Applied Biochemistry and Biotechnology最新文献

{"title":"An Optimized Droplet Digital PCR Assay for HER2 Copy Number Variation in Breast Cancer Based on Multi-reference Genes.","authors":"Jinbing Xue, Houshi Ma, Xiaoliang Zhang, Shun Wang, Jinxian Wang, Zeqin Li, Xi Wu, Tianhang Yang, Changsong Zhang, Gangyin Luo","doi":"10.1007/s12010-025-05233-0","DOIUrl":"https://doi.org/10.1007/s12010-025-05233-0","url":null,"abstract":"<p><p>Targeted therapy is essential for the 15-30% invasive breast cancer patients with human epidermal growth factor receptor 2 (HER2) over-expression. However, current HER2 diagnosing methods rely on complex manual works and highly subjective interpretations. To more accurately and objectively assess the HER2 amplification status of formalin fixed paraffin embedded (FFPE) samples, a droplet digital PCR (ddPCR) assay based on multi-reference genes was developed. We established a four-fluorescence ddPCR assay using breast cancer cell lines (T-47D and SK-BR-3) and validated it on 101 clinical breast cancer FFPE samples. Compared to clinicopathological results, the ddPCR assay based on two out of three reference genes demonstrated superior sensitivity (82.6%), specificity (98.7%), and consistency (95.0%) in determining HER2 status over assays using single or three reference genes. Whole genome sequencing of the abnormal cases further confirmed that the ddPCR assay outperformed clinical immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and quantitative PCR (qPCR) in accuracy. Our findings demonstrate that the multi-reference gene ddPCR assay significantly improves the accuracy of HER2 status detection and reduces errors associated with chromosome 17 abnormalities. This method holds promise as a complementary or alternative approach to conventional IHC and FISH testing in tissue biopsies and is also feasible for liquid biopsies.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Polystyrene Microplastics on Mitochondrial Oxidative Damage in Renal and Muscular Tissues of the Freshwater Fish.","authors":"Moothedath Arya, Kuttichira Jithila, Reeha Mashirin, Kumari Chidambaran Chitra","doi":"10.1007/s12010-025-05236-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05236-x","url":null,"abstract":"<p><p>Polystyrene microplastics (PS-MPs), an emerging environmental contaminant, have attracted significant concern in recent years. This study aimed to evaluate mitochondrial oxidative damage in renal and muscular tissues of the freshwater fish Anabas testudineus following exposure to environmentally relevant concentrations (13.6 mg L⁻¹ and 23.6 mg L⁻¹) of PS-MPs for 1, 7, and 15 days. Exposure to PS-MPs disrupted the antioxidant defense system within the mitochondrial compartments of renal and muscle tissues, leading to increased levels of hydrogen peroxide generation and lipid peroxidation. PS-MPs exposure altered metabolic functions in the mitochondrial fractions of kidney and muscle tissues, as evidenced by elevated activities of alanine aminotransferase and aspartate aminotransferase. Besides, exposure to PS-MPs resulted in a decline of tissue-specific marker enzymes, such as acid and alkaline phosphatases, in renal tissue, indicating tissue damage. Histological examinations revealed significant tissue damage, including necrosis of renal tubules, vacuolization, glomerular degeneration, and melanomacrophage aggregation in kidney tissues. In muscle tissues, the observed damages included myolysis, vacuolar degeneration, necrosis, and atrophy of muscle fibers. These findings suggest that oxidative stress induced by PS-MPs exposure disrupts metabolic functions in mitochondrial fractions, thereby providing valuable insights into nephrotoxicity and muscular toxicity in fish.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics Analysis Reveals Hub Genes Linked to Programmed Cell Death in Intervertebral Disc Degeneration.","authors":"Mingyang Zou, Shaobo Wu, Jundan Wang, Wenya Xue, Xince Sun, Luyu Liu, Pan Yin, Dageng Huang","doi":"10.1007/s12010-025-05243-y","DOIUrl":"https://doi.org/10.1007/s12010-025-05243-y","url":null,"abstract":"<p><p>Intervertebral disc degeneration (IVDD) represents a severe chronic condition characterized by diverse programmed cell death (PCD) mechanisms serving as critical pathological features. The identification of key genes associated with cellular demise in IVDD is crucial for enhancing diagnostic and prognostic strategies. We extracted microarray-based transcriptomic multi-datasets from the GEO database, comprising 34 normal specimens (grade I/II) and 38 IVDD cases (grade III/IV). Nineteen PCD-associated genes encompassing multiple death modalities (including apoptosis, pyroptosis, ferroptosis, autophagy, necroptosis, cuproptosis, parthanatos, entotic cell death, netotic cell death, lysosome-dependent cell death, alkaliptosis, oxeiptosis, NETosis, immunogenic cell death, anoikis, paraptosis, methuosis, entosis, and disulfidptosis) were systematically curated from established studies. Pathway enrichment was evaluated through gene set variation analysis (GSVA), while weighted gene co-expression network analysis (WGCNA) facilitated the identification of core cell death-related genes, ultimately constructing a cell death signature (CDS) risk model via LASSO regression. Then, we found the significant upregulation of specific PCD pathways in IVDD specimens, particularly apoptosis, ferroptosis, autophagy, necroptosis, immunogenic cell death, anoikis, and disulfidptosis. Immune profiling revealed substantial infiltration of M0 macrophages in IVDD tissues, contrasting with predominant activated NK cells and M2 macrophages in control groups. Through integrative analysis by limma and WGCNA, we discerned 19 key PCD-related genes, subsequently identifying three gene targets (YWHAB, BID, and GSDME) for IVDD pathogenesis. This investigation culminated in developing a machine learning-driven prognostic model based on these biomarkers. Our study establishes a novel and comprehensive framework integrating IVDD with PCD mechanisms, proposing YWHAB, BID, and GSDME as promising diagnostic biomarkers and therapeutic targets for IVDD management.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of Ulva rigida for Fabrication of Iron Oxide Nanoparticles and Its Physicochemical Characterization.","authors":"Rajiv Periakaruppan, Kavya Govindharaj, Joaval Antony Martin, Karungan Selvaraj Vijai Selvaraj, Noura Al-Dayan","doi":"10.1007/s12010-025-05253-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05253-w","url":null,"abstract":"<p><p>Ulva species is consumed macroalgae, and it has rich polysaccharides. Nanoparticles play an important role in medicine, agriculture, and environment. Extracts from bacteria, algae, and plants can be used for synthesis of nanoparticles fields. The study determines the biogenic synthesis and characterization of iron oxide nanoparticles by U. rigida extract. The size, nature, stability, and composition of green synthesized iron oxide nanoparticles were characterized using UV-Vis, XRD, FTIR, FESEM, EDX, and TGA analysis. UV-Vis and FTIR analysis were performed to determine the surface plasmon resonance and occurrence of functional groups in the U. rigida-mediated iron oxide nanoparticles. FTIR spectra proved the formation of metal oxide functional groups. U. rigida-mediated iron oxide nanoparticles were spherical in shape with the average size of 50-60 nm and confirmed by FESEM analysis. Crystalline nature of U. rigida-mediated iron oxide nanoparticles was synthesized. A weight loss of 30.1% U. rigida-mediated iron oxide nanoparticles was observed in TGA analysis and confirms its high thermal stability. The study reveals that U. rigida extract can be used for stabilizing agents for fabrication of iron oxide nanoparticles. The synthesized iron oxide nanoparticles may be used in wastewater treatment and drug delivery system.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Chemotherapeutic Agents Used in Breast Cancer Treatment on the Hepatic Lipotoxicity.","authors":"Maghsoud Shaaker, Masoud Darabi, Gholamreza Dehghan, Mohammad Ali Hosseinpour Feizi, Amir Mehdizadeh","doi":"10.1007/s12010-025-05237-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05237-w","url":null,"abstract":"<p><p>Breast cancer (BC) is the most common type of cancer in women. Researchers have studied various types of inhibitors of the MAPK/ERK signaling pathway, which plays an important role in the growth of BC cells. PD98059 is a potent and selective inhibitor of MEK, which may have potential application in the combined treatment of BC along with doxorubicin and Taxotere. The aim of this study was to provide a new insight into hepatotoxicity caused by doxorubicin, Taxotere, and PD98059 mono/combination therapy in a hepatoma in vitro model. HepG2 cells were treated with appropriate doses of used drugs. The expression of FASN and SCD1 lipogenic genes was measured by real-time PCR. The fatty acid composition PL and triglyceride fractions were analyzed using gas-liquid chromatography. The indicators of oxidative stress and antioxidant defense systems were measured by the calorimetric method. Doxorubicin and Taxotere significantly decreased FASN expression and increased SCD1 expression. Additionally, the combination of PD98059 alone, in combination with doxorubicin, or doxorubicin and Taxotere significantly increased the expression of SCD1. However, the combination of PD98059 and Taxotere significantly decreased this expression. PD98059 alone also dramatically increased the expression of FASN. In the PL fraction, doxorubicin, Taxotere, or PD98059 increased the percentage of saturated fatty acids and decreased the relative amount of unsaturated fatty acids alone. The doxorubicin + PD98059 combination also enhanced the effect of doxorubicin on PL SFAs and UFAs. Taxotere also reduced the SFAs and increased UFAs in the triglyceride fraction, which was also neutralized by PD98059 addition. Based on these findings, increased expression of SCD1 and elevated levels of SFAs in lipid fractions may indicate the possibility of hepatic lipotoxicity in BC patients, which necessitates the narrow monitoring of steatohepatitis in these patients.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiRNA- 1293 Promotes Hepatocellular Carcinoma Cell Proliferation and Invasion by METTL3-Mediated m6 A Modification of Pri-miRNA- 1293.","authors":"Jingwen Liu, Beibe Zhu, Nenglie Jin, Jie Lv, Hongke Zhou","doi":"10.1007/s12010-025-05241-0","DOIUrl":"https://doi.org/10.1007/s12010-025-05241-0","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC) is a highly heterogeneous, proliferative, and aggressive malignancy of the digestive system. MicroRNAs (miRNAs) are expected to be a new target for the treatment of HCC. Crosstalk between N6-methyladenine (m6A) modification and miRNAs are involved in HCC progression. This study aimed to explore the role of miR-1293 and its underlying mechanism in HCC progression. The biological behaviors of HCC cells were analyzed by cell counting kit-8 and transwell assay. The underlying mechanism was determined by quantitative real-time PCR, methylated RNA immunoprecipitation (MeRIP), RIP, and xenograft tumor experiment. The results indicated that miR-1293 was highly expressed in HCC. Upregulated miR-1293 promoted the viability, invasion, and migration of HCC cells. Mechanically, the level of METTL3 and m6A modification was increased in HCC cells. METTL3 accelerated the processing and maturation of pri-miR-1293 in an m6A-dependent manner. Moreover, miR-1293 mimic reversed the inhibitory effect of METTL3 knockdown on HCC cellular biological behaviors. In addition, miR-1293 promoted tumor growth in vivo. This study revealed the regulatory role of miR-1293 in HCC is related to the participation of METTL3-mediated m6A methylation, which could provide new therapeutic strategies for HCC.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Goat Skin (Capra aegagruss Erxleben, 1777): a Promising and Sustainable Source of Collagen.","authors":"Jamile Maria Pereira Bastos Lira de Vasconcelos, Robson Coelho de Araújo Neri, Amanda Vieira de Barros, Carlos Eduardo Sales da Silva, Maria Cecília Ferreira Galindo, Bruno Oliveira de Veras, Ranilson Souza Bezerra, Maria Betânia Melo de Oliveira","doi":"10.1007/s12010-025-05242-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05242-z","url":null,"abstract":"<p><p>The aim of the study was to extract and characterize collagen from solid untanned skin waste from the processing of leather in a Capra aegagrus tannery. Using pepsin (SPC), 37 g of collagen were obtained from 100 g of dry weight skin. Characterization took place using SDS-PAGE, FTIR and UV absorption techniques, identifying it as type I collagen. The ultraviolet (UV) absorption spectrum showed a peak at 238 nm. In the thermogram, the maximum transition temperature was 56º C. Using the electrophoresis technique, it was observed that SPC consists of band patterns formed by a γ chain, a β chain and two distinct α chains (α1 and α2). In the FTIR analysis, the collagen showed the absorption peaks for the amides, showing that the SPC extraction process maintained the integrity of the molecule. To observe the effect of NaCl concentration on the solubility of SPC, the collagen showed high solubility, up to a concentration of 2% NaCl. The solubility peak was observed at pH 4.0, with a sharp drop until pH 7.0, reaching its minimum point at pH 10. Scanning microscopy showed some irregular surfaces, cavities and fibrous structures, which may favor the application of collagen as a biomaterial. The zeta potential found the isoelectric point of collagen at pH = 6.61. These results indicate that the collagen obtained has a high level of structural integrity and can be applied as an alternative source, as well as adding value to a waste product that is often discarded in the environment.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation Between CAV-1 and PTEN-Mediated Apoptosis in Hyperoxia-Induced Acute Lung Injury.","authors":"Xin Yi, Bing Li, Xiao Yu, Dawei Cao, Ting Xue, Yujing Zhao, Xinri Zhang","doi":"10.1007/s12010-025-05208-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05208-1","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Purpose: &lt;/strong&gt;Respiratory support is essential in the clinical management of critically ill patients; however, prolonged exposure to high concentrations of oxygen can result in hyperoxia-induced acute lung injury (HALI). In this study, we developed a model of hyperoxia exposure utilizing C57BL/6 mice and human bronchial epithelial (BEAS-2B) cells. We employed CAV-1 siRNA transfection and CAV-1 expression plasmid techniques to analyze the effects of hyperoxia on the expression of caveolin-1 (CAV-1), the deletion of the phosphatase and tensin homolog (PTEN) gene on chromosome 10, and the apoptotic markers Bax and Bcl-2. Additionally, we explored the mechanisms by which CAV-1 regulates PTEN-mediated apoptosis in the context of HALI. Our findings aim to provide valuable insights for developing effective preventive and therapeutic strategies to combat this condition.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;First, we established a hyperoxia-induced acute lung injury (HALI) model in male C57BL/6 mice. Histopathological examination was conducted using hematoxylin-eosin staining to evaluate the pathological changes and the severity of lung tissue damage. Next, we developed an in vitro HALI model utilizing the BEAS-2B cell line. Subsequently, CAV-1 siRNA and CAV-1 expression plasmids were transfected into BEAS-2B cells. We quantified the expression levels of CAV-1, PTEN, Bax, and Bcl-2 using reverse transcription polymerase chain reaction (RT-PCR) and immunoblotting techniques. Additionally, the impact of altered CAV-1 expression on apoptosis in BEAS-2B cells was assessed through flow cytometry.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Exposure to hyperoxia led to pathological alterations in mice's lung tissue, increased the CAV-1, PTEN, and Bax expression levels, and decreased Bcl-2 expression. Initially, there were no notable variances in the expression levels of CAV-1, PTEN, and Bax in the cells. However, as the exposure time to hyperoxia prolonged, there was a significant increase in both mRNA and protein expression levels of CAV-1 and PTEN, while Bcl-2 exhibited a significant decrease. Moreover, CAV-1 knockdown attenuated the expression of PTEN and Bax, and elevated the expression of Bcl-2. However, CAV-1 overexpression showed an opposite result. The expression levels of CAV-1, PTEN, and Bax were positively correlated in mice and cell models, and negatively correlated with those of Bcl-2. Additionally, downregulation of CAV-1 suppressed apoptosis in BEAS-2B cells.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Our results indicate that CAV-1 plays a pivotal role in regulating the expression of PTEN and the apoptosis-related factors Bax and Bcl-2 in a hyperoxic environment. This regulatory function of CAV-1 on PTEN and its downstream apoptotic pathways suggests a significant correlation between CAV-1 and PTEN-mediated apoptosis. Consequently, CAV-1 is involved in the development of hyperoxic lung injury (HALI) through the PTEN-mediated apoptotic pathway. These findi","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Nutritional Efficiency in Poultry Feed: Optimized Production and Immobilization of Thermostable Phytase from Mucor indicus Using Agricultural By-Products.","authors":"Swethaa Venkataraman, Kanishka Mohan Raj, Shreya Vivek, Ben Johnson, Vinoth Kumar Vaidyanathan","doi":"10.1007/s12010-025-05218-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05218-z","url":null,"abstract":"<p><p>In this study, a thermostable phytase enzyme was produced from Mucor indicus through the optimization of media components, followed by immobilization, with the aim of enhancing the nutritional value of broiler and layer feed via dephytinization process. Various agricultural by-products, including wheat bran, rice bran, chickpea husk, and black gram husk were evaluated for their efficacy in phytase production. Among these, black gram husk demonstrated the highest enzyme activity, achieving 92.10 U/ml. Optimization of media components and physical parameters, including a temperature of 50 °C, a pH of 5.5, an inoculum age of 72 h, an inoculum size of 1.5%, glucose as the carbon source, and peptone as the nitrogen source, resulted in a significant enhancement in enzyme activity, reaching 184.03 U/ml. The catalytic efficiency of the free enzyme was determined to be 5.68 ± 0.28 mM/s, whereas the immobilized enzyme exhibited a substantially higher catalytic efficiency of 17.26 ± 0.24 mM/s. Application of the immobilized enzyme for the dephytinization of broiler and layer feed led to phosphorus liberation of 35.45 mg/g and 58.46 mg/g, respectively, after 24 h of incubation. These results demonstrate the potential of immobilized thermostable phytase for improving the nutritional quality of animal feed by utilizing sustainable agricultural by-products.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerating the Understanding of Biosensors Through the Lens of Cells: State of the Field, Emerging Directions, Advances, and Challenges.","authors":"Jasjeet Narang, Niket Rana, Arjun Chauhan, Anushka Kumari, Vanshika Minhas","doi":"10.1007/s12010-025-05209-0","DOIUrl":"https://doi.org/10.1007/s12010-025-05209-0","url":null,"abstract":"<p><p>Cell-based biosensors are evolving as versatile tools for biological research, drug development, and environmental monitoring. Living cells are used to detect elements in these biosensors, which offer significant advantages over standard transducers. The purpose of this review article is to provide an in-depth overview of cell-based biosensors, emphasizing their working principles, fabrication processes, and applications. The potential of living cells to respond to particular analytes or stimuli supports the design and operation of cell-based biosensors. Real-time and label-free identification can be accomplished by combining these cells with transducers like microelectrodes or optical sensors. Genetically engineered cells or changed microenvironments can be used in cell-based biosensors to improve performance by optimizing cell types for increased dynamic range, sensitivity, and selectivity. Cell-based biosensors are developed by meticulously cultivating and immobilizing cells on transducer surfaces while retaining their vitality and performance. Cell-based biosensors have a wide range of applications, including monitoring the environment, healthcare, and pharmaceutical research. These biosensors have been used to detect diseases, toxic substances, pollutants, and therapeutic drug screening. Cell-based biosensors are cutting-edge technology that brings together the capabilities of live cells and transducers to detect analytes in a sensitive and specific manner. These biosensors illustrate the tremendous potential for upcoming uses in healthcare and monitoring environmental conditions with further developments in fabrication methods and the inclusion of artificial intelligence.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}