Neda Hakimiha, Negin Barzegar Reyhani, Arian Haddadi, Shabnam Aghayan
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引用次数: 0
Abstract
Objective: Photobiomodulation (PBM), a non-invasive light-based therapy, has gained attention for enhancing osteogenic differentiation in mesenchymal stem cells (MSCs). This systematic review evaluated in vitro evidence regarding PBM effects on the osteogenic differentiation of oral- and adipose-derived human MSCs and to identify effective irradiation parameters.
Methods: A systematic search was conducted across PubMed, Scopus, Web of Science, and Google Scholar for studies published up to November 30, 2024. Of 1104 initially identified articles, 19 met eligibility criteria. They were assessed for methodological quality using the QUIN tool, and Data on cell source, laser parameters, and osteogenic outcomes were extracted.
Results: PBM enhanced proliferation and osteogenic differentiation in MSCs, particularly periodontal ligament stem cells (PDLSCs), dental pulp stem cells (DPSCs), and adipose-derived stem cells (ADSCs). Effective wavelengths, associated with statistically significant osteogenic outcomes, ranged from 660 to 1064 nm, with energy densities of approximately 2-6 J/cm² showing the most consistent osteogenic responses. Key markers such as ALP, RUNX2, and OCN were upregulated, with enhanced mineralization observed. Mechanistically, PBM activated Wnt/β-catenin, BMP/Smad, and PI3K/Akt signalling pathways. QUIN risk-of-bias assessment indicated low risk in most studies, although some showed medium risk due to incomplete reporting.
Conclusion: PBM appears to be a promising adjunctive strategy to promote osteogenic differentiation in vitro using oral- and adipose-derived MSCs. Nevertheless, methodological heterogeneity in study designs and outcome measures highlights the need for standardized protocols. Further translational and clinical studies are warranted to optimize PBM parameters and validate its therapeutic utility in regenerative dentistry and maxillofacial applications.