Applied Biochemistry and Biotechnology最新文献_第2页

The Role of Hsa_circ_0087862/miR-149-5p/TRAF6 Regulatory Axis in Colorectal Cancer Progression. Hsa_circ_0087862/miR-149-5p/TRAF6调控轴在结直肠癌进展中的作用

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-14 DOI: 10.1007/s12010-025-05283-4

Qiu Xu, Yi Xu, Tianyao Yang, Yan Tang, Qiong Yang

{"title":"The Role of Hsa_circ_0087862/miR-149-5p/TRAF6 Regulatory Axis in Colorectal Cancer Progression.","authors":"Qiu Xu, Yi Xu, Tianyao Yang, Yan Tang, Qiong Yang","doi":"10.1007/s12010-025-05283-4","DOIUrl":"https://doi.org/10.1007/s12010-025-05283-4","url":null,"abstract":"Circular RNAs (circRNAs) have been reported to be associated with the progression of various tumors including colorectal cancer (CRC). However, the role and underlying mechanism of hsa_circ_0087862 in CRC remains unclear. Hsa_circ_0087862 expression in CRC tissues was analyzed using two GEO datasets (GSE138589 and GSE126094). Expression of hsa_circ_0087862, miR-149-5p and tumor necrosis factor receptor-associated factor 6 (TRAF6) in CRC cells was detected. The subcellular distribution of hsa_circ_0087862 was analyzed using a Cytoplasmic & Nuclear RNA Purification Kit. The function of hsa_circ_0087862 in CRC cells was detected using CCK-8, Transwell invasion assay, flow cytometry analysis, and Caspase-3 activity assay. The relationships between hsa_circ_0087862, miR-149-5p and TRAF6 were detected using luciferase reporter assay, RIP, or biotinylated RNA pull-down assay. Hsa_circ_0087862 was upregulated in CRC tissues and cells. Hsa_circ_0087862 is resistant to RNase R digestion and predominantly localized in the cytoplasm. Interference with hsa_circ_0087862 inhibited the malignant phenotypes of CRC cells by reducing cell proliferation and invasive abilities and triggering apoptosis. Hsa_circ_0087862 silencing inhibited TRAF6 expression by sponging miR-149-5p in CRC cells. Inhibition of miR-149-5p attenuated the effects of hsa_circ_0087862 on the malignant phenotypes of CRC cells. TRAF6 overexpression abolished the effects of miR-149-5p on cell growth, invasion and apoptosis in CRC cells. In conclusion, hsa_circ_0087862 silencing inhibited the malignant behaviors of CRC cells through inhibiting TRAF6 expression by sponging miR-149-5p.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-Assembled Immobilization and Metal-Polyphenol Network Encapsulation of β-Galactosidase on T4 Phage for Enhanced Biocatalytic Performance. β-半乳糖苷酶在T4噬菌体上的自组装固定和金属-多酚网络包封提高生物催化性能。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-14 DOI: 10.1007/s12010-025-05267-4

Dan Wu, Sulaiman Khan, Shujie Zhang, Huan Wang, Wei Chen, Shenqi Wang

{"title":"Self-Assembled Immobilization and Metal-Polyphenol Network Encapsulation of β-Galactosidase on T4 Phage for Enhanced Biocatalytic Performance.","authors":"Dan Wu, Sulaiman Khan, Shujie Zhang, Huan Wang, Wei Chen, Shenqi Wang","doi":"10.1007/s12010-025-05267-4","DOIUrl":"https://doi.org/10.1007/s12010-025-05267-4","url":null,"abstract":"Enzymes, key catalysts in biochemical reactions, are prone to denaturation under harsh conditions, leading to reduced stability and higher costs. Enzyme immobilization, using carriers like magnetic nanoparticles, metal-organic frameworks, and viruses, is a common solution. T4 bacteriophage, a virulent E. coli phage containing 155 Hoc and 870 Soc proteins, offers a cost-effective and highly stable platform for enzyme immobilization. In this study, Soc-β-galactosidase (Soc-β-gal) was immobilized on the surface of T4 bacteriophage via affinity fixation and further encapsulated with a metal-polyphenol network (MPN) coating. Comparative analysis of the biochemical properties revealed that the immobilized enzyme, β-gal T4, retained over 85% activity after 6 h at 50 °C, while free Soc-β-gal retained only 40.63%. Moreover, β-gal T4@TA-Ti demonstrated superior stability, retaining 92.88% of its activity after 6 h of UV exposure, compared to 10.21% for β-gal T4 and 7.23% for Soc-β-gal. The MPN coating also enhanced resistance to proteolytic degradation, with β-gal T4@TA-Ti retaining 9.48% of its activity after exposure to proteinase K, in contrast to 4.62% for β-gal T4. Overall, these results demonstrate that enzyme immobilization significantly enhances stability, while the MPN coating further improves resistance to extreme pH, ultraviolet radiation, and other environmental stressors, highlighting the potential of this approach for biocatalytic applications.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Anti-Biofilm Potential of Rhamnolipid Biosurfactant, Chemical Agents, and Coliphage Against E. coli Biofilm. 鼠李糖脂生物表面活性剂、化学制剂和噬菌体对大肠杆菌生物膜的抗生物膜电位比较。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-14 DOI: 10.1007/s12010-025-05257-6

Ajinkya M Thakare, Anuradha S Nerurkar

{"title":"Comparison of Anti-Biofilm Potential of Rhamnolipid Biosurfactant, Chemical Agents, and Coliphage Against E. coli Biofilm.","authors":"Ajinkya M Thakare, Anuradha S Nerurkar","doi":"10.1007/s12010-025-05257-6","DOIUrl":"https://doi.org/10.1007/s12010-025-05257-6","url":null,"abstract":"Biosurfactants are amphipathic microbial products that are released extracellularly or remain attached to the cell surface. The strong biofilm anti-adhesive and anti-biofilm properties of biosurfactants make them suitable candidates for application aimed at destroying troublesome bacterial biofilm. To investigate the anti-adhesion and biofilm disruptive properties of natural rhamnolipid biosurfactant, targeted isolation of a hydrocarbonoclastic bacteria from hydrocarbon-contaminated soil of Dakor, Gujarat, India, led to the isolation of bacteria producing biosurfactant, identified as Pseudomonas aeruginosa. DKR. The orcinol test preliminarily indicated that the biosurfactant was indeed rhamnolipid. The Fourier transform infrared spectroscopy and nuclear magnetic resonance further confirmed the biosurfactant as rhamnolipid. Pseudomonas aeruginosa DKR produced 25 mg/ml rhamnolipid that reduced the surface tension to 22.4 mN/m and possessed CMC (critical micellar concentration) of 130 mg/L. Sub-inhibitory dilution (0.25 mg/ml) of purified rhamnolipid DKR demonstrated superior antiadhesive and antibiofilm properties against biofilm-forming E. coli strains isolated from drinking water coolers in comparison to subinhibitory concentrations of common chemical surfactants, chelating agents, and weak acids used. Coliphage AM isolated on selected E. coli strains as hosts also demonstrated an appreciable biofilm anti-adhesive and anti-biofilm effect at 106 Pfu/ml. This study emphasizes the utility of rhamnolipid biosurfactant DKR and Coliphage AM in lieu of chemicals as natural and eco-friendly agents in applications to eradicate biofilm from drinking water cooling containers, etc.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Novel Laccase from Ganoderma lucidum and Application in Biotransformation to Bio-based Fragrances Using Alkaline Lignin as Raw Material. 新型灵芝漆酶的鉴定及其在以碱性木质素为原料生物转化为生物基香料中的应用。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-13 DOI: 10.1007/s12010-025-05251-y

Zhiping Zhang, Yue Zhang, Boli Xu, Tianxiao Li, Rongya Zhang, Tao Wei, Wu Wen

{"title":"Identification of Novel Laccase from Ganoderma lucidum and Application in Biotransformation to Bio-based Fragrances Using Alkaline Lignin as Raw Material.","authors":"Zhiping Zhang, Yue Zhang, Boli Xu, Tianxiao Li, Rongya Zhang, Tao Wei, Wu Wen","doi":"10.1007/s12010-025-05251-y","DOIUrl":"https://doi.org/10.1007/s12010-025-05251-y","url":null,"abstract":"A novel laccase, Lac3, was purified from Ganoderma lucidum fermentation broth by salting out, gel filtration chromatography, and Native-PAGE protein recovery. The molecular mass of Lac3 was 58.4 kDa as estimated by SDS-PAGE and exhibited catalytic properties with 2,2'-Biazobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) as substrate. The specific enzyme activity of Lac3 was determined to be 313.69 U/mg. The laccase was stable at temperatures < 65 °C and at pH of 2.5-4.5. The pH, temperature optima, Km and Vmax of the enzyme for ABTS oxidation were 3.0, 55 °C, 0.077 mM, and 2.98 mM/min, respectively. The metal ions and anions showed inhibitory effects on Lac3 activity except Cu2+ (1 mM). GC-MS analysis showed that various aroma products were generated by Lac3 treatment of alkaline lignin. The Lac3 and lignin model compounds had negative binding energy and hydrogen bonding. The analysis of docking suggested that Asp207, Asn256, and His459 play a key role in substrate binding and catalysis.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Approach for Improving Lutein Production of Chlorella sp. via Adaptive Evolution and Casein Acid Hydrolysate. 通过适应进化和酪蛋白酸水解提高小球藻叶黄素产量的综合方法。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-13 DOI: 10.1007/s12010-025-05279-0

Manlin Yan, Ting Liu, Quanyu Zhao

{"title":"Integrated Approach for Improving Lutein Production of Chlorella sp. via Adaptive Evolution and Casein Acid Hydrolysate.","authors":"Manlin Yan, Ting Liu, Quanyu Zhao","doi":"10.1007/s12010-025-05279-0","DOIUrl":"https://doi.org/10.1007/s12010-025-05279-0","url":null,"abstract":"Lutein is a high value-added product in microalgae. There are several strategies to improve its production, including isolation and selection of high-lutein producing algal strains; strain improvement by metabolic engineering, synthetic biology, or adaptive evolution; and metabolic regulation. In this study, an integrated approach was developed to improve lutein production by adaptive evolution and metabolic regulation. Firstly, a starting strain with a lutein content of ~5.18 mg/g was selected. Secondly, adaptive evolution was performed using an environmental stress of 300 mg/L phenol. The evolved strain, P30, was obtained after 30 cycles; its lutein content had increased to ~5.91 mg/g. Thirdly, the effects of 5-30 g/L NaCl on the lutein contents of the evolved strain were determined. We found that 10 g/L NaCl increased the lutein content by 11% and slightly inhibited growth. No significant changes were observed after adding 20-80 mg/L gibberellin under 10 g/L NaCl conditions. The addition of 10.5 mM casein acid hydrolysate (CAH) promoted the growth of the P30 strain under 10 g/L NaCl conditions. The lutein concentration of Chlorella sp. P30 was 6.91 mg/L at day 5, which was twice the lutein concentration of the starting strain at the same time. When osmotic pressure was removed, the lutein concentration was 8.42 mg/L. The results indicate that CAH supplementation enhances both microalgal growth and lutein biosynthesis. The results of this study provide a valuable reference for the metabolic regulation of lutein biosynthesis.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-10 DOI: 10.1007/s12010-025-05262-9

Bixiong Zhang, Lingli Xu, Yuran Ji, Jiezhuang Li, Liehui Liu, Liangfang Li, Zewei Zhuo, Zhongwen Zheng

{"title":"RHOGTPase-Related Gene Signature Predicts Prognosis, Immunotherapy Response, and Chemotherapy Sensitivity in Colon Cancer.","authors":"Bixiong Zhang, Lingli Xu, Yuran Ji, Jiezhuang Li, Liehui Liu, Liangfang Li, Zewei Zhuo, Zhongwen Zheng","doi":"10.1007/s12010-025-05262-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05262-9","url":null,"abstract":"Rho GTPases are known to promote colon cancer cell invasion and metastasis by modulating cell motility and adhesion. However, the clinical implications of Rho GTPase-related genes in prognosis and treatment response for colon cancer remain underexplored. We identified Rho GTPase-related prognostic genes using univariate Cox regression and applied Least Absolute Shrinkage and Selection Operator (LASSO) regression to refine these genes and develop a prognostic model. The Rho GTPase-related gene signature was analyzed for associations with clinical outcomes, immune status, immunotherapy, and chemotherapy response in colon cancer patients. A Rho GTPase-related 12-gene signature was established to predict prognosis across training and validation cohorts. Both univariate and multivariate analyses confirmed the Rho GTPase risk score as an independent prognostic factor. The model's area under the curve (AUC) and decision curve analysis (DCA) outperformed traditional TNM staging and several existing models. Immune cell analysis showed high Rho GTPase risk scores correlated with increased macrophage/monocyte and cancer-associated fibroblast infiltration. Tumor Immune Dysfunction and Exclusion (TIDE) analysis revealed an association between high Rho GTPase risk scores and immune dysfunction, suggesting potential resistance to immune checkpoint inhibitors in high-risk patients. Additionally, differential sensitivity to 59 chemotherapeutics was observed: high-risk patients showed greater sensitivity to CCT007093, CGP.082996, and AS601245, while low-risk patients were more sensitive to BMS.708163, NSC.87877, and Cisplatin, informing potential treatment choices. The Rho GTPase-related 12-gene signature offers a valuable tool for predicting prognosis and therapeutic response in colon cancer, supporting personalized treatment strategies and improved patient outcomes.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and Catalytic Characterization of β-1,3-Glucan Synthases of Mushroom Ganoderma lucidum. 蘑菇灵芝β-1,3-葡聚糖合成酶的结构与催化表征

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-09 DOI: 10.1007/s12010-025-05252-x

Zi-Ying Zhang, Lei Sun, JianYong Lei, Yu-Meng Yang, Wen-Jing Sun, Xin-Yi Zan, Li-Juan Meng, Feng-Jie Cui

{"title":"Structural and Catalytic Characterization of β-1,3-Glucan Synthases of Mushroom Ganoderma lucidum.","authors":"Zi-Ying Zhang, Lei Sun, JianYong Lei, Yu-Meng Yang, Wen-Jing Sun, Xin-Yi Zan, Li-Juan Meng, Feng-Jie Cui","doi":"10.1007/s12010-025-05252-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05252-x","url":null,"abstract":"β-Glucans are regarded as the key contributors to nutritional and supplementary credits of well-known edible/medicinal mushroom Ganoderma lucidum. However, the biosynthesis pathways of β-glucans and the involved key enzymes/genes in G. lucidum are still not well investigated. The present study cloned and bioinformatically analyzed a 5259-bp gene GLGLS1 and a 5334-bp gene GLGLS2 encoding membrane bound β-1,3-glucan synthases GLGLS1p and GLGLS2p with the predicted Mws of 199.64 kDa and 203.82 kDa from G. lucidum mycelia, respectively. GLGLS2p was further partially purified and identified from cultivated mycelia of G. lucidum as the potential key β-1, 3-glucan synthase for the β-glucan biosynthesis. GLGLS2p showed high specificity to UDP-glucose with a Km value of 79.54 µM and synthesize β-1, 3-glucan with a maximum degree of polymerization (DP) of 54. The AlphaFold 2 and molecular docking indicated that substrate UDP-glucose bound to seven amino acid residues of GLGLS2p via hydrogen bonds. These findings would provide insights into the knowledge of the glucan synthesis and catalytic mechanism of β-1,3-glucan synthases in G. lucidum.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Bacterial Laccase with Improved Catalytic Activity and Thermostability by Rational Design. 通过合理设计提高催化活性和热稳定性的工程细菌漆酶。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-09 DOI: 10.1007/s12010-025-05240-1

Xuting Sun, Xiaofan Lin, Yufan Xian, Faying Zhang, Lingxuan Zhu, Haitao Geng, Wenya Wang, Guimin Zhang

{"title":"Engineering Bacterial Laccase with Improved Catalytic Activity and Thermostability by Rational Design.","authors":"Xuting Sun, Xiaofan Lin, Yufan Xian, Faying Zhang, Lingxuan Zhu, Haitao Geng, Wenya Wang, Guimin Zhang","doi":"10.1007/s12010-025-05240-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05240-1","url":null,"abstract":"Laccases (benzenediol:oxygen oxidoreductases) are important multi-copper oxidases with widespread applications in industry. Here, Bacillus subtilis laccase CotA that has been widely studied was engineered to improve catalytic activity and thermostability via rational design. After iterative mutation of beneficial mutation sites, a triple mutant of CotA laccase (DTA) was obtained, whose catalytic activity and thermostability were improved by 2.7-fold and 1.4-fold compared with the wild-type (WT) CotA, respectively. The enhanced activity of DTA is primarily due to strengthened intermolecular forces in the active site, while its improved thermostability is attributed to increased hydrophobic residues, augmented protein surface flexibility, collectively rendering DTA a more active and stable enzyme with potential industrial applications. Compared to WT, DTA can degrade mycotoxins aflatoxin B1 (55.09% vs 44.51%) and ZEN (zearalenone) (71.59% vs 41.09%) more efficiently, and DTA can also better pretreat lignocellulose, promoting the hydrolysis of cellulose by cellulase. All these indicate that DTA has the potential to be used in industry.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and Risk Assessment of Polycyclic Aromatic Hydrocarbons in the Surface Water of Budhabalanga River Estuary, Chandipur, East Coast of India. 印度东海岸昌迪普尔布哈巴兰加河河口地表水中多环芳烃的赋存及风险评价

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-08 DOI: 10.1007/s12010-025-05248-7

Subhashree Nayak, Smruti Prajna Pradhan, Pratyusha Nayak, Sthitaprajna Nath Sharma, Nishigandha Muduli, Susri Nayak, Lipika Patnaik

引用次数: 0

Effect of Sarpogrelate on Cerebral Venous Sinus Thrombosis Model. 沙棘酸酯对脑静脉窦血栓形成模型的影响。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-07 DOI: 10.1007/s12010-025-05234-z

Xichen Wan, Ye Xiong, Shi Zhou Xing, Xiao Wu, Song Xiao, Peng Liu

{"title":"Effect of Sarpogrelate on Cerebral Venous Sinus Thrombosis Model.","authors":"Xichen Wan, Ye Xiong, Shi Zhou Xing, Xiao Wu, Song Xiao, Peng Liu","doi":"10.1007/s12010-025-05234-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05234-z","url":null,"abstract":"To elucidate the effects of cerebral venous sinus thrombosis (CVST) on mice and the effects of sarpogrelate (S) on CVST, mice were randomly divided into the sham-operated group, CVST group, and CVST + S group (sarpogrelate). Neurological function was evaluated using the rotarod test, balance beam test, and open-field test. Moreover, laser speckle contrast imaging was employed to observe the blood flow in the cerebral cortex of mice, and immunofluorescence was used to quantify the neurons. In the neurofunctional assessment tests (i.e., rotarod test, balance beam test, and open-field test), the CVST group exhibited poorer performance compared to the sham-operated group. However, mice treated with sarpogrelate showed significantly better performance than the CVST group (P < 0.05). Moreover, a significant decrease in cerebral blood flow was observed in the CVST group compared to the sham-operated group (P < 0.01). In contrast, a significant increase in blood flow was observed in the CVST + S group compared to the CVST group (P < 0.05). Furthermore, the number of neurons in the hippocampus and prefrontal cortex of mice in the CVST group was lower than that in the sham group (P < 0.05), and the number of neurons in the hippocampus and prefrontal cortex of the CVST + S group was higher than that of the CVST group (P < 0.05). Sarpogrelate can increase blood flow in the cerebral cortex of mice with CVST, leading to decreased neuronal cell damage and improved neurological function.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0