Microbial Biotechnology最新文献_第10页

Precise Engineering and Efficient Biosynthesis of Robust and High-Activity Human Haemoglobin for Artificial Oxygen Carriers 用于人工氧载体的强韧和高活性人血红蛋白的精确工程和高效生物合成

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-12 DOI: 10.1111/1751-7915.70128

Fan Liu, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du, Xinrui Zhao

引用次数: 0

The Legalome: Microbiology, Omics and Criminal Justice 法律：微生物学，组学和刑事司法

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-12 DOI: 10.1111/1751-7915.70129

Alan C. Logan, Pragya Mishra, Susan L. Prescott

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引用次数: 0

Precise Engineering and Efficient Biosynthesis of Robust and High-Activity Human Haemoglobin for Artificial Oxygen Carriers 用于人工氧载体的强韧和高活性人血红蛋白的精确工程和高效生物合成

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-12 DOI: 10.1111/1751-7915.70128

Fan Liu, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du, Xinrui Zhao

引用次数: 0

The Legalome: Microbiology, Omics and Criminal Justice 法律：微生物学，组学和刑事司法

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-12 DOI: 10.1111/1751-7915.70129

Alan C. Logan, Pragya Mishra, Susan L. Prescott

{"title":"The Legalome: Microbiology, Omics and Criminal Justice","authors":"Alan C. Logan, Pragya Mishra, Susan L. Prescott","doi":"10.1111/1751-7915.70129","DOIUrl":"https://doi.org/10.1111/1751-7915.70129","url":null,"abstract":"Advances in neuromicrobiology and related omics technologies have reinforced the idea that unseen microbes play critical roles in human cognition and behaviour. Included in this research is evidence indicating that gut microbes, through direct and indirect pathways, can influence aggression, anger, irritability and antisocial behaviour. Moreover, gut microbes can manufacture chemicals that are known to compromise cognition. For example, recent court decisions in the United States and Europe acknowledge that gut microbes can produce high levels of ethanol, without consumption of alcohol by the defendants. The dismissal of driving while intoxicated charges in these cases—so-called auto-brewery syndrome—highlights the way in which microbiome knowledge will enhance the precision, objectivity and fairness of our legal systems. Here in this opinion essay, we introduce the concept of the ‘legalome’—the application of microbiome and omics science to forensic psychiatry and criminal law. We argue that the rapid pace of microbial discoveries, including those that challenge ideas of free will and moral responsibility, will necessitate a reconsideration of traditional legal doctrines and justifications of retributive punishment. The implications extend beyond the courtroom, challenging us to reconsider how environmental factors—from diet to socioeconomic conditions—might shape preventative and rehabilitative efforts through their effects on the microbiome.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Permeabilisation of the Outer Membrane of Escherichia coli for Enhanced Transport of Complex Molecules 大肠杆菌外膜的通透性增强了复杂分子的运输

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-09 DOI: 10.1111/1751-7915.70122

Ivan Casas-Rodrigo, Tobias Vornholt, Kathrin Castiglione, Tania Michelle Roberts, Markus Jeschek, Thomas R. Ward, Sven Panke

{"title":"Permeabilisation of the Outer Membrane of Escherichia coli for Enhanced Transport of Complex Molecules","authors":"Ivan Casas-Rodrigo, Tobias Vornholt, Kathrin Castiglione, Tania Michelle Roberts, Markus Jeschek, Thomas R. Ward, Sven Panke","doi":"10.1111/1751-7915.70122","DOIUrl":"https://doi.org/10.1111/1751-7915.70122","url":null,"abstract":"The bacterial envelope plays a critical role in maintaining essential cellular functions by selectively regulating import and export. The selectivity of this envelope can restrict the utilisation of externally provided compounds, thereby restricting the functional space of cellular engineering. This study systematically investigates the potential of large pore outer membrane proteins (OMPs) to enhance outer membrane permeability for diverse challenging compounds. We focus on the general porin OmpF, which facilitates the diffusion of water and small molecules, and specific OMP transporters FhuA and FepA, which mediate the translocation of small hydrophilic compounds. Through comprehensive characterisation, we evaluate the effects of recombinant expression of OMPs and engineered variants for small and hydrophilic compounds, aromatic molecules and bulky molecules and apply our findings to address two critical contemporary transport challenges: the uptake of large metal-containing cofactors for artificial metalloenzymes and non-permeant fluorescent Halo-ligands for in vivo protein labelling. Notably, we demonstrate significant improvements in ArM-catalysis and labelling. This study provides a practical guide for designing experiments that include outer-membrane-transport-limiting steps. This study highlights the potential of engineered OMPs to overcome the limitations imposed by the cell envelope, enabling the incorporation of complex molecules and expanding the frontiers of cellular engineering.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Permeabilisation of the Outer Membrane of Escherichia coli for Enhanced Transport of Complex Molecules 大肠杆菌外膜的通透性增强了复杂分子的运输

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-09 DOI: 10.1111/1751-7915.70122

Ivan Casas-Rodrigo, Tobias Vornholt, Kathrin Castiglione, Tania Michelle Roberts, Markus Jeschek, Thomas R. Ward, Sven Panke

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引用次数: 0

Characterisation of the Self-Sufficient Cytochrome P450 CYP116B234 From Rhodococcus globerulus and Its Suggested Native Role in 2-Hydroxyphenylacetic Acid Metabolism 球状红球菌自给细胞色素P450 CYP116B234的特性及其在2-羟基苯乙酸代谢中的天然作用

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-08 DOI: 10.1111/1751-7915.70125

Simran Kundral, Hannah Beamish, Peter D. Giang, Lauren J. Salisbury, Amanda S. Nouwens, Sunil K. Khare, Paul V. Bernhardt, Jeffrey R. Harmer, Stephen G. Bell, James J. De Voss

{"title":"Characterisation of the Self-Sufficient Cytochrome P450 CYP116B234 From Rhodococcus globerulus and Its Suggested Native Role in 2-Hydroxyphenylacetic Acid Metabolism","authors":"Simran Kundral, Hannah Beamish, Peter D. Giang, Lauren J. Salisbury, Amanda S. Nouwens, Sunil K. Khare, Paul V. Bernhardt, Jeffrey R. Harmer, Stephen G. Bell, James J. De Voss","doi":"10.1111/1751-7915.70125","DOIUrl":"https://doi.org/10.1111/1751-7915.70125","url":null,"abstract":"Cytochromes P450 (P450s) are exceptional biocatalysts that enable the selective oxidation of unactivated C–H bonds using molecular oxygen. Typically, auxiliary redox partner proteins deliver electrons from NAD(P)H to the P450, enabling oxygen activation. However, associating native redox partners with P450s can be challenging, particularly when they are genomically separated. Self-sufficient P450s, where the P450 heme domain is naturally fused to redox partners, represent a simpler, single-protein system. Here, we present CYP116B234, a novel self-sufficient P450 from Rhodococcus globerulus, comprising fused heme and phthalate-family oxygenase reductase (PFOR) domains. The gene encoding CYP116B234 was codon-optimised for heterologous expression in E. coli and subsequently purified to homogeneity. Spectroelectrochemical analysis and electron paramagnetic resonance spectroscopy were performed to determine the redox potentials of the heme and associated Fe–S and FMN cofactors of the PFOR domain. CYP116B234 binds and efficiently oxidises the substituted aromatic compound 2-hydroxyphenylacetic acid (2-HPA) to homogentisic acid. Quantitative proteomics revealed the expression of CYP116B234 in R. globerulus grown on 2-HPA, suggesting a role in initiating 2-HPA degradation. This study presents a new addition to the self-sufficient CYP116 family and provides evidence for their native function.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of the Self-Sufficient Cytochrome P450 CYP116B234 From Rhodococcus globerulus and Its Suggested Native Role in 2-Hydroxyphenylacetic Acid Metabolism 球状红球菌自给细胞色素P450 CYP116B234的特性及其在2-羟基苯乙酸代谢中的天然作用

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-08 DOI: 10.1111/1751-7915.70125

Simran Kundral, Hannah Beamish, Peter D. Giang, Lauren J. Salisbury, Amanda S. Nouwens, Sunil K. Khare, Paul V. Bernhardt, Jeffrey R. Harmer, Stephen G. Bell, James J. De Voss

{"title":"Characterisation of the Self-Sufficient Cytochrome P450 CYP116B234 From Rhodococcus globerulus and Its Suggested Native Role in 2-Hydroxyphenylacetic Acid Metabolism","authors":"Simran Kundral, Hannah Beamish, Peter D. Giang, Lauren J. Salisbury, Amanda S. Nouwens, Sunil K. Khare, Paul V. Bernhardt, Jeffrey R. Harmer, Stephen G. Bell, James J. De Voss","doi":"10.1111/1751-7915.70125","DOIUrl":"https://doi.org/10.1111/1751-7915.70125","url":null,"abstract":"Cytochromes P450 (P450s) are exceptional biocatalysts that enable the selective oxidation of unactivated C–H bonds using molecular oxygen. Typically, auxiliary redox partner proteins deliver electrons from NAD(P)H to the P450, enabling oxygen activation. However, associating native redox partners with P450s can be challenging, particularly when they are genomically separated. Self-sufficient P450s, where the P450 heme domain is naturally fused to redox partners, represent a simpler, single-protein system. Here, we present CYP116B234, a novel self-sufficient P450 from Rhodococcus globerulus, comprising fused heme and phthalate-family oxygenase reductase (PFOR) domains. The gene encoding CYP116B234 was codon-optimised for heterologous expression in E. coli and subsequently purified to homogeneity. Spectroelectrochemical analysis and electron paramagnetic resonance spectroscopy were performed to determine the redox potentials of the heme and associated Fe–S and FMN cofactors of the PFOR domain. CYP116B234 binds and efficiently oxidises the substituted aromatic compound 2-hydroxyphenylacetic acid (2-HPA) to homogentisic acid. Quantitative proteomics revealed the expression of CYP116B234 in R. globerulus grown on 2-HPA, suggesting a role in initiating 2-HPA degradation. This study presents a new addition to the self-sufficient CYP116 family and provides evidence for their native function.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep-Learning-Based Approaches for Rational Design of Stapled Peptides With High Antimicrobial Activity and Stability 基于深度学习的高效抗菌肽合理设计方法

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-05 DOI: 10.1111/1751-7915.70121

Ruole Chen, Yuhao You, Yanchao Liu, Xin Sun, Tianyue Ma, Xingzhen Lao, Heng Zheng

引用次数: 0

Engineering Genome-Free Bacterial Cells for Effective SARS-COV-2 Neutralisation 有效中和SARS-COV-2的工程无基因组细菌细胞

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-05 DOI: 10.1111/1751-7915.70109

Yutong Yin, Chang Liu, Xianglin Ji, Yun Wang, Juthathip Mongkolsapaya, Gavin R. Screaton, Zhanfeng Cui, Wei E. Huang

{"title":"Engineering Genome-Free Bacterial Cells for Effective SARS-COV-2 Neutralisation","authors":"Yutong Yin, Chang Liu, Xianglin Ji, Yun Wang, Juthathip Mongkolsapaya, Gavin R. Screaton, Zhanfeng Cui, Wei E. Huang","doi":"10.1111/1751-7915.70109","DOIUrl":"https://doi.org/10.1111/1751-7915.70109","url":null,"abstract":"The COVID-19 pandemic has caused unparalleled impacts on global social dynamics, healthcare systems and economies, highlighting the urgent need for effective interventions to address current challenges and future pandemic preparedness. This study introduces a novel virus neutralisation platform based on SimCells (~1 μm) and mini-SimCells (100–200 nm), which are chromosome-free and non-replicating bacteria from an LPS-free Escherichia coli strain (ClearColi). SimCells and mini-SimCells were engineered to display nanobodies on their surface, specifically targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein – a critical immunogenic fragment essential for viral entry into host cells. It was demonstrated that nanobody-expressing SimCells achieved over 90% blocking efficiency against synthesised RBD from both the original Wuhan and the B.1.351 (Beta) variant using competitive enzyme-linked immunosorbent assay (ELISA) assay. More importantly, live virus neutralisation assays demonstrated that NB6 nanobody-presenting mini-SimCells effectively neutralised the live SARS-CoV-2 Victoria variant with an IC50 of 2.95 × 109 ± 1.40 × 108 mini-SimCells/mL. Similarly, VE nanobody-presenting mini-SimCells effectively neutralised the B.1.351 (Beta) variant of the SARS-CoV-2 virus with an IC50 of 5.68 × 109 ± 9.94 × 108 mini-SimCells/mL. The mini-SimCells successfully protected Vero cells, a cell line derived from the kidney of an African green monkey, from infection by the live virus of SARS-CoV-2 and its variants. These results suggest that SimCell-based neutralisation offers a promising strategy for the prevention and treatment of SARS-CoV-2, and potentially other viral infections.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0