Advanced biomedical research最新文献

{"title":"Lung Abscess in a COVID-19 Patient: A Case Report.","authors":"Hazhir Moradi, Somayeh Haghighipour","doi":"10.4103/abr.abr_88_23","DOIUrl":"10.4103/abr.abr_88_23","url":null,"abstract":"<p><p>Coronavirus disease 2019 (COVID-19) has many complications and also atypical presentations. Here, we reported a 60-year-old female who was admitted to our center complaining of headache and dyspnea. The initial high-resolution computed tomography (HRCT) scan revealed an abscess in the middle lobe of the right lung and also patchy pleural base ground-glass opacity consistent with COVID-19 infection. A treatment regimen, including dexamethasone, enoxaparin, piperacillin/tazobactam, and famotidine intravenous remdesivir was started. The HRCT scan was repeated on the 6^th day of admission and showed the same ground-glass opacity as before but the lung abscess had become smaller in size. Altogether, we should be aware of lung abscess as one of the COVID-19 manifestations and complications.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"9"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Epidemiological Characteristics of Ventilator-associated Pneumonia in Pediatric Intensive Care Units.","authors":"Shahareh Babai, Mojtaba Parastegari, Nazanin Zibanejad","doi":"10.4103/abr.abr_24_25","DOIUrl":"10.4103/abr.abr_24_25","url":null,"abstract":"<p><strong>Background: </strong>Ventilator-associated pneumonia (VAP) is the leading cause of antibiotic use in pediatric intensive care units (PICUs). Given its high prevalence and significant treatment costs, there is a pressing need for comprehensive epidemiological and clinical investigations into this condition. The aim of this study is to determine the frequency of VAP and its association with factors such as age, sex, and the initial diagnosis in patients undergoing mechanical ventilation in the intensive care units (ICU).</p><p><strong>Materials and methods: </strong>In this study, VAP cases were initially diagnosed based on the clinical and radiological criteria established by the department of pediatric (CDC). Subsequently, the frequency of VAP-associated pathogens and their antimicrobial resistance profiles were assessed in samples obtained from bronchoalveolar lavage (BAL) fluids.</p><p><strong>Results: </strong>Among 119 children who received mechanical ventilation in the ICU of Imam Hossein Hospital, 49 (41.2%) were diagnosed with VAP. Gram-negative organisms were the most common pathogens, with Acinetobacter (30.6%), Klebsiella (18.4%), and Pseudomonas (10.2%) being the most prevalent. The mortality rate among VAP patients (38.8%) was comparable to that of non-VAP patients (38.7%).</p><p><strong>Conclusion: </strong>Patients with VAP had significantly longer durations of mechanical ventilation, ICU stay, and overall hospitalization.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"15"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Findings, Antibody Panel and Pathology of Patients with Inflammatory Myopathies in Isfahan Province, Iran.","authors":"Behnaz Ansari, Keivan Basiri, Faeze Soroush","doi":"10.4103/abr.abr_304_23","DOIUrl":"10.4103/abr.abr_304_23","url":null,"abstract":"<p><strong>Background: </strong>In the current study, we aimed to evaluate the characteristics of individuals with autoimmune inflammatory myopathies (AIMs) in Isfahan.</p><p><strong>Material and methods: </strong>In this retrospective, observational study, the clinical, histopathological, immunohistochemical staining, electromyography, and autoantibody profile of the patients with AIM in subgroups of dermatomyositis (DM), necrotizing autoimmune myopathy (NAM), polymyositis (PM), and antisynthetase syndrome (AS) were assessed.</p><p><strong>Results: </strong>Seventy-seven patients with AIMs including 38 (49.3%), 21 (27.3%), 14 (18.2%), and 4 (5.2%) with NAM, DM, PM, and antisynthetase diagnoses were enrolled, respectively. Proximal weakness (94.80%), myalgia (32.46%), skin rash (25.97%), and dysphagia (25.97%) were the most common presentations, among which skin rash was statistically more frequent in DM (<i>P</i> value = 0.001). Fiber necrosis (100%), CD68 (92.1%), and C5-b9 (60.5%) positivity, irritable myopathy (78.9%), and anti-Ro-52 (7.1%) positivity were the most common findings in NAM. Perivascular infiltration (71.4%), fiber degeneration (66.7%) and necrosis (52.4%), CD68 (100%), CD20 (71.4%), and CD8 (71.4%), both irritable (57.1%) and non-irritable (38.1%) myopathy, anti-Mi-2 (19%), anti-NXP-2 (19%), and anti-MDA-5 (14.3%) autoantibodies were the detected characteristics of DM patients. PM individuals were characterized with endomysial infiltration (92.9%), fiber necrosis (71.4%) and perivascular infiltration (64.3%), CD68 (100%), CD20 (100%) and CD8 (92.9%) positivity, both irritable (57.1%) and non-irritable (42.9%) myopathy and anti-Mi-2 (14.3%), and anti-ANA (14.3%) antibodies. Fiber necrosis (100%) and degeneration (75%), CD68 (75%), CD8 (75%), and C5-b9 (75%), irritable myopathy (50%), and anti-Jo-1 antibody (100%) positivity were the diagnostic tools for AS.</p><p><strong>Conclusion: </strong>According to the outcomes of the current study, the clinical, histopathological, and autoantibody profiles of the patients with AIM are variably different with remarkable overlaps. Therefore, thorough assessments are required to make the final diagnosis.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"12"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential Anti Stress-like Properties of Escitalopram on Improvement of Spatial Memory in Rats Subjected to Predictable and Unpredictable Chronic Mild Stress.","authors":"Vajihe Saedi-Marghmaleki, Maryam Radahmadi, Hojjatallah Alaei, Hossein Khanahmad","doi":"10.4103/abr.abr_654_24","DOIUrl":"10.4103/abr.abr_654_24","url":null,"abstract":"<p><strong>Background: </strong>Chronic stress and chronic escitalopram usage affect memory. This research examined the potential anti-stress-like properties of escitalopram in improving spatial memory in rats subjected to predictable and unpredictable chronic mild stress (PCMS and UCMS).</p><p><strong>Materials and methods: </strong>A total of forty-nine male rats were assigned to various groups: control (Co), sham (Sh), predictable stress (P.St; 2 h daily/21 days), unpredictable stress (UP.St; 2 h daily/21 days), escitalopram (Esc; 10 mg/kg/day for 21 days), and stress groups with escitalopram (P.St-Esc and UP.St-Esc). The Barnes maze test evaluated spatial learning and memory across all groups. Subsequently, the hippocampal weight was measured.</p><p><strong>Results: </strong>Both the P.St and UP.St groups showed significant (<i>P</i> < 0.01 and <i>P</i> < 0.001, respectively) improvements in escape latency and traveled distance compared to the control group. It is important to note that only the P.St-Esc group showed decreases (<i>P</i> < 0.05 in both) in these variables when compared to the P.St group, whereas no significant differences were found between the UP.St and UP.St-Esc groups. In the P.St and UP.St groups, the left hippocampal weight was significantly (<i>P</i> < 0.05 and <i>P</i> < 0.01; respectively) lower than that of the control group.</p><p><strong>Conclusion: </strong>Both PCMS and UCMS adversely affected spatial learning and memory. Additionally, escitalopram improved spatial learning and memory only in rats subjected to PCMS conditions. Therefore, it seems that escitalopram improves learning and memory by different pathways in the PCMS and UCMS conditions. Consequently, it seems that different dosages of escitalopram are required to treat memory deficits due to various types of stress. Left hippocampal atrophy also confirmed the potential role of UCMS conditions in memory impairments.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"18"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association between Triglycerides to High-density Lipoprotein Cholesterol (TG/HDL-C) Ratio and 10-year Prospective Risk of Type 2 Diabetes Mellitus: Yazd Healthy Heart Project.","authors":"Mohammad Poorebrahimi, Seyed Reza Mirjalili, Sepideh Soltani, Pedro Marques-Vidal, Seyedeh Mahdieh Namayandeh, Mohammadtaghi Sarebanhassanabadi","doi":"10.4103/abr.abr_221_25","DOIUrl":"10.4103/abr.abr_221_25","url":null,"abstract":"<p><strong>Background: </strong>Although the link between dyslipidemia and type 2 diabetes mellitus (T2DM) is well-established, the predictive value of the triglyceride to high-density lipoprotein cholesterol (TG/HDL-C) ratio remains unclear. Most existing evidence comes from East Asian populations, highlighting the need for data from more diverse groups. This study investigates the association between TG/HDL-C ratio and T2DM risk in a broader population and aims to identify a practical cut-off point for predicting T2DM incidence.</p><p><strong>Materials and methods: </strong>Cohort study using data from the Yazd Healthy Heart Project (YHHP). Two thousand people aged 20 to 74 were followed for an average of 9.9 years. After excluding individuals with diabetes and missing data, 1,107 participants remained. Multivariate Cox proportional hazard models were applied to examine the relationship between TG/HDL-C ratio and T2DM incidence.</p><p><strong>Results: </strong>In the crude analysis, individuals in the highest quartile of TG/HDL-C ratio had a higher risk of T2DM compared to the lowest quartile, with hazard ratios (HR) of 2.52 (95% CI: 1.33-4.70) in males and 4.05 (95% CI: 2.17-7.57) in females. Adjusted HRs for the highest quartile versus lowest were 1.28 (95% CI: 0.45-3.57) in males and 6.74 (95% CI: 1.43-31.8) in females, with a significant trend in females (<i>P</i> = 0.018) but not in males (<i>P</i> = 0.080). A TG/HDL-C ratio ≥2.46 showed good sensitivity and specificity for predicting T2DM.</p><p><strong>Conclusions: </strong>Increased TG/HDL-C ratio was associated with higher risk of new-onset T2DM, especially in females, supporting its potential role as a biomarker for T2DM risk.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"16"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustained Lung Inflation in Preterm Infants with Signs of Respiratory Distress: A Randomized Controlled Trial.","authors":"Majid Mohammadizadeh, Mehrnaz Moeini, Behzad Barekatain","doi":"10.4103/abr.abr_526_23","DOIUrl":"10.4103/abr.abr_526_23","url":null,"abstract":"<p><strong>Background: </strong>The role of sustained lung inflation (SI) in neonatal resuscitation at birth as a substitute for, or an adjuvant to positive pressure ventilation (PPV) has been previously examined. This study was conducted to examine the effect of applying SI in preterm infants, who did not need PPV at birth but showed signs of respiratory distress within the first minutes of life, on their mortality and some common morbidities.</p><p><strong>Materials and methods: </strong>In this double-blind randomized clinical trial, seventy-four preterm infants with gestational age 28-33 6/7 weeks and birth weight more than 1000 g in whom signs of respiratory distress appeared within five to ten minutes after birth were randomly assigned to receive nCPAP alone (nCPAP-only group) or a 10-second sustained inflation with 20 cmH₂O pressure followed by nCPAP (SI group).</p><p><strong>Results: </strong>The need for surfactant administration was significantly less (<i>P</i> = 0.003) and the length of hospital stay was significantly shorter in the SI group (<i>P</i> = 0.005), but the duration of nCPAP was significantly longer in this group (<i>P</i> = 0.003). There was no statistically significant difference between the two groups in terms of the need for and duration of mechanical ventilation and also, other secondary outcomes.</p><p><strong>Conclusions: </strong>Applying SI within the first minutes after birth in preterm infants with early signs of respiratory distress can reduce the need for exogenous surfactant and the length of hospital stay. Further studies on the role of this modality in the management of RDS in neonates, especially its effect on long-term cardiopulmonary and neurodevelopment outcomes, are recommended.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"10"},"PeriodicalIF":1.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study of the Effect of Novel Dual Trigger and Delayed HCG With Dual Trigger in Poor Responder Poseidon Group 4 Patients in Antagonist IVF Cycles.","authors":"Elham Naghshineh, Farnoosh Farahbod, Hatav G Tehrani, Ferdous Mehrabian","doi":"10.4103/abr.abr_286_25","DOIUrl":"10.4103/abr.abr_286_25","url":null,"abstract":"<p><strong>Background: </strong>Decreased Ovarian Reserve (DOR) is a significant challenge in infertility treatment. This study, which compared the effects of Novel Dual Trigger and Delayed Human Chorionic Gonadotropin (HCG), holds promise for improving IVF outcomes.</p><p><strong>Materials and methods: </strong>This was a randomized, double-blinded clinical trial on 90 infertile women. An antagonist regimen was used, which included approximately 300 units of gonadotropins, consisting of 150 units of Human menopausal gonadotropin (hMG) and 150 units of Synal-F. After administering a dose of Strotide injection followed by Decapeptyl and Ovitrelle injections, 12 hours later, a single HCG 5000-unit injection was administered, and 36 hours later, the puncture procedure was performed.</p><p><strong>Results: </strong>Although the frequency of chemical pregnancy, Implantation rate, and ongoing pregnancy was higher in the Novel double trigger with delayed HCG group, and the frequency of zero oocytes and fetus was lower in this group, this difference was not statistically significant.</p><p><strong>Conclusion: </strong>This study showed that using Novel Dual Trigger and Delayed HCG, compared to Dual Trigger, did not significantly differ in the number of oocytes, embryos, or secondary pregnancy outcomes. These findings suggest that the Novel Dual Trigger and Delayed HCG methods could be equally effective in improving IVF outcomes for poor responder patients, providing a potential alternative for ovarian stimulation in these cases and instilling optimism for the future of IVF treatments.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"3"},"PeriodicalIF":1.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the 5-Year Incidence of Dyslipidemia in the Obese and Nonobese Adult Population: Results of the Yazd Health Study (YaHS).","authors":"Parisa Peigan, Masoud Mirzaei, Pedro Marques-Vidal, Mohammadtaghi Sarebanhassanabadi","doi":"10.4103/abr.abr_140_25","DOIUrl":"10.4103/abr.abr_140_25","url":null,"abstract":"<p><strong>Background: </strong>Dyslipidemia is a disease and conditions associated with obesity, which is a chronic health problem. The study aimed to determine the 5-year incidence of dyslipidemia in obese and nonobese participants in Central Iran.</p><p><strong>Materials and methods: </strong>Using multistage cluster sampling, 9964 participants aged 20-70 years in Yazd, Iran, were recruited between 2014 and 2015. Those with dyslipidemia were excluded, and nondyslipidemia individuals were included in the study. After a 5-year follow-up, the obese and nonobese participants were evaluated for incident dyslipidemia. We used logistic regression to estimate the 5-year incidence rates of dyslipidemia.</p><p><strong>Results: </strong>During the study's follow-up, dyslipidemia was diagnosed in 4.4% of participants (n = 355). The increase in the incidence of dyslipidemia in obese women was 86.1% (179 people) more than in obese men, with 32.7% (n = 48). The adjusted odds ratios (ORs) (95% confidence intervals [CIs]) for dyslipidemia in obese men and women were 1.32 (0.90-1.94) and 2.81 (1.78-4.43). The results of the logistic regression analysis showed that obesity was associated with a higher risk of dyslipidemia in the participants after adjusting for confounding factors. However, there was no difference in the findings between those under 60 and those over 60.</p><p><strong>Conclusions: </strong>The cohort study indicated that during a 5-year period, obesity was linked to a higher incidence of dyslipidemia in the adult population. Thus, by combining effective policies with obesity prevention initiatives, it is possible to reduce the incidence of dyslipidemia, cardiovascular illnesses, and the mortality associated with these conditions.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"6"},"PeriodicalIF":1.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and Opportunities in the Development of Dengue Vaccines: A Comprehensive Review.","authors":"Razieh Moghadasi, Yasaman Yadollahi Fars, Hamid Nasri, Mahnaz Momenzadeh","doi":"10.4103/abr.abr_643_24","DOIUrl":"10.4103/abr.abr_643_24","url":null,"abstract":"<p><p>The dengue virus is the cause of dengue fever, a disease that is prevalent in many regions around the world. Dengue fever is a significant public health concern, particularly in tropical and subtropical areas. The virus has four distinct serotypes and is transmitted to humans through the bites of infected Aedes mosquitoes, which can result in potentially fatal infections. There is currently no specific antiviral treatment for this disease; management mainly involves supportive care for those experiencing symptoms. Therefore, prevention is one of the most effective strategies for controlling this infection. The most effective measure for preventing the disease is vaccination. In recent years, substantial efforts have been made to develop a vaccine effective against all four serotypes of the virus. To date, only one vaccine has been approved, while several others are in phase II and III clinical trials to confirm their efficacy. The development of a dengue vaccine faces several challenges, such as the need for effectiveness against all serotypes, issues related to antibody-dependent enhancement, cross-reactive immune responses, and age-related efficacy limitations. This paper examines the key strategies for vaccine development and the latest advancements in creating an effective dengue vaccine.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"8"},"PeriodicalIF":1.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a Vaccine for the Monkeypox Virus Using Immunoinformatics and Structural Tools.","authors":"Razieh Dowran, Behzad Dehghani, Mohammad Javad Rasaee, Mehdi Norouzi, Seyed Mohammad Jazayeri","doi":"10.4103/abr.abr_27_25","DOIUrl":"10.4103/abr.abr_27_25","url":null,"abstract":"<p><strong>Background: </strong>Monkeypox virus (MPXV) causes mpox, and vaccination is the most effective method of prevention. This project utilized immunoinformatics tools to design a multiepitope subunit vaccine against Mpox.</p><p><strong>Materials and methods: </strong>Seven MPXV antigens (B18R, B21R, D2L, E8L, N3R, I1L, and N2R) were selected based on a literature review, and their sequences were retrieved with the signal peptides and transmembrane domains omitted. B cell, T cell, MHC-I, and MHC-II epitopes were evaluated, followed by tertiary structure and homology modeling. The physicochemical properties of the construct were assessed, molecular docking was conducted with murine Toll-like receptors, and codon optimization was performed.</p><p><strong>Results: </strong>Three sequences were excluded: B18R lacked appropriate epitopes, N2R was predicted to be an allergen by AllergenFP software, and I1L lacked antigenicity. The final vaccine construct incorporated segments from four genes, along with two adjuvants and two T-helper molecules linked by short sequences. The designed protein had a pI of 7.96, a molecular weight of approximately 16 kDa, an energy value of -723.06, a codon frequency distribution of 100, and a codon adaptation index of 0.88. The construct was subsequently cloned and inserted into a pET-28a(+) vector to generate a recombinant plasmid.</p><p><strong>Conclusions: </strong>Using immunoinformatics techniques, a novel multiepitope vaccine construct for MPXV was successfully designed.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"15 ","pages":"5"},"PeriodicalIF":1.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}