Microbial Biotechnology最新文献_第7页

Correction to ‘Multiple Chaperone DnaK–FliC Flagellin Interactions Are Required for Pseudomonas aeruginosa Flagellum Assembly and Indicate a New Function for DnaK’ 更正“铜绿假单胞菌鞭毛组装需要多个伴侣dna - flc鞭毛蛋白相互作用，并表明DnaK的新功能”

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-26 DOI: 10.1111/1751-7915.70138

{"title":"Correction to ‘Multiple Chaperone DnaK–FliC Flagellin Interactions Are Required for Pseudomonas aeruginosa Flagellum Assembly and Indicate a New Function for DnaK’","authors":"","doi":"10.1111/1751-7915.70138","DOIUrl":"https://doi.org/10.1111/1751-7915.70138","url":null,"abstract":"Molinari, G., S. S. Ribeiro, K. Müller, et al. 2025. “Multiple Chaperone DnaK–FliC Flagellin Interactions Are Required for Pseudomonas aeruginosa Flagellum Assembly and Indicate a New Function for DnaK.” Microbial Biotechnology 18, no. 2: e70096. https://doi.org/10.1111/1751-7915.70096.In paragraph 4 of the ‘3.8 | DnaK Functions in an ATP-Independent Manner at Both Physiological and Mild Acidic pHs’ subsection in the ‘Results’ section, the equation ‘ΔD/AInitial = D/AiDnaKD − AiSOD1’ was incorrect. This should have read: ‘ΔD/AInitial = D/AiDnaK − D/AiSOD1’.In caption of Figure 6 (C) of the ‘Results’ section, the equation ‘ΔD/AInitial = D/AiDnaK/NucleotidesD/AiSOD1’ was incorrect. This should have read: ‘ΔD/AInitial = D/AiDnaK/Nucleotides − D/AiSOD1’.We apologise for this error.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecology and Evolutionary Biology as Frameworks to Study Wine Fermentations 生态学和进化生物学作为研究葡萄酒发酵的框架

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-26 DOI: 10.1111/1751-7915.70078

Ignacio Belda, Belen Benitez-Dominguez, Sergio Izquierdo-Gea, Jean C. C. Vila, Javier Ruiz

引用次数: 0

Understanding Efflux-Mediated Multidrug Resistance in Botrytis cinerea for Improved Management of Fungicide Resistance 了解葡萄孢外排介导的多药耐药，以改善对杀菌剂耐药性的管理

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-25 DOI: 10.1111/1751-7915.70074

Zhaochen Wu, Junting Zhang, Jianjun Hao, Pengfei Liu, Xili Liu

引用次数: 0

Understanding Efflux-Mediated Multidrug Resistance in Botrytis cinerea for Improved Management of Fungicide Resistance 了解葡萄孢外排介导的多药耐药，以改善对杀菌剂耐药性的管理

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-25 DOI: 10.1111/1751-7915.70074

Zhaochen Wu, Junting Zhang, Jianjun Hao, Pengfei Liu, Xili Liu

引用次数: 0

Metagenomic Exploration Uncovers Several Novel ‘Candidatus’ Species Involved in Acetate Metabolism in High-Ammonia Thermophilic Biogas Processes 宏基因组探索揭示了在高氨嗜热沼气过程中参与醋酸代谢的几种新的“候选菌”物种

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-24 DOI: 10.1111/1751-7915.70133

George B. Cheng, Erik Bongcam-Rudloff, Anna Schnürer

{"title":"Metagenomic Exploration Uncovers Several Novel ‘Candidatus’ Species Involved in Acetate Metabolism in High-Ammonia Thermophilic Biogas Processes","authors":"George B. Cheng, Erik Bongcam-Rudloff, Anna Schnürer","doi":"10.1111/1751-7915.70133","DOIUrl":"https://doi.org/10.1111/1751-7915.70133","url":null,"abstract":"Biogas reactors operating at elevated ammonia levels are commonly susceptible to process disturbances, further augmented at thermophilic temperatures. The major cause is assumed to be linked to inhibition followed by an imbalance between different functional microbial groups, centred around the last two steps of the anaerobic digestion, involving acetogens, syntrophic acetate oxidisers (SAOB) and methanogens. Acetogens are key contributors to reactor efficiency, acting as the crucial link between the hydrolysis and fermentation steps and the final methanogenesis step. Their major product is acetate, at high ammonia levels further converted by SAOB and hydrogenotrophic methanogens to biogas. Even though these functionally different processes are well recognised, less is known about the responsible organism at elevated temperature and ammonia conditions. The main aim of this study was to garner insights into the penultimate stages in three thermophilic reactors (52°C) operated under high ammonia levels (FAN 0.7–1.0 g/L; TAN 3.6–4.4 g/L). The primary objective was to identify potential acetogens and SAOBs. Metagenomic data from the three reactors were analysed for the reductive acetyl-CoA pathway (Wood–Ljungdahl Pathway) and glycine synthase reductase pathway. The results revealed a lack of true acetogens but uncovered three potential SAOB candidates that harbour the WLP, ‘Candidatus Thermodarwinisyntropha acetovorans’, ‘Candidatus Thermosyntrophaceticus schinkii’, ‘Candidatus Thermotepidanaerobacter aceticum’, and a potential lipid-degrader ‘Candidatus Thermosyntrophomonas ammoiaca’.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic Exploration Uncovers Several Novel ‘Candidatus’ Species Involved in Acetate Metabolism in High-Ammonia Thermophilic Biogas Processes 元基因组探索发现了几种参与高氨嗜热沼气工艺中乙酸代谢的新型 "念珠菌 "物种

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-24 DOI: 10.1111/1751-7915.70133

George B. Cheng, Erik Bongcam-Rudloff, Anna Schnürer

{"title":"Metagenomic Exploration Uncovers Several Novel ‘Candidatus’ Species Involved in Acetate Metabolism in High-Ammonia Thermophilic Biogas Processes","authors":"George B. Cheng, Erik Bongcam-Rudloff, Anna Schnürer","doi":"10.1111/1751-7915.70133","DOIUrl":"https://doi.org/10.1111/1751-7915.70133","url":null,"abstract":"Biogas reactors operating at elevated ammonia levels are commonly susceptible to process disturbances, further augmented at thermophilic temperatures. The major cause is assumed to be linked to inhibition followed by an imbalance between different functional microbial groups, centred around the last two steps of the anaerobic digestion, involving acetogens, syntrophic acetate oxidisers (SAOB) and methanogens. Acetogens are key contributors to reactor efficiency, acting as the crucial link between the hydrolysis and fermentation steps and the final methanogenesis step. Their major product is acetate, at high ammonia levels further converted by SAOB and hydrogenotrophic methanogens to biogas. Even though these functionally different processes are well recognised, less is known about the responsible organism at elevated temperature and ammonia conditions. The main aim of this study was to garner insights into the penultimate stages in three thermophilic reactors (52°C) operated under high ammonia levels (FAN 0.7–1.0 g/L; TAN 3.6–4.4 g/L). The primary objective was to identify potential acetogens and SAOBs. Metagenomic data from the three reactors were analysed for the reductive acetyl-CoA pathway (Wood–Ljungdahl Pathway) and glycine synthase reductase pathway. The results revealed a lack of true acetogens but uncovered three potential SAOB candidates that harbour the WLP, ‘Candidatus Thermodarwinisyntropha acetovorans’, ‘Candidatus Thermosyntrophaceticus schinkii’, ‘Candidatus Thermotepidanaerobacter aceticum’, and a potential lipid-degrader ‘Candidatus Thermosyntrophomonas ammoiaca’.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design Assisted by Evolutionary Engineering Allows (De)Construction and Optimization of Complex Phenotypes in Pseudomonas putida KT2440 进化工程辅助下的理性设计允许恶臭假单胞菌KT2440复杂表型的构建和优化

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-24 DOI: 10.1111/1751-7915.70132

Blas Blázquez, Juan Nogales

{"title":"Rational Design Assisted by Evolutionary Engineering Allows (De)Construction and Optimization of Complex Phenotypes in Pseudomonas putida KT2440","authors":"Blas Blázquez, Juan Nogales","doi":"10.1111/1751-7915.70132","DOIUrl":"https://doi.org/10.1111/1751-7915.70132","url":null,"abstract":"Beyond the rational construction of genetic determinants to encode target functions, complex phenotype engineering requires the contextualisation of their expression within the metabolic and genetic background of the host strain. Furthermore, wherever metabolic complexity is involved, phenotype engineering demands standard, reliable, plug-and-play tools. We introduce GENIO (GENome Integration and fitness Optimization platform for Pseudomonas putida), a framework to optimise genetic circuit performance by means of (i) chromosome-location-based differential gene expression and (ii) subsequent fitness improvement through evolutionary engineering if needed. Using gene expression strength and cell-to-cell variation, we characterised 10 P. putida chromosomal loci (ppLPS) to show that genome context rather than distance to ORI is the main factor driving differential expression performance. We further contextualised ppLPS gene expression against well-known chromosomal integration sites and plasmids displaying different copy numbers. GENIO supports comprehensive exploration of the gene expression space across P. putida's genome while unlocking performance optimization of complex heterologous metabolic pathways through evolutionary engineering. To demonstrate the usability of GENIO, we restored P. putida's aromatic hydrocarbon metabolism by (de)constructing the toluene/m-xylene catabolic pathway coded in the pWW0 plasmid. We also showed that engineering complex phenotypes requires accurate contextualisation of the synthetic pathways involved, a process that benefits from biological robustness.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design Assisted by Evolutionary Engineering Allows (De)Construction and Optimization of Complex Phenotypes in Pseudomonas putida KT2440 进化工程辅助下的理性设计允许恶臭假单胞菌KT2440复杂表型的构建和优化

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-24 DOI: 10.1111/1751-7915.70132

Blas Blázquez, Juan Nogales

{"title":"Rational Design Assisted by Evolutionary Engineering Allows (De)Construction and Optimization of Complex Phenotypes in Pseudomonas putida KT2440","authors":"Blas Blázquez, Juan Nogales","doi":"10.1111/1751-7915.70132","DOIUrl":"https://doi.org/10.1111/1751-7915.70132","url":null,"abstract":"Beyond the rational construction of genetic determinants to encode target functions, complex phenotype engineering requires the contextualisation of their expression within the metabolic and genetic background of the host strain. Furthermore, wherever metabolic complexity is involved, phenotype engineering demands standard, reliable, plug-and-play tools. We introduce GENIO (GENome Integration and fitness Optimization platform for Pseudomonas putida), a framework to optimise genetic circuit performance by means of (i) chromosome-location-based differential gene expression and (ii) subsequent fitness improvement through evolutionary engineering if needed. Using gene expression strength and cell-to-cell variation, we characterised 10 P. putida chromosomal loci (ppLPS) to show that genome context rather than distance to ORI is the main factor driving differential expression performance. We further contextualised ppLPS gene expression against well-known chromosomal integration sites and plasmids displaying different copy numbers. GENIO supports comprehensive exploration of the gene expression space across P. putida's genome while unlocking performance optimization of complex heterologous metabolic pathways through evolutionary engineering. To demonstrate the usability of GENIO, we restored P. putida's aromatic hydrocarbon metabolism by (de)constructing the toluene/m-xylene catabolic pathway coded in the pWW0 plasmid. We also showed that engineering complex phenotypes requires accurate contextualisation of the synthetic pathways involved, a process that benefits from biological robustness.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semirational Design of SenC to Enhance Organic Selenium Biosynthesis 促进有机硒生物合成的SenC半设计。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-22 DOI: 10.1111/1751-7915.70130

Kailin Shao, Xiaobin Yu, Yan Zhao, Ying Zhang, Xiaobo Liu

引用次数: 0

Model-Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production 提高微生物采油效率的脂质体耶氏菌模型驱动工程。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-20 DOI: 10.1111/1751-7915.70089

Zeynep Efsun Duman-Özdamar, Mattijs K. Julsing, Vitor A. P. Martins dos Santos, Jeroen Hugenholtz, Maria Suarez-Diez

{"title":"Model-Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production","authors":"Zeynep Efsun Duman-Özdamar, Mattijs K. Julsing, Vitor A. P. Martins dos Santos, Jeroen Hugenholtz, Maria Suarez-Diez","doi":"10.1111/1751-7915.70089","DOIUrl":"10.1111/1751-7915.70089","url":null,"abstract":"Extensive usage of plant-based oils, especially palm oil, has led to environmental and social issues, such as deforestation and loss of biodiversity, thus sustainable alternatives are required. Microbial oils, especially from Yarrowia lipolytica, offer a promising solution because of their similar composition to palm oil, low carbon footprint and ability to utilise low-cost substrates. In this study, we employed the Design-Build-Test-Learn (DBTL) approach to enhance lipid production in Y. lipolytica. We systematically evaluated predictions from the genome-scale metabolic model to identify and overcome bottlenecks in lipid biosynthesis. We tested the effect of predicted medium supplements (glutamate, leucine, methionine and threonine) and genetic intervention targets, including the overexpression of ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACC), threonine synthase (TS), diacylglycerol acyltransferase(DGA1), the deletion of citrate exporter gene (CEX1) and disruption of β-oxidation pathway (MFE1). This work revealed the critical roles of ACC, ACL, TS and DGA1 and the interaction of these genes with elevated intracellular citrate availability in lipid biosynthesis. Combining TS and DGA1 overexpression in the Δmfe_Δcex background achieved a remarkable 200% increase in lipid content (56% w/w) and a 230% increase in lipid yield on glycerol. These findings underscore the potential of Y. lipolytica as an efficient microbial cell factory for fatty acid production. Our study advances the understanding of lipid metabolism in Y. lipolytica and demonstrates a viable approach for developing sustainable and economically feasible alternatives to palm oil.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0