Molecular Strategies to Overcome Fungal Virulence in Crop Protection

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-14 DOI:10.1111/1751-7915.70220

C. A. Molina-Santiago, D. Vela-Corcía

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Abstract

Fungal pathogens are major threats to global crop production, intensified by rising fungicide resistance and the limited availability of resistant cultivars. This highlight article outlines recent molecular strategies aimed at reducing fungal virulence through sustainable and targeted approaches. RNA interference (RNAi) has emerged as a precise method to silence essential genes in pathogens, significantly impairing virulence and development. In parallel, inhibiting fungal efflux transporters—particularly ABC and MFS proteins—has been shown to reverse multidrug resistance and restore fungicide efficacy in pathogens like Botrytis cinerea. Additionally, engineering biocontrol agents expressing anti-apoptotic genes enhances their growth, stress resistance, and mycoparasitic activity. These strategies collectively illustrate the potential of combining RNAi technologies, efflux inhibition, and genetically enhanced biocontrol agents to create integrated, environmentally friendly plant protection systems. Such precision-targeted approaches represent a promising alternative to traditional chemical control, aligning with global efforts to achieve sustainable agriculture.

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作物保护中克服真菌毒力的分子策略

真菌病原体是全球作物生产的主要威胁，而杀菌剂耐药性的提高和抗真菌品种的有限供应加剧了这一威胁。这篇重点文章概述了最近的分子策略，旨在通过可持续和有针对性的方法减少真菌的毒力。RNA干扰（RNAi）已成为一种精确的方法来沉默病原体中的必需基因，显著损害毒力和发育。与此同时，抑制真菌外排转运蛋白——尤其是ABC和MFS蛋白——已被证明可以逆转多药耐药性，并恢复灰葡萄孢等病原体的杀菌剂功效。此外，表达抗凋亡基因的工程生物防治剂可提高其生长、抗逆性和真菌活性。这些策略共同说明了结合RNAi技术、外排抑制和基因增强生物防治剂来创建综合的、环境友好的植物保护系统的潜力。这种精确定向的方法代表了传统化学控制的一种有希望的替代方法，与实现可持续农业的全球努力保持一致。

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来源期刊

Microbial Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

11.20

自引率

3.50%

发文量

162

审稿时长

1 months

期刊介绍： Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes