Chemical reprogramming of human blood cells to pluripotent stem cells

IF 20.4 1区医学 Q1 CELL & TISSUE ENGINEERING

Cell stem cell Pub Date : 2025-07-30 DOI:10.1016/j.stem.2025.07.003

Fangqi Peng, Yanglu Wang, Lin Cheng, Ruyi Cai, Xiaodi Fu, Zhihan Yang, Ruoqi Cheng, Weizhen Zeng, Yingshuai Dong, Jingxiao Cao, Jingping Mao, Jingran Zeng, Tianxing Liu, Guanxian Chen, Qi Lei, Lipeng Wang, Lulu Liu, Shicheng Sun, Cheng Li, Rong Mu, Hongkui Deng

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引用次数: 0

Abstract

Chemical reprogramming offers a fundamentally innovative approach for generating human chemically induced pluripotent stem (hCiPS) cells using small molecules. Our recent studies showed that this approach was highly efficient in reprogramming human fibroblasts to hCiPS cells. However, generating hCiPS cells from human blood cells, which are the most accessible and convenient source for reprogramming, remains a challenge. In this study, we established a robust method that successfully generated hCiPS cells from both cord blood and adult peripheral blood cells. This method achieved efficient reprogramming with both fresh and cryopreserved blood cells across different donors. Notably, this method also efficiently generated an average of over 100 hCiPS colonies from just a single drop of fingerstick blood. These results highlight the advantages of chemical reprogramming for generating hCiPS cells from a blood source and represent a next-generation platform for efficient, scalable, and convenient stem cell production with broad applications in regenerative medicine.

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人类血液细胞化学重编程为多能干细胞

化学重编程为利用小分子产生人类化学诱导多能干细胞（hCiPS）提供了一种根本性的创新方法。我们最近的研究表明，这种方法在将人成纤维细胞重编程为hCiPS细胞方面非常有效。然而，从人类血细胞中生成hCiPS细胞仍然是一个挑战，因为它是最容易获得和最方便的重编程来源。在这项研究中，我们建立了一种强大的方法，成功地从脐带血和成人外周血中生成hCiPS细胞。该方法对不同供体的新鲜和低温保存的血细胞进行了高效的重编程。值得注意的是，这种方法还能从一滴血中高效地产生平均超过100个hCiPS菌落。这些结果突出了化学重编程从血液来源生成hCiPS细胞的优势，代表了下一代高效、可扩展和方便的干细胞生产平台，在再生医学中具有广泛的应用。

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来源期刊

Cell stem cell 生物-细胞生物学

CiteScore

37.10

自引率

2.50%

发文量

151

审稿时长

42 days

期刊介绍： Cell Stem Cell is a comprehensive journal covering the entire spectrum of stem cell biology. It encompasses various topics, including embryonic stem cells, pluripotency, germline stem cells, tissue-specific stem cells, differentiation, epigenetics, genomics, cancer stem cells, stem cell niches, disease models, nuclear transfer technology, bioengineering, drug discovery, in vivo imaging, therapeutic applications, regenerative medicine, clinical insights, research policies, ethical considerations, and technical innovations. The journal welcomes studies from any model system providing insights into stem cell biology, with a focus on human stem cells. It publishes research reports of significant importance, along with review and analysis articles covering diverse aspects of stem cell research.