Advanced biomedical research最新文献_第3页

Evaluation of Diagnostic Value of Frozen Section for Ovarian Masses in a Referral Hospital in Central Part of Iran, with Focus on Borderline Cases. 伊朗中部一家转诊医院卵巢肿块冷冻切片诊断价值评价，以边缘性病例为重点。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_274_23

Azar Naimi, Reihaneh Banihashemi

{"title":"Evaluation of Diagnostic Value of Frozen Section for Ovarian Masses in a Referral Hospital in Central Part of Iran, with Focus on Borderline Cases.","authors":"Azar Naimi, Reihaneh Banihashemi","doi":"10.4103/abr.abr_274_23","DOIUrl":"10.4103/abr.abr_274_23","url":null,"abstract":"Background: Differentiating between benign ovarian masses and malignant ones is done using a variety of pathological examination methods. The two most prominent are frozen section diagnosis and permanent pathology diagnosis, the gold standard. We conducted this study with the aim of assessing the accuracy of frozen section diagnosis in ovarian masses.Materials and methods: We studied different cases of ovarian masses with pathologic studies done using frozen section diagnosis and then compared the results with the final diagnosis using permanent studies. Sensitivity, specificity, positive predictive value, and negative predictive value were then calculated. Afterwards, borderline cases were grouped separately, and the aforementioned values were calculated for both borderline cases in isolation and overall cases.Results: In this study, 350 cases were included. The women from whom the samples were taken had an average age of 44.44 ± 15.15. Frozen section diagnosis underperforms in borderline cases, usually underdiagnosing them. In this study, we found the overall sensitivity = 82.3%, specificity = 98.9%, positive predictive value = 94.4%, and negative predictive value = 96.3%. However, if the borderline cases were excluded, they change to sensitivity = 82.2%, specificity = 99.3%, positive predictive value = 95.3%, and negative predictive value = 96.9%.Conclusion: Frozen section diagnosis is an accurate and reliable method that can rule out almost every benign ovarian mass. While it is notably weaker in the diagnosis of malignant and borderline tumors, its value as a diagnosis tool cannot be denied.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"37"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Holistic Bibliometric Exploration of the Evolution of Epidermodysplasia Verruciformis (EV) Research. 疣状表皮发育不良（EV）研究演变的整体文献计量学探索。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_386_25

Farshid Danesh, Fatemeh Alinezhad Chamazcoti, Forough Rahimi, Saeed Bakhtiari

{"title":"A Holistic Bibliometric Exploration of the Evolution of Epidermodysplasia Verruciformis (EV) Research.","authors":"Farshid Danesh, Fatemeh Alinezhad Chamazcoti, Forough Rahimi, Saeed Bakhtiari","doi":"10.4103/abr.abr_386_25","DOIUrl":"10.4103/abr.abr_386_25","url":null,"abstract":"Background: Epidermodysplasia Verruciformis (EV) is a rare autosomal recessive genodermatosis caused by chronic HPV infection. It leads to widespread cutaneous lesions and an increased risk of non-melanoma skin cancer. The main objective of this article is to conduct a holistic bibliometrics analysis of scientific publications, uncovering trends and citation networks related to EV (1931-2025).Materials and methods: Data were extracted from the Web of Science Core Collection (1931-2025), and 1489 documents were retrieved. Bibliometric methods and related indicators, including country collaboration map, word co-occurrence, co-citation networks, topics, citations, and publications trends, were analyzed and visualized using the Bibliometrix package in R.Results: The document collection comprises 99 single-authored publications, with a Document Average Age (DAA) of 20.2 years (i.e., the average time elapsed between publication and the year of analysis, 2025), 5.36 authors per document, 32.15 citations per document, and a 2.09% annual growth rate. The five most frequent thematic trends are EV, deoxyri bonucleic acid (DNA), Squamous Cell Carcinoma, Renal Transplant Recipients, and Infection, with frequencies of 423, 167, 127, 121, and 100. The United States, Germany, and Great Britain have the highest numbers of citations, with 13,998, 6,476, and 4,776. \"G. Orth,\" \"S. Jablonska,\" and \"M. Favre\" received the highest number of citations in EV. The most influential journals are \"Journal of Virology,\" \"Journal of Investigative Dermatology,\" and \"Virology\".Conclusion: Epidemiological studies on EV prevalence and high-risk human papillomavirus (HPV) types are crucial for policymakers and health planners. Interdisciplinary research, using artificial intelligence, image processing, and machine learning, can enhance research and collaboration.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"48"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Medication Never Events and Related Factors: A Nationwide Database Study. 用药不良事件及相关因素的评价：一项全国性数据库研究。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_484_23

Mehdi Nasr Isfahani, Behrooz Keleidari, Parvaneh Mahmoodi, Amin Salehi, Asieh Maghami Mehr

{"title":"Evaluation of the Medication Never Events and Related Factors: A Nationwide Database Study.","authors":"Mehdi Nasr Isfahani, Behrooz Keleidari, Parvaneh Mahmoodi, Amin Salehi, Asieh Maghami Mehr","doi":"10.4103/abr.abr_484_23","DOIUrl":"10.4103/abr.abr_484_23","url":null,"abstract":"Background: The present study was conducted to evaluate the prevalence of medication errors regarded as never events in Iran and the related factors and develop a strategic operation plan (SOP) for its prevention.Materials and methods: A nationwide cross-sectional study with a documentary approach was conducted. For this purpose, all documented reports of medication-never events (over 515 cases) were extracted from the hospital Information system (HIS) of Iran between 2018 and 2020. After reporting each error, using root cause analysis (RCA), factors related to each medication never event were identified.Results: According to the evaluations, the errors caused by the name/type of medication and medication administration errors had the highest percentages, 38.4% and 25%, respectively. 14.8% of patients were infants, 13.4% children, 4.3% youth, 43.7% adults, and 23.9% were elderly. Among healthcare providers (HCP), nurses were individuals who committed these errors the most (72.2%). Analyzing the causes and factors related to error incidence, the negligence of health-care personnel, the negligence of the patient or his companion, work environment problems, name problems, packaging and drug labels, negligence in responsibilities, problems of computer information systems, and handwritten prescriptions were found to be the most common factors related to the occurrence of medication never events.Conclusion: The results revealed that strategies contributing to the minimization of each type of error may include education of the patient or healthcare provider. By implementing a computer-based system for medication review and reconciliation process, prioritizing areas that can be quickly modified, might be more feasible.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"40"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Well-Tolerated Intracorneal Wood Foreign Body of 25-Year Duration. 25年角膜内木质异物耐受性良好。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_254_22

Seyed Ali Sonbolestan, Zahra-Alsadat Abtahi

引用次数: 0

Comparison of One-Stage (Posterior-Only Approach) and Two-Stage (Combined Anterior-Posterior Approach) Surgery in Treating Spinal Tuberculosis. 一期（单纯后路）与二期（前后联合入路）手术治疗脊柱结核的比较。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_546_24

Ali Andalib, Majid Rezvani, Soroush Kazerani

{"title":"Comparison of One-Stage (Posterior-Only Approach) and Two-Stage (Combined Anterior-Posterior Approach) Surgery in Treating Spinal Tuberculosis.","authors":"Ali Andalib, Majid Rezvani, Soroush Kazerani","doi":"10.4103/abr.abr_546_24","DOIUrl":"10.4103/abr.abr_546_24","url":null,"abstract":"Background: Limited research has examined the effectiveness of posterior-only versus combined anterior-posterior surgical techniques for spinal tuberculosis (STB). This study sought to assess and compare these two surgical methods.Materials and methods: We conducted a prospective review of 20 patients with STB who received a posterior-only intervention (group A) and 20 patients who underwent a combined anterior-posterior procedure (group B). We analyzed and compared the outcomes of both groups.Results: Both groups showed significant improvements in the visual analogue scale and Oswestry Disability Index; however, there were no significant differences between the two groups. The average hospital stay for group A was significantly shorter (P ≤ 0.05). No significant differences were observed in any other measured outcomes between the two groups. One patient from group A and two patients from group B experienced infections at the surgical site, and one individual in each group developed pseudoarthrosis.Conclusion: Both the combined anterior-posterior and posterior-only surgical approaches can yield satisfactory clinical and radiological results in treating STB. Conversely, the posterior-only approach effectively achieves lesion debridement, decompression, and the maintenance of stability, along with the potential benefit of a reduced hospital stay.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"45"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Dosimetric Analysis of Jaw Tracking Versus Non-Jaw Tracking Techniques in Head and Neck and Brain Cancers: A Systematic Review and Meta-Analysis. 头颈部和脑癌中下颌追踪与非下颌追踪技术的比较剂量学分析：一项系统综述和荟萃分析。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_420_23

Javad Garshad, Baranoosh Rahmani, Mahsa Mansourian

{"title":"Comparative Dosimetric Analysis of Jaw Tracking Versus Non-Jaw Tracking Techniques in Head and Neck and Brain Cancers: A Systematic Review and Meta-Analysis.","authors":"Javad Garshad, Baranoosh Rahmani, Mahsa Mansourian","doi":"10.4103/abr.abr_420_23","DOIUrl":"10.4103/abr.abr_420_23","url":null,"abstract":"Cancers affecting the head, neck, and brain are highly prevalent and pose a significant threat to human society. Radiation therapy is the primary treatment modality for these types of tumors, including intensity-modulated radiotherapy (IMRT) and volumetric-modulated arc therapy (VMAT) being the prominent methods. However, concerns regarding the protection of organs at risk (OARs) persist when using these techniques. To address this, the use of the jaw tracking technique in conjunction with IMRT and VMAT, along with minimizing radiation leakage between leaves, is proposed as a protective measure. Our study aimed to comprehensively examine the quantitative and qualitative impact of jaw tracking on dose reduction in the normal tissues of patients with head, neck, and brain cancers. A systematic literature search was conducted via PubMed, Scopus, and Web of Science up to May 2023, resulting in the identification of 674 relevant studies. After applying our criteria, only 5 studies met the inclusion criteria. Our findings indicate that the jaw tracking technique can effectively determine the dose reaching the OAR. However, further research with a larger sample size is necessary to validate and explore further base on these results.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"39"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Pivotal Role of miR-770 in Solid Tumors Carcinogenesis. miR-770在实体瘤癌变中的关键作用。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_307_23

Mohsen Davari, Pooran Ghiasizadeh, Mozhgan Mondeali, Mohammadreza Rajabi, Forough Hatami, Sara Zareian

{"title":"The Pivotal Role of miR-770 in Solid Tumors Carcinogenesis.","authors":"Mohsen Davari, Pooran Ghiasizadeh, Mozhgan Mondeali, Mohammadreza Rajabi, Forough Hatami, Sara Zareian","doi":"10.4103/abr.abr_307_23","DOIUrl":"10.4103/abr.abr_307_23","url":null,"abstract":"MicroRNAs (miRNAs) are known as potential biomarkers for cancer detection and therapeutic management owing to their epigenetic significance in gene expression and other cellular functions. In this review, evidence has been collected to reach the clinical effect and molecular mechanism of miR-770 in various cancers. MiR-770 dysregulation in different malignancies like breast cancer, colorectal cancer, gastric cancer, glioma, liver cancer, lung cancer, oral cancer, prostate cancer, and ovarian cancer has shown a significant decrease or increase in its expression, which indicates it acts as a tumor suppressor or oncogene. MiR-770 participates in cell growth, migration, and invasion via the cellular pathway, WNT (wingless-type MMTV integration site family)/β-catenin signaling pathways, Toll-like receptor signaling pathway, mitogen-activated protein kinases (MAPK) signaling pathway, phosphatidylinositol 3kinase (PI3K)-protein kinase B (AKT) signaling pathway, Notch signaling pathway, erythroblastic leukemia viral oncogene homolog (ErbB) signaling pathway, transforming growth factor-beta (TGF-beta) signaling pathway, Insulin signaling pathway, ubiquitin-dependent proteolysis, Neurotrophin signaling pathway, vascular endothelial growth factor (VEGF) signaling pathway, and T cell and B cell receptor signaling pathway. In the present review, MiR-770 has been introduced as a potential marker in the prognosis of various cancers as well as in the early identification of tumor formation, progression, and metastasis.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"38"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Meta-Analysis: Unveiling the Link between Apolipoprotein E and Diabetic Retinopathy. 一项综合荟萃分析：揭示载脂蛋白E与糖尿病视网膜病变之间的联系。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_155_24

Pooja Bhardwaj, Swati Shukla, Sana Nafees, K M Ambika, Manzoor Ahmad Malik, Vinod Kumar, Jasbir Kaur

{"title":"A Comprehensive Meta-Analysis: Unveiling the Link between Apolipoprotein E and Diabetic Retinopathy.","authors":"Pooja Bhardwaj, Swati Shukla, Sana Nafees, K M Ambika, Manzoor Ahmad Malik, Vinod Kumar, Jasbir Kaur","doi":"10.4103/abr.abr_155_24","DOIUrl":"10.4103/abr.abr_155_24","url":null,"abstract":"Diabetic retinopathy (DR), a major diabetic microvascular complication, is a leading cause of visual loss. DR is often insidious and asymptomatic at the early stages. There has been a worldwide effort in the identification of key marker(s) that detect the disease before its onset. Apolipoprotein E (ApoE) gene has been suggested to be a potential risk factor for the development of micro- and macrovascular complications in diabetes. There are a few published reports available on the relationship between ApoE and DR; however, the outcome of these studies is inconsistent. Till date, there is no meta-analysis available on the association between ApoE and DR. Therefore, we have performed meta-analysis to achieve a more accurate conclusion regarding the relationship between ApoE genetic polymorphism and DR. Case-control studies analyzing ApoE polymorphism and DR were selected from databases, including PubMed and Google, as per the inclusion and exclusion criteria. Eligible data were extracted, pooled, analyzed, and evaluated using Comprehensive Meta-Analysis version-4. A total of five case-control studies were included, comprising 1,572 cases and 2,746 controls. The genotypes of ApoE were compared between cases and controls. Two genotypes, ɛ2ɛ2 and ɛ2ɛ3, were compared with ɛ2ɛ4 and ɛ3ɛ3. According to the analysis, there was no significant association between ApoE genotypic polymorphisms and DR (OR = 1.008, 95% CI = 0.702 -1.447, P = 0.967). In this meta-analysis, we found that ApoE polymorphisms were not associated with DR, suggesting that ApoE gene polymorphism does not determine genetic susceptibility of DR. Other genetic and environmental factors may be involved in DR development.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"46"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Different Domains of Perceived Stress in the Development of Cardiovascular Events: A Cohort Study. 不同感知压力域在心血管事件发展中的作用：一项队列研究。

IF 1.1

Advanced biomedical research Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_266_23

Hamidreza Roohafza, Farshad Rostampour, Ali Safaei, Fatemeh Nouri, Mohammad Talaei, Mansoureh Boshtam, Nizal Sarrafzadegan, Masoumeh Sadeghi

{"title":"The Role of Different Domains of Perceived Stress in the Development of Cardiovascular Events: A Cohort Study.","authors":"Hamidreza Roohafza, Farshad Rostampour, Ali Safaei, Fatemeh Nouri, Mohammad Talaei, Mansoureh Boshtam, Nizal Sarrafzadegan, Masoumeh Sadeghi","doi":"10.4103/abr.abr_266_23","DOIUrl":"10.4103/abr.abr_266_23","url":null,"abstract":"Background: Long-term distress plays a crucial role in the development and prognosis of cardiovascular events (CVEs). The current study aims to evaluate the role of distress and its domains in the pathogenesis of CVE.Materials and methods: This study is part of the Isfahan Cohort Study conducted on 3283 healthy individuals in 2007-2015 to assess the distress level, domains of perceived stressors, and their correlation with CVE development. The demographic and lifestyle characteristics of the studied population were recorded. General Health Questionnaire-12 (GHQ-12) and the Stressful Life Event questionnaire (SLE) were applied to investigate the stress level and its domains, respectively. Kaplan-Meier plots were depicted to show the correlation of CVE incidence with both stress statuses at study entry and the perceived stress level within the 8-year follow-up.Results: The logistic regression assessments showed that the stress score was an independent predictor for CVEs incidence in both crude model (P value = 0.001; OR: 1.08; 95%CI: 1.03-1.13) and by controlling the sociodemographic (P value = 0.004; OR: 1.08; 95%CI: 1.02-1.12) and lifestyle factors (P value = 0.001; OR: 1.07; 95%CI: 1.01-1.13). Domains, including home life, financial problems, personal conflicts, loss and separation, and health concerns, were independently associated with CVEs incidence regardless of sociodemographic and lifestyle factors (P value < 0.05).Conclusions: The current study represented an independent role for long-term distress in the development of CVEs. Besides, home life, financial problems, personal conflicts, loss and separation, and health concerns were the major long-term perceived stressors responsible for CVE development.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"42"},"PeriodicalIF":1.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protecting Effects of Rutin and N-Acetyl Cysteine on PON1 Enzyme in Cyclosporine A-Treated Rats: An In-Vivo, In-Vitro, and In-Silico Study. 芦丁和n-乙酰半胱氨酸对环孢素a治疗大鼠PON1酶的保护作用：体内、体外和计算机研究

IF 1.1

Advanced biomedical research Pub Date : 2026-05-29 eCollection Date: 2026-01-01 DOI: 10.4103/abr.abr_670_25

Sana Banisakin, Tooba Abdizadeh, Esfandiar Heidarian, Javad Saffari-Chaleshtori

{"title":"Protecting Effects of Rutin and N-Acetyl Cysteine on PON1 Enzyme in Cyclosporine A-Treated Rats: An In-Vivo, In-Vitro, and In-Silico Study.","authors":"Sana Banisakin, Tooba Abdizadeh, Esfandiar Heidarian, Javad Saffari-Chaleshtori","doi":"10.4103/abr.abr_670_25","DOIUrl":"10.4103/abr.abr_670_25","url":null,"abstract":"Background: Paraoxonase 1 (PON1) is a potent antioxidant enzyme in serum that protects against the oxidation of low-density lipoprotein. This study investigated the effect of N-acetylcysteine (NAC), a synthetic precursor to the natural antioxidant glutathione, and Rutin, a natural antioxidant, on protecting PON1 enzyme activity in rats treated with cyclosporine A (CsA) using experimental and molecular dynamics simulation studies.Materials and methods: In this study, 48 male rats were divided into six groups, each consisting of eight rats. Groups I, II, and III were CsA-free, while groups IV, V, and VI received 25 mg/kg CsA. Groups V and VI received daily NAC and Rutin (100 mg/kg) via intragastric gavage. After 21 days of treatment, all rats were euthanized, and the high-density lipoprotein parameter was measured using an auto analyzer in serum samples. PON1 enzyme activity was determined by its arylesterase property in serum samples. Docking and molecular dynamics simulation studies were performed using AutoDock V.4.2 and GROMACS 2022 software.Results: CsA decreased the PON1 enzyme arylesterase activity, while this activity increased in the groups that received CsA and Rutin, and/or CsA and NAC. The simulation results showed that all three compounds could bind into the PON1 independently and moderate its activity. Though serum PON1 arylesterase activity decreases in CsA received group, the combination of NAC or Rutin has a synergistic effect in further boosting PON1 aryl esterase enzyme activity when CsA is administered.Conclusion: The present findings indicate that NAC and Rutin have protective effects on the PON1 enzyme in CsA-treated adult rats.","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"35"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0