Microbial Biotechnology最新文献_第9页

Genetic Bioaugmentation-Mediated Bioremediation of Terephthalate in Soil Microcosms Using an Engineered Environmental Plasmid 利用工程化环境质粒在土壤微生态系统中对对苯二甲酸盐进行基因生物增殖介导的生物修复。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-01-13 DOI: 10.1111/1751-7915.70071

Alejandro Marquiegui – Alvaro, Anastasia Kottara, Micaela Chacón, Lisa Cliffe, Michael Brockhurst, Neil Dixon

{"title":"Genetic Bioaugmentation-Mediated Bioremediation of Terephthalate in Soil Microcosms Using an Engineered Environmental Plasmid","authors":"Alejandro Marquiegui – Alvaro, Anastasia Kottara, Micaela Chacón, Lisa Cliffe, Michael Brockhurst, Neil Dixon","doi":"10.1111/1751-7915.70071","DOIUrl":"10.1111/1751-7915.70071","url":null,"abstract":"Harnessing in situ microbial communities to clean-up polluted natural environments is a potentially efficient means of bioremediation, but often the necessary genes to breakdown pollutants are missing. Genetic bioaugmentation, whereby the required genes are delivered to resident bacteria via horizontal gene transfer, offers a promising solution to this problem. Here, we engineered a conjugative plasmid previously isolated from soil, pQBR57, to carry a synthetic set of genes allowing bacteria to consume terephthalate, a chemical component of plastics commonly released during their manufacture and breakdown. Our engineered plasmid caused a low fitness cost and was stably maintained in terephthalate-contaminated soil by the bacterium P. putida. Plasmid carriers efficiently bioremediated contaminated soil in model soil microcosms, achieving complete breakdown of 3.2 mg/g of terephthalate within 8 days. The engineered plasmid horizontally transferred the synthetic operon to P. fluorescens in situ, and the resulting transconjugants degraded 10 mM terephthalate during a 180-h incubation. Our findings show that environmental plasmids carrying synthetic catabolic operons can be useful tools for in situ engineering of microbial communities to perform clean-up even of complex environments like soil.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Isolation to Application: Utilising Phage-Antibiotic Synergy in Murine Bacteremia Model to Combat Multidrug-Resistant Enterococcus faecalis 从分离到应用：在小鼠菌血症模型中利用噬菌体与抗生素的协同作用来对抗耐多药粪肠球菌。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-01-12 DOI: 10.1111/1751-7915.70075

Fatma Al-zahraa A. Yehia, Galal Yahya, Eslam M. Elsayed, Javier Serrania, Anke Becker, Salwa E. Gomaa

{"title":"From Isolation to Application: Utilising Phage-Antibiotic Synergy in Murine Bacteremia Model to Combat Multidrug-Resistant Enterococcus faecalis","authors":"Fatma Al-zahraa A. Yehia, Galal Yahya, Eslam M. Elsayed, Javier Serrania, Anke Becker, Salwa E. Gomaa","doi":"10.1111/1751-7915.70075","DOIUrl":"10.1111/1751-7915.70075","url":null,"abstract":"<div>\u0000 \u0000 Enterococcus species, natural inhabitants of the human gut, have become major causes of life-threatening bloodstream infections (BSIs) and the third most frequent cause of hospital-acquired bacteremia. The rise of high-level gentamicin resistance (HLGR) in enterococcal isolates complicates treatment and revives bacteriophage therapy. This study isolated and identified forty E. faecalis clinical isolates, with 30% exhibiting HLGR. The HLGR5 isolate, resistant to fosfomycin, vancomycin, and linezolid, was used to isolate the vB_EfaS_SZ1 phage from effluent water. This phage specifically lysed 42% of HLGR isolates. vB_EfaS_SZ1 demonstrated beneficial traits, including thermal stability, acid–base tolerance, a short latent period, and a large burst size. The phage genome comprises a 40,942 bp linear double-stranded DNA with 65 open reading frames (ORFs). The genome closely resembled Enterococcus phages, classifying it within the Efquatrovirus genus. Phage-antibiotic synergy was assessed using checkerboard assays and time-killing analyses, revealing enhanced bacteriolytic activity of ampicillin and fosfomycin, with significant reductions in minimum inhibitory concentration values. In a mouse bacteremia model, phage-antibiotic combinations significantly reduced E. faecalis liver burden compared to monotherapies. Histopathological analysis confirmed therapeutic synergy, showing reduced inflammation and improved hepatocyte regeneration. These findings underscore the potential of phage vB_EfaS_SZ1 as an adjunct to antibiotic therapy for resistant enterococcal bacteremia.\u0000 </div>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Immunisation With Non-GMO Surface Displayed SARS-CoV-2 Spike Epitopes on Bacteria-Like Particles Provokes Robust Humoral and Cellular Immune Responses, and Modulated the Gut Microbiome in Mice 在细菌样颗粒上显示非转基因表面的SARS-CoV-2刺突表位的口服免疫可激发强大的体液和细胞免疫反应，并调节小鼠肠道微生物群。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-01-11 DOI: 10.1111/1751-7915.70073

Robie Vasquez, Ji Hoon Song, Remilyn M. Mendoza, In-Chan Hwang, Bernadette B. Bagon, Lars Engstrand, Valerie Diane Valeriano, Dae-Kyung Kang

{"title":"Oral Immunisation With Non-GMO Surface Displayed SARS-CoV-2 Spike Epitopes on Bacteria-Like Particles Provokes Robust Humoral and Cellular Immune Responses, and Modulated the Gut Microbiome in Mice","authors":"Robie Vasquez, Ji Hoon Song, Remilyn M. Mendoza, In-Chan Hwang, Bernadette B. Bagon, Lars Engstrand, Valerie Diane Valeriano, Dae-Kyung Kang","doi":"10.1111/1751-7915.70073","DOIUrl":"10.1111/1751-7915.70073","url":null,"abstract":"The coronavirus disease 2019 (COVID-19) is a fatal disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). To date, several vaccines have been developed to combat the spread of this virus. Mucosal vaccines using food-grade bacteria, such as Lactobacillus spp., are promising strategies for developing safe and effective vaccines against SARS-CoV-2. In this study, we designed a non-GMO surface-displayed SARS-CoV-2 spike S1 epitope on Limosilactobacillus fermentum-derived bacteria-like particles (BLPs). After that, we evaluated its efficacy to induce immune responses in immunocompetent mice. Moreover, we examined the influence of oral immunisation on the gut microbiome and microbiota metabolites. Twenty-eight 6-week-old male C57BL/6 mice were orally immunised with the following: PBS (control), Lm. fermentum-derived BLPs only, BLPs displaying SARS-CoV-2 spike S1-2, or BLPs displaying SARS-CoV-2 spike S1-3 epitopes. Our results showed that mucosal immunisation of mice with surface-displayed SARS-CoV-2 spike epitopes provoked high-level secretory IgA and systemic IgG production. Moreover, the immunisation exhibited a Th1-like immune response, characterised by an elevated IgG2a-to-IgG1 ratio and high antiviral IFN-γ production. In addition, we observed gut microbiome modulation and increased butyrate production in immunised mice. Overall, the use of Lm. fermentum-derived BLPs and the anchor CshA to display SARS-CoV-2 spike S1epitopes is a promising novel strategy in developing a cost-effective, non-GMO mucosal vaccine alternative against SARS-CoV-2.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11724470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI Methods for Antimicrobial Peptides: Progress and Challenges 抗菌肽的人工智能方法：进展与挑战。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-01-04 DOI: 10.1111/1751-7915.70072

Carlos A. Brizuela, Gary Liu, Jonathan M. Stokes, Cesar de la Fuente-Nunez

{"title":"AI Methods for Antimicrobial Peptides: Progress and Challenges","authors":"Carlos A. Brizuela, Gary Liu, Jonathan M. Stokes, Cesar de la Fuente-Nunez","doi":"10.1111/1751-7915.70072","DOIUrl":"10.1111/1751-7915.70072","url":null,"abstract":"Antimicrobial peptides (AMPs) are promising candidates to combat multidrug-resistant pathogens. However, the high cost of extensive wet-lab screening has made AI methods for identifying and designing AMPs increasingly important, with machine learning (ML) techniques playing a crucial role. AI approaches have recently revolutionised this field by accelerating the discovery of new peptides with anti-infective activity, particularly in preclinical mouse models. Initially, classical ML approaches dominated the field, but recently there has been a shift towards deep learning (DL) models. Despite significant contributions, existing reviews have not thoroughly explored the potential of large language models (LLMs), graph neural networks (GNNs) and structure-guided AMP discovery and design. This review aims to fill that gap by providing a comprehensive overview of the latest advancements, challenges and opportunities in using AI methods, with a particular emphasis on LLMs, GNNs and structure-guided design. We discuss the limitations of current approaches and highlight the most relevant topics to address in the coming years for AMP discovery and design.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic Plasticity During Organofluorine Degradation Revealed by Adaptive Evolution 适应进化揭示有机氟降解过程中的表型可塑性。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-12-26 DOI: 10.1111/1751-7915.70066

Madeline R. O'Connor, Calvin J. Thoma, Anthony G. Dodge, Lawrence P. Wackett

{"title":"Phenotypic Plasticity During Organofluorine Degradation Revealed by Adaptive Evolution","authors":"Madeline R. O'Connor, Calvin J. Thoma, Anthony G. Dodge, Lawrence P. Wackett","doi":"10.1111/1751-7915.70066","DOIUrl":"10.1111/1751-7915.70066","url":null,"abstract":"A major factor limiting the biodegradation of organofluorine compounds has been highlighted as fluoride anion toxicity produced by defluorinating enzymes. Here, two highly active defluorinases with different activities were constitutively expressed in Pseudomonas putida ATCC 12633 to examine adaption to fluoride stress. Each strain was grown on α-fluorophenylacetic acid as the sole carbon source via defluorination to mandelic acid, and each showed immediate fluoride release and delayed growth. Adaptive evolution was performed for each recombinant strain by serial transfer. Both strains adapted to show a much shorter lag and a higher growth yield. The observed adaptation occurred rapidly and reproducibly, within 50 generations each time. After adaption, growth with 50–70 mM α-fluorophenylacetic acid was significantly faster with more fluoride release than a preadapted culture due to larger cell populations. Genomic sequencing of both pre- and postadapted strain pairs revealed decreases in the defluorinase gene content. With both defluorinases, adaption produced a 56%–57% decrease in the plasmid copy number. Additionally, during adaption of the strain expressing the faster defluorinase, two plasmids were present: the original and a derivative in which the defluorinase gene was deleted. An examination of the enzyme rates in the pathway suggested that the defluorinase rate was concurrently optimised for pathway flux and minimising fluoride toxicity. The rapid alteration of plasmid copy number and mutation was consistent with other studies on microbial responses to stresses such as antibiotics. The data presented here support the idea that fluoride stress is significant during the biodegradation of organofluorine compounds and suggest engineered strains will be under strong selective pressure to decrease fluoride stress.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 12","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11670473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus subtilis Intraspecies Interactions Shape Probiotic Activity Against Salmonella Typhimurium 枯草芽孢杆菌种内相互作用形成对抗鼠伤寒沙门氏菌的益生菌活性。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-12-24 DOI: 10.1111/1751-7915.70065

Eva Kovačec, Barbara Kraigher, Eli Podnar, Bram Lories, Hans Steenackers, Ines Mandic-Mulec

{"title":"Bacillus subtilis Intraspecies Interactions Shape Probiotic Activity Against Salmonella Typhimurium","authors":"Eva Kovačec, Barbara Kraigher, Eli Podnar, Bram Lories, Hans Steenackers, Ines Mandic-Mulec","doi":"10.1111/1751-7915.70065","DOIUrl":"10.1111/1751-7915.70065","url":null,"abstract":"Commercial probiotics are often formulated as multi-strain cocktails, but the effects of social interactions, particularly between strains of a species, are often neglected, despite their potential to contribute to higher-order interactions where these interactions could affect those with a third party. In this study, we investigated the probiotic potential of a collection of Bacillus subtilis strains against Salmonella Typhimurium in single-strain and mixed cultures. The results indicate a promising probiotic potential of B. subtilis as 38 out of 39 strains significantly inhibited the growth of S. Typhimurium. Next, we tested the effect of mixing B. subtilis strains that differ in their inhibitory potency against S. Typhimurium. The results show that strong inhibition by one strain can be significantly reduced by mixing with a less effective strain. Moreover, mixing similarly effective strains mostly resulted in a decreased growth inhibition of the pathogen. Additionally, we found a group of highly aggressive strains, which completely eliminated other B. subtilis strains in the two-strain mixtures. Overall, this work shows that intraspecies interactions between B. subtilis strains can significantly alter the probiotic effect against S. Typhimurium, which is of great importance for future research on the development of multi-strain probiotics.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 12","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Easy-Curing and pH-Regulated CRISPR-Cas9 Plasmids for Gene Editing and Plasmid Curing in Lactococcus cremoris 用于乳球菌基因编辑和质粒固化的易固化和 pH 值调节 CRISPR-Cas9 质粒

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-12-20 DOI: 10.1111/1751-7915.70060

Javier Nicolás Garay-Novillo, José Ángel Ruiz-Masó, Gloria del Solar, José Luis Barra

{"title":"Easy-Curing and pH-Regulated CRISPR-Cas9 Plasmids for Gene Editing and Plasmid Curing in Lactococcus cremoris","authors":"Javier Nicolás Garay-Novillo, José Ángel Ruiz-Masó, Gloria del Solar, José Luis Barra","doi":"10.1111/1751-7915.70060","DOIUrl":"https://doi.org/10.1111/1751-7915.70060","url":null,"abstract":"In this work, we developed a plasmid-based CRISPR-Cas9 strategy for editing Lactococcus cremoris, which allows easy generation of plasmid-free strains with the desired modification. We constructed versatile shuttle vectors based on the theta-type pAMβ1 promiscuous replicon and p15A ori, expressing both the Cas9 nuclease gene (under pH-regulated promoters derived from P170) and a single-guide RNA for specific targeting (under a strong constitutive promoter). The vectors designed for plasmid targeting were very effective for low- and high-copy-number plasmid curing in L. cremoris, and their targeting efficiency was shown to be tunable by regulating cas9 expression. For chromosome editing, we implemented a host-independent method that enhances double-homologous recombination events using plasmids expressing the genes encoding λRed-phage Redβ recombinase and Escherichia coli single-stranded DNA binding protein (EcSSB). By coupling either the endogenous recombination machinery or the Redβ-EcSSB-assisted recombination system with our novel chromosome-targeting CRISPR-Cas9 plasmids, we efficiently generated and selected thousands of gene-edited cells. Examination of the impact of the constructed CRISPR-Cas9 vectors on host fitness revealed no Cas9-associated toxicity, and, remarkably, these vectors exhibited a very high loss rate when growing the bacterial host cells in the absence of selective pressure.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 12","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142868877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Easy-Curing and pH-Regulated CRISPR-Cas9 Plasmids for Gene Editing and Plasmid Curing in Lactococcus cremoris cremoris乳球菌基因编辑和质粒固化的易固化和ph调节CRISPR-Cas9质粒

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-12-20 DOI: 10.1111/1751-7915.70060

Javier Nicolás Garay-Novillo, José Ángel Ruiz-Masó, Gloria del Solar, José Luis Barra

{"title":"Easy-Curing and pH-Regulated CRISPR-Cas9 Plasmids for Gene Editing and Plasmid Curing in Lactococcus cremoris","authors":"Javier Nicolás Garay-Novillo, José Ángel Ruiz-Masó, Gloria del Solar, José Luis Barra","doi":"10.1111/1751-7915.70060","DOIUrl":"https://doi.org/10.1111/1751-7915.70060","url":null,"abstract":"In this work, we developed a plasmid-based CRISPR-Cas9 strategy for editing Lactococcus cremoris, which allows easy generation of plasmid-free strains with the desired modification. We constructed versatile shuttle vectors based on the theta-type pAMβ1 promiscuous replicon and p15A ori, expressing both the Cas9 nuclease gene (under pH-regulated promoters derived from P170) and a single-guide RNA for specific targeting (under a strong constitutive promoter). The vectors designed for plasmid targeting were very effective for low- and high-copy-number plasmid curing in L. cremoris, and their targeting efficiency was shown to be tunable by regulating cas9 expression. For chromosome editing, we implemented a host-independent method that enhances double-homologous recombination events using plasmids expressing the genes encoding λRed-phage Redβ recombinase and Escherichia coli single-stranded DNA binding protein (EcSSB). By coupling either the endogenous recombination machinery or the Redβ-EcSSB-assisted recombination system with our novel chromosome-targeting CRISPR-Cas9 plasmids, we efficiently generated and selected thousands of gene-edited cells. Examination of the impact of the constructed CRISPR-Cas9 vectors on host fitness revealed no Cas9-associated toxicity, and, remarkably, these vectors exhibited a very high loss rate when growing the bacterial host cells in the absence of selective pressure.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 12","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142868929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Penitentiaries: Bringing microbiological literacy to the fringes of society 监狱：将微生物知识带到社会边缘。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-12-19 DOI: 10.1111/1751-7915.70052

Víctor de Lorenzo

{"title":"Penitentiaries: Bringing microbiological literacy to the fringes of society","authors":"Víctor de Lorenzo","doi":"10.1111/1751-7915.70052","DOIUrl":"10.1111/1751-7915.70052","url":null,"abstract":"This report highlights a science outreach effort for prisons launched by the Spanish National Research Council (CSIC) in collaboration with the NGO Solidarios para el Desarrollo. The Microbiology-focused part of the initiative aims at educating inmates on some basic facts, in order to raise awareness about microorganisms and their impact on daily life. The outline of the talks, inspired by the International Initiative for Microbial Literacy, aims to encourage this collective to move from passive listeners into active participants, helping them understand that Earth is a microbial planet, and that their bodies harbour vast microbiomes that affect their health and social interactions. The talks introduce Microbiology using simple metaphors and emphasize the role of beneficial microorganisms. By explaining the power of microscopes, inmates are shown the hidden microbial world that surrounds them, sparking interest and curiosity. The talks also cover microbial biotechnology, using examples such as bioplastics, anti-cavity bacteria, and skin microorganisms designed for acne prevention. Overall, this outreach initiative seeks to provide inmates with valuable scientific knowledge, fostering curiosity and critical thinking. Despite the challenges of delivering such content in a prison setting, the initiative demonstrates that even marginalized groups can benefit from microbiological literacy, helping them to both endure their terms and eventually reintegrate into society.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 12","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Desert-adapted plant growth-promoting pseudomonads modulate plant auxin homeostasis and mitigate salinity stress 荒漠植物促生长假单胞菌调节植物生长素稳态，减轻盐胁迫。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-12-18 DOI: 10.1111/1751-7915.70043

Ramona Marasco, Maria J. Mosqueira, Kholoud A. Seferji, Sarah M. Al Romaih, Grégoire Michoud, Jian Xu, Cristina Bez, Tatiana Castillo Hernandez, Vittorio Venturi, Ikram Blilou, Daniele Daffonchio

{"title":"Desert-adapted plant growth-promoting pseudomonads modulate plant auxin homeostasis and mitigate salinity stress","authors":"Ramona Marasco, Maria J. Mosqueira, Kholoud A. Seferji, Sarah M. Al Romaih, Grégoire Michoud, Jian Xu, Cristina Bez, Tatiana Castillo Hernandez, Vittorio Venturi, Ikram Blilou, Daniele Daffonchio","doi":"10.1111/1751-7915.70043","DOIUrl":"10.1111/1751-7915.70043","url":null,"abstract":"By providing adaptive advantages to plants, desert microorganisms are emerging as promising solutions to mitigate the negative and abrupt effects of climate change in agriculture. Among these, pseudomonads, commonly found in soil and in association with plants' root system, have been shown to enhance plant tolerance to salinity and drought, primarily affecting root system architecture in various hosts. However, a comprehensive understanding of how these bacteria affect plant responses at the cellular, physiological and molecular levels is still lacking. In this study, we investigated the effects of two Pseudomonas spp. strains, E102 and E141, which were previously isolated from date palm roots and have demonstrated efficacy in promoting drought tolerance in their hosts. These strains colonize plant roots, influencing root architecture by inhibiting primary root growth while promoting root hair elongation and lateral root formation. Strains E102 and E141 increased auxin levels in Arabidopsis, whereas this effect was diminished in IAA-defective mutant strains, which exhibited reduced IAA production. In all cases, the effectiveness of the bacteria relies on the functioning of the plant auxin response and transport machinery. Notably, such physiological and morphological changes provide an adaptive advantage to the plant, specifically under stress conditions such as salinity. Collectively, this study demonstrates that by leveraging the host's auxin signalling machinery, strains E102 and E141 significantly improve plant resilience to abiotic stresses, positioning them as potential biopromoters/bioprotectors for crop production and ecosystem restoration in alignment with Nature-based Solution approaches.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 12","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0