Applied Biochemistry and Biotechnology最新文献

{"title":"Opuntia ficus-indica var. Jalpa (Nopal) Induces Different Cell Death Mechanisms in Colorectal Cancer Cells Depending on the Extraction Method Used.","authors":"María Norma González-Flores, Kenny Misael Calvillo-Rodríguez, Cesar Ivan Romo-Saenz, Cristina Rodríguez-Padilla, Ana Carolina Martínez-Torres, Marilena Antunes-Ricardo","doi":"10.1007/s12010-025-05325-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05325-x","url":null,"abstract":"<p><p>Opuntia ficus-indica (OF) is a native plant of America with biological properties attributed to flavonoids. There are different extraction methods for these compounds; however, it is unknown how different extraction methods might impact the phytochemical concentration ratios and their associated biological effects. Previous studies reported that OF extracts are cytotoxic to colorectal cancer (CRC) cells; however, the cell death mechanism and selectivity to cancer cells have been barely investigated. The objective of this study is to elucidate how different extraction methods impact the phytochemical concentration ratios, the selectivity, and cell death mechanism induced by OF extract in human (Caco-2, HT-29) and murine (CT-26) CRC cells. Extracts were prepared with 80% methanol and 4 N sodium hydroxide, quantification was measured by HPLC-DAD, and identification was measured by LC/MS- TOF. Cell death characteristics were measured by flow cytometry, and its inhibitions were measured by trypan-blue exclusion assay and morphological changes by microscopy. Immune system cell viability was evaluated by MTT. Methanolic extract has a higher yield of phenolic compounds, which correlates with an increased reduction in CRC cell viability at lower concentrations than alkaline, while being non-toxic to immune system cells. Both extracts induced morphological changes, ROS production, ΔΨm loss, and DNA degradation in CRC cell lines. Both extracts induce different cell death modalities in CRC cells. These findings highlight the relevance of OF with significant antitumor activity and demonstrate how the extraction method impacts the compound profile and the observed biological activity and thus, the cytotoxic mechanism.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of Antidiabetic and Anti-Apoptotic Activity of Cissus quadrangularis Stem Through Mechanistic Pathway: An in vitro, in silico and in vivo Approach.","authors":"Shubham Paul, Pronoy Kanti Das, Sakshar Saha, Prasad Sanjay Dhiwar, Ritu Khanra, Atanu Chatterjee, Fernando Berton Zanchi","doi":"10.1007/s12010-025-05338-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05338-6","url":null,"abstract":"<p><p>In the twenty-first century, across the world, there are few ethnomedical solutions for treating type 2 diabetes mellitus (T2DM), a common metabolic disease and its associated disorders. The present study assessed the hypoglycaemic effects of an extract from the stem of Cissus quadrangularis in a rat model with the addition of in vitro and in silico investigations specifically α-amylase, α-glucosidase inhibitory tests and molecular docking, molecular dynamics (MD) simulation study. Streptozotocin and nicotinamide were used to generate type 2 diabetes in rats. Thereafter, Cissus quadrangularis (CQ) stem extract was given orally for 28 days at doses of 200 and 400 mg/kg body weight. The extract improved biochemical indicators, lowered lipid peroxidation, raised HDL levels, and decreased blood glucose in a dose-dependent manner. Tissue healing in treated groups was confirmed by histopathological examination. The α-amylase and α-glucosidase inhibition assays had respective IC₅₀ values of 50.18 ± 2.67 µg/ml and 50.93 ± 1.79 µg/ml which are pretty much comparable with the standard, acarbose. By measuring apoptotic proteins such as BAX, BCL2, caspase 3, and caspase 9, the results show that the extract has a considerable effect on preventing cell death. The introduction of CQ stem extract to the experimental animals elevates the glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT), suppressing the amount of thiobarbituric acid relative substance (TBARS) in liver tissue. These findings from in vitro, in vivo, and in silico research suggest the potential of CQ stem extract as a preventive measure against apoptosis and an alternative therapeutic strategy for type 2 diabetes by activating the adenosine 5'-monophosphate/phosphatidylinositol 3-kinase/protein kinase B (AMPK/PI3K/AKT) signalling pathway.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship Between the Gut Microbiome and the Aqua Cultured Fish, General Prospects Toward Fish Health: A Systematic Review.","authors":"Darren Dean Tay, Vijay Subbiah Kumar, Rossita Shapawi, Muhammad Dawood Shah, Hajar Fauzan Ahmad, Nurzafirah Mazlan","doi":"10.1007/s12010-025-05330-0","DOIUrl":"https://doi.org/10.1007/s12010-025-05330-0","url":null,"abstract":"<p><p>Aquaculture allows the cultivation of aquatic life outside its normal origins which can provide work opportunities, seafood security, as well as conservation efforts for endangered fish species. Numerous factors influence the health of aquaculture fish, with the gut microbiome playing a pivotal role. Research indicates that an imbalance or dysbiosis in the gut microbiome can significantly affect the overall well-being and health outcome of these fish. Despite extensive research utilizing metagenomics across diverse environments and controlled conditions, a clear consensus on the characteristic of \"healthy\" or \"optimal\" gut microbiome in domesticated fish has yet to be established. This review will cover 28 studies, which further discusses the findings of the gut microbiome within fish and attempts to provide a general outline of how the gut bacteria may interact and affect fish health within aquaculture environments. The indices as well as pathogens and beneficial bacteria of each study are also listed. This review aims to provide readers with an enhanced understanding of the complex dynamics of the gut microbiome in aquaculture fish, while offering insights that could inform the design of future studies in this field.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Profiling of Cow Urine of Various Breeds Reveals Bioactive Metabolites of Diverse Industrial Applications.","authors":"Jeetesh Kushwaha, Yashpal Singh, M S Mahesh, Sushil Kumar Yadav, Pratik N Sheth, Abhishek S Dhoble","doi":"10.1007/s12010-025-05300-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05300-6","url":null,"abstract":"<p><p>Cow urine is widely utilized for medicinal and agricultural purposes in rural areas of India, with urine from indigenous cow breeds (Bos indicus) believed to offer unique benefits compared to that of exotic (Bos taurus) breeds. This research aimed to profile the metabolites present in the urine of indigenous breeds of cows using gas chromatography-mass spectrometry (GC-MS) and to explore the potential applications of the identified compounds by referencing the established literature. The various cow breeds included in the study were Gir, Sahiwal, Gangatiri, Hariana, Kankrej, Rathi, Gaolao, and Jersey. Cows employed in the study to collect the samples from various locations differed in their body weight, age, and stage of lactation. GC-MS analysis revealed a range of compounds, including ethanone, cresol, bis(2-ethylhexyl) phthalate, phenol, eicosane, pentanol, isobutyl ester, ethyl ester, binapacryl, trifluoroacetate, xylene, amylene hydrate, dibutyl ester, and formamide. Notably, several compounds were consistently observed across multiple indigenous breeds. For instance, bis(2-ethylhexyl) phthalate and xylene were found in nearly all indigenous breeds, while ethanone was detected in Gir, Sahiwal, Gangatiri, Kankrej, Hariana, Gaolao as well as Jersey cows. Similarly, eicosane and pentanol were present in Gangatiri and Hariana breeds. These overlapping chemical signatures highlight potential metabolic similarities among the studied cow breeds. The identified compounds are known for their diverse industrial and pharmaceutical applications, including use in disinfectants, flavorings, cosmetics, and agrochemicals as well as metabolic engineering. Thus, this study-for the first time-comprehensively delineated the comparative metabolite profile of cow urine among different breeds of cows. The spectrum of urinary metabolites identified could offer opportunities to foster bio-based innovations having multifarious applications, including new product developments, across diversified fields.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms and Research Methods of Protein Modification in Virus Entry.","authors":"Yuyang Xiao, Mingyang Gao, Xianqi Mo, Jiamiao Lang, Zimeng Wang, Zhongjun Ma, Meng Yang, Bailu Tang, Dan Liu, Hailun He","doi":"10.1007/s12010-025-05333-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05333-x","url":null,"abstract":"<p><p>Protein interactions are of paramount importance for the performance of biological functions within organisms. Post-translational modifications, including glycosylation and phosphorylation, regulate protein-protein interactions through non-covalent mechanisms. Glycosylation typically facilitates binding by altering surface properties, whereas phosphorylation can either enhance or disrupt interactions depending on context, collectively amplifying the biological impact of proteins. The entry of viruses and certain intracellular parasites into host cells is facilitated by these modifications, which permit the binding of ligands to receptors and the traversal of the cell membrane barrier. As research in this domain progresses, innovative methodologies are being developed, including protein microarrays and proximity-labeling techniques. These developments are being increasingly employed in disease prevention, therapeutics, and fundamental medical research. In light of the recent surge in emerging infectious diseases, the study of protein interactions has assumed heightened relevance. This review explores protein modifications, including glycosylation, phosphorylation, and ubiquitination, and focuses on their roles in viral entry. It highlights advanced methods for analyzing protein-protein interactions (PPIs), notably proximity labeling and protein microarrays, and concludes with novel insights into therapeutic development, aiming to inspire innovation in this evolving field.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SMURF1 Affects Dental Implant Integration in Diabetes By Regulating PKG2 Expression.","authors":"Xue-Qin Wei, Sheng-Zhi Zhang","doi":"10.1007/s12010-025-05326-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05326-w","url":null,"abstract":"<p><strong>Background: </strong>Poor dental implant has been reported in type II diabetes mellitus (T2DM), which seriously affects the patients' quality of life. The protein kinase type 2 (PKG2) is a target to regulate osteogenic differentiation. We aimed to investigate the effect and mechanisms of SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1, E3 ubiquitin ligase) on bone integration of dental implants in T2DM by regulating PKG2 in a ubiquitination-dependent manner.</p><p><strong>Methods: </strong>The dental implant model of diabetic rats and the high-glucose-induced bone marrow mesenchymal stem cell (BMSC) model were constructed. The changes in osteogenic differentiation indices were determined by immunohistochemical staining and alizarin red staining. The levels of insulin and glucose tolerance were assessed by glucose and insulin tolerance tests. Hematoxylin-eosin staining was performed to detect alveolar bone inflammation. PKG2, PERK, CHOP, SMURF1, p-PERK, GRP78, and ATF4 levels were examined via quantitative real-time PCR or western blot. The interaction between SMURF1 and PKG2 and PKG2 ubiquitination was analyzed by co-immunoprecipitation.</p><p><strong>Results: </strong>The T2DM model rats exhibited enhanced glucose tolerance and insulin resistance. PKG2 expression and Runx2 signal were reduced in T2DM rats following the dental implant. After treatment with high glucose in BMSCs, the expression of PKG2 and osteogenic differentiation were reduced. The proteasome inhibitor MG132 recovered PKG2 expression. The reduction of PKG2 was related to ubiquitination. SMURF1 is the key E3 ubiquitin ligase for PKG2. SMURF1 expression was increased in T2DM rats and BMSC cells induced by high glucose. Overexpression SMURF1 was downregulated the expression of PKG2 protein. SMURF1 regulated PKG2 expression via ubiquitination. PKG2 overexpression reversed the inhibition of osteogenic differentiation by SMURF1 overexpression, and inhibits the expression of endoplasmic reticulum stress related proteins. Additionally, these changes caused by SMURF1 overexpression were reversed by cinaciguat (PKG2 generator).</p><p><strong>Conclusions: </strong>In T2DM, SMURF1 regulated PKG2 expression through ubiquitination, thereby interfering with osteogenic differentiation and affecting the integration of dental transplantation.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"USP7 Stabilizes USF1 to Aggravate ox-LDL-Induced Endothelial Injury Through the MYD88/NF-κB Pathway in Atherosclerosis.","authors":"Jing Liu, Xiangyang Zhang, Zhaoxia Yu, Tieliang Zhang","doi":"10.1007/s12010-025-05312-2","DOIUrl":"https://doi.org/10.1007/s12010-025-05312-2","url":null,"abstract":"<p><p>Atherosclerosis (AS) is a complex disease that involves the accumulation of lipids in the arterial wall, leading to vessel narrowing and increased risk of heart disease. Upstream stimulatory factor 1 (USF1) is an important regulatory factor that plays an important role in disease progression. Understanding the role and mechanism of USF1 in AS is crucial for unraveling the molecular underpinnings of this condition. Oxidized low-density lipoprotein (Ox-LDL) was used to stimulate human umbilical vein endothelial cells (HUVECs) to induce an AS-like cellular injury. Protein expression was evaluated using western blotting, while mRNA expression was assessed via quantitative real-time polymerase chain reaction. Cell viability and proliferation were analyzed using the cell counting kit-8 and 5-ethynyl-2'-deoxyuridine assays, respectively. Cell apoptosis was examined through flow cytometry. Angiogenic capacity was assessed by tube formation assay in human umbilical vein endothelial cells (HUVECs). Enzyme-linked immunosorbent assays were conducted to measure IL-6 and TNF-α levels, and MDA levels were determined using a lipid peroxidation MDA assay kit. SOD activity was measured using an SOD activity assay kit. Co-immunoprecipitation assay was performed to investigate the association between ubiquitin-specific peptidase 7 (USP7) and USF1, while dual-luciferase reporter assay and chromatin immunoprecipitation assay were conducted to identify the association between USF1 and myeloid differentiation primary response 88 (MYD88). USF1 expression was upregulated in AS patients when compared with healthy volunteers. Knockdown of USF1 protected HUVECs from injury induced by ox-LDL. USP7 was found to stabilize USF1 protein expression by deubiquitination, and its knockdown mitigated ox-LDL-induced HUVEC injury by reducing USF1 protein expression. USF1 was shown to transcriptionally activate MYD88 in HUVECs, and silencing of USF1 protected HUVECs from ox-LDL-induced injury by inhibiting MYD88 expression. Furthermore, USF1 knockdown inactivated the NF-κB pathway by suppressing MYD88 expression. USP7-dependent stabilization of USF1 exacerbated ox-LDL-induced injury in HUVECs by activating the MYD88/NF-κB pathway. These findings underscore the importance of the USP7-USF1-MYD88 axis in AS and suggest potential therapeutic targets for diseases related to AS.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulated PCSK9 Level Contributes to Human Pulmonary Microvascular Endothelial Cell Apoptosis in Cigarette Smoke Extract-Induced COPD Models Through TLR4/NF-kappaB Pathway Activation.","authors":"Yunxia Li, Fengzhen He, Shasha Zhao, Chunli Zhang, Xueyang Chen, Xiang Luo","doi":"10.1007/s12010-025-05328-8","DOIUrl":"https://doi.org/10.1007/s12010-025-05328-8","url":null,"abstract":"<p><p>Chronic obstructive pulmonary disease (COPD) is persistent and can result in irreversible alveolar collapse and increase the risk of cardiovascular disease. PCSK9 belongs to the pro-protein convertase family and is capable of modulating cholesterol metabolism and low-density lipoprotein (LDL). PCSK9 is associated with endothelial dysfunction and its suppression is conducive to vascular function. The aim of this study was to explore PCSK9 expression in the lung tissues of patients with COPD and investigate the regulation of PCSK9 in cigarette smoke extract (CSE)-exposed human pulmonary microvascular endothelial cells (HPMECs) and an in vivo cigarette smoke (CS)-exposed mouse model. PCSK9 is drastically upregulated in the lung tissues of patients with COPD relative to those of individuals who have never smoked. PCSK9 expression in HPMECs and the CS-exposed mouse model was found to increase. Functional assays demonstrated that PCSK9 silencing decreased CS-induced lung injury and neutrophil and macrophage infiltration. PCSK9 silencing also abolished CSE-induced apoptosis by upregulating Bcl-2 and downregulating Bax expression in COPD mice and cell models. PCSK9 silencing alleviated the inflammatory response in the BALF of CS-exposed mice and CSE-treated HPMECs. The protein expression of P65, NLRP3, ASC, TLR4, p-P65, MyD88, and caspase-1 in mouse BALF and HPMECs was inhibited after PCSK9 knockdown. Collectively, these observations indicate the important role of PCSK9 in COPD progression and present promising treatment targets for COPD.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astragalus Polysaccharides Alleviate Sepsis by Boosting Adrenal Hormone Synthesis and Repairing Microvascular Damage.","authors":"Yihui Chai, Yunzhi Chen, Zhaoxia Huang, Xiaoqing Liu, Jun Li","doi":"10.1007/s12010-025-05332-y","DOIUrl":"https://doi.org/10.1007/s12010-025-05332-y","url":null,"abstract":"<p><p>Sepsis is a severe infection-related condition commonly seen in clinical practice, characterized by systemic inflammation and multi-organ dysfunction. Astragalus polysaccharides (APS), the primary bioactive compound from the traditional Chinese herb Astragalus, has shown significant anti-inflammatory, immunomodulatory, and antioxidant effects. This study aimed to explore APS's potential in alleviating sepsis by enhancing adrenal cortical hormone production and repairing adrenal microvascular damage, while uncovering the underlying molecular mechanisms. Using an SD rat model, sepsis was induced via cecal ligation and puncture (CLP) surgery. Rats were treated with APS at doses of 400, 600, or 800 mg/kg/day for 7 days. Survival rates, inflammatory cytokine levels, adrenal function, and molecular pathways were evaluated using techniques such as ELISA, HE staining, TUNEL assay, metabolomics, transcriptome sequencing, and validation. Results showed that APS significantly reduced sepsis-induced mortality, elevated serum cortisol levels, and lowered inflammatory cytokines IL-6 and TNF-α. Histological analysis revealed that APS protected the adrenal cortex from apoptosis and inflammatory infiltration. Metabolomic analysis identified 154 differentially regulated metabolites, including significant changes in steroid hormones. Transcriptomic sequencing indicated changes in gene expression, suggesting that APS may inhibit NF-κB activity and promote nitric oxide (NO) production, thus protecting endothelial cells. These findings suggest that APS improves adrenal function and mitigates sepsis-related damage, offering promising therapeutic avenues for sepsis treatment.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposomal Delivery of Allolobophora caliginosa Coelomic Fluid Attenuates Myocardial Infarction by Suppressing Oxidative Damage, Inflammation, and Apoptosis.","authors":"Asmaa E Farouk, Sohair R Fahmy, Amel M Soliman, Sherif Abdelaziz Ibrahim, Shimaa A Sadek","doi":"10.1007/s12010-025-05340-y","DOIUrl":"https://doi.org/10.1007/s12010-025-05340-y","url":null,"abstract":"<p><p>Myocardial infarction (MI) is a concerning coronary heart disease with increasing rates of death and morbidity worldwide. One potential approach to prevent MI involves exploring invertebrate supplements within the nanoliposome formulation to improve targeted delivery, thereby mitigating MI-induced heart damage. Therefore, the study aimed to evaluate the cardioprotective efficacy of liposomal delivery of Allolobophora caliginosa coelomic fluid (ACCF-liposomes) on adrenaline-induced MI in rats. Thirty male albino rats were allocated into five groups: Control, Untreated MI, MI-treated ACCF, MI-treated free liposomes, and MI-treated ACCF-liposomes. The treatment regimen spanned 21 days. Electrocardiography (ECG), biochemical, oxidative stress, inflammatory mediators, electrolyte balance, histopathological and immunohistochemical analyses, and DNA fragmentation were evaluated. Liposomal delivery of ACCF has shown promise in regulating ECG criteria and reducing myocardial markers, particularly AST, LDH, MMP-2, creatine kinase, and troponin-I. It also improves lipid metabolism and inhibits myocardial oxidative stress. Additionally, ACCF and ACCF-liposomes treatment improves cardiomyocyte architecture and reduces DNA fragmentation in myocardial infarcted rats. Furthermore, encapsulating ACCF within liposomes statistically reduced the expression of iNOS and Beclin-1 in cardiac tissue. This suggests that liposomal delivery of ACCF enhances its effectiveness in treating myocardial infarction, potentially via its antioxidant, anti-inflammatory, and anti-apoptotic attributes.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}