Applied Biochemistry and Biotechnology最新文献

{"title":"Biochemical Mechanisms of Phosphodiesterase 6D Inhibition in K-Ras-Driven Cancers.","authors":"Mohammed Merae Alshahrani","doi":"10.1007/s12010-026-05643-8","DOIUrl":"https://doi.org/10.1007/s12010-026-05643-8","url":null,"abstract":"","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemopreventive Potential of Brucine-Gold Nanoparticles (BRU-AuNPs) in DMBA-induced Mammary Cancer via Modulation of PI3K/AKT/mTOR Pathway.","authors":"Saravanan Alamelu, Kamalesh Balakumar Venkatesan, Kalist Shagirtha, Manoj Kumar Srinivasan, Pugalendhi Pachaiappan","doi":"10.1007/s12010-026-05623-y","DOIUrl":"https://doi.org/10.1007/s12010-026-05623-y","url":null,"abstract":"<p><p>This study investigates the effects of brucine-gold nanoparticles (BRU-AuNPs) on rat mammary carcinogenesis by analysing biochemical, histological, and molecular interactions. Mammary cancer was induced in female Sprague-Dawley rats via subcutaneous injection of a chemical carcinogen (DMBA 25 mg/rat) near the mammary gland. Different concentrations of BRU-AuNPs (0.25, 0.5, and 1 mg/kg b.w) were orally administered for 112 days to assess the optimum dose. Parameters including body weight changes, tumor incidence, tumor volume, and tumor burden were analysed in control and experimental rats. Biochemical analyses include lipid peroxidation, antioxidant status, detoxification enzyme activities, and lipid profiles. Histopathological examinations of mammary tissues were performed to evaluate tissue and cellular integrity. The results revealed that BRU-AuNPs treatment to DMBA-injected rats significantly reduced incidence, tumor weight, and burden, lipid peroxidation, and phase I detoxification enzyme activities. Conversely, it improved body weight, phase II detoxification enzyme activities, and antioxidant status compared to DMBA-alone injected rats. Histopathological findings confirmed the protective effect of BRU-AuNPs against DMBA-induced tissue damage. Furthermore, molecular studies demonstrated that BRU-AuNPs modulate the PI3K/AKT/mTOR pathway by downregulating p-PI3K, p-AKT, and p-mTOR gene expression while upregulating PTEN and PKD1 levels. These molecular alterations were validated through immunohistochemistry and Western blot analyses. In conclusion, BRU-AuNPs exhibit significant anticancer effects by modulating oxidative stress and inhibiting the PI3K/AKT/mTOR pathway. These findings highlight their potential as a promising therapeutic strategy for breast cancer prevention.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Sustainable Copper Oxide Nanoparticles with Cordia Macleodii (Griff.) Hook.F. Thomson: Exploring Antimicrobial Activity and Antioxidant Defense","authors":"Umakant Pradhan,&nbsp;Purusottam Majhi,&nbsp;Sanjay Kumar Sahu,&nbsp;Anunay Toppo,&nbsp;Tanmay Kumar Ghorai,&nbsp;Awadhesh Kumar Shukla","doi":"10.1007/s12010-026-05625-w","DOIUrl":"10.1007/s12010-026-05625-w","url":null,"abstract":"<div>\u0000 \u0000 <p> <i>Cordia macleodii </i>(Griff.)Hook.F. Thomson leaves extract (CMLE) was used for the bio-fabrication of copper oxide nanoparticles (CM-CuO NPs). Spectrophotometer analysis revealed a surface plasmon resonance (SPR) peak at 280 nm and 336 nm, confirming the successful synthesis of stable copper oxide nanoparticles. Fourier transform infrared (FTIR) spectroscopy identified functional groups in leaves extracts that are essential for the formation of CM-CuO NPs. The Zeta PDI investigation showed a mean zeta potential of -5.1 ± 0.4 mV and a hydrodynamic diameter of 632.4 nm, with 22.9%. X-ray diffraction (XRD) analysis results shows the distinct 2𝜃 values between the 10–90° range with a crystalline size of 13.72 nm of the CM-CuO NPs. The Scanning electron microscopy (SEM) analysis provided a comprehensive structural view, revealing smaller, spherical CM-CuO NPs. The SEM-EDX signals from C, O, and Cu were indicated from the SEM-EDX. Whereas mapping analysis revealed element composition, Cu Lα, O Kα, and C Kα peaks were detected beyond those of copper and oxygen. Additionally, High-resolution Transmission electron microscopy (HR-TEM) investigation revealed a spherical form with an average particle size of 4.49 nm. The Antibacterial activity assessments of CM-CuO NPs against <i>Ralstonia solanacearum</i> indicated zones of inhibition of 19.33 ± 1.15 mm and 25.66 ± 1.52 mm at concentrations of 500 µg/mL and 1000 µg/mL, respectively. The reactive oxygen species (ROS) and cell viability tests of CM-CuO NPs against the tested bacterial strains confirmed a dose-dependent effect. The antifungal assessment of CM-CuO NPs against the fungi <i>Alternaria alternata</i>,<i> Aspergillus flavus</i>, and <i>Botrytis cinerea</i> showed inhibition of growth 55%, 75%, and 30%, respectively. The antioxidant free radical scavenging potential of CM-CuO NPs by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was assessed 14% and 38% by the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. Overall, in our study <i>C. macleodii</i> mediated CuO NPs shows effective antimicrobial activity. In future, studies focusing on the <i>in</i>-<i>vivo</i> biological applications, agricultural section, biomedical and environmental applications could establish these CM-CuO NPs as sustainable alternative to conventional chemical agents.</p>\u0000 </div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 5","pages":"4034 - 4058"},"PeriodicalIF":3.3,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen Peptide Infused Biphasic Gel Formulation for Burn Wound Management: Biophysical, Pharmacokinetic, and In Vivo Evaluation","authors":"Manjushree Kupendra,&nbsp;Vaishnavi Lokesh,&nbsp;Nethravathi Murdi,&nbsp;Johnson Furtado,&nbsp;Manjula Dotherabandi,&nbsp;Arpi Dey,&nbsp;Aneesa Fasim","doi":"10.1007/s12010-026-05626-9","DOIUrl":"10.1007/s12010-026-05626-9","url":null,"abstract":"<div><p>Although herbal and bioactive wound dressings are known to promote healing, integrated dressings combining both agents for burn wounds have not been reported. The present study describes the development of a novel herbal biphasic gel (bigel) uniquely combining fish-derived bioactive collagen peptides with phytochemical rich <i>Aloe vera</i> and virgin coconut oil addressing critical limitations of conventional hydrogels and organogels. Among the five formulations, F4 (80:20) hydrogel: organogel (v/v) demonstrated optimal pH, stability and spreadability. It also demonstrated sustained collagen peptide release (97.7%, <i>p</i> &lt; 0.0001) over 18 h following diffusion-controlled kinetics.<i> In vitro</i> studies confirmed its exceptional biocompatibility, demonstrating 100% cell viability, minimal hemolysis (4–6%), excellent antioxidant activity and significant cell migration (91.39% ± 1.95%, <i>p</i> &lt; 0.0001) at 1 mg/ml within 12 h. <i>In vivo</i> studies on male albino Wistar rats with deep partial-thickness burns showed substantial wound healing, with F4-treated wounds reducing to 0.3 cm (<i>p</i> &lt; 0.0001) by day 30, compared to 0.8 cm (<i>p</i> &lt; 0.0001) with Silverex^® (positive control) and 1.5 cm in untreated control. Histopathological analysis further supported these findings, revealing enhanced skin regeneration characterized by extensive collagen deposition, new blood vessel formation, and hair follicle development. These findings establish F4 bigel as a paradigm shift towards cost-effective, sustainable, and effective topical therapy for burn wound healing.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 5","pages":"4059 - 4081"},"PeriodicalIF":3.3,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Production of Bioethanol and Monascus Pigments: a Strategy of Trace Element Supplementation for Monascus purpureus Fermentation","authors":"Jianqi Han,&nbsp;Xinji Li,&nbsp;Xiaoxiao Gan,&nbsp;Ke Yang,&nbsp;Fan Zhang,&nbsp;Jie Zhu,&nbsp;Xia Yi","doi":"10.1007/s12010-026-05638-5","DOIUrl":"10.1007/s12010-026-05638-5","url":null,"abstract":"<div>\u0000 \u0000 <p>Zinc and magnesium were the important activators for enzyme catalysis and microorganism growth. However, their effect on the fermentability of <i>Monascus purpureus</i> remained unclear. This study assessed the effect of zinc and magnesium ion on ethanol and Monascus pigments synthesis in <i>M. purpureus</i> using pure glucose and xylose. With 1.0 g/L Zn (Ⅱ) amended, ethanol concentration was raised by 160.48% over the control at 1 d. Meanwhile, 0.0, 3.0 and 4.0 g/L Zn (Ⅱ) separately contributed to the increase of 71.00% for red pigments, 58.36% for yellow pigments and 86.67% for orange pigments. Affirmatively, 1.0, 2.5, 2.5 and 1.5 g/L Mg (Ⅱ) were separately benefit for the production of ethanol, red pigments, yellow pigments and orange pigments. Upon the co-supplementation of 1.0 g/L Zn (Ⅱ) and 1.0 g/L Mg (Ⅱ) initially intended to enhance ethanol concentration, the content of red pigments, yellow pigments and orange pigments were unexpectedly increased separately by 141.85%, 59.44% and 97.49% simultaneously with ethanol increased by 964.76%. Zn (Ⅱ) served as the primary stimulus for ethanol production. Additionally, the co-supplementation of the above metal ion contributed to forty-seven differentially expressed genes (DEGs), especially with alcohol dehydrogenase (ADH) for glycolysis pathway for ethanol production, serine hydrolase (MPsGeG), FAD dependent monooxygenase (MPsGeN) and FAD dependent oxidoreductase (MPsGeF) for polyketide pathway for Monascus pigments synthesis and specific transporters for the transport of sugars and other molecules intensified, and thus further indicated that the co-supplementation of zinc and magnesium ion improved ethanol and Monascus pigments synthesis in <i>M. purpureus</i> at transcriptional level. This work would offer a potential tactics through co-supplementing zinc and magnesium ion in fermentation system and candidate genes for genetic modification of <i>M. purpureus</i> to efficiently produce bioethanol and Monascus pigments.</p>\u0000 </div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 5","pages":"4013 - 4033"},"PeriodicalIF":3.3,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Significance and Immune Landscape of Migrasome-Related Genes in Pancreatic Cancer","authors":"Ben Liu,&nbsp;Shuang Li,&nbsp;Wenzhe Gao,&nbsp;Hongwei Zhu,&nbsp;Pin Lyu,&nbsp;Xiao Yu,&nbsp;Yuting Xiao","doi":"10.1007/s12010-026-05649-2","DOIUrl":"10.1007/s12010-026-05649-2","url":null,"abstract":"<div><p>Pancreatic cancer is characterized by early metastasis and high aggressiveness, highlighting the need for novel molecular biomarkers to improve prognostic assessment and therapeutic strategies. In this study, we constructed a migrasome-related prognostic model through multiple cohorts using 101 machine learning algorithms, and applied Shapley Additive Explanations (SHAP) to interpret the model’s feature contributions. Six key genes — tetraspanin 5 (TSPAN5), bone morphogenetic protein 1 (BMP1), integrin subunit alpha 3 (ITGA3), integrin subunit alpha 5 (ITGA5), Wnt family member 11 (WNT11), and tetraspanin 2 (TSPAN2) —were identified, mainly enriched in extracellular matrix (ECM)–receptor interaction and cell adhesion pathways. Further analyses showed that these genes were preferentially expressed in tumor cells and fibroblasts, and were associated with an altered immune microenvironment. Drug sensitivity prediction and molecular docking indicated that the monocarboxylate transporter 1 (MCT1) inhibitor AZD-3965 may have therapeutic potential in this context. In general, our findings suggest that migrasome-related genes may contribute to prognostic stratification of pancreatic cancer and point to mechanisms of stroma–immune crosstalk, thereby offering exploratory avenues for personalized treatment.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 5","pages":"3975 - 3990"},"PeriodicalIF":3.3,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Intracellular Trafficking of the Low Molecular Weight Protamine in Breast Cancer Cells","authors":"Ismail Sami Mahmoud,&nbsp;Majed Al Holi,&nbsp;Walhan Alshaer","doi":"10.1007/s12010-026-05622-z","DOIUrl":"10.1007/s12010-026-05622-z","url":null,"abstract":"<div>\u0000 \u0000 <p>Low molecular weight protamines (LMWPs) are polycationic peptide fragments that possess a high capability of penetrating cells with low toxicity. These peptides have been utilized to produce innovative pharmaceutical formulations for gene delivery systems and nanopharmaceutical particles. Despite the potential impact of LMWPs for therapeutic applications their subcellular trafficking pathways, particularly the translocation to the nuclear/nucleolar region, is not completely understood. Targeting delivery of drugs to these compartments could be utilized to treat diseases like breast cancer to increase drug efficiency, reduce side toxicity and overcome drug resistance mechanisms. In this study, the dynamic trafficking of the LMWP fragment (Pep2) to the nuclear/nucleolar compartment has been analyzed in breast cancer cell lines using laser scanning confocal microscopy and live-cell imaging techniques. Also, the present study determines a key part of the LMWP trafficking: a potential nuclear/nucleolar localization sequence (NLS/NoLS) that could be associated with the peptide. Indeed, we have identified the specific sequence (RRRRRGGRRRR) as a potential NLS/NoLS that could be required by the LMWP fragment (Pep2) for its trafficking and accumulation in the nuclear/nucleolar compartments. The functionality of the sequence has been validated using mutagenesis experiments combined with microscopic analysis in cells. The findings of this study could be utilized to improve the efficiency of various LMWP-containing biopharmaceutical formulations, probably through modulation of their subcellular localization.</p>\u0000 </div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 5","pages":"3991 - 4012"},"PeriodicalIF":3.3,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CSE Induces Lung Epithelial Cell-derived Exosomal LINC01133-mediated FOXA1 Activity Through SRSF6 to Affect MRC-5 Cellular Lipid Metabolism and Autophagy","authors":"Yong Zhou,&nbsp;Yuhan Zhu,&nbsp;Ying Zhou,&nbsp;Zhongkai Tong,&nbsp;Xiaoxiao Zhu,&nbsp;Xuekui Du,&nbsp;Zhaoxing Dong","doi":"10.1007/s12010-026-05644-7","DOIUrl":"10.1007/s12010-026-05644-7","url":null,"abstract":"<div>\u0000 \u0000 <p>Smoking is an important risk factor for the pathogenesis of idiopathic pulmonary fibrosis (IPF). Cigarette smoke (CS) damages alveolar epithelial cells and induces them to release exosomes (Exos). Although exosomal LINC01133 has been shown to regulate epithelial–mesenchymal transition (EMT), its effect and mechanism of action in IPF remain unclear. The present study aimed to investigate the effect and molecular mechanism of exosomal LINC01133 in IPF. Bleomycin (BLM) was injected into the trachea of mice to establish an IPF animal model, and the mice were treated with CS or Exos. In addition, 5% cigarette smoke extract (CSE) was added to the culture medium of type II alveolar epithelial cells (AT2) to induce the secretion of Exos. Western blot and RT–qPCR analyses were performed to detect the expression of related proteins or genes, and a CCK-8 assay, immunofluorescence staining, MDC staining, HE staining, and Masson’s staining were conducted to evaluate MRC-5 cell activation and mouse lung tissue fibrosis. The result shows that CSE-induced AT2 cells secreted exosomal LINC01133, which promoted the expression of α-SMA and lipid metabolism-related markers (DGAT2, MTP, FATP2, ACAT2, GPAT1, and APOB) in MRC-5 cells in vitro. Exosomal LINC01133 decreased the expression of autophagy-related proteins (ATG7, Beclin1, and LC3Ⅱ/I) and resulted in abnormal activation of MRC-5 cells. Exosomal LINC01133 also promoted lipid accumulation and inhibited autophagy in lung fibroblasts in vivo. Mechanistically, exosomal LINC01133 inhibited FOXA1/UCP1 signaling by upregulating SRSF6 expression, which promoted lipid accumulation and inhibited autophagy, ultimately activating lung fibroblasts and accelerating the progression of IPF in mice. Overall, CSE-induced AT2 cell-derived exosomal LINC01133 promotes the activation of lung fibroblasts and accelerates the progression of IPF.</p>\u0000 </div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 5","pages":"3954 - 3974"},"PeriodicalIF":3.3,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repurposing Lapatinib and Tucatinib as Dual Inhibitors of Bcl-2 and TYMS in Breast Cancer: Insights from Transcriptomic, Computational and Cellular Assays","authors":"Mehreen Aftab,&nbsp; Shagufta,&nbsp;Sandeep Sisodiya,&nbsp;Asiya Khan,&nbsp;Sandeep Kumar,&nbsp;Pranay Tanwar,&nbsp;Showket Hussain","doi":"10.1007/s12010-026-05618-9","DOIUrl":"10.1007/s12010-026-05618-9","url":null,"abstract":"<div><p>Breast cancer remains a leading cause of cancer related deaths worldwide. HER2-positive subtypes, marked by HER2 receptor over expression often exhibit aggressive behavior. Advances in high-throughput sequencing have revealed new molecular targets, paving the way for more precise therapies. Targeted therapies inhibit HER2 signaling, improving clinical outcomes. However, tumour resistance and survival pathways remain challenges. Identifying alternative targets of HER2 inhibitors may uncover new pathways to suppress tumour growth and improve treatment efficacy. Publicly available RNA-Seq datasets were processed in Galaxy to profile differential gene expression. Bcl-2, an anti-apoptotic regulator and TYMS, a key enzyme in DNA synthesis have emerged as lead candidates. Twenty literatures derived HER2 inhibitors were docked against both proteins, and 100 ns molecular dynamics simulations refined binding stability and validated by functional in-vitro cellular assays. Lapatinib showed the strongest binding to Bcl-2, while tucatinib had the lowest binding energy for TYMS. Both drugs displayed binding profiles resembling their respective controls. Lapatinib and tucatinib are both targeted therapies primarily used to treat HER2-positive cancers. Our study suggests that these agents may also exert effects beyond their primary targets, including potential roles in modulation of Bcl-2 for lapatinib and TYMS inhibition for tucatinib. Furthermore, we observed correlation between Bcl-2 and TYMS expression in breast cancer suggesting that their interplay may influence tumour progression, prognosis, and therapy responsiveness. Our study highlights that lapatinib and tucatinib, beyond HER2 inhibition, may also target Bcl-2 and TYMS respectively. This dual functionality could improve therapeutic strategies against HER2-positive breast cancer. Further biochemical and clinical validation will be necessary to confirm their mechanistic significance.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 5","pages":"3929 - 3953"},"PeriodicalIF":3.3,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Exosomal Hsa_circ_0005692 as A Novel Biomarker for Colorectal Cancer Metastasis: A Retrospective Observational Study","authors":"Jingjing Zhang,&nbsp;Zhengquan Han,&nbsp;Yajuan Han,&nbsp;Xianfei Xing,&nbsp;Meng Xu","doi":"10.1007/s12010-026-05641-w","DOIUrl":"10.1007/s12010-026-05641-w","url":null,"abstract":"<div>\u0000 \u0000 <p>Background. Increasing evidence suggests that exosomal circular RNAs (circRNAs) could serve as promising novel biomarkers for colorectal cancer (CRC) detection. However, diagnostic potential of exosomal circRNAs in CRC metastasis remain largely underexplored. Methods. The differentially expressed circRNAs (DEcircRNAs) were screened through GSE159669 and GSE205643 datasets. Seventy patients with CRC and seventy age- and sex-matched healthy controls were retrospectively enrolled in this study. The expression of DEcircRNAs was validated in paired cancer and paracancerous tissues, as well as in serum and serum-derived exosomes by RT-qPCR. The clinical significance, prognostic, and diagnostic efficacy of DEcircRNAs were evaluated through chi-square test, Kaplan-Meier survival curves, Cox regression model analysis, and receiver operating characteristic curves. The effect of DEcircRNAs on CRC cell migration and invasion was assessed through wound healing and transwell assays with gain- and loss-of-function methods. Results. A total of six DEcircRNAs were identified, among which only hsa_circ_0005692 exhibited consistent upregulation in cancer tissues, serum, and serum-derived exosomes from patients with CRC. Increased exosomal hsa_circ_0005692 was positively associated with lymph node metastasis and distant metastasis. Patients with elevated levels of exosomal hsa_circ_0005692 exhibited lower overall survival and progression-free survival rates, and exosomal hsa_circ_0005692 was identified as an independent risk factor for poor overall survival. Notably, exosomal hsa_circ_0005692 showed superior diagnostic accuracy than its expression in tissues and serum counterparts in differentiating not only CRC patients from healthy controls, but also metastatic patients from non-metastatic patients. Moreover, hsa_circ_0005692 was found to facilitate CRC metastasis by promoting cell migration and invasion as well as epithelial-mesenchymal transition. Conclusion. This study provides preliminary evidence that serum exosomal hsa_circ_0005692 may serve as a potential biomarker for predicting metastasis in CRC patients, and functionally facilitates CRC metastasis. However, larger-scale confirmatory studies and external validation are still required to substantiate this conclusion.</p>\u0000 </div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 5","pages":"3910 - 3928"},"PeriodicalIF":3.3,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}