Applied Biochemistry and Biotechnology最新文献

{"title":"Therapeutic Potential of Suaeda japonica Makino Leaf Extract Against Obesity in 3T3-L1 Preadipocytes and HFD-Induced C57BL/6 J Mice.","authors":"Ajithan Chandrasekaran, Yongsam Jeon, Seo-Young Kim, Dong-Hoon Seo, Heung Joo Yuk, Eunjung Son, Dong-Seon Kim, Seung-Hyung Kim, Geung-Joo Lee","doi":"10.1007/s12010-024-05170-4","DOIUrl":"https://doi.org/10.1007/s12010-024-05170-4","url":null,"abstract":"<p><p>The worldwide obesity prevalence is increasing, affecting around 4 million individuals annually. This research critically evaluated the anti-obesity efficacy of the Korean mudflat halophyte herb Suaeda japonica (Suaeda japonica Makino). In the obese mice model, the administration of 200 mg/kg b.w. of S. japonica extract (SJE) significantly mitigated obesity by modulating body and organ weight, food efficiency ratio, energy expenditure, multiple blood chemistry parameters, lipid accumulation, adipose tissue hypertrophy, and various gene expressions associated with lipogenesis and thermogenesis. The significant obesity control (80%) of the aforementioned concentration of SJE treatment in mice mimics the plant-derived commercial anti-obesity drug Garcinia cambogia (Garcinia gummi-gutta) (80%, 245 mg/kg) b.w. Since SJE has not been extensively studied for obesity management, this study demonstrated that it might influence physiological, biochemical, and molecular pathways to combat obesity and related metabolic illnesses.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-encapsulation of Creatininase, Creatinase, and Sarcosine Oxidase in Yeast Spore for Creatinine Degradation.","authors":"Jun Kong, XiaoLong Han, HuaPing Pan, MeiLing Lei, ShuQi Qi","doi":"10.1007/s12010-024-05163-3","DOIUrl":"https://doi.org/10.1007/s12010-024-05163-3","url":null,"abstract":"<p><p>Creatinine clearance is used to reflect the glomerular filtration rate to assess kidney function. Creatinine degradation-related enzymes have been used for creatinine detection in clinical medicine. The mixture of spores encapsulating either creatininase or creatinase or sarcosine oxidase could mediate a three-step reaction to produce hydrogen peroxide from creatinine. In this study, to achieve consecutive and efficient creatinine detection, degradation enzymes creatininase, creatinase, and sarcosine oxidase were co-encapsulated in a single Saccharomyces cerevisiae spore. The co-encapsulation spores performed high specific activity and enzymatic properties and converted creatinine to H₂O₂, which was 160% higher than the mixture of spores that individually expressed these three enzymes. The detection condition of co-encapsulation was optimized for the store at room temperature and resistance to environmental stresses. The S. cerevisiae spores can co-encapsulate enzyme families and catalyze consecutive reactions in the spore wall, having potential application prospects.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An In Silico Approach to Uncover Selective JAK1 Inhibitors for Breast Cancer from Life Chemicals Database.","authors":"Sruthy Sathish, Honglae Sohn, Thirumurthy Madhavan","doi":"10.1007/s12010-024-05109-9","DOIUrl":"https://doi.org/10.1007/s12010-024-05109-9","url":null,"abstract":"<p><p>JAK1, a key regulator of multiple oncogenic pathways, is a sought-out target, and its expression in immune cells and tumour-infiltrating lymphocytes (TILs) is associated with a favorable prognosis in breast cancer. JAK1 activates IL-6 via ERBB2 receptor tyrosine kinase signalling and promotes metastatic cancer and STAT3 activation in breast cancer cells. Hence, targeting JAK1 in breast cancer is being explored as a potential therapeutic strategy. A comprehensive in silico approach was utilised in this study to identify selective JAK1 inhibitors from the Life chemicals database. First, we utilised an anticancer focussed library and performed molecular docking to screen against JAK1 protein. The top 10 compounds from docking were taken for cross-docking, to assess the selectivity towards JAK1 target. Lipinski's RO5 was checked for eliminating the compounds that violate rules. Toxicity, biological activity and reactivity for the identified best compounds were predicted by Protox-II server, PASS server and cDFT analysis respectively. MD simulations were carried out to examine the stability and dynamic behaviour of the top leads, including the long-term stability of the ligand-receptor complex and any conformational changes. Lastly, the MM/PBSA method was used to determine the binding free energy of the protein-ligand complex. Our in silico approach has yielded a promising set of compounds F2638-0133, F3408-0020 and F5833-7435 with the potential to selectively target JAK1, a critical player in breast cancer progression. The docking, simulation and MM/PBSA results were compared with standard drug abrocitinib. Identified compounds exhibit favorable binding interactions, electronic properties and robust stability profiles compared to standard drug, making them promising leads for further experimental validation.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi Targeted Activity of Cocculus hirsutus through Modulation of DPP-IV and PTP-1B Leading to Enhancement of Glucose Uptake and Attenuation of Lipid Accumulation.","authors":"D Bhuvaneswari, B Riitvek, B S Lakshmi","doi":"10.1007/s12010-024-05142-8","DOIUrl":"https://doi.org/10.1007/s12010-024-05142-8","url":null,"abstract":"<p><p>Multi-targeted therapies are gaining attention in the management of multifactorial diseases due to their poly pharmacology, enhanced potency and reduced toxicity. Metabolic disorders like Type 2 diabetes mellitus (T2DM) and obesity necessitate multi-targeted therapy to improve insulin sensitivity, regulate glucose homeostasis and support weight loss. Medicinal plants rich in bioactive compounds exhibit multi-targetted action with minimal side effects. In the current study, Cocculus hirsutus methanol extract (CME) and its hydromethanolic fraction (HMF) were investigated for their multi-target potential. Significant inhibition of Dipeptidyl peptidase IV (DPP-IV), a key enzyme in glucose metabolism was observed due to CME (54%) and HMF (70%) at 10 µg/ml and 1 µg/ml respectively. Protein Tyrosine Phosphatase 1B (PTP-1B), involved in the regulation of insulin signalling, was also inhibited by CME (67%) and HMF (73%) at 10 µg/ml concentration. An increase in glucose uptake was observed due to CME (62% and 65%) and HMF (63% and 68%) in 3T3-L1 adipocytes and L6 myotubes at 100 ng/ml. Further, investigation of HMF showed a decrease in lipid accumulation by 63% at 1 µg/ml in 3T3-L1 cells. Interestingly, HMF improved insulin sensitivity by upregulating GLUT4 expression (p < 0.05) via the PI3K/AKT pathway in both 3T3-L1 adipocytes and L6 myotubes. An inhibition in lipid accumulation was also observed by suppression of Peroxisome proliferator-activated receptor γ (PPARγ) (p < 0.05), a key regulator of adipogenesis in 3T3-L1 adipocytes. Gas chromatography-mass spectrometry analysis of the HMF showed the major component to be 3-methylmannoside (26.52%).</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin Derivatives from Bidens pilosa Suppressed Cell Proliferation via Inhibition of RSK2 Kinase and Aldose Reductase Enzymes: UPLC-MS/MS, GC-MS, In Vitro, and Computational Studies.","authors":"Doaa S Ali, Alaadin E El-Haddad, Hussein S Mohamed, Ashraf A El-Bassuony, Momtaz M Hegab, Gehad AbdElgayed, Hossam Ebaid, Shimaa A Ahmed, Emadeldin M Kamel","doi":"10.1007/s12010-024-05134-8","DOIUrl":"https://doi.org/10.1007/s12010-024-05134-8","url":null,"abstract":"<p><p>Traditionally, Bidens pilosa L. is an edible herb utilized for various ailments. The study accomplished a complete analysis of B. pilosa extract including UPLC/T-TOF-MS/MS, GC-MS, and in vitro antiproliferative activity, in addition to molecular docking on kinase and aldose reductase enzymes. From GC-MS analysis, the percentage of identified unsaturated fatty acids (FAs) (11.38%) was greater than saturated FAs (8.69%), while the sterols percent (39.92%) was higher than the hydrocarbons percent (6.6%). Oleic and palmitic acids are the major FAs (9.48% and 6.14%, respectively). Phytochemical profile uncovered the presence of quercetin, kaempferol, myricetin, and isorhamnetin aglycones and/or glycoside derivatives alongside apigenin, acacetin, and luteolin derivatives. B. pilosa extract suppressed cell proliferation in a concentration-dependent manner against SNB-19 and SK-MEL-5 cell lines (IC₅₀ 1.66 ± 0.06 and 4.04 ± 0.14 mg/mL, respectively). These potentials aligned with the molecular docking results on aldose reductase and kinase enzymes with promising binding affinities (- 5.3 to - 8.89 kcal mol^-1). B. pilosa metabolites were found as kinases and aldose reductase inhibitors, which rationalize their antiproliferative activity. Unfortunately, toxicity assessments were not performed to assess the safety of B. pilosa extract. Assessment of the therapeutic efficiency via in vivo and clinical studies is required.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation and Characterization of Eco-Technological Synthesis of Spherical TiO₂ Nanoparticles from Thalassia hemprichi and Analysis of Biomedical Properties.","authors":"Velmani Sundar, Silambarasan Tamil Selvan, Arularasu M V, Maruthupandian Arumugam, Santhosh Chinnaraj","doi":"10.1007/s12010-024-05143-7","DOIUrl":"https://doi.org/10.1007/s12010-024-05143-7","url":null,"abstract":"<p><p>In this present investigation, plant-mediated synthesis of titanium oxide (TiO₂) nanoparticles was synthesized from seagrass (Thalassia hemprichi) using the hot plate combustion method (HPCM). Synthesized TiO₂ nanoparticles optical, functional, structural, and morphology properties were analyzed by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). SEM analysis confirmed the spherical shape of the TiO₂ nanoparticles were observed in various sizes, viz., 50 nm and 78 nm. The XRD analysis revealed that TiO₂ nanoparticles have a body-centred cubic structure without a secondary phase. Green synthesized TiO₂ nanoparticle applications were studied against the antimicrobial, antioxidant, anticancer, and photocatalytic activity. The pathogenic bacterial strains, including Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumonia, and Pseudomonas aeruginosa, were tested against TiO₂ nanoparticles; the maximum level of activity was seen at a concentration of 50 µg/mL. The antioxidant assays were performed against TiO₂ nanoparticles, and inhibitory concentration values (IC₅₀) were 40.28 μg/mL of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 52.04 µg/mL of the acrylamide tertiary butyl sulfonic acid (ATBS) assay, and 16.91 µg/mL of the metal chelating assay. The anticancer activity was analyzed against MCF-7 cancer cells using TiO₂ nanoparticles, and the IC₅₀ value showed 64.14 µg/mL concentration. An eco-friendly and convenient method was formulated for the production of titanium oxide nanoparticles utilizing seagrass extract. The potential employment of TiO₂ involves water treatment, biomedicine, biosensors, and nanotechnology.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and Genomic Potential of Burkholderia contaminans PB_AQ24 Isolate for Boosting the Growth of Bamboo Seedlings in Heavy Metal Contaminated Soils.","authors":"Poonam Bhanse, Lal Singh, Asifa Qureshi","doi":"10.1007/s12010-024-05156-2","DOIUrl":"https://doi.org/10.1007/s12010-024-05156-2","url":null,"abstract":"<p><p>The present study investigated the genomic and functional potential of Burkholderia contaminans PB_AQ24, a bacterial strain isolated from the municipal solid waste dumpsite, for boosting the growth of Dendrocalamus strictus (Male bamboo) seedlings. The isolated strain exhibited high potency for metal solubilization and ACC (1-Aminocyclopropane-1-carboxylate) deaminase activity. Its genome harbored diverse genes responsible for nitrogen and phosphorus utilization (trpABCDES, iaaH, acdS, pstABCS, phoAUD, pqqABCDE, kdpABC, gln, and nirBD) and also an abundance of heavy metal tolerant genes (ftsH, hptX, iscX-fdx-hscAB-iscAUR, mgtA, corA, and copC). Seeds priming experiments carried out in heavy metal contaminated soils (such as waste dumpsite soil (WDS), fly ash dumpsite soil (FAS) and natural garden soil (NGS control)) augmented with Burkholderia contaminans sp. PB_AQ24 showed 85% sustenance of seedlings in WDS and 80% in FAS. The study thus highlighted the potential features in isolated bacterial strain Burkholderia sp. PB_AQ24 (NCBI accession no. JAQOUG000000000), which could boost the growth of bamboo seedlings in heavy metal contaminated soils and may be applied for restoration and rejuvenation of contaminated sites.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quinoline: A Novel Solution for Next-Generation Pesticides, Herbicides, and Fertilizers.","authors":"Shahid Ullah Khan, Taufiq Nawaz, Osama Alam, Dilfaraz Khan, Shah Fahad, Shah Saud, Kun Lu","doi":"10.1007/s12010-024-05164-2","DOIUrl":"https://doi.org/10.1007/s12010-024-05164-2","url":null,"abstract":"<p><p>Quinoline is a nitrogen-containing heterocycle compound widely used in the medical industry for its pharmacological properties, such as its antimalarial, antimicrobial, antiparasitic, anti-inflammatory, and anticancer activities. Beyond its medical significance, quinoline shows promising applications in agriculture as a safe and effective pesticide, herbicide, and fertilizer. This review explores the evolution of quinoline research, beginning with its history and synthesis and transitioning to its biological activities and their relevance in agriculture. It then highlights the potential applications of quinoline in modern agriculture, such as pesticides, herbicides, and fertilizers, for increasing crop yields and resilience while reducing crop waste. Moreover, it discusses formulation strategies that can enhance the efficacy of quinoline. Finally, the review addresses potential challenges, such as toxicity and environmental impact, underscoring the need for further research to harness quinoline's full potential in sustainable agriculture.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Benzo[d]thiazol-2-Amine Derivatives, Synthesis, and Molecular Docking Insights Potential Anticancer Agents Targeting HER Enzyme and DNA.","authors":"Ammar M K Al-Azzawi, Ekhlas Abdallah Hassan","doi":"10.1007/s12010-024-05149-1","DOIUrl":"https://doi.org/10.1007/s12010-024-05149-1","url":null,"abstract":"<p><p>The synthesis and characterization of benzo[d]thiazol-2-amine derivatives, which were prepared by reacting benzothiazole with para-aminobenzophenone in ethanol, supplemented with glacial acetic acid. Subsequently, compound (2) was synthesized from compound (1) using NaNO₂, H₃PO₄, and HNO₃ in a water-based solvent, resulting in 2-hydroxy-1-naphthaldehyde. Another derivative, compound (3), was synthesized by reacting compound (1) with vanillin under similar conditions. Structural characterization involved IR spectroscopy and melting point determination, while molecular properties were estimated to assess drug-like characteristics. The main point of this study is to synthesize and research drug-like characteristics, biological activities, and docking studies. Molecular docking studies (MDS) were conducted using AutoDock Vina to evaluate the binding affinity of compounds 1, 2, and 3 with the enzyme Human Epidermal growth factor receptor (HER). The docking simulations aimed to elucidate drug-DNA interactions, focusing on hydrogen bonding, hydrophobic interactions, and binding energies. The compounds' conformations were analyzed to identify their potential binding modes within the DNA groove. Compounds 2 and 3 exhibited higher binding affinities to the HER enzyme compared to compound 1, with compound 2 showing the highest affinity docking scores of - 10.4, - 9.9, and - 9.8 kcal/mol for the top three poses. These results suggest that compounds 2 and 3 could potentially interact more effectively with the enzyme and DNA, attributed to their structural features and interaction profiles. Synthesized and characterized benzo[d]thiazol-2-amine derivatives and evaluated their biological activities against gram-positive and gram-negative bacteria. The compounds demonstrated diverse biological activities, likely due to the various functional groups within their 4- to 5-ring structures. Molecular docking studies indicated that compounds 2 and 3 have promising potential as cancer therapy candidates, showing strong binding affinities to the HER enzyme and effective interactions with DNA.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly Synthesis of Iron Oxide Nanoparticles Using Parietaria alsinifolia Extracts and Evaluation of Biological Applications.","authors":"Zakir Ullah, Javed Iqbal, Banzeer Ahsan Abbasi, Farhat Gul, Sarfaraz Ali, Sobia Kanwal, Reem M Aljowaie, Ghulam Murtaza, Rashid Iqbal, Tariq Mahmood","doi":"10.1007/s12010-024-05151-7","DOIUrl":"https://doi.org/10.1007/s12010-024-05151-7","url":null,"abstract":"<p><p>The current research was conducted to synthesize Parietaria alsinifolia-mediated iron oxide nanoparticles (P.A@FeONPs) using the green and eco-friendly protocol. The biosynthesized P.A@FeONPs were characterized using various approaches like UVs, FTIR, SEM, EDX, and DLS. The mean crystallite size was calculated to be ~ 21.48 nm using the Debye-Scherrer equation. Further, various in vitro biological assays were performed to analyze the therapeutic potentials of FeONPs. 2,2-Diphenyl-1-picrylhydrazy (DPPH) antioxidant activity was performed to reveal the DPPH radical scavenging potential of P.A@FeONPs and was calculated as 72%. Similarly, the total reducing power was determined as 65.45 ± 1.77%. In addition, P.A@FeONPs exhibited a significant total antioxidant capacity of 87 ± 4.8%. Antibacterial and antifungal assays were performed using the disc diffusion method. Among the different bacterial strains accession (EFB-10-2023 M.B), Rhodococcus jostii has shown the highest zone of inhibition (23.9 mm at 1000 μg/mL), while Escherichia coli displayed a 22.65 mm zone of inhibition at (1000 μg/mL). Similarly, Aspergillus niger exhibited a substantial zone of inhibition (28.75 mm). A brine shrimp cytotoxicity assay revealed the cytotoxicity potential (LC₅₀ 244.92 μg/mL). P.A@FeONPs were also tested against red blood cells, HEK-293, and VERO cell lines (< 200 μg/mL) to validate their biocompatibility. An alpha-amylase inhibition assay demonstrated 68.66% inhibition and substantial cytotoxicity against Hep-2 liver cancer cells (IC₅₀ 100 μg/mL). In conclusion, P.A@FeONPs have shown significant bioactivities. In the future, we recommend other biological and catalytic activities using different animal models to explore its potential further.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}