Biomechanics and Modeling in Mechanobiology最新文献

Investigation of particle penetration in a nasal maxillary ostium with optimised T-junction geometry and pulsatile flow. 利用优化的t型结几何形状和脉冲流研究颗粒在鼻上颌口的渗透。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-10-06 DOI: 10.1007/s10237-025-02015-9

Patrick Warfield-McAlpine, David F Fletcher, Kiao Inthavong

{"title":"Investigation of particle penetration in a nasal maxillary ostium with optimised T-junction geometry and pulsatile flow.","authors":"Patrick Warfield-McAlpine, David F Fletcher, Kiao Inthavong","doi":"10.1007/s10237-025-02015-9","DOIUrl":"https://doi.org/10.1007/s10237-025-02015-9","url":null,"abstract":"Effective drug delivery to the maxillary sinus is often limited by the narrow and variable shape of the maxillary ostium. To better understand and predict how surgical changes affect drug transport, the ostium can be modelled as a simplified T-junction. The geometric configuration of these junctions plays a crucial role in managing particle flow; however, optimal design parameters remain under-explored. This paper addresses this gap, by simulating a range of radius of curvatures <math><msub><mi>R</mi> <mtext>c</mtext></msub> </math> at the T-junction and oscillatory flows with pulsation frequencies of 0, 30, 45, 60 and 75 Hz to analyse their effects on particle penetration and distribution. The results revealed that an anterior <math><msub><mi>R</mi> <mtext>c</mtext></msub> </math> enhanced particle outflow through the y-branch (perpendicular) outlet, while a posterior <math><msub><mi>R</mi> <mtext>c</mtext></msub> </math> limited this outflow. Comparisons of pulsating frequencies further showed that a lower frequency improved penetration into the y-branch. Interestingly, applying both anterior and posterior <math><msub><mi>R</mi> <mtext>c</mtext></msub> </math> did not yield better performance than an anterior <math><msub><mi>R</mi> <mtext>c</mtext></msub> </math> alone. Furthermore, a constant flow rate where <math><mrow><mi>f</mi> <mo>=</mo> <mn>0</mn></mrow> </math> Hz promoted greater particle outflow through the y-branch in the T-junction model. However, a pulsating frequency of 30 Hz improved deposition in the nasal airway. The study underscores the potential of targeted geometric adjustments to optimise flow and deposition in the maxillary ostium, providing valuable insight into drug delivery strategies and inhalation toxicology.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An MRI-derived head-neck finite element model. 核磁共振成像头颈有限元模型。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-10-03 DOI: 10.1007/s10237-025-02013-x

Hossein Bahreinizad, Gustavo M Paulon, Leonardo Wei, Suman K Chowdhury

{"title":"An MRI-derived head-neck finite element model.","authors":"Hossein Bahreinizad, Gustavo M Paulon, Leonardo Wei, Suman K Chowdhury","doi":"10.1007/s10237-025-02013-x","DOIUrl":"https://doi.org/10.1007/s10237-025-02013-x","url":null,"abstract":"This study aimed to develop and validate a magnetic resonance imaging (MRI)-derived biofidelic head-neck finite element (FE) model comprised of scalp, skull, CSF, brain, dura mater, pia mater, cervical vertebrae, and disks, 14 ligaments, and 42 neck muscles. We developed this model using head and neck MRI images of a healthy male participant and by implementing a novel brain hexahedral meshing algorithm and a scalp erosion model. The model was validated by replicating three experimental studies: Alshareef's brain sonomicrometry study, NBDL's high-acceleration profile, and Ito's frontal impact cervical vertebrae study. The results also showed that the segmented geometries of the model aligned closely with the literature data (within 3 <math><mi>σ</mi></math> limit). The brain displacement results of the model aligned well (r = 0.48-0.96) with those reported in Alshareef's experimental study. The head-neck kinematic responses of the model showed a strong correlation (r > 0.97) with the NBDL's experimental results. The simulation of Ito's experimental condition yielded peak shear strain values of the cervical spine within 1 <math><mi>σ</mi></math> of the experimental data. Our developed head-neck FE model provides an effective computational platform for advancing brain and head injury biomechanics research and evaluating protective equipment in various impact scenarios.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and numerical analysis of a prosthetic mitral valve with a lantern-shaped variable diameter stent for TMVR. 灯笼状变径支架用于TMVR的人工二尖瓣设计与数值分析。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-09-24 DOI: 10.1007/s10237-025-02008-8

Xuechao Ding, Shiliang Chen, Tianming Du, Yanping Zhang, Shengwen Liu, Aike Qiao

{"title":"Design and numerical analysis of a prosthetic mitral valve with a lantern-shaped variable diameter stent for TMVR.","authors":"Xuechao Ding, Shiliang Chen, Tianming Du, Yanping Zhang, Shengwen Liu, Aike Qiao","doi":"10.1007/s10237-025-02008-8","DOIUrl":"https://doi.org/10.1007/s10237-025-02008-8","url":null,"abstract":"Transcatheter mitral valve replacement (TMVR) faces challenges of stent migration and left ventricular outflow tract (LVOT) obstruction. Traditional stents fail to meet the demands of systolic high pressure, dynamic saddle-shaped annular contraction, and diastolic LVOT protection, while auxiliary anchoring devices may cause tissue damage. To address these issues, we propose a dual-layer lantern-shaped nitinol stent (L-NiTi) with a pressure-responsive diameter modulation. Using SAPIEN 3 Ultra cylindrical cobalt-chromium (C-CoCr) and cylindrical nitinol (C-NiTi) stents as controls, we constructed a finite element native valve stent prosthesis interaction model under cardiac cycle pressure loading to quantify the performance of the stents. Results showed that the L-NiTi exhibited a maximum strain of 8.9%, a 9.17% ± 3.12% loss in prosthetic leaflet area (compared to a 23% loss in controls), a 34 N increase in systolic migration resistance, and an axial displacement of 1.28 mm (compared to 2.16 and 4.78 mm in C-CoCr and C-NiTi controls, respectively). The improved asymmetric lantern-shaped stent maintained a 32 N increase in migration resistance while increasing the neo-LVOT area from 2.52 to 2.81 cm2. The proposed new design of stent for TMVR enhances anchoring without compromising LVOT, demonstrating translational potential for TMVR.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical stress profiling in coronary arteries via two-phase blood FSI. 冠状动脉两期血流FSI生物力学应力谱分析。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-09-22 DOI: 10.1007/s10237-025-02012-y

Farajollah Zare Jouneghani, Reza Ghomashchi, Marco Amabili, Mergen H Ghayesh

{"title":"Biomechanical stress profiling in coronary arteries via two-phase blood FSI.","authors":"Farajollah Zare Jouneghani, Reza Ghomashchi, Marco Amabili, Mergen H Ghayesh","doi":"10.1007/s10237-025-02012-y","DOIUrl":"https://doi.org/10.1007/s10237-025-02012-y","url":null,"abstract":"This study focuses on the biomechanical stress determination of the left circumflex (LCx) coronary artery reconstructed based on in vivo angiography images via the development of a comprehensive biomechanical model incorporating a two-phase two-way coupled three-dimensional fluid-structure interaction (FSI). The blood flow is modelled as a two-phase pulsatile fluid, with 45% red blood cells and 55% plasma, and the artery wall is modelled as a soft viscohyperelastic material that is able to dynamically react to the blood-induced pressure. The flow characteristics, such as pressure, velocity, phase distribution, near-wall haemodynamic parameters, and flow-induced indices, are determined. The von Mises stress (VMS) and the deformation field of the arterial wall are also obtained. Comparing results based on the two-phase FSI model and those of a single-phase FSI show that taking into account the red blood cells alters the stresses, providing a better understanding of potential cardiovascular events. In all the cases investigated in this study, the wall shear stress (WSS) levels predicted by the two-phase FSI model are consistently lower than those obtained from the single-phase simulations. For example, at the location of maximum WSS during peak systole, the single-phase simulation employing the Quemada viscosity model predicts 143.43 Pa, whereas the single-phase simulation based on the power-law model predicts 39.85 Pa. In contrast, the two-phase model yields a substantially lower value of 24.79 Pa.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145123796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward a novel stent retriever: design, optimization and experimental validation. 一种新型支架回收器：设计、优化和实验验证。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-09-19 DOI: 10.1007/s10237-025-01993-0

Shuo Wu, Francesca Berti, Xianming Wang, Bo Wang, Yimin Luo, Tianxiao Zhang, Shengnan Lyu

{"title":"Toward a novel stent retriever: design, optimization and experimental validation.","authors":"Shuo Wu, Francesca Berti, Xianming Wang, Bo Wang, Yimin Luo, Tianxiao Zhang, Shengnan Lyu","doi":"10.1007/s10237-025-01993-0","DOIUrl":"https://doi.org/10.1007/s10237-025-01993-0","url":null,"abstract":"Acute ischemic stroke remains a leading cause of global disability and mortality. While mechanical thrombectomy with stent retrievers has improved outcomes through rapid reperfusion, the main limitation is the lack of conformability in tortuous and bifurcated arteries, thus reducing the thrombus retention efficacy in such complex vascular anatomies. This study introduces a novel self-expandable stent retriever design featuring a segmented closed-cell structure with bridging elements, designed to enhance both radial force and flexibility. Finite element analysis evaluated mechanical performance under different loading configurations, aiming at assessing a few key biomechanical parameters such as maximum principal strain and radial force. Then, a multi-objective optimization was performed to increase the device radial force while maintaining low strains. Compared to commercial devices, the optimized stent demonstrated a 18.2% lower bending moment and maintained cross-sectional geometry more effectively under deformation, indicating improved flexibility and shape preservation during navigation in tortuous vessels. Preliminary proof-of-concept in vitro thrombectomy experiments demonstrated effective engagement with mechanically stiff thrombi in different realistic scenarios, such as in stenotic and curved vessel models. While retrieval in bifurcated models still presents some challenges, the results suggest that the proposed design offers a promising balance between flexibility and radial strength, potentially improving thrombectomy outcomes in complex vascular environments.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A hybrid reduced order model to enforce outflow pressure boundary conditions in computational hemodynamics. 计算血流动力学中输出压力边界条件的混合降阶模型。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-09-15 DOI: 10.1007/s10237-025-02007-9

Pierfrancesco Siena, Pasquale Claudio Africa, Michele Girfoglio, Gianluigi Rozza

{"title":"A hybrid reduced order model to enforce outflow pressure boundary conditions in computational hemodynamics.","authors":"Pierfrancesco Siena, Pasquale Claudio Africa, Michele Girfoglio, Gianluigi Rozza","doi":"10.1007/s10237-025-02007-9","DOIUrl":"https://doi.org/10.1007/s10237-025-02007-9","url":null,"abstract":"This paper deals with the development of a reduced order model (ROM) which could be used as an efficient tool for the reconstruction of the unsteady blood flow patterns in cardiovascular applications. The methodology relies on proper orthogonal decomposition to compute basis functions, combined with a Galerkin projection to compute the reduced coefficients. The main novelty of this work lies in the extension of the lifting function method, which typically is adopted for treating nonhomogeneous inlet velocity boundary conditions, to the handling of nonhomogeneous outlet boundary conditions for pressure, representing a very delicate point in numerical simulations of cardiovascular systems. Moreover, we incorporate a properly trained neural network in the ROM framework to approximate the mapping from time parameter to outflow pressure, which in the most general case is not available in closed form. We define our approach as \"hybrid\", because it merges equation-based elements with purely data-driven ones. The full order model (FOM) is related to a finite volume method which is employed for the discretization of unsteady Navier-Stokes equations while a two-element Windkessel model is adopted to enforce a reliable estimation of outflow pressure. Numerical results, firstly related to a 3D idealized blood vessel and then to a 3D patient-specific aortic arch, demonstrate that our ROM is able to accurately approximate the FOM with a significant reduction in computational cost.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Midgut protrusion, rotation, and retraction induced by temporal alteration in differential growth. 中肠的突出，旋转和收缩引起的时间改变的差异生长。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-09-13 DOI: 10.1007/s10237-025-01999-8

Michina Saiki, Hironori Takeda, Yuto Kawabata, Shunichi Ishida, Yohsuke Imai

{"title":"Midgut protrusion, rotation, and retraction induced by temporal alteration in differential growth.","authors":"Michina Saiki, Hironori Takeda, Yuto Kawabata, Shunichi Ishida, Yohsuke Imai","doi":"10.1007/s10237-025-01999-8","DOIUrl":"https://doi.org/10.1007/s10237-025-01999-8","url":null,"abstract":"Herniation, rotation, looping, and retraction of the midgut occur sequentially during midgut morphogenesis. Recent studies have demonstrated the importance of mechanical forces arising from the differential growth between the midgut and mesentery in the formation of small intestinal loops. However, the roles of mechanics and differential growth in the overall process remain unclear. In this study, we developed a computational model of midgut morphogenesis based on continuum mechanics. We showed that the protrusion, rotation, and retraction of the midgut can emerge sequentially because of temporal changes in differential growth. The midgut was modeled as a hyperelastic tube with a Gaussian shape. The differential growth of the midgut and mesentery was modeled by the spatial variation in spontaneous plastic deformation. The hyperelastic tube developed a protrusion by compression-induced deformation, suggesting that other external forces are not necessary for midgut herniation prior to rotation. Appropriate differential growth induced a <math><msup><mn>90</mn> <mo>∘</mo></msup> </math> rotation of the tube. A less-growing mesentery attempts to face inward to minimize the tensile forces, which causes tube twisting and results in midgut rotation. Excess differential growth may cause the retraction of the midgut before the formation of small intestinal loops. The results of this study will serve as reference in future studies on embryology and tissue engineering.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145051491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inverse analysis of patient-specific parameters of a 3D-0D closed-loop cardiovascular model with an exemplary application to an adult tetralogy of Fallot case. 3D-0D闭环心血管模型患者特异性参数的逆分析，并以成人法洛四联症为例。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-09-13 DOI: 10.1007/s10237-025-02006-w

Tahar Arjoune, Christian Bilas, Christian Meierhofer, Heiko Stern, Peter Ewert, Michael W Gee

{"title":"Inverse analysis of patient-specific parameters of a 3D-0D closed-loop cardiovascular model with an exemplary application to an adult tetralogy of Fallot case.","authors":"Tahar Arjoune, Christian Bilas, Christian Meierhofer, Heiko Stern, Peter Ewert, Michael W Gee","doi":"10.1007/s10237-025-02006-w","DOIUrl":"https://doi.org/10.1007/s10237-025-02006-w","url":null,"abstract":"Patient-specific computational models of the cardiovascular system can inform clinical decision-making by providing physics-based, non-invasive calculations of quantities that cannot be measured or are impractical to measure and by predicting physiological changes due to interventions. In particular, mixed-dimensional 3D-0D coupled models can represent spatially resolved 3D myocardial tissue mechanics and 0D pressure-flow relationships in heart valves and vascular system compartments, while accounting for their interactions in a closed-loop setting. We present an inverse analysis framework for the automated identification of a set of 3D and 0D patient-specific parameters based on flow, pressure, and cine cardiac MRI measurements. We propose a novel decomposition of the underlying large, nonlinear, and mixed-dimensional inverse problem into an equivalent set of independently solvable, computationally efficient, and well-posed inverse subproblems. This decomposition is enabled by the availability of measurement data of the coupling quantities and ensures a faster convergence toward a unique minimum. The inverse subproblems are solved with a L-BFGS optimization algorithm and an adjoint gradient evaluation. The proposed framework is demonstrated in a clinical case study of an adult repaired tetralogy of Fallot (ToF) patient with severe pulmonary regurgitation. The identified parameters provide a good agreement between measured and computed flows, pressures, and chamber volumes, ensuring a patient-specific model response. The outcome prediction of an in silico pulmonary valve replacement using the personalized model is physiologically consistent and correlates well with postoperative measurements. The proposed framework is essential for developing accurate and reliable cardiovascular digital twins and exploiting their predictive capabilities for intervention planning.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145051525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing calcification effects in TEVAR procedures: a computational analysis. 评估TEVAR手术中的钙化效应：一项计算分析。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-09-12 DOI: 10.1007/s10237-025-01998-9

Giulia De Campo, Anna Ramella, Sara Barati, Giulia Luraghi, Virginia Fregona, Maurizio Domanin, Robin Heijmen, Santi Trimarchi, Francesco Migliavacca

{"title":"Assessing calcification effects in TEVAR procedures: a computational analysis.","authors":"Giulia De Campo, Anna Ramella, Sara Barati, Giulia Luraghi, Virginia Fregona, Maurizio Domanin, Robin Heijmen, Santi Trimarchi, Francesco Migliavacca","doi":"10.1007/s10237-025-01998-9","DOIUrl":"https://doi.org/10.1007/s10237-025-01998-9","url":null,"abstract":"Thoracic endovascular aortic repair (TEVAR) procedure is sometimes discouraged from clinical guidelines in the presence of calcifications and thrombus along the sealing zones. This computational study aims to understand which is the effect of calcification on stent graft displacement after TEVAR procedure, simulated in a patient-specific anatomy with a penetrating aortic ulcer (PAU).A patient-specific anatomy without calcification is taken as reference, and four models with idealized calcifications positioned in different regions and with different material properties are analyzed. Opening area, von Mises stresses and contact pressures are evaluated to provide a reliable comparison between the calcified (Ca) and the non-calcified models (noCa), and among the calcified models themselves.Comparing qualitatively the Ca and noCa models, no particular changes in the stent graft apposition are observed. In addition, in the Ca models the opening area results lower with respect to the noCa models, but no significant differences are observed among the Ca models. Regarding the von Mises stresses, it seems that the calcifications act as load-bearing structures, absorbing the stresses and reducing them on the aorta. Decreasing the Young modulus of the calcifications, this effect is reduced. Higher contact pressures are observed when the highest Young's modulus of calcification is adopted, with all Ca models having greater pressures than the noCa model.From this analysis, the stent graft seems to be positioned correctly inside the aorta, even in the presence of calcifications. In this setting, the calcifications seem to reduce the stresses on the aorta, thus reducing the likelihood of aneurysm rupture.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D force microscopy for volumetric quantification of ultrasound-induced loading: applications for bone repair. 三维力显微镜用于超声诱导载荷的体积量化：骨修复的应用。

IF 2.7 3区医学

Biomechanics and Modeling in Mechanobiology Pub Date : 2025-09-12 DOI: 10.1007/s10237-025-02009-7

Kevin P Grassie, Fei Wang, Bryan D Huey, Yusuf M Khan

{"title":"3D force microscopy for volumetric quantification of ultrasound-induced loading: applications for bone repair.","authors":"Kevin P Grassie, Fei Wang, Bryan D Huey, Yusuf M Khan","doi":"10.1007/s10237-025-02009-7","DOIUrl":"https://doi.org/10.1007/s10237-025-02009-7","url":null,"abstract":"Mechanical forces on cells and tissues are known to play key roles in regulating cell fate, function, and tissue repair. In bone tissue engineering, mechanical stimulation of cell-hydrogel constructs with low-intensity ultrasound has become a promising therapy for improving the pace and extent of bone regeneration in challenging defects, though its physical and biological mechanisms are not fully understood. In particular, the local ultrasound-induced forces that are imparted to fully encapsulated cells have not been directly quantified. Here, we have developed, validated, and applied a novel 3D force microscopy technique (3D-FM) that extends established principles of unconstrained, regularized, Fourier domain traction force microscopy to reconstruct forces within ultrasound-displaced 3D cell-hydrogel constructs. Validation tests with simulated data demonstrated that the algorithm is capable of reconstructing simple and complex force-density fields from simulated displacements and is robust against corruption with noise. 3D-FM was then used to estimate the ultrasound-induced forces around a bone marrow stromal cell within a soft collagen hydrogel. Localized forces near the cell had magnitudes comparable to other reported cell-scale forces (~ 100 nN), with components both parallel and perpendicular to the direction of ultrasound propagation. This work demonstrates that 3D-FM can elucidate the microscopic physical effects of low-intensity ultrasound on cells in soft matrices used in bone regeneration applications, which can provide valuable insight into the relationship between applied physical forces and cellular responses.","PeriodicalId":489,"journal":{"name":"Biomechanics and Modeling in Mechanobiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0