Investigating the Effect of Secretome Secreted from Adult Rat Kidney-Derived Stem Cells on the Expression of miR375, miR494 and Tissue Changes in Glycerol-Induced Acute Kidney Injury.

IF 0.7 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Advanced biomedical research Pub Date : 2025-05-31 eCollection Date: 2025-01-01 DOI:10.4103/abr.abr_338_24
Armita Valizadeh Gorji, Fereshtesadat Fakhredini, Fatemeh Soltanpour, Esrafil Mansouri
{"title":"Investigating the Effect of Secretome Secreted from Adult Rat Kidney-Derived Stem Cells on the Expression of miR375, miR494 and Tissue Changes in Glycerol-Induced Acute Kidney Injury.","authors":"Armita Valizadeh Gorji, Fereshtesadat Fakhredini, Fatemeh Soltanpour, Esrafil Mansouri","doi":"10.4103/abr.abr_338_24","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Acute kidney injury (AKI) causes high mortality. Cell therapy has provided acceptable results, but factors such as immune rejection and teratoma formation have limited their use. The purpose of this research is to study the reparative effects of secretome secreted from kidney-derived stem cells on glycerol-induced kidney damage in rats.</p><p><strong>Materials and methods: </strong>Secretomes were isolated from conditioned media of kidney mesenchymal stem cells. The animals were separated into 4 groups: Control, Secretome (received 30 μg/kg secretome, for 3 days), Glycerol (10 ml/kg of glycerol, intramuscularly) and Glycerol + Secretome (received glycerol and secretome). Blood urea (BUN), Creatinine (Cr) and Albumin (alb) levels were measured with detection kits. The histopathological results of the secretomes on kidney were checked with PAS staining and the amount of expression of miR-375 and miR-494 was assessed by quantitative real-time polymerase chain reaction (qRT-PCR).</p><p><strong>Results: </strong>The results of this research showed that intravenous of secretomes significantly decreased the levels of BUN (<i>P</i> < 0.01), Cr (<i>P</i> < 0.001), and alb (<i>P</i> < 0.001). Well as there was a considerable decreased in miR-375 (<i>P</i> < 0.001) and miR-494 (<i>P</i> < 0.01) in the receiving group of Glycerol + Secretome rats. The results showed secretomes improved kidney histopathology by inducing regulatory changes in the expression levels of microRNAs (miRNAs).</p><p><strong>Conclusion: </strong>The results of this research showed that secretomes extracted from kidney stem cells can play a role in reducing kidney damage by regulating miRNAs related to AKI.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"14 ","pages":"48"},"PeriodicalIF":0.7000,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165302/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced biomedical research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/abr.abr_338_24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Acute kidney injury (AKI) causes high mortality. Cell therapy has provided acceptable results, but factors such as immune rejection and teratoma formation have limited their use. The purpose of this research is to study the reparative effects of secretome secreted from kidney-derived stem cells on glycerol-induced kidney damage in rats.

Materials and methods: Secretomes were isolated from conditioned media of kidney mesenchymal stem cells. The animals were separated into 4 groups: Control, Secretome (received 30 μg/kg secretome, for 3 days), Glycerol (10 ml/kg of glycerol, intramuscularly) and Glycerol + Secretome (received glycerol and secretome). Blood urea (BUN), Creatinine (Cr) and Albumin (alb) levels were measured with detection kits. The histopathological results of the secretomes on kidney were checked with PAS staining and the amount of expression of miR-375 and miR-494 was assessed by quantitative real-time polymerase chain reaction (qRT-PCR).

Results: The results of this research showed that intravenous of secretomes significantly decreased the levels of BUN (P < 0.01), Cr (P < 0.001), and alb (P < 0.001). Well as there was a considerable decreased in miR-375 (P < 0.001) and miR-494 (P < 0.01) in the receiving group of Glycerol + Secretome rats. The results showed secretomes improved kidney histopathology by inducing regulatory changes in the expression levels of microRNAs (miRNAs).

Conclusion: The results of this research showed that secretomes extracted from kidney stem cells can play a role in reducing kidney damage by regulating miRNAs related to AKI.

研究成年大鼠肾源性干细胞分泌分泌组对甘油诱导急性肾损伤中miR375、miR494表达及组织变化的影响。
背景:急性肾损伤(AKI)死亡率高。细胞疗法提供了可接受的结果,但免疫排斥和畸胎瘤形成等因素限制了它们的使用。本研究旨在研究肾源性干细胞分泌的分泌组对甘油诱导大鼠肾损伤的修复作用。材料与方法:从肾间充质干细胞条件培养基中分离分泌组。将实验动物分为4组:对照组、分泌组(30 μg/kg)、甘油组(10 ml/kg,肌注)和甘油+分泌组(分别给予甘油和分泌组)。用检测试剂盒测定血尿素(BUN)、肌酐(Cr)、白蛋白(alb)水平。采用PAS染色检测肾脏分泌组的组织病理学结果,采用实时荧光定量聚合酶链反应(qRT-PCR)检测miR-375和miR-494的表达量。结果:本研究结果显示,静脉注射分泌组显著降低BUN (P < 0.01)、Cr (P < 0.001)、alb (P < 0.001)水平。甘油+分泌组大鼠miR-375 (P < 0.001)和miR-494 (P < 0.01)明显降低。结果显示,分泌组通过诱导microRNAs (miRNAs)表达水平的调节变化改善肾脏组织病理学。结论:本研究结果表明,从肾干细胞中提取的分泌组可通过调节AKI相关mirna,起到减轻肾损伤的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
0.90
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信