Advanced biomedical research最新文献

{"title":"Guillain-Barré Syndrome (GBS) as a COVID-19 Complication: A Multicenter Study Conducted Throughout the 2-Year Pandemic.","authors":"Ansari Behnaz, Kheradmand Mohsen, Mohammad Amin Najafi, Basiri Keivan, Khorvash Fariborz, Forouzandeh Negin, Ghasemi Majid","doi":"10.4103/abr.abr_191_24","DOIUrl":"10.4103/abr.abr_191_24","url":null,"abstract":"<p><strong>Background: </strong>Several studies have been published on the coexistence of Guillain-Barré syndrome (GBS) and COVID-19, raising the question of whether SARS-CoV-2 plays a role in GBS pathogenesis or is just a coincidence. In this research, we discuss GBS characteristics in COVID-19 patients during the pandemic.</p><p><strong>Material and methods: </strong>This cross-sectional, multi-center study was performed on patients who were hospitalized in one of two GBS referral hospitals in Isfahan Providence between March 2020 to March 2022. All confirmed GBS and COVID-19 patients with no other known risk factor for GBS and no more than 28 days of COVID-19 treatment were entered into the trial.</p><p><strong>Results: </strong>Totally, 45 patients were collected. The most prevalent EDX pattern was AIDP (57.8%). As compared to demyelinating injuries, axonal injuries were significantly more associated with lung involvement (<i>P</i> value: 0.022), higher GBS disability score (<i>P</i> value: 0.034), poorer response to treatment (<i>P</i> value: 0.021), higher serum inflammatory level, and older onset (<i>P</i> value: 0.013). The average period between the onset of COVID-19 and the onset of GBS symptoms was 18.07 ± 9.88 days. 46.3% of patients developed GBS within the first two weeks of COVID-19, a condition known as a para-infection. The axonal injury was significantly more prevalent in these individuals (<i>P</i> value: 0.045).</p><p><strong>Conclusion: </strong>An earlier onset of neurological symptoms and para-infection conditions is associated with an increase in the frequency of axonal-type variations and, as a result, a poor prognosis. The majority of evidence in COVID-19-related GBS patients points to an autoimmune process.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"53"},"PeriodicalIF":1.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Role of <i>Trans</i>-chalcone against Kidney Injury Induced by High-Fat Diet: Exploring the Possible Involvement of FABP4 and Endoplasmic Reticulum Stress.","authors":"Elham Karimi-Sales, Khalil Hajiasgharzadeh, Parviz Shahabi, Mohammad Reza Alipour","doi":"10.4103/abr.abr_276_24","DOIUrl":"10.4103/abr.abr_276_24","url":null,"abstract":"<p><strong>Background: </strong>Obesity is a growing health concern, with an increasing prevalence. Overconsumption of a high-fat diet (HFD) is a risk factor for various diseases, including chronic kidney disease. Chronic HFD intake appears to be connected with ectopic deposition of lipids in the kidney, induction of endoplasmic reticulum (ER) stress, and renal dysfunction. <i>Trans-</i>chalcone is a flavonoid precursor with beneficial hepatoprotective, anti-inflammatory, anti-obesity, and anti-diabetic properties. Considering the role of fatty acid-binding protein 4 (FABP4) in triggering kidney diseases and inducing ER stress in response to lipids, the possible effects of <i>Trans</i>-chalcone and HFD intake on the renal expressions of FABP4 and indicators of ER stress were examined.</p><p><strong>Materials and methods: </strong>Twenty-one male Wistar rats were grouped into three groups: (1) control received 10% Tween 80, (2) HFD received a liquid HFD (10 mL/kg) and 10% Tween 80, and (3) HFD plus chalcone received a liquid HFD and <i>Trans</i>-chalcone (20 mg/kg). Administrations were performed by daily oral gavage for 42 consecutive days. Finally, fasting blood samples were used to evaluate Castelli's Risk Index-II (CRI-II). Additionally, the expression levels of FABP4, CAAT-enhancer-binding protein (C/EBP) homologous protein (CHOP), and glucose-regulated protein 78 (GRP78) in kidney samples were evaluated using real-time PCR.</p><p><strong>Results: </strong>Feeding with HFD significantly increased the atherogenic index (CRI-II) and the expression levels of FABP4, GRP78, and CHOP in the kidneys of animals. Conversely, <i>Trans</i>-chalcone significantly prevented these HFD-induced alterations.</p><p><strong>Conclusion: </strong><i>Trans</i>-chalcone can inhibit HFD-induced ER stress by suppressing FABP4 expression in the kidneys of rats fed the HFD.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"56"},"PeriodicalIF":1.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Difference Between Self-care and Body Awareness in Clients with and without Nephrolithiasis.","authors":"Fatemeh Tavakoli, Saeid Pournaghash Tehrani, Gholam Ali Afrooz, Iman Ghanaat, Hamid Kazemi-Zahrani","doi":"10.4103/abr.abr_369_24","DOIUrl":"10.4103/abr.abr_369_24","url":null,"abstract":"<p><strong>Background: </strong>The aim of the present study was to examine the differences between self-care and body awareness in individuals with and without nephrolithiasis.</p><p><strong>Materials and methods: </strong>This descriptive research was conducted among individuals with and without nephrolithiasis in Isfahan city. The sample size for the study was estimated to be 384 individuals for both the nephrolithiasis group and the non-nephrolithiasis group, based on the formula for an unlimited population. Targeted sampling was used, selecting individuals who met the inclusion criteria. Participants were interviewed regarding the inclusion criteria, and those who met the criteria completed the research questionnaires. The data collection tools used in this study were the Self-Care Ability Scale and the Body Awareness Questionnaire. The obtained data were analyzed using SPSS-24 and AMOS-24 software through ANOVA and multivariate analysis of variance.</p><p><strong>Results: </strong>The results showed that there is a difference in self-care and body awareness between individuals with and without nephrolithiasis, with the magnitude of this difference being 0.48 and 0.30, respectively.</p><p><strong>Conclusion: </strong>Based on the results, it can be concluded that there is a difference in self-care scores and body awareness between individuals with and without nephrolithiasis. Self-care measures and body awareness are higher and better in individuals without nephrolithiasis compared to those with nephrolithiasis.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"60"},"PeriodicalIF":1.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RE: The Level of Serum Osmolarity at Admission in Prognosis of Nosocomial Mortality in Patients with Severe Brain Trauma.","authors":"Mehdi Nematbakhsh","doi":"10.4103/abr.abr_475_24","DOIUrl":"10.4103/abr.abr_475_24","url":null,"abstract":"","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"63"},"PeriodicalIF":1.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barbaloin Attenuates Cerebral Ischemia and Reperfusion Injury in Rats and Inhibits Lipopolysaccharide-Stimulated Inflammatory Responses in BV2 Microglial Cells.","authors":"Caiyun Liu, Lin Wang, Hongyan Ren, Lijun Liu, Zhihu Yang","doi":"10.4103/abr.abr_236_24","DOIUrl":"10.4103/abr.abr_236_24","url":null,"abstract":"<p><strong>Background: </strong>Barbaloin (BAR) is a naturally occurring bioactive anthracycline derived from the leaf exudates of aloe plants with a variety of biological and pharmacological properties. The aim of the present study was to investigate the neuroprotective effects of BAR against cerebral ischemia and reperfusion (I/R) injury in rats and the possible underlying mechanisms.</p><p><strong>Materials and methods: </strong>Middle cerebral artery occlusion followed by reperfusion was used to induce cerebral I/R injury in rats, and BAR was administered intraperitoneally after the onset of ischemia.</p><p><strong>Results: </strong>We found that BAR remarkably improved neurological scores, reduced brain infarct volume, and inhibited neuronal apoptosis in cerebral I/R rats. Furthermore, BAR upregulated Bcl-2 protein levels and downregulated cleaved caspase-3, Bax, and inducible nitric oxide synthase (iNOS) in the ischemic cortex. I/R injury-induced increases in malondialdehyde content, and decreases in glutathione, glutathione peroxidase, and superoxide dismutase activities were significantly attenuated by BAR administration. <i>In vitro</i>, BAR pretreatment inhibited the release of proinflammatory cytokines (iNOS, tumor necrosis factor-α, and interleukin-6) and reduced protein levels of iNOS and nuclear expression of nuclear factor-κB p65 in lipopolysaccharide-stimulated BV-2 microglial cells.</p><p><strong>Conclusion: </strong>Taken together, our data suggest that BAR possesses neuroprotective effects against cerebral I/R injury through antiapoptotic, antioxidative, and anti-inflammatory properties.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"55"},"PeriodicalIF":1.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Antifungal Activity of Metabolites Produced by Soil Actinomycetes on the <i>Candida</i> Species Isolated from Candidemia.","authors":"Raheleh Mostafavi Yousofabad, Mehrnoush Maheronnaghsh, Zeinab Moussaie, Parvin Dehghan","doi":"10.4103/abr.abr_403_24","DOIUrl":"10.4103/abr.abr_403_24","url":null,"abstract":"<p><strong>Background: </strong>Actinomycetes are a well-known group of bacteria that produce critical metabolites. This study aimed to identify the bioactive actinomycetes from soil samples that exhibit antifungal activity against <i>Candida</i> species isolated from the blood of patients with candidemia.</p><p><strong>Materials and methods: </strong>Twelve soil samples from southwestern Iran were cultured on starch casein agar to isolate actinomycetes. The antifungal activity of the isolates was screened using a modified overlay agar technique. Five <i>Candida</i> species isolated from candidemia patients (<i>Candida albicans, C.glabrata, C.lusitania, C. tropicalis</i>, and <i>C. parapsilosis</i>) were tested using well diffusion agar assays. Seven actinomycetes with the strongest anticandidal activity were identified by 16S rDNA gene sequencing and compared to gene sequences in the NCBI database using the BLAST program.</p><p><strong>Results: </strong>Of the 52 actinomycete isolates, seven demonstrated significant antifungal potency against <i>Candida</i> isolates. Sequencing identified four actinomycete genera: <i>Crossiell</i>a, <i>Streptomyces, Saccharothrix</i>, and <i>Amycolatopsis</i>. <i>Crossiella cryophila</i> showed the strongest inhibition against <i>Candida parapsilosis</i>, with a halo diameter of 42.5 ± 13 mm. <i>Streptomyces</i> exhibited a weaker inhibitory effect across all tested <i>Candida</i> species, with its highest activity against <i>C. parapsilosis</i> (a halo diameter of 35 ± 17.3 mm, <i>P</i> ≤ 0.05). <i>Amycolatopsis alba</i> displayed the weakest antifungal effect against <i>C. lusitaniae</i> compared to other <i>Candida</i> species (<i>P</i> ≤ 0.05).</p><p><strong>Conclusions: </strong>This study reports the presence of novel actinomycetes in Iranian soil that can produce anti-<i>Candida</i> compounds. These isolates hold promise for potential therapeutic applications in treating patients with candidemia.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"59"},"PeriodicalIF":1.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in Immunological and Inflammatory Biomarkers Levels in People Infected with <i>Ascaris lumbricoides</i> according to Different Age Groups.","authors":"Zainab Naser Al-Laith, Aseel Kariem Al-Sultany, Damiaa I Aljubory, Osama A Mohsein","doi":"10.4103/abr.abr_172_24","DOIUrl":"10.4103/abr.abr_172_24","url":null,"abstract":"<p><strong>Background: </strong>Prevalent parasite infection, <i>Ascaris lumbricoides</i> affects millions of people globally, especially in inadequate sanitation and hygiene. Swallowing <i>A</i>. <i>lumbricoides</i> eggs from contaminated food, water, or soil causes infection. This study examines age-related differences in interleukin levels and immunological response.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, the samples were collected from <i>A</i>. <i>lumbricoides</i>-infected persons diagnosed by a general stool examination and divided into six age groups. The study comprised 180 samples from Al-Habbobi and Al-Nassiriya teaching hospitals and private clinics across six age groups. The control group had 90 worm-free samples. Forty samples were removed for not meeting the research criteria. The study assessed C-reactive protein (CRP), IL4, IL5, and TNF-a by ELISA and WBC count by Sysmex CBC. Anova, Mann-Whitney, and <i>t</i>-tests showed biological indicator differences between groups.</p><p><strong>Results: </strong>Significant increases in CRP, WBC, Interleukin-4, and Interleukin-5 were observed in all study groups across 6 age groups compared to the control group (<i>P</i> ≤ 0.01). TNF levels decreased significantly in the first group compared to the control group (<i>P</i> ≤ 0.01), but they increased significantly in the other groups (<i>P</i> ≤ 0.01).</p><p><strong>Conclusions: </strong>The high number of white blood cells indicates the presence of inflammation. In addition, high CRP levels indicate the severity of the infection caused by the parasite. Because of the immune response carried out by the immune system as a means of defense against the parasite, this leads to high levels of interleukin-4, interleukin-5 and TNF-alpha.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"43"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Efficacy of the Administration of <i>Bifidobacterium lactis</i> for Treatment of Infantile Colic: A Double-Blind, Randomized, Placebo-Controlled Clinical Trial.","authors":"Mohammad Ali Pourmirzaiee, Fatemeh Famouri, Akram Kahied, Silva Hovsepeian, Roya Kalishadi","doi":"10.4103/abr.abr_185_24","DOIUrl":"10.4103/abr.abr_185_24","url":null,"abstract":"<p><strong>Background: </strong>Although there is evidence regarding the effectiveness of probiotics for infantile colic (IC), there are not many studies regarding the efficacy of Bifidobacterium animalis subsp. Lactis BB-12^®, (BB-12^®), for the treatment of IC. This trial aimed to evaluate the efficacy of this subspecies of probiotic administration on IC.</p><p><strong>Materials and methods: </strong>In this double-blind randomized, placebo-controlled clinical trial, infants with IC diagnosis, exclusive breastfeeding, gestational age of more than 37 weeks, and birth weight of more than 2500 grams were included. The selected infants were randomly allocated into the two groups of \"BBcare\" group, treated with BB-12^® and the control group was treated with a placebo, 5 drops per day, for 6 weeks. The mean of crying time, vomiting episodes, and frequency of defecation at baseline and during follow-ups (40 and 60 days after intervention) were compared between the two studied groups.</p><p><strong>Results: </strong>In this study, 40 and 38 neonates in \"BBcare\" and placebo groups completed the study. In the \"BBcare\" group frequency of defecation, crying time, and vomiting episodes decreased significantly during intervention till 60 days after probiotic administration (<i>P</i> < 0.05). In the placebo group, there was a significant increase in crying and vomiting episodes between baseline and 60 days after intervention (<i>P</i> < 0.05). Between-group analyses indicated that mean of crying time, and vomiting episodes and number of defecations, 60 days after intervention were significantly lower in \"BBcare\" group (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>The findings of our study offer compelling signals for the effectiveness of BB-12^® in the management of some intestinal problems. Although these findings could be supportive evidence for the important role of gut microbiota as the goal of intervention to improve bowel movement and comfortable defecation in IC, further studies are needed in this field, especially for the evaluation of different types of probiotics in combination with each other's and with different dosages.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"47"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Moderate-Intensity Training and MgSo₄ Against Lithium-Induced Renal and Oxidative Damages in Rat Kidney.","authors":"Elham Shoghi, Saideh Miri, Zahra Nekhat, Abbas Ali Niazi, Rana Hashemi, Parsa Niyazi, Hossein Nakhaie, Hossein Bagheri, Mohamad Mahdi Vahedi, Elham Shahraki, Meysam Zeinali Bojani, Fatemeh Maghool, Tahereh Safari","doi":"10.4103/abr.abr_89_24","DOIUrl":"10.4103/abr.abr_89_24","url":null,"abstract":"<p><strong>Background: </strong>Lithium (Li), as the first line of treatment in bipolar disorder, causes damage to many biological systems, including the kidneys, through the induction of oxidative stress. Magnesium sulfate (MgSo₄) and moderate-intensity training (MIT) can diminish the injury caused by Li due to reducing oxidative stress. In this study, we explore the effects of these two factors on Li-induced renal damage.</p><p><strong>Materials and methods: </strong>Seventy-two male rats were divided into 12 groups: control, MgSo₄ 80 mg/kg/day/ip, MIT, MgSo₄ + MIT, Li10 mg/kg/day/ip, Li10 + MgSo₄, Li10 + MIT, Li10 + MgSo₄ + MIT, Li40 mg/kg/day/ip, Li40 + MgSo₄, Li40 + MIT, and Li40 + MgSo₄ + MIT. All animals were under the exercise protocol for 6 weeks and received the drugs daily. A blood sample was obtained from the heart to measure blood urea of nitrogen (BUN) and creatinine (Cr). The right kidney was excised and homogenized to measure malondialdehyde (MDA), nitrite, total antioxidant capacity (TAC), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and the left kidneys were placed in 10% formalin to be referred to the laboratory for pathological investigations.</p><p><strong>Results: </strong>A significant increase in the serum levels of BUN and Cr in the Li40 mg/kg group in comparison with the control group indicates kidney tissue damage, which improved after administration of MgSo₄ and MIT. Treatment with Li10 mg/kg and Li40 mg/kg decreased the TAC, SOD, and GPx and increased the MDA in kidney tissue significantly, which improved after administration of MgSo₄ and MIT in comparison with the Li10 and Li40 groups.</p><p><strong>Conclusion: </strong>The findings of this study indicated that exercise and MgSo₄ reduced the nephrotoxicity caused by Li via reducing oxidative stress.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"44"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homing Assessment of Human Placenta-Derived Mesenchymal Stromal Cells in Immunodeficient NOG Mice Following Sublethal Irradiation.","authors":"Nasser Ahmadbeigi, Samad Muhammadnejad, Ahad Muhammadnejad, Mohammad Vasei, Kobra Moradzadeh, Javad Verdi, Maria Kavianpour","doi":"10.4103/abr.abr_536_23","DOIUrl":"10.4103/abr.abr_536_23","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal stromal cells (MSCs) are among the most extensively studied candidates for cell-based therapy. The homing capacity of MSCs is a critical factor influencing the efficacy of MSC-based therapies. This study aimed to investigate the homing of placenta-derived mesenchymal stromal cells (PD-MSCs) in various damaged tissues of immunodeficient Cg-PrkdcscidIl2rgtm1Sug/ShiJic (NOG) mice in response to total body irradiation (TBI).</p><p><strong>Materials and methods: </strong>NOG mice were irradiated with 3.5 Gy. PD-MSCs were isolated, characterized, and transduced with green fluorescent protein (GFP). Twenty-four hours after irradiation, 250 × 10³ GFP-PD-MSCs were injected intravenously through the tail vein in the intervention group, while the control group received phosphate-buffered saline. Peripheral blood, bone marrow, spleen, lungs, liver, intestine, and kidneys were collected at two time points (2 and 14 days postexposure). Samples were analyzed using flow cytometry, polymerase chain reaction (PCR), and immunohistochemistry (IHC).</p><p><strong>Results: </strong>A weak band was detected in some PCR samples following GFP-PD-MSCs injection; however, the presence of these cells was not confirmed by flow cytometry or IHC.</p><p><strong>Conclusion: </strong>Intravenous infusion of GFP-PD-MSCs did not result in detectable homing to various damaged tissues in response to TBI. This suggests that the inability of these cells to home effectively may limit their therapeutic potential.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"41"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}