Photodiagnosis and photodynamic therapy最新文献

{"title":"Targeted Photocatalytic Whitening with LED/Laser-Activated Hydrogen Peroxide Gels Containing Nitrogen-Doped TiO₂: A Multi-Study Clinical Analysis of Efficacy, Sensitivity, and Energy Optimization.","authors":"Javier Martín, Milton Carlos Kuga, Andrea Abi Rached, Thais Campos Pereira, Javier Basualdo, Alfredo Von Marttens, Leonardo Díaz, Cristian Bersezio, Eduardo Fernández","doi":"10.1016/j.pdpdt.2025.104713","DOIUrl":"https://doi.org/10.1016/j.pdpdt.2025.104713","url":null,"abstract":"<p><strong>Objective: </strong>This study assessed the clinical efficacy, safety, and energy parameters of 6% and 15% hydrogen peroxide (HP) gels containing nitrogen-doped titanium dioxide (N-TiO₂), activated by dual-wavelength light sources.</p><p><strong>Materials and methods: </strong>A pooled analysis was performed using data from six randomized controlled trials with harmonized protocols. All gels incorporated N-TiO₂ nanoparticles as visible-light-sensitive photocatalysts and were activated using a blue LED (450 ± 10 nm) and an infrared laser (808 ± 10 nm). Standardized dosimetric conditions were maintained (irradiance: 1528-1783 mW/cm²; total energy: 4300-6500 J). Clinical outcomes included spectrophotometric color change (ΔE), shade guide unit change (ΔSGU), and tooth sensitivity measured by visual analog scale (VAS).</p><p><strong>Results: </strong>Bleaching with 6% HP + N-TiO₂ consistently achieved ΔE values >5.0, with low sensitivity scores (VAS < 1.0). The 15% HP formulation showed slightly enhanced whitening (ΔE ≈ 6.5-8.9) with a modest increase in VAS. Long-term follow-up (12-24 months) confirmed sustained color stability and minimal rebound. There was no significant correlation between ΔE and VAS (r = 0.12, p = 0.81), suggesting effective bleaching without increased discomfort.</p><p><strong>Conclusions: </strong>LED/laser-activated gels containing N-TiO₂ offer effective and regulation-compliant whitening with significantly reduced sensitivity. The bleaching effect is attributed to wavelength-specific photocatalysis rather than heat.</p><p><strong>Clinical significance: </strong>This study supports the use of light as a selective photochemical activator in peroxide-based bleaching, challenging generalizations that associate light with thermal sensitivity.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104713"},"PeriodicalIF":0.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pitfalls and other issues with the MTT assay.","authors":"David Kessel","doi":"10.1016/j.pdpdt.2025.104700","DOIUrl":"10.1016/j.pdpdt.2025.104700","url":null,"abstract":"<p><p>While the colorimetric assay commonly referred to as 'MTT' does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent 'cytotoxicity', this is an undefined term and appears not to be necessarily correlated with the object of anti-tumor therapy: tumor eradication. Use of this assay for assessing effects of treatment on viability are therefore unreliable.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104700"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of C-33A cervical carcinoma spheroids on Japanese quail CAM: relevance in assessment of photodiagnosis and photodynamic therapy mediated by hypericin.","authors":"Mariana Máčajová, Veronika Huntošová, Barbora Kundeková, Majlinda Meta, Ivan Čavarga, Boris Bilčík","doi":"10.1016/j.pdpdt.2025.104699","DOIUrl":"https://doi.org/10.1016/j.pdpdt.2025.104699","url":null,"abstract":"<p><p>Cervical cancer is the most common type of cancer in women. The present study aims to establish a reliable model of human cervical cancer microtumours (C-33A) growing on the chorioallantoic membrane (CAM) of quail embryos to test the efficacy of anticancer drugs. Cultrex®, an extracellular matrix, was used as a scaffold for C-33A spheroids. Hypericin, a natural perylene quinone, was used in this study as a model photosensitizer for photodiagnosis and photodynamic therapy (PDT). Fluorescence pharmacokinetics were recorded to verify the robustness of the model. The changes in vessel growth parameters were determined after weak PDT (405 nm, 2 minutes and 285 mW/cm²) with the aim to reduce vascularisation and nutrition of the C-33A spheroids. Vasoconstriction and occlusion of small vessels and capillaries were identified in the CAM. Subsequently, histological examination revealed changes in the chorioallantoic ectoderm of the CAM. An invasion of C-33A cells into the CAM was confirmed. Furthermore, increased expression of growth factor genes was detected by qPCR, indicating angiogenesis of the CAM tissue. In summary, C-33A cells in Cultrex® grown on CAM were shown as a valuable ex ovo 3D spheroid model for photodiagnosis and PDT assays of cervical cancer.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104699"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Halofuginone enhances the anti-tumor effect of ALA-PDT by suppressing NRF2 signaling in cSCC\" [Photodiagnosis and Photodynamic Therapy. 37 (2022) 102572].","authors":"Ting Lv, Jianhua Huang, Minfeng Wu, Hongwei Wang, Qingyu Zeng, Xiuli Wang","doi":"10.1016/j.pdpdt.2025.104657","DOIUrl":"10.1016/j.pdpdt.2025.104657","url":null,"abstract":"","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104657"},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myopic Laser-assisted In-situ Keratomileusis (LASIK) with InnovEyes ablation: a review of literature.","authors":"Yuen Tsing Adeline Ho, Sunny Chi Lik Au","doi":"10.1016/j.pdpdt.2025.104671","DOIUrl":"https://doi.org/10.1016/j.pdpdt.2025.104671","url":null,"abstract":"","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104671"},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory analyses of factors related to 5-aminolevulinic acid-induced intraoperative hypotension during malignant glioma surgery via literature review.","authors":"Kyoichi Tomoto, Akihiko Teshigawara, Tomohiro Aoki, Masahiko Itani, Yuzuru Hasegawa, Yuichi Murayama, Toshihide Tanaka","doi":"10.1016/j.pdpdt.2025.104675","DOIUrl":"https://doi.org/10.1016/j.pdpdt.2025.104675","url":null,"abstract":"<p><strong>Background: </strong>Photodynamic diagnosis using 5-aminolevulinic acid (5ALA) improves glioma resection but has been associated with intraoperative hypotension (IOH), particularly in Japan. Although nitric oxide (NO) production has been suggested as a mechanism, the pathophysiology remains unclear.</p><p><strong>Methods: </strong>We retrospectively analyzed 148 glioma patients who underwent surgery between 2015 and 2024, comparing 5ALA(+) and 5ALA(-) groups in terms of IOH incidence and potential contributing factors, including age, body mass index, antihypertensive drug use, anesthesia duration, and extent of resection. IOH was defined as a mean arterial pressure <60 mmHg after induction. We also assessed phosphorylation of endothelial NO synthase (eNOS) in surgical specimens using immunofluorescence.</p><p><strong>Results: </strong>IOH occurred in 88.9% of the 5ALA(+) group and 82.9% of the 5ALA(-) group (p=0.296). Elderly patients (>60 years old) and those using angiotensin II receptor blockers (ARBs) showed significantly higher IOH incidence in the 5ALA(+) group. Immunofluorescence revealed no detectable phospho-eNOS expression in tumor tissues, regardless of IOH presence.</p><p><strong>Conclusions: </strong>Our findings confirm previously reported risk factors for 5ALA-induced IOH, including older age and ARB use. The lack of eNOS expression in glioma tissues suggests that systemic, rather than tumoral, endothelial responses may mediate IOH via heme-dependent NO synthesis. Further studies using non-tumor tissues or experimental models are needed to elucidate the precise mechanisms and potential interactions between ARBs and 5ALA metabolism.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104675"},"PeriodicalIF":0.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annals of photodynamic therapy: Publish and/or perish?","authors":"David Kessel","doi":"10.1016/j.pdpdt.2024.104398","DOIUrl":"10.1016/j.pdpdt.2024.104398","url":null,"abstract":"","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104398"},"PeriodicalIF":0.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to 18th International Photodynamic Association World Congress.","authors":"Dr. Lothar Lilge","doi":"10.1016/j.pdpdt.2024.104079","DOIUrl":"https://doi.org/10.1016/j.pdpdt.2024.104079","url":null,"abstract":"","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":"88 3","pages":"104079"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigations on correlations between changes of optical tissue properties and NMR relaxation times.","authors":"Maximilian Aumiller, Asmerom Arazar, Ronald Sroka, Olaf Dietrich, Adrian Rühm","doi":"10.1016/j.pdpdt.2024.103968","DOIUrl":"10.1016/j.pdpdt.2024.103968","url":null,"abstract":"<p><strong>Background: </strong>Accurate light dosimetry is a complex remaining challenge in interstitial photodynamic therapy (iPDT) for malignant gliomas. The light dosimetry should ideally be based on the tissue morphology and the individual optical tissue properties of each tissue type in the target region. First investigations are reported on using NMR information to estimate changes of individual optical tissue properties.</p><p><strong>Methods: </strong>Porcine brain tissue and optical tissue phantoms were investigated. To the porcine brain, supplements were added to simulate an edema or high blood content. The tissue phantoms were based on agar, Lipoveneous, ink, blood and gadobutrol (Gd-based MRI contrast agent). The concentrations of phantom ingredients and tissue additives are varied to compare concentration-dependent effects on optical and NMR properties. A 3-tesla whole-body MRI system was used to determine T₁ and T₂ relaxation times. Optical tissue properties, i.e., the spectrally resolved absorption and reduced scattering coefficient, were obtained using a single integrating sphere setup. The observed changes of NMR and optical properties were compared to each other.</p><p><strong>Results: </strong>By adjusting the NMR relaxation times and optical tissue properties of the tissue phantoms to literature values, recipes for human brain tumor, white matter and grey matter tissue phantoms were obtained that mimic these brain tissues simultaneously in both properties. For porcine brain tissue, it was observed that with increasing water concentration in the tissue, both NMR-relaxation times increased, while µ_a decreased and µ_s' increased at 635 nm. The addition of blood to porcine brain samples showed a constant T₁, while T₂ shortened and the absorption coefficient at 635 nm increased.</p><p><strong>Conclusions: </strong>In this investigation, by changing sample contents, notable changes of both NMR relaxation times and optical tissue properties have been observed and their relations examined. The developed dual NMR/optical tissue phantoms can be used in iPDT research, clinical training and demonstrations.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"103968"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139433248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor fluorescence and oxygenation monitoring during photodynamic therapy with chlorin e6 photosensitizer.","authors":"Kanamat Efendiev, Polina Alekseeva, Kirill Linkov, Artem Shiryaev, Tatiana Pisareva, Aida Gilyadova, Igor Reshetov, Arina Voitova, Victor Loschenov","doi":"10.1016/j.pdpdt.2024.103969","DOIUrl":"10.1016/j.pdpdt.2024.103969","url":null,"abstract":"<p><strong>Background: </strong>The study is aimed at developing a method for monitoring photodynamic therapy (PDT) of a tumor using chlorin-type photosensitizers (PSs). Lack of monitoring of chlorin e6 (Cе6) photobleaching, hemoglobin oxygenation and blood flow during light exposure can limit the PDT effectiveness.</p><p><strong>Materials and methods: </strong>Phototheranostics includes spectral-fluorescence diagnostics of Ce6 distribution in the NIR range and PDT with simultaneous assessment of hemoglobin oxygenation and tumor blood flow. Fluorescence diagnostics and PDT were performed using the single laser λ_exc=660 ± 5 nm.</p><p><strong>Results: </strong>Combined spectroscopic PDT monitoring method allowed simultaneous estimation of Ce6 photobleaching, hemoglobin oxygenation and tumor vascular thrombosis during PDT without interrupting the therapeutic light exposure.</p><p><strong>Conclusion: </strong>The developed method of tumor phototheranostics using chlorin-type PSs may make it possible to personalize the duration of therapeutic light exposure during PDT.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"103969"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}