Microbial Biotechnology最新文献

{"title":"Gut Microbiota-Derived Acetate Ameliorates Endometriosis via JAK1/STAT3-Mediated M1 Macrophage Polarisation","authors":"Yunyun Xu,&nbsp;Yichen Zhu,&nbsp;Xiaoyun Wu,&nbsp;Wan Peng,&nbsp;Yanying Zhong,&nbsp;Yujie Cai,&nbsp;Wenjing Chen,&nbsp;Lu Liu,&nbsp;BuZhen Tan,&nbsp;Tingtao Chen","doi":"10.1111/1751-7915.70202","DOIUrl":"https://doi.org/10.1111/1751-7915.70202","url":null,"abstract":"<p>Endometriosis (EMs) is a common inflammatory disorder in women of reproductive age, severely impacting patients' quality of life and fertility. Current hormonal therapies offer limited efficacy, and surgical interventions often fail to prevent recurrence. Recent studies suggest a close association between gut microbiota and the pathophysiology of EMs, though the precise mechanisms remain unclear. To investigate the influence of gut microbiota on EMs, this study established an EMs mouse model and performed faecal microbiota transplantation (FMT) using samples from healthy donors (AH group) and EMs patients (AE group) into the model mice. Results demonstrated that compared to the model group (M group), FMT from healthy donors (AH group) significantly reduced ectopic lesion volume (658.3 ± 116.1 vs. 167.2 ± 112.8 mm³, <i>p</i> &lt; 0.01) and weight (0.7420 ± 0.1233 vs. 0.1885 ± 0.1239 mg, <i>p</i> &lt; 0.01). Conversely, FMT from EMs patients exacerbated disease progression. Mechanistic studies revealed that healthy donor FMT attenuated EMs by remodelling the gut microbial composition (enhancing α-diversity and <i>Lactobacillus</i> abundance while suppressing Bacteroidetes), significantly elevating acetate levels in faeces and ectopic lesions, activating the JAK1/STAT3 signalling pathway within lesions, and thereby driving macrophage polarisation toward the M1 phenotype (by increased iNOS/CD86 expression and decreased Arg1/CD206 expression). Simultaneously, healthy donor FMT enhanced intestinal barrier integrity by upregulating tight junction proteins (ZO-1, Occludin, Claudin-1/5) and reducing levels of intestinal permeability markers (DAO, IFABP). In contrast, AE group FMT disrupted gut microbial ecology, reduced acetate production, failed to activate the JAK1/STAT3 pathway, promoted M2 macrophage polarisation and impaired intestinal barrier function. Collectively, this study elucidates for the first time that acetate, as a key gut microbiota metabolite, exerts anti-EMs effects by activating the JAK1/STAT3 signalling pathway to drive macrophage reprogramming toward the M1 phenotype, thereby positioning gut microbiota reconstruction as a novel therapeutic strategy for endometriosis.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70202","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Role of MtHAC-1 in Regulating Cellulase and Xylanase Production in Myceliophthora thermophila","authors":"Yapeng Lai,&nbsp;Juan Wang,&nbsp;Ning Xie,&nbsp;Gang Liu,&nbsp;Donnabella C. Lacap-Bugler","doi":"10.1111/1751-7915.70203","DOIUrl":"https://doi.org/10.1111/1751-7915.70203","url":null,"abstract":"<p>Filamentous fungi produce large quantities of cellulase and xylanase as extracellular enzymes to degrade plant-derived polysaccharides. This process is controlled by a complex network of transcription factors (TFs). Here, we present the bZIP TF Mthac-1 exhibiting dual regulatory effects on the production of cellulase and xylanase in <i>Myceliophthora thermophila</i>. The deletion of <i>Mthac-1</i> reduced the cellulase and xylanase activities and protein secretion during the early phase of cultivation but enhanced in the middle and late stages of cultivation, compared with the wild-type (WT) strain. It also led to fungal growth defects, characterised by few hyphal branching and reduced conidiation. Real-time quantitative reverse transcription PCR (RT-qPCR) analysis showed that Mthac-1 dynamically regulates the expression of major cellulase genes. Furthermore, electrophoretic mobility shift assays (EMSAs) demonstrated that Mthac-1 directly binds to the promoter regions of the β-glucosidase gene <i>bgl1</i> (<i>MYCTH_66804</i>), cellobiohydrolase gene <i>cbh1</i> (<i>MYCTH_109566</i>), endoglucanase gene <i>egl2</i> (<i>MYCTH_86753</i>), xylanase gene <i>xyn1</i> (<i>MYCTH_112050</i>) and the regulatory gene <i>xyr1</i> (<i>MYCTH_2310145</i>), exhibiting higher binding affinity for <i>xyn1</i> and <i>xyr1</i>. The comparative transcriptomic analysis indicated that Mthac-1 also plays an important role in the expression of 26S proteasome-encoding genes under cellulolytic conditions. This work provides new insights into the regulatory mechanisms underlying cellulase and xylanase gene expression with potential applications in fungal strain engineering in biorefinery industries.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiota-Derived Acetate Ameliorates Endometriosis via JAK1/STAT3-Mediated M1 Macrophage Polarisation","authors":"Yunyun Xu,&nbsp;Yichen Zhu,&nbsp;Xiaoyun Wu,&nbsp;Wan Peng,&nbsp;Yanying Zhong,&nbsp;Yujie Cai,&nbsp;Wenjing Chen,&nbsp;Lu Liu,&nbsp;BuZhen Tan,&nbsp;Tingtao Chen","doi":"10.1111/1751-7915.70202","DOIUrl":"10.1111/1751-7915.70202","url":null,"abstract":"<p>Endometriosis (EMs) is a common inflammatory disorder in women of reproductive age, severely impacting patients' quality of life and fertility. Current hormonal therapies offer limited efficacy, and surgical interventions often fail to prevent recurrence. Recent studies suggest a close association between gut microbiota and the pathophysiology of EMs, though the precise mechanisms remain unclear. To investigate the influence of gut microbiota on EMs, this study established an EMs mouse model and performed faecal microbiota transplantation (FMT) using samples from healthy donors (AH group) and EMs patients (AE group) into the model mice. Results demonstrated that compared to the model group (M group), FMT from healthy donors (AH group) significantly reduced ectopic lesion volume (658.3 ± 116.1 vs. 167.2 ± 112.8 mm³, <i>p</i> &lt; 0.01) and weight (0.7420 ± 0.1233 vs. 0.1885 ± 0.1239 mg, <i>p</i> &lt; 0.01). Conversely, FMT from EMs patients exacerbated disease progression. Mechanistic studies revealed that healthy donor FMT attenuated EMs by remodelling the gut microbial composition (enhancing α-diversity and <i>Lactobacillus</i> abundance while suppressing Bacteroidetes), significantly elevating acetate levels in faeces and ectopic lesions, activating the JAK1/STAT3 signalling pathway within lesions, and thereby driving macrophage polarisation toward the M1 phenotype (by increased iNOS/CD86 expression and decreased Arg1/CD206 expression). Simultaneously, healthy donor FMT enhanced intestinal barrier integrity by upregulating tight junction proteins (ZO-1, Occludin, Claudin-1/5) and reducing levels of intestinal permeability markers (DAO, IFABP). In contrast, AE group FMT disrupted gut microbial ecology, reduced acetate production, failed to activate the JAK1/STAT3 pathway, promoted M2 macrophage polarisation and impaired intestinal barrier function. Collectively, this study elucidates for the first time that acetate, as a key gut microbiota metabolite, exerts anti-EMs effects by activating the JAK1/STAT3 signalling pathway to drive macrophage reprogramming toward the M1 phenotype, thereby positioning gut microbiota reconstruction as a novel therapeutic strategy for endometriosis.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70202","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Role of MtHAC-1 in Regulating Cellulase and Xylanase Production in Myceliophthora thermophila","authors":"Yapeng Lai,&nbsp;Juan Wang,&nbsp;Ning Xie,&nbsp;Gang Liu,&nbsp;Donnabella C. Lacap-Bugler","doi":"10.1111/1751-7915.70203","DOIUrl":"10.1111/1751-7915.70203","url":null,"abstract":"<p>Filamentous fungi produce large quantities of cellulase and xylanase as extracellular enzymes to degrade plant-derived polysaccharides. This process is controlled by a complex network of transcription factors (TFs). Here, we present the bZIP TF Mthac-1 exhibiting dual regulatory effects on the production of cellulase and xylanase in <i>Myceliophthora thermophila</i>. The deletion of <i>Mthac-1</i> reduced the cellulase and xylanase activities and protein secretion during the early phase of cultivation but enhanced in the middle and late stages of cultivation, compared with the wild-type (WT) strain. It also led to fungal growth defects, characterised by few hyphal branching and reduced conidiation. Real-time quantitative reverse transcription PCR (RT-qPCR) analysis showed that Mthac-1 dynamically regulates the expression of major cellulase genes. Furthermore, electrophoretic mobility shift assays (EMSAs) demonstrated that Mthac-1 directly binds to the promoter regions of the β-glucosidase gene <i>bgl1</i> (<i>MYCTH_66804</i>), cellobiohydrolase gene <i>cbh1</i> (<i>MYCTH_109566</i>), endoglucanase gene <i>egl2</i> (<i>MYCTH_86753</i>), xylanase gene <i>xyn1</i> (<i>MYCTH_112050</i>) and the regulatory gene <i>xyr1</i> (<i>MYCTH_2310145</i>), exhibiting higher binding affinity for <i>xyn1</i> and <i>xyr1</i>. The comparative transcriptomic analysis indicated that Mthac-1 also plays an important role in the expression of 26S proteasome-encoding genes under cellulolytic conditions. This work provides new insights into the regulatory mechanisms underlying cellulase and xylanase gene expression with potential applications in fungal strain engineering in biorefinery industries.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Genomic Assessment of the Cupriavidus necator Species for One-Carbon Based Biomanufacturing","authors":"Magnus G. Jespersen,&nbsp;Emil Funk Vangsgaard,&nbsp;Mariana Arango Saavedra,&nbsp;Stefano Donati,&nbsp;Lars K. Nielsen","doi":"10.1111/1751-7915.70201","DOIUrl":"10.1111/1751-7915.70201","url":null,"abstract":"<p>The transition from a petroleum-based manufacturing to biomanufacturing is an important step towards a sustainable bio-economy. In particular, biotechnological processes which use one carbon (C1) compounds as feedstock represent an interesting avenue. Many bacterial species evolved naturally to thrive on such compounds, among them <i>Cupriavidus necator,</i> which has been studied in the past due to its range of metabolic capabilities in utilisation and production of compounds of interest. <i>Cupriavidus necator</i> strain H16 is the reference laboratory strain for this species and by far the most extensively studied. In contrast, research efforts and genomic characterisation of other strains within this species have been limited and sporadic. Therefore, the genomic diversity and full metabolic potential across the broader species remain poorly understood. In this work, we collected publicly available genomes along with newly sequenced ones. From a collection of 44 genomes, we curated a final collection of 22 genomes deemed to be <i>C. necator</i>. We examined hallmark metabolic functions, including carbon dioxide fixation, formate assimilation and hydrogen utilisation. We identified methylation motifs and restriction modification systems. Finally, strains ATCC 25207, TA06, and 1978 are proposed as candidate strains of interest based on their genomic make-up and observations from literature. This work provides a comprehensive genomic resource for the <i>C. necator</i> species, facilitating its development as a biomanufacturing platform and advancing our understanding of its metabolic diversity and potential applications.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lyophilised Reverse Transcriptase and Polymerase for Point-of-Care Diagnostics of SARS-CoV-2","authors":"Natalie Mutter,&nbsp;Barbara Posch,&nbsp;Yasmin Gillitschka,&nbsp;Nadezhda Kataeva,&nbsp;Birgit Michalek,&nbsp;Daniel Lager,&nbsp;Filip Staniszewski,&nbsp;Alexandra Schilder,&nbsp;Lidiya Osinkina,&nbsp;Maximilian Westenthanner,&nbsp;Joachim Stehr,&nbsp;Federico Buersgens,&nbsp;Johannes R. Peham","doi":"10.1111/1751-7915.70200","DOIUrl":"10.1111/1751-7915.70200","url":null,"abstract":"<p>Early diagnosis of pathogens is key to reducing their spreading and to preventing severe health risks. The COVID-19 pandemic showed us the need for rapid point-of-care tests. Here, we describe the preparation of an amplification master mix for point-of-care diagnostics. Therefore, two off-patent amplification enzymes were designed, expressed and purified. The preparation of the key components enables independence from delivery issues, manufacturer portfolio and product information's. For long-term storage and cold-free transport, our fabricated amplification mix was lyophilised. Finally, we applied our lyophilised master mix on an integrated point-of-care diagnostic system and could detect 10 copies/μL COVID-19 RNA. The combination of stable, cold-free reagents with the mobile and low-cost device will allow molecular diagnostics of pathogens in a field or home setting.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70200","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergy of ATP and Meropenem in Stimulating the TCA Cycle to Enhance Killing of Carbapenem-Resistant Acinetobacter baumannii","authors":"Xia Li,&nbsp;Dingyun Feng,&nbsp;Jianxia Zhou,&nbsp;Wenbin Wu,&nbsp;Chunyan He,&nbsp;Wenlei Gan,&nbsp;Wenzheng Zheng,&nbsp;Bo Peng,&nbsp;Tiantuo Zhang","doi":"10.1111/1751-7915.70199","DOIUrl":"10.1111/1751-7915.70199","url":null,"abstract":"<p>The global prevalence of carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) represents a substantial concern for public health. Exogenous adenosine triphosphate (ATP) affects the bactericidal efficacy of meropenem against CRAB; however, the precise mechanism remains elusive. Here, reprogramming metabolomics was employed to delve into the mechanism underlying ATP-potentiated meropenem lethality against CRAB. Our findings reveal that ATP reprogramming activates the tricarboxylic acid (TCA) cycle in <i>A. baumannii</i>. Notably, the TCA cycle inhibitor malonate antagonised the synergistic bactericidal effect of ATP and meropenem. Activation of the TCA cycle stimulated riboflavin metabolism pathway and the electron transport chain, leading to increased reactive oxygen species (ROS) production. Hydrogen peroxide (H₂O₂) enhanced meropenem-mediated killing of CRAB, while the ROS scavenger α-tocopherol diminished the ATP-potentiated bactericidal effect. Additionally, ATP upregulated the gene expression of outer membrane porins, including <i>omp33-36</i>, <i>oprD</i>, <i>ompW</i>, and <i>ompA</i>, thereby improving membrane permeability and elevating intracellular levels of meropenem. The therapeutic synergy of ATP with meropenem was validated in a mouse model of acute pneumonia. This study not only highlights the potential of ATP as a co-treatment with meropenem but also elucidates the mechanisms by which ATP reverses CRAB resistance, specifically through the promotion of ROS production and enhanced membrane permeability.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70199","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbes as Teachers: Rethinking Knowledge in the Anthropocene","authors":"Rachel Armstrong","doi":"10.1111/1751-7915.70195","DOIUrl":"10.1111/1751-7915.70195","url":null,"abstract":"<p>This opinion piece proposes that the environmental crises of our time arise from a failure to recognise the vital role of microbes in sustaining life on Earth, where ecosystems have been shaped for billions of years by microbial processes, including oxygen production, nutrient cycling and climate regulation. Yet the idea that microbes can ‘teach’ us how to navigate complexity, adapt across scales, and sustain planetary systems is still marginalised in science, policy, and education. A paradigm shift is proposed: microbes must be reframed as active collaborators in solving global challenges. This perspective is grounded in microbial ecology, Indigenous knowledge, and ethical philosophy, advocating for ‘learning’ through and with microbial life. To institutionalise this transition, policy and educational reforms are urged, centring microbial literacy as a foundation for ecological understanding. By integrating microbial agency into human knowledge systems, societal actions could be realigned with the biochemical and evolutionary logics that have sustained life for millennia. Ultimately, a deeper engagement with microbial knowledge is called for—one that informs a more sustainable future.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Biotechnology as a Catalyst for a Better and More Sustainable World","authors":"Carmen Michán,&nbsp;Juan L. Ramos","doi":"10.1111/1751-7915.70196","DOIUrl":"10.1111/1751-7915.70196","url":null,"abstract":"<p>Education is our most powerful tool for transforming society and improving human well-being. <i>Microbial Biotechnology</i> has previously addressed how we can contribute to enhance public understanding of microbiology in general, and microbial biotechnology in particular, along with their potential benefits. Here, we summarise several contributions in the Special issue ‘Societally-relevant microbiology and outreach’.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Endosymbiosis of Pedobacter sp. DDGJ Boosts the Growth Potential, Stress Resistance and Productivity of Morchella Mushrooms","authors":"Wenchang Zhang,&nbsp;Mantang Cao,&nbsp;Qi Yin,&nbsp;Jin Zhang,&nbsp;Chenhui Wang,&nbsp;Li Han,&nbsp;Yingli Cai,&nbsp;Xiaofei Shi,&nbsp;Linjing Ma,&nbsp;Yousif Abdelrahman Yousif Abdellah,&nbsp;Fuqiang Yu,&nbsp;Peixin He,&nbsp;Wei Liu","doi":"10.1111/1751-7915.70197","DOIUrl":"10.1111/1751-7915.70197","url":null,"abstract":"<p>True morels are precious edible and medicinal mushrooms. The sustainable development of morel cultivation necessitates urgent breeding programmes to develop improved varieties or strains. Some soil bacteria, including <i>Pedobacter</i> spp., are recognised as morel's beneficial microbes. In this paper, the potential beneficial bacterium <i>Pedobacter</i> sp. DDGJ with minor chitinolytic activity was isolated and confirmed for improving morel's mycelial growth and identified using a combination of morphological characteristics, 16 S rRNA gene sequencing, and comparative genomics. Then, it was introduced into the hyphal cells of three cultivated <i>Morchella</i> strains (<i>M. sextelata</i> 13, <i>M. eximia</i> SM, and <i>M. importuna</i> Y2) through a confrontation culture method. The successful establishment of artificial endosymbiosis was confirmed by PCR assay, green fluorescent protein gene (<i>gfp</i>) localisation, and the cryo-preparation system conjunction with high-resolution field emission scanning electron microscopy. Laboratory assay indicated that artificial endosymbiosis of the bacterium significantly enhanced morel's growth potential, resistance to allelochemicals of 4-coumaric acid and antagonistic capability against the pathogenic fungus <i>Fusarium oxysporum</i> and soil-dominant fungus <i>Chaetomium globosum</i>. Moreover, the outdoor planting experiment showed significant increase in productivity among the endosymbiotic morel strains. The study provides an additional tool to improve <i>Morchella</i> strains with high yield and strong stress resistance.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 7","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}