Applied Biochemistry and Biotechnology最新文献_第4页

Injectable Silver Nanoparticle-Based Hydrogel Dressings with Rapid Shape Adaptability and Antimicrobial Activity 具有快速形状适应性和抗菌活性的注射型纳米银水凝胶敷料

IF 3 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-09-10 DOI: 10.1007/s12010-024-05048-5

Yuanyuan Zhao, Zhaobo Dai, Huimin Huang, Jia Tian, Haibo Cai

{"title":"Injectable Silver Nanoparticle-Based Hydrogel Dressings with Rapid Shape Adaptability and Antimicrobial Activity","authors":"Yuanyuan Zhao, Zhaobo Dai, Huimin Huang, Jia Tian, Haibo Cai","doi":"10.1007/s12010-024-05048-5","DOIUrl":"https://doi.org/10.1007/s12010-024-05048-5","url":null,"abstract":"Burns and scalds often result in deep wounds that challenge adequate debridement and inflammation control using traditional sheet-like hydrogel dressings. Herein, we developed an antibacterial, injectable, and self-healing hydrogel (ADCM@Ag) by employing carboxymethyl chitosan (CMCS) for in situ green reduction of silver ions and utilizing a spontaneous Schiff base reaction with aldehyde-functionalized dextran (AD). SEM analysis revealed a porous structure within the hydrogel. Swelling and enzymatic degradation assays demonstrated that ADCM@Ag hydrogel possesses excellent fluid absorption capacity and biodegradability. Mechanical tests indicated good mechanical properties, allowing the hydrogel to withstand external forces when applied to animal wounds. The hydrogel exhibited good injectability, shape adaptability, and self-healing capability. Cell experiments showed that the ADCM@Ag hydrogel avoided the cytotoxicity caused by high concentrations of silver ions and had good cell compatibility. Antimicrobial assays showed that ADCM@Ag exhibited potent bactericidal effects against Gram-negative and Gram-positive bacteria, achieving at least 85% killing efficacy. Collectively, ADCM@Ag hydrogel has good potential for wound dressing applications.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142227561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Nonlinear Reaction-Diffusion in Enzyme Immobilized Systems: Integer and Fractional Order Modeling. 探索酶固定化系统中的非线性反应-扩散：整数阶和分数阶建模。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-09-06 DOI: 10.1007/s12010-024-05050-x

R Rajaraman

{"title":"Exploring Nonlinear Reaction-Diffusion in Enzyme Immobilized Systems: Integer and Fractional Order Modeling.","authors":"R Rajaraman","doi":"10.1007/s12010-024-05050-x","DOIUrl":"https://doi.org/10.1007/s12010-024-05050-x","url":null,"abstract":"This paper presented a kinetic model of the Langmuir-Hinshelwood-Hougen-Watson (LHHW) type for porous catalysts with simple one-dimensional geometry, including spheres, infinite cylinders, and flat pellets. The model was applied to systems involving immobilized enzymes, where enzymes are attached to porous support materials to enhance stability and reusability. The LHHW model provided a tool for understanding and modeling reaction kinetics in heterogeneous porous catalysts and immobilized enzymes. A nonlinear reaction-diffusion equation was generated using finite-range Fickian diffusion and nonlinear reaction kinetics, crucial for accurately modeling the behavior of immobilized enzymes. This research addressed a gap in the existing literature by introducing fractional derivatives to investigate enzyme reaction kinetics, capturing the complex dynamics of substrate interaction and reaction rates within the porous matrix. An approximation method based on Lucas wavelets was employed to find solutions for substrate concentration and effectiveness factors across various parameter values. The analytical solutions derived from the Lucas wavelet method (LWM) were evaluated against the fourth-order Runge-Kutta method, showing great agreement between the LWM solutions and numerical counterparts. These results optimized diffusion and reaction kinetics, paving the way for advancements in biocatalysis and efficient enzyme reactor design.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142138929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical Characterization of Hyaluronate Lyase CpHly8 from an Intestinal Microorganism Clostridium perfringens G1121. 来自肠道微生物产气荚膜梭菌 G1121 的透明质酸溶解酶 CpHly8 的生化特性。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-09-05 DOI: 10.1007/s12010-024-05025-y

Yongqing Fu, Zheng Fu, Jing Yu, Hainan Wang, Yuzhu Zhang, Mei Liu, Xiaolei Wang, Wengong Yu, Feng Han

{"title":"Biochemical Characterization of Hyaluronate Lyase CpHly8 from an Intestinal Microorganism Clostridium perfringens G1121.","authors":"Yongqing Fu, Zheng Fu, Jing Yu, Hainan Wang, Yuzhu Zhang, Mei Liu, Xiaolei Wang, Wengong Yu, Feng Han","doi":"10.1007/s12010-024-05025-y","DOIUrl":"https://doi.org/10.1007/s12010-024-05025-y","url":null,"abstract":"Hyaluronic acid (HA) is an important component of extracellular matrices (ECM) and a linear polysaccharide involved in various physiological and pathological processes within the biological system. Several pathogens exploit HA degradation within the extracellular matrix to facilitate infection. While many intestinal microorganisms play significant roles in HA utilization in the human body, there remains a scarcity of related studies. This paper addressed this gap by screening intestinal microorganisms capable of degrading HA, resulting in the isolation of Clostridium perfringens G1121, which had been demonstrated the ability to degrade HA. Subsequent genome sequencing and analysis of C. perfringens G1121 revealed its utilization of the polysaccharide utilization loci of HA (PULHA), which was obtained by horizontal gene transfer. The PULHA contains a sequence encoding a hyaluronic acid-specific degradation enzyme designated CpHly8, belonging to polysaccharide lyase family 8. The specific activity of CpHly8 towards HA was 142.98 U/mg, with the optimum reaction temperature and pH observed at 50℃ and 6.0, respectively. The final product of HA degradation by CpHly8 was unsaturated hyaluronic acid disaccharide. Moreover, subcutaneous diffusion experiments with trypan blue in mice revealed that CpHly8 effectively promoted subcutaneous diffusion and sustained its effects long-term, suggesting its potential application as an adjunct in drug delivery. Overall, our study enriches our understanding of intestinal microbial degradation of HA, provides new evidence for horizontal gene transfer among intestinal microorganisms, and confirms that CpHly8 is a promising candidate for intestinal microbial hyaluronidase.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardioprotective Effects of Phlorizin on Hyperlipidemia-induced Myocardial Injury: Involvement of Suppression in Pyroptosis via Regulating HK1/NLRP3/Caspase-1 Signaling Pathway. 叶枯素对高脂血症所致心肌损伤的心脏保护作用：通过调控HK1/NLRP3/Caspase-1信号通路抑制猝死的作用

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-09-03 DOI: 10.1007/s12010-024-05056-5

Yuling Zhang, Yanan Wang, Xizhen Cheng, Haochuan Guo, Donglai Ma, Yongxing Song, Yajing Zhang, Hongfang Wang, Huiru Du

{"title":"Cardioprotective Effects of Phlorizin on Hyperlipidemia-induced Myocardial Injury: Involvement of Suppression in Pyroptosis via Regulating HK1/NLRP3/Caspase-1 Signaling Pathway.","authors":"Yuling Zhang, Yanan Wang, Xizhen Cheng, Haochuan Guo, Donglai Ma, Yongxing Song, Yajing Zhang, Hongfang Wang, Huiru Du","doi":"10.1007/s12010-024-05056-5","DOIUrl":"https://doi.org/10.1007/s12010-024-05056-5","url":null,"abstract":"Hyperlipidemia (HLP) is a prevalent and intricate condition that plays a pivotal role in impairing heart function. The primary objective of this study was to assess the lipid-lowering and cardioprotective properties of phlorizin (PHZ) and to investigate its potential molecular mechanisms in rats. In this investigation, Sprague-Dawley rats were subjected to a high-fat diet for a period of 28 days to induce an HLP model. Subsequently, the rats received oral doses of PHZ or metformin from day 14 to day 28. We assessed various parameters using commercially available kits, including serum lipid deposition, myocardial injury biomarkers, oxidative stress markers, and inflammatory cytokine levels. We also employed electron microscopy to examine myocardial ultrastructural changes and conducted Western blot analyses to assess apoptosis factors and pyroptosis markers. Comparing the PHZ group with the model group, we observed significant improvements in blood lipid deposition and heart injury biomarkers. Furthermore, PHZ demonstrated a clear reduction in myocardial tissue oxidative stress and inflammatory factors, as well as a suppression of cell apoptosis. Subsequent investigations indicated that PHZ treatment led to a decreased inflammatory response and lowered levels of hexokinase 1 (HK1), NOD-like receptor thermal protein domain associated protein 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and Caspase-1. In summary, PHZ proved to be an effective remedy for alleviating HLP-induced cardiac damage by reducing blood lipid levels, mitigating oxidative stress, curbing inflammation, and suppressing pyroptosis. The inhibition of pyroptosis by PHZ appears to be linked to the regulation of the HK1/NLRP3/Caspase-1 signaling pathway.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142118686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Esterase (BaCE_m) Immobilized on Polyethyleneimine-Impregnated Mesoporous Silica SBA-15 Exhibits Outstanding Catalytic Performance. 固定在聚乙烯亚胺浸渍介孔二氧化硅 SBA-15 上的重组酯酶 (BaCEm) 表现出卓越的催化性能。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-09-02 DOI: 10.1007/s12010-024-05045-8

Hongjun Zhang, Yichao Li, Litian Wei, Zhao Wang, Yinjun Zhang

{"title":"Recombinant Esterase (BaCEm) Immobilized on Polyethyleneimine-Impregnated Mesoporous Silica SBA-15 Exhibits Outstanding Catalytic Performance.","authors":"Hongjun Zhang, Yichao Li, Litian Wei, Zhao Wang, Yinjun Zhang","doi":"10.1007/s12010-024-05045-8","DOIUrl":"https://doi.org/10.1007/s12010-024-05045-8","url":null,"abstract":"A recombinant esterase, BaCEm, derived from Bacillus aryabhattai and heterologously expressed in Escherichia coli, was successfully immobilized on polyethyleneimine-impregnated mesoporous silica SBA-15. This immobilization utilized glutaraldehyde as a crosslinker. Optimal conditions were established with a PEI/SBA-15 ratio of 25% (w/w), a pH of 7.5, and a glutaraldehyde concentration of 0.5% (w/w), resulting in a loading capacity of 76.4 mg/g, a recovery activity of 43.5%, and a specific activity of 7917 U/g for BaCEm. The immobilized BaCEm demonstrated high enantioselectivity, with an \"E\" value of 203.92, in the resolution assay of (R,S)-ethyl indoline-2-carboxylate. Notably, the immobilized enzyme, compared to its free counterpart, exhibited enhanced thermostability, maintaining 95.4% of its activity after 3 h at 30 °C. It also showed significant tolerance to organic solvents, retaining 48.4% and 28.7% residual activity in 10% v/v acetonitrile and acetone, respectively. Moreover, its storage stability was confirmed, with 68.5% residual activity preserved after 30 days at 4 °C. Remarkably, the immobilized BaCEm retained 58.1% of its activity after 10 reuse cycles, underscoring the potential of polyethyleneimine-impregnated mesoporous silica SBA-15 as an effective support for enzyme immobilization, promising for industrial applications.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of an Amino Acid Metabolism Reprogramming Signature for Predicting Prognosis, Immunotherapy Efficacy, and Drug Candidates in Colon Cancer. 鉴定用于预测结肠癌预后、免疫疗法疗效和候选药物的氨基酸代谢重编程特征。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-09-02 DOI: 10.1007/s12010-024-05049-4

Fenqi Du, Xiangxin Wu, Yibo He, Shihui Zhao, Mingyu Xia, Bomiao Zhang, Jinxue Tong, Tianyi Xia

{"title":"Identification of an Amino Acid Metabolism Reprogramming Signature for Predicting Prognosis, Immunotherapy Efficacy, and Drug Candidates in Colon Cancer.","authors":"Fenqi Du, Xiangxin Wu, Yibo He, Shihui Zhao, Mingyu Xia, Bomiao Zhang, Jinxue Tong, Tianyi Xia","doi":"10.1007/s12010-024-05049-4","DOIUrl":"https://doi.org/10.1007/s12010-024-05049-4","url":null,"abstract":"Colon cancer ranked third among the most frequently diagnosed cancers worldwide. Amino acid metabolic reprogramming was related to the occurrence and development of colon cancer. We looked for the amino acid metabolism genes (AMGs) associated with amino acid metabolism from molecular signatures database as prognostic markers and constructed amino acid metabolism scoring model (AMS). According to AMS, the patients were divided into high AMS and low AMS groups, and the prognostic characteristics, molecular phenotypes, somatic cell mutation characteristics, immune cell infiltration characteristics, and immunotherapy effect of the two groups were systematically analyzed. Finally, the compounds targeting AMGs were also screened. We screen out 6 prognostic AMGs (P < 0.05) and construct an AMS model based on them. K-M curve indicated that OS in low AMS group was significantly higher than that in high group (P < 0.05), which were validated in multiple datasets. And different AMS groups had different molecular phenotypes, somatic cell mutation characteristics and immune cell infiltration characteristics. Low AMS group had a better effect for immunotherapy. In addition, we predicted potential therapeutic compounds that could bind to AMGs target proteins. AMS model can be used as a hierarchical tool to evaluate the prognosis, immune infiltration characteristics and immunotherapy response ability of colon cancer. And the compounds screened based on AMGs may become new anti-tumor drugs.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Review: Versatile Imaging Probe Based on Chemical Materials for Biomedical Applications. 全面回顾：基于化学材料的生物医学应用多功能成像探针。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-31 DOI: 10.1007/s12010-024-05043-w

Rui Wu, Guanghui Tian, Shengrui Zhang, Pengfei Zhang, Xiaoyun Lei

引用次数: 0

Organ-Specific Metabolomics of Aerial Parts of Melochia corchorifolia for Antibiofilm and Wound Healing Activities. Melochia corchorifolia 药用部位器官特异性代谢组学研究抗生物膜和伤口愈合活性。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-31 DOI: 10.1007/s12010-024-05053-8

Shunmugiah Mahendran, Vinayagamoorthy Mohanapriya, Jeyaraj John Wilson, Debasish Swain, Vaikundamoorthy Ramalingam

{"title":"Organ-Specific Metabolomics of Aerial Parts of Melochia corchorifolia for Antibiofilm and Wound Healing Activities.","authors":"Shunmugiah Mahendran, Vinayagamoorthy Mohanapriya, Jeyaraj John Wilson, Debasish Swain, Vaikundamoorthy Ramalingam","doi":"10.1007/s12010-024-05053-8","DOIUrl":"https://doi.org/10.1007/s12010-024-05053-8","url":null,"abstract":"Melochia corchorifolia is a well-known perennial herb and has been used in traditional medicine for the treatment of a wide number of diseases. However, the phytochemical investigation in the different organs of the M. corchorifolia was poorly understood. In the present study, the organ-specific metabolomic profiling of leaves, stems, and vegetable extract of M. corchorifolia was determined, and their potential antibiofilm activity with wound healing properties was evaluated. The UPLC-ESI-Q-TOF-MSE analysis showed 59 compounds in the leaf, stem, and vegetable extracts of M. corchorifolia. The crystal violet staining assay clearly showed that the extracts of M. corchorifolia have excellent antibiofilm activity against Proteus mirabilis and Salmonella typhi. The extracts of M. corchorifolia also caused the architecture of the bacterial biofilm by inhibiting the adherence to polystyrene and auto-aggregation and subsequently inhibiting the growth and colonization of the biofilm-forming bacteria P. mirabilis and S. typhi. The extracts of M. corchorifolia accelerate the wound healing process in BALB/c mice by completely closing the wound on the 20th day of treatment. Together, the phytochemicals present in the leaf, stem, and vegetable extracts of M. corchorifolia are responsible for potent antibiofilm and wound healing properties and could be used as an excellent remedy for treating chronic wounds and their associated infectious disease.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impacts of Cellulose on Volatile Fatty Acid Production and the Microbial Community in Anaerobic Fermentation of Sludge at High and Medium Temperatures. 中高温污泥厌氧发酵中纤维素对挥发性脂肪酸产量和微生物群落的影响

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-30 DOI: 10.1007/s12010-024-05052-9

Suyu Li, Yilong Yin, Ruyan Zhang, Chongyang Wang

{"title":"The Impacts of Cellulose on Volatile Fatty Acid Production and the Microbial Community in Anaerobic Fermentation of Sludge at High and Medium Temperatures.","authors":"Suyu Li, Yilong Yin, Ruyan Zhang, Chongyang Wang","doi":"10.1007/s12010-024-05052-9","DOIUrl":"https://doi.org/10.1007/s12010-024-05052-9","url":null,"abstract":"During large-scale sewage treatment, a large amount of excessive sludge is produced, which will cause serious pollution in the environment. In recent years, anaerobic digestion technology has been widely promoted because it can achieve better sludge reduction, and the products and byproducts after anaerobic digestion can be fully utilized as resources. In this study, cellulose was added as the co-fermentation substrate during the fermentation process at 30 ℃ and 50 ℃ to enhance the production of VFAs. The result indicated that cellulose could significantly increase the yield of VFAs in both 30 ℃ and 50 ℃. Meanwhile, COD and reducing sugar generation in the fermentation process were also measure. Analysis of the microbial community structure at the class and genus levels revealed that the proportion of several genus closely related with cellulose degradation such as Cellvibrio, Fibrobacter, and Sporocytophaga were significantly increased with the addition of cellulose. Co-fermentation was recognized as an economic and environmental friendly strategy for sludge and other solid waste treatment. The analysis of the effect of cellulose as a substrate on the production of VFAs at high and medium temperatures is highly important for exploring ways to increase the production of VFAs in anaerobic fermentation.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the Quality of Low-grade Tobacco by Enzymatic Treatment and Co-fermentation with Yeast and Lactic Acid Bacteria. 通过酶处理以及酵母和乳酸菌共同发酵提高低等级烟草的质量。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2024-08-29 DOI: 10.1007/s12010-024-05007-0

Wei Zhang, Qian Deng, Baokun Zhu, Dong Xiao, Qiuming Chen, Hongyang Pan, Jie Chen

{"title":"Improving the Quality of Low-grade Tobacco by Enzymatic Treatment and Co-fermentation with Yeast and Lactic Acid Bacteria.","authors":"Wei Zhang, Qian Deng, Baokun Zhu, Dong Xiao, Qiuming Chen, Hongyang Pan, Jie Chen","doi":"10.1007/s12010-024-05007-0","DOIUrl":"https://doi.org/10.1007/s12010-024-05007-0","url":null,"abstract":"Enzymatic treatment is a promising method to modulate the chemical composition, flavoring substances and to enhance the sensory quality of cigarettes. This study investigates the feasibility of enzymatic treatment in conjunction with co-fermentation with yeast and lactic acid bacteria to improve the quality of low-quality cigarettes. Amylase, flavourzyme, glucoamylase, protease, and their combinations were used for the enzymatic treatment of tobacco-sorghum by using the liquid-state fermentation method. The biochemical components and flavor substances of the fermented products were analyzed. The findings show that amylase and glucoamylase can effectively degrade starch into fermentable reducing sugars, facilitate microbial growth and proliferation, and significantly enhance the levels of flavoring alcohols and esters. Flavourzyme and glucoamylase, either individually or combined with amylase, achieved a more balanced distribution of flavor substances in the products. Additionally, flavourzyme was capable of increasing the content of guaiacol, solanesol, and 2-acetylpyrrole in the fermented products, thereby improving the richness and depth of the cigarette flavor. While the protease treatment group showed fewer flavor substances and higher nicotine content, which was detrimental to the quality of cigarettes. When integrating the fermentation products into low-quality cigarettes, flavourzyme and glucoamylase combined with amylase treatment received higher scores in sensory quality evaluations. This study provides a beneficial strategy for effectively improving the quality of low-quality cigarettes.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0