Applied Biochemistry and Biotechnology最新文献

{"title":"Clinical implications and mechanism of complement C1q in polymyositis","authors":"Di Wu,&nbsp;Yan Cui,&nbsp;Yujia Cao,&nbsp;Yanjuan Wang,&nbsp;Jinhua Zhang,&nbsp;Yijing Guo,&nbsp;Baoyu Yuan","doi":"10.1007/s12010-023-04692-7","DOIUrl":"10.1007/s12010-023-04692-7","url":null,"abstract":"<div><p>Polymyositis (PM) is the most common autoimmune disease in neurology and among muscle disorders; it is of great significance to thoroughly understand the mechanism of PM to find new diagnosis and treatment methods. This research intends to elucidate the clinical implications and mechanisms of complement C1q in polymyositis (PM). One hundred fifteen PM patients (research group, RG) and 120 healthy subjects (control group, CG) who visited our hospital between March 2017 and March 2020 were selected. Peripheral blood C1q and creatine kinase (CK) levels of both groups were measured, and their correlations with clinical symptoms and prognostic recurrence of PM. Additionally, to further understand the mechanism of action of C1q in PM, we purchased BALB/c mice and grouped them as follows: control group with normal feeding, PM group with PM modeling, intervention group with PM modeling, and intraperitoneal injection of gC1qR monoclonal antibody 60.11, a C1q protein receptor. Inflammatory factors and muscle histopathology were detected in all groups of mice. Finally, rat macrophages (mø) were isolated, and changes in the biological behavior of mø were observed after silencing the expression of gC1qR. Serum C1q and CK were both higher in RG than in CG, with favorable diagnostic effects on PM (<i>P</i> &lt; 0.05). C1q and CK increased in symptomatic anti-ribonuclear protein antibody (RNP)–positive patients but decreased in anti Jo-1 antibody (Jo-1)– and anti-neutrophil cytoplasmic antibody (ANCA)–positive patients (<i>P</i> &lt; 0.05). PM mice were observed with elevated gC1qR, while model mice exhibited severe infiltration of inflammatory cells in muscle tissue, increased pro-IFs, and reduced anti-IFs, and the animals in the intervention group showed improved conditions (<i>P</i> &lt; 0.05). Finally, it was found that CD68, CD86 protein, and invasion capacity of gC1qR-sh-transfected cells decreased, while CD206 and CD163 increased (<i>P</i> &lt; 0.05). C1q is elevated in PM and is strongly linked to the pathological process of PM. Inhibition of gC1qR expression reduced inflammatory infiltration in PM mice.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 6","pages":"3088 - 3101"},"PeriodicalIF":3.1,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10124622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond Conventional Meat Preservation: Saddling the Control of Bacteriocin and Lactic Acid Bacteria for Clean Label and Functional Meat Products","authors":"Slim Smaoui,&nbsp;Noemí Echegaray,&nbsp;Manoj Kumar,&nbsp;Moufida Chaari,&nbsp;Teresa D’Amore,&nbsp;Mohammad Ali Shariati,&nbsp;Maksim Rebezov,&nbsp;Jose Manuel Lorenzo","doi":"10.1007/s12010-023-04680-x","DOIUrl":"10.1007/s12010-023-04680-x","url":null,"abstract":"<div><p>Advancements in food science and technology have paved the way for the development of natural antimicrobial compounds to ensure the safety and quality of meat and meat products. Among these compounds, bacteriocin produced by lactic acid bacteria has gained considerable scientific attention for its ability to preserve the healthy properties of meat while preventing spoilage. This natural preservative is seen as a pioneering tool and a potent alternative to chemical preservatives and heat treatment, which can have harmful effects on the nutritional and sensory qualities of meat. Bacteriocin produced by lactic acid bacteria can be used in various forms, including as starter/protective cultures for fermented meats, purified or partially purified forms, loaded in active films/coatings, or established in encapsulate systems. This review delves into the downstream purification schemes of LAB bacteriocin, the elucidation of their characteristics, and their modes of action. Additionally, the application of LAB bacteriocins in meat and meat products is examined in detail. Overall, the use of LAB bacteriocins holds immense potential to inspire innovation in the meat industry, reducing the dependence on harmful chemical additives and minimizing the adverse effects of heat treatment on nutritional and sensory qualities. This review provides a comprehensive understanding of the potential of bacteriocin produced by lactic acid bacteria as a natural and effective meat preservative.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 6","pages":"3604 - 3635"},"PeriodicalIF":3.1,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10416069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Rhodosporidium toruloides for Fatty Acids Production Using Lignocellulose Biomass","authors":"Sushant Sunder,&nbsp;Anshul Gupta,&nbsp;Rashmi Kataria,&nbsp;Rohit Ruhal","doi":"10.1007/s12010-023-04681-w","DOIUrl":"10.1007/s12010-023-04681-w","url":null,"abstract":"<div><p>Microbial lipids are ideal for developing liquid biofuels because of their sustainability and no dependence on food crops. Especially the bioprocess for microbial lipids may be made economical by using sustainable approaches, e.g., lignocellulose-based carbon sources. This demand led to a search for ideal microorganisms with the ability to utilize efficiently biomass into value-added products. <i>Rhodosporidium toruloides</i> species belongs to the family of oleaginous (OG) yeast, which aggregates up to 70% of its biomass to produce fatty acids which can be converted to a variety of biofuels. <i>R. toruloides</i> is extremely adaptable to different types of feedstocks. Among all feedstock, a lot of effort is going on to develop a bioprocess of fatty acid production from lignocellulose biomass. The lignocellulose biomass is pretreated using harsh conditions of acid, alkali, and other which leads to the generation of a variety of sugars and toxic compounds. Thus, so obtained lignocellulose hydrolysate may have conditions of different pH, variable carbon and nitrogen ratios, and other non-optimum conditions. Accordingly, a detailed investigation is required for molecular level metabolism of <i>R. toruloides</i> in response to the hydrolysate for producing desired biochemicals like fatty acids. The present review focuses on numerous elements and obstacles, including metabolism, biofuel production, cultivation parameters, and genetic alteration of mutants in extracting fatty acids from lignocellulosic materials utilizing <i>Rhodosporidium</i> spp. This review provides useful information on the research working to develop processes for lignocellulose biomass using oleaginous yeast.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 5","pages":"2881 - 2900"},"PeriodicalIF":3.1,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10116935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of ChREBP Gene Methylation with Pathological Characteristics of Type 2 Diabetes Mellitus","authors":"Xin Wang,&nbsp;Weizhong Huangfu,&nbsp;Feng Zhao","doi":"10.1007/s12010-023-04714-4","DOIUrl":"10.1007/s12010-023-04714-4","url":null,"abstract":"","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 6","pages":"3076 - 3087"},"PeriodicalIF":3.1,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10116932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Novel Genes and Pathways of Ovarian Cancer Using a Comprehensive Bioinformatic Framework","authors":"Hibba Rashid,&nbsp;Asad Ullah,&nbsp;Sajjad Ahmad,&nbsp;Salma Mohammed Aljahdali,&nbsp;Yasir Waheed,&nbsp;Bilal Shaker,&nbsp;Alhanouf I. Al-Harbi,&nbsp;Alhumaidi B. Alabbas,&nbsp;Safar M. Alqahtani,&nbsp;Maaged A. Akiel,&nbsp;Muhammad Irfan","doi":"10.1007/s12010-023-04702-8","DOIUrl":"10.1007/s12010-023-04702-8","url":null,"abstract":"<div><p>Ovarian cancer (OC) is a significant contributor to gynecological cancer-related deaths worldwide, with a high mortality rate. Despite several advances in understanding the pathogenesis of OC, the molecular mechanisms underlying its development and prognosis remain poorly understood. Therefore, the current research study aimed to identify hub genes involved in the pathogenesis of OC that could serve as selective diagnostic and therapeutic targets. To achieve this, the dataset GEO2R was used to retrieve differentially expressed genes. The study identified a total of five genes (CDKN1A, DKK1, CYP1B1, NTS, and GDF15) that were differentially expressed in OC. Subsequently, a network analysis was performed using the STRING database, followed by the construction of a network using Cytoscape. The network analyzer tool in Cytoscape predicted 276 upregulated and 269 downregulated genes. Furthermore, KEGG analysis was conducted to identify different pathways related to OC. Subsequently, survival analysis was performed to validate gene expression alterations and predict hub genes, using a <i>p</i>-value of 0.05 as a threshold. Four genes (CDKN1A, DKK1, CYP1B1, and NTS) were predicted as significant hub genes, while one gene (GDF15) was predicted as non-significant. The adjusted <i>P</i> values of said predicted genes are 2.85E − 07, 5.49E − 06, 4.28E − 07, 1.43E − 07, and 3.70E − 07 for CDKN1A, DKK1, NTS, GDF15, and CYP1B1 respectively; additionally 6.08, 5.76, 5.74, 5.01, and 4.9 LogFc values of the said genes were predicted in GEO data set. In a boxplot analysis, the expression of these genes was analyzed in normal and tumor cells. The study found that three genes were highly expressed in tumor cells, while two genes (CDKN1A and DKK1) were more elevated in normal cells. According to the boxplot analysis for CDKN1A, 50% of tumor cells ranged between approx 3.8 and 5, while 50% of normal cells ranged between approx 6.9 and 7.9, which is greater than tumor cells. This shows that in normal cells, the CYP1B1 has a high expression level according to the GEPIA boxplot; addtionally the boxplot for DKK1 indicated that 50% of tumor cells ranged between approx 0 and 0.5, which was less than that of normal cells which ranged between approx 0.3 and 0.9. It shows that DKK1 is highly expressed in normal genes. Overall, the current study provides novel insights into the molecular mechanisms underlying OC. The identified hub genes and drug candidate targets could potentially serve as alternative diagnostic and therapeutic options for OC patients. Further research is needed to investigate the clinical significance of these findings and develop effective interventions that can improve the prognosis of patients with OC.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 6","pages":"3056 - 3075"},"PeriodicalIF":3.1,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10116930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into Vitamin E with Combined Oral Contraceptive on INSR Gene in PCOS by Integrating In Silico and In Vivo Approaches","authors":"Rukaiah Fatma Begum,&nbsp;Sumithra Mohan","doi":"10.1007/s12010-023-04710-8","DOIUrl":"10.1007/s12010-023-04710-8","url":null,"abstract":"<div><p>Polycystic ovarian syndrome (PCOS) is a hormone disorder common among reproductive-aged women. This is associated with the symptoms like an irregular menstrual cycle, excess androgens, and polycystic ovary. Interestingly, vitamin E acts like the hormone progesterone and improves insulin sensitivity in PCOS. The study aims to evaluate the therapeutic effect of vitamin E in combination with combined oral contraceptive (COC) against PCOS by <i>in silico</i> and <i>in vivo</i> methods. The therapeutic effect of vitamin E (25 and 50mg/kg) in combination with COC (0.4mg/kg) was screened by the <i>in silico</i> method using Auto dock vina 4.2.6. Additionally, <i>in vivo</i> studies with a letrozole-induced PCOS model were performed in 30 female SD rats (<i>n</i> = 6 in each group) for 8 weeks with different doses of vitamin E. Furthermore, histopathological features and the insulin receptor (INSR) gene were scrutinized. An <i>in silico</i> study showed that drospirenone and vitamin E have an excellent affinity to bind to INSR and have higher binding energy (− 8.5 kcal/mol and − 8.7 kcal/mol, respectively). <i>In vivo</i> results showed a significant reduction in elevated testosterone levels compared to that of the PCOS group; follicle-stimulating hormone (FSH) and insulin levels also showed significant changes and reversed anti-oxidant levels in a dose-dependent manner. Ovarian histopathological changes were observed in different follicle counts in addition to the INSR gene, which showed changes in densitometry values. Supplementation of vitamin E combined with COC could be effective against PCOS, and clinical studies must be carried out further.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 6","pages":"2990 - 3009"},"PeriodicalIF":3.1,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Model Selection and Optimal Batch Design for Polyhydroxyalkanoate (PHA) Production by Cupriavidus necator","authors":"Pema Lhamo,&nbsp;Biswanath Mahanty","doi":"10.1007/s12010-023-04683-8","DOIUrl":"10.1007/s12010-023-04683-8","url":null,"abstract":"<div><p>Mathematical modelling of microbial polyhydroxyalkanoates (PHAs) production is essential to develop optimal bioprocess design. Though the use of mathematical models in PHA production has increased over the years, the selection of kinetics and model identification strategies from experimental data remains largely heuristic. In this study, PHA production from <i>Cupriavidus necator</i> utilizing sucrose and urea was modelled using a parametric discretization approach. Product formation kinetics and relevant parameters were established from urea-free experimental sets, followed by the selection of growth models from a batch containing both sucrose and urea. Logistic growth and Luedeking-Piret model for PHA production was selected based on regression coefficient (<i>R</i>²: 0.941), adjusted <i>R</i>² (0.930) and AICc values (−42.764). Model fitness was further assessed through cross-validation, confidence interval and sensitivity analysis of the parameters. Model-based optimal batch startup policy, incorporating multi-objective desirability, suggests an accumulation of 2.030 g l⁻¹ of PHA at the end of 120 h. The modelling framework applied in this study can be used not only to avoid over-parameterization and identifiability issues but can also be adopted to design optimal batch startup policies.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 5","pages":"2630 - 2651"},"PeriodicalIF":3.1,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smokeless Tobacco Harbors Bacteria Involved in Biofilm Formation as Well as Salt and Heavy Metal Tolerance Activity","authors":"Akanksha Vishwakarma,&nbsp;Digvijay Verma","doi":"10.1007/s12010-023-04689-2","DOIUrl":"10.1007/s12010-023-04689-2","url":null,"abstract":"<div><p>In our previous culture-independent study on smokeless tobacco products, we have observed a strong positive correlation between several bacteria and genes involved in nitrate/nitrite reduction, biofilm formation, and pro-inflammation. Therefore, the present investigation was carried out to analyze the inhabitant bacterial population of the Indian ST products for assessing the health-associated risk attributes using culture-dependent approach. Traditional cultivation approaches recovered several bacterial isolates from commercial ST products on different culture media. A high colony formation unit (CFU) count was observed that ranged from 173 × 10⁴ to 630.4 × 10⁵ per gram of ST products. Of the 74 randomly selected and distinct bacterial isolates, 17 isolates showed a significantly enhanced growth (<i>p-value</i> &lt; 0.05) in the presence of the aqueous tobacco extract. On biochemical characterization, these bacteria were identified as the member of <i>Bacillus, Enterobacter, Micrococcus, Providencia, Serratia, Pantoea, Proteus,</i> and <i>Pseudomonas</i>. Most of these bacteria also exhibited biofilm-forming activity, where eight bacterial isolates were identified for strong biofilm-forming action. 16S rRNA-based molecular characterization of these bacteria identified them as <i>Bacillus subtilis</i>, <i>Bacillus paralicheniformis</i>, <i>Enterobacter</i> sp.<i>, Serratia marcescens, Pantoea anthophila,</i> and <i>Enterobacter cloacae</i>. Moreover, these bacteria also exhibited the potential to withstand high salt and heavy metal concentrations. The findings demonstrate that Indian ST products are heavily populated with wide bacterial species exhibiting potential in biofilm formation, heavy metal resistance, and salt tolerance.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 6","pages":"3034 - 3055"},"PeriodicalIF":3.1,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS-Guided Antimicrobial Defense Responsive Secondary Metabolites from the Endophytic Fusarium solani Isolated from Tinospora cordifolia and Their Multifaceted Biological Properties","authors":"Fazilath Uzma,&nbsp;Srinivas Chowdappa,&nbsp;Arnab Roy,&nbsp;Shakeel Ahmed Adhoni,&nbsp;Daoud Ali,&nbsp;Kazunori Sasaki,&nbsp;Sudisha Jogaiah","doi":"10.1007/s12010-023-04669-6","DOIUrl":"10.1007/s12010-023-04669-6","url":null,"abstract":"<div><p>Medicinal plants are hosts to an infinite number of microorganisms, commonly referred to as endophytes which are rich in bioactive metabolites yielding favorable biological activities. The endophytes are known to have a profound impact on their host plant by promoting the accumulation of secondary metabolites which are beneficial to humankind. In the present study, the fungal endophyte, <i>Fusarium solani</i> (ABR4) from the medicinal plant <i>Tinospora cordifolia</i>, was assessed for its bioactive secondary metabolites employing fermentation on a solid rice medium. The crude ABR4 fungal extract was sequentially purified using the solvent extraction method and characterized using different spectroscopic and analytical techniques namely TLC, UV spectroscopic analysis, HRESI-MS, FTIR, and GC-MS analysis. The GC-MS analysis revealed the presence of pyridine, benzoic acid, 4-[(trimethylsilyl)oxy]-trimethylsilyl ester, hexadecanoic acid trimethylsilyl ester, and oleic acid trimethylsilyl ester. The cytotoxic ability of ABR4 was evaluated by MTT assay against lung cancer (A549) and breast cancer (MCF-7) cell lines. The compounds did not exhibit significant cytotoxicity against the tested cell lines. The endophytic ABR4 extract was evaluated for its antimicrobial potential against human pathogens (<i>S. aureus</i>, <i>B. cereus</i>, <i>E. coli</i>, <i>S. typhimurium</i>, <i>P. aeruginosa</i>, and <i>C. albicans</i>) by recording 47 to 54% inhibition. Taken together, the endophytic fungal strain ABR4 demonstrated a remarkable antimicrobial activity against the tested pathogens. Furthermore, the functional metabolites isolated from the endophytic strain ABR4 reveal its broader usage as antimicrobial agents for newer drug development in the pharmaceutical industry.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 6","pages":"3010 - 3033"},"PeriodicalIF":3.1,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10135610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light Controlled Nanobiohybrids for Modulating Chiral Alcohol Synthesis","authors":"Hang Yin,&nbsp;Shitong Cui,&nbsp;Yufei Cao,&nbsp;Jun Ge,&nbsp;Wenyong Lou","doi":"10.1007/s12010-023-04667-8","DOIUrl":"10.1007/s12010-023-04667-8","url":null,"abstract":"<div><p>The modulation of whole-cell activity presents a considerable challenge in biocatalysis. Conventional approaches to whole-cell catalysis, while having their strengths, often rely on complex and deliberate enzyme designs, which could result in difficulties in activity modulation and prolonged response times. Additionally, the activity of intracellular enzymes in whole-cell catalysis is influenced by temperature. To address these limitations, we introduced a relationally designed nanobiohybrid system that utilized light to modulate whole-cell catalysis for chiral alcohol production. By incorporating platinum nanoparticles onto <i>Rhodotorula</i> sp. cell surfaces, the nanobiohybrid capitalized on the photothermal properties of the nanoparticles to regulate the overall cell activity. When exposed to light, the Pt nanoparticles generate heat through the photothermal effect, consequently leading to an increase in the catalytic activity of the whole cells. This innovative approach facilitates control over whole-cell production and provides an efficient method for regulating biocatalytic processes. The findings of this study demonstrate the significant potential of switchable control strategies in biomanufacturing across a wide range of industries.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"196 6","pages":"2977 - 2989"},"PeriodicalIF":3.1,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10078677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}