Molecular Strategies to Overcome Fungal Virulence in Crop Protection

Fungal pathogens are major threats to global crop production, intensified by rising fungicide resistance and the limited availability of resistant cultivars. This highlight article outlines recent molecular strategies aimed at reducing fungal virulence through sustainable and targeted approaches. RNA interference (RNAi) has emerged as a precise method to silence essential genes in pathogens, significantly impairing virulence and development. In parallel, inhibiting fungal efflux transporters—particularly ABC and MFS proteins—has been shown to reverse multidrug resistance and restore fungicide efficacy in pathogens like Botrytis cinerea. Additionally, engineering biocontrol agents expressing anti-apoptotic genes enhances their growth, stress resistance, and mycoparasitic activity. These strategies collectively illustrate the potential of combining RNAi technologies, efflux inhibition, and genetically enhanced biocontrol agents to create integrated, environmentally friendly plant protection systems. Such precision-targeted approaches represent a promising alternative to traditional chemical control, aligning with global efforts to achieve sustainable agriculture.