Pseudomonas aeruginosa Performs Chemotaxis to the Neurotransmitters Serotonin, Dopamine, Epinephrine and Norepinephrine

IF 5.7 2区 生物学
Elizabet Monteagudo-Cascales, Andrea Lozano-Montoya, Tino Krell
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引用次数: 0

Abstract

Bacteria use chemotaxis to move to favourable ecological niches. For many pathogenic bacteria, chemotaxis is required for full virulence, particularly for the initiation of host colonisation. There do not appear to be limits to the type of compounds that attract bacteria, and we are just beginning to understand how chemotaxis adapts them to their lifestyles. Quantitative capillary assays for chemotaxis show that P. aeruginosa is strongly attracted to serotonin, dopamine, epinephrine and norepinephrine. Chemotaxis to these compounds is greatly decreased in a mutant lacking the TlpQ chemoreceptor, and complementation of this mutant with a plasmid harbouring the tlpQ gene restores wild-type-like chemotaxis. Microcalorimetric titrations of the TlpQ sensor domain with these four compounds indicate that they bind directly to TlpQ. All four compounds are hormones and neurotransmitters that control a variety of processes and are also important signal molecules involved in the virulence of P. aeruginosa. They modulate motility, biofilm formation, the production of virulence factors and serve as siderophores that chelate iron. Additionally, this is the first report of bacterial chemotaxis to serotonin. This study provides an incentive for research to define the contribution of chemotaxis to these host signalling molecules to the virulence of P. aeruginosa.

铜绿假单胞菌对神经递质5 -羟色胺、多巴胺、肾上腺素和去甲肾上腺素具有趋化作用
细菌利用趋化作用向有利的生态位移动。对于许多致病菌来说,趋化性是完全毒力所必需的,特别是在开始宿主定植时。吸引细菌的化合物类型似乎没有限制,我们刚刚开始了解趋化性是如何使它们适应它们的生活方式的。化学趋向性的定量毛细管分析表明,铜绿假单胞菌对血清素、多巴胺、肾上腺素和去甲肾上腺素具有强烈的吸引力。在缺乏TlpQ趋化受体的突变体中,对这些化合物的趋化性大大降低,并且将该突变体与含有TlpQ基因的质粒互补可以恢复野生型的趋化性。用这四种化合物对TlpQ传感器结构域进行微量热滴定,表明它们直接与TlpQ结合。这四种化合物都是控制多种过程的激素和神经递质,也是铜绿假单胞菌毒力的重要信号分子。它们调节运动、生物膜的形成、毒力因子的产生,并作为铁载体螯合铁。此外,这是第一次报道细菌对血清素的趋化性。这项研究为研究这些宿主信号分子的趋化性对铜绿假单胞菌毒力的贡献提供了动力。
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来源期刊
Microbial Biotechnology
Microbial Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
11.20
自引率
3.50%
发文量
162
审稿时长
1 months
期刊介绍: Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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