Applied Biochemistry and Biotechnology最新文献

{"title":"Efficient Expression of Small Molecule Bioactive Peptides in Bacillus licheniformis.","authors":"Hanchao Zhang, Lanying Shao, Qinghua Feng, Qingping Guo, Haikuan Wang, Huitu Zhang","doi":"10.1007/s12010-026-05885-6","DOIUrl":"https://doi.org/10.1007/s12010-026-05885-6","url":null,"abstract":"<p><p>Stable expression of γ-Glutamyltranspeptidase (GGT)-BPC157 and mScarlet-BPC157 in Bacillus licheniformis strain 2709 by chromosomal integration. Fermentation conditions were optimized using single-factor and orthogonal experiments to maximize yield. Under optimal conditions (2% inoculum, 40 g/L soybean peptone, and 80 g/L glucose), reporter-based expression signals increased approximately threefold compared to the basal medium. The fusion proteins were purified by fractional ammonium sulfate precipitation; optimal saturations were 50% and 60%, respectively. Biological activity was assessed in a rat model of ethanol-induced acute gastric ulcer. Both proteins showed gastroprotective and therapeutic activity, with ulcer inhibition rates ranging from 75.3% to 82.9% protective regime and 80.4% to 84.6% therapeutic regime. Histological analysis showed that the treatment reduced mucosal inflammation and stimulated glandular repair. ELISA showed significant downregulation of proinflammatory cytokines TNF-α, IL-1β, and IL-6 in gastric tissue. These results suggest a biotechnological production strategy for BPC 157 and applications for gastric ulcer treatment.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148856802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential and Toxicological Impact of Graphene Quantum Dot Nanocomposites in Adult Zebra Fish.","authors":"K R Preethy, M Chamundeeswari, P Kowsalya, R Venkatesan","doi":"10.1007/s12010-026-05889-2","DOIUrl":"https://doi.org/10.1007/s12010-026-05889-2","url":null,"abstract":"<p><p>Fabricated graphene quantum dots (FQD) are popularly used in biomedical applications, as well as for biosensing, drug administration, and anticancer therapy. Despite the promising biomedical applications of graphene quantum dots, their long-term biocompatibility and toxicological safety remain insufficiently understood. Therefore, this study aimed to develop a bioenhanced FQD nanocomposite and evaluate its biocompatibility, wound healing potential, and toxicity using in vitro endothelial cells and adult zebrafish models. In silico molecular docking confirmed favourable binding to the wound healing target protein (-3.526 kcal/mol), supporting the therapeutic potential of FQDs. The toxicity and safety of employing these materials, however, must be further evaluated due to their unfavourable side effects. The primary goal of the current investigation was to examine in adult Zebrafish (Danio rerio) the toxicities and wound-healing capacity of FQD. Cytotoxicity and scratch wound migratory assay demonstrated significant result with the concentration of 5 µg/ ml. The live model was exposed to different sample concentrations ranging from 1 to 25 µg/mL for the evaluation period of 14 days to examine the samples chronic effects. The acute toxicity in vital organs was examined and the result demonstrates defined morphology of cardiomyocytes, normal distribution of hepatocytes, densely packed parenchymal architecture of pancreatic artery and uniformly distributed neuronal cells. Up to the concentration of 5 µg results were closer to control groups with nil mortality. The end of 48 h observation concentration dependent efficacy was observed closer to control groups for exhibiting excellent wound healing percentage.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148856842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citronellal Alleviates Cognitive Impairment by Inhibiting NHE1-Mediated Mitochondrial Damage in Vascular Dementia.","authors":"Qian-Qian Wang, Hong-Dou Zhang, Meng-Yao Zhao, Pei-Yao Liu, Chun-Rui Zhang, Hui-Jun Liu, Guang-Qiu Ren, Xue-Yu Sun, Xi-Yue Li, Xue Zhang, Rui Hao, Peng Li, Ya-Ling Yin, Jing Yang, Ling Wang","doi":"10.1007/s12010-026-05871-y","DOIUrl":"https://doi.org/10.1007/s12010-026-05871-y","url":null,"abstract":"<p><p>The present study aimed to explore the protective effect of citronellal (CT) on cognitive impairment and its underlying mechanism involving NHE1‑dependent mitochondrial damage both in vivo and in vitro. In vivo, a total of 36 rats (n = 6 per group) were randomly assigned to six groups: Sham, VaD, Sham + CT, Sham+LiCl, VaD + CT, and VaD + CT+LiCl. The VaD model was established by permanent bilateral common carotid artery occlusion. Learning and memory were evaluated using the Morris water maze test. Histopathological changes in the hippocampal CA3 region were examined by Hematoxylin and eosin, Neuronal Nuclei (NeuN), and Golgi-Cox staining. Apoptosis and proliferation were detected by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining and 5-Ethynyl-2'-deoxyuridine (EdU) staining, respectively. Mitochondrial ultrastructure and membrane potential were assessed by Transmission electron microscope and JC-1 staining. The reactive oxygen species (ROS), nitric oxide (NO), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured by dihydroethidium staining, water-soluble tetrazolium, thiobarbituric acid, and nitrate reductase methods, respectively. Protein expression was determined by immunohistochemistry, immunofluorescence, and Western blot. In vitro, Mouse hippocampal neuronal cells were treated with H₂O₂ or CT at 25-400 µM or 1-20 µg/L. Cell viability, mRNA levels, mitochondrial respiratory efficiency, membrane potential, and ROS levels were measured by reverse transcription quantitative polymerase chain reaction (RT-qPCR), JC-1, and Mito-SOX staining. Protein expression was detected by immunofluorescence and Western blot. In vivo findings demonstrated that CT alleviates cognitive impairment and regulated the expression of proteins within the NHE1/PI3K/AKT signaling axis (F (5, 12) = 203.3, P < 0.01). In vitro, H₂O₂ impaired mitochondrial function and dysregulated apoptosis- and NHE1-related signaling in HT-22 cells, while CT treatment significantly reversed these alterations (F (3, 20) = 93.02, P < 0.05). CT prevented mitochondrial dysfunction, mitochondrial apoptosis and oxidative injury both in vitro and in vivo by regulating the NHE1/PI3K/AKT signaling axis.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin-loaded PEGylated Magnetic Iron Oxide Nanoparticles: a Biogenic Platform for Targeted and Controlled Drug Release.","authors":"Krishnan Vijayalakshmi, Alagappan Kavitha, Srinivasan Ayyanaar","doi":"10.1007/s12010-026-05882-9","DOIUrl":"https://doi.org/10.1007/s12010-026-05882-9","url":null,"abstract":"<p><p>The development of environmentally sustainable and targeted nanocarriers is crucial for improving the therapeutic efficacy of anticancer agents while reducing systemic toxicity. Here we successfully synthesized curcumin (CUR) loaded polyethylene glycol (PEG) functionalized magnetic iron oxide nanoparticles (Fe₃O₄@PEG-CUR-NPs) by a green biogenic approach using Hibiscus rosa-sinensis flower extract and evaluated as a multifunctional platform for controlled drug delivery and cancer therapy. UV-Vis, FTIR, PXRD, SEM, TEM, DLS, TGA and VSM characterizations have been performed comprehensively to confirm the successful fabrication of crystalline, spherical nanoparticles with average size of 10-15 nm, excellent colloidal stability (zeta potential - 31.5 mV) and retained magnetic responsiveness with saturation magnetization of 28.30 emu/g. The nanocarrier showed significant pH-responsive drug release, with 90.55% cumulative CUR release under acidic conditions (pH 4.5) compared to 44.5% at physiological pH (7.4), indicating its possibility for tumor-targeted delivery. Release kinetic studies revealed that the drug release was mainly diffusion-controlled and followed a non-Fickian transport mechanism. Besides, Fe₃O₄@PEG-CUR-NPs showed good anti-inflammatory effect with IC₅₀ value of 25.10 μg/mL, which was significantly better than diclofenac (IC₅₀ = 82.20 μg/mL). In vitro cytotoxicity assays showed potent and dose dependent anticancer activity against A549, MDA-MB-231 and MCF-7 cell lines with IC₅₀ values of 50.2, 10.5 and 6.7 μg/mL respectively, indicating an increased susceptibility of breast cancer cells. The synergistic combination of green synthesis, magnetic targeting capability, pH-triggered drug release, and superior anticancer efficacy highlights Fe₃O₄@PEG-CUR-NPs as a promising nanotherapeutic platform for precision cancer treatment and advanced biomedical applications.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Multi-Omics Analysis of Gut Microbiota-Associated Metabolites and Related Host Molecular Signatures in Cervical Squamous Cell Carcinoma.","authors":"Bin Chen, Changchang Xu","doi":"10.1007/s12010-026-05891-8","DOIUrl":"https://doi.org/10.1007/s12010-026-05891-8","url":null,"abstract":"<p><p>Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) is a common female malignancy. Gut microbiota and metabolites are critical regulators of tumor immunity and therapy, but their roles in CESC remain unclear. This study integrated TCGA and GEO datasets with gut microbiota information to construct a protein-protein interaction network. These targets were prioritized using machine learning, SHAP interpretation, and Mendelian randomization analysis. Immune-related pathways were explored using single-cell and spatial transcriptomic analyses. Molecular docking and molecular dynamics simulations were performed to evaluate potential interactions between key metabolites and their targets. A total of 136 gut microbiota-related DEGs were identified, which may be associated with intercellular immune interactions. KDR was selected as a core target and may exert protective effects. Single-cell and spatial transcriptomic analyses suggested that specific metabolites may be associated with changes in the tumor microenvironment potentially involving the MIF signaling pathway. Network analysis of microbes, metabolites, and targets suggested that metabolites such as 5-(3,4-dihydroxyphenyl) pentanoic acid may serve as key mediators linking gut microbiota and KDR signaling. Additionally, eight non-toxic metabolites with favorable drug-likeness were identified, molecular docking and molecular dynamics simulation demonstrated stable binding to KDR with potential bioactivity, providing a theoretical basis for developing microbiota-related therapeutic strategies. The gut microbiota and its metabolites may be associated with the immune microenvironment and tumor progression in CESC, potentially involving the MIF signaling axis. This study provides a computational framework and preliminary evidence supporting microbiota-related hypotheses, and may inform future experimental investigations and therapeutic strategy development.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Biomedical Evaluation of ZnO/Ag/Ag₂O@chitosan Nanocomposite for Infection Control and Tissue Regeneration.","authors":"Khadija Hameed, Amina Asif, Aisha Rafique, Muhammad Shahid Nazir, Naveed Ahmad, Zulfiqar Ali, Fiaz Hussain, Sadaf Ul Hassan","doi":"10.1007/s12010-026-05886-5","DOIUrl":"https://doi.org/10.1007/s12010-026-05886-5","url":null,"abstract":"<p><p>In recent years, the multifunctional nanocomposites for biomedical applications have attracted significant interest. A biocompatible matrix of chitosan was selected as a medium for the synthesis of a ZnO/Ag/Ag₂O nanocomposite in this work, which was performed by a solid-state method.The successful synthesis and electronic structure of the ZnO/Ag/Ag₂O@chitosan nanocomposite was confirmed by FTIR, XRD, UV-Vis, and SEM analyses.The XRD data showed that the crystalline ZnO, Ag, and Ag₂O phases were successfully incorporated into the chitosan matrix with sharp peaks indicating the increased crystallinity of the incorporated phases compared to the pristine chitosan. The nanocomposite showed strong antibacterial activity against both Gram-positive and Gram-negative bacteria, which indicates synergistic effects between the two nanoparticles, ZnO and Ag/Ag₂O. Anti-inflammatory assessments showed significant reduction in inflammatory markers and in vivo wound healing experiments showed accelerated tissue regeneration and re-epithelialization. The effectiveness of nanocomposite was also confirmed through histopathological analysis, which demonstrated a decrease in inflammation and enhancement in tissue design in these treated wounds. The multifunctional properties of ZnO/Ag/Ag₂O@chitosan make it a promising material for advanced wound care and other biomedical applications.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical and Molecular Insights Into the Anti-PCOS Potential of Anchusa Strigosa: Targeting Hormones, Biochemical Markers, and WNT Genes.","authors":"Wajid Mohammad Sheikh, Jasmeena Jan, Atif Khurshid Wani, Majid Shafi, Ishraq Hussain, Indrani Jadhav, Showkeen Muzamil Bashir","doi":"10.1007/s12010-026-05887-4","DOIUrl":"https://doi.org/10.1007/s12010-026-05887-4","url":null,"abstract":"<p><p>Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder characterized by reproductive dysfunction, oxidative stress, and metabolic abnormalities. The present study investigated the therapeutic efficacy of a hydroalcoholic extract of Anchusa strigosa in a letrozole-induced PCOS rat model and explored its effects on ovarian Wnt signaling. Female Wistar rats (n = 36) were divided into normal control, PCOS control, A. strigosa low-dose (100 mg/kg), A. strigosa high-dose (250 mg/kg), and metformin (50 mg/kg) groups. Phytochemical profiling by FTIR and GC-MS identified 114 bioactive compounds enriched with phenolic, thiol, and isothiocyanate functional groups. Letrozole administration significantly increased body weight from 174.33 ± 1.80 g to 249.66 ± 1.66 g and elevated malondialdehyde levels from 45.42 ± 1.92 to 70.20 ± 1.51 while reducing superoxide dismutase from 28.97 ± 0.55 to 16.82 ± 0.59 and glutathione from 4.09 ± 0.07 to 1.83 ± 0.10 compared with normal controls. Treatment with A. strigosa, particularly at 250 mg/kg, markedly restored estrous cyclicity, reduced final body weight to 225.27 ± 0.57 g, decreased malondialdehyde to 60.90 ± 0.48, improved superoxide dismutase to 22.57 ± 0.27 and glutathione to 3.22 ± 0.09, normalized reproductive hormones and lipid profiles, restored ovarian morphology, and significantly upregulated ovarian Wnt7a and Wnt8b gene and protein expression. Although metformin produced slightly superior therapeutic effects, A. strigosa demonstrated comparable efficacy across most reproductive and metabolic parameters, highlighting its multi-target pharmacological potential. These findings suggest that A. strigosa represents a promising phytotherapeutic candidate for PCOS management. Future studies should focus on isolation of its active constituents, elucidation of the molecular mechanisms underlying Wnt pathway modulation, long-term safety evaluation, and well-designed clinical trials to establish its translational applicability in human PCOS.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148811857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesized zinc oxide nanoparticles from Sargassum muticum: characterization and biological assessment of anti-dermatophytic, antioxidant, and cytotoxic activities.","authors":"Bouchra Benhniya, Fatima Zahra Karmil, Fatima Lakhdar, Badr-Ddine El Mouns, Noreddine Rezzoum, Samira Etahiri","doi":"10.1007/s12010-026-05884-7","DOIUrl":"https://doi.org/10.1007/s12010-026-05884-7","url":null,"abstract":"<p><p>The valorization of invasive marine macroalgae represents a sustainable strategy for developing eco-friendly bioactive materials. In this study, zinc oxide nanoparticles (ZnSm-NPs) were green-synthesized using an aqueous extract (AE-Sm) of the invasive brown seaweed Sargassum muticum collected from the Moroccan Atlantic coast. The synthesized nanoparticles were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDS), high-resolution transmission electron microscopy (HR-TEM), and selected area electron diffraction (SAED). The characterization analyses confirmed the successful formation of crystalline ZnO nanoparticles with a hexagonal wurtzite structure and predominantly spherical to rod-shaped morphology. The biological activities of ZnSm-NPs were then evaluated through anti-dermatophytic, antioxidant, and cytotoxicity assays. ZnSm-NPs exhibited potent anti-dermatophytic activity against Microsporum canis and Trichophyton rubrum, completely inhibiting mycelial growth under the tested conditions and showing minimum inhibitory concentrations (MICs) of 3.90 and 7.81 µg/mL, respectively. In addition, ZnSm-NPs displayed strong antioxidant activity, with IC₅₀ values of 9.04 ± 0.45 µg/mL (DPPH) and 3.61 ± 0.03 µg/mL (ABTS), significantly outperforming the crude aqueous extract. Cytotoxicity assessment using Artemia salina nauplii revealed no significant acute toxicity under the tested conditions. Overall, this study demonstrates that S. muticum can be successfully valorized through green nanotechnology to produce ZnO nanoparticles with promising anti-dermatophytic and antioxidant properties. These findings highlight the potential of algal-mediated ZnO nanoparticles as sustainable bioactive nanomaterials while contributing to the valorization of invasive marine biomass.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148811768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Evaluation of Pyrazole Herbicides: QSAR-Driven Synthesis for Effective Weed Control and Crop Safety in Rice.","authors":"Kailashpati Tripathi, Rakesh Kumar, Parshant Kaushik, Virendra Singh Rana, Rajesh Kumar, T K Das, Najam Akhtar Shakil","doi":"10.1007/s12010-026-05877-6","DOIUrl":"https://doi.org/10.1007/s12010-026-05877-6","url":null,"abstract":"<p><p>A total of twenty pyrazole derivatives were synthesized using alkoxy or halo acetophenones in reaction with N, N-dimethylformamide dimethylacetal (DMF-DMA). These compounds were characterized through ¹H NMR, ¹³C NMR, and LC-HRMS techniques. Their herbicidal efficacy against barnyard grass (Echinochloa crus-galli) was evaluated via in vitro bioassays. Notably, compound 6a (5-Methoxy-2-(1H-pyrazol-3-yl) phenol) and compound 6s (3-(4-Fluoro-2-hydroxyphenyl) pyrazole) demonstrated inhibition rates of 30.6% to 54.1% and 26.9% to 50.6%, respectively, across concentrations ranging from 62.5 to 1000 µg mL^- 1. In in vivo assessments, compound 6a yielded the highest weed control index (WCI) of 51.32%, followed by compound 6s with a WCI of 50.24%. These findings indicate that compound 6a represents a promising herbicidal agent, effectively managing barnyard grass while exhibiting tolerance toward rice seedlings following both post-emergence and soil application. Toxicity predictions for the synthesized compounds were evaluated for fish (LC₅₀: 0.0001 to 38.1 mg/L), daphnids (LC₅₀: 0.0001 to 27.7 mg/L), and green algae (EC₅₀: 0.0001 to 1.70 mg/L), including chronic toxicity (ChV). Using the ECOSAR model, the assessment found that shorter chain compounds are within acceptable limits. The binding free energy of compound 6a with HPPD was relatively low (-8.3), which was consistent with the findings of bioassay activity studies. As a result, compound 6a could serve as the starting point for the development of novel HPPD inhibitors.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148786582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theranostic Smart Bionanosensors for Solid Tumors: Advances, Challenges, and Future Directions.","authors":"Manar T El-Morsy, Youssef Basem, Amr E Ali, Abanoub Sherif, Alamer Ata, Fayek Sabry, Nourhan M ElMahsoub, Ahmed S Doghish, Gharieb S El-Sayyad, Gomaa A M Ali","doi":"10.1007/s12010-026-05874-9","DOIUrl":"https://doi.org/10.1007/s12010-026-05874-9","url":null,"abstract":"<p><p>Theranostic smart bionanosensors represent a revolutionary approach to solid tumor management, integrating accurate diagnostic functions with focused therapeutic strategies. This review thoroughly analyzes the progress of biosensors from traditional diagnostic tools to multifunctional theranostic systems, highlighting advancements in nanomaterials, surface functionalization, and tumor-targeting techniques that improve imaging contrast, payload delivery, and controlled release. Advanced bionanosensors comprising optical, electrochemical, magnetic, hydrogel-based, stimuli-responsive, and wearable platforms facilitate precise biomarker detection, real-time therapeutic monitoring, and minimally invasive diagnostics, presenting unmatched prospects for personalized cancer management. Despite these advancements, challenges persist, including inconsistent tumor uptake, prolonged biocompatibility, possible toxicity, scalability, reproducibility, and regulatory obstacles. The integration of nanomaterial studies, imaging, sensor technology, computational analytics, and clinical innovation offers a strategic framework for advancing smart theranostic bionanosensors from laboratory to clinical application, facilitating precision oncology and enhancing patient outcomes.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148786722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}