Applied Biochemistry and Biotechnology最新文献_第2页

Nanostructured Benzoic Acid-Chitosan Nanogel Encapsulating a Synergistic Blend of Seven Essential Oils for Precision Pharmacological Delivery. 纳米结构苯甲酸-壳聚糖纳米凝胶包封7种精油的协同混合物，用于精确的药物递送。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-09-06 DOI: 10.1007/s12010-025-05381-3

Neha Sharma, Zahid Nabi Sheikh, Sanjay Guleria, Parshant Bakshi, Alanoud T Alfagham, Wasmiah Saud Alotaibi, Saud Alamri, Kamran Ashraf

{"title":"Nanostructured Benzoic Acid-Chitosan Nanogel Encapsulating a Synergistic Blend of Seven Essential Oils for Precision Pharmacological Delivery.","authors":"Neha Sharma, Zahid Nabi Sheikh, Sanjay Guleria, Parshant Bakshi, Alanoud T Alfagham, Wasmiah Saud Alotaibi, Saud Alamri, Kamran Ashraf","doi":"10.1007/s12010-025-05381-3","DOIUrl":"https://doi.org/10.1007/s12010-025-05381-3","url":null,"abstract":"To develop and characterize a chemically modified chitosan-benzoic acid (CS-BA) nanogel for enhanced encapsulation, stability, and controlled release of essential oils with improved antimicrobial and antioxidant properties, the CS-BA nanogel was synthesized via carbodiimide-mediated coupling (EDC) of chitosan with benzoic acid. The nanogel was characterized using scanning electron microscopy (SEM) for morphology and Fourier transform infrared (FTIR) spectroscopy for chemical bonding analysis. The encapsulation efficiency of essential oils-Pinus roxburghii (PREO), Pinus wallichiana (PWEO), Cedrus deodara (CDEO), Thuja orientalis (TOEO), Cymbopogon citratus (CCEO), Cymbopogon khasianus (CKEO), and Mentha arvensis (MAEO) was evaluated, and in vitro release studies were conducted over 36 h. The CS-BA nanogel formed uniform spherical nanostructures (~ 1 µm diameter). FTIR confirmed successful covalent coupling via amide linkages. The encapsulation efficiency of the essential oils ranged from 79.70 ± 0.21% to 96.52 ± 0.14%. The nanogel provided sustained and controlled release of essential oils, reducing volatility and enhancing stability. The encapsulated oils exhibited significantly higher antimicrobial and antioxidant activities, maintaining prolonged functional efficacy at lower concentrations. The CS-BA nanogel serves as an efficient delivery system for essential oils, addressing stability and bioavailability challenges. This system holds promise for applications in food preservation, pharmaceuticals, and cosmeceuticals, providing a sustainable approach to enhance the functional properties of bioactive compounds.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-19b-3p Affects the Proliferation and Apoptosis of Bladder Cancer Cells by Regulating the Stability of lncRNA SNHG20. miR-19b-3p通过调控lncRNA SNHG20的稳定性影响膀胱癌细胞的增殖和凋亡

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-09-06 DOI: 10.1007/s12010-025-05370-6

Hongshuang Dai, Yunzhu Zhang, Siwen Yu, Yue Feng, Zhongjie Qiao

{"title":"miR-19b-3p Affects the Proliferation and Apoptosis of Bladder Cancer Cells by Regulating the Stability of lncRNA SNHG20.","authors":"Hongshuang Dai, Yunzhu Zhang, Siwen Yu, Yue Feng, Zhongjie Qiao","doi":"10.1007/s12010-025-05370-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05370-6","url":null,"abstract":"This study explores the mechanism of miR-19b-3p in bladder cancer (BCa) cell proliferation and apoptosis to provide the latest theoretical basis for miR-19b-3p to become a novel biomarker and therapeutic target for BCa. miR-19b-3p, lncRNA SNHG20, and HS3ST3B1 expressions in BCa tissues or cells were detected via RT-qPCR or Western blot. Cell proliferation was evaluated via CCK-8 and colony formation assays. Cell apoptosis was assessed via flow cytometry, and apoptosis related factors Bax and Bcl-2 were detected via Western blot. Dual luciferase and RIP assays confirmed the binding of miR-19b-3p and lncRNA SNHG20. The binding between lncRNA SNHG20, TARDBP, and HS3ST3B1 was analyzed by RIP, RNA pull down, and co-immunoprecipitation. The RNA stability of lncRNA SNHG20 and HS3ST3B1 was tested after actinomycin D treatment. A nude mouse xenograft tumor model was established to validate the effect of miR-19b-3p on BCa in vivo. miR-19b-3p was weakly expressed in BCa, while lncRNA SNHG20 and HS3ST3B1 were highly expressed. Overexpression of miR-19b-3p repressed BCa cell proliferation but facilitated apoptosis. Mechanistically, miR-19b-3p decreased lncRNA SNHG20 expression by binding to lncRNA SNHG20 and reducing its stability, thus repressing the interaction between lncRNA SNHG20-TARDBP-HS3ST3B1. Further in vivo experiments also revealed that miR-19b-3p restrained the in vivo tumorigenicity of BCa cells and promoted apoptosis by suppressing the lncRNA SNHG20/HS3ST3B1 axis. In conclusion, overexpression of miR-19b-3p represses BCa cell proliferation and promotes apoptosis by suppressing the lncRNA SNHG20/HS3ST3B1 axis.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Biopesticides for the Pest Control Through Inhibition of the Acetylcholinesterase Purified from Loxostege sticticalis (L.) (Lepidoptera: Crambidae). 生物农药对蝗虫乙酰胆碱酯酶抑制作用的研究(鳞翅目:Crambidae)。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-09-03 DOI: 10.1007/s12010-025-05363-5

Demet Kızıl

{"title":"Investigation of Biopesticides for the Pest Control Through Inhibition of the Acetylcholinesterase Purified from Loxostege sticticalis (L.) (Lepidoptera: Crambidae).","authors":"Demet Kızıl","doi":"10.1007/s12010-025-05363-5","DOIUrl":"10.1007/s12010-025-05363-5","url":null,"abstract":"Acetylcholinesterase (AChE) is a cholinergic enzyme called acetylcholine acetylhydrolase. Acetylcholinesterase inhibition is one of the prominent methods in studies on pest control management. In this study, AChE was purified 103.7-fold from Loxostege sticticalis (L.) using an Edrophonium-Sepharose 6B affinity column, and some kinetic properties were investigated. The molecular weight of the purified enzyme was determined by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Inhibition studies of AChE were realized with tacrine, edrophonium chloride, and cypermethrin, which are known inhibitors of AChE, as well as aqueous extracts of certain plant leaves. The subunit molecular weight of purified AChE was estimated to be approximately 56 kDa by SDS-PAGE analysis. The maximum activity of Loxostege sticticalis AChE was specified at 40.0 °C and pH 8.0. Vmax, Km, and kcat values of the AChE were determined as 243.9 ± 15.7 EU/mg protein, 0.122 ± 0.06 mM, and 13.7 ± 2.7 min-1, respectively. In the inhibition studies, the IC50 values of tacrine, edrophonium chloride and cypermethrin were found to be 3.4 ± 0.5, 0.08 ± 0.006 and 85.0 ± 4.1 μM, respectively. Besides, the IC50 values of aqueous extracts of olive leaf, alder leaf, walnut leaf, cherry laurel leaf, and plane leaf were as 12.0 ± 0.6, 15.2± 1.3, 32.1 ± 1.5, 58.3 ± 1.9, and 75.2 ± 2.8 µg dry matter/mL, respectively. In addition, the oleuropein and phenolic substance amounts of these plant extracts were determined and correlated with the IC50 values. Consequently, the plant extracts used in this study may be recommended as an alternative biopesticide source to control such pests via AChE inhibition.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoflowers: Smart Molecules for Biofilm Management. 纳米花：生物膜管理的智能分子。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-09-03 DOI: 10.1007/s12010-025-05361-7

Tahayya Haq, Samriddhi Jaswani, Roohi Roohi

{"title":"Nanoflowers: Smart Molecules for Biofilm Management.","authors":"Tahayya Haq, Samriddhi Jaswani, Roohi Roohi","doi":"10.1007/s12010-025-05361-7","DOIUrl":"10.1007/s12010-025-05361-7","url":null,"abstract":"Biofilm formation poses challenges across various sectors, such as healthcare facilities, food safety, and in industrial processes, owing to the resilience of microbial communities encased in protective extracellular matrices. This paper delves into the strategies for biofilm control, highlighting recent/novel chemical, biological, and nanotechnological approaches. Chemical methods exploit the potential of natural compounds, such as phenolic antioxidants and nanoparticles, to disrupt biofilms, thereby boosting the effectiveness of antimicrobial treatments. In parallel, biological strategies involve the use of predatory bacteria and biosurfactants and offer eco-friendly alternatives. In nanotechnology, particularly the nanoflowers, due to their unique morphology and high surface area, show greater promise in efficiently targeting biofilms by enabling enhanced bacterial interaction and precise drug delivery. The implications of these approaches are far-reaching, extending to the food industry, where biofilm formation can result in spoilage and foodborne illnesses, as well as in biomedical applications aimed at preventing infections related to medical devices. This paper underscores the potential of integrating these approaches to develop more effective and sustainable solutions for biofilm management, contributing to enhanced health and safety across multiple domains.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ORMDL2 Promotes the Growth of Glioma through mTORC1-Mediated Fatty Acid Metabolism. ORMDL2通过mtorc1介导的脂肪酸代谢促进胶质瘤的生长。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-09-02 DOI: 10.1007/s12010-025-05362-6

Xinting Lv, Chunping Li, Zhaohao Wang, Kun Xue

{"title":"ORMDL2 Promotes the Growth of Glioma through mTORC1-Mediated Fatty Acid Metabolism.","authors":"Xinting Lv, Chunping Li, Zhaohao Wang, Kun Xue","doi":"10.1007/s12010-025-05362-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05362-6","url":null,"abstract":"Fatty acid metabolism is critical for numerous biological processes involved in the pathogenesis of glioma. This study aimed to survey the role of ORMDL sphingolipid biosynthesis regulator 2 (ORMDL2) in fatty acid metabolism of glioma cells. The expression of ORMDL2 in glioma and its association with survival rate was detected using The Cancer Genome Atlas (TCGA) data and Kaplan-Meier survival curve. The effect of ORMDL2 on glioma was explored by culturing human glioma cells in vitro and constructing mouse glioma models. Cell proliferation, apoptosis, and fatty acid metabolism were detected by CCK-8, flow cytometry, colony formation assay, BODIPY staining, immunohistochemistry, western blotting, and qRT-PCR. ORMDL2 was upregulated in lower-grade glioma (LGG) and glioblastoma multiforme (GBM) tissues as compared to brain tissues collected from patients with traumatic craniotomy. Its high expression was associated with poor patient survival. ORMDL2 silencing inhibited glioma cell proliferation, lipid droplet formation, and promoted apoptosis in vitro. Silencing of ORMDL2 in xenograft mouse model inhibited tumor volume and weight. Additionally, ORMDL2 silencing decreased the levels of fatty acid metabolism-related factors FASN, ACC1 and SCD1, and the phosphorylation of a downstream effector of mTORC1 (S6K). Moreover, mTORC1 agonist MHY1485 reversed the inhibitory effect of ORMDL2 silencing on U87 cell fatty acid metabolism; mTORC1 inhibitor rapamycin reversed the promoting effect of ORMDL2 overexpression on U251 cell fatty acid metabolism. To conclude, this study demonstrated the high expression of ORMDL2 in glioma and its tumor-promoting action by activating mTORC1-mediated fatty acid metabolism.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Peat Moss (Sphagnum) in Reduction of Environmental Toxicity in Correlation with Various Microbes. 泥炭藓（Sphagnum）与各种微生物相关的环境毒性降低作用。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-09-01 DOI: 10.1007/s12010-025-05365-3

Pratik Talukdar, Snehika Sengupta, Aritra Nandi, Shaheen Sultana, Souvick Das, Swastika Mohanta, Milan Karmakar

{"title":"Role of Peat Moss (Sphagnum) in Reduction of Environmental Toxicity in Correlation with Various Microbes.","authors":"Pratik Talukdar, Snehika Sengupta, Aritra Nandi, Shaheen Sultana, Souvick Das, Swastika Mohanta, Milan Karmakar","doi":"10.1007/s12010-025-05365-3","DOIUrl":"https://doi.org/10.1007/s12010-025-05365-3","url":null,"abstract":"Peat moss (Sphagnum) plays a crucial role in extenuating the environmental toxicity by swaying the microbial activity and acting as a natural filter for removing pollutants. The peatlands help in the purification of water by filtering out the contaminants and decomposing organic matter by creating anaerobic conditions that create impacts on microbial communities. Additionally, Sphagnum pays for carbon sequestration that makes a positive impact in the carbon sinks process for peatlands. However, decomposition rates of peat vary based on various factors such as humidity, microbial population, and climate changes. It makes renewable sources of biofuel as it converts biofuels through the process of pyrolysis that make an alternative to fossil fuels. The parameters of pyrolysis, such as rate of nitrogen flow, reaction time, and temperature, enhance biofuel productivity. Moreover, the peatland ecosystem controls carbon cycles that help in reducing greenhouse gases like methane and carbon dioxide. Peat is mainly composed of humic substances that improve the nutrient retention and soil quality, creating importance for agriculture. The interconnection between microbes and Sphagnum aids in the absorption of heavy metals and environmental detoxification. However, degradation of peatlands because of overharvesting and climate changes threatens these benefits. Hence, sustainable management methods are required to maintain the integrity of peatlands while using their ecological importance. This study highlights the importance of Sphagnum and its microbial connection in removing pollutants, biofuel production, and carbon sequestration.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-Culture of Cyanobacteria and Rhizobia, Increasing Biomass Under Nitrogen-Starvation Conditions. 氮饥饿条件下蓝藻与根瘤菌共培养提高生物量。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-09-01 DOI: 10.1007/s12010-025-05353-7

Akari Takagi, Nanako Machida, Misato Nagao, Yu Kanesaki, Munehiko Asayama

{"title":"Co-Culture of Cyanobacteria and Rhizobia, Increasing Biomass Under Nitrogen-Starvation Conditions.","authors":"Akari Takagi, Nanako Machida, Misato Nagao, Yu Kanesaki, Munehiko Asayama","doi":"10.1007/s12010-025-05353-7","DOIUrl":"https://doi.org/10.1007/s12010-025-05353-7","url":null,"abstract":"A pair of new strains was obtained from a natural environment and identified as the filamentous non-heterocystous cyanobacterium SZ2 and the rhizobia Ensifer/Sinorhizobium sp. ST1. This pair was of particular interest because it could potentially perform both photosynthesis and nitrogen fixation. Interactions and material production were investigated in a new co-culture system using the \"natural pair\" of these strains. Cocultivation was favorable under both mixotrophic (coMC) and autotrophic conditions (coAC) when material production was assessed using heptadecane (C17H36) from the SZ2 strains. Under coAC, where nitrogen is depleted, some soluble factor(s) produced by the co-culture-possibly including ammonium ions-appear to function as biomass-increasing factors (BIFs), contributing to the enhanced accumulation of chlorophyll a in SZ2, along with extracellular polysaccharides, lipids, carbohydrates, and proteins in the total biomass. A notable feature of this \"aquatic\" coAC system is that it overcomes biomass reduction under nitrogen-deficient conditions, which is difficult in monoculture systems and can contribute to economic material production for biorefineries.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Nanocages as Building Blocks for Conjugated Supramolecular Materials Displaying Multitasking Properties. 蛋白质纳米笼作为显示多任务特性的共轭超分子材料的构建块。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-08-30 DOI: 10.1007/s12010-025-05364-4

Hugo César Santillán-Uribe, Iris Ashanty Soto-Valerio, Juan Carlos León-Contreras, Ismael Bustos-Jaimes

{"title":"Protein Nanocages as Building Blocks for Conjugated Supramolecular Materials Displaying Multitasking Properties.","authors":"Hugo César Santillán-Uribe, Iris Ashanty Soto-Valerio, Juan Carlos León-Contreras, Ismael Bustos-Jaimes","doi":"10.1007/s12010-025-05364-4","DOIUrl":"https://doi.org/10.1007/s12010-025-05364-4","url":null,"abstract":"Protein nanocages are a group of compartments naturally enclosing nucleic acids or proteins for biological purposes. Such materials have also inspired the design of novel proteins displaying self-assembling properties. The most studied protein nanocages are viral capsids and their derivative virus-like particles (VLPs), which consist of any or all of the structural proteins of the virion but lack nucleic acids and are therefore non-infectious. VLPs can be used as vaccine antigens or decorated with heterologous antigens to develop new vaccine materials. External surfaces of VLPs can also be decorated with chemical substances to impart new properties, like fluorescence tags or binding to cellular receptors. In addition, the internal space of VLPs can be used to encapsulate therapeutic materials that can be carried to specific cells or tissues. Although VLPs are naturally polyvalent and can display more than one decorating element, it is possible to expand the repertoire of decorating species by specifically conjugating different VLPs, which can be decorated with different functional elements. Here, VLPs of parvovirus B19 displaying different functional proteins were linked through the SpyTag-SpyCatcher biorthogonal conjugation technology. Characterizing the resulting species demonstrated the bioconjugation and the presence of the individual properties of each component. This proof-of-concept research implies that novel multitasking biomaterials can be constructed from protein nanocages and that the application landscape is vast and highly expandable.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Molecular Mechanisms of cSLE Through Integrated Serum Metabolomics, Network Pharmacology, and Molecular Docking Approaches. 通过整合血清代谢组学、网络药理学和分子对接方法揭示cSLE的分子机制。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-08-30 DOI: 10.1007/s12010-025-05376-0

Jinling Tang, Susu Wang, Zhu Wei

引用次数: 0

Evaluation of Benzothiazole-Chalcone Hybrids: Apoptosis Induction, Docking Analysis, and Anticancer Potential in Gastric Cancer Cells. 苯并噻唑-查尔酮复合物的评价：胃癌细胞凋亡诱导、对接分析和抗癌潜力。

IF 3.3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-08-23 DOI: 10.1007/s12010-025-05360-8

Ilker Kiliccioglu, Gorkem Dulger, Ahmad Badreddin Musatat, Alparslan Atahan, Emel Caliskan, Merve Alpay, Mustafa Zengin, Basaran Dulger

{"title":"Evaluation of Benzothiazole-Chalcone Hybrids: Apoptosis Induction, Docking Analysis, and Anticancer Potential in Gastric Cancer Cells.","authors":"Ilker Kiliccioglu, Gorkem Dulger, Ahmad Badreddin Musatat, Alparslan Atahan, Emel Caliskan, Merve Alpay, Mustafa Zengin, Basaran Dulger","doi":"10.1007/s12010-025-05360-8","DOIUrl":"https://doi.org/10.1007/s12010-025-05360-8","url":null,"abstract":"This study investigated a series of chalcone derivatives containing benzothiazole groups (C1-7) for their antimicrobial, antioxidant, and anticancer potential against gastrointestinal cancer cell lines. The compounds showed the highest antiproliferative effect in AGS gastric cancer cells compared to HCT116 colon cancer and HepG2 hepatocellular carcinoma cells. Among the tested compounds, C3 and C4 exhibited the most potent antiproliferative effects (IC50 = 7.55 µg/mL and 8.25 µg/mL at 48 h, respectively), significantly outperforming Cisplatin (IC50 = 15.71 µg/mL). Mechanistic investigations revealed that C3 and C4 induce apoptosis by upregulating caspase-3, -8, and -9, suppressing anti-apoptotic Bcl-2, and elevating pro-apoptotic Bax and p53 proteins. These compounds also impeded AGS cell migration. Antimicrobial evaluations demonstrated an effective profile against multi-drug resistant bacteria, and their effects were comparable to those of the reference antibiotic Ciprofloxacin (< 0.5 µg/mL). Antifungal activity results showed that MIC values ranged from < 0.5 to 256 mg/mL. Antioxidant profiling identified C1 as the most potent antioxidant, while C3 exhibited a unique dual role as an oxidant and pro-apoptotic agent. DFT computational studies harmonized the experimental findings, with molecular docking revealing high binding affinities of C3 and C4 to apoptosis regulators Bcl-2 and survivin. ADME predictions affirmed favorable drug-likeness, with moderate solubility, optimal distribution, and synthetic feasibility. This study provides a robust framework for developing benzothiazole-chalcone hybrids as precision therapeutics, positioning C3 and C4 as promising candidates for gastric cancer therapy.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0