Experimental gerontology最新文献

{"title":"Examining the impact of internet-enabled nursing practices, guided by specialist nurses on patients with hypertension and their caregivers","authors":"Xiao-Juan He ,&nbsp;Xin-Yu Yi ,&nbsp;Na Wei","doi":"10.1016/j.exger.2024.112606","DOIUrl":"10.1016/j.exger.2024.112606","url":null,"abstract":"<div><h3>Purpose</h3><div>To aim of this study is to assess the impact of an internet-enabled nursing model, led by specialized nurses within a framework of multidisciplinary collaboration, on both, patients diagnosed with hypertension, and their respective caregivers.</div></div><div><h3>Methods</h3><div>A total of 158 patients with hypertension, along with their corresponding caregivers, were meticulously selected and paired. By using a random number table method, participants were allocated into either a control group or an observation group. The control group received conventional health education, blood pressure monitoring, and routine telephone follow-ups administered by designated nurses. Conversely, patients in the observation group underwent treatment within an internet-enabled nursing model, led by specialist nurses within a multidisciplinary collaborative framework. Parameters including systolic and diastolic blood pressure readings of the patients, as well as their scores in compliance with the hypertension treatment, quality of life, and caregiving proficiency of family members, which were meticulously documented prior to intervention (T₀), as well as at 3- and 6-month intervals post-intervention (T₁ and T₂).</div></div><div><h3>Results</h3><div>Statistically significant differences were observed in both systolic and diastolic blood pressure levels among patients, as well as in their scores reflecting compliance with hypertension treatment, quality of life, and caregiving proficiency of family members, when comparing pre- and post-intervention periods within each group, across groups, and within the interaction effect (<em>p</em> &lt; 0.05). Also, there were statistically significant differences in the aforementioned parameters between the two groups at adjacent time points (<em>p</em> &lt; 0.05). Specifically, patients within the observation group exhibited notable reductions in systolic and diastolic blood pressure levels at both T₁ and T₂, alongside heightened scores indicative of enhanced compliance with hypertension treatment, enhanced quality of life, and increased caregiving proficiency among family members, when compared to patients within the control group (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The implementation of an internet-enabled nursing model, overseen by specialized nurses within a framework of multidisciplinary collaboration, demonstrates superior efficacy in preserving the stability of blood pressure among patients with hypertension. This model significantly enhances patient compliance with treatment regimens, enhances their overall quality of life, and fosters heightened caregiving proficiency among their respective caregivers.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112606"},"PeriodicalIF":3.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of tamoxifen as an adjuvant therapy for breast cancer on apolipoproteins and lipoprotein(a) concentrations in women: A meta-analysis of randomized controlled trials","authors":"Bohua Zhang ,&nbsp;Yafang Huang ,&nbsp;Jingjing Zhang ,&nbsp;Wenbo Fu ,&nbsp;Kousalya Prabahar ,&nbsp;Benjamin Hernández-Wolters ,&nbsp;Hua Hu ,&nbsp;Fei Hao","doi":"10.1016/j.exger.2024.112587","DOIUrl":"10.1016/j.exger.2024.112587","url":null,"abstract":"<div><h3>Background and aim</h3><div>Tamoxifen has been used in the management of breast cancer. The available evidence on the effect of tamoxifen on lipoprotein(a) and apolipoproteins is controversial. Hence, this meta-analysis of randomized controlled trials (RCTs) was conducted to increase the quality of evidence on the effect of tamoxifen on lipoprotein(a) and apolipoproteins.</div></div><div><h3>Methods</h3><div>Eligible RCTs published up to September 2023 were carefully selected following a comprehensive search. Thereafter, a meta-analysis was conducted using a random-effects model and the results were presented as the weighted mean difference (WMD) with a 95 % confidence interval (CI).</div></div><div><h3>Results</h3><div>The results from the random-effects model indicated a rise in ApoA-I (WMD: 16.24 mg/dL, 95 % CI: 5.35, 27.12, <em>P</em> = 0.003), and a decrease in ApoB (WMD: −9.37 mg/dL, 95 % CI: −15.16, −3.59, <em>P</em> = 0.001) and lipoprotein(a) (WMD: −3.24 mg/dL, 95 % CI: −5.66, −0.83, <em>P</em> &lt; 0.001) concentrations following tamoxifen administration in women. Furthermore, a more pronounced decrease in ApoB (WMD: −12.86 mg/dL, 95 % CI: −19.78, −5.93, <em>P</em> &lt; 0.001) and elevation in ApoA-1 levels (WMD: 51.97 mg/dL, 95 % CI: 45.89, 58.05, P &lt; 0.001) were identified in a single study on patients with breast cancer.</div></div><div><h3>Conclusion</h3><div>The current meta-analysis demonstrated an increase of ApoA-I and a decrease of ApoB and lipoprotein(a) levels after treatment with tamoxifen in women.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112587"},"PeriodicalIF":3.9,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the effective age for dietary restriction and the key metabolites involved","authors":"Jazween Loo,&nbsp;Geetha Gunasekaran,&nbsp;Jen Kit Tan,&nbsp;Jo Aan Goon","doi":"10.1016/j.exger.2024.112601","DOIUrl":"10.1016/j.exger.2024.112601","url":null,"abstract":"<div><div>Dietary restriction (DR) extends lifespan in various species, but its effect at different ages, especially when started later, is unclear. This study used <em>Caenorhabditis elegans</em> to explore the impact of DR at different ages. Worms were divided into control and DR groups, with daily survival monitored. To confirm the occurrence of DR, the expression of DR-sensitive genes namely <em>acdh-1</em>, <em>pyk-1</em>, <em>pck-2</em> and <em>cts-1</em> were determined using RT-qPCR. Liquid chromatography mass spectrometry (LC-MS) was employed to observe the changes in metabolites affected by DR. The results indicated that young worms subjected to mild DR displayed the longest lifespan, highlighting the effectiveness of initiating DR at a young age. Increased expression of <em>acdh-1</em> and <em>pck-2</em> suggests activation of beta-oxidation and gluconeogenesis, while decreased <em>cts-1</em> expression indicates a reduced citric acid cycle, further supporting the observed effects of DR in these worms. Metabolomic results indicated that DR decreased the activity of mechanistic Target of Rapamycin (mTOR) and the synthesis of amino acids namely leucine, tyrosine and tryptophan to conserve energy for cell repair and survival. DR also decreased levels of <em>N</em>-acetyl-L-methionine and S-adenosyl-methionine (SAM) in methionine metabolism, thereby promoting autophagy, reducing inflammation, and facilitating the removal of damaged cells and proteins. In conclusion, initiating dietary restriction early in life extends the lifespan by modulating amino acid metabolism and enhancing the autophagy pathway, thereby maintaining cellular wellbeing.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112601"},"PeriodicalIF":3.9,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development of intrinsic capacity measures for longitudinal research: The Longitudinal Aging Study Amsterdam","authors":"Yuwei Qi ,&nbsp;Laura A. Schaap ,&nbsp;Benjamin D. Schalet ,&nbsp;Emiel O. Hoogendijk ,&nbsp;Dorly J.H. Deeg ,&nbsp;Marjolein Visser ,&nbsp;Kaisa Koivunen ,&nbsp;Martijn Huisman ,&nbsp;Natasja M. van Schoor","doi":"10.1016/j.exger.2024.112599","DOIUrl":"10.1016/j.exger.2024.112599","url":null,"abstract":"<div><h3>Background</h3><div>The World Health Organization has introduced the construct of intrinsic capacity (IC) as an important component of healthy ageing and overall well-being in older adults The present study aimed to develop domain-specific and composite IC scores and to validate these scores by examining their longitudinal relation with functioning.</div></div><div><h3>Methods</h3><div>We used prospective data on participants aged 57 to over 90 years, with a 10-year follow-up, from the Longitudinal Aging Study Amsterdam, an ongoing cohort study of older Dutch men and women Using a formative, stepwise approach, we identified indicators across the different domains of IC, i.e. vitality, sensory, cognition, psychology, and locomotion, using a combination of unidimensional factor analyses and Partial Least Squares Structural Equation Modelling (PLS-SEM). Next, domain-specific and composite IC scores were generated, and the construct validity (score across age groups) and criterion validity (relationship with change in functional limitations) were assessed.</div></div><div><h3>Results</h3><div>The multiple unidimensional factor analyses and PLS-SEM identified a total of 18 indicators, covering the five domains of IC. The mean composite IC score was 70.9 (SD = 0.9) in men and 69.7 (0.8) in women. The domain-specific and composite IC scores all showed good construct validity, with known-group validation results indicating age-related declines. A higher composite IC score was associated with less functional limitations over time (B = 0.20, 95%CI [0.19, 0.22]).</div></div><div><h3>Conclusion</h3><div>The developed domain-specific IC scores and the composite IC score effectively discriminated age-related declines in IC. Additionally, the composite IC score was longitudinally associated with functional limitations. By creating this comprehensive and reliable tool for tracking IC, we aim to provide valuable insights into the dynamics of ageing and support more effective strategies for promoting health and well-being throughout later life. These scores establish a foundation for future research to track longitudinal changes across various IC domains and relate these changes to key age-related outcomes.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112599"},"PeriodicalIF":3.9,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142376477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between nighttime sleep duration and quality with low back pain in middle-aged and older Chinese adults","authors":"Jiju Yang ,&nbsp;Cong Liu ,&nbsp;Guanwei Wu ,&nbsp;Sihao Zhao","doi":"10.1016/j.exger.2024.112602","DOIUrl":"10.1016/j.exger.2024.112602","url":null,"abstract":"<div><h3>Background</h3><div>This study aims to investigate the association between sleep duration, sleep quality, and the risk of low back pain (LBP) among middle-aged and elderly individuals in China.</div></div><div><h3>Methods</h3><div>Utilizing data from the China Health and Retirement Longitudinal Study spanning 2011 to 2020, we categorized sleep duration into long, medium, and short categories, and sleep quality into good, fair, and poor levels. LBP status was determined based on self-reported information by participants. Multivariable logistic regression analysis was employed to assess the relationship between sleep duration, sleep quality, with the risk of LBP.</div></div><div><h3>Results</h3><div>A total of 6750 individuals were included in the longitudinal study. After 9 years of follow-up, the prevalence of LBP was 25.69 %. In the fully adjusted model, compared to individuals with medium sleep duration, those with short sleep duration had a 28 % increased risk of developing LBP (OR = 1.28, 95%CI: 1.12, 1.46). Additionally, when compared to individuals with good sleep quality, those with fair and poor sleep quality had a 35 % (OR = 1.35, 95%CI: 1.19, 1.54) and 33 % (OR = 1.33, 95%CI: 1.12, 1.58) increased risk of LBP, respectively. Joint analysis of both factors revealed that individuals with poor sleep quality and short sleep duration had the highest risk of LBP.</div></div><div><h3>Conclusion</h3><div>Among middle-aged and elderly individuals in China, short sleep duration and poor sleep quality were associated with an increased risk of LBP. Future studies should further explore the mechanisms underlying this association and validate the findings through randomized controlled trials.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112602"},"PeriodicalIF":3.9,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142368017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frailty increases depression risk independently of cognitive decline: Insights from Mendelian randomization and cross-sectional analysis","authors":"Wenjie Li ,&nbsp;Qi Tian ,&nbsp;Jingxi Duan ,&nbsp;Xintong Liu ,&nbsp;Jianwei Shou ,&nbsp;Ting Tang ,&nbsp;Weihua Yu ,&nbsp;Yang Lü","doi":"10.1016/j.exger.2024.112603","DOIUrl":"10.1016/j.exger.2024.112603","url":null,"abstract":"<div><h3>Background</h3><div>Frailty, cognitive decline, and depression are common syndromes among the elderly and are closely interconnected. However, it is still unclear whether the impact of frailty on depression depends on the role of cognitive decline.</div></div><div><h3>Method</h3><div>We conducted the Mendelian randomization (MR) analysis based on the instrumental variables (IVs) from the genome-wide association study (GWAS) databases, and we also performed a cross-sectional study consisting of 1362 older adults aged ≥65 for validation.</div></div><div><h3>Results</h3><div>The results of the multivariable MR analysis showed that frailty significantly increased the risk of depression, even after controlling for the influence of cognitive performance. Conversely, after controlling for frailty, the effect of cognitive performance on depression risk was noticeably reduced. In the cross-sectional study, frailty mediated 24.04 % of the relationship between cognition and depression, and cognition mediated 7.63 % of the relationship between frailty and depression.</div></div><div><h3>Conclusions</h3><div>We provide evidence that frailty could increase depression risk independently of cognitive decline. Further research with a larger sample size is necessary.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112603"},"PeriodicalIF":3.9,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142376476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNAs as commonly expressed biomarkers for sarcopenia and frailty: A systematic review","authors":"Hyung Eun Shin ,&nbsp;Jae Young Jang ,&nbsp;Heeeun Jung ,&nbsp;Chang Won Won ,&nbsp;Miji Kim","doi":"10.1016/j.exger.2024.112600","DOIUrl":"10.1016/j.exger.2024.112600","url":null,"abstract":"<div><h3>Background</h3><div>Coexistent sarcopenia and frailty is more strongly associated with adverse health outcomes than each condition alone. As the importance of coexistent sarcopenia and frailty increases, exploring their underlying mechanisms is warranted. Recently, noncoding ribonucleic acids (RNAs) have been suggested as potential biomarkers of sarcopenia and frailty. This systematic review aimed to summarize noncoding RNAs commonly expressed in sarcopenia and frailty, and to search the predicted target genes and biological pathways of them.</div></div><div><h3>Methods</h3><div>We systematically searched the literatures on PubMed, Embase, Cochrane Library, Web of Science, and Scopus for literature published till November 15, 2023. A total of 7,202 literatures were initially retrieved. After de-duplication, 34 studies (26 sarcopenia-related and 8 frailty-related) were full-text reviewed, and 15 studies (11 sarcopenia-related and 4 frailty-related) were finally included.</div></div><div><h3>Results</h3><div>miR-29a-3p, miR-29b-3p, and miR-328 were identified as commonly expressed in same direction in sarcopenia and frailty. These microRNAs (miRNAs), identified in the literature search using PubMed, modulate transforming growth factor-β signaling via extracellular matrix components and calcineurin/nuclear factor of activated T cells 3 signaling via sarcoplasmic/endoplasmic reticulum Ca²⁺ ATPase 2a, which are involved in regulating skeletal muscle fibrosis and the growth of slow-twitch muscle fibers, respectively. miR-155-5p, miR-486, and miR-23a-3p were also commonly expressed in two conditions, although in different or conflicting directions.</div></div><div><h3>Conclusion</h3><div>In this systematic review, we highlight the potential of shared miRNAs that exhibit consistent expression patterns as biomarkers for the early diagnosis and progression assessment of both sarcopenia and frailty.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112600"},"PeriodicalIF":3.9,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcopenic obesity and osteoporosis: Research progress and hot spots.","authors":"Shangheng Fan, Yulan Cai, Yunqin Wei, Jia Yang, Jianmei Gao, Yan Yang","doi":"10.1016/j.exger.2024.112544","DOIUrl":"10.1016/j.exger.2024.112544","url":null,"abstract":"<p><p>Sarcopenic obesity (SO) and osteoporosis (OP) are associated with aging and obesity. The pathogenesis of SO is complex, including glucolipid and skeletal muscle metabolic disorders caused by inflammation, insulin resistance, and other factors. Growing evidence links muscle damage to bone loss. Muscle-lipid metabolism disorders of SO disrupt the balance between bone formation and bone resorption, increasing the risk of OP. Conversely, bones also play a role in fat and muscle metabolism. In the context of aging and obesity, the comprehensive review focuses on the effects of mechanical stimulation, mesenchymal stem cells (MSCs), chronic inflammation, myokines, and adipokines on musculoskeletal, at the same time, the impact of osteokines on muscle-lipid metabolism were also analyzed. So far, exercise combined with diet therapy is the most effective strategy for increasing musculoskeletal mass. A holistic treatment of musculoskeletal diseases is still in the preliminary exploration stage. Therefore, this article aims to improve the understanding of musculoskeletal -fat interactions in SO and OP, explores targets that can provide holistic treatment for SO combined with OP, and discusses current limitations and challenges. We hope to provide relevant ideas for developing specific therapies and improving disease prognosis in the future.</p>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":" ","pages":"112544"},"PeriodicalIF":3.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating small non-coding RNA profiling for identification of older adults with low muscle strength and physical performance: A preliminary study","authors":"Hyung Eun Shin ,&nbsp;Chang Won Won ,&nbsp;Miji Kim","doi":"10.1016/j.exger.2024.112598","DOIUrl":"10.1016/j.exger.2024.112598","url":null,"abstract":"<div><h3>Background</h3><div>Small non-coding RNAs (ncRNAs) have recently emerged as potential biomarkers of sarcopenia. However, previous studies have rarely explored the association of small ncRNAs with sarcopenic components, especially muscle strength and physical performance. We aimed to examine circulating small ncRNA profiles to detect low muscle strength and physical performance in older adults.</div></div><div><h3>Methods</h3><div>Ninety-eight older adults were randomly selected from Korean Frailty and Aging Cohort Study and classified into the “Normal,” “Low muscle strength (MS) only,” “Low physical performance (PP) only,” and “Low MS and PP” groups by Asian Working Group for Sarcopenia 2019 criteria. We used high-throughput sequencing to delineate small ncRNA profiles in plasma. Differentially expressed small ncRNAs were analyzed to reveal distinct patterns based on muscle strength and physical performance status.</div></div><div><h3>Results</h3><div>In “Low MS and PP” group, 119 miRNAs, 86 piRNAs, 92 snoRNAs, 106 snRNAs, and 15 tRNAs were differentially expressed compared to “Normal” group (<em>p</em> &lt; 0.05). After Benjamini-Hochberg adjustment, 39 miRNAs, 2 piRNAs, 75 snoRNAs, 48 snRNAs, and 15 tRNAs showed differential expression in “Low MS and PP” group compared to than “Normal” group (adjusted <em>p</em> &lt; 0.05). No significant differences were observed in comparisons between the other groups (adjusted <em>p</em> &gt; 0.05).</div></div><div><h3>Conclusion</h3><div>The expression of circulating small ncRNAs were comprehensively characterized, revealing distinct signatures in older adults with both low muscle strength and physical performance compared to normal individuals. Although preliminary, this characterization can advance small ncRNA research on age-related declines in muscle strength and physical performance by providing foundational data for further investigation.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112598"},"PeriodicalIF":3.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mitochondrial function of peripheral blood mononuclear cells in frail older patients","authors":"Tingting Huang ,&nbsp;Li Qin ,&nbsp;Danmei Zhang,&nbsp;Qiangwei Tong,&nbsp;Qianqian Zhu,&nbsp;Guoxian Ding,&nbsp;Juan Liu","doi":"10.1016/j.exger.2024.112594","DOIUrl":"10.1016/j.exger.2024.112594","url":null,"abstract":"<div><h3>Background</h3><div>Frailty increases the incidence of geriatric syndromes and even the risk of death in old adults. However, the diagnostic criteria for frailty are inconsistent because of complex pathological processes and diverse clinical manifestations. To determine the effective biomarker and recognize frail status early, we investigated the correlation of mitochondrial morphology and function of human peripheral blood mononuclear cells (PBMCs) with frailty status in older adults.</div></div><div><h3>Methods</h3><div>This Cross-sectional study followed 393 participants (aged 25–100 years, female 31.04 %) from the First Affiliated Hospital of Nanjing Medical University. The frailty status of subjects was assessed by the physical frailty phenotype (PFP) scale. We analyzed mitochondria functions including mitochondria copy number (mtDNAcn), the mRNA expressions of mitochondrial dynamics-related genes mitofusin 1(MFN1), mitofusin 2(MFN2), optic atrophy protein-1(OPA1), fission protein-1(FIS1) and dynamin-related protein 1(DRP1), mitochondrial oxidative respiration and reactive oxygen species(ROS) levels in PBMCs. Mitochondria morphology, size, and number were observed by transmission electron microscopy (TEM).</div></div><div><h3>Results</h3><div>After adjustment for sex and BMI, mtDNAcn, the mRNA expression of FIS1, mitochondrial respiratory function (proton leak, maximum oxygen consumption, and respiratory reserve) and ROS level were significantly correlated with age (<em>P</em> = 0.031, 0.030, 0.042, 0.003, 0.002, 0.022, respectively). After correcting for age, sex, and BMI, mtDNAcn and the mRNA expression of OPA1 were correlated with 4 m gait speed respectively (<em>P</em> = 0.003, 0.028, respectively). Compared with non-frail people, mtDNAcn, the mRNA expression of MFN1, mitochondrial basal respiration, proton leak, maximum oxygen consumption, ATP production and space capacity were significantly decreased in frail older adults (<em>P</em> = 0.013, 0.036, 0.026, 0.024, 0.012, 0.032, 0.020, respectively). ROS levels were significantly increased in the frail group (<em>P</em> = 0.016). Compared with non-frail people, the number, length, and perimeter, area of mitochondria were reduced in frail group under TEM (all <em>P</em> &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Mitochondrial dysfunctions (decreased mtDNAcn, impaired mitochondrial morphology, imbalanced mitochondrial dynamic, impaired mitochondrial respiratory function, and increased ROS levels) were significantly correlated with frail status.</div></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":"197 ","pages":"Article 112594"},"PeriodicalIF":3.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}