Applied Biochemistry and Biotechnology最新文献

{"title":"Alkaline Proteases from Rose Snapper (Lutjanus guttatus): Evaluation of Their Stability to Chemical Denaturants and Potential Application to Hydrolyze Seafood Waste Proteins.","authors":"Gissel Daniela Rios-Herrera, Gabriela Miranda Pedroza-Toledo, Idalia Osuna-Ruiz, Emmanuel Martínez-Montaño, Jorge Manuel Sandoval-Gallardo, Jesús Aarón Salazar-Leyva","doi":"10.1007/s12010-024-05126-8","DOIUrl":"https://doi.org/10.1007/s12010-024-05126-8","url":null,"abstract":"<p><p>Large quantities of by-products are generated after processing of rose snapper (Lutjanus guttatus), such as viscera, head, tail, skin, and bones, which are considered a potential source of valuable molecules. Therefore, the aim of the present study was the biochemical characterization of alkaline proteases isolated from the intestines of L. guttatus and the evaluation of their stability against different chemical denaturants (salts, surfactants/reducing agents, organic solvents, and commercial detergent formulations). In addition, the efficiency to hydrolyze proteins from rose snapper wastes (head, tail, skin, and muscle trimmings) by Alcalase® and alkaline protease extract (APE) isolated from Lutjanus guttatus intestine was evaluated. The APE exhibited a maximum activity at pH 12 and 45 °C and high stability at pH and temperature ranges from 9 to 12 and 10 to 40 °C, respectively. Assays with specific protease inhibitors indicated that trypsin and chymotrypsin are the main types of proteases in APE. An 80% of the proteolytic activity was retained in the presence of 25% NaCl and was stable in the presence of the reducing agent DTT; however, it lost around 70% of proteolytic activity in the presence of 2-mercaptoethanol. The enzymatic activity of APE was maintained above 60% in methanol, ethanol, and propanol as well as in liquid commercial detergents. Alkaline proteases from rose snapper exhibited higher hydrolytic efficiency, compared to the microbial enzyme Alcalase when protein from L. guttatus wastes were hydrolyzed. According to these results, the integral exploitation of rose snapper could be reached by proper usage of its by-products, creating a baseline to promote circular economy.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric Analysis and Network Visualization of Nanozymes for Microbial Theranostics in the Last Decade.","authors":"Hamza Ettadili, Caner Vural","doi":"10.1007/s12010-024-05120-0","DOIUrl":"https://doi.org/10.1007/s12010-024-05120-0","url":null,"abstract":"<p><p>Nanozymes are a class of nanomaterials that are capable of mimicking the activities of natural enzymes. They are currently receiving considerable attention due to their advantageous properties. The objective of this study is to provide a comprehensive analysis of the advancements and trends in nanozymes for microbial theranostics research over the past decade through a detailed bibliometric approach. For this purpose, an effective search query was formulated, and relevant publications from 2013 to 2023 were collected from the Web of Science Core Collection database. Subsequently, the following softwares were employed for analysis: VOSviewer, the Bibliometrix R package, and GraphPad Prism 8.0.2. The findings revealed a statistically significant positive correlation (r = 0.993; p < 0.0001) between publications and citations, in addition to an important growth rate of scientific output of approximately 28.90%. China, India, and the USA were the most productive countries, whereas progress in low- and middle-income countries remained constrained. The Chinese Academy of Sciences was the most productive institution, and remarkably almost the top 10 productive authors were from China. Regarding keywords analysis, current research hotspots are primarily concentrated on the application of nanozymes in anti-biofilm-related research, antibacterial activity and therapy, the development of biosensors for microbial detection and control, and the advancement of wound disinfection and therapy.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of Rice Zinc Content Using Green Synthesized ZnO-NPs by Foliar and Nano-Priming Applications.","authors":"Nayab Ahmad, Sidra Ahmad, Afife Busra Ugur Kaplan, Sezai Ercisli, Mian Afaq Ahmad, Adewale Allen Sokan-Adeaga, Ghulam Murtaza, Humaira Rizwana, Saeedah Musaed Almutairi, Rashid Iqbal","doi":"10.1007/s12010-024-05127-7","DOIUrl":"https://doi.org/10.1007/s12010-024-05127-7","url":null,"abstract":"<p><p>Zinc oxide nanoparticles (ZnO-NPs) can enhance zinc bioavailability in plants, improving crop nutritional quality and addressing global zinc deficiency. This study aimed to investigate the effects of zinc oxide nanoparticles (ZnO-NPs), obtained by a green synthesis method, on the growth, yield parameters, and zinc content of rice plants. In the study, two different application strategies of ZnO-NPs on rice plants were evaluated, i.e., foliar spray and seed priming. To compare the effects of these application strategies, rice plants were treated with ZnO-NPs at two different concentrations, 25 mg/L and 50 mg/L. Effects of ZnO-NPs on plant growth (shoot and root length, fresh and dry mass), chlorophyll and carotenoid content, grain yield and zinc content were investigated. The results showed that both ZnO-NP application methods increased rice growth and yield, especially the combined method (seed priming + foliar spray) provided the highest efficiency. It was observed that seed zinc content was increased up to 10% by seed priming method and foliar spray application increased the zinc content up to 23% while the combination of seed priming and foliar spray increased zinc content up to 122%. In general, applications at 50 mg/L concentration increased more than 25 mg/L. These results indicate that ZnO-NPs can be an effective tool for zinc deficiency management in agricultural practices and can improve the yield and nutritional content of staple foods such as rice.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mining, Identification, and Fermentation Optimization of Chitin Deacetylase from a Novel Strain Enterobacter sp. ZCDA27.","authors":"Xi Qiao, Mengna Jiang, Enze Zhu, Yiwen Gu, Zhuoran Chen, Xin Ju, Liangzhi Li, Xia Zhong, Zhi Chen","doi":"10.1007/s12010-024-05124-w","DOIUrl":"https://doi.org/10.1007/s12010-024-05124-w","url":null,"abstract":"<p><p>Chitin, a natural organic compound with content slightly lower than cellulose, is also known for chitosan, a substance derived from chitin through deacetylation. In this experiment, preliminary screening was conducted using the plate discoloration circle method, leading to the selection of a high-yield CDA-producing strain from 28 candidates through rescreening. Morphological characteristics and 16S rDNA sequence analysis revealed 99.93% homology with Enterobacter sichuanensis strain N24, thus naming this strain Enterobacter strain ZCDA27. Initial fermentation of the strain yielded CDA activity of 9.29 U/mL. Single-factor optimization was then performed, followed by a PB test to screen for significant factors affecting enzyme production. The response surface method was used to further optimize the fermentation conditions. The optimal fermentation conditions for the carbon source, nitrogen source, metal ion, fermentation temperature, time, liquid volume, and initial pH were explored. Significant factors affecting enzyme production, including MgSO₄, initial medium pH, and fructose levels, were identified using the PB test. Finally, the fermentation conditions of ZCDA27 were optimized using the Box-Behnken design combined with RSM, which comprised fructose at 1.020%, magnesium sulfate at 0.016%, and peptone at 1%. The fermentation conditions included a temperature of 37, initial pH of 7.1, rotation speed of 140 × g, fermentation time of 28 h, inoculation amount of 2%, and liquid volume of 40%. Under these conditions, the enzyme activity of ZCDA27 reached 14.52 U/mL, a 1.6-fold increase from the pre-optimization levels. In summary, this study provides an experimental foundation for further development and application of Enterobacter spp. ZCDA27 CDA.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment of Vitamin A and Vitamin E in Sweet Corn Kernels Through Genomics-Assisted Introgression of Mutant Version of crtRB1 and vte4 Genes.","authors":"Hema S Chauhan, Rajkumar U Zunjare, Tuhin Rashmi, Vignesh Muthusamy, Abhijit K Das, Subhra J Mishra, Nisrita Gain, Brijesh K Mehta, Ashok K Singh, Hari S Gupta, Firoz Hossain","doi":"10.1007/s12010-024-05104-0","DOIUrl":"https://doi.org/10.1007/s12010-024-05104-0","url":null,"abstract":"<p><p>Recessive shrunken2 (sh2)-based sweet corn is preferred worldwide as it possesses higher sugar and extended shelf life. However, traditional sh2-based sweet corn is poor in vitamin A and vitamin E. Here, parental lines of two sh2-based sweet corn hybrids, viz. PSSC-2 and ASKH-2, were targeted for introgression of β-carotene hydroxylase 1 (crtRB1) and γ-tocopherol methyltransferase (vte4) genes through marker-assisted backcross breeding. Seeds with sh2sh2sh2 genotype in the endosperm were selected based on the shrunken phenotype in BC₁F₁, BC₂F₁ and BC₂F₂ generations. Gene-based markers, viz. 3'-TE-InDel and 118-InDel specific for crtRB1 and vte4, respectively, were successfully utilized for foreground selection in BC₁F₁, BC₂F₁ and BC₂F₂. Reconstituted hybrids showed high provitamin A (proA: 19.52 ± 0.52 µg/g) with a maximum of 7.8-fold increase over original hybrids (ASKH-2 and PSSC-2: 3.33 ± 0.28 µg/g). High α-tocopherol (20.75 ± 0.44 µg/g) and α/γ-tocopherol ratio (0.55 ± 0.02) with an average enhancement of 2.3- and 1.7-fold, respectively, was recorded among reconstituted hybrids over original versions (α-tocopherol: 9.21 ± 0.33 µg/g, α/γ-tocopherol ratio: 0.31 ± 0.01). The average yield of reconstituted hybrids (11.40 ± 0.22 t/ha) was at par with the original sweetcorn hybrids (11.60 ± 0.20 t/ha). This is the first report of stacking sh2, crtRB1 and vte4 genes to improve nutritional quality in sweet corn. These biofortified sweet corn hybrids hold immense significance to alleviate micronutrient malnutrition.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Analysis, Isolation, and Characterization of Gentiopicroside from Gentiana kurroo and Cytotoxicity of Biosynthesized Silver Nanoparticles Against HeLa Cells.","authors":"Upasana Sharma, Tikkam Singh, Veena Agrawal","doi":"10.1007/s12010-024-05114-y","DOIUrl":"https://doi.org/10.1007/s12010-024-05114-y","url":null,"abstract":"<p><p>Gentiana kurroo Royle, a critically endangered Himalayan herb, is valued in treating leucoderma, syphilis, bronchial asthma, hepatitis, etc. The current investigation performed quantitative and qualitative phytochemical analysis of G. kurroo root extracts prepared in chloroform, methanol, and ethyl acetate. The phenolic and flavonoid contents were the highest in methanol and chloroform extract, respectively. Several pharmacologically important compounds were identified through gas chromatography-mass spectrometry. Antioxidant analysis revealed methanolic extract to be the most efficient scavenger of 2,2-diphenyl-1-picrylhydrazyl (IC₅₀ = 114 µg mL^-1), hydrogen peroxide (IC₅₀ = 109.9 µg mL^-1), and superoxide (IC₅₀ = 74.63 µg mL^-1) radicals. Gentiopicroside was isolated from the methanolic root extract through silica-gel column-chromatography, and the characterization of concentrated fractions was achieved employing various analytical techniques. Pertaining to silver nanoparticle (GkAgNPs) synthesis, different physicochemical parameters were optimized and it was observed that root extract treated with silver-nitrate (0.5 mM) at 60 °C and incubated in dark for at least 120 min after initial color change, yielded GkAgNPs optimally. GkAgNPs were anisotropic and polydisperse and exhibited characteristic surface plasmon resonance (424 nm), crystalline face-centered cubic geometry, size (50-300 nm), and zeta-potential (- 16.3 mV). FT-IR spectra indicated the involvement of phenols and flavonoids in AgNPs synthesis. GkAgNPs were evidenced as strongly cytotoxic (IC₅₀ = 1.964 µg mL^-1) against HeLa cells and also showed deformed cellular morphology, a significant reduction in viable cell counts and colony-forming efficiency (4.08%). The findings suggest potential applications in drug development for treating serious human diseases. To the best of our knowledge, this study represents the first report on the isolation of gentiopicroside, the bio-fabrication of GkAgNPs using G.kurroo root extract, and their strong bioefficacy against HeLa cells.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Review on Preparation of Silver Nanoparticles from a Bacteriocin for the Natural Preservation of Food Products.","authors":"Ashmitha Kalairaj, Swethashree Rajendran, R Karthikeyan, Rames C Panda, T Senthilvelan","doi":"10.1007/s12010-024-05122-y","DOIUrl":"https://doi.org/10.1007/s12010-024-05122-y","url":null,"abstract":"<p><p>Food preservation aims to maintain safe and nutritious food for extended periods by inhibiting microbial growth that causes spoilage and poses health risks. Traditional chemical preservatives like sodium sulfite, sodium nitrite, sodium benzoate, tBHQ and BHA have raised concerns due to potential carcinogenicity, genotoxicity and allergies with long-term consumption. As a natural alternative, bacteriocins have emerged for food preservation. These ribosomally synthesised antimicrobial peptides are produced by various microorganisms, including bacteria, fungi and yeast, typically during their stationary growth phase. Bacteriocins are categorised into four classes based on structure and function, with molecular weights averaging between 30 and 80 kDa. They exhibit antimicrobial activity against a range of bacteria, mediating complex interactions between bacterial species and enhancing competitiveness and survival of producer strains. Both gram-positive and gram-negative bacteria produce bacteriocins. Recent advancements have identified and optimized bacteriocins for applications in food technology, extending shelf life, managing foodborne illnesses and contributing to public health preservation. Their eco-friendly nature and safety profile make bacteriocins promising for future food preservation strategies without detrimental effects on humans or animals. The current review has mainly focused on the preservation of food products using bacteriocin.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Study on Mechanical Properties of Cured Sand Combined with Plant-Based Bio-cement (PBBC) and Organic Materials.","authors":"Xiao Fu, Wan-Jun Ye, Gang Yuan, Xue-Li Zhang, Rui-Yuan Niu","doi":"10.1007/s12010-024-05131-x","DOIUrl":"https://doi.org/10.1007/s12010-024-05131-x","url":null,"abstract":"<p><p>Bio-cement is a green and energy-saving building material, which has received wide attention in the field of ecological environment and geotechnical engineering in recent years. The aim of this study is to investigate the improvement effect of plant-based bio-cement (PBBC) in synergistic treatment of sand with organic materials, to highlight the effective use of tap water in PBBC, and to analyze the crack evolution pattern during the damage of specimens by using image processing techniques. The results showed that tap water can be used as a solvent for PBBC instead of deionized water. The characteristic trend of urease solutions prepared at different temperature environments was obvious, and the activity value of urease solution with low concentration is positively correlated with the ambient temperature, although the activity value is not high, it is not easy to inactivate. The incorporation of organic materials increased the peak stress up to 1809.30 kPa compared to the specimens modified only by PBBC. The damage of the specimens under uniaxial compression consisted of four stages: compaction, elastic deformation, pre-peak brittle damage and post-peak macroscopic damage. The corresponding crack evolution is the interpenetration of small-sized cracks into large-sized main cracks. The large-sized main cracks transform into penetration cracks before damage, and the small-sized cracks are distributed around the penetration cracks. The crack evolution parameters obtained by MATLAB processing are positively correlated with the strain.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS Analysis of Polysaccharides from an Intergeneric Hybrid of Pleurotus florida and Cordyceps militaris: A Comparative Study.","authors":"Varsha Meshram, Prachi Thakur, Shailesh Kumar Jadhav, Nagendra Kumar Chandrawanshi","doi":"10.1007/s12010-024-05121-z","DOIUrl":"https://doi.org/10.1007/s12010-024-05121-z","url":null,"abstract":"<p><p>Edible and medicinal mushrooms are valuable sources of polysaccharides, known for their dual roles as immunostimulants and immunosuppressants. This study aimed to enhance polysaccharide content by fusing two mushroom species, P. florida and C. militaris, while exploring their antioxidant and antibacterial potential. These mushrooms have diverse health benefits, including lowering high cholesterol, providing anti-inflammatory effects, supporting diabetes management, aiding in cancer treatment, and enhancing the efficacy of COVID-19 vaccines. Successful hyphal fusion was achieved, and optimal culture conditions were determined using response surface methodology. The hybrids exhibited superior growth compared to the parental strains. Hyphal fusion improved several attributes, resulting in diverse hybrids with increased biomass and metabolite production. FTIR analysis confirmed the presence of exopolysaccharides, with concentrations measured at 28.4 g/L (P1), 31.50 g/L (CD), and 36.74 g/L (F3). GC-MS analysis identified various bioactive metabolites, including a higher concentration of dimethyl palmitamine in the hybrid, a novel compound, butanenitrile, 2-(methoxymethoxy), which was not found in the parental strains. These compounds are likely responsible for the enhanced antimicrobial and antioxidant activities.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer-Based Detection of Foot-and-Mouth Disease Virus Using Single-Stranded DNA Probe.","authors":"Nor Aina Nordin, Samson Soon, Jamaliah B Senawi, Zurin Azlin M Jinin, Siti Suri Arshad, Abdul Rahaman Yasmin, Farah Asilah Azri","doi":"10.1007/s12010-024-05093-0","DOIUrl":"https://doi.org/10.1007/s12010-024-05093-0","url":null,"abstract":"<p><p>Foot-and-mouth disease (FMD) is known for its highly contagious properties among cloven-hoofed animals resulting in significant morbidity rates. Incursions of this disease have caused significant losses in affected countries in Southeast Asia and Africa, even within EU countries which resulted in significant financial losses. This study is aimed at addressing existing limitations by creating a diagnostic method using aptamer-based assay. Three DNA aptamers were engineered to target the VP2 region of the FMD viral capsid protein. Since VP2 demonstrates a highly conserved amino acid sequence across serotypes, the specifically designed aptamers can detect different serotypes of the virus. Aptamers were evaluated against VP2 capsid protein, which was synthesized based on sequences from serotypes A, O, and Asia 1 of the FMD virus. After the recombinant VP2 capsid protein was developed, expressed, and refined, it was applied using enzyme-linked aptamer sorbent assay (ELASA) to determine aptamers' binding capability. A similar test was further conducted with purified FMD virus from serotype A and serotype O. The ELASA results displayed a notable sensitivity in identifying the FMDV. Under optimized conditions, the aptamers have LOD as low as 0.11 ng/mL with LOQ as low as 0.34 ng/mL. The binding strength analyzed using the equilibrium dissociation constant (Kd) showed strong binding affinity at 3.092 ± 0.05 nM. Based on these findings, the method shows significant potential with high sensitivity and specificity for FMD virus detection assay.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}