Advanced biomedical research最新文献

{"title":"An Updated Systematic Review and Meta-Analysis of the Efficacy and Safety of Catheter Ablation in Comparison to Antiarrhythmic Drugs for Managing Atrial Fibrillation.","authors":"Ketan Garg, Paramjit K Saini, Rohan Chauhan, Kamaljit Ram Kailey, Kanika Sood, Anupamdeep Pannu, Navneet Kaur, Lovekumar Vala","doi":"10.4103/abr.abr_272_24","DOIUrl":"10.4103/abr.abr_272_24","url":null,"abstract":"<p><p>This meta-analysis compares the efficacy and safety of catheter ablation with antiarrhythmic therapy for atrial fibrillation (AF) in randomized controlled trials (RCTs) We systematically searched PubMed, Web of Science, Google Scholar, Embase, and Cochrane Library for RCTs published in English. Studies comparing catheter ablation (radiofrequency or cryoablation) with antiarrhythmics for rate or rhythm control in adults with AF were included. Data were independently extracted by two investigators. The quality of evidence was assessed using GRADE analysis. 18 RCTs involving 3725 patients were included. The overall risk of bias was adjudicated to be \"low.\" On meta-analysis, catheter ablation significantly increased the likelihood of being free from recurrent atrial arrhythmia, with a 49% reduction rate over a median follow-up of 12 months (RR: 0.51; 95% CI: 0.42-0.63; <i>P</i> < 0.00001). The risk of developing serious adverse events was similar in patients undergoing catheter ablation or receiving antiarrhythmics (RR: 0.88; 95% CI: 0.77-1.01; <i>P</i> = 0.06). However, the mortality risk over a median follow-up of 12 months was higher in patients with AF receiving antiarrhythmic drugs than in those undergoing catheter ablation (RR: 0.74; 95% CI: 0.58-0.94; <i>P</i> = 0.01). The evidence was found to be of \"high\" quality on GRADE analysis. It was concluded that catheter ablation is more effective than antiarrhythmic drugs in managing AF due to a lower risk of recurrence. Although it has a similar likelihood of serious adverse effects, the mortality risk is lower over a median follow-up of 12 months.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"23"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occupational Stress in Pre-Hospital Emergency Care: A Review on Influencing Factors and Consequences and Management: A Systematic Review.","authors":"Mehdi Safarabadi, Masoud Etedali, Paria Bahrami, Davood Azadi, Ali Jadidi","doi":"10.4103/abr.abr_222_24","DOIUrl":"10.4103/abr.abr_222_24","url":null,"abstract":"<p><p>Occupational stress is a significant concern in the field of pre-hospital emergency care, affecting the well-being of healthcare professionals and the quality of patient care. This review aims to explore the influencing factors, consequences, and management strategies associated with occupational stress in this specific setting. This systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. A literature search was conducted using relevant databases, including PubMed, Scopus, and Web of Science. Studies published within the last decade were included, with a focus on empirical research and systematic reviews. The eligibility of the studies was evaluated based on predetermined inclusion and exclusion criteria. The review identified several influencing factors contributing to occupational stress in pre-hospital emergency care, such as high workload, time pressure, exposure to traumatic events, organizational factors, and personal characteristics. The consequences of occupational stress encompassed burnout, decreased job satisfaction, compromised patient care, increased absenteeism, and higher turnover rates. Various management strategies were explored, including workload management, shift scheduling optimization, creating a supportive work environment, and stress management training. The findings highlight the need for effective management of occupational stress in pre-hospital emergency care. Addressing the influencing factors and implementing appropriate management strategies can mitigate the consequences of occupational stress and enhance the well-being of healthcare professionals. This review provides valuable insights for healthcare organizations, policymakers, and practitioners in developing interventions and policies to manage occupational stress and improve the overall quality of care in pre-hospital emergency settings. Further research is warranted to evaluate the effectiveness of specific interventions and develop tailored approaches to address occupational stress in this context.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"32"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Effect of Caffeine on the Effectiveness of Crocin in Improving Behavioral Symptoms and Brain Tissue Changes in the Hippocampus in the Post-Traumatic Stress Model in Rats.","authors":"Mohammadmehdi Alfideh, Gila Pirzad Jahromi, Gholam Reza Kaka, Mohsen Khodadadi, Yousef Alimohamadi","doi":"10.4103/abr.abr_464_25","DOIUrl":"10.4103/abr.abr_464_25","url":null,"abstract":"<p><strong>Background: </strong>Post-traumatic stress disorder (PTSD) is a debilitating psychiatric condition. Although crocin has shown potential in reducing stress-related symptoms, its low bioavailability limits its effectiveness. Caffeine may enhance the bioavailability of various compounds by modulating liver enzymes. This study examined whether caffeine could improve crocin's efficacy in a rat model of PTSD.</p><p><strong>Materials and methods: </strong>Male Wistar rats (8-10 weeks old) were divided into five groups: control, PTSD, crocin-treated, caffeine-treated, and crocin + caffeine-treated. PTSD was induced using repeated foot shocks, followed by 7 days of treatment. Behavioral assessments were performed using the elevated plus maze and shuttle box tests. Serum corticosterone levels were measured as a biochemical marker of anxiety, and histological evaluation of the hippocampus was conducted using Cresyl Violet and Golgi-Cox staining.</p><p><strong>Results: </strong>The combination treatment significantly reduced serum corticosterone levels compared to either compound alone (<i>P</i> < 0.01). Behavioral improvements-including increased grooming, time spent in open arms, and reduced freezing latency-were most pronounced in the crocin + caffeine group. Histological analysis also revealed reduced neuronal damage in this group.</p><p><strong>Conclusions: </strong>We concluded that a combination of crocin and caffeine could improve the efficacy of crocin given alone. These findings indicate that caffeine can help further enhance the neuroprotective efficacy of crocin in this model.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"33"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing the Effect of Extracorporeal Shockwave Therapy after Botulinum Toxin Injection on Calf Muscle Spasticity Improvement in Patients with Multiple Sclerosis - A Randomized Clinical Trial.","authors":"Shila Haghighat, Marjan Ghasri, Babak Vahdatpour, Vahid Shaygannejad","doi":"10.4103/abr.abr_671_24","DOIUrl":"10.4103/abr.abr_671_24","url":null,"abstract":"<p><strong>Background: </strong>Spasticity is a frequent and disabling manifestation of multiple sclerosis (MS), characterized by increased muscle tone and stiffness that limit mobility and daily functioning. Effective management requires a multimodal therapeutic strategy. This randomized clinical trial evaluated the efficacy of adjunct extracorporeal shockwave therapy (ESWT) following botulinum toxin type A (BTX-A) injection in reducing calf muscle spasticity in MS patients.</p><p><strong>Materials and methods: </strong>Twenty participants were randomly assigned to receive either BTX-A alone (n = 10) or BTX-A combined with ESWT (n = 10). Clinical outcomes, including range of motion (ROM), Modified Ashworth Scale, and pain assessed by visual analog scale (VAS), were measured at 10 days, 1 month, and 2 months post-treatment.</p><p><strong>Results: </strong>Dorsiflexion ROM showed no significant between-group difference (<i>P</i> = 0.807), whereas plantar-flexion ROM significantly improved in the BTX-A + ESWT group compared with BTX-A alone (<i>P</i> = 0.047). MAS scores declined significantly over time in both groups (<i>P</i> < 0.001) without intergroup difference. Pain reduction, assessed by VAS, was greater in the ESWT group, showing significant between-group differences (<i>P</i> = 0.02). Compared with BTX-A alone, adjunct ESWT produced greater VAS improvement at day 10 (mean difference - 0.90, 95% CI - 1.65 to - 0.15) and 1 month (-1.10, 95% CI - 1.76 to - 0.44).</p><p><strong>Conclusions: </strong>ESWT as an adjunct to BTX-A enhances pain relief and improves plantar-flexion ROM in MS-related calf spasticity compared with BTX-A alone, supporting its role as a valuable component of multimodal spasticity management.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"29"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Combined Vitamin D and A Supplementation on Clinical Outcomes in Patients with Pneumosepsis: A Randomized Double-Blind Clinical Trial.","authors":"Abolfazl Jokar, Alireza Sistani, Farshideh Didgar, Elham Niasati","doi":"10.4103/abr.abr_271_24","DOIUrl":"10.4103/abr.abr_271_24","url":null,"abstract":"<p><strong>Background: </strong>Pneumosepsis is a severe and potentially life-threatening infection of the lower respiratory tract. This study aimed to investigate the effect of combined vitamin D and A supplementation on clinical outcomes in patients with pneumosepsis.</p><p><strong>Material and methods: </strong>Eighty-four patients with pneumosepsis were randomly assigned to four groups in this double-blind clinical trial. Group 1 received standard treatment plus 300,000 IU intramuscular vitamin D and 50,000 IU intramuscular vitamin A. Group 2 received standard treatment plus 300,000 IU intramuscular vitamin D. Group 3 received standard treatment plus 50,000 IU intramuscular vitamin A. Group 4 received standard treatment only. Clinical parameters including ESR, CRP, creatinine, urea, and uric acid levels were recorded at baseline, 24, 48, and 72 hours after intervention.</p><p><strong>Results: </strong>The combined vitamin D and A group (Group 1) demonstrated significantly lower blood pressure, respiratory rate, heart rate, temperature, ESR, CRP, and white blood cell count compared to the other groups at all three time points.</p><p><strong>Conclusion: </strong>The study suggests that combined vitamin D and A supplementation, in addition to standard treatment, may improve clinical outcomes and potentially reduce the duration of hospitalization in patients with pneumosepsis. However, further research is warranted to confirm these findings and elucidate the underlying mechanisms.</p><p><strong>Trial registration: </strong>This study is registered on Iranian Registry of Clinical Trials (IRCT) under the identifier IRCT20130424013110N10. (The registration date: July 6, 2020).</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"26"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency of Pulmonary Embolism in Patients with COPD Exacerbation and Clinical and Laboratory Predictive Factors.","authors":"Shahrzad Keymanesh, Somayeh Sadeghi","doi":"10.4103/abr.abr_219_24","DOIUrl":"10.4103/abr.abr_219_24","url":null,"abstract":"<p><strong>Background: </strong>Chronic obstructive pulmonary disease (COPD) is the leading cause of respiratory morbidity and mortality. Patients with COPD have an increased risk of pulmonary thromboembolism (PTE), which complicates clinical management. This study aimed to evaluate the frequency of PTE in patients with COPD exacerbations and to identify clinical and laboratory predictive factors associated with PTE.</p><p><strong>Materials and methods: </strong>A retrospective cross-sectional study was conducted at Al-Zahra Hospital, Isfahan, Iran, between 2021-2022. Patients diagnosed with COPD exacerbation and shortness of breath were assessed for PTE by using computed tomography pulmonary angiography. Clinical data, including demographics, blood gas levels, and ECG and echocardiography findings, were collected and analyzed using SPSS 24 software.</p><p><strong>Results: </strong>Among the 260 COPD patients, 26 (10%) were diagnosed with PTE. Patients with PTE exhibited significantly higher body mass index (BMI), smoking and D-dimer levels, and lower oxygen saturation. Logistic regression analysis identified older age, BMI, smoking, and abnormal electrocardiogram (ECG) as independent predictors of PTE (<i>P</i> < 0.001 for all).</p><p><strong>Conclusion: </strong>This study highlights the notable prevalence of PTE in patients with COPD exacerbations. Factors such as obesity, smoking, and abnormal ECG findings are critical in predicting the risk of PTE. These findings underscore the necessity for vigilant assessment and management of PTE in COPD patients to improve clinical outcomes in this vulnerable population.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"25"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics Analysis of the miR-15 Family Members and Their Potential Target Genes in Breast Cancer Progression.","authors":"Mahdi Alizadeh, Mahdieh Salimi","doi":"10.4103/abr.abr_634_24","DOIUrl":"10.4103/abr.abr_634_24","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is the most common cancer in women and the second most frequent cancer worldwide. Changes in miRNA levels are associated with various diseases, including cancer, making them ideal non-invasive biomarkers for diagnosis, prognosis, and treatment. Bioinformatics advancements have enabled the prediction of miRNAs and their target genes. This study aimed to analyze the miR-15 family (miR-15a, miR-15b, miR-16-1, miR-16-2, miR-195, and miR-497) to predict their target genes and evaluate their role in breast cancer progression.</p><p><strong>Materials and methods: </strong>Expression levels of the miR-15 family were evaluated in healthy and cancerous breast tissues using the OncomiR database. Target genes involved in breast cancer-related pathways were identified through the DIANA TOOLS-mirPath v.3 database. Algorithms from online databases such as TargetScan, miRWalk 2.0, and RNAhybrid were used to rank the genes. Genes with the highest scores were analyzed for their functional roles using the DAVID database.</p><p><strong>Results: </strong>Analysis of cell adhesion, TGF-beta, and P53 pathways identified seven genes (IGF1R, PVRL1, SMAD7, CCND2, CCND1, SESN1, and CCNE1) as common targets of miR-15a-5p, miR-15b-5p, miR-195-5p, and miR-497-5p. Additionally, CCNG2 was identified as an exclusive target of miR-16-1-3p. These genes were enriched in pathways associated with cell adhesion, apoptosis, and key cancer-related signaling networks.</p><p><strong>Conclusion: </strong>Members of the miR-15 family may serve as biomarkers for breast cancer by targeting genes involved in cell adhesion, apoptosis, and pathways such as TGF-β and P53. Further clinical and laboratory studies are recommended to validate these findings.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"31"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-<i>Toxoplasma</i> Activity of Copper Nanoparticles (CuNPs) Synthesized Using <i>Conocarpus erectus</i> L.: An <i>In Vitro</i> and <i>In Vivo</i> Study.","authors":"Bahram Rostami, Hamid Hasanpour, Sanaz Jafarpour Azami, Mona Roozbehani, Saeideh Hashemi-Hafshejani, Omid Raiesi, Muhammad I Getso, Alireza Badirzadeh, Shahab Falahi, Reza Pakzad, Mohammad Davoodzadeh Gholami, Hesam Yousefi, Razi Naserifar","doi":"10.4103/abr.abr_401_23","DOIUrl":"10.4103/abr.abr_401_23","url":null,"abstract":"<p><strong>Background: </strong>This study evaluates <i>in vitro</i> and <i>in vivo</i> anti-<i>Toxoplasma</i> effects of copper nanoparticles (CuNPs) green synthesized using <i>Conocarpus erectus</i> L.</p><p><strong>Materials and methods: </strong>CuNPs were green synthesized using the aqueous extract of <i>Conocarpus erectus</i> L. An MTT assay was performed on Hella cells to evaluate the cell viability. The <i>in vitro</i> anti-<i>Toxoplasma</i> activity of various concentrations of CuNPs (20-160 ppm) for 1, 2, 4, and 8 h at room temperature was assessed against the <i>Toxoplasma gondii</i> RH tachyzoites. Moreover, the flow cytometry test was carried out to confirm the results. For <i>in vivo</i> assessment, BALB/c mice were infected with RH strains, subsequently treated with CuNPs, and compared with the control group.</p><p><strong>Results: </strong>CuNPs had a particle size of less than 20 nm, with a maximum peak at 315 nm, according to transmission electron microscopy. The highest mortality rate was observed at 160 ppm concentration, and after 8 h of exposure, it was demonstrated by the result of flow cytometry. Furthermore, oral administration of CuNPs increased oral bioavailability and mice survival time and reduced parasitemia. Green synthesized CuNPs by <i>Conocarpus erectus</i> L. reduced the tachyzoites of <i>Toxoplasma gondii</i>, RH strain <i>in vitro</i>, and increased the survival time of infected mice treated with CuNPs compared to the control group.</p><p><strong>Conclusions: </strong>To achieve effective treatment against toxoplasmosis, it is suggested that more studies will be done on other strains, especially type II. Finally, it seems that the use of CuNPs can be helpful as a supplementary treatment alongside common treatments.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"30"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncolytic Effects of S89K Matrix Protein of Vesicular Stomatitis Virus in Cervical Cancer.","authors":"Mehdi Ajorloo, Ashkan Alamdary, Samira Samaie, Reza Arabi Mianroodi, Behzad Esfandiari, Seyed M Hasanzadeh, Alireza Gholami","doi":"10.4103/abr.abr_327_25","DOIUrl":"10.4103/abr.abr_327_25","url":null,"abstract":"<p><strong>Background: </strong>Oncolytic viruses are currently the subject of cancer treatment research. Unlike other viruses, they can target tumor cells while avoiding healthy ones. The vesicular stomatitis virus (VSV) has the ability to kill tumor cells through one of two different apoptotic pathways, depending on which cell line is being studied. Even when no other viral components are present, the VSV matrix protein can induce cell death in HeLa cells through apoptosis. The purpose of this research was to examine the similarities and differences in the induction of cell death by native and mutant VSV matrix proteins.</p><p><strong>Materials and methods: </strong>To create the mutant VSV matrix protein, the amino acid Ser was substituted with Lys at residue 89 (S89K). After the matrix gene was cloned into the expression vector, both the normal and mutant versions were transfected into HeLa cells. The expression was verified by fluorescence microscopy, and flow cytometry was used to evaluate the apoptosis rate.</p><p><strong>Results: </strong>After 48 hours of transfection, the VSV matrix protein was found to promote cell death at the highest level. The results showed that once basic amino acids were substituted with alcoholic ones in the S89K mutant, the apoptotic activity of the VSV matrix protein was enhanced.</p><p><strong>Conclusion: </strong>This study discovered that increasing apoptosis induction by introducing a specific mutation at a specific location in the VSV matrix protein improved its apoptotic capabilities. The ability to engineer recombinant viruses with specific mutations is crucial for the development of cancer vaccines that target specific cell lines.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"28"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing Drug Delivery: The Power of Ultrasound in Ameliorative Effects of Functionalized Single-Walled Carbon Nanotube Encapsulated Dacarbazine in a Murine B16F10 Melanoma Model.","authors":"Moghadaseh Mahdinejad, Mohammad Bagher Tavakoli, Azade Taheri, Fateme Heidari, Seyed Hossein Hejazi, Tahereh Komeili Movahed, Hadi Nasrollahi","doi":"10.4103/abr.abr_455_23","DOIUrl":"10.4103/abr.abr_455_23","url":null,"abstract":"<p><strong>Background: </strong>The field of drug delivery has witnessed remarkable advancements aimed at optimizing therapeutic outcomes and reducing adverse effects. In this study, we examined the ameliorative effect of ultrasound waves in combination with Functionalized Carbon nanotubes -encapsulated Dacarbazine (fSWCNT-DTIC) in a murine B16F10 melanoma model.</p><p><strong>Materials and methods: </strong>The Preparation of single-walled carbon nanotube encapsulated Dacarbazine (fSWCNT-DTIC) accrued at several steps. The melanoma tumor model was induced by injection of B16F10 cells subcutaneously into the right shoulder of c57bl/6 mice. After the tumor reached a volume of 100mm³each group was treated locally with 60 μL of either vehicle (normal saline), Dacarbazine, Carbon nanotube, Ultrasound, or a combination of them. Tumor Growth inhibition, systemic toxicity, and also liver and lung histopathological changes were assayed in different groups. In all analyses, a significance level of 0.05 was deemed appropriate.</p><p><strong>Result: </strong>The highest Tumor Growth inhibition (%88.21) was seen in the group that received single-walled carbon nanotubes encapsulated Dacarbazine and also exposed to ultrasound radiation. In various groups, there was no noticeable variance in weight loss at the experimental period. Combination therapy improved the chaotic condition, inhibited the spread of cancer cells in the lungs, reduced severe inflammation in the lung lobes and reversed abnormal patterns in the liver.</p><p><strong>Conclusion: </strong>The combination of ultrasound and SWCNT-encapsulated Dacarbazine, could make a significant difference in the treatment process and improve the treatment outcomes for cancer patients.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"21"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}