Applied Biochemistry and Biotechnology最新文献_第8页

Repercussions of Prolonged Pesticide Use on Natural Soil Microbiome Dynamics Using Metagenomics Approach 利用元基因组学方法研究长期使用杀虫剂对天然土壤微生物组动态的影响

IF 3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-03 DOI: 10.1007/s12010-024-05033-y

Bhumika Maharana, Sejal Mahalle, Rahul Bhende, Nishant A. Dafale

{"title":"Repercussions of Prolonged Pesticide Use on Natural Soil Microbiome Dynamics Using Metagenomics Approach","authors":"Bhumika Maharana, Sejal Mahalle, Rahul Bhende, Nishant A. Dafale","doi":"10.1007/s12010-024-05033-y","DOIUrl":"https://doi.org/10.1007/s12010-024-05033-y","url":null,"abstract":"The residual pesticides in soil can affect the natural microbiome composition and genetic profile that drive nutrient cycling and soil fertility. In the present study, metagenomic approach was leveraged to determine modulations in nutrient cycling and microbial composition along with connected nexus of pesticide, antibiotic, and heavy metal resistance in selected crop and fallow soils having history of consistent pesticide applications. GC–MS analysis estimated residuals of chlorpyrifos, hexachlorbenzene, and dieldrin showing persistent nature of pesticides that pose selective pressure for microbial adaptation. Taxonomic profiling showed increased abundance of pesticide degrading Streptomyces, Xanthomonas, Cupriavidus, and Pseudomonas across the selected soils. Genes encoding for pesticide degrading cytochrome p450, organophosphorus hydrolase, aldehyde dehydrogenase, and oxidase were predominant and positively correlated with Bacillus, Sphingobium, and Burkholderia. Nitrogen-fixing genes (nifH, narB, and nir) were relatively less abundant in crop soils, correlating to the decrease in nitrogen-fixing bacteria (Anabaena, Pantoea, and Azotobacter). Microbial enzymes involved in carbon (pfkA, gap, pgi, and tpiA) and phosphorus cycle (gmbh and phnJ) were significantly higher in crop soils indicating extensive utilization of pesticide residuals as a nutrient source by the indigenous soil microbiota. Additionally, presence of antibiotic and heavy metal resistance genes suggested potential cross-resistance under pressure from pesticide residues. The results implied selective increase in pesticide degrading microbes with decrease in beneficial bacteria that resulted in reduced soil health and fertility. The assessment of agricultural soil microbial profile will provide a framework to develop sustainable agriculture practices to conserve soil health and fertility.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"184 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Inflammatory Effects of Quercetin, Rutin, and Troxerutin Result From the Inhibition of NO Production and the Reduction of COX-2 Levels in RAW 264.7 Cells Treated with LPS 槲皮素、芦丁和曲克芦丁的抗炎作用源于抑制 LPS 处理的 RAW 264.7 细胞中 NO 的生成和 COX-2 水平的降低

IF 3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-03 DOI: 10.1007/s12010-024-05003-4

Gi Baek Lee, Yohan Kim, Kyung Eun Lee, Ramachandran Vinayagam, Mahendra Singh, Sang Gu Kang

{"title":"Anti-Inflammatory Effects of Quercetin, Rutin, and Troxerutin Result From the Inhibition of NO Production and the Reduction of COX-2 Levels in RAW 264.7 Cells Treated with LPS","authors":"Gi Baek Lee, Yohan Kim, Kyung Eun Lee, Ramachandran Vinayagam, Mahendra Singh, Sang Gu Kang","doi":"10.1007/s12010-024-05003-4","DOIUrl":"https://doi.org/10.1007/s12010-024-05003-4","url":null,"abstract":"Flavonols effectively scavenge the reactive nitrogen species (RNS) and reactive oxygen species (ROS) and act as immune-enhancing, anti-inflammatory, anti-diabetic, and anti-carcinogenic agents. Here, we explored the comparative antioxidant and anti-inflammatory properties of plant-originating flavonols, like quercetin, rutin, and troxerutin against acetylsalicylic acid. Quercetin and rutin showed a high ability to remove active ROS, but troxerutin and acetylsalicylic acid exhibited little such function. In RAW 264.7 cells, quercetin, rutin, and troxerutin did not exhibit cellular toxicity at low concentrations. In addition, quercetin, rutin, and troxerutin considerably (p < 0.05) lowered the protein expression of cyclooxygenase 2 (COX-2) as compared to acetylsalicylic acid in cells inflamed with lipopolysaccharides (LPS). Additionally, in inflamed cells, quercetin and rutin significantly down-regulated the nitrogen oxide (NO) level (p < 0.05) at higher concentrations, whereas Troxerutin did not reduce the NO level. In addition, Troxerutin down-regulated the pro-inflammatory protein markers, such as TNF-α, COX-2, NF-κB, and IL-1β better than quercetin, rutin, and acetylsalicylic acid. We observed that troxerutin exhibited a significantly greater anti-inflammatory effect than acetylsalicylic acid did. Acetylsalicylic acid did not significantly down-regulated the expression of COX-2 and TNF-α (p < 0.05) compared to troxerutin. Hence, it can be concluded that the down-regulation of NO levels and the expression of COX-2 and TNF-α proteins could be mechanisms of action for the natural compounds quercetin, rutin, and troxerutin in preventing inflammation.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"97 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct Gene Expression and Immune Features Between Different Neutrophil Extracellular Trap-Related Osteosarcoma Subtypes 不同中性粒细胞胞外捕获器相关骨肉瘤亚型的基因表达和免疫特征各不相同

IF 3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-03 DOI: 10.1007/s12010-024-05021-2

Delei Song, Xuqing Yin, Chunqing Che

{"title":"Distinct Gene Expression and Immune Features Between Different Neutrophil Extracellular Trap-Related Osteosarcoma Subtypes","authors":"Delei Song, Xuqing Yin, Chunqing Che","doi":"10.1007/s12010-024-05021-2","DOIUrl":"https://doi.org/10.1007/s12010-024-05021-2","url":null,"abstract":"We sought to determine neutrophil extracellular trap (NET)–related genes’ potential value in improving the efficacy of diagnosis and identifying novel therapeutic targets for osteosarcoma. Data were obtained from TARGET, GEO, and CCLE database. Differentially expressed genes were identified between the subtypes based on NET-related genes. PPI network was constructed using STRING, following by ClueGO enrichment analysis. Infiltration of immune cells was calculated by ssGSEA. Risk Score model was built by LASSO Cox regression analysis. Western blot and qRT-PCR were applied to validate the expression of genes used in the model. We identified 19 NET-related genes with prognostic potential in osteosarcoma using univariate Cox regression analysis. Patients from TARGET were clustered into two subtypes with distinct prognosis and immune features. 381 DEGs were identified between the two NET subtypes. Risk Score based on BST1, SELPLG, FPR1 and TNFRSF10C was reliable to predict the prognosis of osteosarcoma patients. The four genes expressed significantly lower in osteosarcoma than normal cells. Low Risk Score individuals only existed in C1 subtype with better prognosis. Osteosarcoma were clustered into two subtypes based on NET-related genes. Risk Score model constructed by four NET-related gene was able to independently predict the prognosis of osteosarcoma.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141886810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of SF1 and SF2 Helicases in Biotechnological Applications. SF1 和 SF2 螺旋酶在生物技术应用中的作用。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-02 DOI: 10.1007/s12010-024-05027-w

Jing Dai, Ronghui Liu, Shujun He, Tie Li, Yuhang Hu, Huiqun Huang, Yi Li, Xinrong Guo

{"title":"The Role of SF1 and SF2 Helicases in Biotechnological Applications.","authors":"Jing Dai, Ronghui Liu, Shujun He, Tie Li, Yuhang Hu, Huiqun Huang, Yi Li, Xinrong Guo","doi":"10.1007/s12010-024-05027-w","DOIUrl":"https://doi.org/10.1007/s12010-024-05027-w","url":null,"abstract":"Helicases, which utilize ATP hydrolysis to separate nucleic acid duplexes, play crucial roles in DNA and RNA replication, repair, recombination, and transcription. Categorized into the major groups superfamily 1 (SF1) and superfamily 2 (SF2), alongside four minor groups, these proteins exhibit a conserved catalytic core indicative of a shared evolutionary origin while displaying functional diversity through interactions with various substrates. This review summarizes the structures, functions and mechanisms of SF1 and SF2 helicases, with an emphasis on conserved ATPase sites and RecA-like domains essential for their enzymatic and nucleic acid binding capabilities. It highlights the unique 1B and 2B domains in SF1 helicases and their impact on enzymatic activity. The DNA unwinding process is detailed, covering substrate recognition, ATP hydrolysis, and conformational changes, while addressing debates over the active form of UvrD helicase and post-unwinding dissociation. More importantly, this review discusses the biotechnological potential of helicases in emerging technologies such as nanopore sequencing, protein sequencing, and isothermal amplification, focusing on their use in pathogen detection, biosensor enhancement, and cancer treatment. As understanding deepens, innovative applications in genome editing, DNA sequencing, and synthetic biology are anticipated.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141873805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lnc NBAT1 Inhibits the Proliferation and Migration of Liver Cancer Cells Through the miR-21/PDCD4/AP-1 Signaling Axis. Lnc NBAT1 通过 miR-21/PDCD4/AP-1 信号轴抑制肝癌细胞的增殖和迁移

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-02 DOI: 10.1007/s12010-024-05008-z

Hongyan Zai, Xin Wu, Yifan Zhou, Yu Hu, Qin Zhu

{"title":"Lnc NBAT1 Inhibits the Proliferation and Migration of Liver Cancer Cells Through the miR-21/PDCD4/AP-1 Signaling Axis.","authors":"Hongyan Zai, Xin Wu, Yifan Zhou, Yu Hu, Qin Zhu","doi":"10.1007/s12010-024-05008-z","DOIUrl":"https://doi.org/10.1007/s12010-024-05008-z","url":null,"abstract":"Long non-coding RNAs (Lnc RNAs) are proven to participate in liver cancer (LC) regulation. The regulation of miR-21 by lnc NBAT1 has been studied in other cancers. However, the effect of this regulation on LC and its specific mechanism remains unclear. Lnc NBAT1 and miR-21 expressions in clinical tissues were measured by RT-qPCR. PDCD4, AP-1, p-c-Fos, p-c-Jun, and cyclin D1 expressions were analyzed by Western blot. Overexpression of lnc NBAT1 was studied to explore its influence on malignant behaviors of Bel7402 cells and the development of LC in the xenograft mouse model (XMM). The regulation mechanism of lnc NBAT1 in LC was explored by lnc NBAT1 overexpression, miR-21 mimic treatment, or PDCD4 silencing in Bel7402 cells. Lnc NBAT1 expression was downregulated while miR-21 expression was upregulated in LC tissues and cell lines. In comparison with LX-2 cells, the expressions of PDCD4 and AP-1 were downregulated in Bel7402 cells, while those of p-c-Fos, p-c-Jun, and cyclin D1 were upregulated. Further, lnc NBAT1 was found to localize primarily in the cytoplasm of Bel7402 cells. Overexpression of lnc NBAT1 enhanced cell apoptosis, blocked the cell cycle, suppressed malignant behaviors of Bel7402 cells, and inhibited tumor progression in the XMM. Mechanistically, lnc NBAT1 functioned as a competing endogenous RNA (ceRNA) by binding to the downstream target miR-21 to stabilize the expressions of PDCD4 and AP-1, thereby inhibiting malignant behaviors of Bel7402 cells. Lnc NBAT1 suppressed malignant behaviors of LC cells through the miR-21/PDCD4/AP-1 axis. Lnc NBAT1 might be a promising biomarker for LC treatment.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141873802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of the Lipid-Lowering Probiotic Lactiplantibacillus Plantarum SDJ09 and its Anti-Obesity Mechanism. 植物乳杆菌 SDJ09 的降脂益生菌筛选及其抗肥胖机制

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-02 DOI: 10.1007/s12010-024-05034-x

Baoxin Yang, Wenxuan Wang, Cuiwen Jian, Beibei Lv, Hailin He, Miao Wang, Shubo Li, Yuan Guo

{"title":"Screening of the Lipid-Lowering Probiotic Lactiplantibacillus Plantarum SDJ09 and its Anti-Obesity Mechanism.","authors":"Baoxin Yang, Wenxuan Wang, Cuiwen Jian, Beibei Lv, Hailin He, Miao Wang, Shubo Li, Yuan Guo","doi":"10.1007/s12010-024-05034-x","DOIUrl":"https://doi.org/10.1007/s12010-024-05034-x","url":null,"abstract":"In this study, 39 strains of lactic acid bacteria were screened from several fermented foods. Based on the evaluation of functional and prebiotic properties, Lactiplantibacillus plantarum SDJ09 was selected as a promising candidate. It gave a 48.16% cholesterol reduction and 33.73% pancreatic lipase inhibition in cells; exhibited high resistance to acid, bile salts, and gastrointestinal fluid; and had strong antibacterial activity and high adhesion capabilities. More importantly, the lipid-lowering effect of L. plantarum SDJ09 was also investigated using 3T3-L1 mature adipocytes and HepG2 nonalcoholic fatty liver disease models. L. plantarum SDJ09 effectively decreased triglyceride accumulation by more than 50% in both cell models, in which the expression of PPARγ, C/EBPα, aP2, and LPL in 3T3-L1 cells was significantly downregulated by L. plantarum SDJ09. L. plantarum SDJ09 also improved lipid metabolism by downregulating the expression of HMGCR, SREBP-1c, ACC, and FAS and upregulating the expression of CYP7A1 in HepG2 nonalcoholic steatohepatitis cells. Therefore, L. plantarum SDJ09 has the potential to effectively decrease obesity and non-alcoholic fatty liver disease (NAFLD) by inhibiting lipid accumulation, providing a prospective probiotic agent for anti-obesity.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141873804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process Optimization for Biosurfactant Production by Bacillus aryabhattai SPS1001 using Taguchi Method. 使用田口方法优化ryabhattai SPS1001 杆菌生产生物表面活性剂的工艺。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-02 DOI: 10.1007/s12010-024-05019-w

Farkhunda Fatima, Nishi Prakash Tiwari, Varsha Singh

{"title":"Process Optimization for Biosurfactant Production by Bacillus aryabhattai SPS1001 using Taguchi Method.","authors":"Farkhunda Fatima, Nishi Prakash Tiwari, Varsha Singh","doi":"10.1007/s12010-024-05019-w","DOIUrl":"https://doi.org/10.1007/s12010-024-05019-w","url":null,"abstract":"This study employs Taguchi design of experiments (DOE) to optimize biosurfactant yield by analyzing the impact of various input parameters. Signal-to-noise ratio analysis was utilized for optimization, corroborated by ANOVA findings. Regression equations depicted response behaviour and are validated through a confirmation test. Taguchi methodology identified optimal conditions for maximum biosurfactant yield: agitation (180 rpm), inoculum size (2%), beef extract (5 g/L), diesel (20 ml/L), peptone (5 g/L), NaCl (7 g/L), incubation time (4 days), pH (7.9), and yeast extract (6 g/L). This yielded an 8.33% increase to 1.53 g/L, with initial optimum parameters projecting 1.41 g/L. ANOVA ranked and quantified control factor contributions, revealing agitation's significant (31.41%) impact on yield. The study underscores the viability of Taguchi's optimal conditions for substantial yield improvement within specific ranges. The strong alignment between expected and experimental yields affirmed the reliability of developed models for optimal yield selection. This study underscores the power of statistical techniques like Taguchi DOE and ANOVA in systematically enhancing biosurfactant production by Bacillus aryabhattai SPS1001 and paves the way for future advancements in bioprocess optimization.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141873803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Integrated Metabolomics Study on Antidiabetic Activity of Christia vespertilionis Leaves Extract Using Chemometric and Molecular Docking Analysis. 利用化学计量学和分子对接分析对鸢尾叶提取物抗糖尿病活性的综合代谢组学研究

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-01 DOI: 10.1007/s12010-024-04998-0

Puvana Devi Selvarajoo, Alfi Khatib, Mohd R Razali, Ahmed Mediani, Suganya Murugesu, Vikneswari Perumal

{"title":"An Integrated Metabolomics Study on Antidiabetic Activity of Christia vespertilionis Leaves Extract Using Chemometric and Molecular Docking Analysis.","authors":"Puvana Devi Selvarajoo, Alfi Khatib, Mohd R Razali, Ahmed Mediani, Suganya Murugesu, Vikneswari Perumal","doi":"10.1007/s12010-024-04998-0","DOIUrl":"https://doi.org/10.1007/s12010-024-04998-0","url":null,"abstract":"In disease treatment, the utilisation of medicinal plants has witnessed a discernible rise, driven by concerns over the adverse effects associated with synthetic drugs available in the market. Analyses of the plant Christia vespertilionis (L.f.) Bakh. F., indigenous to Malaysia, has suggested its antidiabetic property linked to α-glucosidase inhibition, but metabolites responsible for antidiabetic are unexplored. The metabolomics approaches and molecular docking simulations were integrated to identify the putative α-glucosidase inhibitors and their enzyme interaction. In this study, the crude leaves extracted from this plant were extracted using solvents of varying polarity, followed by gas and liquid chromatography coupled with mass spectrometry metabolomics. The highest inhibition activity in a mixture of n-hexane and ethyl acetate (1:1, v/v)) was observed. Six putative metabolites corresponding to antidiabetic activity were identified: palmitic acid (2), linolenic acid (4), 7-tetradecenal (5), aloeemodin-8-monoglucoside (14), bruceine I (15), and sanjidin B (16). The mechanism of action of all the identified compounds is competitive, mainly involving hydrophobic and hydrogen bonding interactions with the protein residues. Compounds 14, 15, and 16 exhibited strong binding capabilities with both enzyme crystal structures compared to the positive control, quercetin. The metabolites extracted from C. vespertilionis leaves have demonstrated promising antidiabetic effects. These antidiabetic compounds can potentially commercialise new drug candidates in managing diabetes conditions.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosurfactant Production by Pseudomonas: a Systematic Review. 假单胞菌的生物表面活性剂生产：系统综述。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-01 DOI: 10.1007/s12010-024-05036-9

Larissa de Souza Araújo, Larice Aparecida Rezende Santana, Marcelo Henrique Otenio, Clerison Wagner Nascimento, Ana Flávia Lawall Werneck Cerqueira, Mirian Pereira Rodarte

{"title":"Biosurfactant Production by Pseudomonas: a Systematic Review.","authors":"Larissa de Souza Araújo, Larice Aparecida Rezende Santana, Marcelo Henrique Otenio, Clerison Wagner Nascimento, Ana Flávia Lawall Werneck Cerqueira, Mirian Pereira Rodarte","doi":"10.1007/s12010-024-05036-9","DOIUrl":"https://doi.org/10.1007/s12010-024-05036-9","url":null,"abstract":"It is of fundamental interest to research and develop innovative biotechnologies, as well as bioproducts that replace or are alternatives to those of non-renewable origin, such as biosurfactants in relation to traditional surfactants used in various sectors. Consequently, there are a large number of experimental studies addressing different subjects, especially with the use of bacteria of the genus Pseudomonas; however, there is a lack of work that demonstrates the evaluation of this science produced to date. Therefore, this article discusses the production of biosurfactants by Pseudomonas with the aim of surveying and analyzing experimental articles on this topic. To realize this, a systematic search was carried out with well-defined temporal space, databases, and inclusion and exclusion criteria, based on metric studies that guided what information would be collected and the method of evaluation. Therefore, a large number of articles were selected, which demonstrated Pseudomonas aeruginosa as the bioagent mostly used in the tests, which aimed to improve the process in the area. Furthermore, interest in this field has increased over the years, predominantly in emerging market countries, where the most prominent authors on the topic are found. Therefore, it is necessary that there is an expansion of interest in the area to make the production of biosurfactants cheaper in areas that currently have greater development deficiencies, such as means of purifying the bioprocess and reducing foam formation in the bioprocess.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and Application of a Multienzyme System for Synthesis of L-malate. 合成 L-苹果酸的多酶系统的构建与应用。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-01 DOI: 10.1007/s12010-024-05026-x

Jielin Zhao, Xiaolian Li, Ranfeng He, Yunshan Wang, Ziqiang Wang

{"title":"Construction and Application of a Multienzyme System for Synthesis of L-malate.","authors":"Jielin Zhao, Xiaolian Li, Ranfeng He, Yunshan Wang, Ziqiang Wang","doi":"10.1007/s12010-024-05026-x","DOIUrl":"https://doi.org/10.1007/s12010-024-05026-x","url":null,"abstract":"This study aimed to develop a multienzymatic system for synthesis of L-malate. First, recombinant Escherichia coli strains were constructed expressing maleic acid cis-trans isomerase (MaiA) or fumarase C (FumC) from different sources. Serratia marcescens MaiA (SMaiA) and E. coli FumC (ECFumC) showed good catalytic performance. Next, six co-expression systems for SMaiA and ECFumC were constructed. E. coli BL21 (DE3)-pRSFDuet-1-ecfumC-smaiA (named strain pFM2) had the highest L-malate catalytic activity. In 7-L fed-batch fermentation, the SMaiA and ECFumC activities of strain pFM2 wet cells were 43.4 and 154.5 U/g, respectively, 2.4- and 10.7-fold the values that were obtained in shaken flasks. Finally, a whole-cell catalytic process was established for the production of L-malate by strain pFM2 with maleate as the substrate. When the dose of pFM2 wet cells was 0.5 g/100 mL and 1 mol/L maleate was the substrate, the catalytic process was completed within 4 h. Notably, the intermediate fumarate was almost absent during the conversion process. The concentration of L-malate reached 143.8 g/L with a yield of 0.60 g/(L·min). The molar conversion rate of the substrate was 98.4%. These findings lay a foundation for the industrial application of multienzymatic synthesis of L-malate.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0