Biogenic Ag-Cu Nanoparticles Synthesized with Extract of Eryngium Billardieri L. and Evaluation of their Anticancer Potential on PC-3 and LNCaP Cancer Cell Lines.

IF 0.7 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Advanced biomedical research Pub Date : 2025-05-31 eCollection Date: 2025-01-01 DOI:10.4103/abr.abr_255_24
Zahra Ebrahim, Ghazaleh Feyzabadi, Nastaran Asghari Moghaddam, Azadeh Mohammadgholi
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引用次数: 0

Abstract

Background: The anticancer action of biogenic Ag-Cu nanoparticles (NPs) using Eryngium billardieri against PC-3 and LNCaP cell lines in prostate malignancy was investigated.

Materials and methods: XRD, FTIR, EDAX, FESEM, and TEM were used to illustrate the Ag-Cu NPs. The PC-3 and LNCaP cell lines were used to test the anticancer ability of Ag-Cu NPs. The cytotoxicity was assessed by MMT and flow cytometry. Apoptotic and metastatic-promoting gene expressions were evaluated by real-time PCR. ROS generation was measured.

Results: NPs are spherical and their average size was 12 ± 9.7 nanometers. The EDAX result indicated the presence of Ag, Cu, and C. The XRD results revealed the NPs' crystalline structure. The results demonstrated a remarkable antiproliferative effect of the nanoparticles, with IC50 values of 15.43 μg/mL for PC-3 and 7.64 μg/mL for LNCaP. The Ag-Cu NPs exhibited a tendency to trigger apoptosis. This was confirmed by analyzing apoptosis-related gene expression. The apoptotic influence of Ag-Cu NPs was suggested to be critical when compared to the control and extract-treated groups by the up-regulation of Bcl2-related X (Bax), caspase3, and caspase9, and the down-regulation of Bcl2. Furthermore, Annexin V-FITC/propidium iodide presented a 23.15% and 22.3% apoptotic ratio of PC-3 and LNCaP cells, respectively. Vascular endothelial growth factor (VEGF), and metalloproteinases (MMPs) down-regulation showed that Ag-Cu NPs were hostile to metastasis. Also, ROS generation was estimated at 1261 and 1366 RFU in LNCaP and PC-3 cells treated with green-synthesized Ag-Cu NPs, respectively.

Conclusions: The study suggested E. billardieri synthesized Ag-Cu as a potential candidate in prostate cancer therapeutic management.

枸杞提取物合成生物源银铜纳米颗粒及其对PC-3和LNCaP癌细胞的抗癌作用
背景:研究了生物源银铜纳米粒子(NPs)对前列腺恶性肿瘤PC-3和LNCaP细胞系的抗癌作用。材料和方法:采用XRD、FTIR、EDAX、FESEM和TEM对Ag-Cu NPs进行表征。用PC-3和LNCaP细胞株检测Ag-Cu NPs的抗癌能力。采用MMT和流式细胞术检测细胞毒性。实时荧光定量PCR检测促凋亡基因和促转移基因的表达。测定ROS的生成。结果:NPs呈球形,平均尺寸为12±9.7纳米。EDAX结果表明纳米粒子中存在Ag、Cu和c, XRD结果显示纳米粒子的晶体结构。结果表明,PC-3的IC50值为15.43 μg/mL, LNCaP的IC50值为7.64 μg/mL。Ag-Cu NPs有触发细胞凋亡的倾向。凋亡相关基因表达分析证实了这一点。通过上调Bcl2相关的X (Bax)、caspase3和caspase9,下调Bcl2,与对照组和提取物处理组相比,Ag-Cu NPs对细胞凋亡的影响至关重要。Annexin V-FITC/碘化丙啶对PC-3和LNCaP细胞的凋亡率分别为23.15%和22.3%。血管内皮生长因子(VEGF)和金属蛋白酶(MMPs)的下调表明Ag-Cu NPs对转移有不利影响。此外,用绿色合成的Ag-Cu NPs处理LNCaP和PC-3细胞的ROS生成估计分别为1261和1366 RFU。结论:本研究提示平络菌合成Ag-Cu是前列腺癌治疗的潜在候选药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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