Applied Biochemistry and Biotechnology最新文献

{"title":"Ethanol Fermentation-based Valorization of Alginate Extraction Residue from Pelagic Brown Seaweed Sargassum siliquosum.","authors":"Kavinilavu Rajendran, Dillirani Nagarajan, Chiu-Wen Chen, Jo-Shu Chang, Cheng-Di Dong","doi":"10.1007/s12010-026-05933-1","DOIUrl":"https://doi.org/10.1007/s12010-026-05933-1","url":null,"abstract":"<p><p>Brown seaweeds are the primary industrial source of alginates and are increasingly valued as marine-based feedstocks in integrated biorefineries. However, the substantial solid residue generated in the alginate extraction industry remains underexplored as a secondary source of carbon and energy. This study investigated the potential of the alginate-extracted residue (AER) from Sargassum siliquosum biomass collected from the southwestern coast of Taiwan as a substrate for bioethanol production. The alkaline extraction method yielded 39.00% alginate and 33.80% residue on a dry weight basis. Comprehensive characterization of the AER revealed that the solid fraction is enriched in carbohydrates (33.00%), especially the cellulose fraction, with a monosaccharide composition of glucose, fucose, and xylose in addition to guluronic acid. Elemental analysis revealed that the residue is enriched in carbon (35.29%) and shows notable reductions in ash, sulfur, and phenolic compounds. Fourier-transform infrared spectroscopy (FT-IR) analysis confirmed the selective removal of mannuronic and guluronic acid residues in AER compared to biomass, while cellulose-associated functional groups have been preserved. Enzymatic hydrolysis with commercial fungal cellulase released 2.90 g/L glucose from biomass and 5.10 g/L glucose from AER, respectively. Fermentation of the hydrolysates with Saccharomyces cerevisiae BCRC 22458 yielded ethanol titers of 0.89 and 1.20 g/L from biomass and AER hydrolysate, respectively. Concentration of the hydrolysates to increase the sugar concentration increased the ethanol titer to 2.47 g/L (yield 0.098 g/g biomass; productivity 0.33 g/L/h) for AER and 1.18 g/L for biomass. These results demonstrated that S. siliquosum AER is a promising secondary feedstock for bioethanol fermentation within a cascading biorefinery approach for brown seaweeds.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaoyao Decoction Inhibits Colorectal Cancer Progression by Targeting PD-L1 to Reprogram the Immunosuppressive Tumor Microenvironment.","authors":"Xiaoyan Wang, Zhuoga Mima, Wujie Sai, Lijuan Song, Hao Li","doi":"10.1007/s12010-026-05926-0","DOIUrl":"https://doi.org/10.1007/s12010-026-05926-0","url":null,"abstract":"<p><p>Colorectal cancer (CRC), particularly the microsatellite-stable (MSS) subtype accounting for ~ 85% of cases, remains refractory to immune checkpoint inhibitors due to persistent immunosuppression. Shaoyao Decoction (SYD), a traditional Chinese herbal formula, is known for its anti-inflammatory and anticancer effects. However, the mechanisms by which it modulates immune responses in colorectal cancer (CRC) remain poorly understood. Here, we evaluated SYD's efficacy in AOM/DSS-induced colitis-associated CRC and PD-L1-overexpressing MC38 syngeneic models, using the PD-1/PD-L1 inhibitor BMS-202 as a positive control. In vivo, SYD significantly suppressed tumor growth, normalized colon structure, and reduced tumor burden, with efficacy comparable to BMS-202 but without overt toxicity. Mechanistically, SYD downregulated PD-L1 expression in tumor and spleen tissues, shifted serum cytokines toward a pro-inflammatory profile characterized by increased IL-2 and IFN-γ as well as decreased IL-10 and TGF-β, and restored CD4⁺/CD8⁺ T cell balance. In vitro, SYD inhibited the viability of PD-L1-overexpressing MC38 cells and reduced PD-L1 mRNA expression. Collectively, SYD exerts potent anti-CRC effects by targeting PD-L1 to remodel the immunosuppressive tumor microenvironment. As a multi-target agent, it holds great promise for MSS-CRC, bridging TCM with modern immuno-oncology.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Escherichia coli Nissle 1917 for the highly efficient biosynthesis of food-grade ergothioneine.","authors":"Yuwen Tu, Zhaoyi Luo, Kuiyi Chen, Weirui Zhao, Sheng Hu, Peng Lv, Yu Deng, Lehe Mei, Changjiang Lyu","doi":"10.1007/s12010-026-05932-2","DOIUrl":"https://doi.org/10.1007/s12010-026-05932-2","url":null,"abstract":"<p><p>Ergothioneine (EGT) is a natural sulfur-containing amino acid possessing potent antioxidant activity. It demonstrates a range of beneficial biological functions, including anti-inflammatory, neuroprotective, and cardioprotective effects. Microbial fermentation presents a promising alternative to traditional extraction from edible fungi for EGT production, offering greater scalability, though its feasibility hinges critically on production titer and cost. In this study, to achieve food-grade EGT synthesis, the probiotic Escherichia coli Nissle 1917 was employed as the host, and the heterologous enzymes methyltransferase EgtD and C-S lyase EgtE from Mycobacterium, as well as the bifunctional enzyme Egt1 from Neurospora crassa, were introduced. As a result, shake flask fermentations with the designed strain E1 achieved an EGT titer of 109.2 mg/L. Subsequently, overexpression of the EgtE mutant EgtE^Y106F along with the incorporation of the protein fusion tag NT11 at the N-terminal via a dual-plasmid system increased the yield to 159.9 mg/L. Furtherly, the cellular metabolic homeostasis regulator RpoS was introduced and placed under the control of an arabinose-inducible promoter, thus achieving an EGT titer of 429.2 mg/L for the engineered strain E14 within 24 h of shake flask fermentation. Finally, fed-batch fermentation conducted in a 5-L bioreactor resulted in an EGT titer of 12.3 g/L at 120 h, representing the highest level reported to date. This study developed a scalable and cost-effective platform for food-grade EGT production, paving the way for its industrial application.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear Import of E2F1 Regulated by KPNB1 is Essential for Proliferation and Invasion of Cervical Cancer Cells.","authors":"Mengjie Zhang, Jing Hai, Jing Yang, Ying Zhang, Xuecai Dong","doi":"10.1007/s12010-026-05928-y","DOIUrl":"https://doi.org/10.1007/s12010-026-05928-y","url":null,"abstract":"<p><p>As a nuclear transport receptor, KPNB1 transport proteins from cytoplasm into nucleus, performing a vital function in intracellular traffic. The aberrant upregulation of KPNB1 has been associated with various malignancies and transformed cells, where it is indispensable for cellular proliferation and survival. Transcription factors like E2F1 possess a nuclear localization motif that triggers the expression of a range of crucial factors during tumorigenesis. This investigation explores the influence of KPNB1 in cervical cancer (CC) cell metastasis and proliferation through the modulation of E2F1 nuclear import. Overexpression of KPNB1 was consistently detected in cervical carcinoma tissues and associated cell lines, with notably higher levels observed relative to normal cervical mucosa. Functional studies demonstrated that KPNB1 silencing induced a marked attenuation in the invasive, migratory, and proliferative capacities of HeLa and SiHa cells. Furthermore, inhibition of KPNB1 hindered the nuclear translocation of E2F1. Importantly, the application of ARQ171, a small-molecule compound that functionally elevates E2F1 expression, negated partially the suppressive effects of KPNB1 on migration, invasion, and growth of CC cells via elevating the total amount of nuclear E2F1, suggesting that E2F1 mediates the oncogenic role of KPNB1 in CC progression. The research findings highlight the potential utility of targeting KPNB1-E2F1 axis as an innovative treatment modality, underscoring its promise as a novel approach in cervical cancer treatment.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification, Characterization, and In Vitro Safety Assessment of the Antimicrobial Peptide SAM-KB40 from Bacillus licheniformis SAM-D318","authors":"En Liu,&nbsp;Weiping Huo,&nbsp;Fuli Xu,&nbsp;Xing Liu,&nbsp;Yan Liu,&nbsp;Chunqiao Shan,&nbsp;Qiuchen Liu,&nbsp;Mingchun Liu,&nbsp;Guotuo Jiang","doi":"10.1007/s12010-026-05940-2","DOIUrl":"10.1007/s12010-026-05940-2","url":null,"abstract":"<div><p>Antimicrobial peptides (AMPs) are a diverse class of bioactive molecules that exert their effects through multiple mechanisms, including membrane disruption, inhibition of cell wall synthesis, interference with intracellular targets, and binding to nucleic acids or proteins. They exhibit broad-spectrum antimicrobial activity and a low propensity to induce drug resistance, positioning them as promising candidates for combating multidrug-resistant infections. However, their clinical translation faces significant challenges, including protease susceptibility, potential cytotoxicity, high production costs, and suboptimal pharmacokinetic properties. In this study, a strain of <i>Bacillus licheniformis</i> SAM-D318 isolated from Hu sheep intestine was used as the peptide-producing strain; genomic analysis confirmed the absence of classical virulence toxin genes despite intrinsic multidrug resistance. A previously unreported cationic α-helical antimicrobial peptide, SAM-KB40 (4176.92 Da, pI 10.886), was obtained from the fermentation supernatant of <i>B. licheniformis</i> SAM-D318 and purified using cation-exchange chromatography and C18 reversed-phase chromatography, achieving a 70.45-fold increase in specific activity. Structural prediction by PEP-FOLD4 suggested an α-helical propensity in the C-terminal region, while circular dichroism spectroscopy indicated that SAM-KB40 adopts a predominantly random coil conformation in aqueous solution. The peptide exhibited potent antibacterial activity against <i>Clostridium perfringens</i> (MIC = 0.5 µg/mL, MBC = 4 µg/mL) and showed selective inhibition against <i>Staphylococcus aureus</i> and <i>Listeria monocytogenes</i>. SAM-KB40 demonstrated exceptional thermal stability (&gt; 97% activity retained at 100 °C for 60 min), good tolerance to acidic conditions (pH 3–4) and bile salts (up to 0.50%), and remarkably low hemolytic activity (1.96% at 1000 µg/mL). Notably, the peptide neutralized recombinant <i>C. perfringens</i> α-toxin at 16 µg/mL in a Vero cell-based assay and exhibited no cytotoxicity at concentrations up to 128 µg/mL, demonstrating a favorable in vitro safety profile.Sequence homology analysis against UniProt, NCBI, APD, and BACTIBASE databases confirmed that SAM-KB40 has not been previously reported. While the peptide shows promising in vitro dual antibacterial and toxin-neutralizing capacity, its high sensitivity to proteinase K (39.78% residual activity) limits oral application. These findings position SAM-KB40 as a promising lead candidate for further development as a feed additive or therapeutic agent against <i>C. perfringens</i>-associated diseases, pending subsequent in vivo validation.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 11","pages":"8761 - 8792"},"PeriodicalIF":3.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12010-026-05940-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Differential Detection of PRRSV NADC30-like and NADC34-like Strains Using a CRISPR-Cas13a/RT-RAA Assay","authors":"Hao Wang,&nbsp;Xinwei Wang,&nbsp;Qinghua Geng,&nbsp;Miao Li,&nbsp;Zhuo Li,&nbsp;Jinling Liu,&nbsp;Zuofeng Yang,&nbsp;Desong Lan,&nbsp;Zeliang Chen","doi":"10.1007/s12010-026-05916-2","DOIUrl":"10.1007/s12010-026-05916-2","url":null,"abstract":"<div><p>The global swine industry suffers substantial economic losses from Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), which causes reproductive and respiratory disorders. With NADC30- and NADC34-like strains now predominant, differentiation is complicated by their similar biology, despite characteristic Nsp2 gene deletions of 131 aa and 100 aa, respectively, highlighting a need for improved diagnostics. Moving fundamentally beyond conventional broad-spectrum detection, this assay innovatively targets the characteristic Nsp2 deletion signatures. A diagnostic platform integrating CRISPR-Cas13a with recombinase-aided amplification (RT-RAA) was constructed. Utilising aligned Nsp2 sequences, conserved crRNAs, and RT-RAA primers, NADC30- and NADC34-like strains were evaluated for their assay time, specificity, sensitivity, and consistency with RT-qPCR. The assay achieved rapid, specific detection with no cross-reactivity to common swine pathogens, and highly sensitive detection limits of 1.7 × 10¹ and 2.3 × 10¹ copies/µL for NADC30- and NADC34-like strains, respectively. Clinical validation using 30 samples demonstrated complete concordance with RT-qPCR, and the integration of T7 transcription enabled the single-tube differentiation of both strains. In summary, this one-tube, two-step fluorescent system presents a robust alternative for PRRSV diagnostics, combining specificity, sensitivity, and ease of use. It is well-suited for rapid field identification, molecular typing, and surveillance, thereby aiding in the implementation of targeted control measures.</p></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 11","pages":"9312 - 9328"},"PeriodicalIF":3.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RBM15 Contributes to Bladder Carcinoma Progression via m6A-dependent Stabilization of IFI6 mRNA.","authors":"Hanchao Zhang, Yue Yang, Zhengdao Liu, Faliang Zhao, Guobiao Liang","doi":"10.1007/s12010-026-05924-2","DOIUrl":"https://doi.org/10.1007/s12010-026-05924-2","url":null,"abstract":"<p><p>Bladder carcinoma (BLCA) is a prevalent urological malignancy with limited therapeutic options. Interferon α inducible protein 6 (IFI6) is implicated in various cancers, but its role in BLCA remains unexplored. qRT-PCR and western blot were used for expression analysis. Functional assays (cell counting kit 8, 5-ethynyl-2'-deoxyuridine staining, flow cytometry, wound healing, transwell, and 2', 7'-dichlorodihydrofluorescein diacetate fluorescent probe) and xenograft mouse models were used to assess the effects of IFI6 and RNA binding motif protein 15 (RBM15) on BLCA. The N6-methyladenosine (m6A) modification sites on IFI6 were predicted by the sequence-based RNA adenosine methylation site predictor website and the combination of RBM15 was validated via RNA-binding proteins site prediction websites, methylated RNA immunoprecipitation, Actinomycin D, dual-luciferase reporter assay, and RNA binding protein immunoprecipitation assays. IFI6 was elevated in BLCA tissues and cells. IFI6 knockdown could inhibit proliferation, migration, and invasion, while enhancing apoptosis and oxidative stress of BLCA cells. Besides, silencing of IFI6 blocked BLCA tumor growth. RBM15, an m6A writer, directly bound IFI6 to stabilize its mRNA through m6A modification. Rescue experiments confirmed that IFI6 overexpression reversed RBM15 knockdown-mediated the suppression effect on BLCA cell proliferation and metastasis. Our study identifies that RBM15/IFI6 axis is a novel m6A-dependent pathway driving BLCA progression, offering a potential therapeutic target for BLCA.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Dipeptides Enhance Stress Tolerance and Fermentation Performance of Lager Yeast During High-Gravity Brewing","authors":"Caiyun Wu,&nbsp;Yingying Huo,&nbsp;Shuyuan Zhang,&nbsp;Hongjie Lei","doi":"10.1007/s12010-026-05936-y","DOIUrl":"10.1007/s12010-026-05936-y","url":null,"abstract":"<div><p>This study aimed to evaluate the effects of antioxidant dipeptides (AH, TY, and FC) on yeast stress tolerance, beer physicochemical properties, and sensory quality during high-gravity brewing. The results demonstrated that antioxidant dipeptides maintained yeast cell morphology and alleviated cell shrinkage. Additionally, the dipeptides decreased intracellular reactive oxygen species (ROS) levels, with AH and FC exhibiting the most significant reductions of 9.69% and 11.01%, respectively, compared to the control. Moreover, antioxidant dipeptides significantly increased the ethanol content and wort fermentability of the beer. Beer aroma analysis revealed that dipeptide supplementation enhanced both the content and diversity of ester compounds without adversely affecting beer flavor. Therefore, antioxidant dipeptides represent a promising additive for enhancing the stress tolerance and fermentation performance of lager yeast during high-gravity brewing.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 11","pages":"8644 - 8662"},"PeriodicalIF":3.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Reusable Na[DMSH] Ionic Liquid for Corn Cob Pretreatment: Structural Characterization and Enhanced Enzymatic Hydrolysis","authors":"Manita Kuntapa,&nbsp;Chakrit Tachaapaikoon,&nbsp;Pichet Ninduangdee,&nbsp;Kaokanya Sudaprasert","doi":"10.1007/s12010-026-05896-3","DOIUrl":"10.1007/s12010-026-05896-3","url":null,"abstract":"<div><p>This study investigated the synthesis, reusability, and pretreatment performance of sodium dimethyl sulfoxide (Na[DMSH]) for corn cob biomass pretreatment and subsequent enzymatic hydrolysis. The chemical characteristics of the Na[DMSH] system were evaluated by NMR, while the retention of sodium and DMSO during repeated reuse was quantitatively monitored using ICP-OES and GC analysis, respectively. The pretreated corn cob was subsequently subjected to enzymatic hydrolysis, and the hydrolysis products were analyzed by LC–MS/MS using multiple reaction monitoring (MRM). Cellobiose was confirmed by comparison with an authentic standard based on retention time, precursor ion, and product ions. After 16 reuse cycles, the solid lignin content was 6.69 ± 0.27%, compared with 6.45 ± 0.14% for the non-reused Na[DMSH] treatment, while cellulose conversion remained high at 87.60 ± 0.13% and 88.43 ± 0.18%, respectively. The Na and DMSO retention after 16 cycles were 96.0% and 77.9%, respectively, indicating that the Na[DMSH]:DMSO system retained substantial chemical components during repeated use. LC–MS/MS analysis confirmed the presence of cellobiose, while HMF, furfural, and levulinic acid were not detected under the analytical conditions used. Overall, the results demonstrate that Na[DMSH] can be repeatedly reused for corn cob pretreatment while maintaining high delignification performance and enzymatic cellulose conversion. The findings provide experimental evidence supporting the potential of the Na[DMSH] system as a reusable pretreatment medium for lignocellulosic biomass.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"198 11","pages":"8414 - 8441"},"PeriodicalIF":3.5,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148981737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Padina boergesenii-Derived Zinc Oxide Nanoparticles and their Therapeutic Potential Against Oral Squamous Cell Carcinoma: A Transcriptomic and in Vitro Evaluation.","authors":"Ashok Kumar, Sheikh F Ahmad, Sabry M Attia, S Winkins Santosh, Uday Aditya Sarkar, R Magesh, Rashu Barua, Shiek Ssj Ahmed","doi":"10.1007/s12010-026-05925-1","DOIUrl":"https://doi.org/10.1007/s12010-026-05925-1","url":null,"abstract":"<p><p>Cancer remains a major health challenge, with oral squamous cell carcinoma (OSCC) being an high aggressive subtype of head and neck squamous cell carcinoma that lacks effective therapeutic options. Current study integrates the synthesis of zinc oxide nanoparticles (ZnO-NPs) from the marine brown algae Padina boergesenii with the OSCC gene expression profile to evaluate their potential therapeutic effects against OSCC. Herein, the ZnO-NPs from Padina boergesenii were prepared through the green synthesis method. The obtained ZnO-NPs were characterized through spectroscopic methods, the UV spectrophotometer shows maximum absorbance at 372 nm, FT-IR presents Zn-O functional band at 516 cm^- 1, HR-TEM confirms average particle size of 55.70 nm and the Zetasizer shows zeta potential of + 12.9 mV, indicating colloidal stability. The cytotoxicity assay with ZnO-NPs against oral cancer cell lines exhibited a reduction in cell viability at IC₅₀ value of 20 µg/mL. Meanwhile, the transcriptome analysis of OSCC highlights that MYC, STAT3, BRCA1, and AKT1 are the OSCC therapeutic targets involved in proliferation, immune evasion, genomic instability, and cancer signalling pathways. Further, qRT-PCR based gene expression analysis demonstrates significant down-regulation of these targets upon ZnO-NPs treatment in KB cell lines. Overall, this study emphasizes the anticancer potential of Padina boergesenii-derived ZnO-NPs that could effectively modulate the therapeutic targets and may benefit the treatment of OSCC cancer.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148981783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}