Microbial Biotechnology最新文献_第4页

Living Engineered Bacterial Therapeutics: Emerging Affordable Precision Interventions 活体工程细菌疗法：新兴的可负担得起的精准干预。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-23 DOI: 10.1111/1751-7915.70057

Rajkamal Srivastava, Cammie F. Lesser

引用次数: 0

Microalgae and cyanobacteria as microbial substrate and their influence on the potential postbiotic capability of a bacterial probiotic 微藻和蓝藻作为微生物底物及其对细菌益生菌潜在后生能力的影响。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-21 DOI: 10.1111/1751-7915.70046

Marta Domínguez-Maqueda, Olivia Pérez-Gómez, Jorge García-Márquez, Cristóbal Espinosa-Ruíz, Alberto Cuesta, Mª. Ángeles Esteban, Francisco Javier Alarcón-López, Casimiro Cárdenas, Silvana T. Tapia-Paniagua, Mª. Carmen Balebona, Miguel Ángel Moriñigo

{"title":"Microalgae and cyanobacteria as microbial substrate and their influence on the potential postbiotic capability of a bacterial probiotic","authors":"Marta Domínguez-Maqueda, Olivia Pérez-Gómez, Jorge García-Márquez, Cristóbal Espinosa-Ruíz, Alberto Cuesta, Mª. Ángeles Esteban, Francisco Javier Alarcón-López, Casimiro Cárdenas, Silvana T. Tapia-Paniagua, Mª. Carmen Balebona, Miguel Ángel Moriñigo","doi":"10.1111/1751-7915.70046","DOIUrl":"10.1111/1751-7915.70046","url":null,"abstract":"Postbiotics are metabolic by-products from microorganisms that provide health benefits to the host. Their secretion can be influenced by various conditions affecting bacterial metabolism. This study presents a novel approach for producing potential postbiotics, specifically extracellular products (ECPs), from the probiotic strain Shewanella putrefaciens SpPdp11, grown under different culture conditions. These conditions include aquafeed media, with partial or total microalgae/cyanobacteria replacement as the microbial substrate, as well as variations in temperature and growth phase. The use of microalgae/cyanobacteria as substrates may represent a valuable strategy for generating novel postbiotics with unique properties. The ECPs assessed were evaluated for their in vitro cytotoxic, hydrolytic and antimicrobial activities. Three conditions (ECPs derived from aquafeed media with partial (FM2324 and FM1548) or total (M2324) microalgae/cyanobacteria replacement) were non-cytotoxic to various fish cell lines and hydrolysed key nutritional compounds (casein, lipids, amylase and gelatin). Proteomic analysis of these ECP conditions revealed common structural and regulatory DNA-associated proteins, while differentially expressed proteins were associated with amino acid metabolism and antioxidant system (FM2324 and FM1548) and chemotaxis system (M2324). The results highlight the potential of the selected postbiotics as feed additives for future in vivo studies, aligning with sustainable development for aquaculture.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 11","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of fleQ Deficiency on Resource Allocation and Heterologous Gene Expression in Pseudomonas putida Across Various Growth Media 不同生长介质中 fleQ 缺乏对假单胞菌资源分配和异源基因表达的影响

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-21 DOI: 10.1111/1751-7915.70054

Junyoung Kim, Sooyeon Lee, Alexander P. S. Darlington, Juhyun Kim

{"title":"Impact of fleQ Deficiency on Resource Allocation and Heterologous Gene Expression in Pseudomonas putida Across Various Growth Media","authors":"Junyoung Kim, Sooyeon Lee, Alexander P. S. Darlington, Juhyun Kim","doi":"10.1111/1751-7915.70054","DOIUrl":"10.1111/1751-7915.70054","url":null,"abstract":"Pseudomonas putida is widely used in industrial applications, including the recombinant proteins production, because of its natural advantageous properties. In this study, the gene encoding FleQ, the primary regulator of flagellar synthesis, was deleted to construct a new non-motile P. putida KT2440-derived strain (ΔfleQ). The non-motile cells showed reduced biofilm formation and enhanced expression of a heterologous gene in nutrient-rich media compared with the wild-type (WT) strain, attributed to the reallocation of cellular resources from flagellar synthesis and cellular motility. Additionally, the ΔfleQ strain exhibited enhanced tolerance to chloramphenicol, indicating higher ribosome production, confirmed by a higher RNA/protein ratio relative to the WT. While the WT strain showed decreased growth and a three-fold increase in reporter gene activity in minimal media, the ΔfleQ strain maintained consistent reporter gene expression and exhibited a relatively higher growth rate. This suggests that the FleQ is involved in modulating proteome allocation based on nutrient quality. The removal of FleQ allows for more flexible resource allocation, creating a chassis strain with nutrient quality-independent gene expression capacity, which could be valuable in industrial applications where consistent output is essential.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 11","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha-4,11-Diene Production 在保持性能的同时替代酵母提取物：展示 Amorpha-4,11-二烯的生产。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-21 DOI: 10.1111/1751-7915.70056

Carlos Castillo-Saldarriaga, Christine N. S. Santos, Stephen Sarria, Parayil K. Ajikumar, Ralf Takors

{"title":"Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha-4,11-Diene Production","authors":"Carlos Castillo-Saldarriaga, Christine N. S. Santos, Stephen Sarria, Parayil K. Ajikumar, Ralf Takors","doi":"10.1111/1751-7915.70056","DOIUrl":"10.1111/1751-7915.70056","url":null,"abstract":"Yeast extract (YE) is a complex nutritional source associated with high performance on microbial production processes. However, its inherent compositional variability challenges its scalability. While prior efforts have focused on growth-associated products, the dynamics of growth-uncoupled production, which leads to higher production rates and conversion yields, still need to be explored. This production scenario is common in large-scale applications. This study presents a systematic approach to replace YE for the production of the terpene amorpha-4,11-diene in Escherichia coli. Sequential processing was successfully applied to identify glutamic acid, alanine, leucine, valine, isoleucine and glycine as the key amino acids (AAs) under slow-growth conditions. Thoroughly applying biomass retention as part of sequential processing increased production capacity by 45% using these AAs instead of YE. Further studies, including flux balance analyses, targeted pyruvate as the common AA precursor. The optimized fed-batch process feeding pyruvate with 0.09 gPyr h−1 enhanced amorpha-4,11-diene production by 37%, although adding only 1% carbon via pyruvate. Flux balance analysis revealed the criteria for optimum pyruvate feeding, for example, to prevent succinate secretion and maintain the NADH/NAD+ balance. These findings illustrate the interplay between media composition and metabolic activity and provide a successful guideline for identifying lean, best-performing media for industrial applications.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 11","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined oxygen and glucose oscillations distinctly change the transcriptional and physiological state of Escherichia coli 氧和葡萄糖的联合振荡明显改变了大肠杆菌的转录和生理状态。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-15 DOI: 10.1111/1751-7915.70051

Jonas Bafna-Rührer, Jean V. Orth, Suresh Sudarsan

{"title":"Combined oxygen and glucose oscillations distinctly change the transcriptional and physiological state of Escherichia coli","authors":"Jonas Bafna-Rührer, Jean V. Orth, Suresh Sudarsan","doi":"10.1111/1751-7915.70051","DOIUrl":"10.1111/1751-7915.70051","url":null,"abstract":"Escherichia coli, a common microbial host for industrial bioproduction, experiences a highly dynamic environment in industrial-scale bioreactors due to significant glucose and oxygen gradients. In this study, we mimic the combined gradients of glucose and oxygen in high-throughput bioreactors to study the transcriptional response of E. coli to industrial-scale conditions. Under oscillating oxygen conditions, E. coli formed less biomass and accumulated the anaerobic by-product acetate. With respect to oxygen-responsive genes, we found that genes of the TCA cycle and of different electron transport chain complexes were differentially expressed. A global analysis of the expression data revealed that oxygen oscillations had caused a transition towards a catabolite-repressed state and upregulation of several stress-related regulatory programs. Interestingly, the transcriptional changes persisted after oxygen limitation stopped. In contrast, the changes we observed due to glucose starvation, such as induction of the stringent response, were primarily transient. Most importantly, we found that effects of combined oxygen and glucose oscillations were distinct from the ones of oxygen and substrate oscillations alone, suggesting an important interplay between the different metabolic regimes in industrial-scale bioreactors.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 11","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights for the development of efficient DNA vaccines 开发高效 DNA 疫苗的新见解。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-15 DOI: 10.1111/1751-7915.70053

Simone Berger, Yanira Zeyn, Ernst Wagner, Matthias Bros

{"title":"New insights for the development of efficient DNA vaccines","authors":"Simone Berger, Yanira Zeyn, Ernst Wagner, Matthias Bros","doi":"10.1111/1751-7915.70053","DOIUrl":"10.1111/1751-7915.70053","url":null,"abstract":"Despite the great potential of DNA vaccines for a broad range of applications, ranging from prevention of infections, over treatment of autoimmune and allergic diseases to cancer immunotherapies, the implementation of such therapies for clinical treatment is far behind the expectations up to now. The main reason is the poor immunogenicity of DNA vaccines in humans. Consequently, the improvement of the performance of DNA vaccines in vivo is required. This mini-review provides an overview of the current state of DNA vaccines and the various strategies to enhance the immunogenic potential of DNA vaccines, including (i) the optimization of the DNA construct itself regarding size, nuclear transfer and transcriptional regulation; (ii) the use of appropriate adjuvants; and (iii) improved delivery, for example, by careful choice of the administration route, physical methods such as electroporation and nanomaterials that may allow cell type-specific targeting. Moreover, combining nanoformulated DNA vaccines with other immunotherapies and prime-boost strategies may help to enhance success of treatment.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 11","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Catabolism of Phthalates With Estrogenic Activity Used as Plasticisers in the Manufacture of Plastic Products 在塑料制品生产中用作增塑剂的具有雌激素活性的邻苯二甲酸盐的细菌分解作用。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-15 DOI: 10.1111/1751-7915.70055

Rinita Dhar, Suman Basu, Mousumi Bhattacharyya, Debarun Acharya, Tapan K. Dutta

{"title":"Bacterial Catabolism of Phthalates With Estrogenic Activity Used as Plasticisers in the Manufacture of Plastic Products","authors":"Rinita Dhar, Suman Basu, Mousumi Bhattacharyya, Debarun Acharya, Tapan K. Dutta","doi":"10.1111/1751-7915.70055","DOIUrl":"10.1111/1751-7915.70055","url":null,"abstract":"Phthalic acid esters (PAEs), the pervasive and ubiquitous endocrine-disrupting chemicals of environmental concern, generated annually on a million-ton scale, are primarily employed as plasticisers in the production of a variety of plastic products and as additives in a large number of commercial supplies. The increased awareness of various adverse effects on the ecosystem and human health including reproductive and developmental disorders has led to a striking increase in research interest aimed at managing these man-made oestrogenic chemicals. In these circumstances, microbial metabolism appeared as the major realistic process to neutralise the toxic burdens of PAEs in an ecologically accepted manner. Among a wide variety of microbial species capable of degrading/transforming PAEs reported so far, bacteria-mediated degradation has been studied most extensively. The main purpose of this review is to provide current knowledge of metabolic imprints of microbial degradation/transformation of PAEs, a co-contaminant of plastic pollution. In addition, this communication illustrates the recent advancement of the structure–functional aspects of the key metabolic enzyme phthalate hydrolase, their inducible regulation of gene expression and evolutionary relatedness, besides prioritising future research needs to facilitate the development of new insights into the bioremediation of PAE in the environment.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 11","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, potential and limitations of conjugation-based antibacterial strategies 基于共轭的抗菌策略的设计、潜力和局限性。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-15 DOI: 10.1111/1751-7915.70050

Elisabeth Derollez, Christian Lesterlin, Sarah Bigot

引用次数: 0

Microbial biosensors for diagnostics, surveillance and epidemiology: Today's achievements and tomorrow's prospects 用于诊断、监测和流行病学的微生物生物传感器：今天的成就和明天的前景。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-15 DOI: 10.1111/1751-7915.70047

Julien Capin, Emile Chabert, Ana Zuñiga, Jerome Bonnet

引用次数: 0

Phage biocontrol success of bacterial wilt depends on synergistic interactions with resident rhizosphere microbiota 噬菌体生物防治细菌枯萎病的成功取决于与根瘤菌群的协同作用。

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2024-11-13 DOI: 10.1111/1751-7915.70049

Sara Franco Ortega, Bryden Fields, Daniel Narino Rojas, Lauri Mikonranta, Matthew Holmes, Andrea L. Harper, Ville-Petri Friman

{"title":"Phage biocontrol success of bacterial wilt depends on synergistic interactions with resident rhizosphere microbiota","authors":"Sara Franco Ortega, Bryden Fields, Daniel Narino Rojas, Lauri Mikonranta, Matthew Holmes, Andrea L. Harper, Ville-Petri Friman","doi":"10.1111/1751-7915.70049","DOIUrl":"10.1111/1751-7915.70049","url":null,"abstract":"Phages can successfully be used in vitro and in planta to biocontrol the phytopathogenic Ralstonia solanacearum bacterium—the causal agent of bacterial wilt disease. However, phage biocontrol outcomes are still variable, and it is unclear what causes this. In this study, we assessed the efficiency of four phages in controlled in vitro and in planta experiments in all one- and two-phage combinations. We found that using phages in combination did not improve the phage biocontrol efficiency relative to single phage treatments, while certain phages and their combinations were more effective than the others. High intra-treatment variability in phage efficiency was observed across all phage treatments, which was associated with clear shifts in microbiome composition, a reduction in R. solanacearum and an increase in phage densities. We further identified the bacterial taxa that were associated with these ‘shifted’ microbiomes and conducted additional plant growth experiments, demonstrating that some of the enriched bacterial species could protect plants from R. solanacearum infections—a pattern which was also observed using partial least squares path modelling (PLS-PM). Together, these results suggest that phages could open niche space for beneficial bacteria by reducing pathogen densities and that variability in phage biocontrol outcomes is rhizosphere microbiome-dependent, which can introduce between-replicate variation, even in controlled greenhouse conditions.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"17 11","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0