Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery

IF 5.7 2区 生物学
Ilona A. Ruhl, Sean P. Woodworth, Stefan J. Haugen, Hannah M. Alt, Gregg T. Beckham, Christopher W. Johnson
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引用次数: 0

Abstract

Vanillin is the most in-demand flavouring compound in the world and because vanillin extracted from vanilla pods cannot meet the global demand, most vanillin on the market today is chemically synthesised. Increasing demands by consumers for natural ingredients have inspired efforts to develop vanillin derived from microbial sources. These efforts have been challenged by low titers, likely caused by the toxicity of vanillin to most microbial biocatalysts. In this study, we engineered a Pseudomonas putida KT2440-derived strain that accumulated vanillin from ferulic acid to 0.64 g/L. To increase the overall titre, we applied a hydrophobic polystyrene-based resin to vanillin-accumulating cultures, which enabled an increase in total vanillin recovery to an apparent titre of 3.35 g/L. This study demonstrates that P. putida can accumulate vanillin from ferulic acid to higher titers when vanillin is removed from the cultivation medium, mitigating its toxicity.

腐臭假单胞菌KT2440利用代谢工程和原位产物回收从阿魏酸中生产香兰素
香草素是世界上需求量最大的调味化合物,由于从香草豆荚中提取的香草素无法满足全球需求,目前市场上的大多数香草素都是化学合成的。消费者对天然成分日益增长的需求激发了从微生物来源开发香兰素的努力。这些努力受到低滴度的挑战,可能是由于香草醛对大多数微生物生物催化剂的毒性造成的。在这项研究中,我们设计了一株恶臭假单胞菌kt2440衍生菌株,该菌株从阿魏酸中积累香兰素至0.64 g/L。为了提高总滴度,我们将疏水性聚苯乙烯基树脂应用于香草素积累培养物,这使得总香草素回收率增加到3.35 g/L的表观滴度。本研究表明,当从培养基中去除香兰素时,恶臭假单胞菌可以从阿魏酸中积累更高滴度的香兰素,从而减轻其毒性。
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来源期刊
Microbial Biotechnology
Microbial Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
11.20
自引率
3.50%
发文量
162
审稿时长
1 months
期刊介绍: Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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