Cell stem cell最新文献_第4页

Transgene-free generation of mouse post-gastrulation whole embryo models solely from naive ESCs and iPSCs 用未成熟的ESCs和iPSCs制备小鼠原肠胚后全胚胎模型

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-08-07 DOI: 10.1016/j.stem.2025.07.005

Alperen Yilmaz, Gulben Gurhan, Mehmet-Yunus Comar, Sergey Viukov, Inbal Serfaty, Mert Gayretli, Sergey Golenchenko, Dmitry Lokshtanov, Shahd Ashouokhi, Angel Polanco, Idan Berlad, Tae-Won Ha, Alejandro Aguilera-Castrejon, Shadi Tarazi, Marina Cohen, Nir Livnat, Komal Kumar, Hisham Cholakkal, Nathan Levy, Nir Yosef, Jacob H. Hanna

{"title":"Transgene-free generation of mouse post-gastrulation whole embryo models solely from naive ESCs and iPSCs","authors":"Alperen Yilmaz, Gulben Gurhan, Mehmet-Yunus Comar, Sergey Viukov, Inbal Serfaty, Mert Gayretli, Sergey Golenchenko, Dmitry Lokshtanov, Shahd Ashouokhi, Angel Polanco, Idan Berlad, Tae-Won Ha, Alejandro Aguilera-Castrejon, Shadi Tarazi, Marina Cohen, Nir Livnat, Komal Kumar, Hisham Cholakkal, Nathan Levy, Nir Yosef, Jacob H. Hanna","doi":"10.1016/j.stem.2025.07.005","DOIUrl":"https://doi.org/10.1016/j.stem.2025.07.005","url":null,"abstract":"The generation of post-gastrulation stem cell-derived mouse embryo models (SEMs) exclusively from naive embryonic stem cells (nESCs) has underscored their ability to give rise to embryonic and extra-embryonic lineages. However, existing protocols for mouse SEMs rely on the separate induction of extra-embryonic lineages and on ectopic expression of transcription factors to induce nESC differentiation into trophectoderm (TE) or primitive endoderm (PrE). Here, we demonstrate that mouse nESCs and naive induced pluripotent stem cells (niPSCs) can be simultaneously co-induced, via signaling pathway modulation, to generate PrE and TE extra-embryonic cells that self-organize into embryonic day (E) 8.5–E8.75 transgene-free (TF) SEMs. We also devised an alternative condition (AC) naive media that in vitro stabilizes TF-SEM-competent OCT4+/NANOG+ nESC colonies that co-express antagonistic CDX2 and/or GATA6 extra-embryonic fate master regulators and self-renew while remaining poised for TE and PrE differentiation, respectively. These findings improve mouse SEM strategies and shed light on amplifying an inherent and dormant extra-embryonic plasticity of mouse naive pluripotent cells in vitro.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"9 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144792325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plot twist: TET2 clones save the brain 剧情转折：TET2克隆人拯救了大脑

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-08-07 DOI: 10.1016/j.stem.2025.07.001

Maria A. Telpoukhovskaia, Jennifer J. Trowbridge

引用次数: 0

Vascular organoids get a speed boost for regenerative repair 类血管器官的再生修复速度加快

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-08-07 DOI: 10.1016/j.stem.2025.07.002

Danielle Klinger, Jeffrey A. Naftaly, Kristy Red-Horse

引用次数: 0

Revolutionizing islet transplantation with a preconditioning boost for beta cell survival 革命性的胰岛移植与预处理促进β细胞存活

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-08-07 DOI: 10.1016/j.stem.2025.06.011

Kewen Hu, Yajie Chen, Zhen Zhang

引用次数: 0

TET2-mutant myeloid cells mitigate Alzheimer’s disease progression via CNS infiltration and enhanced phagocytosis in mice tet2突变骨髓细胞通过中枢神经系统浸润和增强吞噬作用减轻小鼠阿尔茨海默病的进展

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-08-01 DOI: 10.1016/j.stem.2025.07.011

Katie A. Matatall, Trisha K. Wathan, Minh Nguyen, Hu Chen, Alexandra McDonald, Guantong Qi, Julia A. Belk, Marcus A. Florez, Duy T. Le, Temitope Olarinde, Caitlyn Vlasschaert, Marco M. Buttigieg, Chih-wei Fan, Saul Carcamo, Ruoqiong Cao, Daniel E. Kennedy, Arushana A. Maknojia, Apoorva Thatavarty, Josaura V. Fernandez Sanchez, Hind Bouzid, Katherine Y. King

引用次数: 0

Comparative single-cell lineage tracing identifies distinct adipocyte precursor dynamics in skin and inguinal fat 比较单细胞谱系追踪识别皮肤和腹股沟脂肪中不同的脂肪细胞前体动力学

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-07-30 DOI: 10.1016/j.stem.2025.07.004

Guillermo C. Rivera-Gonzalez, Emily G. Butka, Carolynn E. Gonzalez, Rachel L. Mintz, Sarah S. Kleb, Violet Josephson, Wenjun Kong, Kunal Jindal, Kenji Kamimoto, Brett A. Shook, Matthew S. Rodeheffer, Samantha A. Morris

{"title":"Comparative single-cell lineage tracing identifies distinct adipocyte precursor dynamics in skin and inguinal fat","authors":"Guillermo C. Rivera-Gonzalez, Emily G. Butka, Carolynn E. Gonzalez, Rachel L. Mintz, Sarah S. Kleb, Violet Josephson, Wenjun Kong, Kunal Jindal, Kenji Kamimoto, Brett A. Shook, Matthew S. Rodeheffer, Samantha A. Morris","doi":"10.1016/j.stem.2025.07.004","DOIUrl":"https://doi.org/10.1016/j.stem.2025.07.004","url":null,"abstract":"White adipose tissue supports essential physiological functions through adipocyte precursor cells (APCs), comprising progenitors and preadipocytes, which generate mature adipocytes during depot expansion. Using single-cell RNA sequencing-based lineage tracing, we characterize APCs in skin adipose tissue—a depot uniquely capable of rapid adipogenesis compared with other sites, such as inguinal adipose. We identify a previously uncharacterized population of immature preadipocytes and reveal distinct differentiation potentials among APCs. Contrary to traditional stepwise differentiation models, progenitors predominantly generate committed preadipocytes, whereas preexisting preadipocytes accumulate in immature states with divergent potential. Leveraging this refined APC hierarchy, we uncover Sox9 as a crucial regulator of progenitor proliferation and adipogenic differentiation. Cross-depot transplantation further demonstrates how intrinsic and extrinsic factors differentially regulate skin progenitor behavior, highlighting distinct adipogenic dynamics between skin and inguinal depots. Together, these insights redefine the cellular hierarchy and molecular mechanisms underpinning rapid adipogenesis in skin adipose tissue.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"27 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical reprogramming of human blood cells to pluripotent stem cells 人类血液细胞化学重编程为多能干细胞

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-07-30 DOI: 10.1016/j.stem.2025.07.003

Fangqi Peng, Yanglu Wang, Lin Cheng, Ruyi Cai, Xiaodi Fu, Zhihan Yang, Ruoqi Cheng, Weizhen Zeng, Yingshuai Dong, Jingxiao Cao, Jingping Mao, Jingran Zeng, Tianxing Liu, Guanxian Chen, Qi Lei, Lipeng Wang, Lulu Liu, Shicheng Sun, Cheng Li, Rong Mu, Hongkui Deng

{"title":"Chemical reprogramming of human blood cells to pluripotent stem cells","authors":"Fangqi Peng, Yanglu Wang, Lin Cheng, Ruyi Cai, Xiaodi Fu, Zhihan Yang, Ruoqi Cheng, Weizhen Zeng, Yingshuai Dong, Jingxiao Cao, Jingping Mao, Jingran Zeng, Tianxing Liu, Guanxian Chen, Qi Lei, Lipeng Wang, Lulu Liu, Shicheng Sun, Cheng Li, Rong Mu, Hongkui Deng","doi":"10.1016/j.stem.2025.07.003","DOIUrl":"https://doi.org/10.1016/j.stem.2025.07.003","url":null,"abstract":"Chemical reprogramming offers a fundamentally innovative approach for generating human chemically induced pluripotent stem (hCiPS) cells using small molecules. Our recent studies showed that this approach was highly efficient in reprogramming human fibroblasts to hCiPS cells. However, generating hCiPS cells from human blood cells, which are the most accessible and convenient source for reprogramming, remains a challenge. In this study, we established a robust method that successfully generated hCiPS cells from both cord blood and adult peripheral blood cells. This method achieved efficient reprogramming with both fresh and cryopreserved blood cells across different donors. Notably, this method also efficiently generated an average of over 100 hCiPS colonies from just a single drop of fingerstick blood. These results highlight the advantages of chemical reprogramming for generating hCiPS cells from a blood source and represent a next-generation platform for efficient, scalable, and convenient stem cell production with broad applications in regenerative medicine.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"300 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-canonical functions of DNMT3A in hematopoietic stem cells regulate telomerase activity and genome integrity DNMT3A在造血干细胞中的非规范功能调节端粒酶活性和基因组完整性

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-07-17 DOI: 10.1016/j.stem.2025.06.010

Infencia Xavier Raj, Won Kyun Koh, Jessica Harrison, Christine R. Zhang, Barbara Soares, Roberta Amato, Aishwarya Krishnan, David R. O’Leary, Hassan Bjeije, Tyler M. Parsons, Wentao Han, Andrew L. Young, Ting Wang, Luis F.Z. Batista, Grant A. Challen

{"title":"Non-canonical functions of DNMT3A in hematopoietic stem cells regulate telomerase activity and genome integrity","authors":"Infencia Xavier Raj, Won Kyun Koh, Jessica Harrison, Christine R. Zhang, Barbara Soares, Roberta Amato, Aishwarya Krishnan, David R. O’Leary, Hassan Bjeije, Tyler M. Parsons, Wentao Han, Andrew L. Young, Ting Wang, Luis F.Z. Batista, Grant A. Challen","doi":"10.1016/j.stem.2025.06.010","DOIUrl":"https://doi.org/10.1016/j.stem.2025.06.010","url":null,"abstract":"DNMT3A is a critical regulator of hematopoietic stem cell (HSC) fate decisions and the most recurrently mutated gene in human clonal hematopoiesis (CH). DNMT3A is described as a DNA methyltransferase enzyme, but cells with DNMT3A loss of function show minor changes in DNA methylation that do not correlate with altered gene expression. To explore the possibility that Dnmt3a has DNA-methylation-independent functions in HSCs, we created an allelic series of mice with varying levels of DNA-methylation-impaired Dnmt3a. Clonal expansion of Dnmt3a-deficient HSCs was rescued by Dnmt3a proteins lacking DNA methylation capacity, suggesting that Dnmt3a has important non-canonical functions in HSCs. Dnmt3a-null HSCs can be transplanted indefinitely, implying the ability to circumvent mechanisms that limit the replicative lifespan of HSCs, such as telomere shortening. Dnmt3a-null HSCs show increased telomerase activity and sustain telomere length over serial transplantation, revealing a previously unidentified role for DNMT3A mutations in regulating HSC longevity that is unrelated to DNA methylation function.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"5 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial ADP-heptose triggers stem cell regeneration in the intestinal epithelium following injury 细菌adp -庚糖触发肠上皮损伤后干细胞再生

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-07-11 DOI: 10.1016/j.stem.2025.06.009

Shawn Goyal, Cynthia X. Guo, Ojas Singh, Adrienne Ranger, Caitlin F. Harrigan, Justin Meade, Alexander Luchak, Derek K. Tsang, Herbert Y. Gaisano, Nan Gao, Scott A. Yuzwa, Jeffrey L. Wrana, Dana J. Philpott, Scott D. Gray-Owen, Stephen E. Girardin

{"title":"Bacterial ADP-heptose triggers stem cell regeneration in the intestinal epithelium following injury","authors":"Shawn Goyal, Cynthia X. Guo, Ojas Singh, Adrienne Ranger, Caitlin F. Harrigan, Justin Meade, Alexander Luchak, Derek K. Tsang, Herbert Y. Gaisano, Nan Gao, Scott A. Yuzwa, Jeffrey L. Wrana, Dana J. Philpott, Scott D. Gray-Owen, Stephen E. Girardin","doi":"10.1016/j.stem.2025.06.009","DOIUrl":"https://doi.org/10.1016/j.stem.2025.06.009","url":null,"abstract":"ADP-heptose (ADP-Hep), a metabolite produced by gram-negative bacteria, is detected in the host cytosol by the kinase ALPK1, which engages TIFA-dependent innate immune responses. However, the function of ALPK1-TIFA signaling in primary cells and in physiological settings remains poorly understood. Here, we showed that, in the intestinal epithelium, ALPK1 and TIFA were mainly expressed by the intestinal stem cell (ISC) pool, where they controlled the replacement of homeostatic ISCs by new revival stem cells (revSCs) following injury. Mechanistically, ADP-Hep triggered pro-inflammatory nuclear factor κB (NF-κB) signaling and tumor necrosis factor (TNF)-dependent ISC apoptosis, which initiated a transforming growth factor β (TGF-β)- and YAP-dependent revSC program. Single-cell transcriptomics and lineage-tracing experiments identified Paneth cells as a cell of origin for revSC induction in response to ADP-Hep. In vivo, revSC emergence following irradiation or dextran-sodium-sulfate-induced injury was blunted in Tifa−/− mice. Together, our work reveals that ALPK1-TIFA signaling contributes to ISC turnover in response to bacterial detection in the intestine.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"21 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From symbiote to bad neighbor: The intestinal microbiome as a driver of CHIP 从共生体到坏邻居：肠道微生物组作为CHIP的驱动因素

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-07-03 DOI: 10.1016/j.stem.2025.06.003

Courtney M. Cowan, Eric M. Pietras

引用次数: 0