Microbial Biotechnology最新文献_第3页

One-Pot RPA-CRISPR/Cas12a Assay With Visual Readout for the Ultra-Specific Detection of Monkeypox Virus Clade I 单罐RPA-CRISPR/Cas12a可视化检测猴痘病毒进化支I的方法

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-09-06 DOI: 10.1111/1751-7915.70437

Boyi Li, Luyao Liu, Kaikai Jin, Zanheng Huang, Tianyi Zhang, Rong Gao, Huanxin Chen, Lijuan Niu, Changqi Fan, Haili Zhang, Pei Huang, Hualei Wang

{"title":"One-Pot RPA-CRISPR/Cas12a Assay With Visual Readout for the Ultra-Specific Detection of Monkeypox Virus Clade I","authors":"Boyi Li, Luyao Liu, Kaikai Jin, Zanheng Huang, Tianyi Zhang, Rong Gao, Huanxin Chen, Lijuan Niu, Changqi Fan, Haili Zhang, Pei Huang, Hualei Wang","doi":"10.1111/1751-7915.70437","DOIUrl":"10.1111/1751-7915.70437","url":null,"abstract":"In 2024, Monkeypox virus (MPXV) clade I has triggered outbreaks in several countries world-wide. MPXV clade I demonstrates enhanced virulence and transmissibility, with a case fatality rate reaching 10%. In response, we have developed a one-pot detection assay specifically targeting MPXV clade I, combining recombinase polymerase amplification (RPA) and the CRISPR/Cas12a system. The assay can be completed within 40 min and achieved a 95% limit of detection (LOD95) of 27.16 copies/μL. No cross-reactivity was observed with MPXV clade II or other tested viral templates, including Vaccinia virus (Tiantan strain). A preliminary room-temperature evaluation showed that the assay retained detectable performance at 25°C, supporting its potential use in equipment-limited settings. The assay also showed good intra-assay and inter-assay repeatability for recombinant plasmid templates, with all coefficient of variation (CV) values below 10%. In simulated clinical samples, the RPA-CRISPR/Cas12a assay detected more low-concentration plasmid-spiked samples than quantitative polymerase chain reaction (qPCR). These results indicate that the established assay is specific, sensitive, repeatable, and easy to perform, providing a practical tool for field-based screening and decentralized detection of MPXV clade I.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 9","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13547839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Lytic Bacteriophage Cocktail Attenuates Carbapenem-Resistant Klebsiella pneumoniae–Acinetobacter baumannii Mixed-Species Biofilms and Reshapes Transcriptional Responses 溶解噬菌体鸡尾酒减弱耐碳青霉烯肺炎克雷伯菌-鲍曼不动杆菌混合物种生物膜并重塑转录反应。

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-09-03 DOI: 10.1111/1751-7915.70439

Xuefang Ren, Rui Zhu, Yijiang Yang, Xiaojuan You, Liuxiang Wang, Guangyu Fu, Yehuan Zheng, Chunxia Wang, Zhentao Wang, Yongwei Li

{"title":"A Lytic Bacteriophage Cocktail Attenuates Carbapenem-Resistant Klebsiella pneumoniae–Acinetobacter baumannii Mixed-Species Biofilms and Reshapes Transcriptional Responses","authors":"Xuefang Ren, Rui Zhu, Yijiang Yang, Xiaojuan You, Liuxiang Wang, Guangyu Fu, Yehuan Zheng, Chunxia Wang, Zhentao Wang, Yongwei Li","doi":"10.1111/1751-7915.70439","DOIUrl":"10.1111/1751-7915.70439","url":null,"abstract":"Mixed-species biofilms formed by carbapenem-resistant Klebsiella pneumoniae and carbapenem-resistant Acinetobacter baumannii can complicate the clinical management of device-associated infections; however, phage-based interventions targeting these communities remain insufficiently characterized. We assessed the antibiofilm activity of a lytic HZJ31 + HZY2308 phage cocktail against KPZ2–AB48 mixed-species biofilms. Compared with the corresponding single-species cultures, cocultures showed greater crystal violet-stained biomass, higher metabolic activity as measured by the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay, and microscopic surface coverage. Phage-cocktail exposure during biofilm development or after biofilm formation reduced biomass, XTT reduction, culturable bacterial counts, and scanning electron microscopy-derived surface coverage, with similar changes observed on central venous catheter surfaces. In Galleria mellonella infection models, cocktail treatment increased survival and was associated with less extensive histopathological lesions and lower Kirschner wire-associated bacterial burdens. RNA sequencing and quantitative reverse transcription polymerase chain reaction identified treatment-associated transcriptional changes involving metabolic pathways, autoinducer-2 transport, cell-envelope functions, efflux systems, and stress responses. These findings support further preclinical investigation of phage cocktails targeting multidrug-resistant mixed-species biofilms.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 9","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13542435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148885948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of a Bacteriophage-Based Product for Biocontrol of Fire Blight and Black Rot 基于噬菌体的炭黑病生物防治产品的设计。

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-09-02 DOI: 10.1111/1751-7915.70436

Gloria Vique, Pedro Blanco-Picazo, Aina Trenchs, Maria Dolores Ramos-Barbero, Maria Isern, Pablo Quirós, Sergio Atares, Ignasi Salaet, Laura Sala-Comorera, Lorena Rodríguez-Rubio, Maite Muniesa

{"title":"Design of a Bacteriophage-Based Product for Biocontrol of Fire Blight and Black Rot","authors":"Gloria Vique, Pedro Blanco-Picazo, Aina Trenchs, Maria Dolores Ramos-Barbero, Maria Isern, Pablo Quirós, Sergio Atares, Ignasi Salaet, Laura Sala-Comorera, Lorena Rodríguez-Rubio, Maite Muniesa","doi":"10.1111/1751-7915.70436","DOIUrl":"10.1111/1751-7915.70436","url":null,"abstract":"Controlling phytopathogenic bacteria is essential for safeguarding crops and minimizing economic losses in agriculture. Although chemical pesticides are effective, they have adverse effects on ecosystems, beneficial organisms such as pollinators, and natural pathogen predators, creating a need for sustainable alternatives. Bacteriophages ɸEF1 and ɸEF2 (infecting Erwinia amylovora, the fire blight pathogen), and ɸXF1 (targeting Xanthomonas campestris, the black rot pathogen) have shown promise as biocontrol agents in vitro and in vivo. However, to be effective in the field these phages must maintain infectivity during storage and environmental exposure. We evaluated their stability under varying pH, temperature, UV and sunlight conditions and assessed various photoprotective additives. The phages remained infectious for up to 3 months at 4°C and 20°C and pH 7–9, but were inactivated at pH 3 and 37°C. UV rapidly inactivated the phages, but their stability was significantly enhanced by adding photoprotectants like Amino 22% or CaCO3, particularly CaCO3 at 24%, which preserved infectivity for over 8 h. These photoprotectants also improved phage recovery from plant surfaces. We conclude that effective biocontrol formulations should contain ≥ 106 pfu/mL, maintain neutral pH, include 24% CaCO3, and allow room temperature storage. This study is among the first to demonstrate the long-term stability of phage-based formulations at room temperature, together with improved field persistence, supporting their potential use as biopesticides.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 9","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13535983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rewiring Carbon Metabolism in Bacillus methanolicus via Heterologous Phosphoketolase Expression Enhances Biomass Yield From Methanol and Reduces CO2 Loss 通过异源磷酸酮醇酶表达重组甲醇芽孢杆菌的碳代谢提高甲醇生物质产量并减少CO2损失

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-09-01 DOI: 10.1111/1751-7915.70430

Yael J. Toporek, Jonathan R. Humphreys, Thomas J. Musselwhite, Marcus S. Bray, Katie P. Michel, Kimberly A. Rosenbach, Michael T. Guarnieri

{"title":"Rewiring Carbon Metabolism in Bacillus methanolicus via Heterologous Phosphoketolase Expression Enhances Biomass Yield From Methanol and Reduces CO2 Loss","authors":"Yael J. Toporek, Jonathan R. Humphreys, Thomas J. Musselwhite, Marcus S. Bray, Katie P. Michel, Kimberly A. Rosenbach, Michael T. Guarnieri","doi":"10.1111/1751-7915.70430","DOIUrl":"https://doi.org/10.1111/1751-7915.70430","url":null,"abstract":"Methylotrophic microbes are attractive alternatives to traditional heterotrophic production platforms, yet their efficiency is constrained by carbon loss through pyruvate decarboxylation and the oxidative branch of the RuMP cycle. The phosphoketolase (PKT) pathway provides a carbon-conserving alternative by cleaving fructose-6-phosphate and/or xylulose-5-phosphate into acetyl-phosphate, which can subsequently be converted to acetyl-coA without pyruvate decarboxylation. The remaining carbon intermediates are recycled through central metabolism to regenerate RuMP cycle intermediates without direct CO2 release. Here, we engineered this strategy in Bacillus methanolicus, a thermophilic methylotroph with strong industrial potential. We first established a versatile expression toolkit comprising inducible and constitutive promoters, benchmarked using an sfGFP reporter. Leveraging this system, we heterologously expressed the phosphoketolase B (pktB) gene from Methylotuvimicrobium buryatense 5GB1C which increased methanol-to-biomass yields by 18%–24% relative to controls and reduced biogenic CO2 production by 9%–12%. Chromosomal integration of pktB preserved these gains, demonstrating stability without reliance on plasmid-based expression. Together, these results show that PKT-driven metabolic rewiring enhances substrate yields in B. methanolicus and provides a scalable strategy to improve methylotrophic bioprocesses. This work expands the metabolic engineering toolbox for methylotrophs and highlights carbon-conserving pathway design as a key lever for advancing single carbon (C1) biomanufacturing.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 9","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70430","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rewiring Carbon Metabolism in Bacillus methanolicus via Heterologous Phosphoketolase Expression Enhances Biomass Yield From Methanol and Reduces CO2 Loss 通过异源磷酸酮醇酶表达重组甲醇芽孢杆菌的碳代谢提高甲醇生物质产量并减少CO2损失

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-09-01 DOI: 10.1111/1751-7915.70430

Yael J. Toporek, Jonathan R. Humphreys, Thomas J. Musselwhite, Marcus S. Bray, Katie P. Michel, Kimberly A. Rosenbach, Michael T. Guarnieri

{"title":"Rewiring Carbon Metabolism in Bacillus methanolicus via Heterologous Phosphoketolase Expression Enhances Biomass Yield From Methanol and Reduces CO2 Loss","authors":"Yael J. Toporek, Jonathan R. Humphreys, Thomas J. Musselwhite, Marcus S. Bray, Katie P. Michel, Kimberly A. Rosenbach, Michael T. Guarnieri","doi":"10.1111/1751-7915.70430","DOIUrl":"https://doi.org/10.1111/1751-7915.70430","url":null,"abstract":"Methylotrophic microbes are attractive alternatives to traditional heterotrophic production platforms, yet their efficiency is constrained by carbon loss through pyruvate decarboxylation and the oxidative branch of the RuMP cycle. The phosphoketolase (PKT) pathway provides a carbon-conserving alternative by cleaving fructose-6-phosphate and/or xylulose-5-phosphate into acetyl-phosphate, which can subsequently be converted to acetyl-coA without pyruvate decarboxylation. The remaining carbon intermediates are recycled through central metabolism to regenerate RuMP cycle intermediates without direct CO2 release. Here, we engineered this strategy in Bacillus methanolicus, a thermophilic methylotroph with strong industrial potential. We first established a versatile expression toolkit comprising inducible and constitutive promoters, benchmarked using an sfGFP reporter. Leveraging this system, we heterologously expressed the phosphoketolase B (pktB) gene from Methylotuvimicrobium buryatense 5GB1C which increased methanol-to-biomass yields by 18%–24% relative to controls and reduced biogenic CO2 production by 9%–12%. Chromosomal integration of pktB preserved these gains, demonstrating stability without reliance on plasmid-based expression. Together, these results show that PKT-driven metabolic rewiring enhances substrate yields in B. methanolicus and provides a scalable strategy to improve methylotrophic bioprocesses. This work expands the metabolic engineering toolbox for methylotrophs and highlights carbon-conserving pathway design as a key lever for advancing single carbon (C1) biomanufacturing.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 9","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70430","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-Clinical Development of Two DNA Vaccines Encoding MrkA of Klebsiella pneumoniae: Effect of Plasmid Backbone on Immunogenicity and Protective Efficacy Against Sepsis 两种编码肺炎克雷伯菌MrkA的DNA疫苗的临床前研制：质粒主干对脓毒症免疫原性和保护作用的影响

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-08-23 DOI: 10.1111/1751-7915.70434

Marta Vicente-Pazos, Estéfani García-Ríos, Laura Quílez-Agraz, Gal·la Ustrell-Sallent, Pilar Pérez-Romero, Mireia López-Siles, Michael J. McConnell

{"title":"Pre-Clinical Development of Two DNA Vaccines Encoding MrkA of Klebsiella pneumoniae: Effect of Plasmid Backbone on Immunogenicity and Protective Efficacy Against Sepsis","authors":"Marta Vicente-Pazos, Estéfani García-Ríos, Laura Quílez-Agraz, Gal·la Ustrell-Sallent, Pilar Pérez-Romero, Mireia López-Siles, Michael J. McConnell","doi":"10.1111/1751-7915.70434","DOIUrl":"10.1111/1751-7915.70434","url":null,"abstract":"Development of vaccines against Klebsiella pneumoniae has potential to prevent infections in both community and hospital settings and reduce associated mortality and morbidity worldwide. DNA vaccines represent a promising approach given their stability, safety, design flexibility and cost-effectiveness for rapid production. In this study, two plasmid backbones, namely the commercial pVAX1 and the newly designed PLASMIVAX (containing additional CpG motifs), were tested to develop two DNA vaccines targeting the MrkA fimbrial subunit from K. pneumoniae. MrkA is highly conserved among K. pneumoniae and was expressed and secreted in vitro, fused to immunostimulatory elements, by both DNA vaccines following transfection of HEK-293T cells. Immunogenicity was assessed in mice by measuring antibody levels (IgM, IgG, IgG1, IgG2a and IgG3) after intramuscular immunization with three doses of 50 μg of each DNA vaccine. Efficacy of protection was established in a sepsis infection model. The pVAX1-MrkA DNA vaccine elicited the most robust antibody response and protection against K. pneumoniae infection (80%, p = 0.0373), while the PLASMIVAX-MrkA DNA vaccine protected at a lower rate (30%, p = 0.0163), consistent with lower post-immunization antibody titers elicited by this vaccine. This study reveals that DNA plasmid properties strongly influence immunogenicity and vaccine efficacy and highlights the potential role of MrkA as a vaccine antigen and DNA platforms as a valuable tool for developing vaccines against multidrug-resistant bacteria.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 8","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-Guided Extremophile Genome Mining Expands the PETase Landscape and Reveals PET-Hydrolysing True Lipase Lineages 结构导向的极端微生物基因组挖掘扩展了pet酶景观并揭示了pet水解的真正脂肪酶谱系

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-08-21 DOI: 10.1111/1751-7915.70432

Rubén Muñoz-Tafalla, José M. González-Romero, Paula Vidal, Laura Fernandez-Lopez, Ana Robles-Martín, David Almendral, José L. Gonzalez-Alfonso, Francisco J. Plou, Manuel Ferrer, Víctor Guallar, Rafael Bargiela

{"title":"Structure-Guided Extremophile Genome Mining Expands the PETase Landscape and Reveals PET-Hydrolysing True Lipase Lineages","authors":"Rubén Muñoz-Tafalla, José M. González-Romero, Paula Vidal, Laura Fernandez-Lopez, Ana Robles-Martín, David Almendral, José L. Gonzalez-Alfonso, Francisco J. Plou, Manuel Ferrer, Víctor Guallar, Rafael Bargiela","doi":"10.1111/1751-7915.70432","DOIUrl":"https://doi.org/10.1111/1751-7915.70432","url":null,"abstract":"Poly(ethylene terephthalate) (PET) hydrolases are enzymes primarily within the polyesterase–cutinase branch of the α/β-hydrolase superfamily, whereas the contribution of true lipases to PET hydrolysis remains poorly explored. Here, we report the genome mining results of 18,082 extremophilic microorganisms, combined with structural modelling, enzyme–substrate simulations and experimental validation, which enables the identification of PET-active true lipases. Two true lipases, LipBv and LipSh1, hydrolyse PET substrates and homologous enzymes within their respective clusters also retain PET-hydrolysing activity, supporting the existence of specific lipase lineages associated with PET hydrolysis. Structural analyses suggest that differences in loop organization and active-site accessibility may contribute to the observed PET-hydrolysing activity and hydrolysis product profiles. These lipase lineages clustered separately from 1322 putative PETase homologues from the same extremophile genomes, while all groups remained distinct from previously characterized PETases. These findings expand the evolutionary diversity of PET-hydrolysing enzymes in extremophiles.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 8","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Safe and Effective Bivalent Vaccine Candidate Against Duck Enteritis Virus and Duck-Origin Novel Goose Parvovirus 一种安全有效的鸭肠炎病毒和鸭源新型鹅细小病毒二价候选疫苗

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-08-19 DOI: 10.1111/1751-7915.70433

Liu Chen, Jionggang Hua, Weicheng Ye, Cun Zhang, Xinjin Li, Yinchu Zhu, Yuan Fu, Liang He, Suxin Huo, Zongxiao Wang, Zhuanli Wu, Tao Yun

{"title":"A Safe and Effective Bivalent Vaccine Candidate Against Duck Enteritis Virus and Duck-Origin Novel Goose Parvovirus","authors":"Liu Chen, Jionggang Hua, Weicheng Ye, Cun Zhang, Xinjin Li, Yinchu Zhu, Yuan Fu, Liang He, Suxin Huo, Zongxiao Wang, Zhuanli Wu, Tao Yun","doi":"10.1111/1751-7915.70433","DOIUrl":"https://doi.org/10.1111/1751-7915.70433","url":null,"abstract":"Since 2015, duck-origin novel goose parvovirus (NGPV) has caused severe economic losses to the Chinese duck industry, with no licensed vaccines or treatments available. Our previous study constructed a recombinant duck enteritis virus (rDEV) from an attenuated vaccine strain, which conferred full protection against virulent DEV challenge. NGPV VP2 is the major immunogenic structural antigen. In this study, we generated a recombinant virus rNGPV VP2-Cre by inserting the NGPV VP2 gene into the rDEV genome. VP2 expression and virus-like particle assembly were confirmed. Safety and efficacy were evaluated in ducklings. The results showed that rNGPV VP2-Cre was safe, facilitated clearance of NGPV and provided strong protection against challenge with a highly virulent DEV strain. In summary, rNGPV VP2-Cre represents a promising bivalent vaccine candidate for concurrent control of DEV and NGPV infections.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 8","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of Length and Hydrophobicity Requirements for Optimal α-Amylase Secretion in Bacillus subtilis Based on Rational Design 基于合理设计的枯草芽孢杆菌α-淀粉酶最佳分泌长度和疏水性要求的探索

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-08-17 DOI: 10.1111/1751-7915.70435

Dicky A. Pranoto, Danilo Milo, Lorenzo de la Parra Soto, Jolanda Neef, Girbe Buist, Jan Maarten van Dijl

{"title":"Exploration of Length and Hydrophobicity Requirements for Optimal α-Amylase Secretion in Bacillus subtilis Based on Rational Design","authors":"Dicky A. Pranoto, Danilo Milo, Lorenzo de la Parra Soto, Jolanda Neef, Girbe Buist, Jan Maarten van Dijl","doi":"10.1111/1751-7915.70435","DOIUrl":"10.1111/1751-7915.70435","url":null,"abstract":"The secretory (Sec) pathway is the major pathway of Bacillus subtilis for protein export from the cytoplasm to the extracellular milieu. An amino-terminal signal peptide is required to initiate protein translocation across the cytoplasmic membrane via Sec. Generally, Sec pathway signal peptides of B. subtilis are around 20–30 amino acids long and tend to be relatively hydrophobic compared to signal peptides from other organisms. In view of the importance of signal peptides for high-level secretory protein production, understanding how signal peptide structure and length influence the efficiency of protein secretion is crucial. Accordingly, the present study was aimed at investigating the effects of signal peptide size and, in particular the subdomain sizes of a signal peptide, in relation to protein secretion efficiency. To this end, a rational engineering approach was employed for the design of shortened signal peptides that can still drive effective protein secretion. This involved in silico interaction studies with the signal recognition particle, which is involved in protein targeting from the ribosome to the membrane. Our results show that the hydrophobic H-domain of a B. subtilis signal peptide can be shortened from seventeen to seven hydrophobic amino acid residues without a detectable decrease in protein secretion.","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"19 8","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70435","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148756968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility and Safety of Washed Microbiota Transplantation as Salvage Therapy for Refractory Non-CDI Gut Pathogens in Critically Ill Patients 洗涤菌群移植作为危重患者难治性非cdi肠道病原菌抢救治疗的可行性和安全性

IF 6.7 2区生物学

Microbial Biotechnology Pub Date : 2026-08-13 DOI: 10.1111/1751-7915.70431

Jie Xu, Aoli Diao, Rujun Ai, Xia Wu, Quan Wen, Yuyan Xiao, Xinyi He, You Yu, Zulun Zhang, Yeqing Wang, Zhihua Zhang, Faming Zhang, Bota Cui

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