Applied Biochemistry and Biotechnology最新文献

{"title":"Characterization of a New Glycolipopeptide Biosurfactant Produced by a Chrysene-Degrading Strain Achromobacter aegrifaciens.","authors":"Assia Lazzem, Haykel Galai, Ahmed Landoulsi, Abdelwaheb Chatti, Alya El May","doi":"10.1007/s12010-025-05247-8","DOIUrl":"https://doi.org/10.1007/s12010-025-05247-8","url":null,"abstract":"<p><p>Biosurfactants are surface-active biomolecules with emulsifying properties. These versatile compounds have numerous applications across various industries and environmental contexts, including the remediation of areas contaminated by persistent pollutants such as hydrocarbons. This study focuses on the physicochemical characterization of a biosurfactant produced by a newly identified chrysene-degrading Achromobacter aegrifaciens strain. Biosurfactant production was evaluated using the emulsification index (E24). The research determined the biosurfactant's surface tension, critical micelle concentration (CMC), and stability under different temperatures, pH levels, and salt concentrations. The chemical components of the partially purified biosurfactant were analyzed using biochemical tests, thin layer chromatography (TLC), Fourier transform infrared (FTIR), nuclear magnetic resonance (H¹NMR) spectroscopy, and gas chromatography-mass spectrometry (GC-MS). After 96 h of incubation, A. aegrifaciens S5 yielded 1.68 g/L of biosurfactant, with a CMC of 50 mg/L. The isolated compound was identified as a novel glycolipopeptide biosurfactant, demonstrating high efficiency with an E24 of 88.4% and the ability to reduce the surface tension of water from 70.3 to 38 mN/m. The biosurfactant exhibited functional stability across temperatures from 4-80 °C, pH levels from 2 to 12, and salt concentrations of 1-10% while maintaining structural integrity up to 120 °C. Given these characteristics, the biosurfactant shows a promising potential for applications in petroleum, detergent, and food industries.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"rhGALNS Enzyme Stability in Physiological Buffers: Implications for Sustained Release.","authors":"Samuel Ruesing, Samuel Stealey, Qi Gan, Linda Winter, Adriana M Montaño, Silviya P Zustiak","doi":"10.1007/s12010-025-05266-5","DOIUrl":"https://doi.org/10.1007/s12010-025-05266-5","url":null,"abstract":"<p><p>Morquio A syndrome is a rare genetic disorder where deficiency in N-acetylgalactosamine-6-sulfate sulfatase (GALNS) enzyme prevents breakdown of glycosaminoglycans (GAGs). Recombinant human GALNS (rhGALNS) is currently administered by intravenous infusion, but the treatment is costly and time-consuming and provides limited efficacy. Patient quality of life could be improved by an injectable sustained rhGALNS release device that would eliminate weekly multi-hour infusions. Polyethylene glycol (PEG) hydrogels can be employed as a hydrophilic, tunable, non-toxic, and biodegradable drug delivery system for the sustained release of rhGALNS, as explored by us previously. Here, we investigated the stability of rhGALNS in various buffers mimicking the in vivo environment that would be encountered by the enzyme, inside of and outside the PEG hydrogels. rhGALNS activity was reduced 85% by reversible inhibition in phosphate-buffered saline (PBS), representing interstitial fluid and plasma. Buffer exchanging into acidic buffer representing the lysosome recovered this loss. However, incubation in PBS for 3 days resulted in an irreversible loss of 85%. There were no significant changes in rhGALNS hydrodynamic radius upon activity loss, suggesting structural integrity. Such activity loss makes sustained delivery impractical without additional stabilization, such as confinement within the hydrogel. rhGALNS activity was retained upon encapsulation, and the average specific activity of rhGALNS released from a hydrogel decreased only 20% over 7 days. These results show that the activity of rhGALNS was better retained within the hydrogel than in buffer alone, potentially enabling sustained release for rhGALNS or other enzymes unstable in physiological conditions with our hydrogel delivery device.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple and Cost-effective Fluoride Riboswitch-Based Whole-Cell Biosensor for the Determination of Fluoride in Drinking Water.","authors":"Madushani R Ariyarathna, Jayani P Nissanka, Kasun Methlal, Kasun D Abeyrathne, Madhusha Satharasinghe, P Banushan, Dilhara Manawadu, Gayan Mirihana Arachchilage, Gayathri N Silva","doi":"10.1007/s12010-025-05269-2","DOIUrl":"https://doi.org/10.1007/s12010-025-05269-2","url":null,"abstract":"<p><p>Fluoride in drinking water poses health risks when exceeding WHO-recommended limits, necessitating accurate detection methods. This study developed a highly selective fluoride riboswitch (FRS)-based whole-cell biosensor using an E. coli mutant with a recombinant plasmid carrying the FRS fused to the lacZ reporter gene. Fluoride binding to the FRS aptamer triggers β-galactosidase expression, enabling fluoride quantification by a colorimetric assay. Using a calibration curve (R² > 0.9), fluoride concentrations in Sri Lankan water samples were determined (0.15-0.90 ppm). Minimal interference from common ions (Cl⁻, OH⁻, Na⁺, K⁺, Ca²⁺, CO₃²⁻, HCO₃⁻, and NO₃⁻) confirmed the biosensor's narrow specificity and high selectivity towards fluoride.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Characterization of a Novel, Glucose-Tolerant β-Glucosidase from Jiangella ureilytica KC603, and Determination of Resveratrol Production Capacity from Polydatin.","authors":"Arife Kaçıran, Miray Şahinkaya, Dilşat Nigar Çolak, Numan Saleh Zada, Murat Kaçağan, Halil İbrahim Güler, Hayrettin Saygın, Ali Osman Beldüz","doi":"10.1007/s12010-025-05272-7","DOIUrl":"https://doi.org/10.1007/s12010-025-05272-7","url":null,"abstract":"<p><p>Β-glucosidase, a ubiquitous enzyme, is responsible for catalyzing the hydrolysis of β-glycosidic linkages present in polysaccharides and contributes significantly to several industrial sectors such as food, agriculture, and biofuel production. β-glucosidases can convert polydatin to resveratrol through de-glycosylation. Resveratrol is important for human health and has potential applications in pharmacology. The preference of enzymatic conversion methods for resveratrol production improves the importance of β-glucosidases. The glucose tolerance of β-glucosidases also significantly impacts their applicability. Because the inhibition of many β-glucosidase's activity by their reaction product, glucose, is a limiting factor for industrial applications. In this study, a robust β-glucosidase was isolated from a novel-defined Jiangella ureilytica KC603 strain. The β-glucosidase encoding gene (JurBglKC603) was cloned and expressed in E. coli BL21 (DE3) cells and a 50.1 kDa protein was purified using Ni-affinity column chromatography. The efficient polydatin deglycosylation capacity of JurBglKC603 was determined by Glucose Oxidase-Peroxidase (GOPOD) assay. JurBglKC603 exhibits remarkable resistance to glucose concentrations of up to 3 M. The enzyme remained active across a broad pH spectrum and was unaffected by most heavy metal ions, except for Hg²⁺. The kinetic parameters of JurBglKC603 were K_m = 0.44 mM, V_max = 26.87 U·mg^-1, k_cat = 21.1 s^-1, and k_cat/K_m = 47,954 M^-1·s^-1 against pNPG and K_m = 4.6 mM, V_max = 20 U·mg^-1, k_cat = 17.2 s^-1, and k_cat/K_m = 3822 M^-1·s^-1 against polydatin. Molecular docking studies have demonstrated that Gln19, His120, Trp411, and Glu410 play a vital role in the interaction with polydatin.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Production of Prodigiosin Utilizing Agro-industrial Yellow Mustard Cake Waste by a Novel Strain Serratia marcescens SBL03.","authors":"Nisarg Gohil, Deepak Siruka, Darshana Musini, Kinjal Makwana, Khushal Khambhati, Gargi Bhattacharjee, Ravi Kalariya, Ali Abusharha, Suresh Ramakrishna, Vijai Singh","doi":"10.1007/s12010-025-05259-4","DOIUrl":"https://doi.org/10.1007/s12010-025-05259-4","url":null,"abstract":"<p><p>Prodigiosin is a red-hued bacterial pigment with promising anticancer, antimalarial, antiparasitic, and antibacterial therapeutic properties. However, the commercial exploitation of this secondary metabolite is hindered due to high production cost and poor yield. In this study, the prodigiosin-producing Serratia marcescens SBL03 strain was isolated and identified. The pigment production capability was evaluated first in a complex commercial media, and thereafter in eight different agro-industrial waste materials as the sole source of nutrients. Among them, yellow mustard cake (YMC) medium titred 2.44 g/L prodigiosin, which was 7.62-fold higher than the higher pigment production achieved in the complex media, i.e., the nutrient broth (0.32 g/L of prodigiosin). The prodigiosin production was further enhanced through fermentation optimization exploring the Box-Behnken design (BBD) model and as high as 4.554 g/L titre of prodigiosin was achieved utilizing 1.06% w/v YMC at 28.3 °C temperature after 116 h and 42 min of incubation. This study is a small step towards a circular bioeconomy market with biovalorization of YMC medium for sustainable production of prodigiosin in a reasonable manner.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Sensitivity Blood Cell Detection via Terahertz Refractive Index Sensing in Biomedical Applications.","authors":"Md Anowar Kabir, Md Selim Hossain, Hasan Abdullah, Shuvo Sen","doi":"10.1007/s12010-025-05274-5","DOIUrl":"https://doi.org/10.1007/s12010-025-05274-5","url":null,"abstract":"<p><p>Biosensors are essential tools for detecting and analyzing various elements of human biology. This study introduces an innovative circular-shaped photonic crystal fiber (PCF) with a hexahedron core for the precise detection of blood components. The sensor's performance evaluated using COMSOL Multiphysics software. The finite element methods (FEM) is applied to solve Maxwell's equations and perform simulations across a terahertz (THz) frequency range from 1.0 to 3.0 THz. This comprehensive investigation focuses on optimizing several important optical parameters, including relative sensitivity (RS), confinement loss (CL), effective mode area (EMA), and birefringence, etc. for enhancing the detection of various blood components. The Optical sensor is constructed by  Topas as cladding material. The sensor demonstrates exceptional performance with RS of approximately 95.02% for glucose, 95.48% for plasma, 96.30% for white blood cells (WBCs), and 97.04% for red blood cells (RBCs) at an operational frequency of 2.20 THz. Thus the proposed sensor can provide reliable and accurate measurements across different blood components in advanced biomedical applications.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Tetracycline Degradation by Laccase Immobilized on Carboxymethyl Starch Magnetic Nanoparticles: Optimization of Degradation Conditions, Reusability, and Degradation Pathways.","authors":"Suhaily Suhaimi, Nardiah Rizwana Jaafar, Nashriq Jailani, Norzita Ngadi, Roshanida A Rahman, Rosli Md Illias","doi":"10.1007/s12010-025-05238-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05238-9","url":null,"abstract":"<p><p>Bioremediation using laccase (Lac) to degrade tetracycline (TC) contaminant is promising due to high specificity and selectivity of the biocatalyst. However, degradation parameters should be carefully studied to achieve maximum degradation efficiency. Thus, the current study aimed to degrade TC by employing Lac immobilized on novel carboxymethyl starch magnetic nanoparticles (CMS-MNP). The maximum TC degradation was determined via one-factor-at-a-time (OFAT) and central composite design (CCD). Using OFAT, the maximum TC degradation (56.3%) was achieved at initial TC concentration of 20 mg/mL, pH 6, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) concentration of 1.2 mM, and 10 h of reaction time. When CCD was employed, the TC degradation increased by one-fold with the highest TC degradation (62.1%) recorded at initial TC concentration of 25 mg/L, pH 5.7, ABTS concentration of 1.0 mM, and 11.8 h of reaction time. The CMS-MNP-Lac was reused for 7 cycles with a total TC degradation of 73 mg/L. The TC degradation in this study demonstrated outstanding potential and provided a green alternative for the treatment of TC contaminants in the environment.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning Empowering Microbial Cell Factory: A Comprehensive Review.","authors":"Dechun Kong, Jinyi Qian, Cong Gao, Yuetong Wang, Tianqiong Shi, Chao Ye","doi":"10.1007/s12010-025-05260-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05260-x","url":null,"abstract":"<p><p>The wide application of machine learning has provided more possibilities for biological manufacturing, and the combination of machine learning and synthetic biology technology has ignited even more brilliant sparks, which has created an unpredictable value for the upgrading of microbial cell factories. The review delves into the synergies between machine learning and synthetic biology to create research worth investigating in biotechnology. We explore relevant databases, toolboxes, and machine learning-derived models. Furthermore, we examine specific applications of this combined approach in chemical production, human health, and environmental remediation. By elucidating these successful integrations, this review aims to provide valuable guidance for future research at the intersection of biomanufacturing and artificial intelligence.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Phospholipase D-Producing Bacillus cereus: Taxonomy, Mutagenesis, Fermentation Optimization and Enzyme Characterization.","authors":"Ying He, Ao Huang, Yun Liu","doi":"10.1007/s12010-025-05278-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05278-1","url":null,"abstract":"<p><p>Phospholipase D (PLD) is a valuable enzyme in industrial processes for converting phosphatidylcholine (PC) to phosphatidylserine (PS). In this study, a strain of Bacillus cereus was isolated from soil and identified through 16S rRNA sequencing. To enhance PLD activity, various mutagenesis strategies-including chemical treatment, irradiation, and their combinations-were employed, resulting in four high-activity positive mutants (C-7, I-12, CI 7-12, and IC 13-14). Among these, the CI 7-12 mutant exhibited a significantly higher enzymatic activity, showing a 3.12-fold increase (312.2%) compared to the wild-type strain. Fermentation conditions were optimied using response surface methodology (RSM), achieving a PLD activity of 35 U/mL. The enzyme demonstrated stability over a wide temperature range (30-60 °C) and pH range (6-10), with a half-life of 128 days. Kinetic analysis revealed a V_max of 20.04 μmol/h and a K_m of 7.13 μmol/mL, indicating efficient activity. In bioconversion experiments, the PLD-enriched fermentation broth catalyzed the conversion of PC to PS, achieving a 53.0% conversion rate and a 92.3% selectivity for PS in a two-phase system. These findings expand the potential sources of PLD and underscore its applicability for PS production in biotechnological applications.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation on the Detoxification of Indoxyl Sulfate (IS) and Indole-3-Acetic Acid (IAA) Protein-Bound Uremic Toxins (PBUTs) Using Trametes versicolor Biocompatible Laccase: In Situ Synchrotron Imaging, Experimental and Computational Studies.","authors":"Sana Daneshamouz, Shaghayegh Saadati, Sishi Zhu, Denis Kalugin, Ahmed Shoker, Amira Abdelrasoul","doi":"10.1007/s12010-025-05235-y","DOIUrl":"https://doi.org/10.1007/s12010-025-05235-y","url":null,"abstract":"<p><p>The removal of protein-bound uremic toxins (PBUTs), such as indoxyl sulfate (IS) and indole-3-acetic acid (IAA), from hemodialysis (HD) patients remains a significant challenge due to their strong binding to serum proteins, such as albumin. This study aimed to evaluate the potential of using the enzyme laccase, derived from Trametes versicolor, for the decomposition and removal of IS and IAA during HD. Molecular docking was utilized to investigate the interactions between laccase and the toxins, identifying key functional groups involved. To assess the detoxification efficacy, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) were employed, allowing for the identification of decomposition byproducts and their toxicity assessment. Additionally, in situ synchrotron radiation micro-computed tomography (SR-µCT) at the Canadian Light Source (CLS) was used to analyze the binding of human serum albumin (HSA) with IS and IAA before and after laccase treatment. Our findings revealed that laccase effectively decomposed IAA into five byproducts, including indole, as confirmed by GC-MS, while IS remained unaffected. The byproducts exhibited lower toxicity ratings than IAA and were more easily eliminated through HD. However, synchrotron-based μ-CT analysis showed reduced HSA-IAA adsorption on the HD membrane post-laccase treatment, with no impact on HSA-IS adsorption. Notably, the transformation of indole into IS in the liver suggests that laccase may not be suitable for IAA detoxification in HD. Despite the lack of expected outcomes, these results provide valuable insights into toxin-enzyme interactions and guide future research toward alternative strategies for PBUTs removal in HD.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}