Advanced biomedical research最新文献

{"title":"Standard Methods for Quality Control in Cell-Based Medicinal Products and their Validation for Clinical Applications.","authors":"Elahe Mahmoodi Khaledi, Azam Samadian, Narges Sabahi Moosavi, Sara Dashtbozorgi, Simin Foroughian, Elham Rismani, Ensiyeh Hajizadeh-Saffar, Massoud Vosough","doi":"10.4103/abr.abr_269_25","DOIUrl":"10.4103/abr.abr_269_25","url":null,"abstract":"<p><p>In recent years, cell-based medicinal products (CMPs) have emerged as novel therapeutics with specific potential to treat a wide range of diseases. These products should be produced in accordance with good manufacturing practices (GMPs) and follow specific guidelines to ensure their safety and meet standard quality control criteria. This manuscript reviews current standard methods for quality control and validation specific to CMPs intended for clinical applications. We summarize critical quality attributes, including safety assessments such as sterility, endotoxin, mycoplasma, viral testing, tumorigenicity, and genetic stability; quantitative parameters, including cell counts and dose determination; and quality characteristics encompassing cell viability, morphology, growth kinetics, and immunophenotyping. We also address purity evaluation, potency assays, and the importance of validating analytical methods to guarantee reproducible and reliable test results. Furthermore, this article discusses necessary considerations for donor screening, raw material sourcing, manufacturing environment monitoring, and stability testing to maintain product integrity throughout production and storage. Emphasis is placed on adherence to GMP and relevant regulatory guidelines as defined by international pharmacopeias and authorities, such as the Food and Drug Administration and the European Medicines Agency. Finally, we highlight challenges faced in standardizing quality control for CMPs and underscore the need for continued development of rapid and robust testing methods tailored to their unique characteristics. This comprehensive overview aims to support academic and industrial stakeholders in implementing effective quality control strategies for advanced cell-based therapies.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"34"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling of Factors Affecting Hospital Length of Stay in ST-Elevation Patients Using Logistic Regression Models: SEMI CI Study.","authors":"Mohammad Agharazi, Ehsan Shirvani, Marjan Jamalian, Hamidreza Roohafza, Parsa Tavassoli Naini, Azin Karimian, Safoura Yazdekhsati, Ali Riahi, Masoumeh Sadeghi","doi":"10.4103/abr.abr_50_25","DOIUrl":"10.4103/abr.abr_50_25","url":null,"abstract":"<p><strong>Background: </strong>Ischemic heart disease is a prevalent condition. The length of stay (LOS) for ST-elevation myocardial infarction (STEMI) patients is related to healthcare costs and resources. This study aims to determine the underlying risk factors for longer LOS in STEMI patients at Chamran Hospital, Isfahan, Iran.</p><p><strong>Materials and methods: </strong>In this study, which is part of the STEMI cohort study (SEMI-CI) in Isfahan, Iran, patients with STEMI admitted to Chamran Hospital from March 2015 to March 2016 were identified. The association between risk factors and LOS was evaluated by trained nurses using medical archives. Logistic regression analysis identified independent predictors of LOS. Data were analyzed using SPSS Statistics 22 (IBM, Inc., Armonk, NY, USA), and the significance level was set at an alpha value of <0.05.</p><p><strong>Results: </strong>Of 867 STEMI patients, 760 had complete information. The median LOS for surviving patients was three days. Patients with longer hospitalization times were predominantly older, more likely to be female, had lower left ventricular ejection fraction (LVEF) and hemoglobin, a history of cardiovascular diseases (CVD), hypertension, and heart failure, and had previously used beta-blockers, statins, aspirin, angiotensin-converting enzyme inhibitors, and angiotensin receptor blockers. The results of logistic regression models showed that higher low-density lipoprotein cholesterol (LDL-C) (<i>P</i> = 0.032), lower hemoglobin (<i>P</i> = 0.004), and lower LVEF (<i>P</i> = 0.019) were three risk factors for increased length of hospitalization.</p><p><strong>Conclusion: </strong>Identifying and controlling the factors affecting the LOS of STEMI patients (such as LDL-C, hemoglobin levels, and LVEF) can help reduce LOS, decrease the overuse of medical resources, and improve patient recovery.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"27"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rogers Syndrome, an Uncommon Cause of Megaloblastic Anemia: A Case Series.","authors":"Reshma Roshan, Masarat Mehboob, Roman Latif, Javid R Bhat, Santosh G Rathod","doi":"10.4103/abr.abr_16_24","DOIUrl":"10.4103/abr.abr_16_24","url":null,"abstract":"<p><p>The present case series describes two male children of 5 months and 11 months old, having diabetes mellitus and megaloblastic anemia, but with no syndromic symptoms or organ involvement. The patient's clinical history prompted a targeted sequencing of the genes associated with monogenic diabetes. In first case, mutation in SLC19A2 gene (variant - c.391dupT [P.Tyr131leufs*8]), where a homozygous insertion of one base pair (bp) in exon 2 of SLC19A2 gene[c.391dupT] was found, resulted in shift of reading frame and termination of the protein chain downstream to codon 131 [p.Tyr131 LeufsTer8]. In the second case, of mutation in SLC19A2 gene (variant- c. 1002_1004del [p.Gly335del]), a homozygous deletion in exon 3 found. Both the patients were diagnosed with thiamine responsive megaloblastic anemia (TRMA). When patients present with diabetes mellitus, deafness, and megaloblastic anemia, the possibility of TRMA syndrome should be considered. This condition often responds to high-dose thiamine.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"24"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluoxetine Use for Very Young Children with Major Depression Disorder: A Pilot Randomized Clinical Trial.","authors":"Fereshteh Shakibaei, Mahdieh Soltanian, Alireza Abdellahi","doi":"10.4103/abr.abr_78_24","DOIUrl":"10.4103/abr.abr_78_24","url":null,"abstract":"<p><strong>Background: </strong>Major depressive disorder (MDD) as one of the disabling mental disorders has turned into one of the significant challenges for health systems worldwide. Although fluoxetine has been successfully applied to over 6-year-old individuals with MDD, its utility for younger patients has not been investigated yet. The current pilot study aims to assess fluoxetine use in 4-6-year-old children diagnosed with MDD.</p><p><strong>Materials and methods: </strong>The current double-blinded randomized clinical trial has been conducted on 40 four-to-six-year-old children with MDD who were assigned to one of the treatment groups with 0.7 mg/kg daily fluoxetine (n = 20) or placebo (n = 20) for 60 days. Preschool Feelings Checklist-Scale (PFC-S) and the antidepressant side effect checklist (ASEC) were assessed at study initiation, within 30 and 60 days after the intervention.</p><p><strong>Results: </strong>The PFC-S score significantly decreased in the fluoxetine-treated group (<i>P</i> value < 0.001), while those who received placebo revealed no change (<i>P</i> value = 0.317). The comparison of fluoxetine versus placebo treatment revealed a remarkable difference between the scores of PFC-S among the groups (<i>P</i> value < 0.001). Besides, the PFC-S scores were statistically lower among those treated with fluoxetine compared with placebo-treated ones in both 30 days (<i>P</i> value < 0.001) and 60 days (<i>P</i> value < 0.001) assessments. None of the patients presented any fluoxetine-related side effect.</p><p><strong>Conclusion: </strong>Based on the findings of this study, fluoxetine was a safe and efficient agent to manage depression in very young children; however, data in this regard are limited and inconclusive. Further studies are strongly recommended.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"22"},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of <i>Leishmania</i> Species on Microscopically Negative Smears in Gonbad-e-Kavus, Northeastern Iran.","authors":"Salehe Ostovan, Fatemeh Livani, Faramarz Koohsar, Roghiyeh Faridnia, Sima Besharat, Ganesh Yadagiri, Fatemeh Mesgariyan, Mohammad-Ali Mohaghegh, Hamed Kalani","doi":"10.4103/abr.abr_539_24","DOIUrl":"10.4103/abr.abr_539_24","url":null,"abstract":"<p><strong>Background: </strong>The diagnosis of cutaneous <i>leishmaniasis</i> (CL) is typically based on microscopic examination of Giemsa-stained smear. However, this method can lead to false negative and false positive results. Therefore, the study aimed to identify <i>L. major</i> and <i>L</i>. <i>tropica</i> species by investigating microscopically negative smears suspected of CL using the multiplex nested polymerase chain reaction (PCR) method.</p><p><strong>Materials and methods: </strong>This study was conducted on 227 microscopically negative specimens from individuals suspected of having CL referred to the CL Treatment and Diagnosis Center in Gonbad-e Kavus Health Center, Golestan Province, northeastern Iran. Multiplex nested PCR technique was utilized to detect <i>L. tropica</i> and <i>L. major</i> under investigation based on <i>ITS1</i> sequence.</p><p><strong>Results: </strong>2.64% (6/227) of specimens were detected as positive for <i>Leishmania</i> species, all of which were identified as <i>L</i>. <i>major</i>.</p><p><strong>Conclusion: </strong>The multiplex nested PCR technique demonstrated that microscopic examination of the smears is linked to false negative in diagnosing CL. Consequently, it is recommended to utilize the multiplex nested PCR method as either a primary diagnostic or a confirmatory approach for cases that are microscopically negative.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"17"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147500622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weekly Heart Rate Variability and Training Zone Analysis in U-15 Soccer Players: The Predictive Value of Combined Warm-up Protocols for Optimizing Cardiovascular Monitoring.","authors":"Hamzeh Zarei, Khosro Jalali Dehkordi, Farzaneh Taghian, Filipe Manuel Clemente","doi":"10.4103/abr.abr_388_25","DOIUrl":"10.4103/abr.abr_388_25","url":null,"abstract":"<p><strong>Background: </strong>Warm-up protocols play a crucial role in optimizing athletic performance and cardiovascular readiness in youth soccer players. This study aimed to compare the effects of six warm-up strategies on heart rate (HR) parameters and exertion levels in under-15 (U-15) soccer athletes.</p><p><strong>Materials and methods: </strong>Seventy-two male participants were randomly (concealed participant allocation) assigned to six groups: Dynamic Warm-up (DWU), Analytical Warm-up (AWU), Analytical + Dynamic Warm-up (ADWU), Small-Sided Games Warm-up (SSGWU), Integrated Warm-up (IWU), and Small-Sided Games + Integrated Warm-up (SIWU). This randomized parallel study lasted 8 weeks, with pre- and post-tests measuring resting HR, maximal HR (MHR), average HR (AHR), RR intervals, HR variability (HRV), session rating of perceived exertion (sRPE), and HR zone distributions.</p><p><strong>Results: </strong>Significant improvements (<i>P</i> < 0.001) were observed in resting, maximum, and average heart rates, RR intervals, HRV, and session RPE in most groups, especially DWU, SSGWU, and SIWU, indicating better cardiovascular function and reduced exertion. HR zone analysis showed significant shifts in all zones for IWU and SIWU (<i>P</i> < 0.001). Mixed repeated measures analysis of variance revealed significant between-group differences in post-test values for maximum HR, RR intervals, HRV, and sRPE change scores (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>These findings highlight the differential impact of warm-up protocols on cardiovascular outcomes and perceived exertion. Integrated and small-sided games warm-ups showed superior benefits, suggesting their incorporation into youth soccer training could optimize physiological preparedness and performance.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"20"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histogram Analysis and Heterogeneous Radial Changes Using ADC Map Images in Metastatic Brain Tumors for Dose Painting Intent.","authors":"Maryam Maleki, Tahereh Hadisinia, Iraj Abedi, Simin Hemati, Hamide Nematollahi, Alireza Amuheidari, Mohammad Baqer Tavakoli","doi":"10.4103/abr.abr_180_24","DOIUrl":"10.4103/abr.abr_180_24","url":null,"abstract":"<p><strong>Background: </strong>Our goal in this study was to examine the heterogeneous radial changes within tumors using histogram analysis and determine whether this method could assist in the implementation of targeted radiation painting.</p><p><strong>Materials and methods: </strong>The diffusion-weighted imaging image data for 25 patients with primary lung cancer was examined. For patients with multiple metastases, the largest tumor diameter was considered, and three different radial categories were selected to assess heterogeneity changes. Finally, the histogram parameters of apparent diffusion coefficient (ADC) were compared among the 25 patients, and the <i>P</i> values were obtained using SPSS software to judge among results.</p><p><strong>Results: </strong>The obtained outcome of our study demonstrated that in the central region of the tumor, up to a (0-0.3)r (tumor radius of the tumor), the tumor heterogeneity remains constant, and there is no significant difference in the ADC histogram percentiles of 25, 50, and 75. In the peripheral region of the tumor, at a distance away from the center, the heterogeneity level varies.</p><p><strong>Conclusions: </strong>These findings indicate that this method has the potential to separate the boundary between the center of the tumor and its periphery in terms of the level of heterogeneity, which is one of the requirements of dose painting.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"11"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose Prediction Deep Learning-Based Model for VMAT of Prostate Cancer Applying Magnetic Resonance Image (MRI) in Versa HD Linear Accelerator.","authors":"Hossein Taheri, Mohammadbagher Tavakoli, Khadijeh Mousavi, Hamed Taheri, Sheyda Lafz Lenjani, Maryam Farghadani","doi":"10.4103/abr.abr_180_25","DOIUrl":"10.4103/abr.abr_180_25","url":null,"abstract":"<p><strong>Background: </strong>Prostate cancer patients are commonly undergoing Radiotherapy (RT) and treatment planning system have a prominent role for dose calculation, while this would seem that dose distribution uncertainties of treatment planning system (TPS) may effect on RT results. Therefore, this study aimed to design a Dose prediction deep learning-based model for prostate cancer volumetric arc therapy (VMAT) applying MRI in Versa HD linear accelerator (linac).</p><p><strong>Materials and methods: </strong>In this work, MRI of 45 patients who underwent VMAT was acquired, and cycle-consistent GAN (CycleGAN) (that allow image-to-image translation) and U-net deep learning (DL) framework for prostate were employed. The synthetic CT (sCT) images were generated from MR images. The predicted dose among CycleGAN, U-net and Monaco TPS (that calculate dose distribution based on CT simulation images) was compared to each other.</p><p><strong>Results: </strong>The sCT that was generated employing CycleGAN illustrated more obvious boundaries than the sCT of U-net (sCTU-net). The gamma passing rate of cycleGAN and U-net was exceeded 97% and 90%, respectively, in all areas.</p><p><strong>Conclusion: </strong>The results of this study illustrates that deep learning models including CycleGAN and U-net are good alternative for dose prediction of VMAT in Versa HD linac, while it seems that CycleGAN may be more accurate compared to U-net.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"14"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moringa Oleifera Enhances the Cell Proliferation and Neural Differentiation of B65 and PC12 Cells.","authors":"Samira Shariati Najafabadi, Hajar Akbari Dastjerdi, Azadeh Safaee, Mohammad Kazemi, Hamid Bahramian, Hossein Salehi","doi":"10.4103/abr.abr_523_24","DOIUrl":"10.4103/abr.abr_523_24","url":null,"abstract":"<p><strong>Background: </strong>Neurodegenerative diseases, marked by progressive symptoms, currently lack definitive, side-effect-free treatments. Research continues for effective therapies. Moringa oleifera extract (MOE), rich in phytochemicals like vitamins, carotenoids, and flavonoids, has demonstrated neuroprotective effects through antioxidant activity and inflammation reduction. The aim of this study was to investigate the effect of Moringa extract on the proliferation and cell differentiation.</p><p><strong>Materials and methods: </strong>This study investigated the effects of MOE on the proliferation and cell differentiation of B65 and PC12 cells <i>in vitro.</i> MOE was extracted from plant leaves using the Soxhlet method, and its antioxidant properties were confirmed through the Ferric Reducing Antioxidant Power assay. Cell proliferation was assessed using an MTT assay, while cell differentiation was evaluated through Real-time PCR and immunocytochemistry (ICC).</p><p><strong>Results: </strong>MOE significantly increased cell proliferation in both cell lines in a dose-dependent manner. Real-time PCR results indicated that MOE upregulated the expression of the gap-43 gene, which encodes a protein, involved in regulating actin dynamics in axons and promoting axonal regeneration, as well as the nf200 gene, which encodes an intermediate filament protein in neurons. Furthermore, ICC results demonstrated that higher doses of MOE were associated with increased levels of the nf200 protein in both cell lines.</p><p><strong>Conclusion: </strong>The findings demonstrate that MOE improves cell proliferation and facilitates neuronal differentiation. These effects, attributed to its high antioxidant content, suggest that MOE may serve as a promising therapeutic candidate for neurodegenerative disorders.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"13"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Potentially Inappropriate Medication Prescriptions in Older Adults.","authors":"Mehdi Rezaee, Leila Ghamkhar, Mojtaba Sedaghat","doi":"10.4103/abr.abr_551_24","DOIUrl":"10.4103/abr.abr_551_24","url":null,"abstract":"<p><strong>Background: </strong>By 2050, the global older adult population is expected to exceed 1.5 billion. An issue of concern among this demographic is the prevalent use of potentially inappropriate medications (PIMs), leading to adverse drug reactions and hospitalizations. In Iran, over 3.5 million individuals aged 65 years and above are insured by the Iran Health Insurance Organization (IHIO), constituting around 8% of the total population. This study evaluated PIM prescribing practices for Iranian older adults covered by IHIO, using the updated Beers Criteria (2023).</p><p><strong>Materials and methods: </strong>In this retrospective cross-sectional study, electronic prescription data for older adult IHIO beneficiaries aged 65 years and above were analyzed using the Cross Industry Standard Process for Data Mining over a 1-year period. The Beers criteria 2023 was applied to identify PIMs.</p><p><strong>Results: </strong>The study included 12,821,179 prescriptions from 2,589,595 patients, revealing polypharmacy in 38.72% of prescriptions. Approximately 25.76% of all prescriptions contained at least one PIM, with ketorolac being the most commonly prescribed PIM (25.17% of all PIM prescriptions). Cardiologists exhibited the highest rate of PIM prescribing behavior at 34.95%, followed closely by general practitioners at 34.46%.</p><p><strong>Conclusions: </strong>The study emphasizes the significance of addressing PIM prescriptions in outpatient settings. Healthcare professionals can improve the safety and well-being of older patients undergoing medical treatment by implementing strategies to identify and minimize PIM.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"19"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}