Applied Biochemistry and Biotechnology最新文献

{"title":"Geo-Referencing Biochemical Diversity for Conservation Prioritization in Jackfruit (Artocarpus heterophyllus Lam.).","authors":"Suresh Chandra Gurjar, Shashi Bhushan Choudhary, Vijai Pal Bhadana, Hariom Kumar Sharma, Keshav Kumar Jha, Bikash Das, Mahesh Kumar Dhakar, Gyanendra Pratap Singh","doi":"10.1007/s12010-025-05420-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05420-z","url":null,"abstract":"<p><p>Eighty jackfruit genotypes from Eastern India were evaluated for biochemical traits, including total soluble sugar, acidity, sugar types, phenols, and antioxidant activities. High coefficients of variation (15.97-38.37%) and heritability (0.72-0.96) were observed. Geospatial analysis identified Simdega, Jharkhand, as a hotspot for genotypes with superior nutritional traits. Cluster analysis revealed 17 elite fruit-type genotypes. SSR marker-trait association explained significant phenotypic variation, particularly for pulp weight and antioxidant content. These findings support conservation and breeding strategies for jackfruit improvement.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS Profiling and In Silico Pathway Mapping of Brevibacterium casei VRK 1 Metabolites for exploring Anti-inflammatory and Antioxidant Activities.","authors":"Kavitha Rangasamy, Vishnupriya Chandrasekaran, Mohamedrizwan Kadersulthan, Gowtham Murugesan, Jeyakumar Natarajan","doi":"10.1007/s12010-025-05400-3","DOIUrl":"https://doi.org/10.1007/s12010-025-05400-3","url":null,"abstract":"<p><p>The emergence of new metabolites from marine actinobacteria for disease treatment has increased significantly. In this context, the study focused on isolating and characterizing a marine actinobacterium from Ervadi Beach in the Ramanathapuram district. The isolated strain was identified by proteomic fingerprinting and 16S rRNA gene analysis. The ethyl acetate crude extract of Brevibacterium casei VRK 1 was tested for its antioxidant and anti-inflammatory properties. GC-MS analysis was used to identify the bioactive metabolites. The classification of bioactive metabolites, their functions, and their metabolic pathways was screened using an in silico approach. Based on the proteomic fingerprinting and sequencing results, the strain was identified as Brevibacterium casei VRK1. Notable anti-inflammatory activity was observed at 500 µg/mL, with an inhibition percentage of 35.76%. Strong antioxidant activity was also detected for DPPH and ABTS at 500 µg/mL, with inhibition percentages of 45.9% and 40.12%, respectively. GC-MS analysis confirmed the presence of ten novel bioactive metabolites with the highest probability values. The bioactive metabolites of B. casei VRK were identified as organometalloid compounds, fatty acyls, steroids, carboxylic acids, and their derivatives. The metabolomics results highlighted the biological functions of B. casei VRK1, including antidepressant, carboxylesterase, and dihydrofolate reductase inhibition. Pathway analysis results revealed that metabolites were associated with the biosynthesis of steroid hormones and fatty acids. This provides valuable insights into in silico methods for identifying the importance of secondary metabolites with anti-inflammatory and antioxidant effects. The KEGG pathway analysis results offer initial evidence of secondary metabolites and their related pathways.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Industrial Production of PEGylated L-asparaginase: Process Design and Techno-economic Assessment Through Simulation.","authors":"Adamo Ferreira, Nicola Sgrignoli Fuganholi, Marina S R Silva, Karin Mariana Torres-Obreque, Rafael G Ferreira, Adalberto Pessoa-Jr, Adriano Rodrigues Azzoni","doi":"10.1007/s12010-025-05388-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05388-w","url":null,"abstract":"<p><p>L-asparaginase is a biopharmaceutical enzyme used as a first-line treatment for acute lymphoblastic leukemia (ALL). Despite its clinical relevance, only a limited number of microbially produced L-asparaginase formulations are commercially available. Among these, PEGylated versions are preferred due to their reduced dosing frequency and lower incidence of side effects. However, PEGylated enzymes are significantly more expensive to produce, and techno-economic evaluations of such products remain scarce in the literature. This study presents a conceptual process design and a techno-economic analysis, conducted through simulation, for the production of PEGylated L-asparaginase. Based on the scale-up of process parameters developed by our research group, multiple production scenarios were evaluated. The simulation of different fermentation modes and final biomass concentration revealed key cost drivers, including biomass yield, PEGylation reagent cost, and the efficiency of unreacted enzyme removal during purification. In the best-performing scenario, which employed fed-batch fermentation with 40 g/L of biomass, the unit production cost (UPC) of L-asparaginase was estimated at USD 5.81 per mg. Among all factors, plant depreciation and the cost of mPEG-NHS were identified as the most influential factors on the UPC. The findings support the feasibility of developing small-scale biopharmaceutical production facilities aimed at enhancing local self-sufficiency and reducing vulnerability to global supply chain disruptions.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic Synthesis and Nematicidal Potential of Silver Nanoparticles Derived from Indigenous Microbes of Vietnam's Central Highlands.","authors":"Van Chung Do, Tu Quy Phan, Van Bon Nguyen","doi":"10.1007/s12010-025-05413-y","DOIUrl":"https://doi.org/10.1007/s12010-025-05413-y","url":null,"abstract":"<p><p>Green silver nanoparticles (AgNPs) biosynthesis via microbe-mediated processes has many advantages, attracting much attention in research. In this study, the biosynthesis of green AgNPs using cell-free fermented broth (CFFB) of native bacterial strains in the Vietnam Central Highlands jungle was first exploited. After screening more than 60 strains, CFFB from strain LV-V3 showed the most effective AgNPs biosynthesis ability, with AgNPs synthesis efficiency of 76.7809%. This potential strain was identified as Pantoea coffeiphila via 16S rRNA gene sequence identification. The most suitable condition for green AgNPs biosynthesis was determined at a AgNO₃/CFFB ratio of 3:1, a CFFB dilution of 1:20, 1.5 mM AgNO₃, 70 °C, pH 6, and a 48-h reaction time. AgNPs characteristics were evaluated via UV-Vis, SEM, TEM, XRD, and FTIR analysis. The obtained AgNPs are spherical and small in average size (8.5 nm). Furthermore, their stability was examined over 3 months, showing high stability with their shape, UV-Vis absorption, and zeta potential, with no significant change. The novel bioactivity of these green AgNPs was evaluated against the black pepper nematode. The inhibition of both egg-hatching and second-stage juveniles of AgNPs was much better than that of CFFB at all tested concentrations. Evidence of the penetration ability of AgNPs biosynthesized from P. coffeiphila LV-V3 fermented broth into eggs and juveniles, involved in its strong anti-nematode effect, was also elucidated. This research indicated green AgNPs from a novel microbial source from the Vietnam Central Highlands jungle for effectively managing plant-parasitic nematodes.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino Acid Ionic Liquid Activation of Biomimetic Magnetic Graphene for Enhancing Lipase Stability and Catalytic Performance.","authors":"Junfeng Wang, Yongjin Sheng, Suomin Wang, Can Li, Kai Shen, Zhenfang Liu, Futong Liu, Siyu Tian, Yanmei Zhou, Tianyi Huang, Wei Zhang","doi":"10.1007/s12010-025-05393-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05393-z","url":null,"abstract":"<p><p>The limited stability and insufficient recyclability of lipase (CRL), a crucial biocatalyst, hinder its extensive industrial application. In this study, we successfully immobilized CRL on a magnetic graphene surface by using polyethyleneimine and polydopamine (PEI/PDA) bionic adhesive as a flexible connecting branch and amino acid ionic liquid as an activator. The immobilized enzyme exhibited markedly enhanced thermal stability and denaturant tolerance. Specifically, the immobilized enzyme retained more than 80% activity after incubation at 60 °C for 1 h, which was approximately 1.8 times higher than that of the free enzyme. Additionally, following incubation in 6 mol/L denaturant for 2 h, the immobilized enzyme maintained over 80% activity. Moreover, the immobilized enzyme displayed superior storage stability and magnetic properties, significantly simplifying the recovery process. Notably, even after seven reuses, the immobilized enzyme retained more than 70% of its catalytic activity. When the immobilized enzyme was applied to the synthesis of phytosterol esters, an esterification rate of 90.2% was achieved. Moreover, the enzyme maintained a high activity level, with the esterification rate still exceeding 70% after five cycles of reuse, demonstrating excellent reusability. This research would not only offer innovative strategies for lipase immobilization but also establish a robust theoretical framework for the application of ionic liquids in immobilized enzyme technology, thereby providing important support for the industrial application of biocatalysts.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory Effects of Hesperetin and EGCG in the Lung Cancer Progression involves impairment in TOP2A gene expression regulation.","authors":"Rangaraj Kaviyaprabha, Thandaserry Vasudevan Miji, Unni Apsara, Sekar Sindhurani, Rajalakshmi Sabanayagam, Sridhar Muthusami, Muruganantham Bharathi","doi":"10.1007/s12010-025-05426-7","DOIUrl":"https://doi.org/10.1007/s12010-025-05426-7","url":null,"abstract":"<p><p>Lung cancer (LC) remains the leading cause of death among all cancer-related mortalities. Despite advances in Non-Small Cell Lung Cancer (NSCLC) treatment, rising drug resistance necessitates the development of new therapeutics targeting relevant biomarkers. Understanding resistance mechanisms is key to improving treatment effectiveness and overcoming drug resistance. This study aims to identify crucial biomarkers and evaluate potential drug-like compounds targeting them using both in silico and in vitro analyses. Ten key biomarkers associated with LC-including Assembly Factor for Spindle Microtubules (ASPM), Budding Uninhibited by Benzimidazoles 1 (BUB1), Centromere Protein F (CENPF), DNA Topoisomerase II Alpha (TOP2A), TPX2 Microtubule Nucleation Factor (TPX2), Kinesin Family Member 15 (KIF15), Ubiquitin Conjugating Enzyme E2 C (UBE2C), Marker of Proliferation Ki-67 (MK167), Hyaluronan-Mediated Motility Receptor (HMMR), and Cyclin B2 (CCNB2)-were identified from GEO datasets (GSE4882, GSE40275, GSE22874, GSE166720, GSE7880, GSE1987, GSE45142, GSE162102, GSE63571, and GSE23361) using the GEO2R tool. A molecular docking study involving 18 natural compounds was performed against these top 10 biomarker proteins. The compounds hesperetin (binding affinity [BA] = -10.5 kcal/mol; root mean square deviation [RMSD] = 1.569 Å) and epigallocatechin gallate (EGCG) (BA = -9.4 kcal/mol; RMSD = 0.025 Å) showed strong interactions with the TOP2A protein. To substantiate these findings, the efficacy of hesperetin and EGCG was tested in LC cells. The combination treatment had an IC₅₀ of 78 µg/mL in A549 LC cells and significantly reduced cell proliferation, migration, and TOP2A gene expression, as assessed by MTT assay, wound healing assay, and RT-PCR. The results corroborate the computational predictions and support the utility of EGCG and hesperetin in targeting LC at the cellular level.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breaking Boundaries in Cell Culture: The Development and Impact of Serum-Free Media.","authors":"Yonghong Huang, Zhiming Hu, Yijie Liu, Huizhen Li, Jia Zhao, Peng Yu","doi":"10.1007/s12010-025-05391-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05391-1","url":null,"abstract":"<p><p>With the advancement of cell culture technology, the development of serum-free media (SFM) has become an important branch of modern biomedical research. This paper reviews the evolution from traditional fetal bovine serum (FBS)-dependent media to animal-derived (NAO)-free, protein-free (PFM), and chemically defined (CDM) media. These SFM not only address ethical and safety concerns in the use of FBS but also improve experimental reproducibility and reduce the risk of biological contamination. We highlight the applications of SFM in three major areas: cell therapy, gene therapy, and the production of vaccines and biological products. In these fields, SFM enables safer, scalable, and GMP-compliant manufacturing of cells, viral vectors, and recombinant proteins. Finally, we discuss future directions in medium development, emphasizing fully defined, customizable formulations to meet clinical and industrial needs. With this article, we provide a new perspective for researchers and developers to optimize the formulation of serum-free culture media to facilitate their widespread adoption in biomedical research and applications.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling and Optimization of Ultrasound-Assisted Extraction of Antioxidants from Yerba Mate (Ilex paraguariensis St. Hill) Using Artificial Neural Networks and ANFIS.","authors":"Alini Rafaela Neitzki Peters, Fabio Fiorin Cardoso Filho, Jéssyca Ketterine Carvalho, Edson Antonio da Silva, Emmanuel Zullo Godinho, Fernando de Lima Caneppele, Camila da Silva, Salah Din Mahmud Hasan","doi":"10.1007/s12010-025-05423-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05423-w","url":null,"abstract":"<p><p>The leaves of yerba mate, commonly found in the southern region of Brazil, are consumed as teas and contain a variety of antioxidant compounds with nutritional and pharmacological value. This study aimed to modeling and optimizing the extraction process of these compounds using ultrasound, where operational parameters such as temperature (T), ultrasound power (P), and the solvent-to-solid ratio of yerba mate (R) were evaluated, and four responses measured, including extraction yield, total phenolic content (TPC), and antioxidant capacity determined by the FRAP and DPPH methods. The Box-Behnken design (BBD) was initially used to optimize the variables and generate a response surface model. Subsequently, artificial neural networks (ANN) and adaptive neuro-fuzzy inference system (ANFIS), were applied to enhance prediction and optimization of the results. The findings indicated that ultrasound-assisted extraction (UAE) achieved a TPC of approximately 500 mg GAE g⁻¹ and a DPPH activity of ~ 480 μmol Trolox g⁻¹ under optimal conditions. Statistical modeling using BBD allowed for the identification of optimal operational conditions to maximize the studied responses simultaneously. Additionally, the ANN and ANFIS models demonstrated excellent predictive performance, with high coefficients of determination (R² > 0.99) and low prediction errors (RMSE). The ANFIS method proved particularly effective, accurately predicting yields of antioxidants with R² values of 0.993, 0.998, and 0.994 for Yield, TPC, and DPPH, respectively, along with low RMSE values, corroborating its effectiveness. In conclusion, machine learning tools such as ANN and ANFIS are robust and precise alternatives for modeling UAE as well as other complex processes involving the manipulation and optimization of process variables.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Biodiversity to Breakthroughs: The Promise of Saudi Arabian Medicinal Plants in Antiviral Research.","authors":"Hamdoon A Mohammed, Riaz A Khan","doi":"10.1007/s12010-025-05431-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05431-w","url":null,"abstract":"","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving Nutritional Quality of Peanut Meal through Solid-State Fermentation with Heyndrickxia coagulans.","authors":"Hao Huang, Dan Li, Wendi Yu, Youwei Chen, Weiwei Dong, Xiang Yu, Shumiao Zhao, Yunxiang Liang, Lizhong Chen, Yuanliang Hu, Nan Peng","doi":"10.1007/s12010-025-05410-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05410-1","url":null,"abstract":"<p><p>With the increasing global demand for protein sources to feed the ever-growing population, peanut meal-a by-product of peanut oil extraction-holds promise as a valuable feed ingredient for animal protein production. However, its utilization is constrained by anti-nutritional factors and limited digestibility. This study employed solid-state fermentation using Heyndrickxia coagulans (formerly Bacillus coagulans) to enhance the nutritional quality of peanut meal. Among three evaluated strains, Heyndrickxia coagulans S17 was selected for its superior fermentation performance. Optimal fermentation conditions were established: 1 g/L of CaCl₂, 45 °C, material-to-liquid ratio of 1:2.1, 14% inoculation, and 36-h fermentation. Under these conditions, crude protein, small peptides, and acid-soluble protein contents increased from 53.75%, 3.35%, and 1.80% to 60.11%, 8.93%, and 5.37%, respectively. In vitro protein digestibility improved from 54.18% to 64.91%. Additionally, aflatoxin B 1 levels decreased from 43.87 μg/kg to 9.23 μg/kg, while trypsin inhibitors and phytic acid were reduced from 2.97 mg/g to 0.56 mg/g and 14.36 g/kg to 2.57 g/kg, respectively. These findings suggest that solid-state fermentation with Heyndrickxia coagulans S17 effectively enhances the nutritional quality of peanut meal, offering a viable approach for producing high-quality protein feed and contributing to sustainable livestock nutrition.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}