Applied Biochemistry and Biotechnology最新文献

miR-128-3p Reduces Proliferation and Immune Escape in Acute Myeloid Leukemia Through Targeted Regulation of ZEB1. miR-128-3p通过靶向调节ZEB1减少急性髓系白血病的增殖和免疫逃逸。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-17 DOI: 10.1007/s12010-025-05255-8

YanBin Zhang, DanDong Ma, XiaoJuan Zhang, WenKun Chen, XueJiao Wang, Rui Sun, KuiXing Li

{"title":"miR-128-3p Reduces Proliferation and Immune Escape in Acute Myeloid Leukemia Through Targeted Regulation of ZEB1.","authors":"YanBin Zhang, DanDong Ma, XiaoJuan Zhang, WenKun Chen, XueJiao Wang, Rui Sun, KuiXing Li","doi":"10.1007/s12010-025-05255-8","DOIUrl":"https://doi.org/10.1007/s12010-025-05255-8","url":null,"abstract":"microRNAs have received wide attention as potential therapeutic targets. This study explored the action of miR-128-3p in acute myeloid leukemia (AML). miR-128-3p expression in AML was determined by quantitative PCR method. MTT proliferation assay and immunoblot assay were employed to detect proteins related to proliferation and apoptosis in THP-1 cells overexpressing miR-128-3p. RNA immunoprecipitation and dual luciferase reporting system were utilized to verify downstream targets of miR-128-3p. Flow cytometry was conducted to analyze the apoptosis rate and immune escape of THP-1 cells in the T-cell co-culture system. miR-128-3p was lowly expressed in AML patients (reduced by 41.6%). Overexpression of miR-128-3p inhibited THP-1 cell proliferation and immune escape, and stimulated apoptosis. ZEB1 was a downstream target of miR-128-3p, and up-regulation of miR-128-3p inhibited ZEB1 mRNA and protein expression (respectively reduced by 65.8% and 42.0%). Upregulating ZEB1 reversed the inhibitory effect of upregulating miR-128-3p on THP-1 cell proliferation and immune escape. Upregulating ZEB1 promoted PD-L1 protein expression (increased by 0.75-fold). Blocking PD-L1 reversed the promotion of THP-1 cell proliferation and immune escape by upregulating ZEB1. The miR-128-3p/ZEB1/PD-L1 axis is involved in regulating the proliferation and immune escape of AML cells, providing new insights into the molecular mechanism of miR-128-3p in AML and, more importantly, a new target for immunotherapy of AML.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and Formulation of an Emulgel Based on Clove Oil in Order to Evaluate Its Antibacterial and Anti-inflammatory Potential In Vitro and In Vivo for Topical Application. 丁香油乳状液的优化与配方，以评价其体外和体内外用抗菌和抗炎作用。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-17 DOI: 10.1007/s12010-025-05273-6

Lamia Bennabi, Faiza Debab, Faiza Bennabi, Ilham Abdelmalek, Kaddour Guemra, Lahcen Belarbi, Abdelkader Dehbi, Ali Alsalme, Massimo Messori

{"title":"Optimization and Formulation of an Emulgel Based on Clove Oil in Order to Evaluate Its Antibacterial and Anti-inflammatory Potential In Vitro and In Vivo for Topical Application.","authors":"Lamia Bennabi, Faiza Debab, Faiza Bennabi, Ilham Abdelmalek, Kaddour Guemra, Lahcen Belarbi, Abdelkader Dehbi, Ali Alsalme, Massimo Messori","doi":"10.1007/s12010-025-05273-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05273-6","url":null,"abstract":"Inflammation is a significant physiological response of the body to various stimuli, necessitating effective therapeutic interventions. This study focuses on the formulation and evaluation of an emulgel using clove oil, known for its important anti-inflammatory properties. The emulgel was developed by incorporating clove oil into a gel based on the presence of alginate, aiming to enhance its topical delivery and therapeutic efficacy. The formulation process involved optimizing the oil-to-gel ratio for a good consistency, stability and skin permeability. The emulgel was characterized by various physicochemical evaluations, including pH, viscosity, spreadability and stability studies, to ensure its suitability for topical application. In vitro and in vivo anti-inflammatory activity was assessed using clove oil as the active agent in the emulgel in comparison with 1% sodium diclofenac. The study of the denaturation of the BSA protein is in line with the anti-inflammatory study in vivo, where a significant reduction in inflammation has been noted. The study of the antibacterial properties of clove oil, and the emulgel showed that the clove oil with this formulation offers a novel and effective alternative to conventional treatments. It is a very good alternative to other topical forms since the bactericidal character of Gram-positive and Gram-negative strains has been inhibited and presents a high sensitivity to the oil once integrated. This result has been confirmed by the evaluation of the minimum inhibitory concentration; the emulgel for the E. coli strain (Gram-negative bacteria) is 12.6 mg/ml (MIC), and the minimum bactericidal concentration (MBC) of the emulgel is therefore relatively high at 50 mg/ml.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Double-Flapped Dumbbell Probe Functionalized with Silver Nanoclusters for Sensitive Fluorometric Detection of miRNA. 银纳米团簇功能化的双襟翼哑铃探针用于敏感的miRNA荧光检测。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-17 DOI: 10.1007/s12010-025-05264-7

Xiuju Zhang, Xiaowei Nie, Wenxiao Yu, Guanchao Du, Shaoming Liu, Yewen Song

{"title":"Double-Flapped Dumbbell Probe Functionalized with Silver Nanoclusters for Sensitive Fluorometric Detection of miRNA.","authors":"Xiuju Zhang, Xiaowei Nie, Wenxiao Yu, Guanchao Du, Shaoming Liu, Yewen Song","doi":"10.1007/s12010-025-05264-7","DOIUrl":"https://doi.org/10.1007/s12010-025-05264-7","url":null,"abstract":"MicroRNA (miRNA) has emerged as a potential diagnostic marker for malignancies, such as prostate cancer; yet, few analytical approaches have been established for the simple and effective identification and quantification of miRNA. We depict here a fluorescent dumbbell probe utilizing DNA-silver nanoclusters (DNA-AgNCs) to facilitate the simple and label-free quantification of miRNA. The fluorescent probe was methodically engineered using a double-flapped dumbbell structure, wherein the 5' flap was synthesized using DNA-AgNCs, and the 3' flap was extended by a G-rich sequence. The DNA scaffold rigidifies the DNA-AgNCs and the G-rich sequence, bringing them into close contact, which leads to enhanced fluorescence due to the activation of DNA-AgNCs by the G-rich sequence. Upon the introduction of miRNA, the 5' flap of the probe unfolds, liberating the G-rich sequence from the probe, disrupting the closeness between DNA-AgNCs and the G-rich region, and resulting in decreased fluorescence. The proposed method, by incorporating target recycling and polymerase/endonuclease-assisted cycles, enables sensitive detection of miRNA with a detection limit of 4.2 fM and demonstrates high specificity for monitoring target miRNA in clinical samples, offering a robust platform for miRNA monitoring and disease diagnosis.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of the Foxo3/Txnip Axis Alleviates Ventilator-Induced Diaphragmatic Dysfunction by Downregulating MuRF1. Foxo3/Txnip轴抑制通过下调MuRF1减轻呼吸机诱导的膈功能障碍

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-16 DOI: 10.1007/s12010-025-05261-w

Jia Shen, Xiaojun Ma

{"title":"Inhibition of the Foxo3/Txnip Axis Alleviates Ventilator-Induced Diaphragmatic Dysfunction by Downregulating MuRF1.","authors":"Jia Shen, Xiaojun Ma","doi":"10.1007/s12010-025-05261-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05261-w","url":null,"abstract":"Ventilator-induced diaphragm dysfunction (VIDD) is one of the main causes of weaning from mechanical ventilation (MV). The forkhead box O3 (Foxo3) has been identified as being involved in regulating the contractile function of skeletal muscle. This study aimed to figure out the regulatory role and mechanism of Foxo3 on VIDD. The mouse myoblast C2C12 cells were stimulated using different intensities of stress to mimic the in-vitro VIDD model. 3- (4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and TdT-mediated dUTP nick end labeling (TUNEL) assays were applied to check cell viability and apoptosis, respectively. Cellular inflammation and oxidative stress levels were evaluated by measuring cellular inflammatory factors (IL-1β and TNF-α) and oxidative stress markers (SOD and MDA). The release of oxygen species (ROS) was assayed by cellular immunofluorescence. The expression of apoptosis-associated proteins (Bax and Bcl-2), Gpx4, Slc7a11, Ptgs2, Foxo3, Txnip, Murf1, Atrogin-1, Nlrp3, Asc, and Caspase1 was gauged using Western blot. The rats with or without MV therapy were treated with the Foxo3 inhibitor Carbenoxolone (CBX) to characterize the impact of Foxo3 on VIDD. Stress stimulation dampened myogenic cell viability, boosted apoptosis, inflammation, oxidative stress, and ROS release, and activated the expression of Foxo3 and Txnip pathways. Overexpression of Txnip or Murf1 lessened the protective effect of FOxO3 inhibition on myoblasts. Downregulation of Txnip or Murf1 mitigated myoblasts dysfunction that was induced by Foxo3 overexpression. In vivo, inhibition of Foxo3 mitigated MV-induced diaphragmatic atrophy and reduced contractility, inflammation, and oxidative stress in rats. Inhibition of Foxo3 eased VIDD by downregulating Txnip and Murf1.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced bioethanol Production from Wheat Bran Feedstock by a Mild Oxalic Acid Pretreatment. 温和草酸预处理强化麦麸原料生产生物乙醇。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-16 DOI: 10.1007/s12010-025-05281-6

Xiaohong Yu, Ya Zhang, Xianhao Wang, Yutong Luo, Shuai Shao, Zhongyang Qiu

{"title":"Enhanced bioethanol Production from Wheat Bran Feedstock by a Mild Oxalic Acid Pretreatment.","authors":"Xiaohong Yu, Ya Zhang, Xianhao Wang, Yutong Luo, Shuai Shao, Zhongyang Qiu","doi":"10.1007/s12010-025-05281-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05281-6","url":null,"abstract":"Organic acid pretreatment is one of the most promising methods to pretreat lignocellulose biomass due to its mild condition and weak corrosion to reactor. In this study, five different organic acids were used for wheat bran pretreatment, and the enzymatic hydrolysis yields of pretreated feedstocks were evaluated. Among these organic acids, oxalic acid pretreatment demonstrated the highest glucose yield of 87.93%. Subsequently, the optimization of oxalic acid pretreatment parameters was conducted including temperature, time, solid-liquid ratio, and acid usage. A high glucose yield of 96.92% was obtained at the optimal conditions: 0.8% of oxalic acid usage, 1:10 of solid-liquid ratio, and 130 ℃ for 15 min. FTIR, XRD and SEM revealed the mechanism of improved hydrolysis efficiency after oxalic acid pretreatment. Finally, the pretreated wheat bran was used for separate hydrolysis and ethanol fermentation (SHF) at 20% (w/w) solids loading. After 36 h, 23.87 g/L ethanol was produced by Saccharomyces cerevisiae DQ1 with all glucose consumed, with the yield of 0.12 g/g dry virgin wheat bran. This study provided a new insight into wheat bran pretreatment for biofuel production by a mild oxalic acid pretreatment method.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the Thermostability of Phospholipase C by Structural-Based Proline Incorporation to Improve Its Degumming Performance. 结构基脯氨酸掺入提高磷脂酶C的热稳定性以改善其脱胶性能。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-15 DOI: 10.1007/s12010-025-05271-8

Yiling Tang, Zhiwei Lin, Lei Ren, Chenhao Feng, Yonghua Wang, Fanghua Wang

{"title":"Enhancing the Thermostability of Phospholipase C by Structural-Based Proline Incorporation to Improve Its Degumming Performance.","authors":"Yiling Tang, Zhiwei Lin, Lei Ren, Chenhao Feng, Yonghua Wang, Fanghua Wang","doi":"10.1007/s12010-025-05271-8","DOIUrl":"https://doi.org/10.1007/s12010-025-05271-8","url":null,"abstract":"Thermostability can be improved by introducing prolines into targeted sites, enhancing enzyme performance in specific reactions. In our present study, a novel fungal phospholipase C derived from Talaromyces islandicus (TiPLC) was first heterologously expressed in Pichia pastoris and biochemically characterized. Given the poor thermal stability of TiPLC, a structure-based proline incorporation strategy was used to enhance its thermostability further. Two single-site (E92P and A375P) mutants were selected from seven designs, exhibiting improved stability while retaining wild-type's basic properties (optimum reaction pH and temperature). Compared to the wild-type, the t1/2 of E92P and A375P under 40 °C extended by 1.62 and 1.27 times, respectively. Meanwhile, the E92P and A375P mutants exhibited a 20% increase in activity using p-NPPC as substrate. Moreover, double mutant E92P-A375P exhibited 2.43 times enhancement compared to the wild-type. Results of the oil degumming experiment further confirmed that the double mutant significantly improved the performance of TiPLC, with a reduction in residual phosphorus to 78 ppm, while for the wild-type, the residual phosphorus was 131 ppm under the same reaction. Molecular simulations indicated that proline incorporation into 92 and 375 sites significantly improved the rigidity of partial flexible regions, thus contributing to the increased thermostability.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic Properties of Cel5B and Cel7 A-2 from Penicillium oxalicum and Their Role in the Enzymatic Saccharification of Lignocellulose. 草青霉Cel5B和cel7a -2的酶学性质及其在木质纤维素酶糖化中的作用。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-15 DOI: 10.1007/s12010-025-05285-2

Wenxia Song, Xianqin Lu, Xiaolong Han, Yinbo Qu

{"title":"Enzymatic Properties of Cel5B and Cel7 A-2 from Penicillium oxalicum and Their Role in the Enzymatic Saccharification of Lignocellulose.","authors":"Wenxia Song, Xianqin Lu, Xiaolong Han, Yinbo Qu","doi":"10.1007/s12010-025-05285-2","DOIUrl":"https://doi.org/10.1007/s12010-025-05285-2","url":null,"abstract":"Cellulase cocktails play a crucial role in enzymatic saccharification of lignocellulosic biomass, a critical step in sustainable biofuel production. However, the efficiency of cellulase cocktails remains suboptimal due to incomplete understanding of enzyme synergy. In this study, we aimed to enhance biomass hydrolysis by optimizing the synergistic action of recombinant Cel5B, an endoglucanase and Cel7A-2, cellobiohydrolase from Penicillium oxalicum within a reconstituted cellulase system. Both enzymes were successfully expressed, purified, and characterized to optimize their synergistic action. The enzymatic properties of both enzymes were assessed, revealing optimal activities at 55-65°C and pH 4.2-4.8. Their combined action significantly enhanced the hydrolysis of filter paper and corncob residues. Using a central composite design, enzyme ratios were optimized to 11.6% rCel7A-2, 23.7% rCel5B, and 31.7% β-glucosidase. This formulation achieved the predicted glucan conversion of 58.1% for corncob residue hydrolysis, with experimental validation yielding 57.2%, demonstrating a 98.5% agreement with the model. Compared to the that of the commercial cellulase produced from P. oxalicum (38.1%), the optimized process improved glucan conversion by 50.1%. These findings demonstrate the effectiveness of rational enzyme synergy optimization and provide valuable insights into the strategy for improving the lignocellulosic biomass saccharification efficiency of the cellulase system.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Microbial Alkaline Proteases: Addressing Industrial Bottlenecks Through Genetic and Enzyme Engineering. 微生物碱性蛋白酶的研究进展：通过基因工程和酶工程解决工业瓶颈。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-15 DOI: 10.1007/s12010-025-05270-9

Nitin Srivastava, Sunil Kumar Khare

{"title":"Advances in Microbial Alkaline Proteases: Addressing Industrial Bottlenecks Through Genetic and Enzyme Engineering.","authors":"Nitin Srivastava, Sunil Kumar Khare","doi":"10.1007/s12010-025-05270-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05270-9","url":null,"abstract":"Microbial alkaline proteases are versatile enzymes chiefly employed in various industrial sectors, viz., food processing, detergents, leather, textile, pharmaceutical industries. However, the existing bottlenecks, such as lower enzyme yields, stability, purification, specificity, and catalytic rates, bring resistance toward their industrial suitability. The robust microbes are prominent sources of stable enzymes. However, further challenges may exist, such as low yield, difficult purification, and lesser enzymatic efficiency. With the advent of advanced genomic and enzyme engineering approaches, such bottlenecks can be overcome. Initially, the microbial genomes can be used as novel repositories for stable enzyme sequences for further heterologous production with higher enzymatic yields and an easier purification process. Moreover, enzyme improvement through directed evolution and rational engineering could enhance enzyme stability and efficiency. Currently, conventional enzyme improvement methods are increasingly replaced by Artificial Intelligence-Machine Learning (AI-ML) and computational data-driven tools that provide precise information for tailoring enzymes for industrial endeavors. Hence, the current review encompasses a deliberate study of microbial alkaline proteases, their major industrial applications, and the bottlenecks in their commercial implementations. Further, it presents in-detailed solutions, including genetic and enzyme engineering, and insights toward incorporating advanced tools like AI-ML and de novo enzyme engineering to subside the existing challenges.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Riboflavin Production by an Enhanced Purine Salvage Pathway in Bacillus subtilis. 通过增强嘌呤回收途径增强枯草芽孢杆菌核黄素生成。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-15 DOI: 10.1007/s12010-025-05254-9

Xi Chen, Youguo Yuan, Xuedong Wang

{"title":"Enhancement of Riboflavin Production by an Enhanced Purine Salvage Pathway in Bacillus subtilis.","authors":"Xi Chen, Youguo Yuan, Xuedong Wang","doi":"10.1007/s12010-025-05254-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05254-9","url":null,"abstract":"Recombinant Bacillus subtilis is currently used as a producer for riboflavin biosynthesis. In this study, a novel metabolic engineering strategy for the enhancement of purine salvage pathway in B. subtilis effectively improved the riboflavin biosynthesis by increasing the supply of precursors via an energy-saving route. Mutant strains overexpressing the related genes of salvage pathway exhibited higher production compared to the original strain. Specifically, the deoD-hprT co-overexpressing mutant BR-08 demonstrated a significant increase in riboflavin production, from 1049.84 to 1252.34 mg/L, representing a 19.29% improvement. To further amplify the impact of the salvage pathway, xylose was introduced into the fermentation process as an inducer to upregulate the expression of the relevant genes, while guanosine was added as a direct precursor. Following the optimization of the fermentation conditions, the riboflavin titer of mutant BR-08 reached 1898.58 mg/L. These findings substantiate the efficacy of the purine salvage pathway in enhancing riboflavin biosynthesis and offer a novel strategy for improving biosynthetic efficiency through an energy-conserving mechanism.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Analysis of the Lysine Acetylome in Macrophages from Salt-sensitive Hypertensive Rats. 盐敏感高血压大鼠巨噬细胞赖氨酸乙酰酶的整体分析。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-15 DOI: 10.1007/s12010-025-05265-6

Di Xie, Yanghong Dong, Jinyu Chi, Wanlin Li, Chunnan Liu, Yang Xu, Yang Li, Jingzhi Wang, Jinfeng Wu, Rui Wang, Kelaier Yang, Xinhua Yin

{"title":"Global Analysis of the Lysine Acetylome in Macrophages from Salt-sensitive Hypertensive Rats.","authors":"Di Xie, Yanghong Dong, Jinyu Chi, Wanlin Li, Chunnan Liu, Yang Xu, Yang Li, Jingzhi Wang, Jinfeng Wu, Rui Wang, Kelaier Yang, Xinhua Yin","doi":"10.1007/s12010-025-05265-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05265-6","url":null,"abstract":"Research indicates that patients with salt-sensitive (SS) hypertension experience higher morbidity and target organ damage than in patients with non-SS hypertension. Dysregulated macrophage activation has been implicated in SS hypertension development, with lysine acetylation playing a role in modulating macrophage function. However, the role of macrophage acetylation patterns in SS hypertension remains unclear. This study aimed to investigate how acetylation regulates macrophage function and its role in the pathogenesis of SS hypertension. We employed quantitative acetylation proteomics to characterize the acetylome of bone marrow-derived macrophages in Dahl SS hypertensive rats fed either a high-salt or a low-salt diet. We identified 94 hyperacetylated and 49 hypoacetylated sites on 79 and 45 proteins, respectively, in the high-salt group. Notably, acetylation levels increased at lysine 20 (K20) and K46 on histone H2B, at K56 on H3, and at K77 and K79 on H4c2. We also identified conserved acetylation motifs, analyzed their Gene Ontology terms and pathways, and explored the protein-protein interactions of these differentially acetylated proteins using bioinformatics analyses. Finally, we validated the altered acetylation of H2, H3, H4, and several metabolic proteins using immunoprecipitation and western blotting. Overall, these findings offer insights into the role of lysine acetylation in macrophages from SS hypertensive rats, revealing potential therapeutic targets.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0