Applied Biochemistry and Biotechnology最新文献_第8页

Engineering Bacterial Laccase with Improved Catalytic Activity and Thermostability by Rational Design. 通过合理设计提高催化活性和热稳定性的工程细菌漆酶。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-09 DOI: 10.1007/s12010-025-05240-1

Xuting Sun, Xiaofan Lin, Yufan Xian, Faying Zhang, Lingxuan Zhu, Haitao Geng, Wenya Wang, Guimin Zhang

{"title":"Engineering Bacterial Laccase with Improved Catalytic Activity and Thermostability by Rational Design.","authors":"Xuting Sun, Xiaofan Lin, Yufan Xian, Faying Zhang, Lingxuan Zhu, Haitao Geng, Wenya Wang, Guimin Zhang","doi":"10.1007/s12010-025-05240-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05240-1","url":null,"abstract":"Laccases (benzenediol:oxygen oxidoreductases) are important multi-copper oxidases with widespread applications in industry. Here, Bacillus subtilis laccase CotA that has been widely studied was engineered to improve catalytic activity and thermostability via rational design. After iterative mutation of beneficial mutation sites, a triple mutant of CotA laccase (DTA) was obtained, whose catalytic activity and thermostability were improved by 2.7-fold and 1.4-fold compared with the wild-type (WT) CotA, respectively. The enhanced activity of DTA is primarily due to strengthened intermolecular forces in the active site, while its improved thermostability is attributed to increased hydrophobic residues, augmented protein surface flexibility, collectively rendering DTA a more active and stable enzyme with potential industrial applications. Compared to WT, DTA can degrade mycotoxins aflatoxin B1 (55.09% vs 44.51%) and ZEN (zearalenone) (71.59% vs 41.09%) more efficiently, and DTA can also better pretreat lignocellulose, promoting the hydrolysis of cellulose by cellulase. All these indicate that DTA has the potential to be used in industry.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and Risk Assessment of Polycyclic Aromatic Hydrocarbons in the Surface Water of Budhabalanga River Estuary, Chandipur, East Coast of India. 印度东海岸昌迪普尔布哈巴兰加河河口地表水中多环芳烃的赋存及风险评价

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-08 DOI: 10.1007/s12010-025-05248-7

Subhashree Nayak, Smruti Prajna Pradhan, Pratyusha Nayak, Sthitaprajna Nath Sharma, Nishigandha Muduli, Susri Nayak, Lipika Patnaik

引用次数: 0

Effect of Sarpogrelate on Cerebral Venous Sinus Thrombosis Model. 沙棘酸酯对脑静脉窦血栓形成模型的影响。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-07 DOI: 10.1007/s12010-025-05234-z

Xichen Wan, Ye Xiong, Shi Zhou Xing, Xiao Wu, Song Xiao, Peng Liu

{"title":"Effect of Sarpogrelate on Cerebral Venous Sinus Thrombosis Model.","authors":"Xichen Wan, Ye Xiong, Shi Zhou Xing, Xiao Wu, Song Xiao, Peng Liu","doi":"10.1007/s12010-025-05234-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05234-z","url":null,"abstract":"To elucidate the effects of cerebral venous sinus thrombosis (CVST) on mice and the effects of sarpogrelate (S) on CVST, mice were randomly divided into the sham-operated group, CVST group, and CVST + S group (sarpogrelate). Neurological function was evaluated using the rotarod test, balance beam test, and open-field test. Moreover, laser speckle contrast imaging was employed to observe the blood flow in the cerebral cortex of mice, and immunofluorescence was used to quantify the neurons. In the neurofunctional assessment tests (i.e., rotarod test, balance beam test, and open-field test), the CVST group exhibited poorer performance compared to the sham-operated group. However, mice treated with sarpogrelate showed significantly better performance than the CVST group (P < 0.05). Moreover, a significant decrease in cerebral blood flow was observed in the CVST group compared to the sham-operated group (P < 0.01). In contrast, a significant increase in blood flow was observed in the CVST + S group compared to the CVST group (P < 0.05). Furthermore, the number of neurons in the hippocampus and prefrontal cortex of mice in the CVST group was lower than that in the sham group (P < 0.05), and the number of neurons in the hippocampus and prefrontal cortex of the CVST + S group was higher than that of the CVST group (P < 0.05). Sarpogrelate can increase blood flow in the cerebral cortex of mice with CVST, leading to decreased neuronal cell damage and improved neurological function.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Antibacterial, Antioxidant, Cytotoxic Potential of Oscillatoria sp.-Derived Metabolites and Their Molecular Docking with Estrogen Receptor-α. 探讨振荡藻衍生代谢物的抗菌、抗氧化、细胞毒性及其与雌激素受体-α的分子对接。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-06 DOI: 10.1007/s12010-025-05246-9

Santhosh Sigamani, Balasubramani Govindasamy, Balashanmugam Panneerselvam, Jothimani Kannupaiyan, Sivanandam Magudeeswaran, Dhandapani Ramamurthy, Hemalatha Natarajan

{"title":"Exploring Antibacterial, Antioxidant, Cytotoxic Potential of Oscillatoria sp.-Derived Metabolites and Their Molecular Docking with Estrogen Receptor-α.","authors":"Santhosh Sigamani, Balasubramani Govindasamy, Balashanmugam Panneerselvam, Jothimani Kannupaiyan, Sivanandam Magudeeswaran, Dhandapani Ramamurthy, Hemalatha Natarajan","doi":"10.1007/s12010-025-05246-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05246-9","url":null,"abstract":"Anthropogenic activities have led to the accumulation of carcinogenic toxicants in the food chain, posing severe risks to both human and animal health. Bioactive molecules derived from terrestrial and aquatic systems offer promising solutions to various health challenges. Presently, the cyanobacterial metabolites from ethyl acetate extract of Oscillatoria sp. (EAEOs) were evaluated for its antiproliferative, antioxidant, and antimicrobial effects. The EAEOs resulted significant antibacterial zones (12 ± 2.5, 17 ± 1.7, 14 ± 0.3, and 14 ± 2.6 mm) on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Bacillus cereus, respectively. EAEOs also exhibited free radical scavenging activity with IC50 values of 69.68 µg/mL (DPPH) and 144.4 µg/mL (hydroxyl radicals). Followed by, the EAEOs also exhibited significant inhibition of MCF-7 breast cancer cells (IC50: 54.25 µg/mL) compared to HepG2 and A549 cells (72.6 and 85.6 µg/mL), respectively The EAEOs induced apoptosis and chromosomal damage on targeted cancer cells, and their DNA fragmentation were evidenced. Totally, 17 compounds from EAEOs were identified from GC-MS, in which 2-cyclopenten-1-one, 2-hydroxy-3-methyl (23); piperidine, 1, 4-dimethyl (13); 17-pentatriacontene (7.88); and 3-octadecene (7.09%) are major ones. Molecular docking confirmed that acetamide, n-(2-benzoyl-4chlorophenyl)-2-(2-methylpiperidin-1-yl)- (compound-3) showed strong binding affinity (10.13 kcal/mol) with ERα. Overall, the biological potential of Oscillatoria metabolites were found to be significant. Hence, further screening of preponderant compounds and studying the mechanism are warranted to fulfill their applications in food, pharmaceuticals, and other industries.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trigonelline Shields Chondrocytes from Oxidative Damage in Osteoarthritis through Activation of the Keap1/Nrf2/ARE Signaling Pathway. 葫芦巴碱通过激活Keap1/Nrf2/ARE信号通路保护骨关节炎软骨细胞免受氧化损伤。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-05 DOI: 10.1007/s12010-025-05232-1

Chunmei Jiang, Xiaohong He, Aiju Lou, Shiwen He, Qixin Xie, Yuechun Wang, Shan Zhong, Weirong Wu, Qingchun Huang

{"title":"Trigonelline Shields Chondrocytes from Oxidative Damage in Osteoarthritis through Activation of the Keap1/Nrf2/ARE Signaling Pathway.","authors":"Chunmei Jiang, Xiaohong He, Aiju Lou, Shiwen He, Qixin Xie, Yuechun Wang, Shan Zhong, Weirong Wu, Qingchun Huang","doi":"10.1007/s12010-025-05232-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05232-1","url":null,"abstract":"Oxidative stress-induced chondrocyte damage is a key contributor to the progression of osteoarthritis (OA). While trigonelline (TG) possesses anti-inflammatory and antioxidant activities, its functional role and underlying mechanisms in OA remain unclear. In this study, the human chondrocyte cell line CHON-001 was treated with TG alone or in combination with IL-1β or ML385 for 24 h. Chondrocyte injury-related events were assessed using Cell Counting Kit-8 (CCK-8), flow cytometry with Annexin V-FITC/PI kit, Hoechst staining, the probe 2,7-Dichlorofluorescin diacetate (DCFH-DA), SA-β-gal staining, and SOD and MDA assay kits. Our data revealed that TG alleviated IL-1β-induced inflammation, apoptosis, extracellular matrix (ECM) degradation, senescence, and oxidative stress in chondrocytes, accompanied by the downregulation of Keap1 and upregulation of Nrf2, HO-1 and NQO1. ML385 treatment reversed the protective effects of TG against IL-1β-induced injury in chondrocytes. In vivo, the anterior cruciate ligament transection (ACLT) was used to induce a rat OA model, and TG was administered by gavage. OA severity and articular cartilage degradation were evaluated by hematoxylin and eosin (H&E), toluidine blue, Safranin-O staining, and Osteoarthritis Research Society International (OARSI) scoring system. The in vivo data showed that TG attenuated the degeneration and erosion of articular cartilage, suppressed inflammation, and downregulated the levels of Keap1 and iNOS, while upregulating the levels of Nrf2 and Col2a1. In conclusion, our study demonstrated that TG inhibits oxidative stress-induced chondrocyte dysfunction and cartilage degradation by activating the Keap1/Nrf2/ARE signaling pathway.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Role of Microbes in Remediation of High Molecular Weight Polycyclic Aromatic Hydrocarbon Persistence in the Environment. 揭示微生物在环境中高分子量多环芳烃持久性修复中的作用。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-03 DOI: 10.1007/s12010-025-05245-w

Tony Hadibarata, Kitara Pranareswari Hadibarata

{"title":"Unraveling the Role of Microbes in Remediation of High Molecular Weight Polycyclic Aromatic Hydrocarbon Persistence in the Environment.","authors":"Tony Hadibarata, Kitara Pranareswari Hadibarata","doi":"10.1007/s12010-025-05245-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05245-w","url":null,"abstract":"The persistence and recalcitrance of high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) are potential threats to health and the environment. They result mainly from incomplete combustion of fossil fuels and organic materials, and they tend to accumulate in terrestrial and aquatic ecosystems (particularly in soils, sediments, and water sources). Chronic exposure to HMW PAHs is associated with some of the most dreadful health outcomes, including lung and skin cancers and disorders of the respiratory and immune systems. The study therefore proposes microbial degradation as a promising bioremediation technique for HMW PAHs: pyrene, benzo[a]pyrene, chrysene, and fluoranthene. Aerobic degradations mediated by dioxygenase and dehydrogenase enzymes, as well as anaerobic pathways involving sulfate- and nitrate-reducing bacteria, are discussed. Factors that promote microbial degradation include pH, temperature, nutrient availability, and salinity. While all factors can be biostimulation and bioaugmentation, the study emphasizes these two as effective methods to enhance bioavailability and degradation efficiency. The results provide insightful information for further development of microbial techniques in remediation of HMW PAH-contaminated sites.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaerobic Volatile Fatty Acid Production Performance and Microbial Community Characteristics from Solid Fraction of Alkali-Thermal Treated Waste-Activated Sludge: Focusing on the Effects of Different pH Conditions. 碱热处理废活性污泥固体馏分厌氧挥发性脂肪酸生产性能及微生物群落特征：不同pH条件的影响

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-03 DOI: 10.1007/s12010-025-05244-x

Xiu-Fang Li, Wen-Shuai Zhang, Sheng Qi, Jun-Feng Zhao, Zhao-Yong Sun, Yue-Qin Tang

{"title":"Anaerobic Volatile Fatty Acid Production Performance and Microbial Community Characteristics from Solid Fraction of Alkali-Thermal Treated Waste-Activated Sludge: Focusing on the Effects of Different pH Conditions.","authors":"Xiu-Fang Li, Wen-Shuai Zhang, Sheng Qi, Jun-Feng Zhao, Zhao-Yong Sun, Yue-Qin Tang","doi":"10.1007/s12010-025-05244-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05244-x","url":null,"abstract":"The waste-activated sludge (WAS) is rich in organic matter and various nutrients. Alkali-thermal hydrolysis of WAS can be employed to produce a liquid fertilizer with high plant-promoting nutrient content. However, the solid fraction (abbreviated as SF) generated from this process requires further treatment. Although there have been studies on the recovery of plant nutrients from WAS via alkali-thermal hydrolysis, researches on the safe treatment of the SF are limited. This study aims to explore the potential and the microbiological mechanisms on anaerobic volatile fatty acid (VFA) production from the SF under different pH conditions (i.e., 6, 7, 8, 9, and 10). The results showed that the VFA yield was highest at pH 6, reaching 4095.84 mg COD/L (i.e., 0.16 g-COD/g-volatile solids), followed by pH 10, 8, 7, and 9, with acetate being the main component (> 56%). Microbial community analysis revealed that members in phyla Firmicutes and Bacteroidota constituted the main acid-producing microbial community during the anaerobic fermentation of SF. Furthermore, different pH conditions influenced the yield and composition of VFAs by altering the structure and functions of microbial community. This research provides a new direction for the fully resourceful utilization of sludge by producing both liquid fertilizer and VFAs from WAS.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decolorization of Azo and Anthraquinone Dyes Using Recombinant Horseradish Peroxidase A2A Isoenzyme Produced by Komagataella phaffii. 重组辣根过氧化物酶A2A同工酶对偶氮和蒽醌染料的脱色研究。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-03 DOI: 10.1007/s12010-025-05239-8

Nurgul Abul, Seyda Yildiz Arslan, Yagmur Unver, Hasan Ozdemir

{"title":"Decolorization of Azo and Anthraquinone Dyes Using Recombinant Horseradish Peroxidase A2A Isoenzyme Produced by Komagataella phaffii.","authors":"Nurgul Abul, Seyda Yildiz Arslan, Yagmur Unver, Hasan Ozdemir","doi":"10.1007/s12010-025-05239-8","DOIUrl":"https://doi.org/10.1007/s12010-025-05239-8","url":null,"abstract":"Water pollution is a significant issue due to industrialization and population growth, and one of the main sources of wastewater is synthetic dyes. The textile sector is particularly affected by dyes like azo and anthraquinone dyes, which are difficult to degrade and produce toxic organic waste. Currently, synthetic dyes are processed through physical and chemical methods, which have financial and methodological disadvantages. Horseradish peroxidase (HRP) is a widely studied enzyme for purifying pollutants like dyes and phenols in wastewater. However, their high cost makes them a costly option. Recombinant protein production is suitable for the mass production of stable and resistant enzymes. In this study, the decolorization potential of recombinant HRP A2A (rHRP A2A) isoenzyme secreted by Komagataella phaffii and purified by affinity technique in a single step on Acid blue 113, Alizarin red, and Remazol brilliant blue R was presented for the first time, and the optimal conditions for the highest decolorization rate were determined. Fe2+ and Mn2+ metal ions increased enzyme activity by 158.62% and 79.54%, respectively. Color removal with 0.006 EU/mL rHRP A2A for Acid blue 113, Alizarin red, and Remazol brilliant blue R was observed at 71.27, 62.26, and 31.22%, respectively. ABTS served as a redox mediator, significantly increasing the rate of dye decolorization in a shorter period at the specified concentration.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocatalytic Synthesis of N-trans-feruloyltyramine Using an Amide Bond Synthetase with an ATP Recycling. 酰胺键合成酶与ATP循环生物催化合成n -反式阿铁酰乙胺。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-02 DOI: 10.1007/s12010-025-05250-z

Bingshan Zhao, Jason Micklefield, Yonghua Wang, Fanghua Wang

{"title":"Biocatalytic Synthesis of N-trans-feruloyltyramine Using an Amide Bond Synthetase with an ATP Recycling.","authors":"Bingshan Zhao, Jason Micklefield, Yonghua Wang, Fanghua Wang","doi":"10.1007/s12010-025-05250-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05250-z","url":null,"abstract":"N-trans-feruloyltyramine (FLA) is one kind of phenylpropanoid compound found in various plants. Numerous studies have confirmed that it exhibits a wide range of physiological functions, such as antioxidant, ɑ-glucosidase inhibition, and anti-inflammatory activity. However, the low content of FLA in plants greatly limits its potential use in food and pharmaceutical industries. It is, therefore, very important to establish an effective synthesis of FLA. In this study, a green and efficient method to synthesize FLA was sought using an amide bond synthetase (ABS) biocatalyst. Ten kinds of ABS enzymes, including AlCfaL from Azospirillum lipoferum, were screened as the potential biocatalysts for the production of FLA. To obtain optimum reaction conditions, the effects of various parameters on conversion of FLA were firstly evaluated. Under the optimum conditions using 1 mM N-trans-ferulic acid, 50 mM tyramine (substrate ratio of 1:50), 10 mM MgCl₂, 8 mM ATP, and 35 µM AlCfaL enzyme at 30 °C with a shaking speed of 500 r/min for 48 h, maximum conversion rate of 74% was reached. Given that the amidation reaction is mediated by relative expensive ATP, we further optimized reaction systems to incorporate an ATP recycling system consisting of a polyphosphate kinase enzyme (CHU) and an inexpensive polyphosphate (PolyP) as the phosphate donor. Response surface methodology (RSM) based on five-level, five-variable central composite design (CCD) was used to evaluate the optimal parameters for the production of FLA. The effects of AMP, PolyP, AlCfaL, CHU concentrations, and reaction time on the conversion rate of FLA were analyzed. The optimum conditions derived via RSM were 7.12 mM AMP, 5.96 mg/mL PolyP, 39.72 μM AlCfaL, 27.68 μM CHU, and a reaction time of 36 h. Validation experiments conducted under these optimized conditions yielded an actual conversion rate of 63.5%, which compared well to the maximum predicted value of 64.2%.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untargeted Metabolomics Revealed Metabolomic Profile in Patients with Primary Systemic Sclerosis. 非靶向代谢组学揭示原发性系统性硬化症患者的代谢组学特征。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-02 DOI: 10.1007/s12010-025-05249-6

Jinling Tang, Pinglang Ruan, Zhu Wei

{"title":"Untargeted Metabolomics Revealed Metabolomic Profile in Patients with Primary Systemic Sclerosis.","authors":"Jinling Tang, Pinglang Ruan, Zhu Wei","doi":"10.1007/s12010-025-05249-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05249-6","url":null,"abstract":"Systemic sclerosis (SSc) is a systemic autoimmune disease characterized by increased morbidity and mortality. The intestinal microbiome and serum metabolites had been implicated in SSc, but the connection between the gut microbiome and serum metabolites remains poorly understood. In this study, we aimed to investigate the relationship between the gut microbiome and serum metabolome in SSc patients. Untargeted metabolomics was employed to examine the metabolic profiles of SSc patients. The results revealed significant alterations in metabolic pathways, particularly beta-alanine metabolism and pyrimidine metabolism in SSc patients. Specifically, reductions in spermine and beta-alanine were observed within beta-alanine metabolism, while uridylic acid decreased in pyrimidine metabolism. Furthermore, fecal microbiome analysis showed an increased relative abundance of Firmicutes, Verrucomicrobia, and Proteobacteria in SSc patients, whereas the abundance of Bacteroidetes and Actinobacteria was reduced at the phylum level. KEGG pathway analysis, combined with transcriptomic analysis of peripheral blood from SSc patients, identified upregulation of Toll-like receptor signaling, TNF signaling, lipid and atherosclerosis pathways, IL-17 signaling, and AMPK signaling. In summary, we performed a comprehensive analysis of the metabolic profile, which may provide insights for understanding the mechanisms of SSc.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0