Microbial Biotechnology最新文献_第5页

Establishment of CRISPR-STAR System to Realise Simultaneous Transcriptional Activation and Repression in Yarrowia lipolytica 建立CRISPR-STAR系统实现脂性耶氏菌转录同时激活和抑制

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-24 DOI: 10.1111/1751-7915.70151

Yaru Chen, Mengxu Li, Xuanwei Liu, Qiyang Duan, Lin Xiao, Luxin Wang, Congcong Huang, Hao Song, Yingxiu Cao

{"title":"Establishment of CRISPR-STAR System to Realise Simultaneous Transcriptional Activation and Repression in Yarrowia lipolytica","authors":"Yaru Chen, Mengxu Li, Xuanwei Liu, Qiyang Duan, Lin Xiao, Luxin Wang, Congcong Huang, Hao Song, Yingxiu Cao","doi":"10.1111/1751-7915.70151","DOIUrl":"https://doi.org/10.1111/1751-7915.70151","url":null,"abstract":"The ability to regulate gene expression in multiple directions is crucial to maximise the production of microbial cell factories. However, the lack of a regulatory tool that can simultaneously activate and repress multiple genes restricts the manipulation diversity of Yarrowia lipolytica, which is an industrial workhorse for bioproduction. To address this issue, we developed a CRISPR scaffold RNAs (scRNAs)-mediated transcriptional activation and repression (CRISPR-STAR) platform. Firstly, we evaluated different methods for bidirectional regulation using CRISPR on both endogenous and synthetic promoters in Y. lipolytica, and chose the utilisation of orthogonal scRNAs to recruit activation and inhibition domains. Secondly, CRISPR-STAR was optimised by the introduction of alternative dCas proteins, scRNA structures and activators. 2.6-fold and 54.9-fold activation were achieved for synthetic and endogenous promoters, respectively, when the VPR transcriptional activator was recruited via MS2 hairpin. The repression of several genes was successfully achieved, with repression levels ranging from 3% to 32%, when the MXI1 transcriptional repressor was recruited via PP7 hairpin. Finally, CRISPR-STAR was applied to enhance fatty alcohol production by activating the FAR gene (encodes fatty acyl-CoA reductase) and repression of the PEX10 gene (encodes an integral membrane protein required for peroxisome biogenesis and matrix protein import). Compared to the non-targeting control, the bidirectionally regulated strain showed a 55.7% increase in yield to 778.8 mg/L. Our findings demonstrate that the CRISPR-STAR platform enables multi-mode regulation of genes, offering engineering opportunities to improve the productive performance of Y. lipolytica.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Glucose to Green Chemistry: Breakthrough in Microbial Production of Tartaric Semialdehyde 从葡萄糖到绿色化学：酒石酸半醛微生物生产的突破

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-23 DOI: 10.1111/1751-7915.70149

Shuangxi Li, Lingcheng Li, Qiwu Jiang, Jianfeng Wang, Xiaoming Sun, Liangliang Zhang, Jianfeng Yuan

{"title":"From Glucose to Green Chemistry: Breakthrough in Microbial Production of Tartaric Semialdehyde","authors":"Shuangxi Li, Lingcheng Li, Qiwu Jiang, Jianfeng Wang, Xiaoming Sun, Liangliang Zhang, Jianfeng Yuan","doi":"10.1111/1751-7915.70149","DOIUrl":"https://doi.org/10.1111/1751-7915.70149","url":null,"abstract":"L-(+)-tartaric acid (L-TA) is a crucial hydroxy carboxylic chelator with extensive applications in the food and pharmaceutical industries. The synthesis of L-TA from renewable biomass presents a promising approach to mitigating environmental impact and advancing green energy initiatives. Previous studies revealed that a mutant transketolase (TKTA_M) could catalyse the production of tartaric semialdehyde, a precursor to L-TA. This study focuses on the development of a Gluconobacter oxydans cell factory for tartaric semialdehyde production, employing a combination of metabolic engineering and a modular strategy. The genetically modified G. oxydans T strain exhibited robust expression of the tktA_M gene. The optimal pH and temperature for this strain were determined to be 6.0°C and 30°C, respectively. Under these conditions, the strain produced 32.21 ± 0.74 g/L of tartaric semialdehyde from glucose. Implementation of a “Push-Pull” strategy enhanced tartaric semialdehyde production, resulting in a 23.85% increase in the G. oxydans T02 cell growth. In CSLP medium with 100 g/L glucose, the fermentation process yielded 48.88 ± 2.16 g/L of tartaric semialdehyde and 7.72 ± 1.56 g/L of residual 5-KGA after 48 h. This resulted in a tartaric semialdehyde productivity rate of 1.018 g/L·h, representing an 87.82% improvement over flask fermentation. This study demonstrates a straightforward and efficient microbial process for the oxidation of glucose to tartaric semialdehyde, indicating its potential for industrial-scale production and facilitating the synthesis of L-TA from renewable resources.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Sugar Metabolism During Fermentation of Brewers' Spent Grain by Leuconostoc pseudomesenteroides DSM20193 假肠白菌DSM20193对啤酒废粮发酵过程中糖代谢的调控

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-21 DOI: 10.1111/1751-7915.70116

Koirala Prabin, Maina Ndegwa, Mojzita Dominik, Coda Rossana

{"title":"Regulation of Sugar Metabolism During Fermentation of Brewers' Spent Grain by Leuconostoc pseudomesenteroides DSM20193","authors":"Koirala Prabin, Maina Ndegwa, Mojzita Dominik, Coda Rossana","doi":"10.1111/1751-7915.70116","DOIUrl":"https://doi.org/10.1111/1751-7915.70116","url":null,"abstract":"Re-utilising brewers' spent grain (BSG) through LAB fermentation can enable its broad use in the food industry, enhancing its nutritional and functional properties and offering a clear example of a sustainable approach in the valorisation of food side streams. Despite extensive research on LAB fermentation, the regulation of metabolism during the growth in complex food-industry-relevant environments remains unclear. This study investigates the metabolic processes in Leuconostoc pseudomesenteroides DSM20193 during 24 h fermentation of BSG with and without 4% sucrose (w/w) supplementation, allowing in situ dextran synthesis. Besides dextran synthesis, the presence of sucrose led to faster acidification, especially due to the increased formation of acetic acid. Furthermore, differences in the utilisation of sucrose, fructose, glucose, and maltose and the formation of diverse oligosaccharides were observed. Transcriptome analysis comparing expression profiles during 0 h and 16 h growth in BSG with sucrose revealed differences in the expression of genes involved in carbohydrate utilisation pathways, including higher activity of sucrose and maltose metabolism and lower activity of metabolism related to alternative carbon sources. Transcription analysis of selected relevant genes in a time-course comparison between BSG with and without sucrose provided more detailed indications of responses of the metabolic network in this complex environment. This analysis provided a deeper understanding of the dynamic regulatory mechanism that drives sugar metabolism and dextran synthesis and how the presence of sucrose can alter the metabolic flux towards different fermentation products.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined Isotopic Tracer and Modelling Approach Reveals Differences in Nitrogen Metabolism in S. cerevisiae, S. uvarum and S. kudriavzevii Species 联合同位素示踪和建模方法揭示了S. cerevisiae， S. uvarum和S. kudriavzevii种氮代谢的差异

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-18 DOI: 10.1111/1751-7915.70087

Romain Minebois, David Henriques, Eva Balsa-Canto, Amparo Querol, Carole Camarasa

{"title":"Combined Isotopic Tracer and Modelling Approach Reveals Differences in Nitrogen Metabolism in S. cerevisiae, S. uvarum and S. kudriavzevii Species","authors":"Romain Minebois, David Henriques, Eva Balsa-Canto, Amparo Querol, Carole Camarasa","doi":"10.1111/1751-7915.70087","DOIUrl":"https://doi.org/10.1111/1751-7915.70087","url":null,"abstract":"The species Saccharomyces uvarum and Saccharomyces kudriavzevii have gained popularity in recent decades due to their interesting oenological properties. However, although it plays a crucial role in yeast fermentation performance and compound synthesis, our understanding of nitrogen metabolism in these species remains limited. Therefore, we compared how three strains of Saccharomyces cerevisiae, Saccharomyces uvarum and Saccharomyces kudriavzevii use relevant nitrogen sources by combining quantitative analysis approaches based on isotopic tracing and modelling. The model we have developed aims to facilitate the calculation and interpretation of stable isotope data for other experiments, by providing easy visualisation of the results and predicting the kinetics of isotope incorporation beyond the sampling points. The three species exhibit significant variations in their nitrogen assimilation profile. They differ in the timing of uptake of ammonium, arginine and glutamine: Saccharomyces cerevisiae prefers glutamine, Saccharomyces kudriavzevii ammonium and Saccharomyces uvarum arginine. This contributes to a different pattern of nitrogen redistribution towards proteinogenic amino acids between strains at the start of the exponential phase, which fades on entering the stationary phase. Additionally, we found that the contribution of leucine and valine to isoamyl alcohol production varies between species; also, Saccharomyces kudriavzevii activates the synthesis of volatile compounds earlier.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-Step RAA and CRISPR-Cas13a Method for Detecting Influenza B Virus 一步RAA和CRISPR-Cas13a检测乙型流感病毒的方法

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-15 DOI: 10.1111/1751-7915.70144

Xinling Zhang, Shiyu Chen, Juezhuo Li, Dong-ang Liu, Jianxiu Lai, Xiangquan Song, Ruiyao Hu, Yuting Qiu, Keyi Chen, Yue Xu, Xiaoping Li

{"title":"One-Step RAA and CRISPR-Cas13a Method for Detecting Influenza B Virus","authors":"Xinling Zhang, Shiyu Chen, Juezhuo Li, Dong-ang Liu, Jianxiu Lai, Xiangquan Song, Ruiyao Hu, Yuting Qiu, Keyi Chen, Yue Xu, Xiaoping Li","doi":"10.1111/1751-7915.70144","DOIUrl":"https://doi.org/10.1111/1751-7915.70144","url":null,"abstract":"We developed a sensitive and specific method based on recombinase-aided amplification (RAA) and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 13a (Cas13a). This method, named CRISPR-based Rapid and Efficient Test (CRISPRET), is designed for the early diagnosis of Influenza B (FluB) with the aim of shortening its transmission chain. We identified conserved regions in the Influenza B Virus (IBV) NS gene and designed forward and reverse primers along with crRNAs. We then established and optimised the reaction system, and Nucleic Acid Positive Reference Materials of IBV were used to evaluate the detection limit (DL) of CRISPRET. Additionally, we collected 257 clinical samples, comprising 127 samples from patients with IBV infection and 130 samples from healthy individuals, and subjected them to dual detection using CRISPRET and qPCR to evaluate the positive predictive value (PPV), negative predictive value (NPV), sensitivity and specificity of CRISPRET. We designed one forward primer, two reverse primers, and two crRNAs to establish and optimise the CRISPR ET. The method demonstrated the DL of 500 copies·μL−1 when assisted by appropriate equipment. Despite requiring auxiliary equipment and a 30-min reaction, the CRISPR ET method enables the detection of IBV nucleic acid within approximately the first 5 min, achieving high sensitivity (100%), specificity (97.69%), PPV (97.69%) and NPV (100%), with a concordance rate of 98.83% to qPCR. CRISPRET offers a simple, field-applicable, one-step method for the rapid detection of IBV. It has strong potential for field-testing applications and intelligent integration into existing diagnostic systems.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the Microbial World: A Global Initiative for Education and Sustainability 解锁微生物世界：教育和可持续发展的全球倡议

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-07 DOI: 10.1111/1751-7915.70124

Juan L. Ramos, Rup Lal, Francisca Colom, Max Chavarria, Wei Huang, Yun Wang, Zulema Udaondo, Kenneth N. Timmis

引用次数: 0

Monkeypox Virus: WHO's Second Public Health Emergency of International Concern Within 2 Years 猴痘病毒：世卫组织两年内第二次引起国际关注的突发公共卫生事件

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-07 DOI: 10.1111/1751-7915.70142

Harald Brüssow

{"title":"Monkeypox Virus: WHO's Second Public Health Emergency of International Concern Within 2 Years","authors":"Harald Brüssow","doi":"10.1111/1751-7915.70142","DOIUrl":"https://doi.org/10.1111/1751-7915.70142","url":null,"abstract":"An upsurge of monkeypox disease (mpox) cases with clade I virus in Central Africa led WHO to declare a Public Health Emergency of International Concern for a second time shortly after the worldwide clade II mpox epidemic in 2022/3 among homosexual men. In the Democratic Republic of Congo (DRC), the annual incidence of clade I mpox, transmitted mostly from animal sources to children, increased 20-fold between 1980 and 2007; 60,000 mpox cases occurred between 2010 and 2023. The incidence again doubled between 2023 and 2024, showing a case fatality rate of 3.3%. A new clade Ib virus was detected in 2024 in eastern DRC where mostly adults were infected by heterosexual contact. Ib was recently introduced and showed a mutation spectrum of human-to-human transmission. Asymptomatic mpox infections, the release of infectious virus before symptom onset in a subgroup of cases, and superspreaders complicate containment measures during the 2022 epidemic. Isolation of cases until two consecutive negative PCR tests was recommended but necessitates cheap and rapid diagnostic tests which are in development. Sexual behavioural changes during the 2022 epidemic have contributed more to the curbing of the epidemic than vaccination. The smallpox vaccine Dryvax protected children exposed to clade I mpox in DRC in the 1980s. The attenuated third-generation smallpox Modified Vaccinia Ankara (MVA) vaccines and derivatives showed robust protection against clade IIb mpox during the 2022/3 epidemic in various study formats. Vaccine efficacy exceeding 75% was reported after two doses. mRNA in lipid-nanoparticle encoding surface proteins from extracellular enveloped and intracellular mature virions of monkeypox virus (MPXV) induced humoral and cellular immune responses that protected macaques against mpox disease with clade I and II viruses better than MVA. Only mixtures of monoclonal antibodies protected mice from mpox. The antiviral tecovirimat showed no efficacy in two clinical trials against clade I and II mpox.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineering of a Serotonin Overproducing Saccharomyces cerevisiae Strain 一株过量生产5 -羟色胺的酿酒酵母的代谢工程

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-05 DOI: 10.1111/1751-7915.70140

Andrés Planells-Cárcel, Elena Valera-García, Guillermo Quintas, José Luis Martínez, Sara Muñiz-Calvo, José Manuel Guillamón

{"title":"Metabolic Engineering of a Serotonin Overproducing Saccharomyces cerevisiae Strain","authors":"Andrés Planells-Cárcel, Elena Valera-García, Guillermo Quintas, José Luis Martínez, Sara Muñiz-Calvo, José Manuel Guillamón","doi":"10.1111/1751-7915.70140","DOIUrl":"https://doi.org/10.1111/1751-7915.70140","url":null,"abstract":"The EU Green Deal prioritises the transformation of the chemical industry to a more environmentally sustainable model. This involves using microorganisms, such as Saccharomyces cerevisiae, to produce molecules more sustainably through biotechnological approaches. In this study, we demonstrate an example of serotonin production using S. cerevisiae as a cell factory, along with its optimisation and upscaling. To achieve this, we introduced two heterologous genes, the combination of tryptophan decarboxylase from Clostridium sporogenes (CsTDC) and tryptamine 5-hydroxylase from Oryza sativa (OsT5H), to complete the serotonin biosynthetic pathway using L-tryptophan (L-TRP) as a precursor. By modifying ARO4 to a feedback-resistant version (ARO4*), the flux of the shikimate pathway was significantly increased and serotonin production was achieved at levels up to 120 mg/L directly from the glucose source. After a medium optimisation, a final concentration of 80 g/L glucose and 300 mg/L of nitrogen resulted in better conditions for increasing serotonin titres. Using this medium in a 1 L bioreactor fermentation resulted in approximately 250 mg/L of serotonin. A targeted metabolomic study of the bioreactor growth medium identified potential bottlenecks in the serotonin-overproducing strain and future targets for increasing its titre. We have constructed a strain of S. cerevisiae that represents the first steps towards feasible industrial production of serotonin using a sustainable and environmentally friendly approach, paving the way for the development of similar biotechnological strategies in the future.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gamifying Science—Novel Strategies for Bringing Microbiology to the Classroom 科学游戏化--将微生物学带入课堂的新策略

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-05 DOI: 10.1111/1751-7915.70141

Carlos del Cerro-Sánchez, Vanesa Amarelle, Raúl Platero, Danilo Pérez-Pantoja, Daniela Wicki-Emmenegger, Max Chavarría, Gonzalo Durante-Rodríguez

引用次数: 0

Exploring Yeast's Energy Dynamics: The General Stress Response Lowers Maintenance Energy Requirement 探索酵母的能量动态：一般应激反应降低维持能量需求

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-04-03 DOI: 10.1111/1751-7915.70126

Nuran Temelli, Simon van den Akker, Ruud A. Weusthuis, Markus M. M. Bisschops

{"title":"Exploring Yeast's Energy Dynamics: The General Stress Response Lowers Maintenance Energy Requirement","authors":"Nuran Temelli, Simon van den Akker, Ruud A. Weusthuis, Markus M. M. Bisschops","doi":"10.1111/1751-7915.70126","DOIUrl":"https://doi.org/10.1111/1751-7915.70126","url":null,"abstract":"In many microbial biotechnology processes, biomass itself is not the product of interest, but rather targeted chemicals or proteins. In these processes, growth should be limited to direct more substrate to product and increase process yields. Under growth-limiting conditions, such as nutrient limitation, microorganisms, including the yeast Saccharomyces cerevisiae, activate a general stress response (GSR). Different hypotheses have been formulated for this activation, including a preparatory role for future stresses or a role in cellular protein density. Here we tested a third hypothesis: the GSR reduces the energy needed to maintain cellular homeostasis, also known as the maintenance energy requirement (MER). The impact of GSR on MER was investigated by assessing the effect of the absence of its key regulators, Msn2 and Msn4, on energy-substrate distribution and stress resistance. Chemostat and fed-batch cultures revealed significant increases in MER of up to 85% in the deletion strain compared to the parental strain. In contrast, maximal biomass yields, growth rates and morphology were unaffected. Our insights highlight an additional role of the GSR, namely saving cellular energy. As the MER is a key determinant of product yields and in process design, especially in low growth processes, our findings can help to optimise microbial bioprocesses.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0