Microbial Biotechnology最新文献_第6页

Enabling Access to Novel Bacterial Biosynthetic Potential From ONT Draft Genomic Data

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-04 DOI: 10.1111/1751-7915.70104

Marco A. Campos-Magaña, Vitor A. P. Martins dos Santos, Luis Garcia-Morales

{"title":"Enabling Access to Novel Bacterial Biosynthetic Potential From ONT Draft Genomic Data","authors":"Marco A. Campos-Magaña, Vitor A. P. Martins dos Santos, Luis Garcia-Morales","doi":"10.1111/1751-7915.70104","DOIUrl":"https://doi.org/10.1111/1751-7915.70104","url":null,"abstract":"Natural products comprise a wide diversity of compounds with a range of biological activities, including antibiotics, anti-inflammatory and anti-tumoral molecules. However, we can only access a small portion of these compounds due to various technical difficulties. We have herein developed a novel and efficient approach for accessing biosynthetic gene clusters (BGCs) that encode natural products from soil bacteria. The pipeline uses a combination of long-read sequencing, antiSMASH for BGC identification and Transformation-associated recombination (TAR) for cloning the BGCs. We hypothesized that a genome assembly using Oxford Nanopore Technology (ONT) sequencing could facilitate the detection of large BGCs at a relatively fast and low-cost DNA sequencing. Despite the relative low accuracy and sequence mistakes due to high GC content and sequence repetitions frequently found in BGC containing bacteria, we demonstrate that ONT long-read sequencing and antiSMASH are effective for identifying novel BGCs and enabling TAR cloning to isolate the BGC in a desired vector. We applied this pipeline on a previously non-sequenced myxobacteria Aetherobacter fasciculatus SBSr002. Our approach enabled us to clone a previously unknown BGC into a genome engineering-ready vector, illustrating the capabilities of this powerful and cost-effective strategy.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When Science Meets Creativity: Elevating Microbiology Education With Art—Two Personal Experiences

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-02 DOI: 10.1111/1751-7915.70099

Luis Andrés Yarzábal Rodríguez, Ramón Alberto Batista-García

{"title":"When Science Meets Creativity: Elevating Microbiology Education With Art—Two Personal Experiences","authors":"Luis Andrés Yarzábal Rodríguez, Ramón Alberto Batista-García","doi":"10.1111/1751-7915.70099","DOIUrl":"https://doi.org/10.1111/1751-7915.70099","url":null,"abstract":"Science communicators are more critical than ever in a time when misinformation and hoaxes dominate social media, especially during global emergencies like the COVID-19 outbreak. Researchers are good at communicating with peers but often struggle to explain complex ideas to the public. As shown previously, expanding outreach by combining science and art is not only possible, but effective. In the case of microbiology, the synergy between these two apparently divorced areas can help educating our communities and raising microbiological awareness, especially among students. Since Alexander Fleming's pioneering attempts, microorganisms have fascinated artists. Art may help us demystifying microbes and making them more approachable, improving public involvement with science. This article presents two personal experiences using art to teach and communicate scientific ideas (mainly from the microbiology field) to our fellow citizens. Above all, we emphasise the importance of contributing to the scientific literacy of our societies at all ages—including underrepresented and disadvantaged groups—by exploring novel ways to address this gap. By doing so, we align with the concepts and aims of the International Microbiology Literacy Initiative (IMiLI).","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Transcriptional Regulator DhyR Positively Modulates Daptomycin Biosynthesis in Streptomyces roseosporus

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-02 DOI: 10.1111/1751-7915.70110

Fuqiang He, Xinpeng Liu, Haiyi Wang, Xu Li, Yun Wu, Dan Zhang, Shufang Liang

{"title":"The Transcriptional Regulator DhyR Positively Modulates Daptomycin Biosynthesis in Streptomyces roseosporus","authors":"Fuqiang He, Xinpeng Liu, Haiyi Wang, Xu Li, Yun Wu, Dan Zhang, Shufang Liang","doi":"10.1111/1751-7915.70110","DOIUrl":"https://doi.org/10.1111/1751-7915.70110","url":null,"abstract":"Daptomycin (DAP) is a cyclic lipopeptide antibiotic produced by Streptomyces roseosporus, and its biosynthesis is precisely regulated by a complex regulatory network. Although the biosynthetic pathway of DAP has been elucidated, the regulatory mechanism governing its biosynthesis at the transcriptional level is not yet fully understood. In the present study, a new transcriptional regulator, DhyR, was identified. A deletion mutant of dhyR was constructed using the CRISPR-Cas9 tool to elucidate the biological role of DhyR thanks to functional and transcriptomic analyses. The results demonstrated that DhyR positively regulates DAP biosynthesis in S. roseosporus. The in-frame deletion of the dhyR gene resulted in a significant downregulation of the transcription levels of all structural genes within the DAP biosynthetic gene cluster and a significant decrease in DAP yield. In contrast, overexpression of dhyR enhanced the transcription levels of the DAP biosynthetic gene cluster, leading to a 23% increase in DAP yield. Deletion of dhyR caused significant changes in the expression of multiple genes involved in carbohydrate metabolism, energy metabolism and amino acid metabolic pathways through transcriptome analysis. Especially, deletion of dhyR led to a significant downregulation of transcription levels of three DAP biosynthesis-associated genes, including atrA, depR1 and ssig-05090. In summary, DhyR positively regulates DAP biosynthesis in S. roseosporus by influencing the expression of the DAP gene cluster and modulating precursor flux. It functions as a pleiotropic regulator of primary and secondary metabolism in S. roseosporus.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-02 DOI: 10.1111/1751-7915.70112

Pedro José Reyes-Pérez, Irene Jiménez-Guerrero, Ana Sánchez-Reina, Cristina Civantos, Natalia Moreno-de Castro, Francisco Javier Ollero, Jacinto Gandullo, Patricia Bernal, Francisco Pérez-Montaño

{"title":"The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin","authors":"Pedro José Reyes-Pérez, Irene Jiménez-Guerrero, Ana Sánchez-Reina, Cristina Civantos, Natalia Moreno-de Castro, Francisco Javier Ollero, Jacinto Gandullo, Patricia Bernal, Francisco Pérez-Montaño","doi":"10.1111/1751-7915.70112","DOIUrl":"https://doi.org/10.1111/1751-7915.70112","url":null,"abstract":"The symbiotic relationship between rhizobia and legumes is critical for sustainable agriculture and has important economic and environmental implications. In this intricate process, rhizobial bacteria colonise plant roots and induce the formation of specialised plant organs, the nodules. Within these structures, rhizobia fix environmental nitrogen into ammonia, significantly reducing the demand for synthetic fertilisers. Multiple bacterial secretion systems (TXSS, Type X Secretion System) are involved in establishing this symbiosis, with T3SS being the most studied. While the Type 6 Secretion System (T6SS) is known as a “nanoweapon” commonly used by diderm (formerly gram-negative) bacteria for inter-bacterial competition and potentially manipulating eukaryotic cells, its precise role in legume symbiosis remains unclear. Sinorhizobium fredii USDA257, a fast-growing rhizobial strain capable of nodulating diverse legume plants, possesses a single T6SS cluster containing genes encoding structural components and potential effectors that could target plant cells and/or act as effector-immunity pairs. Our research reveals that this T6SS can be induced in nutrient-limited conditions and, more importantly, is essential for successful nodulation and competitive colonisation of Glycine max cv Pekin. Although the system did not demonstrate effectiveness in eliminating competing bacteria in vitro, its active presence within root nodules suggests a sophisticated role in symbiotic interactions that extends beyond traditional interbacterial competition.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When Science Meets Creativity: Elevating Microbiology Education With Art—Two Personal Experiences

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-02 DOI: 10.1111/1751-7915.70099

Luis Andrés Yarzábal Rodríguez, Ramón Alberto Batista-García

{"title":"When Science Meets Creativity: Elevating Microbiology Education With Art—Two Personal Experiences","authors":"Luis Andrés Yarzábal Rodríguez, Ramón Alberto Batista-García","doi":"10.1111/1751-7915.70099","DOIUrl":"https://doi.org/10.1111/1751-7915.70099","url":null,"abstract":"Science communicators are more critical than ever in a time when misinformation and hoaxes dominate social media, especially during global emergencies like the COVID-19 outbreak. Researchers are good at communicating with peers but often struggle to explain complex ideas to the public. As shown previously, expanding outreach by combining science and art is not only possible, but effective. In the case of microbiology, the synergy between these two apparently divorced areas can help educating our communities and raising microbiological awareness, especially among students. Since Alexander Fleming's pioneering attempts, microorganisms have fascinated artists. Art may help us demystifying microbes and making them more approachable, improving public involvement with science. This article presents two personal experiences using art to teach and communicate scientific ideas (mainly from the microbiology field) to our fellow citizens. Above all, we emphasise the importance of contributing to the scientific literacy of our societies at all ages—including underrepresented and disadvantaged groups—by exploring novel ways to address this gap. By doing so, we align with the concepts and aims of the International Microbiology Literacy Initiative (IMiLI).","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Transcriptional Regulator DhyR Positively Modulates Daptomycin Biosynthesis in Streptomyces roseosporus

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-02 DOI: 10.1111/1751-7915.70110

Fuqiang He, Xinpeng Liu, Haiyi Wang, Xu Li, Yun Wu, Dan Zhang, Shufang Liang

{"title":"The Transcriptional Regulator DhyR Positively Modulates Daptomycin Biosynthesis in Streptomyces roseosporus","authors":"Fuqiang He, Xinpeng Liu, Haiyi Wang, Xu Li, Yun Wu, Dan Zhang, Shufang Liang","doi":"10.1111/1751-7915.70110","DOIUrl":"https://doi.org/10.1111/1751-7915.70110","url":null,"abstract":"Daptomycin (DAP) is a cyclic lipopeptide antibiotic produced by Streptomyces roseosporus, and its biosynthesis is precisely regulated by a complex regulatory network. Although the biosynthetic pathway of DAP has been elucidated, the regulatory mechanism governing its biosynthesis at the transcriptional level is not yet fully understood. In the present study, a new transcriptional regulator, DhyR, was identified. A deletion mutant of dhyR was constructed using the CRISPR-Cas9 tool to elucidate the biological role of DhyR thanks to functional and transcriptomic analyses. The results demonstrated that DhyR positively regulates DAP biosynthesis in S. roseosporus. The in-frame deletion of the dhyR gene resulted in a significant downregulation of the transcription levels of all structural genes within the DAP biosynthetic gene cluster and a significant decrease in DAP yield. In contrast, overexpression of dhyR enhanced the transcription levels of the DAP biosynthetic gene cluster, leading to a 23% increase in DAP yield. Deletion of dhyR caused significant changes in the expression of multiple genes involved in carbohydrate metabolism, energy metabolism and amino acid metabolic pathways through transcriptome analysis. Especially, deletion of dhyR led to a significant downregulation of transcription levels of three DAP biosynthesis-associated genes, including atrA, depR1 and ssig-05090. In summary, DhyR positively regulates DAP biosynthesis in S. roseosporus by influencing the expression of the DAP gene cluster and modulating precursor flux. It functions as a pleiotropic regulator of primary and secondary metabolism in S. roseosporus.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-03-02 DOI: 10.1111/1751-7915.70112

Pedro José Reyes-Pérez, Irene Jiménez-Guerrero, Ana Sánchez-Reina, Cristina Civantos, Natalia Moreno-de Castro, Francisco Javier Ollero, Jacinto Gandullo, Patricia Bernal, Francisco Pérez-Montaño

{"title":"The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin","authors":"Pedro José Reyes-Pérez, Irene Jiménez-Guerrero, Ana Sánchez-Reina, Cristina Civantos, Natalia Moreno-de Castro, Francisco Javier Ollero, Jacinto Gandullo, Patricia Bernal, Francisco Pérez-Montaño","doi":"10.1111/1751-7915.70112","DOIUrl":"https://doi.org/10.1111/1751-7915.70112","url":null,"abstract":"The symbiotic relationship between rhizobia and legumes is critical for sustainable agriculture and has important economic and environmental implications. In this intricate process, rhizobial bacteria colonise plant roots and induce the formation of specialised plant organs, the nodules. Within these structures, rhizobia fix environmental nitrogen into ammonia, significantly reducing the demand for synthetic fertilisers. Multiple bacterial secretion systems (TXSS, Type X Secretion System) are involved in establishing this symbiosis, with T3SS being the most studied. While the Type 6 Secretion System (T6SS) is known as a “nanoweapon” commonly used by diderm (formerly gram-negative) bacteria for inter-bacterial competition and potentially manipulating eukaryotic cells, its precise role in legume symbiosis remains unclear. Sinorhizobium fredii USDA257, a fast-growing rhizobial strain capable of nodulating diverse legume plants, possesses a single T6SS cluster containing genes encoding structural components and potential effectors that could target plant cells and/or act as effector-immunity pairs. Our research reveals that this T6SS can be induced in nutrient-limited conditions and, more importantly, is essential for successful nodulation and competitive colonisation of Glycine max cv Pekin. Although the system did not demonstrate effectiveness in eliminating competing bacteria in vitro, its active presence within root nodules suggests a sophisticated role in symbiotic interactions that extends beyond traditional interbacterial competition.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in 16S rRNA-Based Microbial Biomarkers for Gastric Cancer Diagnosis and Prognosis

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-02-24 DOI: 10.1111/1751-7915.70115

Yingying Wang, Xunan Qiu, Aining Chu, Jijun Chen, Lu Wang, Xiaohu Sun, Bengang Wang, Yuan Yuan, Yuehua Gong

{"title":"Advances in 16S rRNA-Based Microbial Biomarkers for Gastric Cancer Diagnosis and Prognosis","authors":"Yingying Wang, Xunan Qiu, Aining Chu, Jijun Chen, Lu Wang, Xiaohu Sun, Bengang Wang, Yuan Yuan, Yuehua Gong","doi":"10.1111/1751-7915.70115","DOIUrl":"https://doi.org/10.1111/1751-7915.70115","url":null,"abstract":"Gastric cancer (GC) is a malignant tumour with high morbidity and mortality worldwide, and there is an urgent need for early diagnosis and precision treatment. In recent years, the role of microbiota in the occurrence and development of GC has drawn extensive attention. Particularly, the in-depth study of GC-related microbiota by 16S rRNA sequencing technology has offered valuable tools and new perspectives for exploring the microbial characteristics of GC patients. This review systematically summarises the microbial diversity and composition of GC and non-GC samples based on 16S rRNA data, outlines the progress in identifying GC-related microbial biomarkers, explores the potential mechanisms by which diagnostic microbial biomarkers influence the development of GC, and reflects on the limitations of present studies. By integrating the current evidence, this review intends to offer a new theoretical foundation and further direction for the clinical translation of microbiota research in the diagnosis and treatment of GC.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoassay Detection of SARS-CoV-2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-02-24 DOI: 10.1111/1751-7915.70117

Robert M. Hnasko, Alice V. Lin, Jeffery A. McGarvey, Eric S. Jackson

{"title":"Immunoassay Detection of SARS-CoV-2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein","authors":"Robert M. Hnasko, Alice V. Lin, Jeffery A. McGarvey, Eric S. Jackson","doi":"10.1111/1751-7915.70117","DOIUrl":"https://doi.org/10.1111/1751-7915.70117","url":null,"abstract":"Immunoassays represent sensitive, easy-to-use, and cost-effective tests useful for the detection of the SARS-CoV-2 virus. In this manuscript, we report on the binding specificity of a pair of novel monoclonal antibodies (MAbs) generated against the SARS-CoV-2 nucleocapsid protein (NP) and their development into sensitive sandwich enzyme-linked immunosorbent assays (sELISA) and a lateral flow immunoassay (LFIA). Binding of these MAbs to hCoVs is limited to variants of SARS-CoV-2 and SARS-CoV NP. Chemiluminescent and absorbance spectroscopy sELISAs report a limit of detection (LOD) for the SARS-CoV-2 B.1.1.529 NP variant at 15 pg/mL, and the LFIA using a red-dyed 200 nm particle at 10 ng/mL. The sELISA exhibits broad SARS-CoV-2 viral variant detection with assay LOD for SARS-CoV-2 B.1.1.529 virus at 1.4 × 105 genome copies per mL (p ≤ 0.001). The availability of these MAbs should facilitate continued investment in the commercial development of immunoassays to increase global SARS-CoV-2 detection technologies.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alichondrichlorin, a Novel Chlorohydrin-Containing Natural Product With Tumoral Cytotoxic Activity Isolated From the Planctomycetota Bacterium Alienimonas chondri LzC2T

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-02-24 DOI: 10.1111/1751-7915.70076

Inês R. Vitorino, José D. N. Santos, Gloria Crespo, Ignacio Pérez-Victoria, Jesús Martín, Lorena Rodriguez, Maria C. Ramos, Teresa P. Martins, Pedro N. Leão, Francisca Vicente, Vítor Vasconcelos, Olga M. Lage, Fernando Reyes

{"title":"Alichondrichlorin, a Novel Chlorohydrin-Containing Natural Product With Tumoral Cytotoxic Activity Isolated From the Planctomycetota Bacterium Alienimonas chondri LzC2T","authors":"Inês R. Vitorino, José D. N. Santos, Gloria Crespo, Ignacio Pérez-Victoria, Jesús Martín, Lorena Rodriguez, Maria C. Ramos, Teresa P. Martins, Pedro N. Leão, Francisca Vicente, Vítor Vasconcelos, Olga M. Lage, Fernando Reyes","doi":"10.1111/1751-7915.70076","DOIUrl":"https://doi.org/10.1111/1751-7915.70076","url":null,"abstract":"To address the on-going need for chemical novelty and the limited information on Planctomycetota secondary metabolism, we focused on exploring the recently isolated marine planctomycetal strain Alienimonas chondri LzC2T to uncover its potential production of novel compounds. This work contemplates the description of a large-scale cultivation study of strain LzC2T, followed by metabolite extraction and compound isolation using chromatographic approaches, which resulted in the isolation of a novel molecule designated as alichondrichlorin. Structural elucidation of this new molecule was accomplished by a combination of high-resolution mass spectrometry and nuclear magnetic resonance. The molecule was additionally screened for anti-proliferative bioactivity against human tumoral and non-tumoral cell lines. These cytotoxicity assays revealed a targeted effect of alichondrichlorin in the growth of tumoral cell lines, especially human breast adenocarcinoma MCF-7 cell line (EC50 = 4.06 μM) without effect on the human non-tumoral THLE-2 cell line (EC50 > 50 μM).","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0