Applied Biochemistry and Biotechnology最新文献_第4页

Integrated Approach for Improving Lutein Production of Chlorella sp. via Adaptive Evolution and Casein Acid Hydrolysate. 通过适应进化和酪蛋白酸水解提高小球藻叶黄素产量的综合方法。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-13 DOI: 10.1007/s12010-025-05279-0

Manlin Yan, Ting Liu, Quanyu Zhao

{"title":"Integrated Approach for Improving Lutein Production of Chlorella sp. via Adaptive Evolution and Casein Acid Hydrolysate.","authors":"Manlin Yan, Ting Liu, Quanyu Zhao","doi":"10.1007/s12010-025-05279-0","DOIUrl":"https://doi.org/10.1007/s12010-025-05279-0","url":null,"abstract":"Lutein is a high value-added product in microalgae. There are several strategies to improve its production, including isolation and selection of high-lutein producing algal strains; strain improvement by metabolic engineering, synthetic biology, or adaptive evolution; and metabolic regulation. In this study, an integrated approach was developed to improve lutein production by adaptive evolution and metabolic regulation. Firstly, a starting strain with a lutein content of ~5.18 mg/g was selected. Secondly, adaptive evolution was performed using an environmental stress of 300 mg/L phenol. The evolved strain, P30, was obtained after 30 cycles; its lutein content had increased to ~5.91 mg/g. Thirdly, the effects of 5-30 g/L NaCl on the lutein contents of the evolved strain were determined. We found that 10 g/L NaCl increased the lutein content by 11% and slightly inhibited growth. No significant changes were observed after adding 20-80 mg/L gibberellin under 10 g/L NaCl conditions. The addition of 10.5 mM casein acid hydrolysate (CAH) promoted the growth of the P30 strain under 10 g/L NaCl conditions. The lutein concentration of Chlorella sp. P30 was 6.91 mg/L at day 5, which was twice the lutein concentration of the starting strain at the same time. When osmotic pressure was removed, the lutein concentration was 8.42 mg/L. The results indicate that CAH supplementation enhances both microalgal growth and lutein biosynthesis. The results of this study provide a valuable reference for the metabolic regulation of lutein biosynthesis.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-10 DOI: 10.1007/s12010-025-05262-9

Bixiong Zhang, Lingli Xu, Yuran Ji, Jiezhuang Li, Liehui Liu, Liangfang Li, Zewei Zhuo, Zhongwen Zheng

{"title":"RHOGTPase-Related Gene Signature Predicts Prognosis, Immunotherapy Response, and Chemotherapy Sensitivity in Colon Cancer.","authors":"Bixiong Zhang, Lingli Xu, Yuran Ji, Jiezhuang Li, Liehui Liu, Liangfang Li, Zewei Zhuo, Zhongwen Zheng","doi":"10.1007/s12010-025-05262-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05262-9","url":null,"abstract":"Rho GTPases are known to promote colon cancer cell invasion and metastasis by modulating cell motility and adhesion. However, the clinical implications of Rho GTPase-related genes in prognosis and treatment response for colon cancer remain underexplored. We identified Rho GTPase-related prognostic genes using univariate Cox regression and applied Least Absolute Shrinkage and Selection Operator (LASSO) regression to refine these genes and develop a prognostic model. The Rho GTPase-related gene signature was analyzed for associations with clinical outcomes, immune status, immunotherapy, and chemotherapy response in colon cancer patients. A Rho GTPase-related 12-gene signature was established to predict prognosis across training and validation cohorts. Both univariate and multivariate analyses confirmed the Rho GTPase risk score as an independent prognostic factor. The model's area under the curve (AUC) and decision curve analysis (DCA) outperformed traditional TNM staging and several existing models. Immune cell analysis showed high Rho GTPase risk scores correlated with increased macrophage/monocyte and cancer-associated fibroblast infiltration. Tumor Immune Dysfunction and Exclusion (TIDE) analysis revealed an association between high Rho GTPase risk scores and immune dysfunction, suggesting potential resistance to immune checkpoint inhibitors in high-risk patients. Additionally, differential sensitivity to 59 chemotherapeutics was observed: high-risk patients showed greater sensitivity to CCT007093, CGP.082996, and AS601245, while low-risk patients were more sensitive to BMS.708163, NSC.87877, and Cisplatin, informing potential treatment choices. The Rho GTPase-related 12-gene signature offers a valuable tool for predicting prognosis and therapeutic response in colon cancer, supporting personalized treatment strategies and improved patient outcomes.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and Catalytic Characterization of β-1,3-Glucan Synthases of Mushroom Ganoderma lucidum. 蘑菇灵芝β-1,3-葡聚糖合成酶的结构与催化表征

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-09 DOI: 10.1007/s12010-025-05252-x

Zi-Ying Zhang, Lei Sun, JianYong Lei, Yu-Meng Yang, Wen-Jing Sun, Xin-Yi Zan, Li-Juan Meng, Feng-Jie Cui

{"title":"Structural and Catalytic Characterization of β-1,3-Glucan Synthases of Mushroom Ganoderma lucidum.","authors":"Zi-Ying Zhang, Lei Sun, JianYong Lei, Yu-Meng Yang, Wen-Jing Sun, Xin-Yi Zan, Li-Juan Meng, Feng-Jie Cui","doi":"10.1007/s12010-025-05252-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05252-x","url":null,"abstract":"β-Glucans are regarded as the key contributors to nutritional and supplementary credits of well-known edible/medicinal mushroom Ganoderma lucidum. However, the biosynthesis pathways of β-glucans and the involved key enzymes/genes in G. lucidum are still not well investigated. The present study cloned and bioinformatically analyzed a 5259-bp gene GLGLS1 and a 5334-bp gene GLGLS2 encoding membrane bound β-1,3-glucan synthases GLGLS1p and GLGLS2p with the predicted Mws of 199.64 kDa and 203.82 kDa from G. lucidum mycelia, respectively. GLGLS2p was further partially purified and identified from cultivated mycelia of G. lucidum as the potential key β-1, 3-glucan synthase for the β-glucan biosynthesis. GLGLS2p showed high specificity to UDP-glucose with a Km value of 79.54 µM and synthesize β-1, 3-glucan with a maximum degree of polymerization (DP) of 54. The AlphaFold 2 and molecular docking indicated that substrate UDP-glucose bound to seven amino acid residues of GLGLS2p via hydrogen bonds. These findings would provide insights into the knowledge of the glucan synthesis and catalytic mechanism of β-1,3-glucan synthases in G. lucidum.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Bacterial Laccase with Improved Catalytic Activity and Thermostability by Rational Design. 通过合理设计提高催化活性和热稳定性的工程细菌漆酶。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-09 DOI: 10.1007/s12010-025-05240-1

Xuting Sun, Xiaofan Lin, Yufan Xian, Faying Zhang, Lingxuan Zhu, Haitao Geng, Wenya Wang, Guimin Zhang

{"title":"Engineering Bacterial Laccase with Improved Catalytic Activity and Thermostability by Rational Design.","authors":"Xuting Sun, Xiaofan Lin, Yufan Xian, Faying Zhang, Lingxuan Zhu, Haitao Geng, Wenya Wang, Guimin Zhang","doi":"10.1007/s12010-025-05240-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05240-1","url":null,"abstract":"Laccases (benzenediol:oxygen oxidoreductases) are important multi-copper oxidases with widespread applications in industry. Here, Bacillus subtilis laccase CotA that has been widely studied was engineered to improve catalytic activity and thermostability via rational design. After iterative mutation of beneficial mutation sites, a triple mutant of CotA laccase (DTA) was obtained, whose catalytic activity and thermostability were improved by 2.7-fold and 1.4-fold compared with the wild-type (WT) CotA, respectively. The enhanced activity of DTA is primarily due to strengthened intermolecular forces in the active site, while its improved thermostability is attributed to increased hydrophobic residues, augmented protein surface flexibility, collectively rendering DTA a more active and stable enzyme with potential industrial applications. Compared to WT, DTA can degrade mycotoxins aflatoxin B1 (55.09% vs 44.51%) and ZEN (zearalenone) (71.59% vs 41.09%) more efficiently, and DTA can also better pretreat lignocellulose, promoting the hydrolysis of cellulose by cellulase. All these indicate that DTA has the potential to be used in industry.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and Risk Assessment of Polycyclic Aromatic Hydrocarbons in the Surface Water of Budhabalanga River Estuary, Chandipur, East Coast of India. 印度东海岸昌迪普尔布哈巴兰加河河口地表水中多环芳烃的赋存及风险评价

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-08 DOI: 10.1007/s12010-025-05248-7

Subhashree Nayak, Smruti Prajna Pradhan, Pratyusha Nayak, Sthitaprajna Nath Sharma, Nishigandha Muduli, Susri Nayak, Lipika Patnaik

引用次数: 0

Effect of Sarpogrelate on Cerebral Venous Sinus Thrombosis Model. 沙棘酸酯对脑静脉窦血栓形成模型的影响。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-07 DOI: 10.1007/s12010-025-05234-z

Xichen Wan, Ye Xiong, Shi Zhou Xing, Xiao Wu, Song Xiao, Peng Liu

{"title":"Effect of Sarpogrelate on Cerebral Venous Sinus Thrombosis Model.","authors":"Xichen Wan, Ye Xiong, Shi Zhou Xing, Xiao Wu, Song Xiao, Peng Liu","doi":"10.1007/s12010-025-05234-z","DOIUrl":"https://doi.org/10.1007/s12010-025-05234-z","url":null,"abstract":"To elucidate the effects of cerebral venous sinus thrombosis (CVST) on mice and the effects of sarpogrelate (S) on CVST, mice were randomly divided into the sham-operated group, CVST group, and CVST + S group (sarpogrelate). Neurological function was evaluated using the rotarod test, balance beam test, and open-field test. Moreover, laser speckle contrast imaging was employed to observe the blood flow in the cerebral cortex of mice, and immunofluorescence was used to quantify the neurons. In the neurofunctional assessment tests (i.e., rotarod test, balance beam test, and open-field test), the CVST group exhibited poorer performance compared to the sham-operated group. However, mice treated with sarpogrelate showed significantly better performance than the CVST group (P < 0.05). Moreover, a significant decrease in cerebral blood flow was observed in the CVST group compared to the sham-operated group (P < 0.01). In contrast, a significant increase in blood flow was observed in the CVST + S group compared to the CVST group (P < 0.05). Furthermore, the number of neurons in the hippocampus and prefrontal cortex of mice in the CVST group was lower than that in the sham group (P < 0.05), and the number of neurons in the hippocampus and prefrontal cortex of the CVST + S group was higher than that of the CVST group (P < 0.05). Sarpogrelate can increase blood flow in the cerebral cortex of mice with CVST, leading to decreased neuronal cell damage and improved neurological function.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Antibacterial, Antioxidant, Cytotoxic Potential of Oscillatoria sp.-Derived Metabolites and Their Molecular Docking with Estrogen Receptor-α. 探讨振荡藻衍生代谢物的抗菌、抗氧化、细胞毒性及其与雌激素受体-α的分子对接。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-06 DOI: 10.1007/s12010-025-05246-9

Santhosh Sigamani, Balasubramani Govindasamy, Balashanmugam Panneerselvam, Jothimani Kannupaiyan, Sivanandam Magudeeswaran, Dhandapani Ramamurthy, Hemalatha Natarajan

{"title":"Exploring Antibacterial, Antioxidant, Cytotoxic Potential of Oscillatoria sp.-Derived Metabolites and Their Molecular Docking with Estrogen Receptor-α.","authors":"Santhosh Sigamani, Balasubramani Govindasamy, Balashanmugam Panneerselvam, Jothimani Kannupaiyan, Sivanandam Magudeeswaran, Dhandapani Ramamurthy, Hemalatha Natarajan","doi":"10.1007/s12010-025-05246-9","DOIUrl":"https://doi.org/10.1007/s12010-025-05246-9","url":null,"abstract":"Anthropogenic activities have led to the accumulation of carcinogenic toxicants in the food chain, posing severe risks to both human and animal health. Bioactive molecules derived from terrestrial and aquatic systems offer promising solutions to various health challenges. Presently, the cyanobacterial metabolites from ethyl acetate extract of Oscillatoria sp. (EAEOs) were evaluated for its antiproliferative, antioxidant, and antimicrobial effects. The EAEOs resulted significant antibacterial zones (12 ± 2.5, 17 ± 1.7, 14 ± 0.3, and 14 ± 2.6 mm) on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Bacillus cereus, respectively. EAEOs also exhibited free radical scavenging activity with IC50 values of 69.68 µg/mL (DPPH) and 144.4 µg/mL (hydroxyl radicals). Followed by, the EAEOs also exhibited significant inhibition of MCF-7 breast cancer cells (IC50: 54.25 µg/mL) compared to HepG2 and A549 cells (72.6 and 85.6 µg/mL), respectively The EAEOs induced apoptosis and chromosomal damage on targeted cancer cells, and their DNA fragmentation were evidenced. Totally, 17 compounds from EAEOs were identified from GC-MS, in which 2-cyclopenten-1-one, 2-hydroxy-3-methyl (23); piperidine, 1, 4-dimethyl (13); 17-pentatriacontene (7.88); and 3-octadecene (7.09%) are major ones. Molecular docking confirmed that acetamide, n-(2-benzoyl-4chlorophenyl)-2-(2-methylpiperidin-1-yl)- (compound-3) showed strong binding affinity (10.13 kcal/mol) with ERα. Overall, the biological potential of Oscillatoria metabolites were found to be significant. Hence, further screening of preponderant compounds and studying the mechanism are warranted to fulfill their applications in food, pharmaceuticals, and other industries.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trigonelline Shields Chondrocytes from Oxidative Damage in Osteoarthritis through Activation of the Keap1/Nrf2/ARE Signaling Pathway. 葫芦巴碱通过激活Keap1/Nrf2/ARE信号通路保护骨关节炎软骨细胞免受氧化损伤。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-05 DOI: 10.1007/s12010-025-05232-1

Chunmei Jiang, Xiaohong He, Aiju Lou, Shiwen He, Qixin Xie, Yuechun Wang, Shan Zhong, Weirong Wu, Qingchun Huang

{"title":"Trigonelline Shields Chondrocytes from Oxidative Damage in Osteoarthritis through Activation of the Keap1/Nrf2/ARE Signaling Pathway.","authors":"Chunmei Jiang, Xiaohong He, Aiju Lou, Shiwen He, Qixin Xie, Yuechun Wang, Shan Zhong, Weirong Wu, Qingchun Huang","doi":"10.1007/s12010-025-05232-1","DOIUrl":"https://doi.org/10.1007/s12010-025-05232-1","url":null,"abstract":"Oxidative stress-induced chondrocyte damage is a key contributor to the progression of osteoarthritis (OA). While trigonelline (TG) possesses anti-inflammatory and antioxidant activities, its functional role and underlying mechanisms in OA remain unclear. In this study, the human chondrocyte cell line CHON-001 was treated with TG alone or in combination with IL-1β or ML385 for 24 h. Chondrocyte injury-related events were assessed using Cell Counting Kit-8 (CCK-8), flow cytometry with Annexin V-FITC/PI kit, Hoechst staining, the probe 2,7-Dichlorofluorescin diacetate (DCFH-DA), SA-β-gal staining, and SOD and MDA assay kits. Our data revealed that TG alleviated IL-1β-induced inflammation, apoptosis, extracellular matrix (ECM) degradation, senescence, and oxidative stress in chondrocytes, accompanied by the downregulation of Keap1 and upregulation of Nrf2, HO-1 and NQO1. ML385 treatment reversed the protective effects of TG against IL-1β-induced injury in chondrocytes. In vivo, the anterior cruciate ligament transection (ACLT) was used to induce a rat OA model, and TG was administered by gavage. OA severity and articular cartilage degradation were evaluated by hematoxylin and eosin (H&E), toluidine blue, Safranin-O staining, and Osteoarthritis Research Society International (OARSI) scoring system. The in vivo data showed that TG attenuated the degeneration and erosion of articular cartilage, suppressed inflammation, and downregulated the levels of Keap1 and iNOS, while upregulating the levels of Nrf2 and Col2a1. In conclusion, our study demonstrated that TG inhibits oxidative stress-induced chondrocyte dysfunction and cartilage degradation by activating the Keap1/Nrf2/ARE signaling pathway.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Role of Microbes in Remediation of High Molecular Weight Polycyclic Aromatic Hydrocarbon Persistence in the Environment. 揭示微生物在环境中高分子量多环芳烃持久性修复中的作用。

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-03 DOI: 10.1007/s12010-025-05245-w

Tony Hadibarata, Kitara Pranareswari Hadibarata

{"title":"Unraveling the Role of Microbes in Remediation of High Molecular Weight Polycyclic Aromatic Hydrocarbon Persistence in the Environment.","authors":"Tony Hadibarata, Kitara Pranareswari Hadibarata","doi":"10.1007/s12010-025-05245-w","DOIUrl":"https://doi.org/10.1007/s12010-025-05245-w","url":null,"abstract":"The persistence and recalcitrance of high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) are potential threats to health and the environment. They result mainly from incomplete combustion of fossil fuels and organic materials, and they tend to accumulate in terrestrial and aquatic ecosystems (particularly in soils, sediments, and water sources). Chronic exposure to HMW PAHs is associated with some of the most dreadful health outcomes, including lung and skin cancers and disorders of the respiratory and immune systems. The study therefore proposes microbial degradation as a promising bioremediation technique for HMW PAHs: pyrene, benzo[a]pyrene, chrysene, and fluoranthene. Aerobic degradations mediated by dioxygenase and dehydrogenase enzymes, as well as anaerobic pathways involving sulfate- and nitrate-reducing bacteria, are discussed. Factors that promote microbial degradation include pH, temperature, nutrient availability, and salinity. While all factors can be biostimulation and bioaugmentation, the study emphasizes these two as effective methods to enhance bioavailability and degradation efficiency. The results provide insightful information for further development of microbial techniques in remediation of HMW PAH-contaminated sites.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaerobic Volatile Fatty Acid Production Performance and Microbial Community Characteristics from Solid Fraction of Alkali-Thermal Treated Waste-Activated Sludge: Focusing on the Effects of Different pH Conditions. 碱热处理废活性污泥固体馏分厌氧挥发性脂肪酸生产性能及微生物群落特征：不同pH条件的影响

IF 3.1 4区生物学

Applied Biochemistry and Biotechnology Pub Date : 2025-05-03 DOI: 10.1007/s12010-025-05244-x

Xiu-Fang Li, Wen-Shuai Zhang, Sheng Qi, Jun-Feng Zhao, Zhao-Yong Sun, Yue-Qin Tang

{"title":"Anaerobic Volatile Fatty Acid Production Performance and Microbial Community Characteristics from Solid Fraction of Alkali-Thermal Treated Waste-Activated Sludge: Focusing on the Effects of Different pH Conditions.","authors":"Xiu-Fang Li, Wen-Shuai Zhang, Sheng Qi, Jun-Feng Zhao, Zhao-Yong Sun, Yue-Qin Tang","doi":"10.1007/s12010-025-05244-x","DOIUrl":"https://doi.org/10.1007/s12010-025-05244-x","url":null,"abstract":"The waste-activated sludge (WAS) is rich in organic matter and various nutrients. Alkali-thermal hydrolysis of WAS can be employed to produce a liquid fertilizer with high plant-promoting nutrient content. However, the solid fraction (abbreviated as SF) generated from this process requires further treatment. Although there have been studies on the recovery of plant nutrients from WAS via alkali-thermal hydrolysis, researches on the safe treatment of the SF are limited. This study aims to explore the potential and the microbiological mechanisms on anaerobic volatile fatty acid (VFA) production from the SF under different pH conditions (i.e., 6, 7, 8, 9, and 10). The results showed that the VFA yield was highest at pH 6, reaching 4095.84 mg COD/L (i.e., 0.16 g-COD/g-volatile solids), followed by pH 10, 8, 7, and 9, with acetate being the main component (> 56%). Microbial community analysis revealed that members in phyla Firmicutes and Bacteroidota constituted the main acid-producing microbial community during the anaerobic fermentation of SF. Furthermore, different pH conditions influenced the yield and composition of VFAs by altering the structure and functions of microbial community. This research provides a new direction for the fully resourceful utilization of sludge by producing both liquid fertilizer and VFAs from WAS.","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0