Advanced biomedical research最新文献

{"title":"Protective Effect of Whey Protein Supplement Against Rotenone Induced Motor Dysfunction in a Rat Model of Parkinson Disease.","authors":"Parvin Zarei, Behnam Amirpour-Najafabadi, Parnian Sam-Sani, Mohammad Hassan Sakhaie, Mehdi Sadegh","doi":"10.4103/abr.abr_178_23","DOIUrl":"10.4103/abr.abr_178_23","url":null,"abstract":"<p><strong>Background: </strong>We aimed to investigate the effects of whey protein (WP) supplements in a rat model of rotenone-induced locomotor and biochemical features of Parkinson's disease (PD).</p><p><strong>Materials and methods: </strong>Male Wistar rats were used. Daily injections of rotenone (2 mg/kg; i.p.) for 16 days were used to induce PD. WP or soy protein (SP) at 1, 2, and 4 g/rats were administrated daily by gavage. Motor skills were measured in rats 24 h after the last injection using the bar test, grid test, rearing, and open field tests. In the following, striatum tissue was isolated for biochemical measurements. ELISA kits were used for biochemical assessments.</p><p><strong>Results: </strong>While rotenone caused a significant increase in the delay time in both the bar and grid tests and a significant decrease in the motor activities were observed in both rearing and spontaneous movement tests in the rotenone group, supplementation with 2 and 4 g of WP, but not SP, significantly decreased the delay time in the bar and grid tests and also significantly increased both rearing and spontaneous movements. Additionally, rotenone caused a significant decrease in striatal levels of dopamine and glutathione and significantly increased apoptotic caspases 8, 9, and Cytochrome C, while 2 and 4 g of WP, but not SP, significantly reversed these effects.</p><p><strong>Conclusions: </strong>WP appears to have neuroprotective effects against rotenone-induced neurotoxicity and motor dysfunction, so it may be effective in the control of PD.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"93"},"PeriodicalIF":0.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Deep Local Lung Hyperthermia on COVID-19 Cancer Patients.","authors":"Mohammadbagher Tavakoli, Reza Moghareabed, Hossein Taheri, Mahta Noorbakhsh Dehkordy, Elaheh Nasri, Mohsen Saeb, Simin Hemati","doi":"10.4103/abr.abr_75_23","DOIUrl":"10.4103/abr.abr_75_23","url":null,"abstract":"<p><strong>Background: </strong>The goal of this study was to assess the impact of deep local hyperthermia on oxygen (O₂) saturation and infected volumes of lungs on coronavirus disease 2019 (COVID-19) cancer patients.</p><p><strong>Materials and methods: </strong>Fifty patients who suffered from COVID-19 (according to their computed tomography (CT) images and laboratory findings) were included in this study. The mentioned patients were divided into two groups (I and II) with thirty-five participants. The infected volumes and COVID-19 infectious locations were diagnosed using their CT images, and deep local hyperthermia was performed for group II. After three consequent days, the SPO_2, D-dimer, and infected volumes of lung parenchyma of both groups were compared to each other.</p><p><strong>Results: </strong>For group II, the mean ± SD (standard deviation) of O₂ pressure saturation (SPO₂) before/after hyperthermia was 85 ± 0.0/91.3 ± 0.5, respectively, while for group I, the mean ± SD of SPO₂ before/after 3 days was 85 ± 0.0/88 ± 0.2, respectively. For infected volumes of lungs before/after hyperthermia in group II, the mean ± SD was 31.36 ± 3.13/4 ± 1.53, respectively. Nonetheless, the infected volumes of lungs for group I were 34.21 ± 3.41/10 ± 2.12 before/after three days. For group II, the amount of D-dimer before/after hyperthermia was 3200 ± 106/510 ± 121, respectively. However, for group I, it was 3100/740 before/after the consequent three days, respectively.</p><p><strong>Conclusion: </strong>Deep local lung hyperthermia for COVID-19 cancer patients is suggested, as a result of its positive impacts on SPO₂ improvement and also D-dimer serum level, C-reactive protein, and Lactate dehydrogenaze reduction for the mentioned patients.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"92"},"PeriodicalIF":0.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Prevalence of Antimicrobial Resistance Genes in Multidrug-Resistant Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Clinical Isolates after COVID-19 Pandemic, North Iran.","authors":"Golnar Rahimzadeh, Shaghayegh Rezai, Reza Valadan, Raha Rezai, Saman Soleimanpour, Laleh Vahedi, Somayeh Sheidaei, Masoud Moradi, Mohammad Sadegh Rezai, Ebrahim Nemati","doi":"10.4103/abr.abr_341_24","DOIUrl":"10.4103/abr.abr_341_24","url":null,"abstract":"<p><strong>Background: </strong>Amid the COVID-19 pandemic, the surge in hospital admissions and widespread use of broad-spectrum antibiotics have heightened the risk of hospital-acquired infections from multidrug-resistant (MDR) organisms, particularly <i>Escherichia coli</i>. It is imperative to implement stringent measures to curb the spread of antimicrobial resistance in hospitals and devise robust treatment strategies for patients grappling with such infections. To confront this challenge, a comprehensive study was undertaken to examine MDR extended-spectrum beta-lactamase (MDR-ESBL)-producing <i>Escherichia coli</i> isolates from patients with nosocomial infections following the COVID-19 pandemic in Northern Iran.</p><p><strong>Materials and methods: </strong>The current study was conducted as a cross-sectional study. A total of 12,834 samples were collected from patients with healthcare-associated infections at four designated corona centers in Northern Iran, following the COVID-19 pandemic. Antimicrobial resistance was determined using standard broth micro-dilution, while resistance genes were accurately detected using the multiplex PCR method.</p><p><strong>Results: </strong>The results indicated that meropenem and ciprofloxacin had a resistance rate of 100% and 98.2%, respectively, while piperacillin-tazobactam showed the highest sensitivity rate at 54.4%. The frequency of specific genes, including <i>bla</i> _IMP <i>, bla</i> _TEM <i>, AcrA, AcrB, bla</i> _CTX <i>, bla</i> _OXA-58 <i>, aaclb, bla</i> _SHV, and <i>aacla</i>, were found to be 100%, 100%, 99.1%, 99.1%, 91.2%, 80.7%, 64.9%, 44.7%, and 37.7%, respectively.</p><p><strong>Conclusions: </strong>In the current study, over 50% of MDR-ESBL-producing <i>Escherichia coli</i> isolates exhibited resistance to antibiotics. A combination of antibiotics, including piperacillin-tazobactam and colistin, is recommended for treating extensively drug-resistant <i>Escherichia coli</i> infections.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"105"},"PeriodicalIF":0.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Detection of Diabetic Nephropathy Based on Urinary and Serum Biomarkers: An Updated Systematic Review.","authors":"Farzaneh Karimi, Mostafa Moazamfard, Rasul Taghvaeefar, Shahla Sohrabipour, Aghdas Dehghani, Reza Azizi, Negar Dinarvand","doi":"10.4103/abr.abr_461_23","DOIUrl":"10.4103/abr.abr_461_23","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) is a leading cause of chronic kidney disease (CKD) and end-stage renal disease worldwide, particularly among individuals with type 2 diabetes mellitus (T2DM). Early detection and intervention are crucial in slowing the progression of DN and improving patient outcomes. Traditional diagnostic methods, such as the measurement of albuminuria and serum creatinine, often fail to detect early renal damage because structural kidney damage may occur before albumin excretion. This systematic review aims to evaluate the diagnostic value of various urinary and serum biomarkers in the early detection of DN in patients with T2DM. A comprehensive literature search was conducted using databases such as PubMed, Scopus, and Web of Science. We only considered studies involving human populations for inclusion in our analysis. Animal and <i>in vitro</i> studies were excluded from our review. Our analysis of 17 observational studies identified several key serum biomarkers, such as netrin-1, osteopontin, adiponectin, and specific cytokines (e.g., IL-6, IL-8), which show significant promise for early detection of DN. Urinary biomarkers, including neutrophil gelatinase-associated lipocalin (NGAL), transferrin, N-acetyl-β-D-glucosaminidase (NAG), and various cytokines, have also proven to be reliable indicators. The combination of both serum and urinary biomarkers may enhance diagnostic accuracy and enable earlier intervention. Additionally, incorporating genetic and mRNA markers could provide a more comprehensive approach to early DN detection. Implementing these biomarkers in clinical practice could significantly improve outcomes for patients with DN by facilitating early diagnosis and timely management.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"104"},"PeriodicalIF":0.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Acidified Nitrite on Wound Healing in Streptozotocin-Induced Diabetic Mice.","authors":"Masoud Ghorbani, Ghazal Ghajari, Bahman Jalali Kondori","doi":"10.4103/abr.abr_115_24","DOIUrl":"10.4103/abr.abr_115_24","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus (DM) is one of the most common metabolic diseases in the world. Studies have shown that nitric oxide (NO) promotes re-epithelialization and stimulates angiogenesis and neovascularization. This study aimed to investigate the effect of exogenous NO on diabetic wound healing.</p><p><strong>Materials and methods: </strong>This study was performed on 63 male BALB/c mice. For type 2 diabetes induction, the animals were fed a high-fat diet followed by a single dose of streptozotocin (STZ) (35 mg/kg) injection intraperitoneally. Acidified nitrite cream was prepared with 3.0% (w/v) sodium nitrite (SN) and 4.5% (w/v) citric acid monohydrate, respectively, in the aqueous cream base. Histopathological examinations were performed using hematoxylin and eosin and Masson's trichrome staining.</p><p><strong>Results: </strong>The results showed that in the silver sulfadiazine-treated group, the size of the wound surface on the 7^th day was significantly (<i>P</i> < 0.05) reduced compared to the control group. There was a significant (<i>P</i> < 0.005) decrease in the size of the wound in the SN-treated group on days 7 and 14 compared to the control group. The results of histopathological studies also showed that re-epithelialization and granulation in the diabetic wound site increased in the groups treated with acidified nitrite cream compared to other groups.</p><p><strong>Conclusion: </strong>The use of topical acidified nitrite cream increases the speed of wound healing and it accelerates the healing of diabetic wounds in mice by causing a delay in the inflammation process and increasing the speed of re-epithelialization.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"97"},"PeriodicalIF":0.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calprotectin Correlates with Reduced Level of LVEF and Occurrence of Cardiac Arrhythmia in STEMI Patients.","authors":"Habib Haybar, Babak Ramezani, Ahmad Reza Assareh, Ali Kardooni, Shirin Azizidoost","doi":"10.4103/abr.abr_438_23","DOIUrl":"https://doi.org/10.4103/abr.abr_438_23","url":null,"abstract":"<p><strong>Background: </strong>Calprotectin is recognized as a promising prognostic as well as a diagnostic marker of cardiac disorders. In the present study, we aimed to survey the efficiency of serum calprotectin levels in anticipating the severity of coronary artery disease (CAD) along with in-hospital major adverse cardiovascular events (MACE) in patients with ST-segment elevation (STEMI) underlying primary percutaneous coronary intervention (PCI).</p><p><strong>Materials and methods: </strong>A total of 97 patients with STEMI participated and were evaluated for in-hospital MACE for possible correlation with serum calprotectin.</p><p><strong>Results: </strong>Increased levels of serum calprotectin showed positive and negative correlation with severity of coronary arteries and left ventricular ejection fraction (LVEF) of STEMI patients, respectively. Regarding in-hospital MACE, only arrhythmia showed a significant relationship in patients with high calprotectin levels.</p><p><strong>Conclusion: </strong>High calprotectin levels may be a prognostic marker for occluded artery and LVEF in STEMI patients.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"90"},"PeriodicalIF":0.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prevalence of PD-L1 Expression in Triple-Negative Breast Cancer Patients and Its Correlation with Survival Rates and Other Prognostic Factors: A Survival Analysis.","authors":"Arefeh Izadi, Azar Naimi, Elham Amjadi, Dorsa Beheshtiparvar, Maryam Soltan","doi":"10.4103/abr.abr_2_24","DOIUrl":"https://doi.org/10.4103/abr.abr_2_24","url":null,"abstract":"<p><strong>Background: </strong>Triple-negative breast cancer (TNBC) is a leading cause of cancer-related mortality among women, with a poor prognosis. The programmed cell death 1 (PD-1) pathway has emerged as a potential immunotherapy target. This study aimed to assess PD-L1 expression in TNBC patients and its relationship with prognostic variables.</p><p><strong>Materials and methods: </strong>This cross-sectional study included 107 TNBC patients recruited between 2016 and 2020. Patient age, tumor grade, and Ki67 expression were obtained from pathology reports. Immunohistochemistry was utilized to determine PD-L1 status, and 2-year survival data were collected through telephone follow-up.</p><p><strong>Results: </strong>PD-L1 expression frequency in TNBC patients was 76.6%. Grade 3 was the most common cancer grade, significantly more prevalent in the PD-L1 positive group (<i>P</i> = 0.01). High Ki67 expression (≥14%) was observed in 89% of patients, significantly higher in the PD-L1 positive group (<i>P</i> = 0.003). The 2-year survival rates for the PD-L1 positive and negative groups were 84.1% and 92%, respectively, with no significant difference between the groups (<i>P</i> = 0.512).</p><p><strong>Conclusion: </strong>This study investigated PD-L1 expression prevalence in TNBC patients and its correlation with prognostic variables. PD-L1 expression was associated with higher tumor grade and elevated Ki67 expression, indicating a potential role in tumor aggressiveness. However, despite these associations, PD-L1 expression did not significantly impact the 2-year survival rate in TNBC patients. These results emphasize the complexity of the immune microenvironment in TNBC and the necessity for further research to elucidate the precise role of PD-L1 in disease progression and patient outcomes.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"86"},"PeriodicalIF":0.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of <i>MYC</i> Oncogene Expression in Human Breast Cancer and Its Relationship with ACSL4 and Lipin-1 Expression.","authors":"Negar Dinarvand, Reza Azizi, Sedighe Rastaghi, Farzaneh Karimi, Abdolkarim Sheikhi, Morteza Pourfarzam","doi":"10.4103/abr.abr_419_23","DOIUrl":"https://doi.org/10.4103/abr.abr_419_23","url":null,"abstract":"<p><strong>Background: </strong>Disturbances in lipid metabolism are one of the hallmarks of cancer cells. Fatty acid synthesis and oxidation play a crucial role in the proliferation, growth, and survival of cancer cells. Several enzymes are involved in lipid metabolism. MYC is an oncogene and plays various regulatory roles in lipid metabolism. This study aimed to evaluate <i>MYC</i> expression and its association with the expressions of Lipin-1, ACSL4 (enzymes involved in lipid metabolism), in pairs of breast cancer (BC) and adjacent normal tissues to further understand the <i>MYC</i> influence on metabolic regulation.</p><p><strong>Materials and methods: </strong>Fifty-five pairs of samples of BC and noncancerous adjacent tissues were utilized in the present study to analyze <i>MYC</i>, <i>Lipin-1,</i> and <i>ACSL4</i> by quantitative real-time polymerase chain reaction. Further, the expression of Lipin-1 and ACSL4 proteins and a number of other clinicopathologically relevant variables were studied employing immunohistochemistry staining.</p><p><strong>Results: </strong><i>MYC</i> expression was substantially higher in BC tissues than in adjacent normal tissues, according to our findings. This upregulation was positively correlated with tumor size and stage. Although <i>MYC</i> expression was not correlated with that of ACSL4 and Lipin-1 expression, this may result from the complex metabolic changes that occur when cells become malignant.</p><p><strong>Conclusions: </strong>Although further research is required to assess MYC's impact on tumor metabolic regulation, the correlations seen here between <i>MYC</i>, the pathological stage, and tumor size may indicate its prognostic significance in BC. Hence, it may be considered as a potential therapeutic target for further studies.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"83"},"PeriodicalIF":0.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Directing Rat Hair Follicle Stem Cells Toward Neuronal Lineage With Enhanced Trophic Factor Expression.","authors":"Sareh Pandamooz, Sara Chavoshinezhad, Mandana Mostaghel, Armita Rasekh, Nasrin Ghorbani, Mahintaj Dara, Tahoura Pandamooz, Nader Tanideh, Mohammad Saied Salehi","doi":"10.4103/abr.abr_111_24","DOIUrl":"https://doi.org/10.4103/abr.abr_111_24","url":null,"abstract":"<p><strong>Background: </strong>Hair follicle stem cells (HFSCs) are promising candidates for cell-based therapies in neurodegenerative diseases because of their ability to differentiate into neural lineages and exert paracrine effects in damaged tissues. However, their clinical application faces challenges, particularly in efficiently guiding them toward neural lineages. This study explores using chick embryo extract (CEE) to enhance HFSCs' secretory capacity and neuronal differentiation.</p><p><strong>Materials and methods: </strong>HFSCs from rat whisker pads were cultured in growth medium supplemented with either 20% FBS or a combination of 10% FBS and 10% CEE, transitioning to 20% FBS after the first subculture. We conducted gene expression profiling of lineage commitment markers and neurotrophic factors in both experimental groups, alongside morphological assessments and protein expression analyses.</p><p><strong>Results: </strong>CEE supplementation during migration increased neuronal differentiation, evidenced by more cells with neurites and higher MAP2 expression at both the gene and protein levels. CEE also inhibited the expression of PDGFR-α, indicating a suppression of differentiation toward Schwann cells. Furthermore, we observed increased levels of trophic factors such as BDNF and VEGF at passage 3 induced by CEE supplementation.</p><p><strong>Conclusions: </strong>Enhancing the neuronal lineage commitment of hair follicle stem cells (HFSCs) and boosting the expression of trophic and angiogenic factors through short-term CEE preconditioning during their migratory stage presents a compelling approach. This strategy holds great promise in enhancing the effectiveness of stem cell-based therapies for neurological disorders.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"84"},"PeriodicalIF":0.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial Evaluation of lncRNA A2M-AS1 Gene Expression in Multiple Sclerosis Patients.","authors":"Shaghayegh Mohammadi, Tahereh Sadeghiyan, Mohammad Rezaei, Mansoureh Azadeh","doi":"10.4103/abr.abr_422_23","DOIUrl":"https://doi.org/10.4103/abr.abr_422_23","url":null,"abstract":"<p><strong>Background: </strong>Multiple sclerosis (MS) is one of the three leading neurodegenerative diseases worldwide. Gene expression profile studies play an important role in recognizing and preventing disease. Considering the inherent ability of biomarkers to diagnose and prognose the occurrence of a disease, with the aim of gene therapy and changing gene expression, it can be helped to treat it. In this study, by examining the gene interaction and expression of non-coding genes in patients with MS, using bioinformatics analyses, laboratory research and potential non-coding diagnostic biomarkers of MS were selected for further investigations.</p><p><strong>Materials and methods: </strong>First, by using micro-array data analysis of the GEO database, the expression status of the long non-coding ribonucleic acid (RNA) (lncRNA) A2M-AS1 gene was investigated in patients with MS. lncRNA-mRNA interaction analysis was performed in the lncRRisearch database. After sample collection, the total RNA extracted using the RNA extraction kit from 20 patient samples and 20 healthy samples was synthesized into cDNA with the synthesis kit. The quantitative reverse transcriptase polymerase chain reaction experiment was performed for the final validation of expression change.</p><p><strong>Results: </strong>Based on bioinformatic and laboratory analysis, the expression of the A2M-AS1 gene in MS samples showed a significant decrease in expression compared to healthy samples. Also, based on the receiver operating characteristic analysis, lncRNA A2M-AS1 can be introduced as an acceptable diagnostic biomarker to distinguish MS samples from healthy samples.</p><p><strong>Conclusion: </strong>lncRNA A2M-AS1, by reducing its expression as an acceptable diagnostic biomarker, can increase the risk of developing MS.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"80"},"PeriodicalIF":0.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}