Neda Hakimiha, Negin Barzegar Reyhani, Arian Haddadi, Shabnam Aghayan
{"title":"光生物调节促进口腔和脂肪来源的间充质干细胞成骨:体外证据的系统综述。","authors":"Neda Hakimiha, Negin Barzegar Reyhani, Arian Haddadi, Shabnam Aghayan","doi":"10.1016/j.pdpdt.2025.105238","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Photobiomodulation (PBM), a non-invasive light-based therapy, has gained attention for enhancing osteogenic differentiation in mesenchymal stem cells (MSCs). This systematic review evaluated in vitro evidence regarding PBM effects on the osteogenic differentiation of oral- and adipose-derived human MSCs and to identify effective irradiation parameters.</p><p><strong>Methods: </strong>A systematic search was conducted across PubMed, Scopus, Web of Science, and Google Scholar for studies published up to November 30, 2024. Of 1104 initially identified articles, 19 met eligibility criteria. They were assessed for methodological quality using the QUIN tool, and Data on cell source, laser parameters, and osteogenic outcomes were extracted.</p><p><strong>Results: </strong>PBM enhanced proliferation and osteogenic differentiation in MSCs, particularly periodontal ligament stem cells (PDLSCs), dental pulp stem cells (DPSCs), and adipose-derived stem cells (ADSCs). Effective wavelengths, associated with statistically significant osteogenic outcomes, ranged from 660 to 1064 nm, with energy densities of approximately 2-6 J/cm² showing the most consistent osteogenic responses. Key markers such as ALP, RUNX2, and OCN were upregulated, with enhanced mineralization observed. Mechanistically, PBM activated Wnt/β-catenin, BMP/Smad, and PI3K/Akt signalling pathways. QUIN risk-of-bias assessment indicated low risk in most studies, although some showed medium risk due to incomplete reporting.</p><p><strong>Conclusion: </strong>PBM appears to be a promising adjunctive strategy to promote osteogenic differentiation in vitro using oral- and adipose-derived MSCs. Nevertheless, methodological heterogeneity in study designs and outcome measures highlights the need for standardized protocols. Further translational and clinical studies are warranted to optimize PBM parameters and validate its therapeutic utility in regenerative dentistry and maxillofacial applications.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"105238"},"PeriodicalIF":2.6000,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Photobiomodulation Enhances Osteogenesis in Oral and Adipose-Derived Mesenchymal Stem cells: A Systematic Review of In Vitro Evidence.\",\"authors\":\"Neda Hakimiha, Negin Barzegar Reyhani, Arian Haddadi, Shabnam Aghayan\",\"doi\":\"10.1016/j.pdpdt.2025.105238\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Photobiomodulation (PBM), a non-invasive light-based therapy, has gained attention for enhancing osteogenic differentiation in mesenchymal stem cells (MSCs). This systematic review evaluated in vitro evidence regarding PBM effects on the osteogenic differentiation of oral- and adipose-derived human MSCs and to identify effective irradiation parameters.</p><p><strong>Methods: </strong>A systematic search was conducted across PubMed, Scopus, Web of Science, and Google Scholar for studies published up to November 30, 2024. Of 1104 initially identified articles, 19 met eligibility criteria. They were assessed for methodological quality using the QUIN tool, and Data on cell source, laser parameters, and osteogenic outcomes were extracted.</p><p><strong>Results: </strong>PBM enhanced proliferation and osteogenic differentiation in MSCs, particularly periodontal ligament stem cells (PDLSCs), dental pulp stem cells (DPSCs), and adipose-derived stem cells (ADSCs). Effective wavelengths, associated with statistically significant osteogenic outcomes, ranged from 660 to 1064 nm, with energy densities of approximately 2-6 J/cm² showing the most consistent osteogenic responses. Key markers such as ALP, RUNX2, and OCN were upregulated, with enhanced mineralization observed. Mechanistically, PBM activated Wnt/β-catenin, BMP/Smad, and PI3K/Akt signalling pathways. QUIN risk-of-bias assessment indicated low risk in most studies, although some showed medium risk due to incomplete reporting.</p><p><strong>Conclusion: </strong>PBM appears to be a promising adjunctive strategy to promote osteogenic differentiation in vitro using oral- and adipose-derived MSCs. Nevertheless, methodological heterogeneity in study designs and outcome measures highlights the need for standardized protocols. Further translational and clinical studies are warranted to optimize PBM parameters and validate its therapeutic utility in regenerative dentistry and maxillofacial applications.</p>\",\"PeriodicalId\":94170,\"journal\":{\"name\":\"Photodiagnosis and photodynamic therapy\",\"volume\":\" \",\"pages\":\"105238\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Photodiagnosis and photodynamic therapy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.pdpdt.2025.105238\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photodiagnosis and photodynamic therapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.pdpdt.2025.105238","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的:光生物调节(PBM)是一种非侵入性的基于光的治疗方法,因其能促进间充质干细胞(MSCs)的成骨分化而受到关注。本系统综述评估了PBM对口服和脂肪来源的人间充质干细胞成骨分化影响的体外证据,并确定了有效的辐照参数。方法:系统检索PubMed、Scopus、Web of Science和b谷歌Scholar,检索截止到2024年11月30日发表的研究。在最初确定的1104篇文章中,有19篇符合资格标准。使用QUIN工具评估它们的方法学质量,并提取细胞来源、激光参数和成骨结果的数据。结果:PBM增强了MSCs的增殖和成骨分化,特别是牙周韧带干细胞(PDLSCs)、牙髓干细胞(DPSCs)和脂肪源性干细胞(ADSCs)。与统计学上显著的成骨结果相关的有效波长范围为660至1064 nm,能量密度约为2-6 J/cm²,显示出最一致的成骨反应。关键标志物如ALP、RUNX2和OCN上调,矿化增强。从机制上讲,PBM激活了Wnt/β-catenin, BMP/Smad和PI3K/Akt信号通路。QUIN风险偏倚评估显示,大多数研究的风险较低,尽管一些研究由于报告不完整而显示为中等风险。结论:PBM似乎是一种很有前途的辅助策略,可以通过口服和脂肪来源的MSCs促进体外成骨分化。然而,研究设计和结果测量的方法异质性突出了标准化方案的必要性。进一步的转化和临床研究需要优化PBM参数,并验证其在再生牙科和颌面外科应用中的治疗效用。
Photobiomodulation Enhances Osteogenesis in Oral and Adipose-Derived Mesenchymal Stem cells: A Systematic Review of In Vitro Evidence.
Objective: Photobiomodulation (PBM), a non-invasive light-based therapy, has gained attention for enhancing osteogenic differentiation in mesenchymal stem cells (MSCs). This systematic review evaluated in vitro evidence regarding PBM effects on the osteogenic differentiation of oral- and adipose-derived human MSCs and to identify effective irradiation parameters.
Methods: A systematic search was conducted across PubMed, Scopus, Web of Science, and Google Scholar for studies published up to November 30, 2024. Of 1104 initially identified articles, 19 met eligibility criteria. They were assessed for methodological quality using the QUIN tool, and Data on cell source, laser parameters, and osteogenic outcomes were extracted.
Results: PBM enhanced proliferation and osteogenic differentiation in MSCs, particularly periodontal ligament stem cells (PDLSCs), dental pulp stem cells (DPSCs), and adipose-derived stem cells (ADSCs). Effective wavelengths, associated with statistically significant osteogenic outcomes, ranged from 660 to 1064 nm, with energy densities of approximately 2-6 J/cm² showing the most consistent osteogenic responses. Key markers such as ALP, RUNX2, and OCN were upregulated, with enhanced mineralization observed. Mechanistically, PBM activated Wnt/β-catenin, BMP/Smad, and PI3K/Akt signalling pathways. QUIN risk-of-bias assessment indicated low risk in most studies, although some showed medium risk due to incomplete reporting.
Conclusion: PBM appears to be a promising adjunctive strategy to promote osteogenic differentiation in vitro using oral- and adipose-derived MSCs. Nevertheless, methodological heterogeneity in study designs and outcome measures highlights the need for standardized protocols. Further translational and clinical studies are warranted to optimize PBM parameters and validate its therapeutic utility in regenerative dentistry and maxillofacial applications.