Simple and Cost-effective Fluoride Riboswitch-Based Whole-Cell Biosensor for the Determination of Fluoride in Drinking Water.

IF 3.1 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Applied Biochemistry and Biotechnology Pub Date : 2025-05-24 DOI:10.1007/s12010-025-05269-2

Madushani R Ariyarathna, Jayani P Nissanka, Kasun Methlal, Kasun D Abeyrathne, Madhusha Satharasinghe, P Banushan, Dilhara Manawadu, Gayan Mirihana Arachchilage, Gayathri N Silva

引用次数: 0

Abstract

Fluoride in drinking water poses health risks when exceeding WHO-recommended limits, necessitating accurate detection methods. This study developed a highly selective fluoride riboswitch (FRS)-based whole-cell biosensor using an E. coli mutant with a recombinant plasmid carrying the FRS fused to the lacZ reporter gene. Fluoride binding to the FRS aptamer triggers β-galactosidase expression, enabling fluoride quantification by a colorimetric assay. Using a calibration curve (R² > 0.9), fluoride concentrations in Sri Lankan water samples were determined (0.15-0.90 ppm). Minimal interference from common ions (Cl⁻, OH⁻, Na⁺, K⁺, Ca²⁺, CO₃²⁻, HCO₃⁻, and NO₃⁻) confirmed the biosensor's narrow specificity and high selectivity towards fluoride.

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基于氟化物核糖开关的全细胞生物传感器用于饮用水中氟化物的测定。

饮用水中的氟化物如果超过世卫组织建议的限度，就会对健康构成威胁，因此需要采用准确的检测方法。本研究利用大肠杆菌突变体和携带FRS融合到lacZ报告基因的重组质粒，开发了一种基于高选择性氟核糖开关（FRS）的全细胞生物传感器。氟化物与FRS适体结合触发β-半乳糖苷酶表达，使氟化物能够通过比色法定量。使用校准曲线（R2 > 0.9），测定了斯里兰卡水样中的氟化物浓度（0.15-0.90 ppm）。常见离子（Cl⁻，OH⁻，Na⁺，K⁺，Ca2⁺，CO₃2⁻，HCO₃⁻和NO₃⁻）的干扰很小，证实了生物传感器对氟化物的窄特异性和高选择性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学

CiteScore

5.70

自引率

6.70%

发文量

460

审稿时长

5.3 months

期刊介绍： This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.