Microbial Biotechnology最新文献_第5页

Clinical Validation of Loop-Mediated Isothermal Amplification Assays for the Rapid Detection of Neisseria gonorrhoeae and Chlamydia trachomatis 环介导等温扩增法快速检测淋病奈瑟菌和沙眼衣原体的临床验证

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-29 DOI: 10.1111/1751-7915.70214

Yasaman Ahmadi, Yejiong Yu, Zhanfeng Cui, Wei E. Huang, Monique I. Andersson

{"title":"Clinical Validation of Loop-Mediated Isothermal Amplification Assays for the Rapid Detection of Neisseria gonorrhoeae and Chlamydia trachomatis","authors":"Yasaman Ahmadi, Yejiong Yu, Zhanfeng Cui, Wei E. Huang, Monique I. Andersson","doi":"10.1111/1751-7915.70214","DOIUrl":"https://doi.org/10.1111/1751-7915.70214","url":null,"abstract":"Neisseria gonorrhoeae (GC) and Chlamydia trachomatis (CT) are the predominant causes of bacterial sexually transmitted diseases. While nucleic acid amplification testing (NAATs), primarily polymerase chain reaction (PCR), is regarded as the gold standard for identifying these two pathogens, it usually takes a prolonged turnaround time and requires sophisticated equipment with considerable expense. In this study, we developed novel loop-mediated isothermal amplification (LAMP) assays for rapid detection (< 30 min) of GC and CT in clinical urine and swab specimens. We analysed 208 clinical samples with three different pre-treatment techniques including heating inactivation, centrifugation, and DNA extraction. LAMP results were compared with clinical results from the FDA-approved BD ProbeTec ET assay. After heating inactivation, LAMP detected merely 41% and 65% of BD-identified GC- and CT-positive samples, respectively. Introducing centrifugation as an affordable and rapid pre-treatment step increased detection rates to 81% and 91% for GC and CT, respectively. DNA extraction further enhanced the detection rates to 96% and 95% for GC- and CT-LAMP, respectively. All these LAMP assays exhibited clinical specificity of ≥ 98%, underscoring the specificity of the chosen target genes (the porA pesudogene for GC and the ftsK gene for CT). Discrepant samples were verified by real-time PCR; results were consistent with our LAMP findings. The overall LAMP performance met the WHO criteria for sensitivity and specificity for GC/CT point-of-care testing.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70214","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the Potential of mRNA Vaccines Against Infectious Diseases 利用mRNA疫苗对抗传染病的潜力

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-25 DOI: 10.1111/1751-7915.70212

Nouran Rezk, Siobhán McClean

{"title":"Harnessing the Potential of mRNA Vaccines Against Infectious Diseases","authors":"Nouran Rezk, Siobhán McClean","doi":"10.1111/1751-7915.70212","DOIUrl":"10.1111/1751-7915.70212","url":null,"abstract":"mRNA vaccines have emerged as promising alternatives to conventional vaccines because of their flexible design, high immunogenicity, favourable safety profile, efficacy and potential for rapid clinical development. The accelerated development of mRNA vaccines during the COVID-19 pandemic has revolutionised the field of vaccinology, highlighting their potential for combating emerging infectious diseases. The mRNA platforms can induce robust humoral as well as CD4+ and CD8+ T-cell-mediated immunity, offering broader protection than subunit protein vaccines. Consequently, they have been extensively studied against a wide range of viral, bacterial and parasitic infections, although the development of mRNA vaccines against bacterial and parasitic infections has lagged behind those targeting viruses. This review highlights recent studies on mRNA vaccine development and applications against a wide range of infectious diseases including non-COVID viral infections, bacterial pathogens such as Mycobacteria or Pseudomonas aeruginosa and parasitic infections, including malaria. Moreover, it discusses key optimisation strategies and highlights candidates that have progressed to clinical trials, and the current challenges in enhancing immunogenicity and improving delivery systems.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70212","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the Potential of mRNA Vaccines Against Infectious Diseases 利用mRNA疫苗对抗传染病的潜力

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-25 DOI: 10.1111/1751-7915.70212

Nouran Rezk, Siobhán McClean

{"title":"Harnessing the Potential of mRNA Vaccines Against Infectious Diseases","authors":"Nouran Rezk, Siobhán McClean","doi":"10.1111/1751-7915.70212","DOIUrl":"10.1111/1751-7915.70212","url":null,"abstract":"mRNA vaccines have emerged as promising alternatives to conventional vaccines because of their flexible design, high immunogenicity, favourable safety profile, efficacy and potential for rapid clinical development. The accelerated development of mRNA vaccines during the COVID-19 pandemic has revolutionised the field of vaccinology, highlighting their potential for combating emerging infectious diseases. The mRNA platforms can induce robust humoral as well as CD4+ and CD8+ T-cell-mediated immunity, offering broader protection than subunit protein vaccines. Consequently, they have been extensively studied against a wide range of viral, bacterial and parasitic infections, although the development of mRNA vaccines against bacterial and parasitic infections has lagged behind those targeting viruses. This review highlights recent studies on mRNA vaccine development and applications against a wide range of infectious diseases including non-COVID viral infections, bacterial pathogens such as Mycobacteria or Pseudomonas aeruginosa and parasitic infections, including malaria. Moreover, it discusses key optimisation strategies and highlights candidates that have progressed to clinical trials, and the current challenges in enhancing immunogenicity and improving delivery systems.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70212","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the Synthetic Potential of Yarrowia lipolytica: Innovating Gene Expression Tools 解脂耶氏菌的合成潜力：创新基因表达工具

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-22 DOI: 10.1111/1751-7915.70185

Xiaoqin Liu, Qingsheng Qi

引用次数: 0

Unlocking the Synthetic Potential of Yarrowia lipolytica: Innovating Gene Expression Tools 解脂耶氏菌的合成潜力：创新基因表达工具

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-22 DOI: 10.1111/1751-7915.70185

Xiaoqin Liu, Qingsheng Qi

引用次数: 0

Polymicrobial Biofilms: Interkingdom Interactions, Resistance and Therapeutic Strategies 多微生物生物膜：界间相互作用、耐药性和治疗策略

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-22 DOI: 10.1111/1751-7915.70218

Paramasivam Nithyanand, Bharath Reddy Boya, Jin-Hyung Lee, Jintae Lee

{"title":"Polymicrobial Biofilms: Interkingdom Interactions, Resistance and Therapeutic Strategies","authors":"Paramasivam Nithyanand, Bharath Reddy Boya, Jin-Hyung Lee, Jintae Lee","doi":"10.1111/1751-7915.70218","DOIUrl":"10.1111/1751-7915.70218","url":null,"abstract":"Polymicrobial biofilms are a conglomeration of diverse microbial consortia encased in a self-produced exopolysaccharide layer that forms on any biotic or abiotic surface. They are more resilient and persistent due to their enhanced drug resistance compared to monospecies biofilms, making it more difficult to eliminate using standard antimicrobial therapies. The present review discusses various inter- and intra-kingdom interactions taking place in polymicrobial biofilms and accounts for the various underlying drug resistance mechanisms in this complex and heterogeneous niche. In addition, this review provides insights into developing new diagnostic approaches by exploiting metabolites and byproducts produced by drug-resistant pathogens and other microorganisms in polymicrobial biofilms. As drug resistance is an ever-evolving mechanism in polymicrobial biofilms, synergistic combinations of natural products and antibiotics alone are not a panacea for eradicating these drug-resistant polymicrobial biofilms. Therefore, this review summarises both chemical and physical measures undertaken to combat these drug-resistant biofilms and stresses the need to employ ‘omics’ approaches, gene editing technologies and the integration of artificial intelligence/machine learning tools as future perspectives to eradicate these complex biofilms.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymicrobial Biofilms: Interkingdom Interactions, Resistance and Therapeutic Strategies 多微生物生物膜：界间相互作用、耐药性和治疗策略

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-22 DOI: 10.1111/1751-7915.70218

Paramasivam Nithyanand, Bharath Reddy Boya, Jin-Hyung Lee, Jintae Lee

{"title":"Polymicrobial Biofilms: Interkingdom Interactions, Resistance and Therapeutic Strategies","authors":"Paramasivam Nithyanand, Bharath Reddy Boya, Jin-Hyung Lee, Jintae Lee","doi":"10.1111/1751-7915.70218","DOIUrl":"https://doi.org/10.1111/1751-7915.70218","url":null,"abstract":"Polymicrobial biofilms are a conglomeration of diverse microbial consortia encased in a self-produced exopolysaccharide layer that forms on any biotic or abiotic surface. They are more resilient and persistent due to their enhanced drug resistance compared to monospecies biofilms, making it more difficult to eliminate using standard antimicrobial therapies. The present review discusses various inter- and intra-kingdom interactions taking place in polymicrobial biofilms and accounts for the various underlying drug resistance mechanisms in this complex and heterogeneous niche. In addition, this review provides insights into developing new diagnostic approaches by exploiting metabolites and byproducts produced by drug-resistant pathogens and other microorganisms in polymicrobial biofilms. As drug resistance is an ever-evolving mechanism in polymicrobial biofilms, synergistic combinations of natural products and antibiotics alone are not a panacea for eradicating these drug-resistant polymicrobial biofilms. Therefore, this review summarises both chemical and physical measures undertaken to combat these drug-resistant biofilms and stresses the need to employ ‘omics’ approaches, gene editing technologies and the integration of artificial intelligence/machine learning tools as future perspectives to eradicate these complex biofilms.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the Petri Dish: Exhibitions as Catalysts for Microbial Literacy—Bridging Science, Culture and Society 超越培养皿：展览作为微生物素养的催化剂-桥梁科学，文化和社会

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-20 DOI: 10.1111/1751-7915.70222

Rachel Armstrong

{"title":"Beyond the Petri Dish: Exhibitions as Catalysts for Microbial Literacy—Bridging Science, Culture and Society","authors":"Rachel Armstrong","doi":"10.1111/1751-7915.70222","DOIUrl":"https://doi.org/10.1111/1751-7915.70222","url":null,"abstract":"Microbes orchestrate Earth's biosphere, yet public understanding of their essential role in sustainability, health and social equity remains limited. Traditional microbiology education often fails to engage diverse audiences, perpetuating gaps in societal decision-making. This opinion piece argues for expanded microbial literacy through interdisciplinary, experiential learning, with exhibitions proposed as critical platforms to bridge science, culture and society. Drawing on the International Microbiology Literacy Initiative (IMiLI) mission, the contributions of spatial and narrative-driven encounters are considered—from ancient memory palaces to modern theatres of microbial activity—in transforming otherwise abstract microbial processes into tangible, transferrable, actionable knowledge. Individual case studies of historic and contemporary exhibitions such as We the Bacteria: Notes Toward Biotic Architecture are examined through a curatorial vision to understand how the relationship between people and microbes can be shaped through experiential knowledge while advancing microbial literacy. However, such initiatives require careful balancing of innovation with ethical communication to avoid reductive or misleading narratives. Scaling these approaches through global collaboration between scientists, educators and designers—aligned with IMiLI's vision of lifelong, learner-centric microbiology education—could effectively engage audiences who have limited access to scientific knowledge, resources, or engagement opportunities and support progress toward UN Sustainable Development Goals.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70222","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas aeruginosa Performs Chemotaxis to All Major Human Neurotransmitters 铜绿假单胞菌对所有主要的人类神经递质具有趋化作用

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-20 DOI: 10.1111/1751-7915.70211

Elizabet Monteagudo-Cascales, Miguel A. Matilla, Zulema Udaondo, José A. Gavira, Tino Krell

{"title":"Pseudomonas aeruginosa Performs Chemotaxis to All Major Human Neurotransmitters","authors":"Elizabet Monteagudo-Cascales, Miguel A. Matilla, Zulema Udaondo, José A. Gavira, Tino Krell","doi":"10.1111/1751-7915.70211","DOIUrl":"10.1111/1751-7915.70211","url":null,"abstract":"The ubiquitous pathogen Pseudomonas aeruginosa is attracted to γ-aminobutyrate (GABA), acetylcholine, histamine, serotonin, epinephrine, norepinephrine, dopamine, tyramine, glycine, and glutamate via chemotaxis. These compounds are all major neurotransmitters in humans. They are also found in various non-neuronal tissues and are synthesised by different organisms, including bacteria, protozoa, invertebrates, and plants. Many of these neurotransmitters increase the expression of virulence-related genes in P. aeruginosa, so that chemotaxis to these compounds may constitute an important virulence factor. The chemotactic response is initiated by the direct binding of these compounds to the dCache ligand-binding domains of the PctC, TlpQ, PctD, PctA, and PctB chemoreceptors. Previous studies have shown that Escherichia coli is attracted to epinephrine, norepinephrine, and dopamine. These responses are mediated by the Tar and Tsr chemoreceptors, which possess four-helix bundle-type ligand-binding domains. The use of structurally dissimilar chemoreceptors to mediate neurotransmitter chemotaxis suggests convergent evolution. This article is intended to stimulate the study of the connection between neurotransmitter chemotaxis and virulence in P. aeruginosa and to expand the search for neurotransmitter chemotaxis in other motile bacteria.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the Petri Dish: Exhibitions as Catalysts for Microbial Literacy—Bridging Science, Culture and Society 超越培养皿：展览作为微生物素养的催化剂-桥梁科学，文化和社会

IF 5.2 2区生物学

Microbial Biotechnology Pub Date : 2025-08-20 DOI: 10.1111/1751-7915.70222

Rachel Armstrong

{"title":"Beyond the Petri Dish: Exhibitions as Catalysts for Microbial Literacy—Bridging Science, Culture and Society","authors":"Rachel Armstrong","doi":"10.1111/1751-7915.70222","DOIUrl":"10.1111/1751-7915.70222","url":null,"abstract":"Microbes orchestrate Earth's biosphere, yet public understanding of their essential role in sustainability, health and social equity remains limited. Traditional microbiology education often fails to engage diverse audiences, perpetuating gaps in societal decision-making. This opinion piece argues for expanded microbial literacy through interdisciplinary, experiential learning, with exhibitions proposed as critical platforms to bridge science, culture and society. Drawing on the International Microbiology Literacy Initiative (IMiLI) mission, the contributions of spatial and narrative-driven encounters are considered—from ancient memory palaces to modern theatres of microbial activity—in transforming otherwise abstract microbial processes into tangible, transferrable, actionable knowledge. Individual case studies of historic and contemporary exhibitions such as We the Bacteria: Notes Toward Biotic Architecture are examined through a curatorial vision to understand how the relationship between people and microbes can be shaped through experiential knowledge while advancing microbial literacy. However, such initiatives require careful balancing of innovation with ethical communication to avoid reductive or misleading narratives. Scaling these approaches through global collaboration between scientists, educators and designers—aligned with IMiLI's vision of lifelong, learner-centric microbiology education—could effectively engage audiences who have limited access to scientific knowledge, resources, or engagement opportunities and support progress toward UN Sustainable Development Goals.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 8","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70222","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0