Microbial Biotechnology最新文献_第6页

De Novo Biosynthesis of Antidepressant Psilocybin in Escherichia coli 抗抑郁药裸盖菇素在大肠杆菌中的新生生物合成

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-03 DOI: 10.1111/1751-7915.70135

Zhangrao Huang, Yongpeng Yao, Rouyu Di, JianChao Zhang, Yuanyuan Pan, Gang Liu

{"title":"De Novo Biosynthesis of Antidepressant Psilocybin in Escherichia coli","authors":"Zhangrao Huang, Yongpeng Yao, Rouyu Di, JianChao Zhang, Yuanyuan Pan, Gang Liu","doi":"10.1111/1751-7915.70135","DOIUrl":"https://doi.org/10.1111/1751-7915.70135","url":null,"abstract":"Psilocybin, a tryptamine-derived alkaloid, has been granted Breakthrough Therapy designation by the U.S. FDA for treatment-resistant depression, underscoring its clinical importance. Therefore, sustainable and economic production is urgently needed. Manufacturing of psilocybin in Escherichia coli has drawn great attention. However, due to the low expression and activity of the eukaryotic cytochrome P450 enzyme PsiH in the psilocybin biosynthetic pathway, de novo synthesis of psilocybin in prokaryotic cells has been hampered. To overcome this dilemma, we herein demonstrated de novo synthesis of psilocybin in E. coli by constructing PsiH variants with N-terminal domain modifications and expressing the entire biosynthetic pathway at a concordantly low temperature. Improving the supply of precursor and engineering the P450 electron transfer chain resulted in a 33-fold increase in the titre of norbaeocystin (105.3 mg/L), a key intermediate of psilocybin biosynthesis, and a 17-fold increase in the titre of psilocybin (14 mg/L). Further enhancement of psilocybin production was achieved by converting norbaeocystin to psilocybin by overexpressing an extra copy of the methyltransferase gene psiM. Finally, 79.4 mg/L of psilocybin was produced by optimising flask fermentation conditions, a 100-fold improvement over the starting strain. Our work demonstrates the successful fungal P450 engineering to improve the catalytic activity in E. coli and will advance the sustainable production of the important antidepressant psilocybin in prokaryotic microbial cells.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation of Meat Quality and Relationship to Gut Microbiota Among Different Pig Breeds 不同猪种肉质差异及其与肠道菌群的关系

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-03 DOI: 10.1111/1751-7915.70139

Qifan Zhang, Man Du, Siyu Wei, Luoyi Zhu, Rong Yan, Mingliang Jin, Yizhen Wang

{"title":"Variation of Meat Quality and Relationship to Gut Microbiota Among Different Pig Breeds","authors":"Qifan Zhang, Man Du, Siyu Wei, Luoyi Zhu, Rong Yan, Mingliang Jin, Yizhen Wang","doi":"10.1111/1751-7915.70139","DOIUrl":"https://doi.org/10.1111/1751-7915.70139","url":null,"abstract":"Meat production is of great importance to the world's food supply and economic development, and meat quality determines the purchasing desire of consumers. Recent studies show intestinal microorganisms are involved in several physiological functions of the host and therefore are likely to regulate meat quality. This study aimed to compare the carcass performance, meat quality traits and serum parameters of three different pig breeds, Jinhua (JH) pigs (n = 8), Duroc × Berkshire × Jiaxinghei (DBJ) pigs (n = 8), Duroc × Landrace × Yorkshire (DLY) pigs (n = 8) and to investigate a possible relationship between gut microbiota composition and these traits. Meat quality results showed that compared with DLY pigs, JH pigs had lower water loss and shear force in the longissimus dorsi muscle, and higher intramuscular fat content and inosine monophosphate content were observed in JH pigs. Serum biochemical indicators showed the content of nonesterified fatty acid in JH pigs was lowest. Furthermore, the gut microbiota analysis indicated that JH pigs harboured more abundant Lachnospiraceae, Prevotellaceae NK3B31 and Marvinbryantia. Spearman correlation analysis showed that unidentified genus of family Lachnospiraceae, genus Prevotella and genus Alloprevotella were positively correlated with IMF content and marbling score in the longissimus dorsi muscle of pigs. In conclusion, our results indicated the quality of JH pork was superior to DBJ and DLY pigs, and the difference in meat qualities was related to the abundance of fibre-degrading bacteria. Our study provides insight into further understanding of the relationship between microbiota and meat quality, nutrient metabolism and fat deposition, which is critical to the pork industry and swine intestinal health.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the Glycan Code: Molecular Dynamics and Quantum Chemistry Reveal How O-Glycan Functional Groups Govern OgpA Selectivity in Mucin Degradation by Akkermansia muciniphila 揭开聚糖密码：分子动力学和量子化学揭示了o -聚糖官能团如何控制嗜粘蛋白降解中OgpA的选择性

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-03 DOI: 10.1111/1751-7915.70091

Mohammad Khavani, Aliyeh Mehranfar, Mohammad R. K. Mofrad

{"title":"Unravelling the Glycan Code: Molecular Dynamics and Quantum Chemistry Reveal How O-Glycan Functional Groups Govern OgpA Selectivity in Mucin Degradation by Akkermansia muciniphila","authors":"Mohammad Khavani, Aliyeh Mehranfar, Mohammad R. K. Mofrad","doi":"10.1111/1751-7915.70091","DOIUrl":"https://doi.org/10.1111/1751-7915.70091","url":null,"abstract":"Mucins, heavily O-glycosylated glycoproteins, are a key component of mucus, and certain gut microbiota, including Akkermansia muciniphila, can utilise mucin glycans as a carbon source. Akkermansia muciniphila produces the O-glycopeptidase enzyme OgpA, which cleaves peptide bonds at the N-terminus of serine (Ser) or threonine (Thr) residues carrying O-glycan substitutions, with selectivity influenced by the O-glycan functional groups. Using molecular dynamics (MD) simulations and quantum chemistry calculations, we explored how different O-glycan groups affect OgpA's selectivity. Our results show that peptides bind to the enzyme via hydrogen bonds, π–π interactions, van der Waals forces and electrostatic interactions, with key residues, including Tyr90, Val138, Gly176, Tyr210 and Glu91, playing important roles. The primary determinant of selectivity is the interaction between the peptide's functional group and the enzyme's binding cavity, while peptide–enzyme interface interactions are secondary. Quantum chemistry calculations reveal that OgpA prefers peptides with a lower electrophilic character. This study provides new insights into mucin degradation by gut microbiota enzymes, advancing our understanding of this critical biological process.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Dissection of Cyclic di-GMP Signalling in Pseudomonas aeruginosa via Systematic Diguanylate Cyclase Disruption 铜绿假单胞菌系统双胍酸环化酶破坏对环二gmp信号的遗传剖析

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-02 DOI: 10.1111/1751-7915.70137

Román A. Martino, Daniel C. Volke, Albano H. Tenaglia, Paula M. Tribelli, Pablo I. Nikel, Andrea M. Smania

{"title":"Genetic Dissection of Cyclic di-GMP Signalling in Pseudomonas aeruginosa via Systematic Diguanylate Cyclase Disruption","authors":"Román A. Martino, Daniel C. Volke, Albano H. Tenaglia, Paula M. Tribelli, Pablo I. Nikel, Andrea M. Smania","doi":"10.1111/1751-7915.70137","DOIUrl":"https://doi.org/10.1111/1751-7915.70137","url":null,"abstract":"The second messenger bis-(3′ → 5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) governs adaptive responses in the opportunistic pathogen Pseudomonas aeruginosa, including biofilm formation and the transition from acute to chronic infections. Understanding the intricate c-di-GMP signalling network remains challenging due to the overlapping activities of numerous diguanylate cyclases (DGCs). In this study, we employed a CRISPR-based multiplex genome-editing tool to disrupt all 32 GGDEF domain-containing proteins (GCPs) implicated in c-di-GMP signalling in P. aeruginosa PA14. Phenotypic and physiological analyses revealed that the resulting mutant was unable to form biofilms and had attenuated virulence. Residual c-di-GMP levels were still detected despite the extensive GCP disruption, underscoring the robustness of this regulatory network. Taken together, these findings provide insights into the complex c-di-GMP metabolism and showcase the importance of functional overlapping in bacterial signalling. Moreover, our approach overcomes the native redundancy in c-di-GMP synthesis, providing a framework to dissect individual DGC functions and paving the way for targeted strategies to address bacterial adaptation and pathogenesis.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Single-Plasmid Inducible-Replication System for High-Yield Production of Short Ff (f1, M13 or fd)-Phage-Derived Nanorods 单质粒诱导复制系统高产短Ff （f1， M13或fd）噬菌体衍生纳米棒

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-01 DOI: 10.1111/1751-7915.70113

Rayén Ignacia León-Quezada, Majela González Miró, Sofia Khanum, Andrew J. Sutherland-Smith, Vicki A. M. Gold, Jasna Rakonjac

{"title":"A Single-Plasmid Inducible-Replication System for High-Yield Production of Short Ff (f1, M13 or fd)-Phage-Derived Nanorods","authors":"Rayén Ignacia León-Quezada, Majela González Miró, Sofia Khanum, Andrew J. Sutherland-Smith, Vicki A. M. Gold, Jasna Rakonjac","doi":"10.1111/1751-7915.70113","DOIUrl":"https://doi.org/10.1111/1751-7915.70113","url":null,"abstract":"Ff (f1, M13 or fd) filamentous phages have been used for myriad applications including phage display, assembly of nanostructures and as carriers of agents used for diagnostic and therapeutic purposes. Recently, short Ff phage-derived functionalised nanorods have emerged as a superior alternative to full-length filamentous phages for applications from lateral flow assays to cell- and tissue-targeting. Their advantages, such as shorter length and the lack of antibiotic resistance genes, make them particularly promising for expanding the current scope of Ff bionanotechnology and biomedical applications. Limitations to the widespread use of Ff-derived nanorods include a requirement for two plasmids and the relatively low production efficiency. This is due to the presence of only the positive Ff origin of replication, allowing replication of only the positive strand. Here we describe a single-plasmid negative origin-containing inducible-replication system for nanorod production. These improvements simplify and increase nanorod production by two orders of magnitude compared with the constitutive positive origin-only production system. The high concentration of nanorods allows formation of higher-order structures, such as stacks and rafts, as imaged by transmission electron microscopy. In summary, our system will facilitate production and expand the applications of Ff-derived biological nanorods.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome-Based Synthetic Protein Scaffold 设计糖酵解体：糖酵解酶在纤维素基合成蛋白支架上的共定位

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-31 DOI: 10.1111/1751-7915.70134

Marte Elias, Kenan Meert, Julie Vanderstraeten, Babette Lamote, Yves Briers

{"title":"Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome-Based Synthetic Protein Scaffold","authors":"Marte Elias, Kenan Meert, Julie Vanderstraeten, Babette Lamote, Yves Briers","doi":"10.1111/1751-7915.70134","DOIUrl":"https://doi.org/10.1111/1751-7915.70134","url":null,"abstract":"In systems biocatalysis, combining pathway enzymes in vitro allows for the conversion of basic substrates into more complex, valuable chemicals. However, in vitro enzyme cascades are not yet economically viable for large-scale bio-based chemical production. Enhancing pathway efficiency through enzyme colocalization on synthetic protein scaffolds is a proposed solution, though still debated. We constructed a synthetic protein scaffold that colocalises the first three glycolytic enzymes using cohesin–dockerin interactions. Initially, we converted wild-type enzymes to the docking enzyme mode and evaluated their activity. Next, we demonstrate how the colocalisation of the three docking enzymes on distinct scaffolds enhances the enzyme cascade's production. Starting from glucose, the multi-enzyme complexes produced fructose-1,6-bisphosphate, confirming the activity of each enzyme. PfkA, which converts fructose-6-phosphate and ATP to fructose-1,6-bisphosphate and ADP, was identified as the rate-limiting enzyme. We demonstrated that scaffolding proximity effects lead to higher product output than free docking enzymes, particularly at lower enzyme densities. Further research is needed to determine the relevance of enzyme colocalisation under industrial production settings. In addition, optimising an enzyme cascade demands a thorough understanding of reaction mechanisms and kinetics. The VersaTile method streamlines optimisation studies of modular proteins and complexes, enabling analysis of a broader design space by bypassing technical preparatory hurdles.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Novel Gene Clusters for Secondary Metabolite Synthesis in Mesophotic Sponge-Associated Bacteria” 更正“介孔海绵相关细菌次级代谢物合成的新基因簇”

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-26 DOI: 10.1111/1751-7915.70136

引用次数: 0

Correction to “Novel Gene Clusters for Secondary Metabolite Synthesis in Mesophotic Sponge-Associated Bacteria” 更正“介孔海绵相关细菌次级代谢物合成的新基因簇”

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-26 DOI: 10.1111/1751-7915.70136

引用次数: 0

Correction to ‘Multiple Chaperone DnaK–FliC Flagellin Interactions Are Required for Pseudomonas aeruginosa Flagellum Assembly and Indicate a New Function for DnaK’ 更正“铜绿假单胞菌鞭毛组装需要多个伴侣dna - flc鞭毛蛋白相互作用，并表明DnaK的新功能”

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-26 DOI: 10.1111/1751-7915.70138

{"title":"Correction to ‘Multiple Chaperone DnaK–FliC Flagellin Interactions Are Required for Pseudomonas aeruginosa Flagellum Assembly and Indicate a New Function for DnaK’","authors":"","doi":"10.1111/1751-7915.70138","DOIUrl":"https://doi.org/10.1111/1751-7915.70138","url":null,"abstract":"Molinari, G., S. S. Ribeiro, K. Müller, et al. 2025. “Multiple Chaperone DnaK–FliC Flagellin Interactions Are Required for Pseudomonas aeruginosa Flagellum Assembly and Indicate a New Function for DnaK.” Microbial Biotechnology 18, no. 2: e70096. https://doi.org/10.1111/1751-7915.70096.In paragraph 4 of the ‘3.8 | DnaK Functions in an ATP-Independent Manner at Both Physiological and Mild Acidic pHs’ subsection in the ‘Results’ section, the equation ‘ΔD/AInitial = D/AiDnaKD − AiSOD1’ was incorrect. This should have read: ‘ΔD/AInitial = D/AiDnaK − D/AiSOD1’.In caption of Figure 6 (C) of the ‘Results’ section, the equation ‘ΔD/AInitial = D/AiDnaK/NucleotidesD/AiSOD1’ was incorrect. This should have read: ‘ΔD/AInitial = D/AiDnaK/Nucleotides − D/AiSOD1’.We apologise for this error.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecology and Evolutionary Biology as Frameworks to Study Wine Fermentations 生态学和进化生物学作为研究葡萄酒发酵的框架

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-26 DOI: 10.1111/1751-7915.70078

Ignacio Belda, Belen Benitez-Dominguez, Sergio Izquierdo-Gea, Jean C. C. Vila, Javier Ruiz

引用次数: 0