Microbial Biotechnology最新文献_第3页

Rapid Identification and Typing of Carbapenem-Resistant Klebsiella pneumoniae Using MALDI-TOF MS and Machine Learning 利用MALDI-TOF质谱和机器学习快速鉴定耐碳青霉烯肺炎克雷伯菌

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-16 DOI: 10.1111/1751-7915.70184

Zhiyi Ye, Jin Zhu, Yang Liu, Jun Lu

{"title":"Rapid Identification and Typing of Carbapenem-Resistant Klebsiella pneumoniae Using MALDI-TOF MS and Machine Learning","authors":"Zhiyi Ye, Jin Zhu, Yang Liu, Jun Lu","doi":"10.1111/1751-7915.70184","DOIUrl":"https://doi.org/10.1111/1751-7915.70184","url":null,"abstract":"Use matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) to screen the specific mass peaks of carbapenem-resistant Klebsiella pneumoniae (CRKP), compare the differences in spectrum peaks between intestinal and bloodstream screening of CRKP, and assess the utility of MALDI-TOF MS in quickly identifying various CRKP sources. From 2014 to 2023, a total of 267 Klebsiella pneumoniae strains were collected at Quzhou People's Hospital, including 60 intestinal screening isolates from ICU patients and 207 bloodstream infection isolates. MALDI-TOF MS was used to profile peptides in CRKP and carbapenem-sensitive Klebsiella pneumoniae (CSKP), followed by analysis with flexAnalysis and ClinProTools 3.0. Statistically significant protein peaks were selected to build classification models, which were verified using non-duplicate strains. MALDI-TOF MS achieved > 99.9% accuracy in identifying Klebsiella pneumoniae. Characteristic peaks (2523.43, 3041.62, 4520.11, 10,079.18 Da) were used to develop resistance analysis models, with the optimal model (SNN) showing 90.08% sensitivity, 95.80% specificity and identification accuracies of 90% for CSKP and 89.66% for CRKP. Another model using peaks (8876, 8993, 9139 Da) differentiated CRKP origins, with the ideal model (QC) achieving 86.85% sensitivity, 88.46% specificity, and accuracies of 81.82% for bloodstream and 95.00% for intestinal CRKP.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PFAS Biodegradation and the Constraints of Thermodynamics PFAS的生物降解及其热力学约束

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-16 DOI: 10.1111/1751-7915.70181

Lawrence P. Wackett

引用次数: 0

Indole Derivatives Biosynthesis in Bifidobacterium longum subsp. infantis and the Tryptophan Substrate Availability 长双歧杆菌中吲哚衍生物的生物合成。婴儿和色氨酸底物有效性

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-16 DOI: 10.1111/1751-7915.70167

Shi-Min Zhang, Hui-Chu Wu, Jia-He Hung, Shir-Ly Huang

{"title":"Indole Derivatives Biosynthesis in Bifidobacterium longum subsp. infantis and the Tryptophan Substrate Availability","authors":"Shi-Min Zhang, Hui-Chu Wu, Jia-He Hung, Shir-Ly Huang","doi":"10.1111/1751-7915.70167","DOIUrl":"https://doi.org/10.1111/1751-7915.70167","url":null,"abstract":"The metabolic processes of Bifidobacterium longum subsp. infantis, an early coloniser of the human gut, are essential for gut health, mainly due to the production of indole derivatives from tryptophan. This study investigates the capacity of B. infantis ATCC 15697 to biosynthesise indole-3-lactate (ILA), indole-3-acetate (IAA), and indole-3-carboxaldehyde (I3CA) and the regulatory effects of substrate availability on these pathways. The tryptophan catabolic profile of B. infantis ATCC 15697 under a non-growing but metabolically active state was investigated. Through HPLC-PDA and LC–MS analyses, we confirmed for the first time the production of IAA and I3CA by B. infantis ATCC 15697. The results revealed a dose-dependent relationship between tryptophan availability and the production of indole derivatives, highlighting the nutrient-driven effect of these metabolic pathways. By integrating genomic analysis with metabolic profiles, we proposed potential pathways underlying the biosynthesis of IAA and I3CA from tryptophan. These findings enhance our understanding of the role of B. infantis ATCC 15697 in human health, with ILA, IAA, and I3CA contributing to immune modulation and gut health. We also provide a platform for using B. infantis ATCC 15697 as a biocatalyst for the biosynthesis of beneficial indole derivatives through whole-cell bioconversion, which was further demonstrated in B. infantis ATCC 25962 and ATCC 15702. Future in vivo studies will help clarify the impact of these metabolites on the gut environment and inform dietary and probiotic strategies for enhancing indole derivatives production.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Public Health Microbiome Curriculum: Looking Below the Tip of the Iceberg for Approaches to Population Health 公共卫生微生物组课程：在人口健康方法的冰山一角下面看

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-14 DOI: 10.1111/1751-7915.70160

Melissa K. Melby, Swapna Mylabathula, Meghan B. Azad, Sarah Turner, Naama Geva-Zatorsky, Carolina Tropini, Melissa B. Manus, Martin Blaser, Mark Nichter

{"title":"Public Health Microbiome Curriculum: Looking Below the Tip of the Iceberg for Approaches to Population Health","authors":"Melissa K. Melby, Swapna Mylabathula, Meghan B. Azad, Sarah Turner, Naama Geva-Zatorsky, Carolina Tropini, Melissa B. Manus, Martin Blaser, Mark Nichter","doi":"10.1111/1751-7915.70160","DOIUrl":"https://doi.org/10.1111/1751-7915.70160","url":null,"abstract":"We discuss the opportunity for public health microbiome curricula to bridge the gaps in knowledge that exist between microbiome researchers and the lay public. We propose equipping public health professionals, important facilitators of public outreach and behaviour change, with three public health curriculum modules focused on breastfeeding, antibiotics and diet. These modules shift the focus from microbes as pathogens to potential partners in promoting health across the life course. Current public health messages cover only the ‘tip of the iceberg’ in exploring mechanisms, and this microbiome curriculum dives below the surface to provide fresh perspectives. These microbiome insights allow us to move beyond a focus on microbes as pathogens to understand the numerous collaborative roles played by the microbiome in producing health, and the upstream factors influencing the microbiome, thereby offering mechanistic insights that can be harnessed for public health education.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic-Based Approaches for Sustainable Control of Infectious Risk in Mass Transport: Current Data and Future Perspectives 基于益生菌的公共交通感染风险可持续控制方法：当前数据和未来展望

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-14 DOI: 10.1111/1751-7915.70177

Irene Soffritti, Maria D'Accolti, Francesca Bini, Eleonora Mazziga, Antonella Volta, Matteo Bisi, Sante Mazzacane, Elisabetta Caselli

{"title":"Probiotic-Based Approaches for Sustainable Control of Infectious Risk in Mass Transport: Current Data and Future Perspectives","authors":"Irene Soffritti, Maria D'Accolti, Francesca Bini, Eleonora Mazziga, Antonella Volta, Matteo Bisi, Sante Mazzacane, Elisabetta Caselli","doi":"10.1111/1751-7915.70177","DOIUrl":"https://doi.org/10.1111/1751-7915.70177","url":null,"abstract":"The built environments of high-traffic areas can play a significant role in the transmission of microorganisms and associated infections, sometimes favouring the selection of multidrug-resistant (MDR) organisms due to the excessive use of conventional disinfectants. Probiotic-based sanitation (PBS) was suggested as a novel alternative approach to control the infectious risk in crowded community environments due to its effectiveness in reducing fungal, bacterial, and viral pathogens in sanitary settings. PBS may thus trigger a paradigm shift from chemical to biological strategies in cleaning environments with high human occupancy, offering an ecological and economically sustainable alternative to conventional chemical disinfection. Providing robust data supporting the results reported so far, it has the potential to optimise bioburden control and infection prevention in mass transportation spaces. This review brings together existing research on PBS in mass transportation areas, pinpoints areas of lack of information, and explores its potential future uses, including the creation of probiotic-based materials for sustainable biocontrol in high-traffic areas.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70177","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methanol-Free Protein Expression in Komagataella phaffii With Magnetic or Non-Magnetic Heating 磁加热和非磁加热法菲黑马藻无甲醇蛋白的表达

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-14 DOI: 10.1111/1751-7915.70183

Ibrahim Dagci, Seyda Yildiz Arslan, Kubra Solak, Melek Acar, Yagmur Unver, Ahmet Mavi

{"title":"Methanol-Free Protein Expression in Komagataella phaffii With Magnetic or Non-Magnetic Heating","authors":"Ibrahim Dagci, Seyda Yildiz Arslan, Kubra Solak, Melek Acar, Yagmur Unver, Ahmet Mavi","doi":"10.1111/1751-7915.70183","DOIUrl":"https://doi.org/10.1111/1751-7915.70183","url":null,"abstract":"Komagataella phaffii is among the most widely used expression systems, with methanol-inducible promoters being preferred for protein expression due to their stringent regulation and exceptional strength. However, the applicability of this system, particularly in food and pharmaceutical products, is limited by methanol's toxic and pro-inflammatory properties. Therefore, obtaining a novel methanol-free expression system is necessary. In this study, we obtained a novel expression plasmid, pHSPαA, carrying the HSP70 promoter (PHSP70) to regulate heterologous expression through heat induction. The extracellular expression of azurin was achieved using this methanol-free system under the control of PHSP70, induced by either magnetic or non-magnetic heating. To enhance heat-induced expression, recombinant cells were immobilised with Fe3O4@PEI25 kDa nanoparticles, which facilitated heat release under an AC magnetic field, thereby increasing cell permeability and protein secretion. A time-dependent increase in protein expression was observed in non-magnetic heating but not under magnetic heating. However, immobilised cells exhibited a higher protein secretion capacity compared to non-immobilised cells. These findings suggest that the novel methanol-free expression system represents a promising alternative for heterologous gene expression, particularly for the production of therapeutically relevant and food-grade recombinant proteins.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revitalising Brewers' Spent Grains and Enriching With Biogenic Compounds Through the Fermentation of Fructophilic Lactic Acid Bacteria and Yeasts 通过嗜果糖乳酸菌和酵母菌的发酵活化啤酒废粮并富集生物化合物

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-09 DOI: 10.1111/1751-7915.70171

Alessandro Stringari, Ali Zein Alabiden Tlais, Stefano Tonini, Daniela Pinto, Giorgia Mondadori, Pasquale Filannino, Raffaella Di Cagno, Marco Gobbetti

{"title":"Revitalising Brewers' Spent Grains and Enriching With Biogenic Compounds Through the Fermentation of Fructophilic Lactic Acid Bacteria and Yeasts","authors":"Alessandro Stringari, Ali Zein Alabiden Tlais, Stefano Tonini, Daniela Pinto, Giorgia Mondadori, Pasquale Filannino, Raffaella Di Cagno, Marco Gobbetti","doi":"10.1111/1751-7915.70171","DOIUrl":"https://doi.org/10.1111/1751-7915.70171","url":null,"abstract":"The large output of spent grains from the brewing industry presents environmental concerns but also offers promising nutritional and functional potential for valorization by researchers and industrial stakeholders. In this perspective, we investigated how non-conventional starters like Fructobacillus fructosus PL22 and Wickerhamomyces anomalus GY1 can drive the fermentation of brewer's spent grain (BSG), a solid by-product of the brewing industry, to enrich its portfolio of bioactive compounds. While sugar reduction was comparable between started- and unstarted-BSG, the effect of the fermentation became evident through the release of key microbial metabolites (lactic and acetic acids and ethanol). Both starters generated the highest number of unique peptides, with only one previously identified as antioxidant peptide found in BSG fermented with F. fructosus. During fermentation, most amino acids and phenolic compounds decreased, while BSG fermented with W. anomalus distinctly enhanced the release of Ala, Cys and GABA, and health-promoting phenolic compounds, such as gallic acid, gallocatechin, quercetin, naringenin, kaempferol, and isorhamnetin. These metabolic changes were associated with the enhanced antifungal and antioxidant properties, which in turn positively reflected on skin protection as shown by the increased proliferation of human keratinocytes, over-expression of the filaggrin (FLG) gene, and wound healing. The power of fermentation to revitalise BSG, giving it a second life chance through the improvement of its nutritional value and further multifunctionality, was demonstrated.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70171","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting D-Acylases for D-Amino Acid Production 重新审视d -酰化酶对d -氨基酸的生产

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-09 DOI: 10.1111/1751-7915.70179

Sergio Martínez-Rodríguez, Jose Antonio Gavira

{"title":"Revisiting D-Acylases for D-Amino Acid Production","authors":"Sergio Martínez-Rodríguez, Jose Antonio Gavira","doi":"10.1111/1751-7915.70179","DOIUrl":"https://doi.org/10.1111/1751-7915.70179","url":null,"abstract":"N-Acyl-D-amino acid deacylases (EC 3.5.1.81, also known as D-acylases) have been studied for decades for their utility in the kinetic resolution of N-acetyl-D,L-amino acids (NAAs) due to a marked stereospecificity. In conjunction with an N-succinyl-amino acid racemase (NSAR), they impulse the dynamic kinetic resolution (DKR) of different NAAs until the corresponding enantiomerically pure D-amino acids. Besides the clear interest in this enzyme cascade, the application of D-acylase/NSAR tandems has been only briefly described outside the industrial field. In this work, we revisit D-acylases for the DKR of NAAs, reporting the characterisation of two new recombinant D-acylases belonging to Bordetella petrii and Klebsiella pneumoniae. The enzymes were successfully coupled with the recombinant NSAR from Geobacillus stearothermophilus for the biosynthesis of D-methionine or D-aminobutyric acid. We also carried out the structural characterisation of the D-acylase from Klebsiella pneumoniae (KleDacyl), providing the second experimental 3-D structure of a member of this family of enzymes. The structural model shows a highly dynamic character of this amidohydrolase superfamily member, supplying a snapshot of an open conformation of the enzyme most likely preceding substrate entrance into the catalytic cleft. Our results confirm for the first time the importance of an α/β mobile domain in the substrate specificity of D-acylases (region 282–341 in KleDacyl), opening up new strategies for structural-based protein engineering strategies.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary Trajectories of Methionine Metabolism in Mycobacterium and Its Application to Engineer a Vitamin B12 Whole-Cell Ribosensor 分枝杆菌蛋氨酸代谢的进化轨迹及其在构建维生素B12全细胞核糖传感器中的应用

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-08 DOI: 10.1111/1751-7915.70176

Elena Campos-Pardos, Laura Sanz-Asensio, Sandra Pérez-Jiménez, Inmaculada Yruela, Bruno Contreras-Moreira, Alejandro Toledo-Arana, Jesús Gonzalo-Asensio

{"title":"Evolutionary Trajectories of Methionine Metabolism in Mycobacterium and Its Application to Engineer a Vitamin B12 Whole-Cell Ribosensor","authors":"Elena Campos-Pardos, Laura Sanz-Asensio, Sandra Pérez-Jiménez, Inmaculada Yruela, Bruno Contreras-Moreira, Alejandro Toledo-Arana, Jesús Gonzalo-Asensio","doi":"10.1111/1751-7915.70176","DOIUrl":"https://doi.org/10.1111/1751-7915.70176","url":null,"abstract":"Vitamin B12 metabolism differs among members of the Mycobacterium genus. While non-tuberculous mycobacterial species are B12 producers, tuberculous mycobacteria lack endogenous production and rely on the host supply of this vitamin. Here, we hypothesise that this discrepant phenotype might impact the function of B12-dependent enzymes. We specifically focused on methionine synthases MetH and MetE. Both enzymes showed genetic differences in the Mycobacterium genus, resulting in a clear divergence between tuberculous and non-tuberculous species. Unexpectedly, the dependency of MetH on B12 was indistinguishable between M. tuberculosis and M. smegmatis, assayed as representative members of tuberculous and non-tuberculous species, respectively. However, MetE showed robust phenotypic differences between these species, displaying a finely tuned B12 regulation in M. tuberculosis, in contrast to a more permissive regulation in M. smegmatis. Both orthologs differ in the vitamin isoform specifically recognised, and the B12 threshold level required for MetE regulation. Since the B12 regulatory element in the metE gene is an RNA riboswitch, we analysed the polymorphisms in this region, with a special focus on loss-of-function mutations identified after in vitro selection. We used this information to engineer a whole-cell B12 biosensor in the genetically fastidious Mycobacterium genus, being able to detect vitamin B12 concentration in the range of micrograms per millilitre.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70176","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chicken Manure as a Sustainable Bile Acid Source for Biotechnological Applications 鸡粪作为生物技术应用的可持续胆汁酸来源

IF 5.7 2区生物学

Microbial Biotechnology Pub Date : 2025-06-08 DOI: 10.1111/1751-7915.70178

Johannes Holert, Rudolf Wilhelm, Jens Henker, Claudia A. Reinker, Franziska M. Müller, Bodo Philipp

{"title":"Chicken Manure as a Sustainable Bile Acid Source for Biotechnological Applications","authors":"Johannes Holert, Rudolf Wilhelm, Jens Henker, Claudia A. Reinker, Franziska M. Müller, Bodo Philipp","doi":"10.1111/1751-7915.70178","DOIUrl":"https://doi.org/10.1111/1751-7915.70178","url":null,"abstract":"Ursodeoxycholic acid (UDCA) is widely administered to dissolve gallstones, treat liver disorders and reduce blood cholesterol levels. This study investigated fresh and dried chicken manure as a sustainable bioresource for chenodeoxycholic acid (CDCA), a precursor for the biotechnological production of UDCA. For this, bile acids from five commercial dried and seven fresh chicken manure samples were analysed. The bile acid pool consisted predominantly of CDCA (30%–90%) and 7-keto lithocholic acid (7k-LCA, 8%–56%), with minor amounts of cholic acid. CDCA concentrations varied between 62 and 2990 mg per kg dry weight, and the highest concentrations were found in two samples from fresh chicken manure, confirming that chickens can produce high but varying amounts of faecal CDCA. As a proof of principle, a newly created Pseudomonas putida KT2440 strain expressing a heterologous 7α−/7β-hydroxysteroid dehydrogenase system was shown to be able to transform manure-derived CDCA into UDCA without prior substrate purification from raw ethanolic chicken manure extracts. These results demonstrate that chicken manure can be used as an untapped resource for bile acids for biotechnological applications, providing a novel approach for the valorisation of this bioresource.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 6","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0