Applied Biochemistry and Biotechnology最新文献

{"title":"Quercetin Derivatives from Bidens pilosa Suppressed Cell Proliferation via Inhibition of RSK2 Kinase and Aldose Reductase Enzymes: UPLC-MS/MS, GC–MS, In Vitro, and Computational Studies","authors":"Doaa S. Ali,&nbsp;Alaadin E. El-Haddad,&nbsp;Hussein S. Mohamed,&nbsp;Ashraf A. El-Bassuony,&nbsp;Momtaz M. Hegab,&nbsp;Gehad AbdElgayed,&nbsp;Hossam Ebaid,&nbsp;Shimaa A. Ahmed,&nbsp;Emadeldin M. Kamel","doi":"10.1007/s12010-024-05134-8","DOIUrl":"10.1007/s12010-024-05134-8","url":null,"abstract":"<div><p>Traditionally, <i>Bidens</i> <i>pilosa</i> L. is an edible herb utilized for various ailments. The study accomplished a complete analysis of <i>B. pilosa</i> extract including UPLC/T-TOF–MS/MS, GC–MS, and in vitro antiproliferative activity, in addition to molecular docking on kinase and aldose reductase enzymes. From GC–MS analysis, the percentage of identified unsaturated fatty acids (FAs) (11.38%) was greater than saturated FAs (8.69%), while the sterols percent (39.92%) was higher than the hydrocarbons percent (6.6%). Oleic and palmitic acids are the major FAs (9.48% and 6.14%, respectively). Phytochemical profile uncovered the presence of quercetin, kaempferol, myricetin, and isorhamnetin aglycones and/or glycoside derivatives alongside apigenin, acacetin, and luteolin derivatives. <i>B. pilosa</i> extract suppressed cell proliferation in a concentration-dependent manner against SNB-19 and SK-MEL-5 cell lines (IC₅₀ 1.66 ± 0.06 and 4.04 ± 0.14 mg/mL, respectively). These potentials aligned with the molecular docking results on aldose reductase and kinase enzymes with promising binding affinities (− 5.3 to − 8.89 kcal mol⁻¹). <i>B. pilosa</i> metabolites were found as kinases and aldose reductase inhibitors, which rationalize their antiproliferative activity. Unfortunately, toxicity assessments were not performed to assess the safety of <i>B. pilosa</i> extract. Assessment of the therapeutic efficiency via in vivo and clinical studies is required.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2474 - 2492"},"PeriodicalIF":3.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12010-024-05134-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation and Characterization of Eco-Technological Synthesis of Spherical TiO2 Nanoparticles from Thalassia hemprichi and Analysis of Biomedical Properties","authors":"Velmani Sundar,&nbsp;Silambarasan Tamil Selvan,&nbsp;Arularasu M V,&nbsp;Maruthupandian Arumugam,&nbsp;Santhosh Chinnaraj","doi":"10.1007/s12010-024-05143-7","DOIUrl":"10.1007/s12010-024-05143-7","url":null,"abstract":"<div><p>In this present investigation, plant-mediated synthesis of titanium oxide (TiO₂) nanoparticles was synthesized from seagrass (<i>Thalassia hemprichi</i>) using the hot plate combustion method (HPCM). Synthesized TiO₂ nanoparticles optical, functional, structural, and morphology properties were analyzed by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). SEM analysis confirmed the spherical shape of the TiO₂ nanoparticles were observed in various sizes, viz., 50 nm and 78 nm. The XRD analysis revealed that TiO₂ nanoparticles have a body-centred cubic structure without a secondary phase. Green synthesized TiO₂ nanoparticle applications were studied against the antimicrobial, antioxidant, anticancer, and photocatalytic activity. The pathogenic bacterial strains, including <i>Staphylococcus epidermidis</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumonia</i>, and <i>Pseudomonas aeruginosa</i>, were tested against TiO₂ nanoparticles; the maximum level of activity was seen at a concentration of 50 µg/mL. The antioxidant assays were performed against TiO₂ nanoparticles, and inhibitory concentration values (IC₅₀) were 40.28 μg/mL of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 52.04 µg/mL of the acrylamide tertiary butyl sulfonic acid (ATBS) assay, and 16.91 µg/mL of the metal chelating assay. The anticancer activity was analyzed against MCF-7 cancer cells using TiO₂ nanoparticles, and the IC₅₀ value showed 64.14 µg/mL concentration. An eco-friendly and convenient method was formulated for the production of titanium oxide nanoparticles utilizing seagrass extract. The potential employment of TiO₂ involves water treatment, biomedicine, biosensors, and nanotechnology.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2457 - 2473"},"PeriodicalIF":3.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and Genomic Potential of Burkholderia contaminans PB_AQ24 Isolate for Boosting the Growth of Bamboo Seedlings in Heavy Metal Contaminated Soils","authors":"Poonam Bhanse,&nbsp;Lal Singh,&nbsp;Asifa Qureshi","doi":"10.1007/s12010-024-05156-2","DOIUrl":"10.1007/s12010-024-05156-2","url":null,"abstract":"<div><p>The present study investigated the genomic and functional potential of <i>Burkholderia contaminans</i> PB_AQ24, a bacterial strain isolated from the municipal solid waste dumpsite, for boosting the growth of <i>Dendrocalamus strictus</i> (Male bamboo) seedlings. The isolated strain exhibited high potency for metal solubilization and ACC (1-Aminocyclopropane-1-carboxylate) deaminase activity. Its genome harbored diverse genes responsible for nitrogen and phosphorus utilization (<i>trpABCDES</i>, <i>iaaH</i>, <i>acdS</i>, <i>pstABCS</i>, <i>phoAUD</i>, <i>pqqABCDE</i>, <i>kdpABC</i>, <i>gln</i>, and <i>nirBD</i>) and also an abundance of heavy metal tolerant genes (<i>ftsH</i>, <i>hptX</i>, <i>iscX</i>-<i>fdx</i>-<i>hscAB</i>-<i>iscAUR</i>, <i>mgtA</i>, <i>corA</i>, and <i>copC</i>). Seeds priming experiments carried out in heavy metal contaminated soils (such as waste dumpsite soil (WDS), fly ash dumpsite soil (FAS) and natural garden soil (NGS control)) augmented with <i>Burkholderia contaminans</i> sp. PB_AQ24 showed 85% sustenance of seedlings in WDS and 80% in FAS. The study thus highlighted the potential features in isolated bacterial strain <i>Burkholderia</i> sp. PB_AQ24 (NCBI accession no. JAQOUG000000000), which could boost the growth of bamboo seedlings in heavy metal contaminated soils and may be applied for restoration and rejuvenation of contaminated sites.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2437 - 2456"},"PeriodicalIF":3.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quinoline: A Novel Solution for Next-Generation Pesticides, Herbicides, and Fertilizers","authors":"Shahid Ullah Khan,&nbsp;Taufiq Nawaz,&nbsp;Osama Alam,&nbsp;Dilfaraz Khan,&nbsp;Shah Fahad,&nbsp;Shah Saud,&nbsp;Kun Lu","doi":"10.1007/s12010-024-05164-2","DOIUrl":"10.1007/s12010-024-05164-2","url":null,"abstract":"<div><p>Quinoline is a nitrogen-containing heterocycle compound widely used in the medical industry for its pharmacological properties, such as its antimalarial, antimicrobial, antiparasitic, anti-inflammatory, and anticancer activities. Beyond its medical significance, quinoline shows promising applications in agriculture as a safe and effective pesticide, herbicide, and fertilizer. This review explores the evolution of quinoline research, beginning with its history and synthesis and transitioning to its biological activities and their relevance in agriculture. It then highlights the potential applications of quinoline in modern agriculture, such as pesticides, herbicides, and fertilizers, for increasing crop yields and resilience while reducing crop waste. Moreover, it discusses formulation strategies that can enhance the efficacy of quinoline. Finally, the review addresses potential challenges, such as toxicity and environmental impact, underscoring the need for further research to harness quinoline’s full potential in sustainable agriculture.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2097 - 2119"},"PeriodicalIF":3.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Benzo[d]thiazol-2-Amine Derivatives, Synthesis, and Molecular Docking Insights Potential Anticancer Agents Targeting HER Enzyme and DNA","authors":"Ammar M. K. AL-Azzawi,&nbsp;Ekhlas Abdallah Hassan","doi":"10.1007/s12010-024-05149-1","DOIUrl":"10.1007/s12010-024-05149-1","url":null,"abstract":"<div><p>The synthesis and characterization of benzo[d]thiazol-2-amine derivatives, which were prepared by reacting benzothiazole with para-aminobenzophenone in ethanol, supplemented with glacial acetic acid. Subsequently, compound (2) was synthesized from compound (1) using NaNO₂, H₃PO₄, and HNO₃ in a water-based solvent, resulting in 2-hydroxy-1-naphthaldehyde. Another derivative, compound (3), was synthesized by reacting compound (1) with vanillin under similar conditions. Structural characterization involved IR spectroscopy and melting point determination, while molecular properties were estimated to assess drug-like characteristics. The main point of this study is to synthesize and research drug-like characteristics, biological activities, and docking studies. Molecular docking studies (MDS) were conducted using AutoDock Vina to evaluate the binding affinity of compounds 1, 2, and 3 with the enzyme Human Epidermal growth factor receptor (HER). The docking simulations aimed to elucidate drug-DNA interactions, focusing on hydrogen bonding, hydrophobic interactions, and binding energies. The compounds’ conformations were analyzed to identify their potential binding modes within the DNA groove. Compounds 2 and 3 exhibited higher binding affinities to the HER enzyme compared to compound 1, with compound 2 showing the highest affinity docking scores of − 10.4, − 9.9, and − 9.8 kcal/mol for the top three poses. These results suggest that compounds 2 and 3 could potentially interact more effectively with the enzyme and DNA, attributed to their structural features and interaction profiles. Synthesized and characterized benzo[d]thiazol-2-amine derivatives and evaluated their biological activities against gram-positive and gram-negative bacteria. The compounds demonstrated diverse biological activities, likely due to the various functional groups within their 4- to 5-ring structures. Molecular docking studies indicated that compounds 2 and 3 have promising potential as cancer therapy candidates, showing strong binding affinities to the HER enzyme and effective interactions with DNA.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2383 - 2396"},"PeriodicalIF":3.1,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly Synthesis of Iron Oxide Nanoparticles Using Parietaria alsinifolia Extracts and Evaluation of Biological Applications","authors":"Zakir Ullah,&nbsp;Javed Iqbal,&nbsp;Banzeer Ahsan Abbasi,&nbsp;Farhat Gul,&nbsp;Sarfaraz Ali,&nbsp;Sobia Kanwal,&nbsp;Reem M. Aljowaie,&nbsp;Ghulam Murtaza,&nbsp;Rashid Iqbal,&nbsp;Tariq Mahmood","doi":"10.1007/s12010-024-05151-7","DOIUrl":"10.1007/s12010-024-05151-7","url":null,"abstract":"<div><p>The current research was conducted to synthesize <i>Parietaria alsinifolia-</i>mediated iron oxide nanoparticles (P.A@FeONPs) using the green and eco-friendly protocol. The biosynthesized P.A@FeONPs were characterized using various approaches like UVs, FTIR, SEM, EDX, and DLS. The mean crystallite size was calculated to be ~ 21.48 nm using the Debye–Scherrer equation. Further, various in vitro biological assays were performed to analyze the therapeutic potentials of FeONPs. 2,2-Diphenyl-1-picrylhydrazy (DPPH) antioxidant activity was performed to reveal the DPPH radical scavenging potential of P.A@FeONPs and was calculated as 72%. Similarly, the total reducing power was determined as 65.45 ± 1.77%. In addition, P.A@FeONPs exhibited a significant total antioxidant capacity of 87 ± 4.8%. Antibacterial and antifungal assays were performed using the disc diffusion method. Among the different bacterial strains accession (EFB-10–2023 M.B), <i>Rhodococcus jostii</i> has shown the highest zone of inhibition (23.9 mm at 1000 μg/mL), while <i>Escherichia coli</i> displayed a 22.65 mm zone of inhibition at (1000 μg/mL). Similarly, <i>Aspergillus niger</i> exhibited a substantial zone of inhibition (28.75 mm). A brine shrimp cytotoxicity assay revealed the cytotoxicity potential (LC₅₀ 244.92 μg/mL). P.A@FeONPs were also tested against red blood cells, HEK-293, and VERO cell lines (&lt; 200 μg/mL) to validate their biocompatibility. An alpha-amylase inhibition assay demonstrated 68.66% inhibition and substantial cytotoxicity against Hep-2 liver cancer cells (IC₅₀ 100 μg/mL). In conclusion, P.A@FeONPs have shown significant bioactivities. In the future, we recommend other biological and catalytic activities using different animal models to explore its potential further.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2328 - 2355"},"PeriodicalIF":3.1,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning, Heterologous Expression, and Biochemical Characterization of a Novel Glycoside Hydrolase 16 Family Enzyme for Biorefinery of Furcellaria lumbricalis","authors":"Limin Ning,&nbsp;Yanshang Wei,&nbsp;Zilong Guo","doi":"10.1007/s12010-024-05152-6","DOIUrl":"10.1007/s12010-024-05152-6","url":null,"abstract":"<div><p>Carrageenan has strong structural heterogeneity, resulting in the production of several hybridized forms in nature. Furcellaran is a typical hybrid type of carrageenan that includes both κ-carrageenan and β-carrageenan motifs in its structure. The discovery and characterization of a novel furcellaranase is of great significance for investigating and determining the structures of carrageenan. Herein, a new GH 16 enzyme CeFurA, with furcellaran and porphyran degrading activities, was cloned, and it included 350 amino acid residues and has a predicted theoretical molecular weight of 40.45 kDa. The enzyme displayed the highest biological activity (824.64 U/mg) on furcellaran at 35 °C and pH 9.0. Notably, CeFurA has excellent temperature stability throughout the wide 25 to 40 °C temperature range. It is useful and promising to efficient prepare hybrid bk-carrageenan oligosaccharides and elucidate the fine structure of the hybrid polysaccharide and oligosaccharides. TLC and ESI–MS indicate that CeFurA, as an endo-type enzyme, can specifically act on DA-Gβ1 → 4DA-G and DA-G4Sβ1 → 4DA-G4S glycosidic linkages within the furcellaran, producing disaccharides, tetrasaccharides, and hexasaccharides as the primary products. The CeFurA exhibited a sandwich-like structure according to structural modeling, which contains an embedded catalytic chamber formed by the β folded sheets placed in a reversing manner by acting on the internal DA-G4Sβ1 → 4DA-G4S glycosidic link. These exceptional properties make CeFurA a powerful tool for studying the heterogeneity of carrageenan structures and producing COS in the industry.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2312 - 2327"},"PeriodicalIF":3.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12010-024-05152-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WTAP Promotes Atherosclerosis by Inducing Macrophage Pyroptosis and M1 Polarization through Upregulating NLRP3","authors":"Xing Luo,&nbsp;Chaogui He,&nbsp;Bo Yang,&nbsp;Shuheng Yin,&nbsp;Ke Li","doi":"10.1007/s12010-024-05106-y","DOIUrl":"10.1007/s12010-024-05106-y","url":null,"abstract":"<div><p>The study was designed to investigate the impact of N6-methyladenosine (m6A) writer Wilms tumor 1-associated protein (WTAP) on the progression of atherosclerosis (AS) and to further elucidate its possible regulatory mechanism. The m6A levels and WTAP expressions were initially assessed through RIP, qRT-PCR, and western blotting. An in vitro model of AS was constructed by ox-LDL treatment in RAW264.7 cells. Next, the impact of WTAP on macrophage pyroptosis and M1 polarization was evaluated. The relationship between WTAP and NLRP3 was then investigated using m6A modification quantification and RIP-qPCR assay. To investigate the effect of WTAP on AS development in vivo, we created an <i>ApoE</i>^−/−mouse model of AS by feeding high-fat diet (HFD). Furthermore, the influence of WTAP on macrophage pyroptosis and M1 polarization through NLRP3 was explored by NLRP3 overexpression AAV injection. Here, we found that WTAP was significantly upregulated in peripheral blood mononuclear cells (PBMCs) from AS patients, accompanied by increased total m6A methylation levels. The silencing of WTAP suppressed macrophage pyroptosis and M1 polarization induced by ox-LDL and also ameliorated aortic root lesion damage in AS mice. Mechanistically, m6A modification mediated by WTAP enhanced <i>NLRP3</i> mRNA stabilization, thereby upregulating NLRP3 expression. Overexpression of NLRP3 was found to enhance macrophage pyroptosis and M1 polarization, contributing to the progression of AS. In conclusion, our findings suggest that WTAP knockdown mitigated AS progression by modulating NLRP3 in an m6A-dependent manner. Our study proposes that targeting WTAP could be a potential preventive and therapeutic strategy for AS patients.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2397 - 2416"},"PeriodicalIF":3.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Identification, and Fermentation Optimization of Phytase-Producing Bacteria and Their Effects on Soybean Seedlings","authors":"Limin Zhang,&nbsp;Ziwei Song,&nbsp;Jingyuan Guo,&nbsp;Wenjia Liu,&nbsp;Jie Li,&nbsp;Qingxin Meng,&nbsp;Jixian Mo","doi":"10.1007/s12010-024-05154-4","DOIUrl":"10.1007/s12010-024-05154-4","url":null,"abstract":"<div><p>Phosphorus in soil mostly exists in complex compounds such as phytic acid, which reduces the effectiveness of phosphorus and limits agricultural production. Phytase has the activity of hydrolyzing phytate into phosphate. The mineralization of phytate in soil by phytase secreted by microorganisms is an effective way to improve the utilization rate of phytate. This study isolated a high-yield phytase strain, identified as <i>Pseudomonas</i> by 16S rDNA and named <i>Pseudomonas</i> sp. S3-10. The fermentation medium composition and conditions were optimized using the single-factor method, Plackett–Burman design (PBD), and response surface methodology (RSM). The results showed that cane molasses, MgCl₂, and temperature significantly affected the fermentation biomass of the bacterium. The optimal fermentation conditions were cane molasses and MgCl₂ concentrations of 61.80 g/L and 5.94 g/L, respectively, at 34.4 °C. Compared with the unoptimized fermentation conditions, the maximum biomass increased by 160.17 ± 6.26% under the optimized fermentation conditions, reaching 9.13 ± 0.09 × 10⁹ CFU/mL. The pot experiment results showed that <i>Pseudomonas</i> sp. S3-10 has a significant promoting effect on soybean growth. The strain increased the fresh weight and length of soybean seedlings by 112.92 ± 28.41% and 74.02 ± 3.24%, respectively, and increased the phytase activity in the soil and available phosphorus concentration in the plant rhizosphere by 388.15 ± 24.24% and 365.05 ± 91.96%, respectively. This study provided a high-yield phytase strain and its optimal fermentation conditions. The bacterium has significant plant growth-promoting effects and can be used as a new type of biological fertilizer, which is of great significance for reducing phosphorus fertilizer usage, improving phosphorus utilization efficiency, and protecting the ecological environment in agricultural production.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2417 - 2436"},"PeriodicalIF":3.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Plant UDP-Glycosyltransferases for Betanin Production in Yeast","authors":"Christiane Glitz,&nbsp;Jane Dannow Dyekjær,&nbsp;Dovydas Vaitkus,&nbsp;Mahsa Babaei,&nbsp;Ditte Hededam Welner,&nbsp;Irina Borodina","doi":"10.1007/s12010-024-05100-4","DOIUrl":"10.1007/s12010-024-05100-4","url":null,"abstract":"<div><p>To cover the rising demand for natural food dyes, new sources and production methods are needed. Microbial fermentation of nature-identical colours, such as the red pigment betanin, has the potential to be a cost-efficient alternative to plant extraction. The last step of betanin production is catalysed by a UDP-glycosyltransferase (UGT). To find a high-performing UGT, we screened 27 UGTs from different plant species and tested their ability to produce betanin in vivo in <i>Saccharomyces cerevisiae</i>. We identified two new UGTs likely involved in the betanin synthesis in the plant they derive from: CqGT2 (UGT73A37) from <i>Chenopodium quinoa</i> and BgGT2 (UGT92X1) from <i>Bougainvillea glabra.</i> The betanin-producing UGTs were also tested in <i>Yarrowia lipolytica</i>, where CqGT2 was the best-performing glycosyltransferase for betanin production. While it has previously been shown that the UGTs can glycosylate either betanidin or cyclo-DOPA to ultimately form betanin, the molecular mechanism behind the preference for the acceptor molecule has not been elucidated. Therefore, we performed in silico structural analysis to characterise the betanin-producing UGTs further, particularly by looking into their binding mechanism. The docking model suggested that a smaller binding site found in some UGTs only allows glycosylation of cDOPA, while a wider binding site allows glycosylation of both cyclo-DOPA and betanidin.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"197 4","pages":"2356 - 2382"},"PeriodicalIF":3.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12010-024-05100-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}