Microbial Biotechnology最新文献

{"title":"Pseudomonas aeruginosa Performs Chemotaxis to All Major Human Neurotransmitters","authors":"Elizabet Monteagudo-Cascales,&nbsp;Miguel A. Matilla,&nbsp;Zulema Udaondo,&nbsp;José A. Gavira,&nbsp;Tino Krell","doi":"10.1111/1751-7915.70211","DOIUrl":"https://doi.org/10.1111/1751-7915.70211","url":null,"abstract":"<p>The ubiquitous pathogen <i>Pseudomonas aeruginosa</i> is attracted to γ-aminobutyrate (GABA), acetylcholine, histamine, serotonin, epinephrine, norepinephrine, dopamine, tyramine, glycine, and glutamate via chemotaxis. These compounds are all major neurotransmitters in humans. They are also found in various non-neuronal tissues and are synthesised by different organisms, including bacteria, protozoa, invertebrates, and plants. Many of these neurotransmitters increase the expression of virulence-related genes in <i>P. aeruginosa</i>, so that chemotaxis to these compounds may constitute an important virulence factor. The chemotactic response is initiated by the direct binding of these compounds to the dCache ligand-binding domains of the PctC, TlpQ, PctD, PctA, and PctB chemoreceptors. Previous studies have shown that <i>Escherichia coli</i> is attracted to epinephrine, norepinephrine, and dopamine. These responses are mediated by the Tar and Tsr chemoreceptors, which possess four-helix bundle-type ligand-binding domains. The use of structurally dissimilar chemoreceptors to mediate neurotransmitter chemotaxis suggests convergent evolution. This article is intended to stimulate the study of the connection between neurotransmitter chemotaxis and virulence in <i>P. aeruginosa</i> and to expand the search for neurotransmitter chemotaxis in other motile bacteria.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of Clinically Important Bacteria With Cold Atmospheric Plasma","authors":"Ruolan Ding,&nbsp;Jiajun Song,&nbsp;Xiaonan Huang,&nbsp;Li Tan,&nbsp;Xiancai Rao,&nbsp;Yi Yang","doi":"10.1111/1751-7915.70219","DOIUrl":"10.1111/1751-7915.70219","url":null,"abstract":"<p>Antimicrobial resistance (AMR), especially in clinically important bacteria, has posed serious challenges to clinical treatments. Novel and effective antimicrobial strategies are urgently needed to address AMR. Cold atmospheric plasma (CAP) is a new concept of disinfection method that kills bacteria through various active species and particles within an ionised and electrical-balanced gas. In this review, we introduced the generation of CAP and summarised its disinfection mechanisms. Moreover, we reviewed the applications of CAP in treating globally important bacteria, including Gram-positive bacteria such as <i>Staphylococcus aureus</i>, <i>Enterococcus</i> spp., <i>Streptococcus pyogenes</i> and <i>Mycobacterium tuberculosis</i>, as well as Gram-negative bacteria including <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i> and <i>Neisseria gonorrhoeae</i>. Additionally, we discussed technological strategies to enhance CAP disinfection efficacy and evaluated the safety of CAP applications. We recommend CAP as an effective alternative technology for combating bacterial infections and hope that the comprehensive information provided in the present review will facilitate the development of CAP-based disinfection strategies to overcome AMR issues in the future.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of Clinically Important Bacteria With Cold Atmospheric Plasma","authors":"Ruolan Ding,&nbsp;Jiajun Song,&nbsp;Xiaonan Huang,&nbsp;Li Tan,&nbsp;Xiancai Rao,&nbsp;Yi Yang","doi":"10.1111/1751-7915.70219","DOIUrl":"10.1111/1751-7915.70219","url":null,"abstract":"<p>Antimicrobial resistance (AMR), especially in clinically important bacteria, has posed serious challenges to clinical treatments. Novel and effective antimicrobial strategies are urgently needed to address AMR. Cold atmospheric plasma (CAP) is a new concept of disinfection method that kills bacteria through various active species and particles within an ionised and electrical-balanced gas. In this review, we introduced the generation of CAP and summarised its disinfection mechanisms. Moreover, we reviewed the applications of CAP in treating globally important bacteria, including Gram-positive bacteria such as <i>Staphylococcus aureus</i>, <i>Enterococcus</i> spp., <i>Streptococcus pyogenes</i> and <i>Mycobacterium tuberculosis</i>, as well as Gram-negative bacteria including <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i> and <i>Neisseria gonorrhoeae</i>. Additionally, we discussed technological strategies to enhance CAP disinfection efficacy and evaluated the safety of CAP applications. We recommend CAP as an effective alternative technology for combating bacterial infections and hope that the comprehensive information provided in the present review will facilitate the development of CAP-based disinfection strategies to overcome AMR issues in the future.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Strategies to Overcome Fungal Virulence in Crop Protection","authors":"C. A. Molina-Santiago,&nbsp;D. Vela-Corcía","doi":"10.1111/1751-7915.70220","DOIUrl":"https://doi.org/10.1111/1751-7915.70220","url":null,"abstract":"<p>Fungal pathogens are major threats to global crop production, intensified by rising fungicide resistance and the limited availability of resistant cultivars. This highlight article outlines recent molecular strategies aimed at reducing fungal virulence through sustainable and targeted approaches. RNA interference (RNAi) has emerged as a precise method to silence essential genes in pathogens, significantly impairing virulence and development. In parallel, inhibiting fungal efflux transporters—particularly ABC and MFS proteins—has been shown to reverse multidrug resistance and restore fungicide efficacy in pathogens like <i>Botrytis cinerea</i>. Additionally, engineering biocontrol agents expressing anti-apoptotic genes enhances their growth, stress resistance, and mycoparasitic activity. These strategies collectively illustrate the potential of combining RNAi technologies, efflux inhibition, and genetically enhanced biocontrol agents to create integrated, environmentally friendly plant protection systems. Such precision-targeted approaches represent a promising alternative to traditional chemical control, aligning with global efforts to achieve sustainable agriculture.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70220","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Critical Environmental Optima and Biotechnological Prospects of Fungal Fruiting Bodies","authors":"Amechi S. Nwankwegu,&nbsp;Sinang Hongsanan,&nbsp;Uzoma P. Nwankwegu,&nbsp;Ning Xie","doi":"10.1111/1751-7915.70210","DOIUrl":"10.1111/1751-7915.70210","url":null,"abstract":"<p>Fruiting body development is a principal mechanism in fungal morphogenesis, which often involves complex interplays of hormonal regulation, gene expression, and metabolic immobilisation influenced by environmental interactions, ultimately leading to the differentiation of multicellular structures. In fungal communities, including ascomycetes and basidiomycetes, fruiting body development ensures protection and facilitates the dispersal of ascospores. Constrained by environmental factors that vary across morphogenetic stages, a thorough synthesis of the critical ecological optima, which primarily regulate the multi-omics footprint encompassing diverse molecular perspectives characterising fruiting body formations, is key. It underscores that exceeding the critical environmental ranges triggers dynamic shifts that adversely impact the fruiting body's eco-resilience; however, operating below these optima is safer, as most fruiting body physiological activities are generally able to maintain normal functioning and stability, making the present study relevant to decision-makers for optimal fruiting body commercialisation. It elucidates the recent advances in fruiting body biotechnologies, traversing agricultural/food, optimised cultivation strategies, environmental and health, bioactive compounds extractions, genetic engineering, and synthetic biology, promoting scalable bioproduction. Nonetheless, it proposes further studies emphasising omics-driven strain-substrate improvements/genomic modifications, incorporating CRISPR advances, to boost precision cultivation and enhance robust strain design. The study offers promising insights into complementing existing knowledge on fungal fruiting bodies and addresses challenges related to environmental complexity and uncertainties, aiming to drive sustainable industrial biotechnology.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Critical Environmental Optima and Biotechnological Prospects of Fungal Fruiting Bodies","authors":"Amechi S. Nwankwegu,&nbsp;Sinang Hongsanan,&nbsp;Uzoma P. Nwankwegu,&nbsp;Ning Xie","doi":"10.1111/1751-7915.70210","DOIUrl":"10.1111/1751-7915.70210","url":null,"abstract":"<p>Fruiting body development is a principal mechanism in fungal morphogenesis, which often involves complex interplays of hormonal regulation, gene expression, and metabolic immobilisation influenced by environmental interactions, ultimately leading to the differentiation of multicellular structures. In fungal communities, including ascomycetes and basidiomycetes, fruiting body development ensures protection and facilitates the dispersal of ascospores. Constrained by environmental factors that vary across morphogenetic stages, a thorough synthesis of the critical ecological optima, which primarily regulate the multi-omics footprint encompassing diverse molecular perspectives characterising fruiting body formations, is key. It underscores that exceeding the critical environmental ranges triggers dynamic shifts that adversely impact the fruiting body's eco-resilience; however, operating below these optima is safer, as most fruiting body physiological activities are generally able to maintain normal functioning and stability, making the present study relevant to decision-makers for optimal fruiting body commercialisation. It elucidates the recent advances in fruiting body biotechnologies, traversing agricultural/food, optimised cultivation strategies, environmental and health, bioactive compounds extractions, genetic engineering, and synthetic biology, promoting scalable bioproduction. Nonetheless, it proposes further studies emphasising omics-driven strain-substrate improvements/genomic modifications, incorporating CRISPR advances, to boost precision cultivation and enhance robust strain design. The study offers promising insights into complementing existing knowledge on fungal fruiting bodies and addresses challenges related to environmental complexity and uncertainties, aiming to drive sustainable industrial biotechnology.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Strategies to Overcome Fungal Virulence in Crop Protection","authors":"C. A. Molina-Santiago,&nbsp;D. Vela-Corcía","doi":"10.1111/1751-7915.70220","DOIUrl":"10.1111/1751-7915.70220","url":null,"abstract":"<p>Fungal pathogens are major threats to global crop production, intensified by rising fungicide resistance and the limited availability of resistant cultivars. This highlight article outlines recent molecular strategies aimed at reducing fungal virulence through sustainable and targeted approaches. RNA interference (RNAi) has emerged as a precise method to silence essential genes in pathogens, significantly impairing virulence and development. In parallel, inhibiting fungal efflux transporters—particularly ABC and MFS proteins—has been shown to reverse multidrug resistance and restore fungicide efficacy in pathogens like <i>Botrytis cinerea</i>. Additionally, engineering biocontrol agents expressing anti-apoptotic genes enhances their growth, stress resistance, and mycoparasitic activity. These strategies collectively illustrate the potential of combining RNAi technologies, efflux inhibition, and genetically enhanced biocontrol agents to create integrated, environmentally friendly plant protection systems. Such precision-targeted approaches represent a promising alternative to traditional chemical control, aligning with global efforts to achieve sustainable agriculture.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70220","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuous Spectrophotometric Assay for Defluorinase and Dechlorinase Activities With α-Halocarboxylic Acids","authors":"Marie Ronnander,&nbsp;Anthony G. Dodge,&nbsp;Erin O'Neal,&nbsp;Caroline Pauls,&nbsp;Jack Hanson,&nbsp;James K. Christenson,&nbsp;Lawrence P. Wackett","doi":"10.1111/1751-7915.70216","DOIUrl":"10.1111/1751-7915.70216","url":null,"abstract":"<p>Many environmental pollutants have a fluorine or chlorine atom on a carbon atom adjacent to a carboxylic acid. These α-halocarboxylic acids include heavily regulated compounds such as per- and polyfluorinated substances (PFAS). Due to PFAS persistence in the environment, there is intense interest in characterising the biodegradation of α-halocarboxylic acids. Their initial biodegradation often proceeds via defluorinase enzymes that catalyse hydrolytic removal of <i>alpha</i> fluorine or chlorine atoms. These enzymes can dehalogenate both mono-halocarboxylate and dihalocarboxylate substrates, generating α-hydroxy and α-ketocarboxylic acid products, respectively. To enable continuous monitoring of defluorinase activity, we identified, purified and optimised dehydrogenases from <i>Limosilactobacillus fermentum</i> JN248 and <i>Enterococcus faecium</i> IAM10071 that reacted with the specific α-hydroxy and α-ketocarboxylic acid products of the defluorinases. The dehydrogenases make or consume NADH, measured by absorbance readings at 340 nm, thus allowing continuous measurement of defluorinase activity using a spectrophotometer. Using the coupled assay, purified defluorinases from a <i>Delftia</i> sp. and a <i>Dechloromonas</i> sp. were compared with respect to substrate specificity. The <i>Delftia</i> defluorinase demonstrated superior activity with most substrates, including difluoroacetate. To our knowledge, this is the first report of a coupled-enzyme continuous assay method for enzymes that catalyse the hydrolysis of α-halocarboxylic acids.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuous Spectrophotometric Assay for Defluorinase and Dechlorinase Activities With α-Halocarboxylic Acids","authors":"Marie Ronnander,&nbsp;Anthony G. Dodge,&nbsp;Erin O'Neal,&nbsp;Caroline Pauls,&nbsp;Jack Hanson,&nbsp;James K. Christenson,&nbsp;Lawrence P. Wackett","doi":"10.1111/1751-7915.70216","DOIUrl":"10.1111/1751-7915.70216","url":null,"abstract":"<p>Many environmental pollutants have a fluorine or chlorine atom on a carbon atom adjacent to a carboxylic acid. These α-halocarboxylic acids include heavily regulated compounds such as per- and polyfluorinated substances (PFAS). Due to PFAS persistence in the environment, there is intense interest in characterising the biodegradation of α-halocarboxylic acids. Their initial biodegradation often proceeds via defluorinase enzymes that catalyse hydrolytic removal of <i>alpha</i> fluorine or chlorine atoms. These enzymes can dehalogenate both mono-halocarboxylate and dihalocarboxylate substrates, generating α-hydroxy and α-ketocarboxylic acid products, respectively. To enable continuous monitoring of defluorinase activity, we identified, purified and optimised dehydrogenases from <i>Limosilactobacillus fermentum</i> JN248 and <i>Enterococcus faecium</i> IAM10071 that reacted with the specific α-hydroxy and α-ketocarboxylic acid products of the defluorinases. The dehydrogenases make or consume NADH, measured by absorbance readings at 340 nm, thus allowing continuous measurement of defluorinase activity using a spectrophotometer. Using the coupled assay, purified defluorinases from a <i>Delftia</i> sp. and a <i>Dechloromonas</i> sp. were compared with respect to substrate specificity. The <i>Delftia</i> defluorinase demonstrated superior activity with most substrates, including difluoroacetate. To our knowledge, this is the first report of a coupled-enzyme continuous assay method for enzymes that catalyse the hydrolysis of α-halocarboxylic acids.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to ‘Metagenomic Exploration Uncovers Several Novel “Candidatus” Species Involved in Acetate Metabolism in High-Ammonia Thermophilic Biogas Processes’","authors":"","doi":"10.1111/1751-7915.70217","DOIUrl":"10.1111/1751-7915.70217","url":null,"abstract":"&lt;p&gt;Cheng, G. B., E. Bongcam-Rudloff, and A. Schnürer. 2025. \"Metagenomic Exploration Uncovers Several Novel ‘&lt;i&gt;Candidatus&lt;/i&gt;’ Species Involved in Acetate Metabolism in High-Ammonia Thermophilic Biogas Processes.\" &lt;i&gt;Microbial Biotechnology&lt;/i&gt; 18: e70133. https://doi.org/10.1111/1751-7915.70133.&lt;/p&gt;&lt;p&gt;In our original publication, we mistakenly acknowledged Prof. Oren for assisting with the naming of the candidate species. While we did discuss general nomenclatural principles with Prof. Oren, the specific names proposed in the original version were developed independently by the authors, and he did not advise on or approve those names. We regret this misrepresentation and in this corrigendum, we present revised naming suggestions that better reflect appropriate taxonomic practices. These revisions have benefited from input by Prof Oren, whose guidance on naming conventions and principles we gratefully acknowledge.&lt;/p&gt;&lt;p&gt;We here present the corrected versions of the protologues for the four new &lt;i&gt;Candidatus&lt;/i&gt; genera and species proposed in Section 3.7 of the original paper. ‘&lt;i&gt;Candidatus&lt;/i&gt; Thermotepidanaerobacter aceticum’ have been changed to ‘&lt;i&gt;Candidatus&lt;/i&gt; Thermotepidanaerobacter aceticus’ and ‘&lt;i&gt;Candidatus&lt;/i&gt; Thermodarwinisyntropha acetovorans’ have been changed to‘&lt;i&gt;Candidatus&lt;/i&gt; Thermodarwinisyntropha acetivorans’. Numbers of figures and tables and references cited below refer to that paper. Further information about the taxa can be found in the original publication and its supporting information. In Figure 3, the updated taxonomic names are included in this corrigendum. Furthermore, there was a spelling mistake of the phylum &lt;i&gt;Thermotogota&lt;/i&gt;. It was previously spelled as &lt;i&gt;Thermotoga&lt;/i&gt;, which is the genus name. Moreover, the original name of &lt;i&gt;Metanoculleus thermohydrogenotrophicum&lt;/i&gt; was given in the databases (GTDB/NCBI), while the name later has been updated to‘&lt;i&gt;Candidatus&lt;/i&gt; Methanoculleus thermohydrogenitrophicus’. Figure 3 has been updated accordingly.&lt;/p&gt;&lt;p&gt;We apologize for this error.&lt;/p&gt;&lt;p&gt;The corrected Section 3.7 should read as:&lt;/p&gt;&lt;p&gt;\u0000 &lt;b&gt;3.7 Description of New Genus/Species&lt;/b&gt;\u0000 &lt;/p&gt;&lt;p&gt;The genomes presented below are novel species with the ability to produce or consume acetate via WLP/GSRP, which motivated a further analysis to reveal information on their genomic potential and classification as ‘Candidatus’ species. The analysis included both an investigation of general genome characteristics and phylogeny as well as analysis involving carbon and energy metabolism (Table S4).&lt;/p&gt;&lt;p&gt;&lt;b&gt;‘&lt;i&gt;Candidatus&lt;/i&gt; Thermotepidanaerobacter’ gen. nov&lt;/b&gt;.&lt;/p&gt;&lt;p&gt;Thermotepidanaerobacter (Ther.mo.te.pid.an.ae.ro.bac'ter. Gr. masc. adj. &lt;i&gt;thermos&lt;/i&gt;, hot; N.L. masc. n. &lt;i&gt;Tepidanaerobacter&lt;/i&gt;, a bacterial genus name; N.L. masc. n. ‘&lt;i&gt;Candidatus&lt;/i&gt; Thermotepidanaerobacter’, a hot-loving &lt;i&gt;Tepidanaerobacter&lt;/i&gt;).&lt;/p&gt;&lt;p&gt;The properties of the genus are as the properties of the only species describ","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}