Applied Biochemistry and Biotechnology最新文献_第8页

Renoprotective Effects of Phloretin and TUDCA via Simultaneous Inhibition of TLR4/MyD88/NF-κB and BiP/PERK/CHOP Pathways in AKI Under Diabetic Condition. 根皮素和TUDCA通过同时抑制TLR4/MyD88/NF-κB和BiP/PERK/CHOP通路在糖尿病AKI中的肾保护作用

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-03 DOI: 10.1007/s12010-025-05315-z

Vishwadeep Shelke, Neha Dagar, Anil Bhanudas Gaikwad

{"title":"Renoprotective Effects of Phloretin and TUDCA via Simultaneous Inhibition of TLR4/MyD88/NF-κB and BiP/PERK/CHOP Pathways in AKI Under Diabetic Condition.","authors":"Vishwadeep Shelke, Neha Dagar, Anil Bhanudas Gaikwad","doi":"10.1007/s12010-025-05315-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05315-z","url":null,"abstract":"The diabetic milieu increases the chances of acute kidney injury (AKI) progression. During AKI, activation of the inflammatory response via Toll-like receptor 4 (TLR4) and endoplasmic reticulum (ER) stress progress in kidney dysfunction. Moreover, emerging evidence suggests a functional interplay between TLR4 signaling and ER stress in kidney disease. However, the effect of simultaneous inhibition of these mechanisms has not yet been studied in AKI under diabetic settings. In this study, we investigated the renoprotective effect of Phloretin-TLR4 inhibitor and Tauro ursodeoxycholic acid (TUDCA)-ERS inhibitor in AKI under diabetic condition. Using a bilateral ischemia-reperfusion injury (BIRI) model in streptozotocin-induced diabetic rats and a high glucose cultured sodium azide-induced injury model in NRK52E cells, we evaluated the effects of both agents administered alone and in combination. In rats, phloretin at 50 mg/kg/p.o. and TUDCA at 400 mg/kg/p.o. alone and in combination were administered for 5 days before surgery, while in NRK52E cells, both drugs were given 24 h before hypoxia. Pretreatment with phloretin and TUDCA significantly attenuated renal dysfunction, preserved tissue architecture, and reduced markers of inflammation and apoptosis (p < 0.05). Mechanistic analysis revealed that phloretin suppressed the TLR4/MyD88/NF-κB signaling pathway, while TUDCA inhibited ER stress via the BiP/PERK/CHOP axis. Notably, combination therapy exhibited a synergistic effect (p < 0.05), offering superior renoprotection compared to monotherapy. Our findings suggest that targeting both TLR4-induced inflammation and ER stress simultaneously offers a promising therapeutic strategy for mitigating AKI in diabetic settings and may pave the way for novel combination treatments in diabetic kidney disease.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering exosomes for liver disease: a new insight in regenerative medicine and drug delivery. 肝脏疾病的工程外泌体：再生医学和药物输送的新见解。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-03 DOI: 10.1007/s12010-025-05309-x

Dakshina M Nair, Leela Kakithakara Vajravelu, Jayaprakash Thulukanam, Rahul Harikumar Lathakumari, Poornima Baskar Vimala, Vishnupriya Paneerselvam

{"title":"Engineering exosomes for liver disease: a new insight in regenerative medicine and drug delivery.","authors":"Dakshina M Nair, Leela Kakithakara Vajravelu, Jayaprakash Thulukanam, Rahul Harikumar Lathakumari, Poornima Baskar Vimala, Vishnupriya Paneerselvam","doi":"10.1007/s12010-025-05309-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05309-x","url":null,"abstract":"Exosomes, nanoscale extracellular vesicles derived from endosomes, have emerged as crucial mediators of intercellular communication, significantly influencing physiological balance and disease development. Their biogenesis occurs via two different pathways-ESCRT-dependent and ESCRT-independent mechanisms-that regulate the selective packing of lipids, proteins, and nucleic acids. This review offers a mechanistic understanding of exosome production, cargo sorting, and secretion, highlighting their dynamic regulation in response to cellular stress conditions. Exosomes in the liver promote metabolic control, immunological modulation, fibrosis development, and the pathogenesis of hepatocellular carcinoma via modulating interactions among hepatocytes, Kupffer cells, and hepatic stellate cells. Furthermore, exosomes function as potential diagnostic biomarkers, with their molecular content indicative of disease conditions such as viral hepatitis, non-alcoholic fatty liver disease (NAFLD), and hepatocellular cancer. Recent advancements in exosome engineering, including targeted surface alterations, hybrid vesicle technologies, and hydrogel-based delivery methods, have shown their therapeutic promise for precision medicine. However, challenges such as heterogeneity in exosome isolation, cargo variability, off-target effects, and immunogenicity pose translational barriers. The standardization of isolation procedures, enhancement of cargo-loading tactics, and establishment of regulatory frameworks are essential for their clinical use. Overcoming these restrictions will enable exosome-based precision diagnostics and therapies, establishing them as leaders in next-generation regenerative medicine and tailored drug delivery. This study distinctly highlights the use of modified exosomes in liver disorders, integrating biogenesis mechanisms with novel treatment approaches and delivery systems.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PFKM Promotes the Progression of Gastric Cancer by Up-regulating CNTN1 Expression Through H3K18la Modification. PFKM通过H3K18la修饰上调CNTN1表达，促进胃癌进展。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-03 DOI: 10.1007/s12010-025-05319-9

Xin Shen, Hai Shi, Lei Liu, Shujing Chong, Junwei Han, Yuping Gu

{"title":"PFKM Promotes the Progression of Gastric Cancer by Up-regulating CNTN1 Expression Through H3K18la Modification.","authors":"Xin Shen, Hai Shi, Lei Liu, Shujing Chong, Junwei Han, Yuping Gu","doi":"10.1007/s12010-025-05319-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05319-9","url":null,"abstract":"Gastric cancer (GC) stands as one of the most common malignancies globally, characterized by significant incidence rates. Phosphofructokinase muscle isoform (PFKM), a critical rate-limiting enzyme in glycolysis, has its expression modulated by lactate production in tumor cells. The objective of this study is to elucidate the underlying molecular mechanisms by which PFKM contributes to the pathogenesis of GC. The viability, migration, and invasion of GC cells were analyzed by CCK-8 and transwell assays. Each condition was repeated three times. The regulation of H3K18la on transcription activity of CNTNl was evaluated by·dua-luciferase reporter assay. Animal experiment was performed using nude mice with six mice in each group, and tumor growth was evaluated. Statistical analysis was performed using GraphPad Prism software with t-test, one-way or two-way ANOVA. We found that PFKM was over-expressed in GC. Downregulated PFKM restrained the viability, migration, invasion, glucose uptake, and lactate production of GC cells. Mechanically, PFKM interacted with CNTN1 and facilitated the enrichment of H3K18la at the CNTN1 promoter region. Overexpression of CNTN1 reversed the inhibitory effects of PFKM knockdown on GC progression. Our research showed that increasing PFKM levels accelerated GC development by regulating CNTN1 expression through mechanisms involving histone lactylation, which could potentially contribute to novel approaches in diagnosing and treating GC.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Cloning, Optimization of Expression and Functional Characterization of the Global Transcriptional Regulator MosR (Rv0348) of Mycobacterium tuberculosis. 结核分枝杆菌全局转录调控因子MosR （Rv0348）的克隆、表达优化及功能表征

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-03 DOI: 10.1007/s12010-025-05299-w

Dheeraj Singh, Rohitth Raj Eb, Amitava Bandhu

{"title":"Molecular Cloning, Optimization of Expression and Functional Characterization of the Global Transcriptional Regulator MosR (Rv0348) of Mycobacterium tuberculosis.","authors":"Dheeraj Singh, Rohitth Raj Eb, Amitava Bandhu","doi":"10.1007/s12010-025-05299-w","DOIUrl":"10.1007/s12010-025-05299-w","url":null,"abstract":"In-vivo microarray analysis on murine model of Tuberculosis had identified a novel transcriptional regulator MosR, which regulates the expression of ~ 163 genes, many of which are important for intracellular persistence of dormant Mycobacterium tuberculosis. MosR therefore, may be considered as potential target for anti-tuberculosis drug discovery. In this work, this important regulator has been cloned, expressed, purified to homogeneity, and characterized to some extent. MosR is found to cooperatively bind within the ORF of the previous gene rv0347. The environmental conditions for most favorable interaction between MosR and its cognate DNA are determined to be 8.0 pH, 25 mM NaCl, and 25 ℃ temperature. The equilibrium dissociation constant (Kd) for MosR-DNA interaction is determined to be 0.23 ± 0.02 µM under the optimized conditions. MosR is composed of mostly α-helices with minor β-sheets and β-turns as major secondary structural elements. Interestingly, MosR is found to harbor a highly conserved homeodomain and a long N-terminal arm which might be involved in binding to specific DNA, supported by the predicted three-dimensional structure. The tertiary structure of MosR is found to be stable under different pH and salt concentrations while secondary structure undergoes distinct conformational changes. MosR is unable to maintain its structure and DNA-binding activity at a temperature more than 35 ℃ and forms microaggregate in solution which suggests that it is a moderately thermosensitive protein. These information would be useful for in-vitro screening and validations of inhibitors against MosR in a high throughput manner.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KAT3B Protects Against Septic Lung Injury by Regulating TSLP Succinylation and Macrophage Polarization. KAT3B通过调节TSLP琥珀酰化和巨噬细胞极化保护脓毒性肺损伤。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-02 DOI: 10.1007/s12010-025-05314-0

Chao Li, Jialiang Zhang, Fuhong Zheng, Haibo Liu

{"title":"KAT3B Protects Against Septic Lung Injury by Regulating TSLP Succinylation and Macrophage Polarization.","authors":"Chao Li, Jialiang Zhang, Fuhong Zheng, Haibo Liu","doi":"10.1007/s12010-025-05314-0","DOIUrl":"https://doi.org/10.1007/s12010-025-05314-0","url":null,"abstract":"As a serious complication of sepsis, septic lung injury has a high rate of morbidity and mortality. However, the mechanism of septic lung injury remain unclear. The present study aims to explore the role of succinylation and KAT3B in the polarization of macrophages and the progression of septic lung injury. The cecal ligation and puncture (CLP) was used to establish the septic lung injury model. HE staining was performed to evaluate the lung injury. Raw264.7 cells were treated with lipopolysaccharide (LPS) to establish the cell model of septic lung injury. Macrophage polarization was evaluated by detecting M1 and M2 markers using quantitative real-time polymerase chain reaction and immunofluorescence. CO-IP was used to investigate the interaction between proteins. The experimental results indicated that KAT3B and the total succinylation was down-regulated in septic lung injury. LPS promoted M1 polarization and inhibited M2 polarization of Raw264.7 cells. KAT3B suppressed M1 polarization and promoted M2 polarization. Mechanistically, KAT3B desuccinylates TSLP at K239 and K292 site and promotes its stability. TSLP silencing reversed the effects of KAT3B. In vivo, KAT3B significantly reduced the lung injury and inflammation response of mice after CLP treatment. The present research unveiled the KAT3B acts as a protector against sepsis induced injury by mediating the succinylation of TLSP.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Alkaline Pretreatment and Structural Insights for Converting Eucalyptus Sawdust and Sugarcane Bagasse into Cellulosic Sugars. 桉木屑和甘蔗渣制纤维素糖的碱预处理优化及结构研究。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-02 DOI: 10.1007/s12010-025-05316-y

Mario Alberto Yaverino-Gutierrez, Jesús Jiménez Ascencio, Anuj Kumar Chandel

{"title":"Optimization of Alkaline Pretreatment and Structural Insights for Converting Eucalyptus Sawdust and Sugarcane Bagasse into Cellulosic Sugars.","authors":"Mario Alberto Yaverino-Gutierrez, Jesús Jiménez Ascencio, Anuj Kumar Chandel","doi":"10.1007/s12010-025-05316-y","DOIUrl":"https://doi.org/10.1007/s12010-025-05316-y","url":null,"abstract":"The efficient conversion of lignocellulosic biomass into fermentable sugars is critical for the sustainable production of biofuels and bioproducts. This study optimized mild alkaline pretreatment conditions for eucalyptus sawdust (ES) and sugarcane bagasse (SCB) using a Response Surface Methodology-Central Composite Rotational Design (RSM-CCRD). The effects of NaOH concentration, solid loading, temperature, and retention time on enzymatic hydrolysis efficiency were evaluated. Optimal pretreatment conditions (7.5% NaOH, 5% solid loading, 90 °C, 8 h) led to a tenfold increase in total reducing sugars (TRS) from ES (40.4 g/L) after 72 h, while SCB exhibited a higher hydrolysis efficiency (89.2%) and TRS (60.8 g/L) after enzymatic hydrolysis. Structural analyses using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) revealed enhanced cellulose accessibility and lignin modification in pretreated SCB, whereas ES remained more recalcitrant due to its higher lignin content. These findings demonstrate the effectiveness of mild alkaline pretreatment for SCB and highlight the need for more aggressive conditions to improve the digestibility of hardwood biomass. This study contributes to optimizing pretreatment strategies to enhance sugar release from agro-industrial residues, supporting lignocellulosic biorefinery development.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Potential of Co-supplementation of Zinc and Ferrous Iron Ion for Itaconic Acid Fermentation of Aspergillus terreus. 土曲霉衣康酸发酵中共添加锌和亚铁离子的潜力评价。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-01 DOI: 10.1007/s12010-025-05317-x

Xinji Li, Jianqi Han, Zhidan Liu, Xiaohui Shi, Caiwen Zhang, Xiuyun Zhao, Jie Zhu, Xia Yi

{"title":"Evaluating the Potential of Co-supplementation of Zinc and Ferrous Iron Ion for Itaconic Acid Fermentation of Aspergillus terreus.","authors":"Xinji Li, Jianqi Han, Zhidan Liu, Xiaohui Shi, Caiwen Zhang, Xiuyun Zhao, Jie Zhu, Xia Yi","doi":"10.1007/s12010-025-05317-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05317-x","url":null,"abstract":"Zinc (Zn) and ferrous iron (Fe) are the essential micronutrients for the growth of microorganisms. Nonetheless, whether they affect the fermentation of Aspergillus terreus or not remains poorly understood. This study assessed the effect of co-supplementation of zinc and ferrous iron ion on itaconic acid production for A. terreus fermenting pure glucose and xylose. In single factor assays, with mycelium growth and sugar consumption improved, itaconic acid concentration was increased by 63.87% at 2 days and 29.07% at 4 days for 0.25 g/L Zn (II) and 60.90% and 50.69% for 0.40 g/L Fe (II). The highest itaconic acid productivity and yield were separately 0.17 ± 0.01 g/L·h and 0.54 ± 0.01 g/g at 8 h for 0.25 g/L Zn (II) and 0.17 ± 0.00 g/L·h and 0.55 ± 0.01 g/g for 0.40 g/L (II). Co-supplementation of the optimized concentration of Zn (II) and Fe (II) brought about the increase of 60.56% and 71.37% for itaconic acid accumulation, and thus indicated that itaconic acid fermentation required the appropriate concentration of zinc and ferrous iron ion. Furthermore, through transcriptomic sequencing, the co-supplementation of zinc and ferrous iron ion contributed to 249 differentially expressed genes (DEGs) especially with the improved gene transcriptional for sugar transporter (Glut), transketolase (Tkt), mitochondrial tricarboxylate transporter (MttA), and major facilitator superfamily protein (MfsA) in biosynthetic pathway of itaconic acid, and thus would be responsible for the improved itaconic acid fermentability for A. terreus. This work would establish the improvement tactics for itaconic acid biosynthesis through co-supplementing zinc and ferrous iron ion in fermentation system and also provide synthetic biology tools for genetic modification of A. terreus to efficiently produce itaconic acid.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Submerged Fermentation Conditions of Aspergillus niger for Chitin Production. 黑曲霉深层发酵产几丁质条件的优化。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-01 DOI: 10.1007/s12010-025-05323-z

Giovana Marchezi, Gustavo Concolato, Luciane Maria Colla, Jeferson Steffanello Piccin

{"title":"Optimizing Submerged Fermentation Conditions of Aspergillus niger for Chitin Production.","authors":"Giovana Marchezi, Gustavo Concolato, Luciane Maria Colla, Jeferson Steffanello Piccin","doi":"10.1007/s12010-025-05323-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05323-z","url":null,"abstract":"Chitin, a biopolymer precursor to chitosan, can be obtained from fungal sources. To ensure viable and scalable production, the use of alternative culture media and the optimization of cultivation parameters are essential. This study aimed to optimize the biomass production of Aspergillus niger via submerged fermentation to obtain chitin. The composition of the culture media and the bioprocess time were evaluated using a Factorial Design 24. A subsequent Mixture Design was used to optimize carbon and nitrogen source concentrations, with fungal biomass and chitin yield as response variables. Scale-up was performed in a 5-L bioreactor under optimized conditions. As a result, the culture medium containing 7.5% wheat bran and 2.5% yeast extract was selected for cultivating A. niger, the predicted values for this condition were 4.40 g L-1 of fungal biomass and 25.24% of chitin yield. After scaling up to a bioreactor, it was possible to obtain 10.20 g L-1 and 4.95 g L-1 ± 0.06 of biomass and chitin, after 84 h of bioprocess. The study demonstrates the potential of using alternative culture media for the submerged fermentation of A. niger and chitin production, with the possibility of increasing yields in the scale-up of this bioprocess.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of Dilute Acid, Alkaline, and Deep Eutectic Solvent Pretreatments on Enzymatic Hydrolysis of Sunflower Stalk Bark. 稀酸、碱性和深共晶溶剂预处理对向日葵茎皮酶解的比较评价。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-07-01 DOI: 10.1007/s12010-025-05311-3

Chaoyue Zhang, Lu Cheng, Xin Zhou, Shuiping Ouyang

{"title":"Comparative Evaluation of Dilute Acid, Alkaline, and Deep Eutectic Solvent Pretreatments on Enzymatic Hydrolysis of Sunflower Stalk Bark.","authors":"Chaoyue Zhang, Lu Cheng, Xin Zhou, Shuiping Ouyang","doi":"10.1007/s12010-025-05311-3","DOIUrl":"https://doi.org/10.1007/s12010-025-05311-3","url":null,"abstract":"In this study, in order to overcome the recalcitrance of sunflower stalk bark (SSB) residues, three kinds of pretreatment methods, i.e., dilute acid pretreatment, alkaline pretreatment, and deep eutectic solvent (DES) pretreatment, were employed to pretreat the SSB material. The effects of the different pretreatment methods for enzymatic hydrolysis efficiency and sugar recovery were evaluated. The enzymatic hydrolysis yield (EHY) of SSB after dilute sulfuric acid, NaOH, and DES pretreatment could be improved from 15.2% to 26.8%, 96.7%, and 93.3%, respectively. Among the three pretreatment methods, dilute acid could effectively decompose hemicellulose while preserving more lignin in the pretreated solids, and it could only enhance EHY within a limited range (< 30%). Compared with dilute sulfuric acid pretreatment, NaOH and choline chloride/lactic acid pretreatments could effectively remove not only hemicellulose but also lignin. Results showed that satisfactory EHY (> 90%) was observed in both alkaline and DES pretreated SSB solids. Besides, DES method was much more efficient in the extraction of lignin (95%) compared with NaOH pretreatment (80.2%). Lignin could be recovery by DES pretreatment as value-added by-product from SSB and was more environmentally friendly and sustainable during production. Finally, under the condition of 1:10 (w/w) solid to DES solution and 150 °C pretreatment temperature, the mass balance of DES-150 °C showed that 28.1 g glucose was obtained from 100 g SSB, and the glucose recovery yield was 77.6% of theoretical, demonstrating the potential of environmentally friendly DESs in biorefinery.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photo-Induced Synthesis of Silver Nanoparticles Using Bacillus safensis as Elicitors for Enhanced Growth of Fenugreek in Hydroponic. 以沙芽孢杆菌为激发子光诱导合成纳米银促进胡芦巴水培生长。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-06-30 DOI: 10.1007/s12010-025-05313-1

Linta Jadoon, Alvina Gul, Hunaiza Fatima, Rabia Amir, Faiza Munir, Mustafeez Mujtaba Babar

{"title":"Photo-Induced Synthesis of Silver Nanoparticles Using Bacillus safensis as Elicitors for Enhanced Growth of Fenugreek in Hydroponic.","authors":"Linta Jadoon, Alvina Gul, Hunaiza Fatima, Rabia Amir, Faiza Munir, Mustafeez Mujtaba Babar","doi":"10.1007/s12010-025-05313-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05313-1","url":null,"abstract":"Utilizing renewable biological resources is an ecofriendly and sustainable approach as it minimizes the use of toxic chemicals and their environmental impact. In this study, stable silver nanoparticles (AgNPs) were biosynthesized using a low-cost, eco-friendly, photo-induced method. The cellular extract of Bacillus safensis strain U17-1 bacteria served as a reducing agent. Characterization of the biosynthesized AgNPs was conducted via scanning electron microscopy, confirming their spherical morphology, and EDS analysis conducted at 3 keV revealed the presence of silver. FTIR analysis identified various functional groups present on the surface of nanoparticles. The biosynthesized AgNPs had an average diameter of 133 nm, with a zeta potential of - 22.3 mV, establishing their stable, nanoparticulate nature. These biosynthesized AgNPs were utilized as nano-elicitors, and their elicitation effect was observed on the growth and phytochemical content of hydroponically grown Fenugreek plants. Our results demonstrated a significant increase in the following parameters: shoot height, fresh weight, dry weight, proteins, carbohydrates, phenolics, flavonoids, and tannins across various concentrations. These findings suggest the potential utility of biosynthesized AgNPs as elicitors for enhancing plant growth and phytochemical content, emphasizing their cost-effectiveness, and promising applications for sustainable agricultural practices.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0