Advanced biomedical research最新文献

{"title":"The Interaction of Zinc as an Essential Trace Element with <i>Leishmania</i> Parasites: A Systematic Review.","authors":"Maryam Aghaei, Shahrzad Aghaei, Mahbobeh Kouhiyan, Zabihollah Shahmoradi, Seyed H Hejazi","doi":"10.4103/abr.abr_187_23","DOIUrl":"10.4103/abr.abr_187_23","url":null,"abstract":"<p><strong>Background: </strong>The trace element of zinc (Zn) has shown great effectiveness in control of leishmaniasis infection. Hence, the present study conducted a systematic review of in vitro and in vivo studies evaluating the zinc effect in the treatment or prevention of leishmaniasis.</p><p><strong>Materials and methods: </strong>A systematic literature search was performed of all articles published in PubMed, SciELO, ScienceDirect, Scopus, Google Scholar, and Web of Science databases (1997-2023). The search terms were \"zinc\" OR \"cutaneous leishmaniasis (CL)\" OR \"visceral leishmaniasis (VL)\".</p><p><strong>Results: </strong>Initial search yielded 89 citations, and 59 subjects were included. Data showed the zinc serum level in CL patients was lower than controls. Also, <i>in vitro</i> studies of zinc were more effective against <i>L. tropica</i> and <i>L. major</i> promastigotes compared to the amastigotes. Moreover, in vivo studies did not show destructive effects of zinc on the mammalian cell viability like macrophages. Furthermore, zinc depletion by specific chelators affected <i>L. donovani</i> survival and growth through promoting apoptosis and reactive oxygen species-dependent mechanisms.</p><p><strong>Conclusion: </strong>The serum level determination of zinc could be useful for estimating the leishmaniasis pathophysiology. Environmentally or genetically determined increases in zinc levels might augment resístanse to CL. In contrast, zinc depletion using a zinc-specific chelator could be effective treatment of VL in endemic areas.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"73"},"PeriodicalIF":0.7,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Angiotensin II Type 1 Receptor Antagonist on Age-Related Differences in Renal Vascular Responses to Angiotensin II in Male and Female Rats.","authors":"Fatemeh Eshraghi-Jazi, Mehdi Nematbakhsh","doi":"10.4103/abr.abr_387_23","DOIUrl":"10.4103/abr.abr_387_23","url":null,"abstract":"<p><strong>Background: </strong>Advancing age could influence renin angiotensin system components, especially angiotensin type 1 receptor (AT1R). This study examined the effect of AT1R antagonist, losartan, on age-related differences in renal vascular responses to angiotensin II in male and female rats.</p><p><strong>Materials and methods: </strong>Forty-eight anesthetized male and female rats (8-12 and 24-28 weeks age ranges) were subjected to catheterize. Then, the responses of mean arterial pressure (MAP), renal perfusion pressure (RPP), renal blood flow (RBF), and renal vascular resistance (RVR) to angiotensin II with or without losartan were determined and evaluated.</p><p><strong>Results: </strong>There were not significant differences in the basal values of MAP, RPP, RBF, and RVR in males. However, it was observed significant difference in RVR in females (<i>P</i> < 0.05). The blockade of AT1R attenuated basal MAP and RPP in all the groups (<i>P</i> < 0.05). The infusion of losartan altered basal RVR and RBF values in female groups (<i>P</i> < 0.05). Moreover, losartan eliminated vasoconstrictor responses to angiotensin II in female groups (<i>P</i> < 0.05). Also, losartan induced significant vascular responses to angiotensin II in male groups (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>Losartan could maintain RBF changes in response to angiotensin II in both 8-12- and 24-28-week females. Losartan enhanced the RBF response to angiotensin II in 8-12-week males, but not in 24-28-week males. It seems that females (not males) in various age ranges are resistance against RBF changes by acutely increased angiotensin II.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"71"},"PeriodicalIF":0.7,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Family History of Stuttering among Kurdish Children Who Stutter Near the Age of Onset.","authors":"Hiwa Mohammadi, Parya Bakhtiary","doi":"10.4103/abr.abr_233_23","DOIUrl":"10.4103/abr.abr_233_23","url":null,"abstract":"<p><strong>Background: </strong>The family history of stuttering is one of the most important factors for the prediction of recovery from stuttering. There is a high controversy about the rate of positive family history of stuttering among people who stutter (PWS). In the present study, the family history of stuttering in the first-, second-, and third-degree relatives was investigated among a group of Kurdish children who stuttered (CWS) close to the onset of the disorder.</p><p><strong>Materials and methods: </strong>One hundred and seventy-two CWS, including 46 females (24.60%) aged 2-5 years (mean age: 3.71 years, SD = 0.75) were consecutively recruited. They were first examined close to the onset of the stuttering. Data about current and former stuttering among first-, second-, and third-degree relatives were collected using interviews with both parents. Data was described and analyzed by independent sample <i>t</i> test or Chi-square.</p><p><strong>Results: </strong>Among 172 CWS (mean age: 3.71 years, SD = 0.75), 119 (69.20%) reported a family history of stuttering. According to the result, the most family history of stuttering was reported among third-degree relatives (32.60%). Sixty-five participants (37.80%) reported just one relative with stuttering. CWS with a positive family history of stuttering had no significant difference with CWS without a positive family history of stuttering in sex (<i>P</i> = 0.48) and age of stuttering onset (<i>P</i> = 0.96).</p><p><strong>Conclusion: </strong>The rate of positive family history of stuttering among Kurdish CWS is similar to previously reported data in the same age group. There was no significant association of family history of stuttering with sex and age on the onset of the disorder.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"68"},"PeriodicalIF":0.7,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Index for Assessment of Severity and Extent of Gingival Pigmentation; A Diagnostic Cross-Sectional Study According to Inter and Intra-Observer Variability.","authors":"Azadeh Esmaeil Nejad, Mansoor Meimandi, Hasti Yaghmaei, Masoud Hatami, Saeed Madihi","doi":"10.4103/abr.abr_324_23","DOIUrl":"10.4103/abr.abr_324_23","url":null,"abstract":"<p><strong>Background: </strong>Classification of gingival pigmentation (GP) is often performed using gingival pigmentation index (GPI), melanin pigmentation index (MPI), oral pigmentation index (DOPI), melanin index (MI), or physiologic/pathologic GPI. However, the internal and external reproducibility of these indexes have not been evaluated. This study aimed to introduce a new simple and applicable GPI and assess its internal and external reproducibility.</p><p><strong>Materials and methods: </strong>This diagnostic cross-sectional study was conducted on 40 patients with maxillary and mandibular GP. Patients were in the age range of 12-60 years, and degree of GP was determined by four periodontists using DOPI, MI, MPI, GPI and the new index namely the severity and extension of gingival melanotic pigmentation index (SEMPI) under similar environmental and lighting conditions. The inclusion criteria were in the age range of 12-60 years and presence of melanin pigmentation (physiologic or pathologic) in the gingival margin, papilla, or attached gingiva. The exclusion criteria were systemic diseases, vascular lesions, hemochromatosis, amalgam tattoo, hyperbilirubinemia, or use of medications causing nonmelanotic pigmentation (hemoglobin, iron, and amalgam).</p><p><strong>Results: </strong>The Fleiss kappa between all examiners for all indexes at 0, 7, and 14 days was higher than 0.8, indicating high agreement among the examiners. The Spearman's correlation coefficient for all indexes was positive and high at 0, 7, and 14 days (<i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>Considering the high agreement among the examiners for all indexes and at all-time points as well as the positive, strong correlations among them, it seems that the new index is useful for classification of GP.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"63"},"PeriodicalIF":0.7,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimethyl Fumarate Preconditioning can Reinforce the Therapeutic Potential of Bone Marrow Mesenchymal Stem Cells through Trophic Factor Profile Enhancement.","authors":"Sareh Pandamooz, Anahid Safari, Nasrin Ghorbani, Iman Jamhiri, Shahrokh Zare, Ivaldo Jesus Almeida Belém-Filho, Parisa Dolati, Mohammad Saied Salehi","doi":"10.4103/abr.abr_298_23","DOIUrl":"10.4103/abr.abr_298_23","url":null,"abstract":"<p><strong>Background: </strong>Numerous studies have confirmed the therapeutic efficacy of bone marrow-derived mesenchymal stem cells (BM-MSCs) in addressing neurologic disorders. To date, several preconditioning strategies have been designed to improve the therapeutic potential of these stem cells. This study was designed to evaluate the preconditioning effect of dimethyl fumarate (DMF) on the expression of main trophic factors in human BM-MSCs.</p><p><strong>Materials and methods: </strong>Initially, the identity of stem cells was confirmed through the evaluation of surface markers and their capacity for osteogenic and adipogenic differentiation using flow cytometry and differentiation assay, respectively. Subsequently, stem cells were subjected to different concentrations of DMF for 72 hours and their viability was defined by MTT assay. Following 72-hour preconditioning period with 10 µM DMF, gene expression was assessed by quantitative RT-PCR.</p><p><strong>Results: </strong>Our findings demonstrated that the isolated stem cells expressed cardinal MSC surface markers and exhibited osteogenic and adipogenic differentiation potential. MTT results confirmed that 10 µM DMF was an optimal dose for maintaining cell viability. Preconditioning of stem cells with DMF significantly upregulated the expression of <i>BDNF</i>, <i>NGF</i>, and <i>NT-3</i>. Despite a slight increase in transcript level of <i>GDNF</i> and <i>VEGF</i> after DMF preconditioning, this difference was not statistically significant.</p><p><strong>Conclusions: </strong>Our findings suggest that DMF preconditioning can enhance the expression of major neurotrophic factors in human BM-MSCs. Given the curative potential of both BM-MSCs and DMF in various neurological disease models and preconditioning outcomes, their combined use may synergistically enhance their neuroprotective properties.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"37"},"PeriodicalIF":0.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11368223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142121533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Thyroid Nodules and Predictive Factors for their Malignancy: Is there any Correlation between Level of TSH and Type of Malignancy?: A Cross Sectional Study.","authors":"Leila Moradi, Mohsen Hani Tabaei Zavareh, Ferdos Zaman, Anahita Shareghi Bruojeni, Seyed Amir Mohammad Taravati, Hossein Naderi Boldaji, Koushan Rostami, Seyedeh Ghazal Shahrokh","doi":"10.4103/abr.abr_158_23","DOIUrl":"https://doi.org/10.4103/abr.abr_158_23","url":null,"abstract":"<p><strong>Background: </strong>Thyroid nodule is a common finding of the endocrine system. Despite thyroid nodules in most cases being symptom-free and mostly benign, there are always malignancy risks. This study aimed to evaluate the frequency of types of thyroid nodules according to histopathology characteristics in Ahvaz hospitals.</p><p><strong>Materials and methods: </strong>This retrospective cross-sectional study was performed on patients with thyroid nodules with histopathological diagnosis referred to the educational hospitals of Ahvaz Jundishapur University of Medical Sciences and private endocrine clinics in Ahvaz from 2018 to 2021. Demographic characteristics, clinical history, histopathological results, ultrasonographic findings, and the presence of lymphadenopathy were collected from the patient's medical records.</p><p><strong>Results: </strong>A total of 380 patients with thyroid nodules with a mean age of 41.2 ± 12.28 years, 79.37% of whom were female were included in the study. A total of 267 (70/3%) cases were benign. Malignancy was found in 113 (29.7%) patients in which papillary thyroid cancer was the commonest histological diagnosis (<i>n</i> = 99; 87.61%). Age between 30 and 45 years (<i>P</i> = 0.001), bilateral involvement (<i>P</i> = 0.028), nodules size (<i>P</i> = 0.004), and lymph node involvement (<i>P</i> < 0.0001) were significantly associated with malignancy of thyroid nodules. However, there was no significant relationship between malignancy with gender (<i>P</i> = 0.450), BMI (<i>P</i> = 0.258), and history of head and neck radiotherapy (<i>P</i> = 0.200). There was no significant relationship between high thyroid stimulating hormone (TSH) and the type of malignancy (<i>P</i> = 0.059).</p><p><strong>Conclusion: </strong>Malignant thyroid nodules were associated with factors including age between 30 and 40 years, bilateral nodules, and lymphadenopathy. Therefore, it is important to consider these risk factors in the management and risk assessment of thyroid nodules.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"48"},"PeriodicalIF":0.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11478690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Central Venous Catheter Complications in the Poisoning Emergency Center: A 5-Year Cross-Sectional Study.","authors":"Amirmohammad Bavandipour, Masumeh Safaee, Mohammad Javad Tarrahi, Nastaran Eizadi-Mood","doi":"10.4103/abr.abr_218_22","DOIUrl":"https://doi.org/10.4103/abr.abr_218_22","url":null,"abstract":"<p><strong>Background: </strong>Central venous catheters (CVCs) are used widely in emergency centers, which may be associated with complications. There is a paucity of literature focusing on CVC complications, specifically in the poisoning emergency centers. In this study, we determined the frequency of CVC complications in patients with acute poisoning.</p><p><strong>Materials and methods: </strong>This was a cross-sectional study performed in the poisoning referral center. We reviewed the medical records of patients with acute poisoning who underwent CVC at the time of admission/during hospitalization in the poisoning intensive care unit or poisoning ward and were hospitalized between 2014 and 2019.</p><p><strong>Results: </strong>During the study period, 33, l37 patients with acute poisoning had been admitted and CVC was placed for 400 patients (1.20% of total patients). Most of the CVCs had been placed via femoral (51%) (204 cases). The frequency of CVC complications was 13.75%. The CVC in the internal jugular (IJ) vein was associated with a higher complication (20.7%) (<i>P</i> value 0.02). Infection (9.2%) and pneumothorax (9.2%) were more observed with the IJ approach, while arterial puncture was more observed with subclavian (3.7%) (<i>P</i> value < 0.0001).</p><p><strong>Conclusions: </strong>The frequency of CVC placement in acute poisoning patients was not noticeable. Although the femoral vein was the most commonly used approach in our institution, overall complications were more observed with the IJ vein approach.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"58"},"PeriodicalIF":0.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11478964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison the Effect of Rehabilitation at Home and Outpatient Physiotherapy after Total Knee Arthroplasty Surgery on Quality of Life and Knee Function: A Clinical Trial Study.","authors":"Babak Haghpanah, Fatemeh Tavakoli, Mohammadhadi Mollaabbasi, Mohammad J Azadchehr, Ali Afsharirad, Amirhossein Sadeghian, Amirmohammad Taravati, Seyedeh Ghazal Shahrokh, Abdollah Shakiba Dastgerdi","doi":"10.4103/abr.abr_177_23","DOIUrl":"https://doi.org/10.4103/abr.abr_177_23","url":null,"abstract":"<p><strong>Background: </strong>Primary total knee arthroplasty (TKA) is an effective procedure to better function and relief the pain in advanced osteoarthritis patients. This study aimed to evaluate the effectiveness and safety of outpatient clinic-based setting of physiotherapy versus home-based rehabilitation (HBR) for functional recovery immediately after primary TKA procedure.</p><p><strong>Materials and methods: </strong>This randomized clinical trial study was conducted on patients underwent TKA in 2021-2022. Participants were randomly allocated to either \"usual care\" or a \"home-based rehabilitation\" in a 1:1 ratio. The primary outcome measurement of this study was the Knee Society Score (KSS). The secondary outcome was patient quality of life using the 36-item Short Form Survey Instrument (SF-36). Data were entered into the SPSS software 25 and analyzed.</p><p><strong>Results: </strong>In this study, 80 patients who underwent TKA in equal proportion in two outpatient physiotherapy (36 women; average age 64.37 ± 5.12 years) and home rehabilitation (33 women; average age 62.4 ± 4.87 years) groups were examined. The findings our study showed that after the intervention (3 and 5 months after the operation), there was no significant difference between the two groups in terms of the quality of life (total score) (<i>P</i> > 0.05). KSS had no significant difference between the two groups (<i>P</i> > 0.05).</p><p><strong>Conclusion: </strong>This randomized controlled trial found no significant differences in the outcomes of rehabilitation using either a HBR or a traditional outpatient physiotherapy model.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"51"},"PeriodicalIF":0.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11478725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of Laparoscopic Approach to Cesarean Scar Pregnancy (CSP).","authors":"Safoura Rouholamin, Nasrin Ghasemi Toudeshkchuei, Maryam Hashemi, Zahra Allameh, Elham Naghshineh","doi":"10.4103/abr.abr_345_22","DOIUrl":"https://doi.org/10.4103/abr.abr_345_22","url":null,"abstract":"<p><strong>Background: </strong>Cesarean scar pregnancy (CSP) is a rare long-term complication after cesarean delivery. In clinical practice, its diagnosis and management is very important because it may be associated with serious consequences. Although a wide range of treatment options have been reported, the best treatment is unrecognized yet. This study aimed to evaluate the outcomes of laparoscopy in CSP.</p><p><strong>Materials and methods: </strong>This non-randomized single-arm clinical trial was conducted on 43 CSP patients who were referred to the educational and medical centers of Isfahan University of Medical Science from 2021 to 2022. All the patients were treated by laparoscopy, and during follow-up, the pregnancy test was measured weekly until the test was negative, and an ultrasound was performed 6 months later. Myometrial thickness and occurrence of isthmocele were checked and recorded. Abnormal uterine bleeding or pelvic pain were also evaluated 6 months later.</p><p><strong>Results: </strong>Mean time of surgery was 55.12 ± 10.61 minutes, and the rate of intraoperative bleeding was 62.93 ± 24.00 cc. Four cases had excess intraoperative bleeding: two of them received packed cells, and two led to laparotomy. After surgery, the time to negative pregnancy test was 19.21 ± 5.50 days and 4 (10.3%) patients had isthmocele in follow-up sonography. Human chorionic gonadotropin hormone (BHCG) decreased significantly after surgery (percentage reduction: 97.71 ± 3.80; <i>P</i> value < 0.001) and myometrial thickness increased significantly (percentage increase: 46.94 ± 28.66; <i>P</i> value < 0.001).</p><p><strong>Conclusion: </strong>Laparoscopic excision of a CSP is an effective and feasible technique with the advantage of an immediate myometrial reconstruction.</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"62"},"PeriodicalIF":0.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11478741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic Effects of a Novel tagged Apoptin on Breast Cancer Cell Lines.","authors":"Parisa Lakhshei, Shahrzad Ahangarzadeh, Fatemeh Yarian, Ameneh Koochaki, Bahram Kazemi, Zahra Kiamehr, Elmira Mohammadi, Abbas Alibakhshi","doi":"10.4103/abr.abr_295_23","DOIUrl":"https://doi.org/10.4103/abr.abr_295_23","url":null,"abstract":"<p><strong>Backgrounds: </strong>Apoptin can induce tumor cell-specific apoptosis in a broad range of human tumor cells and is a potential anticancer therapeutic candidate to kill tumor cells.</p><p><strong>Materials and methods: </strong>We designed two structures of apoptin fusion protein, SUMO-PTD4-Apoptin, and PTD4-Apoptin. To express these fusion proteins, <i>E. coli</i> BL21(DE3) was employed. MTT assay, Flow cytometry, and cell cycle analysis were used to investigate the function of proteins on two breast cancer cell lines (MDA-MB-231 and MCF-7) and MCF 10A cell line (as normal cells).</p><p><strong>Results: </strong>Expression of the recombinant SUMO-PTD4-Apoptin and PTD4-Apoptin in <i>E. coli</i> BL21(DE3) was successful. MTT assay results showed that the IC50 was 6.4 µg/ml for SUMO-PTD4-Apoptin in MDA-MB-231 and was 9.3 after 24 h of treatment in MCF-7. The specific cytotoxicity in both cell lines is significant in comparison with MCF-10A, which is used as a normal cell line (IC50 = 29.4). The IC50 for PTD4-Apoptin was 11.07 µg/ml after 24 h of treatment in MDA-MB-231, while the IC50 of PTD4-Apoptin for MCF7 cells was not significantly different from normal cells. The flow cytometry analysis displayed a significant increment in the apoptosis and late apoptosis number in the MDA-MB-231 cells after treatment with SUMO-PTD4-Apoptin and PTD4-Apoptin protein. PTD4-Apoptin and SUMO-PTD4-Apoptin treatment of MDA-MB-231 cells caused a noteworthy increase in the G0-G1 phase and a reduction in the cell population of S and M/G2.</p><p><strong>Conclusion: </strong>This study demonstrates that the fusion of PTD4-Apoptin to SUMO-PTD4-Apoptin could provide an effective method to help enhance the expression and solubility of heterologous Apoptin in <i>E. coli.</i> BL21 (DE3).</p>","PeriodicalId":94292,"journal":{"name":"Advanced biomedical research","volume":"13 ","pages":"46"},"PeriodicalIF":0.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11478704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}