Advanced biomedical research最新文献

{"title":"Evaluation of Surface EMG in Masticatory, Sternomastoid, and Trapezius Muscles among Smartphone Users with Varying Duration of Mobile Usage: An Observational Cross-Sectional Study.","authors":"Avinash E Thakare, Pooja S Salkar, Sandip M Hulke, Santosh L Wakode, Rajay N Bharshankar, Anshul Rai","doi":"10.4103/abr.abr_288_24","DOIUrl":"10.4103/abr.abr_288_24","url":null,"abstract":"<p><strong>Background: </strong>The overuse of smartphones can cause and trigger painful musculoskeletal conditions due to the derangement of muscle function. This study aimed to investigate the impact of daily smartphone usage on the surface electromyographic activity of the masticatory, sternomastoid, and trapezius muscles as there is limited research in this area.</p><p><strong>Materials and methods: </strong>A total of 120 young adult smartphone users were categorized into three groups based on their daily duration of mobile usage. They underwent surface electromyography (sEMG) assessment using the Neuropack X1 surface EMG device from Nihon Kohden. Using appropriate statistical methods, the sEMG parameters were compared and correlated within the three groups.</p><p><strong>Results: </strong>The study found a significant increase in resting sEMG activity in the right Trapezius muscle among smartphone users who used their devices for more than 7 hours daily, compared to the other user groups (median interquartile range: 0.49 (0.026, 0.161) vs 0.025 (0.014, 0.049)). Furthermore, the maximum voluntary contraction testing revealed significantly higher sEMG activity in the right Temporalis muscle among smartphone users who used their devices for more than 7 hours daily, compared to the other user groups (median interquartile range: 2.342 (0.972, 3.364) vs 1.391 (0.525, 2.732) vs 1.301 (0.482, 2.083)).</p><p><strong>Conclusions: </strong>The findings suggest that the daily duration of smartphone usage could potentially impact the surface EMG activity of the masticatory, neck, and upper back muscles.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"54"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Insight into Pathogenic Variant p.L275P on the Structure and Function of Steroidogenic Acute Regulatory Protein (STAR) Through Molecular Dynamics Simulation.","authors":"Shahrzad Aghaei, Mohammad Amin Tabatabaiefar, Javad Saffari-Chaleshtori, Erfan Korram, Sara Sadeghi Amini, Maryam Aghaei, Mahbobeh Koohiyan, Zahra Ataie, Payam Samei, Effat Farrokhi","doi":"10.4103/abr.abr_439_23","DOIUrl":"10.4103/abr.abr_439_23","url":null,"abstract":"<p><strong>Background: </strong>Lipoid congenital adrenal hyperplasia (LCAH) is the lethal form of steroid biosynthesis defects because of mutations in the steroidogenic acute regulatory (<i>STAR</i>) gene. There are two distinct clinical forms including classical and nonclassical without any reliable genotype-phenotype correlation, and investigation of more cases may provide valuable insights.</p><p><strong>Materials and methods: </strong>Ten Iranian families affected by LCAH were selected for genetic linkage analysis. The <i>STAR</i> gene was sequenced in the linked families, and using multiple <i>in silico</i> tools and ACMG guidelines, the pathogenicity of candidate variants was assessed. Also, the structural effects of the identified variant on the STAR behavior were evaluated using molecular docking and molecular dynamics (MD) simulation.</p><p><strong>Results: </strong>Data analysis revealed a pathogenic variant, c.824T>C, (p.L275P), in the <i>STAR</i> gene in the Iranian population for the first time. Furthermore, molecular docking and MD simulation revealed that the c.824T>C variant results in a decrease in total flexibility and secondary structure, an increase in H-bonds and changed fluctuations in some residues.</p><p><strong>Conclusions: </strong>MD simulation provides the key structural and structural insights of the p.L275P -mediated conformational changes in STAR.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"46"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>EZH2</i>: A Crucial Competing Endogenous RNA in Cancer Research-A Scoping Review.","authors":"Sadra Salehi-Mazandarani, Sharareh Mahmoudian-Hamedani, Ziba Farajzadegan, Parvaneh Nikpour","doi":"10.4103/abr.abr_561_24","DOIUrl":"10.4103/abr.abr_561_24","url":null,"abstract":"<p><p>Recently, research on the competing endogenous RNAs (ceRNAs) in cancer has been in full swing, emphasizing their importance as critical RNAs in cancer progression. Enhancer of zeste 2 polycomb repressive complex 2 subunit (<i>EZH2</i>) is a ceRNA that has been introduced as a potential therapeutic target in many cancers. Due to EZH2's dual role as an oncogene and tumor suppressor in cancer, a more thorough exploration of its ceRNA functions may enhance clinical cancer treatment approaches. In the current scoping review, we searched several online databases to identify experimentally validated ceRNA axes, including <i>EZH2</i> in human cancers. We identified 66 unique axes consisting of 30 microRNAs (miRNAs), 32 long non-coding RNAs (lncRNAs), 9 messenger RNAs (mRNAs), and 14 circular RNAs (circRNAs). Notably, <i>SPRY4-IT1 -</i> miR-101-3p - <i>EZH2</i> and <i>XIST -</i> miR-101-3p - <i>EZH2</i> were recurrent axes observed in multiple cancer types. Among the most frequent miRNAs were miR-101-3p, miR-144-3p and miR-124-3p, and ceRNAs including <i>SPRY4-IT1</i>, <i>XIST</i>, <i>SNHG6</i>, <i>HOXA11-AS</i>, <i>MALAT1</i>, and <i>TUG1</i> emerged as frequent competitors of <i>EZH2</i> for miRNA binding. This scoping review highlights the diversity of <i>EZH2</i>-containing ceRNA axes in cancer, suggesting their potential as therapeutic targets. Further studies are needed to clarify their roles and clinical utility.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"53"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum β-hCG, AMH, TSH, and PRL Levels in Predicting the Outcome of Early Pregnancy 14 Days after Embryo Transfer: A Prospective Cohort Study.","authors":"Fatemeh Davari Tanha, Azam Rasti, Hamideh Pakniat, Shohreh Salimi Setudeh, Amir Hosseini, Mohammad Amin Rahimian Ghohroodi","doi":"10.4103/abr.abr_285_23","DOIUrl":"10.4103/abr.abr_285_23","url":null,"abstract":"<p><strong>Background: </strong>Pregnancy outcomes in assisted reproductive technology (ART) are influenced by the ability of the embryos to create a pregnancy and maternal factors such as hormone levels to maintain and develop the pregnancy. This study investigated the role of β-human chorionic gonadotropin (β-hCG), anti-mullerian hormone (AMH), thyroid-stimulating hormone (TSH), and prolactin (PRL) in predicting pregnancy outcomes and determined cutoff values in women undergoing ARTs.</p><p><strong>Materials and methods: </strong>This prospective study included 153 women who underwent infertility treatment in the Department of Reproductive Medicine of the Yas Hospital, Tehran University of Medical Sciences (Tehran, Iran). Serum β-hCG level on day 14 after embryo transfer and serum AMH, TSH, and PRL were measured. Patients with β-hCG levels >25 mIU/ml were included in the study. Finally, any statistically significant relationships between these serum values and clinical pregnancy and live birth rate were investigated. To predict pregnancy outcomes with β-hCG levels, a receiver operating characteristic curve was used to estimate the β-hCG cutoff values.</p><p><strong>Results: </strong>Serum β-hCG levels were significantly higher in live-birth patients than in other patients. The β-hCG cutoff value for live births was 830 mIU/ml, with an AUC of 0.5920, sensitivity of 61.04%, and specificity of 56.58%. Serum AMH, TSH, and PRL levels did not show any significant results.</p><p><strong>Conclusions: </strong>Serum β-hCG levels on day 14 after embryo transfer can be used to predict the probability of live birth in patients undergoing infertility treatment.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"45"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-216 Is a Key Regulator and Potential Marker in Human Cancers.","authors":"Mozhgan Mondeali, Fatemeh Mozaffari, Pooran Ghiasizadeh, Sanaz Bakhti, Marjan Salehi, Navid Kamali, Milad Rafat","doi":"10.4103/abr.abr_184_23","DOIUrl":"10.4103/abr.abr_184_23","url":null,"abstract":"<p><p>MicroRNAs, a class of small noncoding RNAs, have been identified as promising biomarkers for cancer identification and management by regulating gene expression and other cellular biological pathways. This review gathers findings for understanding the molecular basis and clinical importance of microRNA-216 (miR-216) in several cancers. Increased or decreased expression of miR-216 has been observed in a variety of cancers, including esophageal cancer, breast cancer, colorectal cancer, gastric cancer, pancreatic cancer, cervical cancer, brain tumor (glioma), prostate cancer, and acute myeloid leukemia, indicating its activity as an oncogene or tumor suppressor. Through this study, we proposed that miR-216 can potentially be a candidate as a prognostic marker for early detection of tumor development, progression, as well as metastasis in cancer patients.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"40"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Prevalence and Risk Factors for Infection Following Total Hip Arthroplasty in Traumatic Femoral Neck Fracture Patients: A Retrospective Cohort Study.","authors":"Mehdi Teimouri, Elham Shahbazi, Hasan Taheri Ghale Taki, Mahta Noorbakhsh Dehkordy, Milad Ghaderi, Amir Mohammad Taravati, Morvarid Tabbakh Jashni","doi":"10.4103/abr.abr_127_23","DOIUrl":"10.4103/abr.abr_127_23","url":null,"abstract":"<p><strong>Background: </strong>Infection is one of the most difficult complications after total hip arthroplasty (THA). This study was designed to investigate the prevalence of infection after total hip arthroplasty in traumatic femoral neck fracture patients.</p><p><strong>Materials and methods: </strong>This retrospective cohort study was performed in 2014-2018 on 441 patients who underwent total hip arthroplasty emergency following traumatic femoral neck fracture and the prevalence of infection was evaluated. Demographic data were collected from the patient's medical documents. The related risk factors include diabetes, body mass index (BMI), chronic kidney disease (CKD), ischemic heart disease (IHD), hypertension (HTN), rheumatoid arthritis (RA), intravenous (IV) drug abuse, duration of surgery, amount of bleeding during surgery, and also taking or not taking prophylactic antibiotics were collected and analyzed.</p><p><strong>Result: </strong>In the present study, among 441 patients 24 of them had infection after total hip arthroplasty. The prevalence of infection was 5.4%. This study showed that gender (<i>P</i> = 0.85), age (<i>P</i> = 0.56), duration of surgery (<i>P</i> = 0.23), bleeding rate (<i>P</i> = 0.78), HTN (<i>P</i> = 0.27), IHD (<i>P</i> = 0.11), RA (<i>P</i> = 0.19), IV drug abuse (<i>P</i> = 0.054) did not increase the rate of infection after THA. Diabetes (<i>P</i> = 0.01), BMI (<i>P</i> < 0.001), CKD (<i>P</i> = 0.008), and the presence of underlying diseases (<i>P</i> < 0.001) had a significant relation with infection followed by THA.</p><p><strong>Conclusion: </strong>In this study, diabetes, BMI, CKD, and the presence of underlying diseases are the most common risk factors for increasing the risk of infection after THA. There was no significant correlation between the prevalence of infection and the following factors, gender, age, duration of surgery, amount of bleeding, HTN, IHD, RA, and IV drug abuse.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"41"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic Ag-Cu Nanoparticles Synthesized with Extract of <i>Eryngium Billardieri</i> L. and Evaluation of their Anticancer Potential on PC-3 and LNCaP Cancer Cell Lines.","authors":"Zahra Ebrahim, Ghazaleh Feyzabadi, Nastaran Asghari Moghaddam, Azadeh Mohammadgholi","doi":"10.4103/abr.abr_255_24","DOIUrl":"10.4103/abr.abr_255_24","url":null,"abstract":"<p><strong>Background: </strong>The anticancer action of biogenic Ag-Cu nanoparticles (NPs) using <i>Eryngium billardieri</i> against PC-3 and LNCaP cell lines in prostate malignancy was investigated.</p><p><strong>Materials and methods: </strong>XRD, FTIR, EDAX, FESEM, and TEM were used to illustrate the Ag-Cu NPs. The PC-3 and LNCaP cell lines were used to test the anticancer ability of Ag-Cu NPs. The cytotoxicity was assessed by MMT and flow cytometry. Apoptotic and metastatic-promoting gene expressions were evaluated by real-time PCR. ROS generation was measured.</p><p><strong>Results: </strong>NPs are spherical and their average size was 12 ± 9.7 nanometers. The EDAX result indicated the presence of Ag, Cu, and C. The XRD results revealed the NPs' crystalline structure. The results demonstrated a remarkable antiproliferative effect of the nanoparticles, with IC50 values of 15.43 μg/mL for PC-3 and 7.64 μg/mL for LNCaP. The Ag-Cu NPs exhibited a tendency to trigger apoptosis. This was confirmed by analyzing apoptosis-related gene expression. The apoptotic influence of Ag-Cu NPs was suggested to be critical when compared to the control and extract-treated groups by the up-regulation of <i>Bcl2</i>-related X (<i>Bax</i>), <i>caspase3</i>, and <i>caspase9</i>, and the down-regulation of <i>Bcl2</i>. Furthermore, Annexin V-FITC/propidium iodide presented a 23.15% and 22.3% apoptotic ratio of PC-3 and LNCaP cells, respectively. Vascular endothelial growth factor (<i>VEGF</i>), and metalloproteinases (MMPs) down-regulation showed that Ag-Cu NPs were hostile to metastasis. Also, ROS generation was estimated at 1261 and 1366 RFU in LNCaP and PC-3 cells treated with green-synthesized Ag-Cu NPs, respectively.</p><p><strong>Conclusions: </strong>The study suggested <i>E. billardieri</i> synthesized Ag-Cu as a potential candidate in prostate cancer therapeutic management.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"43"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Local Subcutaneous Infiltration with Ropivacaine Alone and Two Different Doses of Dexmedetomidine Plus Ropivacaine for Postoperative Pain after Cesarean Section Under Spinal Anesthesia.","authors":"Atefeh Ghosouri, Alireza Hoghooghy, Azim Honarmand, Arefeh Kabirzadeh","doi":"10.4103/abr.abr_253_24","DOIUrl":"10.4103/abr.abr_253_24","url":null,"abstract":"<p><strong>Background: </strong>In a cesarean section (CS), good postoperative pain management is of great importance. We assessed the effect of adding dexmedetomidine to ropivacaine 0.5% in CS patients with spinal anesthesia to assess its ability to enhance pain management.</p><p><strong>Materials and methods: </strong>This was a randomized controlled trial in Shahid Beheshti Hospital between 2022 and 2023 with the goal of assessing ropivacaine efficacy with and without dexmedetomidine on pain control after a CS. Spinal anesthesia was administered for all patients by using bupivacaine 0.5%. Before wound closure, patients were treated with analgesics. Group A received dexmedetomidine 1 µg/kg infiltrated subcutaneously, followed by ropivacaine 0.5% 3 mg/kg diluted with normal saline to 40 cc. Group B received dexmedetomidine 2 µg/kg, followed by ropivacaine 0.5% 3 mg/kg. The control group received ropivacaine 0.5% 3 mg/kg. A <i>P</i> value of < 0.05was regarded as significant.</p><p><strong>Results: </strong>The study groups exhibited significantly lower visual analog scale (VAS) pain scores. Specifically, group A showed significantly lower VAS scores than the controls, and group B also showed significantly lower VAS scores than group A. The VAS score after 24 hours of CS was, on average, 2.13 (SD = 0.16, 95% CI = [1.74, 2.52]) lower in group B compared to the control group and 1.1 (SD = 0.16, 95% CI = [0.71, 1.49]) lower than in group A (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>Subcutaneous infiltration of dexmedetomidine with ropivacaine improves postoperative pain management more effectively than using ropivacaine alone. It is noteworthy that dexmedetomidine 2 µg/kg can improve pain after a CS more effectively than 1 µg/kg.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"52"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Effect of Secretome Secreted from Adult Rat Kidney-Derived Stem Cells on the Expression of miR375, miR494 and Tissue Changes in Glycerol-Induced Acute Kidney Injury.","authors":"Armita Valizadeh Gorji, Fereshtesadat Fakhredini, Fatemeh Soltanpour, Esrafil Mansouri","doi":"10.4103/abr.abr_338_24","DOIUrl":"10.4103/abr.abr_338_24","url":null,"abstract":"<p><strong>Background: </strong>Acute kidney injury (AKI) causes high mortality. Cell therapy has provided acceptable results, but factors such as immune rejection and teratoma formation have limited their use. The purpose of this research is to study the reparative effects of secretome secreted from kidney-derived stem cells on glycerol-induced kidney damage in rats.</p><p><strong>Materials and methods: </strong>Secretomes were isolated from conditioned media of kidney mesenchymal stem cells. The animals were separated into 4 groups: Control, Secretome (received 30 μg/kg secretome, for 3 days), Glycerol (10 ml/kg of glycerol, intramuscularly) and Glycerol + Secretome (received glycerol and secretome). Blood urea (BUN), Creatinine (Cr) and Albumin (alb) levels were measured with detection kits. The histopathological results of the secretomes on kidney were checked with PAS staining and the amount of expression of miR-375 and miR-494 was assessed by quantitative real-time polymerase chain reaction (qRT-PCR).</p><p><strong>Results: </strong>The results of this research showed that intravenous of secretomes significantly decreased the levels of BUN (<i>P</i> < 0.01), Cr (<i>P</i> < 0.001), and alb (<i>P</i> < 0.001). Well as there was a considerable decreased in miR-375 (<i>P</i> < 0.001) and miR-494 (<i>P</i> < 0.01) in the receiving group of Glycerol + Secretome rats. The results showed secretomes improved kidney histopathology by inducing regulatory changes in the expression levels of microRNAs (miRNAs).</p><p><strong>Conclusion: </strong>The results of this research showed that secretomes extracted from kidney stem cells can play a role in reducing kidney damage by regulating miRNAs related to AKI.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"48"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nebulization of Mesenchymal Stem Cells Secretome Attenuates Acute Respiratory Distress Syndrome Caused by SARS-CoV-2 Corona Virus: An Open-Label, Controlled Trial.","authors":"Masoumeh Baghalishahi, Maysam Yousefi, Seyed Noureddin Nematollahi-Mahani, Mahsa Ziasistani, Fahimeh Pourjafari, Mona Saheli","doi":"10.4103/abr.abr_477_23","DOIUrl":"10.4103/abr.abr_477_23","url":null,"abstract":"<p><strong>Background: </strong>Previous investigations have indicated the potential of mesenchymal stem cell (MSC) transplantation in treating COVID-19. However, the limited lifespan of transplanted cells constrains this therapeutic approach. the immunomodulatory, anti-inflammatory, and regenerative properties of MSCs secretome could be a promising solution to improve patient outcomes.</p><p><strong>Materials and methods: </strong>A randomized clinical trial was conducted in Kerman, a region in south-central Iran. This study enrolled twenty patients in the severe phase of COVID-19 pneumonia, randomly allocated into either investigation or control groups. Both groups received standard therapy, the investigation group additionally received nebulized concentrated MSCs secretome, 2 mL administered twice daily for five consecutive days. To evaluate the effect of MSCs secretome therapy, the length of stay in the intensive care unit (ICU), oximetry, and paraclinical results were assessed at baseline and 5 days after ICU admission.</p><p><strong>Results: </strong>Baseline characteristics were balanced and distributed among the two groups. No adverse effect was observed in MSCs secretome-treated patients. Compared to the control group, the investigated group exhibited a decrease in ICU stay duration (10.90 ± 3.75 vs. 5.50 ± 1.64 days, <i>P</i> = 0.04) and a significant improvement in O2 saturation (60.65 ± 16.58 vs. 79.47 ± 15.36, <i>P</i> = 0.02). In addition, significant decreases in CRP, PTT, and INR and upregulation in lymphocyte fraction were observed in the investigation group during the study period.</p><p><strong>Conclusions: </strong>The findings of this study underscore the prospective advantages and safety of the secretome of MSCs as a viable therapeutic choice in COVID-19 patients. We advise verifying our results through additional clinical trials at various disease stages.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"44"},"PeriodicalIF":0.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}