Microbial Biotechnology最新文献

{"title":"Phage-Antibiotic Combinations for Pseudomonas: Successes in the Clinic and In Vitro Tenuously Connected","authors":"Rabia Fatima,&nbsp;Alexander P. Hynes","doi":"10.1111/1751-7915.70193","DOIUrl":"10.1111/1751-7915.70193","url":null,"abstract":"<p>Antimicrobial resistance challenges researchers to innovate strategies to enhance the effectiveness of our existing antibiotics. Bacteriophage (phage, bacterial virus)-antibiotic combinations present a promising synergistic approach, particularly for drug-resistant infections such as those caused by Pseudomonas aeruginosa. This approach offers many advantages: enhanced bacterial killing (both planktonic and biofilm), eliminating persister cells, re-sensitization to drugs, and inhibiting resistance spread by targeting plasmids encoding resistant genes. Interestingly, even phages traditionally excluded from therapy – those capable of entering dormancy in the bacterial host – exhibit unique, potent synergy with antibiotics. Despite these clear in vitro benefits and the comparatively strong performance of phage antibiotic combinations in the clinic, translating in vitro efficacy to patient outcomes remain challenging. The lack of standardized metrics for measuring phage-antibiotic interaction complicates cross-study comparisons. In many instances, it is also difficult to translate these in vitro findings to clinically relevant metrics – for example, increased progeny size in vitro is unlikely to contribute meaningfully to treatment success. Addressing these gaps will allow us to fully harness the potential of phage-antibiotic combinations and bridge the disconnect between in vitro results and clinical success.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How Can ‘Health in All Policies’ Help Maximise the Potential of Microbial Biotechnologies for Health, Equity and Sustainability?","authors":"Margaret J. Douglas,&nbsp;Liz Green,&nbsp;James Timmis,&nbsp;Timo Clemens,&nbsp;Kenneth Timmis","doi":"10.1111/1751-7915.70194","DOIUrl":"10.1111/1751-7915.70194","url":null,"abstract":"<p>Microbial biotechnologies could affect health through multiple pathways, including impacts on food, nutrition, and the physical, economic, and social environment. Health in All Policies is an approach to ensure that plans and policies in all sectors maximise health gains and minimise any health risks. This approach often uses health impact assessment as a structured process to identify and assess positive and negative health impacts and make recommendations to improve these. There are very few examples where HIA has been applied to the implementation of microbial biotechnologies. As more biotechnologies are developed and implemented, more routine use of HIA could help to avoid adverse effects and realise their potential to improve health and reduce health inequalities. This will require greater awareness and understanding of the breadth of links to health, research to build the evidence base for these links, and governance mechanisms to oversee the development and implementation of microbial biotechnologies that prioritise health, equity and sustainability.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70194","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Metallo-β-Lactamase AMM-1 From Alteromonas mangrovi Reveals a Cryptic Environmental Reservoir of Carbapenem Resistance","authors":"Xuan Wu,&nbsp;Xinjing Han,&nbsp;Lu Zhu,&nbsp;Ningning Pi,&nbsp;Yi Li,&nbsp;Rong Xiang","doi":"10.1111/1751-7915.70191","DOIUrl":"10.1111/1751-7915.70191","url":null,"abstract":"<p>Carbapenem resistance driven by metallo-β-lactamases (MBLs) poses a formidable global challenge as these enzymes can degrade a wide range of β-lactam antibiotics, including last-line carbapenems. Despite extensive documentation of MBL-producing pathogens, their evolutionary origins and ecological reservoirs are still poorly understood. Here, we report the discovery and in-depth characterisation of AMM-1, a previously unrecognised B1.2 MBL identified within a metagenome-assembled genome of <i>Alteromonas mangrovi</i> obtained from the Yangtze River Estuary. Comparative sequence analyses and phylogenetics reveal that AMM-1 clusters closely with clinically significant MBLs, underscoring its potential impact to human health. Structural modelling confirms the presence of a conserved di-zinc binding site critical for β-lactam hydrolysis, while heterologous expression in <i>Escherichia coli</i> (<i>E. coli</i>) demonstrates a marked increase in resistance against multiple β-lactam classes, including carbapenems. Phylogenetic depth analysis and ancestral reconstruction delineate AMM-1's distinct evolutionary path, placing it deeper than IMP-1 and SPM-1 but shallower than NDM-1. Flexibility simulations reveal unique active-site loop dynamics (L3 and L10), with reduced mobility in key regions that shape substrate binding stability and spectrum. Notably, AMM-1 is stably located on the host chromosome without flanking mobile genetic elements, suggesting that it may have persisted as a vertically inherited trait rather than a recently acquired component of a mobile resistome. These findings highlight the capacity of environmental microbes to serve as long-standing, cryptic reservoirs of potent resistance determinants, emphasising the need for integrated environmental surveillance and preemptive stewardship strategies. By unveiling the molecular and functional properties of AMM-1, this work provides critical insights into how resistance elements can reside, evolve and potentially mobilise within natural habitats, ultimately informing efforts to predict and mitigate the future emergence of carbapenem-resistant bacterial pathogens.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70191","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Bat to Worse: The Pivotal Role of Bats for Viral Zoonosis","authors":"Harald Brüssow","doi":"10.1111/1751-7915.70190","DOIUrl":"10.1111/1751-7915.70190","url":null,"abstract":"<p>Zoonotic infections are increasingly observed and bats (Chiroptera) are playing a pivotal role here. The causal chain of events has been elucidated for Henipavirus (family: paramyxoviruses) infections. Deforestation combined with climate change has reduced the food sources of Pteropus fruit bats and attracted them to fruit trees planted around piggeries in Malaysia, transmitting Nipah virus to pigs as amplifying hosts and then to pig farmers and abattoir workers. Similar scenarios were seen in Australia where Pteropus bats transmitted Hendra virus to horses as intermediate hosts for human infections. Pteropus bats contaminated palm sap collected in Bangladesh with Nipah virus where fatal human-to-human transmissions occurred annually. Less direct evidence links coronaviruses carried by Rhinolophus bats with SARS and COVID-19 pandemics and a piglet epidemic in China. Rousettus bats living in caves transmitted the Marburg virus (family: filovirus) to miners in Africa. Most cases of human rabies in North America were caused by bat lyssaviruses (family: Rhabdoviruses). Bats are viral reservoir species for various virus families (reovirus, Hepacivirus of Flavivirus family, influenza A viruses). Bats are the only flying mammals which opened enormous evolutionary possibilities resulting in a worldwide radiation with 1400 species. Some bat species are represented by huge populations that come together in extremely crowded resting places that are conducive to viral transmission. Bats have evolved mechanisms that tolerate virus replication but suppress the associated pathology, making them healthy carriers for many viruses. It is speculated that with that strategy bats avoid an arms race with viruses for resistance and anti-resistance mechanisms. The excretion of viruses that are highly pathogenic for other mammalian orders could be used as biological weapons to defend their habitat against intrusion by mammalian competitors, including humans. This hypothesis might explain the increasing involvement of bat viruses in emerging infectious diseases observed in recent decades and expected in the future.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to A Single-Plasmid Inducible-Replication System for High-Yield Production of Short Ff (f1, M13 or fd)-Phage-Derived Nanorods","authors":"","doi":"10.1111/1751-7915.70192","DOIUrl":"10.1111/1751-7915.70192","url":null,"abstract":"<p>León-Quezada, R. I., M. G. Miró, S. Khanum, A. J. Sutherland-Smith, V. A. M. Gold, and J. Rakonjac. 2025. Microbial Biotechnology, 18: e70113. https://doi.org/10.1111/1751-7915.70113</p><p>In Supplementary Information file <i>mbt270113-sup-0001-supinfo.docx</i>, the image (graphics) of Fig. S6 on P7 is incorrect.</p><p>We apologise for this error.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Characterisation of the upp Gene in Bifidobacterium bifidum PRL2010","authors":"Giulia Longhi,&nbsp;Silvia Petraro,&nbsp;Christian Milani,&nbsp;Chiara Tarracchini,&nbsp;Chiara Argentini,&nbsp;Laura Maria Vergna,&nbsp;Gabriele Andrea Lugli,&nbsp;Leonardo Mancabelli,&nbsp;Ciaran Lee,&nbsp;Francesca Turroni,&nbsp;Douwe van Sinderen,&nbsp;Marco Ventura","doi":"10.1111/1751-7915.70189","DOIUrl":"10.1111/1751-7915.70189","url":null,"abstract":"<p>Bifidobacteria are key members of the human gut, especially during infancy. The ability of bifidobacteria to outcompete other members of the microbial communities encountered in this highly competitive human gut environment represents a key example of their evolutionary and ecological success. In the current report, we investigated the highly conserved bifidobacterial <i>upp</i> gene, which encodes the uracil phosphoribosyltransferase and which is involved in the pyrimidine salvage pathway. Phylogenetic analysis incorporating 107 bifidobacterial <i>upp</i> sequences, representing all currently known <i>Bifidobacterium</i> taxa, indicates that this gene followed an evolutionary route that apparently deviates from that of the 16S rRNA gene. In addition, the <i>upp</i> gene may support bifidobacterial survival in environments with limited uracil availability, potentially providing a competitive advantage under nutrient-restricted conditions.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Urea Production in the Diazotroph Azotobacter vinelandii as a Means of Stable Nitrogen Biofertiliser Production","authors":"Brett M. Barney,&nbsp;Benjamin R. Dietz","doi":"10.1111/1751-7915.70187","DOIUrl":"10.1111/1751-7915.70187","url":null,"abstract":"<p>Diazotrophic microbes capture atmospheric nitrogen and convert it into ammonia using the enzyme nitrogenase in a process that provides much of the fixed nitrogen that is required to sustain life in the biosphere. The advent of the Haber Bosch industrial process in the 20th century ushered in an age when agricultural productivity could circumvent the constraints of biological nitrogen fixation, leading to higher productivity based on chemical fertilisers. This industrial process now provides a substantial amount of the nitrogen that we apply to crops, but comes with a large environmental and economic cost. In contrast, biological nitrogen fixation still contributes nitrogen to crops and has the potential to displace some of the industrial nitrogen if we can engineer methods to increase nitrogen levels that are provided to the plant or develop stronger associations between diazotrophs and nonlegume plants. Many of the processes scientists have employed to enhance the nitrogen production by diazotrophs to develop improved biofertilisers have focused on delivering nitrogen in the form of ammonium. In this report, we describe an alternative approach that provides the nitrogen as urea in the form of a terminal product. Using the model diazotroph <i>Azotobacter vinelandii</i> and a three-step approach that deletes the native urease, incorporates a functional arginase and overcomes the feedback inhibition of the arginine biosynthesis pathway, we have increased levels of urea that could be obtained from previous approaches by approximately 43-fold. Our results demonstrate the ability to support the growth of a green alga with these engineered strains and yield total extracellular nitrogen that is comparable to what has been achieved with ammonium.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70187","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Different Transposon Families on Genomic Stability of Shewanella oneidensis MR1","authors":"Benjamin Fritz,&nbsp;Christian Jonas Lapp,&nbsp;Johannes Gescher","doi":"10.1111/1751-7915.70188","DOIUrl":"10.1111/1751-7915.70188","url":null,"abstract":"<p><i>Shewanella oneidensis</i>, recognised as an important model organism for exoelectrogenic electron transport, has been extensively studied for its potential applications in bioelectrochemical systems. To date, the activity of transposable elements in this organism has not been conclusively investigated. This study focused on transposases, specifically insertion sequences (IS), which make up approximately 4.7% of the organism's genome, and evaluated their impact on genome stability under stress conditions. Using whole genome sequencing, two IS families, ISSOD1 and ISSOD2, were identified as the most active, both showing similar transposition patterns across all tested stressors. A CRISPR/dCas9 cytosine deaminase system was used to introduce stop codons in the ISSOD2 transposase genes, resulting in a significant reduction of transposition events under stress conditions. Analysis of transposition patterns revealed a high frequency of insertions occurring on the megaplasmid, which predominantly carries non-essential genes. Experiments performed here to delete the megaplasmid resulted in the elimination of approximately 35% of its sequence, including an unexpected complete loss of the ori/repA region. Therefore, it was hypothesised that the megaplasmid either exists in a metastable state, possibly representing a cointegrated intermediate within the ISSOD9 (Tn3 member) transposition mechanism, or consists of two replicons that have been combined in previous assemblies due to long overlapping homologies resulting from the presence of ISSOD9. These findings highlight the dynamics of transposable elements in <i>S. oneidensis</i> and suggest strategies to improve strain stability by inactivating these elements and at least reducing megaplasmid sequences. Such approaches could improve the suitability of the organism for industrial applications.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Dual ‘RT-LAMP-LFA’ Rapid Detection Technology With Gold Magnetic Nanoparticles for Influenza Virus","authors":"Haiyang Fan,&nbsp;Yonglong Gong,&nbsp;Mengying Chang,&nbsp;Juan Gao,&nbsp;Mengjia Li,&nbsp;Siyu Chen,&nbsp;Ruoyi Yang,&nbsp;Muxue Zhao,&nbsp;Yali Cui,&nbsp;Wenli Hui","doi":"10.1111/1751-7915.70169","DOIUrl":"10.1111/1751-7915.70169","url":null,"abstract":"<p>Seasonal and persistent outbreaks of influenza viruses represent a significant challenge to global public health. Rapid, convenient and accurate diagnosis methods of influenza viruses are crucial for timely treatment to mitigate morbidity and mortality during both seasonal epidemics and pandemics. However, current diagnostic tools often face limitations in speed, accuracy or complexity of result interpretation; there is a great need for more efficient detection technology for influenza virus, especially for use in resource-limited settings or during large-scale outbreaks. This study developed a dual ‘RT-LAMP-LFA’ detection technology with gold magnetic nanoparticles for influenza virus. This method can simultaneously detect influenza A and B genes as well as internal reference genes within 35 min, with a detection limit of 80 copies/mL. This is the first time the RNase P gene has been introduced into a gold magnetic nanoparticle lateral flow assay system as a quality control measure to monitor the entire sampling and amplification process in virus detection and reveals the effects of loop primer deficiencies on the stability of the dual ‘RT-LAMP-LFA’ detection technology. Using fluorescent PCR detection technology as a benchmark, the analysis of a total of 70 clinical samples demonstrated a 100% agreement rate, confirming the applicability and accuracy of the dual ‘RT-LAMP-LFA’ detection system. This dual ‘RT-LAMP-LFA’ detection technology offers a novel option for diagnostic technology in hierarchical medical testing, presenting significant social importance and broad application prospects.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigations of the Flavin-Dependent Monooxygenase PhzO Involved in Phenazine Biosynthesis","authors":"Yan-Fang Nie,&nbsp;Sheng-Jie Yue,&nbsp;Peng Huang,&nbsp;Xue-Hong Zhang,&nbsp;Xiang-Rui Hao,&nbsp;Lian Jiang,&nbsp;Hong-Bo Hu","doi":"10.1111/1751-7915.70186","DOIUrl":"10.1111/1751-7915.70186","url":null,"abstract":"<p>Phenazines are bioactive secondary metabolites with antifungal, anticancer, and insecticidal properties, while hydroxylated derivatives often exhibit enhanced bioactivity. 2-hydroxyphenazine (2-OH-PHZ), which is synthesised by the flavin-dependent monooxygenase PhzO from phenazine-1-carboxylic acid (PCA), shows better bioactivity against the pathogenic fungus <i>Gaeumannomyces graminis</i> vars. <i>tritici</i>. However, the low catalytic efficiency (10%–20% conversion) of PhzO limited 2-OH-PHZ production. To boost PhzO activity, engineering flavin reductase (Fre)-mediated FADH₂ regeneration was applied to <i>Pseudomonas chlororaphis</i> LX24AE. Remarkably, this approach improved catalytic efficiency from 25% to 40% and increased the production of a novel dihydroxylated derivative. Then, it was first characterised by UPLC-MS and NMR, and identified as 3,4-dihydroxyphenazine-1-carboxylic acid (3,4-OH-PCA). Next, the Fre-PhzO module through heterologous co-expression in <i>P. putida</i> KT2440 demonstrated a 4.5-fold enhancement in hydroxylation efficiency relative to the PhzO mono-component system, which also confirmed that PhzO and flavin reductase are essential for 3,4-OH-PCA biosynthesis. Moreover, in vitro assays further verified that PhzO exhibits FAD-dependent catalytic promiscuity, simultaneously generating 2-OH-PCA and 3,4-OH-PCA. Furthermore, in vitro and in vivo assay<i>s</i> demonstrated that substrate concentration affected the distribution of products. Finally, cytotoxicity evaluation of the isolated 3,4-OH-PCA was performed, and it showed substantial inhibition against oesophageal cancer TE-1 cells and human cervical cancer HeLa cells with an IC₅₀ value of 8.55 μM and 17.69 μM, respectively. This work redefines PhzO as a promiscuous biocatalyst capable of dual hydroxylation, offering a modular platform for engineering bioactive phenazine derivatives.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70186","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}