Applied Biochemistry and Biotechnology最新文献

{"title":"In Vitro Evaluation of Novel Synthetic Benzimidazolium-Chalcone Hybrids: Antioxidant and Regenerative Effects in Diabetic Wound Healing.","authors":"Burcu Demirbağ, Hakan Ünver, Ayça Kara, Adem Necip, Metin Yıldırım","doi":"10.1007/s12010-026-05712-y","DOIUrl":"https://doi.org/10.1007/s12010-026-05712-y","url":null,"abstract":"<p><p>The prevalence of diabetes mellitus (DM) is increasing daily worldwide. DM patients suffer from numerous complications, including the development of chronic wounds that can lead to amputation. These complications necessitate innovative approaches. As part of these innovative approaches, this study synthesized four chalcone derivatives and evaluated their activities in an in vitro diabetic wound model. Several spectroscopic techniques, including 1 H and 13 C NMR and HR-MS, were used to confirm the structures of the newly synthesized compounds. After investigating the inhibition of α-glucosidase in HDF-1 cells treated with D-glucose (50 mM), MTT tests and scratch tests were performed. Tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), total antioxidant status (TAS), and procollagen type-1 were analyzed using an ELISA kit. Additionally, proliferation (Ki67), inflammatory (Nuclear factor kappa B; NFκB), and growth factor (Platelet-derived growth factor subunit A; PDGFA) markers in fibroblasts were assessed by immunohistochemistry. All compounds exhibited strong α-glucosidase inhibitory activity, with IC₅₀ values ranging from 1.115 to 1.612 µg/mL. Cytotoxicity analysis demonstrated that all compounds were biocompatible, maintaining over 85% cell viability in HDF-1 cells. Under diabetic conditions, treatment significantly improved cell viability and promoted wound closure, particularly in C4. In addition, the compounds reduced pro-inflammatory cytokines TNF-α and IL-1β while increasing TAS and procollagen type I levels. Immunocytochemical findings revealed enhanced Ki67 and PDGFA expression and decreased NFκB activation in treated groups. Molecular docking analysis supported the experimental findings by demonstrating favorable binding interactions with α-glucosidase. In conclusion, chalcone derivatives-particularly compound C4-promote diabetic wound healing through multitarget mechanisms involving anti-inflammatory, antioxidant, and pro-regenerative effects, highlighting their strong therapeutic potential.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MXene Decorated Graphitic Carbon Nitride-based Electrochemical Biosensor for the Ultrasensitive Determination of Trichlorfon.","authors":"Diksha, Nishul Khanna, Divya Hudda, Sweety, Ritu Sharma, Devendra Kumar","doi":"10.1007/s12010-026-05717-7","DOIUrl":"https://doi.org/10.1007/s12010-026-05717-7","url":null,"abstract":"<p><p>Organophosphate pesticides have been widely used to protect crops from pests during growth and to maintain their quality after harvest. However, their residues and decomposition products may permeate the soil and water systems, eventually accumulating in food products and posing potential health and environmental concerns. Therefore, the development of a robust analytical technique to monitor these residues is crucial. Herein, a novel and highly efficient acetylcholinesterase (AChE) based electrochemical biosensing platform was constructed using MXene and graphitic carbon nitride (Ti₃C₂T_x@g-C₃N₄) as a substrate material for the rapid determination of trichlorfon (TF). The synergistic effect of Ti₃C₂T_x@g-C₃N₄ not only facilitates electron diffusion at the sensing interface but also enhances the electroactive surface area for AChE immobilization. The structural and morphological features of the Ti₃C₂T_x@g-C₃N₄ composite were characterized using various analytical techniques. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) were employed to examine the electrochemical behaviour of the prepared biosensor. Under optimal conditions, the fabricated biosensor (AChE/M-Ti₃C₂T_x@g-C₃N₄/ITO) demonstrates a wide linear range (0.1 pM-1 µM) and a low detection limit (LOD) of 0.1 pM. Moreover, the biosensor shows good reproducibility, high anti-interference ability, and acceptable stability. Finally, the feasibility of developed biosensor was validated in real matrices (carrot, guava, and fenugreek) which exhibits the satisfactory recovery (91.9%-108.1%).</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citrus limon-Derived Extracellular Vesicles Promote Hair Growth.","authors":"Fatemeh Raoufimanesh, Seyed Mohammad Atyabi, Marjan Mohamadali, Zeinab Alsadat Mirshaby, Shiva Irani, Rassoul Dinarvand","doi":"10.1007/s12010-026-05659-0","DOIUrl":"https://doi.org/10.1007/s12010-026-05659-0","url":null,"abstract":"<p><p>The use of Exosomes (EXOs) to induce the regeneration of hair follicles has received much attention today. The present study was conducted to survey the effects of Citrus limon-derived EXOs (EXO-CLs) on promoting hair growth. EXOs were isolated from citrus limon by the filtration technique. According to NanoSight DynamicLight Scattering and Scanning Electron Microscopy, the extracted EXOs had a size of 147 nm and a spherical morphology. The flow cytometry analysis showed the expression of surface markers CD9 (64.6%), CD63(66.2%), and CD81(65.2%). The MTT test showed a significant increase in the percentage of human skin fibroblast cell viability in the group treated with EXO-CLs (400 µg/mL) compared to the control. The evaluations performed in vivo phase showed a significant increase in the thickness of dermis, the number of hair follicles, and the percentage of collagen were observed in the group treated with EXO compared to the negative control group. The expression ratio of Wnt-1, β-Catenin, Lef1, Vegf, Tgf-β1, and Collagen I genes respectively, increased by a factor of 4.4, 4.8, 2.7, 2.3, 4.6, and 2.4 in EXO-CLs groups compared to the negative control group. According to the findings, the EXO-CLs could promote hair follicle regeneration in vivo and improve hair regrowth.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of Lycium Barbarum Pigment by Surfactant-Assisted Deep Eutectic Solvents and Its Antioxidant Activity.","authors":"Xiumei Guo, Peng Liu, Minsong Zhang, Meijia Song, Manman Shi, Hongzeng Gao, Jiandong Cui","doi":"10.1007/s12010-026-05716-8","DOIUrl":"https://doi.org/10.1007/s12010-026-05716-8","url":null,"abstract":"<p><p>The traditional extraction method of Lycium barbarum pigment has some problems, such as long extraction time, large solvent consumption, low extraction efficiency and serious environmental pollution, so it is necessary to develop a new extraction method with shorter treatment time, lower cost, easier operation and environmental friendliness than the traditional method. In this study, the pigment from Ningxia Lycium barbarum was extracted, purified and identified by deep eutectic solvents assisted by surfactant. The pigments from Lycium barbarum from Ningxia were extracted, purified and identified by surfactant-assisted deep eutectic solvents (DES). The maximal pigments extraction rate was obtained under optimal conditions (3% surfactant Tween 20 (v/v), menthol-lactic acid with water content of 50% (molar ratio of 1:4), 1% material-liquid ratio (m/v), the water bath at 30 ℃ for 2.5 h). The pigments extraction rate by surfactant-assisted DES was 6-folds higher than traditional extraction agents (ethanol), and reached 32.09 mg/g. The HPLC detection results showed that the main component of the pigments was corn lutein dipalmitate with 348.861 mg/g. Furthermore, the purified pigments exhibited excellent free radical scavenging activities for DPPH and ABTS⁺, and ·OH radicals, indicating its high antioxidant activity.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Enzymatic Activity and Stability of Crude Laccase Through Encapsulation in Gelatin/Chitosan Hydrogel.","authors":"Xiang Li, Xuanhe Fu, Baiyu Hui, Xu Li, Zhencheng Su, Huiwen Zhang, Zhixiong Yu","doi":"10.1007/s12010-026-05715-9","DOIUrl":"https://doi.org/10.1007/s12010-026-05715-9","url":null,"abstract":"<p><p>Laccase is an environmentally friendly catalyst characterized by a wide substrate spectrum and clean reaction processes. It demonstrates the ability to efficiently oxidize various pollutants, with water being the sole by-product. Consequently, laccase holds significant potential for applications in diverse fields such as papermaking, environmental protection, food processing, and bioenergy production. In this study, crude laccase produced by Schizophyllum commune was immobilized within a composite material through chemical cross-linking to enhance the enzyme's activity and stability. Free laccase was immobilized using a gelatin/chitosan (GEL/CS) hydrogel, and the conditions for this process were optimized. Enzymatic properties of immobilized laccase (GEL/CS-laccase) were investigated to assess its potential applications. Results indicated that the maximum laccase activity by Schizophyllum commune reached 343.9 U·mL^- 1 on day 6. Following optimization, GEL/CS-laccase achieved an activity recovery of 33.9% and an activity of 113.8 U·g^- 1. Compared with free laccase, GEL/CS-laccase shows better adaptability to pH and temperature. Furthermore, GEL/CS-laccase demonstrates moderate reusability and excellent long-term storage stability: its activity remains above 34.7% after 5 cycles and exceeds 52.9% following 180 days of storage at -80 °C and vacuum. This study has developed an efficient enzyme immobilization method, enhancing its stability and operability, and laying a solid foundation for the practical application of laccase.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric Sensing of Cd(II) Using 4-Fluorobenzohydrazide-Stabilized Silver Nanoparticles and Evaluation of Photodegradation and Antibacterial Properties.","authors":"Ali Usman, Samreen Gul Khan, Azhar Abbas, Akbar Ali, Atta Ul Haq","doi":"10.1007/s12010-026-05710-0","DOIUrl":"https://doi.org/10.1007/s12010-026-05710-0","url":null,"abstract":"<p><p>The alarming contamination of aquatic ecosystems with toxic heavy metals and persistent organic dyes demands innovative nanotechnological solutions for simultaneous detection and remediation. In this research, 4-fluorobenzohydrazide-stabilized silver nanoparticles (4-FBH@AgNPs) were synthesized to serve as a versatile nanoplatform for the colorimetric detection of Cd(II), photocatalytic degradation of the methylene blue (MB) dye, and antimicrobial applications. The synthesized nanoparticles were characterized using various techniques, including UV-Visible spectroscopy, FTIR, XRD, TEM, XPS, DLS, zeta potential, and SEM to assess their structural and optical properties. The UV-Vis analysis displayed a distinct localized surface plasmon resonance (LSPR) peak at 435 nm, confirming nanoparticle formation. Colorimetric sensing tests revealed notable color changes for Cd(II), showing a linear response in the nanomolar range with a low limit of detection (LOD) of 37 nM, demonstrating high sensitivity and selectivity towards Cd(II) ions. Additionally, the 4-FBH@AgNPs exhibited impressive photocatalytic efficiency, achieving over 95% degradation of methylene blue (MB) in 60 min under visible light, attributed to improved electron-hole separation and enhanced charge transfer due to plasmon effects. The nanoparticles also exhibited considerable antibacterial properties against Escherichia coli and Staphylococcus aureus. Statistical analysis affirmed the reproducibility and reliability of the photocatalytic and antimicrobial results by response surface methodology. Collectively, these findings demonstrate that 4-FBH@AgNPs possess excellent multifunctional capabilities, making them a promising candidate for environmental remediation and biosensing applications.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer Potential of Streptomyces sp. Strain M12: Apoptosis Induction and Anti-Migratory Effects in Colorectal Cancer Cells.","authors":"Ehsan Hasanzadeh, Nasrin Motamed, Ensieh Salehghamari, Mahsa Fereidoni, Maryam Taher, Hanieh Jalali","doi":"10.1007/s12010-026-05628-7","DOIUrl":"https://doi.org/10.1007/s12010-026-05628-7","url":null,"abstract":"","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brucea Javanica Oil Attenuates Osteosarcoma Progression by Reprogramming Tumor-associated Macrophages via Inhibiting PI3K/AKT Pathway.","authors":"Yongqi Guo, Yantao Jiang, Zhenlin Li, Jing Zhou, Nan Yao","doi":"10.1007/s12010-026-05713-x","DOIUrl":"https://doi.org/10.1007/s12010-026-05713-x","url":null,"abstract":"","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic Acid-functionalized Hesperidin-loaded Solid Lipid Nanoparticles for Mitigating Oxidative Stress: A Potential Strategy for Radiation-induced Skin Injury.","authors":"Nileshsinh Chauhan, Anshu Kumar, Anupam Jyoti, Pranav Shah, Nimeet Desai, Manisha Lalan","doi":"10.1007/s12010-026-05714-w","DOIUrl":"https://doi.org/10.1007/s12010-026-05714-w","url":null,"abstract":"","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Genomics of Lacticaseibacillus paracasei Reveal the Metabolism of Major Carbohydrates in Soybean Molasses.","authors":"Liping Wu, Linfang Zhang, Sixuan He, Yuanyuan Liu, Shan Wu, Mei Zhang, Guofang Zhang, Yun Zhang, Ekaterina Ivanovna Reshetnik, Svetlana Leonidovna Gribanova, Libo Liu, Song Wang","doi":"10.1007/s12010-025-05567-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05567-9","url":null,"abstract":"","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}