,"abstract":"<p>Aromatic compounds are ubiquitous arising from natural sources as well as anthropogenic activities posing significant ecological and health risks due to their persistence and toxicity in nature. While bacterial biodegradation of these compounds offers a sustainable strategy, its success usually hinges on integrated phenotypes that are beyond mere catabolic pathways. Phenotype involves multiple processes like sensing pollutants, chemotaxis, transport, membrane adaptation, stress tolerance, regulation at molecular level, and community co-operation. Bacteria sense aromatics via specialized chemoreceptors, triggering metabolism-dependent or independent chemotaxis. Partitioning of hydrophobic pollutants into membranes is countered by membrane modifications and efflux pumps. While facilitated uptake occurs using biosurfactants and specific transporters. Some bacteria exhibit unique carbon-source utilization hierarchies that prioritize aromatics over other carbon sources or co-metabolize, subverting canonical catabolite repression leading to niche dominance. Biofilm formation, cross-feeding and division of labor enhance resilience in bacterial communities. Understanding and integrating these sensing, chemotactic, adaptive and metabolic capabilities are crucial for the rational engineering of bacteria for effective remediation of contaminated sites.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148068736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148067293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Followers' Choice: The Trends Transforming Precision Medicine, Synthetic Biology, and Sustainable Microbiology","authors":"Patricia Bernal,&nbsp;Rocío Palacios-Ferrer,&nbsp;Juan L. Ramos","doi":"10.1111/1751-7915.70370","DOIUrl":"10.1111/1751-7915.70370","url":null,"abstract":"&lt;p&gt;The diffusion of science is evolving rapidly, with social networks playing an increasingly essential role. By analysing the journal's accounts on X (@MicrobialBiote1) and BlueSky (@microbiotech.bsky.social), we identified recent microbiology and biotechnology studies that have captured the interest and engagement of our followers, reflecting the topics that resonate most with our scientific community. Together, these articles reveal emerging trends in precision medicine, microbial ecology, synthetic biology, and sustainable biotechnology.&lt;/p&gt;&lt;p&gt;A central priority in the broader effort to combat diseases is the development of targeted microbial therapies. Choudhury et al. (https://doi.org/10.1111/1751-7915.70241) present an innovative precision antimicrobial strategy against &lt;i&gt;Fusobacterium nucleatum&lt;/i&gt; , a bacterium associated with colorectal cancer. By engineering &lt;i&gt;Lactococcus lactis&lt;/i&gt; to deliver guided antimicrobial peptides (gAMPs), these researchers achieved selective inhibition of the pathogen while preserving the beneficial microbiota. The system demonstrated strong specificity, reduced toxicity, and the ability to maintain microbial diversity in simulated gut environments, emphasizing the potential of engineered probiotics as next-generation therapeutics.&lt;/p&gt;&lt;p&gt;The therapeutic potential of probiotics is further illustrated in a study by Wang et al. on chronic spontaneous urticaria, in which &lt;i&gt;Lactobacillus paragasseri&lt;/i&gt; LG-1 was shown to modulate metabolism, restore microbiota balance, and reduce inflammation through immune pathway regulation (https://doi.org/10.1111/1751-7915.70316). The interest in this area is also reflected in advances in probiotic microencapsulation, which aim to enhance the stability and targeted delivery of beneficial microbes, paving the way for more effective and personalized therapeutic applications (Zhu et al., https://doi.org/10.1111/1751-7915.70305).&lt;/p&gt;&lt;p&gt;Expanding beyond human health, another study by Vidal et al. (https://doi.org/10.1111/1751-7915.70286) explores how Earth's subsurface microbiome can inform the search for extraterrestrial life. Microorganisms thriving in extreme, low-energy environments beneath the Earth's surface provide valuable analogues for potential habitats on Mars and icy moons such as Europa and Enceladus. Their findings suggest that extraterrestrial life may be slow-growing and difficult to detect, reinforcing the need for advanced detection technologies and refined biosignature identification.&lt;/p&gt;&lt;p&gt;Sustainability in agriculture has emerged as a crucial approach to ensuring that food production is balanced with the protection of environmental resources. Microbial volatile organic compounds (VOCs) are highlighted as promising alternatives to chemical pesticides, capable of inhibiting pathogens and promoting plant health (Belt et al., https://doi.org/10.1111/1751-7915.70313). However, translating laboratory findings into field applications remains challenging due to envi","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning Reveals Quantitative Amino Acid Preferences in Bifidobacterium longum Growth","authors":"Hiroki Kaneko,&nbsp;Kana Kadowaki,&nbsp;Shin Yoshimoto,&nbsp;Toshitaka Odamaki,&nbsp;Bei - Wen Ying","doi":"10.1111/1751-7915.70367","DOIUrl":"10.1111/1751-7915.70367","url":null,"abstract":"<p><i>Bifidobacterium longum</i> is a prevalent human gut symbiont whose carbohydrate metabolism is well characterized, whereas the quantitative contribution of amino acids to growth remains unclear. Here, we combined genome-based pathway analysis, growth phenotyping in chemically defined media, and iterative machine-learning-guided medium design to quantify amino acid preferences in <i>B. longum</i> subsp. <i>longum</i> JCM 1217^T. Genome analysis predicted cysteine as the sole auxotrophy, and experiments confirmed that cysteine alone supported growth but did not restore the high maximum cell density and short lag time achieved with a complete amino acid mixture. Regression models and genetic algorithms identified amino acid formulations in the selected optimized compositions that reduced total amino acid input by 66.5% under glucose and 77.2% under lactose while maintaining growth comparable to complete medium. SHAP analysis highlighted tyrosine as the main determinant of maximum cell density, whereas glutamate, leucine, and valine consistently shortened lag time. These results show that amino acid requirements in <i>B. longum</i> extend beyond binary auxotrophy and provide a machine-learning framework for designing streamlined defined media.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 5","pages":""},"PeriodicalIF":5.2,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148067294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}