Experimental gerontology最新文献

{"title":"Associations between the oral microbiome, number of teeth and frailty among American adults: A cross-sectional study from NHANES 2009–2012","authors":"Sixiang Yang ,&nbsp;Yanyun He ,&nbsp;Yuping Ma ,&nbsp;Ruoli Wang ,&nbsp;Yeke Wu ,&nbsp;Wenbin Wu","doi":"10.1016/j.exger.2025.112727","DOIUrl":"10.1016/j.exger.2025.112727","url":null,"abstract":"<div><h3>Background</h3><div>The intricate interrelationship between oral health, the number of teeth, oral microbiota, and frailty remains largely unexplored in clinical research. This study aimed to investigate the interrelationship between oral microbiome, the number of teeth, and frailty.</div></div><div><h3>Methods</h3><div>Data from 4518 participants in NHANES 2009–2012 were analyzed. Frailty was measured using the 48-item Frailty Index (FI). Multivariable logistic regression and restricted cubic spline (RCS) evaluated associations between alpha diversity and frailty. Mediation analysis was used to assess the role of number of teeth. The associations between oral microbiome diveristy and mortality were analyzed by Cox regression. Beta diversity was examined with PCoA and PERMANOVA.</div></div><div><h3>Results</h3><div>The prevalence of frailty was 39.73 %. Univariate analysis showed that alpha diversity indices except for the Simpson index were significantly lower in frailty, and after adjusted for confounders, observed ASVs (adjusted OR: 0.80 [0.73, 0.87], <em>p</em> &lt; 0.001), Faith's PD (adjusted OR: 0.81 [0.74, 0.88], <em>p</em> &lt; 0.001) and Shannon-Weiner index (adjusted OR: 0.88 [0.81, 0.95], <em>p</em> = 0.002) were remained significantly associated with frailty. The reduced number of teeth partially mediated the relationship (for Faith's PD: β_indirect = −0.001 [−0.003, 0.000], <em>p</em> = 0.036, proportion: 8.33 % [0.00 %, 37.50 %]; for Shannon-Weiner index, β_indirect = −0.007 [−0.013, −0.002], <em>p</em> = 0.007, Proportion = 17.07 % [3.39 %, 65.00 %]). Univariable Cox proportional hazard regression showed that all alpha diversity indices were significantly associated with all-cause mortality in frail population, and in multivariable analysis, Shannon-Weiner index (HR: 0.72 [0.55, 0.94], <em>p</em> = 0.017) and Simpson index (HR: 0.71 [0.60, 0.83], <em>p</em> &lt; 0.001) remained statistically significant. PCoA showed that beta diversity was also significantly associated with frailty.</div></div><div><h3>Conclusion</h3><div>Lower oral microbiome diversity is associated with higher frailty and mortality. The number of teeth partially mediates this link, emphasizing the importance of oral health in mitigating frailty and promoting healthy aging.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"203 ","pages":"Article 112727"},"PeriodicalIF":3.9,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into EIF6 as a target of Apigenin in alleviating chondrocyte senescence","authors":"Erliang Li ,&nbsp;Hui Yu ,&nbsp;Xin Xu ,&nbsp;Min Wang ,&nbsp;Mingyi Yang ,&nbsp;Zhi Yang ,&nbsp;Peng Xu","doi":"10.1016/j.exger.2025.112725","DOIUrl":"10.1016/j.exger.2025.112725","url":null,"abstract":"<div><h3>Purpose</h3><div>Apigenin, a flavonoid found in edible plants, has demonstrated therapeutic potential in various diseases, but its role in knee osteoarthritis (KOA) remains unclear. This study aimed to identify the potential targets and mechanisms of Apigenin in KOA.</div></div><div><h3>Methods</h3><div>Network pharmacology analysis identified 80 targets of Apigenin, of which 48 overlapped with KOA-related targets. Summary-data-based Mendelian randomization (SMR) analysis and molecular docking were utilized to explore key target genes. Single-cell RNA sequencing data from human cartilage tissue and in vitro studies using SW1353 cells treated with 3 % hydrogen peroxide (H2O2) were analyzed to validate findings.</div></div><div><h3>Results</h3><div>SMR analysis identified EIF6 as a potential target of Apigenin in KOA, negatively associated with disease progression. Molecular docking revealed strong binding affinity between Apigenin and EIF6. Single-cell analysis suggested downregulation of EIF6 may contribute to chondrocyte senescence. In vitro, Apigenin (20 μM) reversed H2O2-induced senescence and increased EIF6 expression in SW1353 cells, improving cell viability.</div></div><div><h3>Conclusion</h3><div>Apigenin upregulates EIF6 expression and mitigates H2O2-induced chondrocyte senescence, highlighting its potential as a therapeutic agent for KOA. These findings provide insights into the nutritional health benefits of Apigenin and its implications for KOA treatment.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"203 ","pages":"Article 112725"},"PeriodicalIF":3.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obesity classified by anthropometric parameters was associated with mitochondrial bioenergetics impairment of peripheral blood mononuclear cells in the elderly population","authors":"Tanawat Attachaipanich ,&nbsp;Sirawit Sriwichaiin ,&nbsp;Nattayaporn Apaijai ,&nbsp;Thanaphat Thanyaratsarun ,&nbsp;Nisakron Thongmung ,&nbsp;Prin Vathesatogkit ,&nbsp;Piyamitr Sritara ,&nbsp;Nipon Chattipakorn ,&nbsp;Chagriya Kitiyakara ,&nbsp;Siriporn C. Chattipakorn","doi":"10.1016/j.exger.2025.112724","DOIUrl":"10.1016/j.exger.2025.112724","url":null,"abstract":"<div><div>Waist circumference (WC), waist-to-height ratio (WHtR), and waist-to-hip ratio (WHR), even in individuals who have a normal body mass index (BMI), are correlated with cardiovascular events. The aim of this study is to establish the association between obesity and mitochondrial bioenergetics in peripheral blood mononuclear cells (PBMCs). The study included 1584 subjects from the Electricity Generating Authority of Thailand (EGAT) cohort. The mean age of participants in this study was 68.4 years. There was 24.2 % diabetes mellitus (DM) with a mean HbA1c level of 6.8. WC, WHR, and WHtR were associated with decreased basal, maximal respiration, spare respiratory capacity (SRC), and ATP production, whereas BMI was only associated with reduced maximal respiration and SRC. We further stratified the participants into four groups based on obesity classified by WHR and DM status: Non-DM/Non-obese (<em>n</em> = 468), Non-DM/Obese (<em>n</em> = 733), DM/Non-obese (<em>n</em> = 84), and DM/Obese (<em>n</em> = 299). Both obesity and DM were associated with mitochondrial bioenergetic impairment and increased mitochondrial oxidative stress. Interestingly, there was no difference in mitochondrial bioenergetics impairment between non-DM/Obese and DM participants. Our study demonstrated that WC, WHR, and WHtR better reflected underlying mitochondrial dysfunction in PBMCs compared to BMI. Furthermore, obesity was associated with mitochondrial dysfunction to the same degree as DM.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112724"},"PeriodicalIF":3.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a risk prediction model for frailty in older nappers","authors":"Lijing Chen ,&nbsp;Jiaxian Wang ,&nbsp;Ning Liu ,&nbsp;Li Geng ,&nbsp;Jiahui Li ,&nbsp;Aifang He ,&nbsp;Xuemei Shi ,&nbsp;Yi Li","doi":"10.1016/j.exger.2025.112723","DOIUrl":"10.1016/j.exger.2025.112723","url":null,"abstract":"<div><h3>Background</h3><div>Frailty among older adults has received widespread attention from society, especially among nappers. The objective of this study was to develop a frailty prediction model for nappers.</div></div><div><h3>Methods</h3><div>The data source was the China Health and Retirement Longitudinal Study, with a cohort of 1830 older nappers. We used the least absolute shrinkage and selection operator to screen the best predictors from multiple factors, logistic regression analysis to explore the best predictors of frailty in older nappers, and nomogram to establish a prediction model. A calibration curve was used to evaluate the precision of the model, and the predictive performance was assessed by analyzing the area under the characteristic and decision curves.</div></div><div><h3>Results</h3><div>The prevalence of frailty among older nappers was 28.9 % (528/1830). Chronic diseases, physical activity, sleep quality, pain, fatigue, depression, nap duration, and nighttime sleep duration were the best predictive factors for frailty in older nappers. The area under the curve (AUC) in the training set was 0.751 (95 % confidence interval [CI] = 0.724–0.779) with a specificity of 0.662 and sensitivity of 0.711. The AUC in the validation set was 0.781 (95 % CI = 0.749–0.812) with a specificity of 0.730 and sensitivity of 0.714. The Hosmer–Lemeshow test values were both <em>p</em> &gt; 0.05. The nomogram model showed good concordance and accuracy.</div></div><div><h3>Conclusion</h3><div>We constructed a nomogram that serves as a valuable and convenient instrument for assessing the prevalence of frailty among older nappers.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112723"},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of sarcopenia in Asian older adults: A comparison of nine diagnostic criteria across different regions","authors":"Wei-Cheng Chiu ,&nbsp;Tung-Wei Kao ,&nbsp;Tao-Chun Peng","doi":"10.1016/j.exger.2025.112721","DOIUrl":"10.1016/j.exger.2025.112721","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to compare sarcopenia prevalence in older adults using nine diagnostic criteria from different regions to assess how these guidelines influence prevalence rates within the same population. Additionally, we analyzed variations across subgroups to identify factors contributing to prevalence differences.</div></div><div><h3>Methods</h3><div>A total of 1760 participants aged 65–99 were enrolled. Bioelectrical impedance analysis was used to assess muscle mass, while muscle strength and physical performance were evaluated using grip strength, gait speed, and the repeated chair stands test. Sarcopenia prevalence was determined based on definitions provided by ESPEN (European Society for Clinical Nutrition and Metabolism), EWGSOP (European Working Group on Sarcopenia in Older People), IWGS (International Working Group on Sarcopenia), SCWD (Society for Sarcopenia, Cachexia, and Wasting Disorders), AWGS (Asian Working Group for Sarcopenia), FNIH (Foundation for the National Institutes of Health), and SDOC (Sarcopenia Definitions and Outcomes Consortium). Additionally, prevalence rates were assessed across subgroups based on age, sex, and BMI categories.</div></div><div><h3>Results</h3><div>Sarcopenia prevalence varied from 4.8 % (<em>n</em> = 79), based on the FNIH criteria, to 16.1 % (<em>n</em> = 261), according to the EWGSOP criteria. Among females, higher prevalence rates were observed using the ESPEN, AWGS, and EWGSOP2 criteria, while the FNIH criteria indicated a higher prevalence in males. Prevalence increased with age, especially in those aged 85 and older. Lower BMI was associated with higher sarcopenia prevalence according to most criteria, except the FNIH and ESPEN.</div></div><div><h3>Conclusion</h3><div>The notable variability in sarcopenia prevalence across different diagnostic criteria highlights the need for population-specific guidelines. Refining diagnostic criteria to address demographic variations could enhance the accuracy and applicability of sarcopenia assessments. Future studies should aim to further tailor diagnostic approaches and interventions to meet the needs of diverse populations.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112721"},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aerobic exercise modulates aortic chondrogenesis and calcification via 5-methoxytryptophan and P38MAPK in atherosclerotic rats","authors":"Liang-liang You ,&nbsp;Xiao-bing Luo ,&nbsp;Wen-qi Zhou ,&nbsp;Rui-chi Zhang ,&nbsp;Zhong-hao Li ,&nbsp;Jia-xin Xu ,&nbsp;Jing Ran ,&nbsp;Jie Xu","doi":"10.1016/j.exger.2025.112722","DOIUrl":"10.1016/j.exger.2025.112722","url":null,"abstract":"<div><h3>Background</h3><div>5-Methoxytryptophan (5-MTP), a new endothelial factor with vasoprotective and anti-inflammatory effects, reduces aortic chondrogenesis and calcification during atherosclerosis. The aim of this study was to investigate the effects of aerobic exercise on aortic chondrogenesis and calcification during atherosclerosis in rats. To investigate the effect of aerobic exercise on the expression of 5-MTP/P38 MAPK signaling pathway. To lay a theoretical foundation for the therapeutic effect of exercise in rat atherosclerosis model.</div></div><div><h3>Methods</h3><div>Establishment of a rat model of atherosclerosis using a high-fat diet combined with intraperitoneal injection of vitamin D3 (VD3). The aerobic exercise group underwent moderate-intensity aerobic exercise on an exercise treadmill for 8 weeks, while the atherosclerosis model group and the control group did not exercise. After exercise, blood and aortic samples were collected from all rats to evaluate the levels of serum triglyceride (TG), cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL<img>C), aortic chondrogenesis, calcification, 5-MTP level, collagen II, P38MAPK, pp38 MAPK, and IL-6 protein content.</div></div><div><h3>Results</h3><div>(1)8 weeks of aerobic exercise significantly reduced aortic chondrogenesis, area of calcification, serum LDL-C, TC levels, atherosclerotic index and serum IL-6 levels in rats (<em>p</em> &lt; 0.01), and lowered TG levels (<em>p</em> &lt; 0.05);(2)8 weeks of aerobic exercise significantly increased aortic 5-MTP levels (<em>p</em> &lt; 0.01) and decreased the content of aortic pp38MAPK, collagen II and IL-6 proteins significantly (<em>p</em> &lt; 0.01). The pp38MAPK/P38MAPK ratio was also decreased (p &lt; 0.01).</div></div><div><h3>Conclusion</h3><div>8 weeks of aerobic exercise training improved dyslipidemia and reduced aortic chondrogenesis and calcification formation in AS rats. The mechanism may be related to increasing aortic 5-MTP levels and inhibiting the P38MAPK/ IL-6 signaling pathway.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112722"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of green tea catechins and exercise on age-related muscle atrophy and satellite cell functions in a mouse model of sarcopenia","authors":"Ryoma Matsuzaki ,&nbsp;Teruki Matsuoka ,&nbsp;Kazuki Nakanishi ,&nbsp;Akira Tani ,&nbsp;Shogo Kakimoto ,&nbsp;Yuki Kato ,&nbsp;Takuya Kawatani ,&nbsp;Sae Nakagawa ,&nbsp;Yoshitake Baba ,&nbsp;Makoto Kobayashi ,&nbsp;Takanobu Takihara ,&nbsp;Harutoshi Sakakima","doi":"10.1016/j.exger.2025.112720","DOIUrl":"10.1016/j.exger.2025.112720","url":null,"abstract":"<div><div>Sarcopenia negatively affects the quality of life and health of older individuals. Physical exercise is a standard treatment for sarcopenia. Recently, the potential benefits of green tea catechins (GTCs) in sarcopenia have gained considerable attention. In this study, we investigated the effects of a combination of GTCs and physical exercise on the symptoms and pathologies of sarcopenia using male senescence-accelerated mouse prone 8 (SAMP8). These mice were divided into four groups: control, GTCs, exercise (Ex), and GTCs + Ex. GTC-fed mice were fed a diet containing 0.33 % GTCs. The mice were subjected to exercise training (voluntary wheel running) for 12 weeks, from 5 to 8 months of age, and grip strength and gastrocnemius muscle alterations were investigated. SAMP8 mice exhibited symptoms and pathologies of sarcopenia, including loss of muscle mass, decreased grip strength, reduced mitochondrial capacity, increased oxidative stress, reduced number of satellite cells, and an increased ratio of 5-bromo-2′-deoxyuridine (BrdU)-positive nuclei located within the muscle cells in the aged muscle. The GTCs and/or Ex groups showed improved symptoms and pathologies of sarcopenia. In addition, the GTCs + Ex group exhibited enhanced mitochondrial capacity, myogenic differentiation, and maturation in aged skeletal muscle than that observed in the GTCs group. Our findings suggested that GTCs and/or Ex are effective in ameliorating several age-related changes in muscle morphology and function. Notably, GTCs intake, together with habitual exercise, may enhance the beneficial effects on the symptoms and pathologies of sarcopenia in aged muscle.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112720"},"PeriodicalIF":3.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Young bone marrow transplantation delays bone aging in old mice","authors":"Lina Abu-Nada ,&nbsp;Younan Liu ,&nbsp;Faez Saleh Al-Hamed ,&nbsp;Bouchra Ouliass ,&nbsp;Magali Millecamps ,&nbsp;Simon D. Tran ,&nbsp;Guylaine Ferland ,&nbsp;Vahab D. Soleimani ,&nbsp;Faleh Tamimi Marino ,&nbsp;Monzur Murshed","doi":"10.1016/j.exger.2025.112704","DOIUrl":"10.1016/j.exger.2025.112704","url":null,"abstract":"<div><div>Recent discoveries have shown that systemic manipulations, such as parabiosis, blood exchange, and young plasma transfer, can counteract many hallmarks of aging. This rejuvenation effect has been attributed to circulatory factors produced by cells from both hematopoietic and non-hematopoietic lineages. However, the specific involvement of bone marrow (BM) or hematopoietic cells in producing such factors and their effects on aging is still unclear. We developed a model of aged mice with transplanted young or old BM cells and assessed the impact on the aging process, specifically on energy metabolism and bone remodeling parameters. The donor BM cell engraftment in the aged mice was confirmed by flow cytometry using a transplanted cell-specific marker (green fluorescent protein). Energy metabolism was assessed using Oxymax indirect calorimetry system after 3 months of transplantation. Tibiae and L3-L4 vertebrae were analyzed using micro-CT, a three-point bending test and bone histomorphometry. Moreover, bone marrow proteome was assessed using proteomics, and blood serum/plasma was collected and analyzed using the Luminex assay. Our results showed that while the effect on energy metabolism was insignificant, rejuvenating the BM through young bone marrow transplantation reversed age-associated low bone mass traits in old mice. Specifically, young bone marrow transplantation improved bone trabecular microarchitecture both in tibiae and vertebrae of old mice and increased the number of osteoblasts and osteoclasts compared to old bone marrow transplantation. In conclusion, young bone marrow cells may represent a future therapeutic strategy for age-related diseases such as osteoporosis. The findings of this study provide important insights into our understanding of aging.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112704"},"PeriodicalIF":3.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhythms and shifts of chemokines and cytokines interplay in a decade lifespan: The longitudinal community-based Bambuí health and aging study","authors":"Joaquim Pedro Brito-de-Sousa ,&nbsp;Maria Luiza Lima-Silva ,&nbsp;Ismael Artur Costa-Rocha ,&nbsp;Ana Carolina Campi-Azevedo ,&nbsp;Juliana Vaz de Melo Mambrini ,&nbsp;Ana Maria Caetano Faria ,&nbsp;Maria Fernanda Lima-Costa ,&nbsp;Sérgio Viana Peixoto ,&nbsp;Andréa Teixeira-Carvalho ,&nbsp;Karen Cecília Lima Torres ,&nbsp;Olindo Assis Martins-Filho","doi":"10.1016/j.exger.2025.112700","DOIUrl":"10.1016/j.exger.2025.112700","url":null,"abstract":"<div><div>Aging is associated with several physiological changes, including a remarkable remodeling of the immune system. Herein, the rhythms and shifts in serum immune mediators were characterized in a decade lifespan as a longitudinal community-based prospective investigation from Bambuí Health and Aging Study. The study population included paired samples from 713 subjects survivors from the original BHAS cohort and at 10-years Follow-up, categorized into 5-years age range intervals (60-64^Yrs towards 90 + ^Yrs). Quantification of soluble mediators were carried out by Cytometric Bead Array. The results demonstrated a rhythmic increase in serum immune mediators, especially CXCL9, CXCL10, IL-1β, IL-6 and TNF following the aging process, particularly at age intervals 70-74^Yrs and 85-89^Yrs. More prominent fold change magnitudes were observed for TNF (27.64×), CXCL9 (2.40×), IL-1β (2.20×), IL-6 (1.47×), and CXCL10 (1.26×). On the other hand, analysis of integrative networks showed a waning in the correlation numbers between immune mediators in a decade lifespan and a shift of connectivity from chemokines at Enrollment towards cytokines at 10-years Follow-up. Cross-correlation approaches revealed that CXCL9, CXCL10, IL-1β, IL-6, and IL-10 were placed in the innermost position, underscoring the higher contribution of these mediators along aging. Overall, these findings re-emphasize the impact of aging in the dynamic profile of serum immune mediators, highlighting the shift of selective mediators and their rhythmic signatures across chronological aging.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112700"},"PeriodicalIF":3.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The causal relationship of inflammation-related factors with osteoporosis: A Mendelian Randomization Analysis","authors":"Xinyue Yang ,&nbsp;Rui Xiao ,&nbsp;Beizhong Liu ,&nbsp;Bo Xie ,&nbsp;Zhao Yang","doi":"10.1016/j.exger.2025.112715","DOIUrl":"10.1016/j.exger.2025.112715","url":null,"abstract":"<div><h3>Background</h3><div>We used Mendelian randomization (MR) approach to examine whether genetically determined inflammation-related risk factors play a role in the onset of osteoporosis (OP) in the European population.</div></div><div><h3>Methods</h3><div>Genome-wide association studies (GWASs) summary statistics of estimated bone mineral density (eBMD) obtained from the public database GEnetic Factors for OSteoporosis Consortium (GEFOS) including 142,487 European people. For exposures, we utilized GWAS data of 9 risk factors including diseases chronic kidney disease (CKD) (41,395 cases and 439,303 controls), type 2 diabetes (T2D) (88,427 cases and 566,778 controls), Alzheimer's disease (AD) (71,880 cases, 383,378 controls) and major depression disorder (MDD) (9240 cases and 9519 controls) and lifestyle behaviors are from different consortiums. Inverse variance weighted (IVW) analysis was principal method in this study and random effect model was applied; MR-Egger method and weighted median method were also performed for reliable results. Cochran's Q test and MR-Egger regression were used to detect heterogeneity and pleiotropy and leave-one-out analysis was performed to find out whether there are influential SNPs.</div></div><div><h3>Results</h3><div>We found that T2D (<em>IVW: β =</em> <em>0.05, P</em> <em>=</em> <em>0.0014</em>), FI (<em>IVW: β = −0.22, P</em> <em>&lt;</em> <em>0.001</em>), CKD (<em>IVW: β =</em> <em>0.02, P</em> <em>=</em> <em>0.009</em>), ALZ (<em>IVW: β =</em> <em>0.06, P</em> <em>=</em> <em>0.005</em>), Coffee consumption (<em>IVW: β = 0.11, P</em> <em>=</em> <em>0.003</em>) were causally associated with OP (<span><math><mi>P</mi><mo>&lt;</mo><mn>0.006</mn><mspace></mspace></math></span>after Bonferroni correction).</div></div><div><h3>Conclusions</h3><div>Our study revealed that T2D, FI, CKD, ALZ and coffee consumption are causally associated with OP. Future interventions targeting factors above could provide new clinical strategies for the personalized prevention and treatment of osteoporosis.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"202 ","pages":"Article 112715"},"PeriodicalIF":3.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}