Cell stem cell最新文献

Cognitive impairment in epilepsy progression: Adult neurogenesis loss at critical window

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-02-06 DOI: 10.1016/j.stem.2025.01.004

Yi Wang, Zhong Chen

引用次数: 0

Human iPSCs offer an alternative for modeling vascular malformation

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-02-06 DOI: 10.1016/j.stem.2025.01.008

Stefanie DeFronzo, Guohao Dai

引用次数: 0

Neural stem cell quiescence and activation dynamics are regulated by feedback input from their progeny under homeostatic and regenerative conditions

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-02-06 DOI: 10.1016/j.stem.2025.01.001

Alina Marymonchyk, Raquel Rodriguez-Aller, Ashleigh Willis, Frédéric Beaupré, Sareen Warsi, Marina Snapyan, Valérie Clavet-Fournier, Flavie Lavoie-Cardinal, David R. Kaplan, Freda D. Miller, Armen Saghatelyan

{"title":"Neural stem cell quiescence and activation dynamics are regulated by feedback input from their progeny under homeostatic and regenerative conditions","authors":"Alina Marymonchyk, Raquel Rodriguez-Aller, Ashleigh Willis, Frédéric Beaupré, Sareen Warsi, Marina Snapyan, Valérie Clavet-Fournier, Flavie Lavoie-Cardinal, David R. Kaplan, Freda D. Miller, Armen Saghatelyan","doi":"10.1016/j.stem.2025.01.001","DOIUrl":"https://doi.org/10.1016/j.stem.2025.01.001","url":null,"abstract":"Life-long maintenance of stem cells implies that feedback mechanisms from the niche regulate their quiescence/activation dynamics. Here, in the mouse adult subventricular neural stem cell (NSC) niche, we charted a precise spatiotemporal map of functional responses in NSCs induced by multiple niche cells and used machine learning to predict NSC interactions with specific niche cell types. We revealed a feedback mechanism whereby the NSC proliferative state is directly repressed by transient amplifying cells (TAPs), their rapidly dividing progeny. NSC processes wrap around TAPs and display hotspots of Ca2+ activity at their points of contact, mediated by ephrin (Efn) signaling. The modulation of Efn signaling or TAP ablation altered the Ca2+ signature of NSCs, leading to their activation. In vivo optogenetic modulation of Ca2+ dynamics abrogated NSC activation and prevented niche replenishment. Thus, TAP-to-NSC feedback signaling controls stem cell quiescence and activation, providing a mechanism to maintain stem cell pools throughout life.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"42 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-birth stem cell education: A gift from mother’s bugs

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-02-06 DOI: 10.1016/j.stem.2025.01.009

Chenyan Huang, Ai Ing Lim

引用次数: 0

Two is better than one: Advancing gene therapy protocols for enhanced safety and efficacy

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-02-06 DOI: 10.1016/j.stem.2024.12.009

Giulia Hardouin, Annarita Miccio

引用次数: 0

Molecular signature of primate astrocytes reveals pathways and regulatory changes contributing to human brain evolution

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-02-04 DOI: 10.1016/j.stem.2024.12.011

Katarzyna Ciuba, Aleksandra Piotrowska, Debadeep Chaudhury, Bondita Dehingia, Eryk Duński, Rüdiger Behr, Karolina Soroczyńska, Małgorzata Czystowska-Kuźmicz, Misbah Abbas, Edyta Bulanda, Sylwia Gawlik-Zawiślak, Sylwia Pietrzak, Izabela Figiel, Jakub Włodarczyk, Alexei Verkhratsky, Marcin Niedbała, Wojciech Kaspera, Tomasz Wypych, Bartosz Wilczyński, Aleksandra Pękowska

{"title":"Molecular signature of primate astrocytes reveals pathways and regulatory changes contributing to human brain evolution","authors":"Katarzyna Ciuba, Aleksandra Piotrowska, Debadeep Chaudhury, Bondita Dehingia, Eryk Duński, Rüdiger Behr, Karolina Soroczyńska, Małgorzata Czystowska-Kuźmicz, Misbah Abbas, Edyta Bulanda, Sylwia Gawlik-Zawiślak, Sylwia Pietrzak, Izabela Figiel, Jakub Włodarczyk, Alexei Verkhratsky, Marcin Niedbała, Wojciech Kaspera, Tomasz Wypych, Bartosz Wilczyński, Aleksandra Pękowska","doi":"10.1016/j.stem.2024.12.011","DOIUrl":"https://doi.org/10.1016/j.stem.2024.12.011","url":null,"abstract":"Astrocytes contribute to the development and regulation of the higher-level functions of the brain, the critical targets of evolution. However, how astrocytes evolve in primates is unsettled. Here, we obtain human, chimpanzee, and macaque induced pluripotent stem-cell-derived astrocytes (iAstrocytes). Human iAstrocytes are bigger and more complex than the non-human primate iAstrocytes. We identify new loci contributing to the increased human astrocyte. We show that genes and pathways implicated in long-range intercellular signaling are activated in the human iAstrocytes and partake in controlling iAstrocyte complexity. Genes downregulated in human iAstrocytes frequently relate to neurological disorders and were decreased in adult brain samples. Through regulome analysis and machine learning, we uncover that functional activation of enhancers coincides with a previously unappreciated, pervasive gain of “stripe” transcription factor binding sites. Altogether, we reveal the transcriptomic signature of primate astrocyte evolution and a mechanism driving the acquisition of the regulatory potential of enhancers.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"84 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adult bi-paternal offspring generated through direct modification of imprinted genes in mammals

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-01-28 DOI: 10.1016/j.stem.2025.01.005

Zhi-kun Li, Li-bin Wang, Le-yun Wang, Xue-han Sun, Ze-hui Ren, Si-nan Ma, Yu-long Zhao, Chao Liu, Gui-hai Feng, Tao Liu, Tian-shi Pan, Qing-tong Shan, Kai Xu, Guan-zheng Luo, Qi Zhou, Wei Li

{"title":"Adult bi-paternal offspring generated through direct modification of imprinted genes in mammals","authors":"Zhi-kun Li, Li-bin Wang, Le-yun Wang, Xue-han Sun, Ze-hui Ren, Si-nan Ma, Yu-long Zhao, Chao Liu, Gui-hai Feng, Tao Liu, Tian-shi Pan, Qing-tong Shan, Kai Xu, Guan-zheng Luo, Qi Zhou, Wei Li","doi":"10.1016/j.stem.2025.01.005","DOIUrl":"https://doi.org/10.1016/j.stem.2025.01.005","url":null,"abstract":"Imprinting abnormalities pose a significant challenge in applications involving embryonic stem cells, induced pluripotent stem cells, and animal cloning, with no universal correction method owing to their complexity and stochastic nature. In this study, we targeted these defects at their source—embryos from same-sex parents—aiming to establish a stable, maintainable imprinting pattern de novo in mammalian cells. Using bi-paternal mouse embryos, which exhibit severe imprinting defects and are typically non-viable, we introduced frameshift mutations, gene deletions, and regulatory edits at 20 key imprinted loci, ultimately achieving the development of fully adult animals, albeit with a relatively low survival rate. The findings provide strong evidence that imprinting abnormalities are a primary barrier to unisexual reproduction in mammals. Moreover, this approach can significantly improve developmental outcomes for embryonic stem cells and cloned animals, opening promising avenues for advancements in regenerative medicine.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"39 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide CRISPR activation screening in senescent cells reveals SOX5 as a driver and therapeutic target of rejuvenation

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-01-28 DOI: 10.1016/j.stem.2025.01.007

Yaobin Jing, Xiaoyu Jiang, Qianzhao Ji, Zeming Wu, Wei Wang, Zunpeng Liu, Pedro Guillen-Garcia, Concepcion Rodriguez Esteban, Pradeep Reddy, Steve Horvath, Jingyi Li, Lingling Geng, Qinchao Hu, Si Wang, Juan Carlos Izpisua Belmonte, Jie Ren, Weiqi Zhang, Jing Qu, Guang-Hui Liu

引用次数: 0

Pluripotent stem-cell-derived therapies in clinical trial: A 2025 update

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-01-25 DOI: 10.1016/j.stem.2025.01.003

Agnete Kirkeby, Heather Main, Melissa Carpenter

引用次数: 0

Mouse totipotent blastomere-like cells model embryogenesis from zygotic genome activation to post implantation 小鼠全能卵裂球样细胞从合子基因组激活到着床后的胚胎发生模型

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-01-17 DOI: 10.1016/j.stem.2024.12.006

Bing Peng, Qingyi Wang, Feixiang Zhang, Hui Shen, Peng Du

{"title":"Mouse totipotent blastomere-like cells model embryogenesis from zygotic genome activation to post implantation","authors":"Bing Peng, Qingyi Wang, Feixiang Zhang, Hui Shen, Peng Du","doi":"10.1016/j.stem.2024.12.006","DOIUrl":"https://doi.org/10.1016/j.stem.2024.12.006","url":null,"abstract":"Embryo development begins with zygotic genome activation (ZGA), eventually generating blastocysts for implantation. However, in vitro systems modeling the pre-implantation development are still absent and challenging. Here, we used mouse totipotent blastomere-like cells (TBLCs) to develop spontaneous differentiation and blastoid formation systems, respectively. We found Wnt signaling enabled the rapid expansion of TBLCs and the optimization of their culture medium. We successfully developed a TBLC-spontaneous differentiation system in which mouse TBLCs (mTBLCs) firstly converted into two types of ZGA-like cells (ZLCs) distinguished by Zscan4 expression. Surprisingly, Zscan4-, but not Zscan4+, ZLCs further passed through intermediate 4-cell and then 8-cell/morula stages to produce epiblast, primitive endoderm, and trophectoderm lineages. Significantly, single TBLCs underwent expansion, compaction, and polarization to efficiently generate blastocyst-like structures and even post-implantation egg-cylinder-like structures. Conclusively, we established TBLC-based differentiation and embryo-like structure formation systems to model early embryonic development, offering criteria for evaluating and understanding totipotency.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"27 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142987693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0