刚地弓形虫C2结构域蛋白缺失突变体作为抗小鼠弓形虫病疫苗的前景

IF 5.7 2区 生物学
Yifan Luo, Mingfeng He, Shengqiang Yang, Jiahui Qian, Zhengming He, Jiayin Xu, Liyu Guo, Siyu Xiao, Rui Fang
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引用次数: 0

摘要

刚地弓形虫(弓形虫)是一种能够感染几乎所有温血动物的寄生原生动物,对牲畜造成重大经济损失,并对动物和公众健康构成重大威胁。尽管它有影响,但目前还没有理想的疫苗来预防弓形虫病。囊泡运输在弓形虫的生命周期中起着至关重要的作用,参与这一过程的蛋白质——例如含有C2结构域的蛋白质——可能成为开发减毒活疫苗的新靶点。在这项研究中,我们评估了含C2结构域蛋白(TGME49_203240)作为减毒活疫苗候选物的可行性。我们的研究结果表明TGME49_203240可能参与囊泡运输,并且对弓形虫的生长至关重要。TGME49_203240的缺失降低了小鼠体内寄生虫的毒力并破坏了组织囊肿的形成。此外,接种ME49Δ203240的小鼠对弓形虫I、II、III株速殖子和II株囊的致命攻击具有保护作用。此外,ME49Δ203240菌株引发了强大的免疫应答,包括产生高水平的特异性IgG抗体和关键细胞因子(IFN-γ, TNF-α和IL-12)。这些发现强调TGME49_203240是开发弓形虫减毒活疫苗的一个有希望的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Toxoplasma gondii C2 Domain Protein Deletion Mutant as a Promising Vaccine Against Toxoplasmosis in Mice

Toxoplasma gondii (T. gondii), a parasitic protozoan capable of infecting nearly all warm-blooded animals, causes significant economic losses in livestock and poses a significant threat to both animal and public health. Despite its impact, no ideal vaccine is currently available to prevent toxoplasmosis. Vesicular transport plays a crucial role in the life cycle of T. gondii, and proteins involved in this process – such as those containing C2 domains – may serve as novel targets for the development of live attenuated vaccines. In this study, we evaluated the feasibility of a C2 domain-containing protein (TGME49_203240) as a live attenuated vaccine candidate. Our findings suggest that TGME49_203240 may be involved in vesicular transport and that it is essential for T. gondii growth. Deletion of TGME49_203240 reduced parasite virulence and impaired tissue cyst formation in mice. Moreover, mice vaccinated with ME49Δ203240 were protected against the lethal challenge of the tachyzoites of T. gondii I, II, III strains and cysts of II strain. In addition, the ME49Δ203240 strain elicited robust immune responses, including the production of high levels of specific IgG antibodies and key cytokines (IFN-γ, TNF-α and IL-12). These findings highlight TGME49_203240 as a promising target for the development of a live attenuated vaccine against T. gondii.

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来源期刊
Microbial Biotechnology
Microbial Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
11.20
自引率
3.50%
发文量
162
审稿时长
1 months
期刊介绍: Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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