Microbial Biotechnology最新文献

{"title":"In Vivo Enzyme Catalytic Rates in Formate-Growing Methanococcus maripaludis","authors":"Celma Mekki,&nbsp;Hamza Faquir,&nbsp;Enrique de Dios Mateos,&nbsp;Silvan Scheller,&nbsp;Paula Jouhten","doi":"10.1111/1751-7915.70417","DOIUrl":"10.1111/1751-7915.70417","url":null,"abstract":"<p>Turnover numbers (<span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>k</mi>\u0000 <mi>cat</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {k}_{mathrm{cat}} $$</annotation>\u0000 </semantics></math>) describe intrinsic catalytic capacities of enzymes. Although they have been characterized in vitro for many model organisms, such data is scarce for anaerobic Archaea like the methanogen <i>Methanococcus maripaludis</i>. Moreover, the apparent in vivo catalytic rates of enzymes operating in C1 utilization have neither been quantified experimentally nor predicted computationally. Here, we determined the in vivo catalytic rates of 99 <i>M. maripaludis</i> enzymes during growth on formate as the sole carbon source and electron donor. The determination was performed using previously published proteomics data and genome-scale metabolic model simulations constrained with experimental data across multiple growth rates. The obtained in vivo catalytic rates (<span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>k</mi>\u0000 <mi>app</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {k}_{mathrm{app}} $$</annotation>\u0000 </semantics></math>) were compared to sequence-derived maximum turnover numbers (<span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>k</mi>\u0000 <mi>cat</mi>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {k}_{mathrm{cat}} $$</annotation>\u0000 </semantics></math>) previously predicted by a machine-learning model trained on in vitro data. Sequence-derived turnover numbers and maximum in vivo catalytic rates did not correlate. The quantitative insight into methanogenic C1 metabolism and the in vivo catalytic rates can be used to guide metabolic engineering strategies. Engineered methanogens can serve as hosts in disruptive solutions of future biotechnological chemical production from one-carbon compounds.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13400843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symbiont-Mediated Detoxification of Xenobiotics in Honey Bees","authors":"Yao Liu,&nbsp;Esther du Rand,&nbsp;Christian Pirk,&nbsp;Huoqing Zheng","doi":"10.1111/1751-7915.70415","DOIUrl":"10.1111/1751-7915.70415","url":null,"abstract":"<p>The active foraging behaviour of honey bees frequently exposes them to various xenobiotics. Honey bees rely primarily on endogenous enzymatic detoxification systems to metabolise these compounds; however, this capacity is constrained by limitations in their genomic detoxification repertoire. The gut microbiota may partially compensate for this deficiency through two complementary mechanisms: directly transforming or sequestering xenobiotics, and modulating host detoxification pathways. We therefore propose that the gut microbiota should be regarded as an extended detoxification organ in honey bees. This perspective also points to a microbial biotechnology agenda for pollinator protection, including precision probiotics, microbiome-informed breeding and engineered symbionts. Viewing detoxification as a holobiont trait provides a more comprehensive framework for understanding bee resilience and for developing microbiome-based interventions under real-world chemical stress.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13389231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148547190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-Glycosylation Influences the Heterologous Expression of an Unspecific Peroxygenase From Marasmius rotula in Saccharomyces cerevisiae","authors":"Marina Schramm,&nbsp;Kai-Uwe Schmidtke,&nbsp;Yvonne Kolaczek,&nbsp;Nico Jehmlich,&nbsp;Martin Hofrichter,&nbsp;Katrin Scheibner","doi":"10.1111/1751-7915.70418","DOIUrl":"10.1111/1751-7915.70418","url":null,"abstract":"<p><i>N</i>-Linked glycosylation can have a significant impact on the yield of heterologously expressed proteins secreted by <i>Saccharomyces cerevisiae</i>. The yeast is a widely used host for the expression of unspecific peroxygenases (UPOs), a subclass of peroxide-dependent oxidoreductases with high potential for industrial biocatalytic applications. However, the effects of <i>N</i>-glycosylation on the expression of recombinant UPOs have not yet been investigated. Here, we studied respective protein modification on the expression of a UPO from <i>Marasmius rotula</i>, belonging to the protein subfamily of short peroxygenases, in <i>S. cerevisiae</i>. Two of the three <i>N</i>-glycosylation sites that are actually occupied in r<i>Mro</i>UPO were eliminated by substituting asparagine at positions N43 and N151 with serine. The single substitutions led to reduced amounts of secreted r<i>Mro</i>UPO, with N43S having the highest impact (almost four times lower protein amount compared to the native enzyme) and N151S having a moderate effect. In the next step, the glycosylation probability at position 43 was enhanced by replacing the serine in the corresponding sequon with threonine, which increased the expression of the r<i>Mro</i>UPO variant. The concentration of active UPO in the concentrated culture supernatant of the glycosylation-optimised variant S45T was twice as high as that of the native r<i>Mro</i>UPO. The results suggest that <i>N</i>-linked glycosylation is an important factor for successful heterologous UPO expression in <i>S. cerevisiae</i>.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13382365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retirement Changed My Laboratory, Not My Mission","authors":"José A. Gil","doi":"10.1111/1751-7915.70419","DOIUrl":"10.1111/1751-7915.70419","url":null,"abstract":"<p>Retirement need not mark the end of a scientific career. This opinion article describes how the MicroMundo citizen science programme transformed a secondary-school laboratory into a place where students search for new antibiotics while experiencing authentic scientific research. The experience highlights the educational value of citizen science, promotes microbial literacy and STEM vocations, and illustrates how retired scientists can continue to contribute meaningfully by connecting universities, schools and society.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation and Scale-Up of the Heterologous Production of the Antifungal Protein PeAfpA From Penicillium expansum in Stirred-Tank Bioreactors","authors":"Stefani de Ovalle,&nbsp;Sandra Garrigues,&nbsp;Jose V. Gimeno-Alcañiz,&nbsp;Josep V. Mercader,&nbsp;Antonio Abad-Fuentes,&nbsp;Jose F. Marcos,&nbsp;Paloma Manzanares","doi":"10.1111/1751-7915.70414","DOIUrl":"10.1111/1751-7915.70414","url":null,"abstract":"<p>Antifungal proteins from fungal origin (AFPs) are small, secreted, cationic, cysteine-rich proteins with potential as novel biofungicides. However, their application requires safe and cost-effective production platforms. PeAfpA is a highly active AFP naturally produced in large quantities by the phytopathogenic fungus <i>Penicillium expansum</i> and biotechnologically produced by the Generally Recognized As Safe fungus <i>Penicillium chrysogenum</i> although at lower yields<i>.</i> Here, the production of PeAfpA using the <i>P. chrysogenum</i> strain PCMG11552 was optimised in 0.5 L and scaled up to 5 L in stirred-tank bioreactors. A competitive enzyme-linked immunosorbent assay (cELISA) was developed to evaluate the influence of process parameters on PeAfpA production, identifying medium pH and agitation rate as key factors, while dissolved oxygen proved critical during scale-up. The PeAfpA yields obtained were tenfold higher than those achieved previously in flask cultures, demonstrating the feasibility of scaling AFP production from laboratory to pilot scale for diverse applications. Finally, the <i>P. chrysogenum</i> marker-free PeAfpA-producing strain obtained via recyclable CRISPR/Cas9 system guarantees regulatory compliance and biosafety, making it suitable for its use in any commercial environment.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70414","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148443566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Experimentally Verified Mechanistic Model for Predicting Quorum Sensing-Based Switches","authors":"Jasmine De Baets,&nbsp;Marjan De Mey,&nbsp;Brecht De Paepe","doi":"10.1111/1751-7915.70408","DOIUrl":"10.1111/1751-7915.70408","url":null,"abstract":"<p>Quorum sensing-based genetic circuits are gaining traction in synthetic biology as they link population-level behaviour to individual cell responses. However, tuning these circuits remains challenging due to complex dynamics, particularly during the ‘Learn’ phase of the Design-Build-Test-Learn (DBTL) cycle. To accelerate this process, we developed a mathematical model to predict how varying expression levels of the transcription factor and synthase affect the response of the EsaI/EsaR quorum sensing system. The EsaI/EsaR system consists of the EsaI synthase, which produces the autoinducer 3-oxo-hexanoyl homoserine lactone, and the EsaR transcription factor, which regulates gene expression in response to the autoinducer concentration. A strain library was constructed, and experimental data were used to optimize the model. The final model could successfully differentiate between the effects of these expression levels on the response of the bidirectional promoter. It allowed visualization of all potential system outcomes and emphasized the transcription factor's critical role in tuning the circuit. This model offers a valuable tool for fine-tuning EsaI/EsaR-based systems for synthetic biology applications. Moreover, given the homology within the LuxR-family quorum sensing systems, this modelling approach may serve as a foundation for model-based tuning of other quorum sensing systems.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70408","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148434614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytodetoxification of Lindane (γ-Hexachlorocyclohexane) With Plants Expressing a Bacterial Dechlorinase","authors":"Carolina Gonzalez De Figueras,&nbsp;Pablo Catarecha,&nbsp;César Menor-Salván,&nbsp;Jorge Díaz-Rullo,&nbsp;Antonio Leyva,&nbsp;Eduardo González Pastor,&nbsp;Víctor de Lorenzo","doi":"10.1111/1751-7915.70409","DOIUrl":"10.1111/1751-7915.70409","url":null,"abstract":"<p>The construction and performance of plants capable of detoxifying the organochlorine pesticide (lindane, γ-hexachlorocyclohexane or γ-HCH) is reported. To this end, the bacterial <i>linA</i> gene from <i>Sphingobium japonicum</i> strain UT26, which encodes a dehydrochlorinase initiating γ-HCH degradation, was engineered into <i>Arabidopsis thaliana</i>. The resulting lines expressing <i>linA</i> exhibited markedly enhanced tolerance to lindane compared to wild-type controls, both in synthetic media and in contaminated soils. Furthermore, thereby engineered plants also removed more than 90% of the pollutant from the medium within 4 weeks. Chemical analyses revealed not only the formation of 1,2,4-trichlorobenzene (TCB), the expected γ-HCH degradation intermediates, but also detected 1,4-dichlorobenzene (DCB), suggesting that native plant activities could further push the degradation process beyond the canonical microbial pathway. This points to a potentially synergistic interaction between the introduced bacterial enzyme and the endogenous plant detoxification systems. By combining bacterial catabolic activity with plant resilience and root-mediated soil interactions, our data advocate a new strategy for the remediation of γ-HCH contaminated environments and demonstrate the feasibility of designing plants for enhanced degradation of persistent organic pollutants.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148434691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Genomic Analysis of the First Salmonella typhimurium Monophasic Variant 1,4,[5],12:i:- Isolate Harbouring a Chromosomally Integrated blaNDM-5","authors":"Shihan Zeng,&nbsp;Weilong Guo,&nbsp;Chengfeng Lin,&nbsp;Lianghui Peng,&nbsp;Jingjie Song","doi":"10.1111/1751-7915.70413","DOIUrl":"10.1111/1751-7915.70413","url":null,"abstract":"<p>We report the first clinical strain of the monophasic variant of <i>Salmonella typhimurium</i> (serotype 1,4,[5],12:i:-) belonging to ST34 with chromosomally integrated <i>bla</i>_NDM-5. Although chromosomal integration of <i>bla</i>_NDM-5 has been described in other <i>Enterobacterales</i> and <i>Salmonella</i> serovars, this is the first such report in this high-risk clone. This study characterises the genomic and epidemiological background of this strain. The isolate (1111-90), obtained from a paediatric patient, was subjected to antimicrobial susceptibility testing, whole-genome sequencing and comparative genomic analysis against 674 complete <i>bla</i>_NDM-5-positive plasmids retrieved from the PlasmidScope database. The isolate exhibited a multidrug-resistant phenotype, including carbapenem resistance, while retaining susceptibility to tigecycline and aztreonam. It harboured 13 resistance genes distributed across the chromosome and two plasmids. The ~11 kb chromosomally integrated <i>bla</i>_NDM-5 segment is flanked by directly oriented IS<i>26</i> elements, forming an IS<i>26</i>-bounded composite transposon with no canonical target-site duplication at its boundaries. Phylogenetic analysis suggested that this chromosomal segment likely originated from IncX3 or IncI1 plasmid lineages prevalent in China. Comparative genetic analysis highlighted the significant role of mobile genetic elements (IS<i>26</i>, IS<i>5</i> and IS<i>3000</i>) in the spread of <i>bla</i>_NDM-5 within <i>S. typhimurium</i>. The chromosomal integration of an IS<i>26</i>-bounded <i>bla</i>_NDM-5 module in <i>S</i>. 1,4,[5],12:i:- ST34 suggests the emergence of a stable, high-risk carbapenem-resistant clone, underscoring the need for genomic surveillance to track IS<i>26</i>-mediated chromosomal integration events.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Silencing of Aminoglycoside Biosynthesis for the Discovery of Novel Antimicrobials in Streptomyces","authors":"Yuncong Xu,&nbsp;Yali Du,&nbsp;Jiarong Yu,&nbsp;Yinghui Zhao,&nbsp;Zhenlin Li,&nbsp;Jufen Wang,&nbsp;Yijun Chen,&nbsp;Deli Xiao,&nbsp;Xuri Wu","doi":"10.1111/1751-7915.70407","DOIUrl":"10.1111/1751-7915.70407","url":null,"abstract":"<p><i>Streptomyces</i> species are prolific sources of antimicrobials, yet their vast biosynthetic capacity remains underexploited due to the rediscovery of common antibiotics (e.g., ~1% for streptomycin and ~10% for streptothricin). To address this challenge, we developed a dual-layer biosynthetic blockade system, designated CI-ARS³, which integrates two orthogonal silencing modules: a competitive transcriptional silencer that represses transcription of the native biosynthetic gene cluster (BGC), and an antisense RNA-based silencer that facilitates the degradation of BGC-derived mRNAs and/or translational inhibition. Implemented on a single <i>attB</i> site–integrating plasmid (pSET152-<i>aafssf</i>) encoding both modules, CI-ARS³ robustly suppresses streptomycin and streptothricin biosynthesis across 54 <i>Streptomyces</i> strains, achieving an inhibition efficiency of over 97% with high specificity for the target compounds under the tested conditions. The removal of these dominant backgrounds led to the discovery of enissomycin, a previously unreported antibacterial molecule. Enissomycin exhibited potent activity against <i>Acinetobacter baumannii</i> (MIC 4–8 μg/mL) and exhibited no significant cytotoxicity, highlighting its promise for drug development. Collectively, CI-ARS³ is a robust platform for the efficient dereplication of streptomycin and streptothricin, thereby expediting the discovery of novel antimicrobial compounds from <i>Streptomyces</i>.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70407","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Mycosynthesis to Immunomodulation: Exploring the Safety, Adjuvant Potential and Microbiota Impact of Biogenic Silver Nanoparticles","authors":"Ana Clara Muniz-Lagos,&nbsp;Diego M. Roldán,&nbsp;Paula Arbildi,&nbsp;Gustavo Mourglia-Ettlin,&nbsp;Silvana Alborés","doi":"10.1111/1751-7915.70411","DOIUrl":"10.1111/1751-7915.70411","url":null,"abstract":"<p>Subunit vaccines are considered the safest immunization platforms but typically require adjuvants to elicit robust immune responses. Although nanoparticles have emerged as promising adjuvants, biogenic silver nanoparticles (AgNPs) produced by mycosynthesis remain largely unexplored. Herein, nine different AgNPs were mycosynthesized using local strains of <i>Phanerochaete chrysosporium</i>, <i>Penicillium expansum</i> and <i>Punctularia atropurpurascens</i> grown in three different culture media (PDB, MEB, MGYP). Mycosynthesized AgNPs were all shown to be spherical, negatively charged and containing fungal proteins within the biomolecular capping. Additionally, although in vitro biocompatibility assessments showed AgNPs-specific differences in haemolytic and cytotoxic activities, no signs of acute toxicity were observed in mice. The adjuvant potential of AgNPs was further evaluated in ovalbumin-immunized mice, showing that four AgNPs displayed significant immunoenhancement activity with an outstanding IgG_2a-polarizaing effect. Adjuvant and IgG subclass polarizing activities were both shown to be dose dependent. Finally, distal effects induced by selected AgNPs were assessed through faecal microbiota analyses. Remarkably, while <i>Firmicutes</i> and <i>Bacteroidota</i> remained the dominant phyla, AgNPs-treated groups showed compositional shifts consistent with mild immunomodulation. Summing up, our results on biocompatibility, adjuvant capacity, strong and dose-dependent IgG_2a-polarizing activity, as well as limited impact on gut microbiota, support mycosynthesized AgNPs as versatile next-generation platforms for developing novel vaccine adjuvants.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 7","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148417160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}